Standard name
Human Ortholog
Description Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.06 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.06 0 0 0 0.07 0.06 0.07 0.06 0.08 0.13 0.15 0 0 0.05 0 0.09 0 0 0 0 0 0 0
Cytoplasm 0.94 0.98 0.96 0.94 0.83 0.82 0.72 0.67 0.66 0.62 0.53 0.96 0.97 0.95 0.92 0.87 0.91 0.96 0.96 0.92 0.9 0.89 0.87
Endoplasmic Reticulum 0 0 0 0 0.15 0.1 0.17 0.16 0.17 0.1 0.2 0 0 0 0.06 0 0 0 0 0 0.06 0.05 0.08
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.1 0.11 0.21 0.28 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0.05 0.07 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 1 2 5 15 26 28 16 29 53 0 0 5 0 0 0 0 0 0 0 2 1
Bud 1 2 1 0 1 1 0 2 2 9 1 2 0 1 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 30 16 14 14 32 34 51 37 34 59 77 8 4 18 4 17 5 0 0 1 1 1 0
Cytoplasm 512 382 378 320 401 433 501 433 275 287 271 273 293 319 311 170 232 529 365 373 193 248 285
Endoplasmic Reticulum 17 11 14 8 72 52 120 107 70 47 102 9 7 6 19 3 9 7 6 16 12 14 26
Endosome 0 0 0 0 0 0 0 2 0 0 0 0 1 1 1 1 0 0 0 0 0 0 0
Golgi 1 1 1 0 0 1 6 5 2 2 1 0 0 0 3 1 2 1 0 1 0 2 0
Mitochondria 1 2 1 9 10 55 73 70 88 129 153 1 1 9 5 1 4 2 1 3 1 0 0
Nucleus 1 0 0 0 0 0 2 3 0 2 1 0 2 3 6 3 5 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 3 2 2 2 2 0 0 0 0 0 0 2 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 1 0 0 1 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 7 1 4 5 0 1 12 37 5 10 10 3 2 5 17 14 19 4 4 4 3 2 2
Unique Cell Count 542 391 394 339 481 525 697 651 419 466 513 284 303 337 337 196 255 552 382 407 215 279 327
Labelled Cell Count 572 415 414 358 521 592 794 726 494 576 672 296 311 367 366 210 277 552 382 407 215 279 327


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 20.3 17.9 14.5 14.0 15.4 14.9 14.7 14.3 14.1 14.1 13.3 16.6 17.7 16.5 21.2 24.4 24.0 17.1 17.8 20.2
Std Deviation (1e-4) 4.0 3.2 2.3 2.4 2.5 2.8 2.8 3.1 3.2 3.6 3.3 4.0 4.4 4.2 6.7 8.3 9.3 3.2 4.4 5.6
Intensity Change (Log2) -0.05 0.08 0.04 0.01 -0.02 -0.05 -0.05 -0.13 0.19 0.29 0.18 0.55 0.75 0.72 0.23 0.29 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 3.0 3.6 3.9 3.6 4.8 6.4 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.4 2.0 2.0 2.5 1.5 2.8 4.8 5.7 -0.5 -1.8 1.2 -2.1 2.6 -1.2
Cytoplasm -1.0 -5.9 -6.3 -9.6 -11.1 -10.9 -12.0 -14.3 0.1 0.5 -0.8 -2.1 -4.1 -2.6
Endoplasmic Reticulum -0.9 5.6 3.7 6.6 6.3 6.2 3.7 7.3 -0.3 -1.0 -1.5 1.4 -1.4 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.4 6.4 6.5 9.5 11.2 11.8 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0.9 3.8 0 1.3 1.1 0 0 0 3.3 4.1 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.7701 26.0802 25.1737 20.9598 21.263 25.6367 12.3156 20.1241 18.5451 16.9484 15.6098 18.0772 13.881 17.4431 16.5271 16.4893 15.2309 16.3892
Actin 0.0018 0.0009 0.0014 0.0097 0.0007 0.0011 0.0646 0.0005 0.0088 0.0006 0.0008 0.0006 0.0015 0.0011 0.0149 0.0002 0.0051 0.0096
