Standard name | |
---|---|
Human Ortholog | |
Description | Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 1 | 2 | 5 | 15 | 26 | 28 | 16 | – | 29 | 53 | 0 | 0 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
Bud | 1 | 2 | 1 | 0 | 1 | 1 | 0 | 2 | 2 | – | 9 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 30 | 16 | 14 | 14 | 32 | 34 | 51 | 37 | 34 | – | 59 | 77 | 8 | 4 | 18 | 4 | 17 | 5 | 0 | 0 | 1 | 1 | 1 | 0 |
Cytoplasm | 512 | 382 | 378 | 320 | 401 | 433 | 501 | 433 | 275 | – | 287 | 271 | 273 | 293 | 319 | 311 | 170 | 232 | 529 | 365 | 373 | 193 | 248 | 285 |
Endoplasmic Reticulum | 17 | 11 | 14 | 8 | 72 | 52 | 120 | 107 | 70 | – | 47 | 102 | 9 | 7 | 6 | 19 | 3 | 9 | 7 | 6 | 16 | 12 | 14 | 26 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | – | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 1 | 1 | 1 | 0 | 0 | 1 | 6 | 5 | 2 | – | 2 | 1 | 0 | 0 | 0 | 3 | 1 | 2 | 1 | 0 | 1 | 0 | 2 | 0 |
Mitochondria | 1 | 2 | 1 | 9 | 10 | 55 | 73 | 70 | 88 | – | 129 | 153 | 1 | 1 | 9 | 5 | 1 | 4 | 2 | 1 | 3 | 1 | 0 | 0 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | – | 2 | 1 | 0 | 2 | 3 | 6 | 3 | 5 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 2 | 2 | – | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 7 | 1 | 4 | 5 | 0 | 1 | 12 | 37 | 5 | – | 10 | 10 | 3 | 2 | 5 | 17 | 14 | 19 | 4 | 4 | 4 | 3 | 2 | 2 |
Unique Cell Count | 542 | 391 | 394 | 339 | 481 | 525 | 697 | 651 | 419 | 466 | 513 | 284 | 303 | 337 | 337 | 196 | 255 | 552 | 382 | 407 | 215 | 279 | 327 | |
Labelled Cell Count | 572 | 415 | 414 | 358 | 521 | 592 | 794 | 726 | 494 | 576 | 672 | 296 | 311 | 367 | 366 | 210 | 277 | 552 | 382 | 407 | 215 | 279 | 327 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 20.3 | 17.9 | 14.5 | 14.0 | 15.4 | 14.9 | 14.7 | 14.3 | 14.1 | – | 14.1 | 13.3 | 16.6 | 17.7 | 16.5 | 21.2 | 24.4 | 24.0 | 17.1 | 17.8 | 20.2 |
Std Deviation (1e-4) | 4.0 | 3.2 | 2.3 | 2.4 | 2.5 | 2.8 | 2.8 | 3.1 | 3.2 | – | 3.6 | 3.3 | 4.0 | 4.4 | 4.2 | 6.7 | 8.3 | 9.3 | 3.2 | 4.4 | 5.6 |
Intensity Change (Log2) | – | – | – | -0.05 | 0.08 | 0.04 | 0.01 | -0.02 | -0.05 | – | -0.05 | -0.13 | 0.19 | 0.29 | 0.18 | 0.55 | 0.75 | 0.72 | 0.23 | 0.29 | 0.48 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 3.0 | 3.6 | 3.9 | 3.6 | – | 4.8 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.4 | 2.0 | 2.0 | 2.5 | 1.5 | 2.8 | – | 4.8 | 5.7 | -0.5 | -1.8 | 1.2 | -2.1 | 2.6 | -1.2 |
Cytoplasm | -1.0 | -5.9 | -6.3 | -9.6 | -11.1 | -10.9 | – | -12.0 | -14.3 | 0.1 | 0.5 | -0.8 | -2.1 | -4.1 | -2.6 |