Bud 0.0006 0.001 0.0014 0.0051 0.0005 0.001 0.0009 0.0002 0.0004 0.0008 0.0004 0.0001 0.0005 0.0003 0.0008 0.0008 0.0002 0.0003
Bud Neck 0.0009 0.001 0.0009 0.0027 0.0014 0.0043 0.0061 0.0003 0.0004 0.0004 0.0009 0.0009 0.0002 0.0002 0.0007 0.0008 0.0005 0.0015
Bud Periphery 0.0009 0.0008 0.0017 0.0156 0.0003 0.0023 0.0008 0 0.0002 0.0003 0.0002 0 0.0009 0 0.0005 0.0002 0.0001 0.0002
Bud Site 0.0007 0.008 0.0006 0.0014 0.0001 0.0002 0.0046 0.0045 0.0008 0.0005 0.0004 0 0.0005 0.0009 0.0074 0.0004 0.0006 0.0001
Cell Periphery 0.004 0.0074 0.0018 0.011 0.0007 0.0013 0.0006 0.0003 0.0002 0.0001 0.0001 0 0.0003 0.0002 0.0004 0.0003 0.0001 0.0001
Cytoplasm 0.7887 0.8996 0.8589 0.6365 0.8418 0.8396 0.6449 0.9719 0.9159 0.8519 0.8724 0.9316 0.8409 0.9607 0.9141 0.9031 0.8485 0.868
Cytoplasmic Foci 0.0024 0.002 0.0026 0.005 0.0061 0.0041 0.0345 0.0026 0.0069 0.0087 0.0184 0.0057 0.006 0.0055 0.0115 0.009 0.0432 0.0153
Eisosomes 0.0003 0 0 0.0001 0 0 0.0005 0 0.0001 0 0 0 0 0 0.0003 0 0.0001 0
Endoplasmic Reticulum 0.1272 0.029 0.0529 0.076 0.044 0.0898 0.0387 0.0123 0.0133 0.0275 0.0085 0.0326 0.0238 0.0189 0.0061 0.0042 0.0087 0.0396
Endosome 0.011 0.0045 0.0094 0.0257 0.0234 0.0114 0.0524 0.0028 0.0181 0.0592 0.0569 0.0181 0.0148 0.0062 0.0261 0.0227 0.0487 0.0275
Golgi 0.0013 0.0012 0.0012 0.0032 0.0021 0.0025 0.0136 0.0004 0.009 0.002 0.0145 0.0015 0.0034 0.0009 0.0024 0.0008 0.0081 0.0107
Lipid Particles 0.0008 0.0012 0.0004 0.0011 0.0001 0.0001 0.0219 0.0001 0.0017 0.0002 0.0017 0.0001 0.0002 0.0001 0.001 0.0001 0.0166 0.0029
Mitochondria 0.004 0.0006 0.0033 0.0329 0.0265 0.0184 0.0043 0.0001 0.0047 0.0011 0.0118 0.0006 0.0932 0.0001 0.0012 0.0002 0.0012 0.0095
None 0.0118 0.0003 0.0004 0.0006 0.0003 0.0003 0.0066 0.0003 0.0006 0.0006 0.0003 0.0004 0.0031 0.0006 0.0013 0.0003 0.0027 0.0004
Nuclear Periphery 0.0098 0.0024 0.0052 0.0112 0.0049 0.0029 0.0435 0.0011 0.0017 0.0041 0.0009 0.0026 0.0023 0.0011 0.0017 0.0018 0.0035 0.001
Nucleolus 0.0004 0.0003 0 0.0002 0 0 0.0007 0 0 0 0.0001 0 0 0 0.0004 0 0.0001 0
Nucleus 0.0041 0.0021 0.0024 0.0038 0.0023 0.0019 0.0087 0.0006 0.0011 0.0022 0.001 0.0013 0.0015 0.0007 0.0022 0.0013 0.0007 0.0007
Peroxisomes 0.0001 0 0 0.0002 0.0001 0.0001 0.0096 0 0.0011 0.0001 0.0012 0 0.0004 0 0.0004 0.0001 0.0022 0.0096
Punctate Nuclear 0.0008 0.0001 0.0001 0.0002 0.0001 0 0.0251 0 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0009 0.0001 0.0055 0.0001
Vacuole 0.025 0.0349 0.0499 0.1406 0.0348 0.0166 0.0133 0.0018 0.0132 0.0348 0.0066 0.0026 0.0046 0.0022 0.005 0.0427 0.0026 0.0021
Vacuole Periphery 0.0035 0.0024 0.0057 0.0174 0.0097 0.0022 0.004 0.0003 0.0016 0.0046 0.0027 0.0009 0.0017 0.0003 0.001 0.0109 0.0011 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 60.4182 53.8788 53.8273 74.7644 49.3643 64.9394 73.0122 75.0609 72.2768 77.2944
Translational Efficiency 0.8301 0.9108 0.7314 0.5089 0.7347 0.8726 0.6734 0.6302 0.6166 0.5741