Endoplasmic Reticulum | -0.9 | 5.6 | 3.7 | 6.6 | 6.3 | 6.2 | – | 3.7 | 7.3 | -0.3 | -1.0 | -1.5 | 1.4 | -1.4 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 6.4 | 6.4 | 6.5 | 9.5 | – | 11.2 | 11.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0.9 | 3.8 | 0 | – | 1.3 | 1.1 | 0 | 0 | 0 | 3.3 | 4.1 | 4.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 18.7701 | 26.0802 | 25.1737 | 20.9598 | 21.263 | 25.6367 | 12.3156 | 20.1241 | 18.5451 | 16.9484 | 15.6098 | 18.0772 | 13.881 | 17.4431 | 16.5271 | 16.4893 | 15.2309 | 16.3892 |
Actin | 0.0018 | 0.0009 | 0.0014 | 0.0097 | 0.0007 | 0.0011 | 0.0646 | 0.0005 | 0.0088 | 0.0006 | 0.0008 | 0.0006 | 0.0015 | 0.0011 | 0.0149 | 0.0002 | 0.0051 | 0.0096 |
Bud | 0.0006 | 0.001 | 0.0014 | 0.0051 | 0.0005 | 0.001 | 0.0009 | 0.0002 | 0.0004 | 0.0008 | 0.0004 | 0.0001 | 0.0005 | 0.0003 | 0.0008 | 0.0008 | 0.0002 | 0.0003 |
Bud Neck | 0.0009 | 0.001 | 0.0009 | 0.0027 | 0.0014 | 0.0043 | 0.0061 | 0.0003 | 0.0004 | 0.0004 | 0.0009 | 0.0009 | 0.0002 | 0.0002 | 0.0007 | 0.0008 | 0.0005 | 0.0015 |
Bud Periphery | 0.0009 | 0.0008 | 0.0017 | 0.0156 | 0.0003 | 0.0023 | 0.0008 | 0 | 0.0002 | 0.0003 | 0.0002 | 0 | 0.0009 | 0 | 0.0005 | 0.0002 | 0.0001 | 0.0002 |
Bud Site | 0.0007 | 0.008 | 0.0006 | 0.0014 | 0.0001 | 0.0002 | 0.0046 | 0.0045 | 0.0008 | 0.0005 | 0.0004 | 0 | 0.0005 | 0.0009 | 0.0074 | 0.0004 | 0.0006 | 0.0001 |
Cell Periphery | 0.004 | 0.0074 | 0.0018 | 0.011 | 0.0007 | 0.0013 | 0.0006 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0003 | 0.0002 | 0.0004 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.7887 | 0.8996 | 0.8589 | 0.6365 | 0.8418 | 0.8396 | 0.6449 | 0.9719 | 0.9159 | 0.8519 | 0.8724 | 0.9316 | 0.8409 | 0.9607 | 0.9141 | 0.9031 | 0.8485 | 0.868 |
Cytoplasmic Foci | 0.0024 | 0.002 | 0.0026 | 0.005 | 0.0061 | 0.0041 | 0.0345 | 0.0026 | 0.0069 | 0.0087 | 0.0184 | 0.0057 | 0.006 | 0.0055 | 0.0115 | 0.009 | 0.0432 | 0.0153 |
Eisosomes | 0.0003 | 0 | 0 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.1272 | 0.029 | 0.0529 | 0.076 | 0.044 | 0.0898 | 0.0387 | 0.0123 | 0.0133 | 0.0275 | 0.0085 | 0.0326 | 0.0238 | 0.0189 | 0.0061 | 0.0042 | 0.0087 | 0.0396 |
Endosome | 0.011 | 0.0045 | 0.0094 | 0.0257 | 0.0234 | 0.0114 | 0.0524 | 0.0028 | 0.0181 | 0.0592 | 0.0569 | 0.0181 | 0.0148 | 0.0062 | 0.0261 | 0.0227 | 0.0487 | 0.0275 |
Golgi | 0.0013 | 0.0012 | 0.0012 | 0.0032 | 0.0021 | 0.0025 | 0.0136 | 0.0004 | 0.009 | 0.002 | 0.0145 | 0.0015 | 0.0034 | 0.0009 | 0.0024 | 0.0008 | 0.0081 | 0.0107 |
Lipid Particles | 0.0008 | 0.0012 | 0.0004 | 0.0011 | 0.0001 | 0.0001 | 0.0219 | 0.0001 | 0.0017 | 0.0002 | 0.0017 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0.0001 | 0.0166 | 0.0029 |
Mitochondria | 0.004 | 0.0006 | 0.0033 | 0.0329 | 0.0265 | 0.0184 | 0.0043 | 0.0001 | 0.0047 | 0.0011 | 0.0118 | 0.0006 | 0.0932 | 0.0001 | 0.0012 | 0.0002 | 0.0012 | 0.0095 |
None | 0.0118 | 0.0003 | 0.0004 | 0.0006 | 0.0003 | 0.0003 | 0.0066 | 0.0003 | 0.0006 | 0.0006 | 0.0003 | 0.0004 | 0.0031 | 0.0006 | 0.0013 | 0.0003 | 0.0027 | 0.0004 |
Nuclear Periphery | 0.0098 | 0.0024 | 0.0052 | 0.0112 | 0.0049 | 0.0029 | 0.0435 | 0.0011 | 0.0017 | 0.0041 | 0.0009 | 0.0026 | 0.0023 | 0.0011 | 0.0017 | 0.0018 | 0.0035 | 0.001 |
Nucleolus | 0.0004 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0.0007 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 |
Nucleus | 0.0041 | 0.0021 | 0.0024 | 0.0038 | 0.0023 | 0.0019 | 0.0087 | 0.0006 | 0.0011 | 0.0022 | 0.001 | 0.0013 | 0.0015 | 0.0007 | 0.0022 | 0.0013 | 0.0007 | 0.0007 |
Peroxisomes | 0.0001 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0096 | 0 | 0.0011 | 0.0001 | 0.0012 | 0 | 0.0004 | 0 | 0.0004 | 0.0001 | 0.0022 | 0.0096 |
Punctate Nuclear | 0.0008 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0251 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0055 | 0.0001 |
Vacuole | 0.025 | 0.0349 | 0.0499 | 0.1406 | 0.0348 | 0.0166 | 0.0133 | 0.0018 | 0.0132 | 0.0348 | 0.0066 | 0.0026 | 0.0046 | 0.0022 | 0.005 | 0.0427 | 0.0026 | 0.0021 |
Vacuole Periphery | 0.0035 | 0.0024 | 0.0057 | 0.0174 | 0.0097 | 0.0022 | 0.004 | 0.0003 | 0.0016 | 0.0046 | 0.0027 | 0.0009 | 0.0017 | 0.0003 | 0.001 | 0.0109 | 0.0011 | 0.0009 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 60.4182 | 53.8788 | 53.8273 | 74.7644 | 49.3643 | 64.9394 | 73.0122 | 75.0609 | 72.2768 | 77.2944 |
Translational Efficiency | 0.8301 | 0.9108 | 0.7314 | 0.5089 | 0.7347 | 0.8726 | 0.6734 | 0.6302 | 0.6166 | 0.5741 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1354 | 222 | 230 | 1181 | 2173 | 2236 | 210 | 1041 | 3527 | 2458 | 440 | 2222 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1211.01 | 1632.35 | 2834.90 | 1888.02 | 1365.58 | 1461.19 | 2040.88 | 1871.56 | 1306.24 | 1476.65 | 2455.94 | 1880.31 |
Standard Deviation | 222.79 | 232.96 | 355.43 | 334.08 | 214.42 | 240.69 | 243.94 | 338.56 | 230.29 | 244.97 | 501.73 | 336.29 |
Intensity Change Log 2 | — | 0.430740 | 1.227087 | 0.640663 | — | 0.097630 | 0.579678 | 0.454727 | — | 0.263796 | 0.920170 | 0.545109 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000169 | 0.000612 | 0.000876 | 0.000385 | 0.000142 | 0.000204 | 0.001922 | 0.000599 | 0.000152 | 0.000241 | 0.001375 | 0.000485 |
Bud Neck | 0.000196 | 0.000827 | 0.014330 | 0.002292 | 0.000142 | 0.000198 | 0.007185 | 0.001735 | 0.000163 | 0.000255 | 0.010920 | 0.002031 |
Bud Site | 0.013197 | 0.030569 | 0.019599 | 0.027492 | 0.010310 | 0.009654 | 0.042984 | 0.022069 | 0.011418 | 0.011543 | 0.030760 | 0.024951 |
Cell Periphery | 0.000281 | 0.001006 | 0.009830 | 0.001517 | 0.000376 | 0.000228 | 0.005285 | 0.004386 | 0.000340 | 0.000298 | 0.007661 | 0.002861 |
Cytoplasm | 0.892171* | 0.828604* | 0.360735* | 0.725219* | 0.910605* | 0.906700* | 0.628245* | 0.792683* | 0.903529* | 0.899647* | 0.488410* | 0.756826* |
Cytoplasmic Foci | 0.050982 | 0.079339 | 0.029775 | 0.023611 | 0.048254 | 0.047358 | 0.058019 | 0.020799 | 0.049301 | 0.050246 | 0.043255 | 0.022294 |
Eisosomes | 0.000002 | 0.000004 | 0.000012 | 0.000004 | 0.000002 | 0.000003 | 0.000014 | 0.000004 | 0.000002 | 0.000003 | 0.000013 | 0.000004 |
Endoplasmic Reticulum | 0.002899 | 0.007563 | 0.001802 | 0.000979 | 0.002973 | 0.001269 | 0.005972 | 0.001045 | 0.002945 | 0.001837 | 0.003792 | 0.001010 |
Endosome | 0.002345 | 0.005401 | 0.010810 | 0.006528 | 0.001441 | 0.002984 | 0.015289 | 0.005459 | 0.001788 | 0.003203 | 0.012948 | 0.006027 |
Golgi | 0.009347 | 0.018881 | 0.033460 | 0.013332 | 0.010102 | 0.012762 | 0.020540 | 0.008815 | 0.009812 | 0.013314 | 0.027294 | 0.011216 |
Lipid Particles | 0.000476 | 0.002887 | 0.031343 | 0.009753 | 0.000958 | 0.001153 | 0.033034 | 0.012457 | 0.000773 | 0.001310 | 0.032150 | 0.011020 |
Mitochondria | 0.000181 | 0.000158 | 0.000072 | 0.000437 | 0.000234 | 0.000579 | 0.000026 | 0.000372 | 0.000214 | 0.000541 | 0.000050 | 0.000407 |
Mitotic Spindle | 0.000414 | 0.002924 | 0.007417 | 0.005618 | 0.000469 | 0.001809 | 0.006684 | 0.002041 | 0.000448 | 0.001909 | 0.007067 | 0.003942 |
None | 0.001316 | 0.000278 | 0.000400 | 0.000347 | 0.000609 | 0.000928 | 0.000223 | 0.000697 | 0.000880 | 0.000870 | 0.000315 | 0.000511 |
Nuclear Periphery | 0.000037 | 0.000039 | 0.001157 | 0.000113 | 0.000008 | 0.000011 | 0.000187 | 0.000102 | 0.000019 | 0.000014 | 0.000694 | 0.000108 |
Nuclear Periphery Foci | 0.001081 | 0.001775 | 0.022330 | 0.003034 | 0.000463 | 0.000609 | 0.013022 | 0.002815 | 0.000701 | 0.000714 | 0.017887 | 0.002931 |
Nucleolus | 0.000172 | 0.000014 | 0.000101 | 0.000157 | 0.000018 | 0.000092 | 0.000055 | 0.000122 | 0.000077 | 0.000085 | 0.000079 | 0.000141 |
Nucleus | 0.003060 | 0.001690 | 0.015962 | 0.005678 | 0.001430 | 0.002615 | 0.007526 | 0.004374 | 0.002056 | 0.002532 | 0.011936 | 0.005067 |
Peroxisomes | 0.000153 | 0.000202 | 0.000140 | 0.000483 | 0.000109 | 0.000303 | 0.000364 | 0.000420 | 0.000126 | 0.000294 | 0.000247 | 0.000453 |
Vacuole | 0.009300 | 0.010389 | 0.416517* | 0.160355 | 0.004591 | 0.006085 | 0.146690 | 0.109577 | 0.006398 | 0.006474 | 0.287736* | 0.136566 |
Vacuole Periphery | 0.012221 | 0.006837 | 0.023333 | 0.012666 | 0.006762 | 0.004455 | 0.006733 | 0.009426 | 0.008858 | 0.004670 | 0.015410 | 0.011148 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -3.87 | -7.39 | -6.58 | 1.74 | 5.17 | -1.72 | -7.79 | -3.43 | -2.94 | 4.92 | -2.66 | -10.23 | -5.21 | -3.64 | 6.60 |
Bud Neck | -3.52 | -12.05 | -14.48 | -7.73 | 10.13 | -3.24 | -10.37 | -13.40 | -12.95 | 7.62 | -3.91 | -14.81 | -19.61 | -18.57 | 11.99 |
Bud Site | -3.64 | -3.18 | -6.93 | 0.63 | -2.15 | 0.74 | -7.50 | -6.80 | -7.19 | 4.33 | -0.16 | -8.07 | -10.07 | -9.44 | 2.30 |
Cell Periphery | -2.72 | -7.85 | -9.55 | -1.43 | 6.79 | 3.88 | -5.54 | -6.49 | -6.77 | 1.07 | 1.13 | -9.51 | -8.46 | -8.60 | 5.91 |
Cytoplasm | 3.94 | 24.66 | 18.85 | 6.59 | -15.66 | 0.81 | 15.04 | 15.63 | 14.89 | -8.00 | 0.88 | 26.56 | 25.09 | 22.85 | -15.88 |
Cytoplasmic Foci | -3.51 | 3.87 | 9.20 | 7.20 | 2.69 | 0.31 | -2.43 | 11.45 | 11.42 | 8.14 | -0.38 | 0.79 | 14.40 | 13.04 | 7.74 |
Eisosomes | -2.43 | -8.83 | -5.24 | 0.04 | 7.15 | -0.88 | -9.53 | -5.00 | -4.10 | 7.44 | -1.74 | -13.02 | -7.58 | -5.02 | 10.33 |
Endoplasmic Reticulum | -1.45 | 2.10 | 4.14 | 2.10 | 2.21 | 2.67 | -1.53 | 3.06 | 0.82 | 2.81 | 2.05 | -0.71 | 4.64 | 2.32 | 3.24 |
Endosome | -2.85 | -9.33 | -6.23 | -0.69 | 4.89 | -3.78 | -9.63 | -5.21 | -2.77 | 6.23 | -3.60 | -13.48 | -8.54 | -4.42 | 7.89 |
Golgi | -4.27 | -6.74 | -1.72 | 2.79 | 5.57 | -2.46 | -4.71 | 2.12 | 3.77 | 5.45 | -3.63 | -8.07 | -0.30 | 2.72 | 7.47 |
Lipid Particles | -4.14 | -11.30 | -9.33 | -6.25 | 7.45 | -0.86 | -8.84 | -9.01 | -8.76 | 5.42 | -2.58 | -14.10 | -12.87 | -11.91 | 9.03 |
Mitochondria | 0.25 | 3.93 | -1.23 | -1.22 | -1.92 | -1.80 | 7.13 | -0.67 | 1.06 | -2.09 | -1.88 | 7.74 | -1.30 | 0.95 | -2.82 |
Mitotic Spindle | -2.76 | -6.25 | -3.95 | -1.66 | 1.70 | -2.76 | -4.91 | -2.78 | -0.56 | 3.26 | -3.27 | -7.91 | -4.72 | -2.40 | 3.28 |
None | 2.84 | 2.49 | 2.62 | -0.89 | 0.61 | -1.42 | 5.32 | -0.08 | 0.57 | -0.93 | 0.04 | 3.81 | 1.53 | 1.32 | -0.79 |
Nuclear Periphery | -0.09 | -8.61 | -2.94 | -2.63 | 7.90 | -1.08 | -9.83 | -2.35 | -2.28 | 2.11 | 1.14 | -9.33 | -3.90 | -4.19 | 7.79 |
Nuclear Periphery Foci | -2.04 | -9.62 | -4.74 | -2.99 | 8.45 | -0.78 | -11.40 | -11.23 | -8.05 | 9.01 | -0.12 | -13.40 | -9.18 | -7.79 | 11.29 |
Nucleolus | 1.65 | 0.62 | 0.15 | -13.43 | -3.28 | -1.27 | -6.48 | -10.53 | -0.67 | -5.81 | -0.10 | -0.26 | -1.78 | -1.16 | -6.18 |
Nucleus | 3.22 | -6.70 | -6.33 | -9.44 | 4.89 | -2.81 | -10.71 | -10.77 | -5.79 | 3.47 | -1.19 | -9.02 | -11.74 | -8.14 | 5.50 |
Peroxisomes | -0.82 | -0.15 | -5.06 | -5.93 | -7.16 | -2.21 | -5.29 | -8.10 | -1.08 | -0.69 | -2.04 | -4.10 | -9.72 | -1.67 | -4.84 |
Vacuole | -0.33 | -18.73 | -25.01 | -22.55 | 10.63 | -2.16 | -10.57 | -21.08 | -20.81 | 2.27 | -0.15 | -19.51 | -32.60 | -32.57 | 9.32 |
Vacuole Periphery | 1.99 | -2.39 | 0.56 | -1.81 | 2.99 | 2.93 | 0.30 | -1.46 | -3.66 | -1.30 | 4.97 | -2.52 | -1.09 | -6.41 | 1.87 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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