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1354 222 230 1181 2173 2236 210 1041 3527 2458 440 2222

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1211.01 1632.35 2834.90 1888.02 1365.58 1461.19 2040.88 1871.56 1306.24 1476.65 2455.94 1880.31
Standard Deviation 222.79 232.96 355.43 334.08 214.42 240.69 243.94 338.56 230.29 244.97 501.73 336.29
Intensity Change Log 2 0.430740 1.227087 0.640663 0.097630 0.579678 0.454727 0.263796 0.920170 0.545109

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000169 0.000612 0.000876 0.000385 0.000142 0.000204 0.001922 0.000599 0.000152 0.000241 0.001375 0.000485
Bud Neck 0.000196 0.000827 0.014330 0.002292 0.000142 0.000198 0.007185 0.001735 0.000163 0.000255 0.010920 0.002031
Bud Site 0.013197 0.030569 0.019599 0.027492 0.010310 0.009654 0.042984 0.022069 0.011418 0.011543 0.030760 0.024951
Cell Periphery 0.000281 0.001006 0.009830 0.001517 0.000376 0.000228 0.005285 0.004386 0.000340 0.000298 0.007661 0.002861
Cytoplasm 0.892171* 0.828604* 0.360735* 0.725219* 0.910605* 0.906700* 0.628245* 0.792683* 0.903529* 0.899647* 0.488410* 0.756826*
Cytoplasmic Foci 0.050982 0.079339 0.029775 0.023611 0.048254 0.047358 0.058019 0.020799 0.049301 0.050246 0.043255 0.022294
Eisosomes 0.000002 0.000004 0.000012 0.000004 0.000002 0.000003 0.000014 0.000004 0.000002 0.000003 0.000013 0.000004
Endoplasmic Reticulum 0.002899 0.007563 0.001802 0.000979 0.002973 0.001269 0.005972 0.001045 0.002945 0.001837 0.003792 0.001010
Endosome 0.002345 0.005401 0.010810 0.006528 0.001441 0.002984 0.015289 0.005459 0.001788 0.003203 0.012948 0.006027
Golgi 0.009347 0.018881 0.033460 0.013332 0.010102 0.012762 0.020540 0.008815 0.009812 0.013314 0.027294 0.011216
Lipid Particles 0.000476 0.002887 0.031343 0.009753 0.000958 0.001153 0.033034 0.012457 0.000773 0.001310 0.032150 0.011020
Mitochondria 0.000181 0.000158 0.000072 0.000437 0.000234 0.000579 0.000026 0.000372 0.000214 0.000541 0.000050 0.000407
Mitotic Spindle 0.000414 0.002924 0.007417 0.005618 0.000469 0.001809 0.006684 0.002041 0.000448 0.001909 0.007067 0.003942
None 0.001316 0.000278 0.000400 0.000347 0.000609 0.000928 0.000223 0.000697 0.000880 0.000870 0.000315 0.000511
Nuclear Periphery 0.000037 0.000039 0.001157 0.000113 0.000008 0.000011 0.000187 0.000102 0.000019 0.000014 0.000694 0.000108
Nuclear Periphery Foci 0.001081 0.001775 0.022330 0.003034 0.000463 0.000609 0.013022 0.002815 0.000701 0.000714 0.017887 0.002931
Nucleolus 0.000172 0.000014 0.000101 0.000157 0.000018 0.000092 0.000055 0.000122 0.000077 0.000085 0.000079 0.000141
Nucleus 0.003060 0.001690 0.015962 0.005678 0.001430 0.002615 0.007526 0.004374 0.002056 0.002532 0.011936 0.005067
Peroxisomes 0.000153 0.000202 0.000140 0.000483 0.000109 0.000303 0.000364 0.000420 0.000126 0.000294 0.000247 0.000453
Vacuole 0.009300 0.010389 0.416517* 0.160355 0.004591 0.006085 0.146690 0.109577 0.006398 0.006474 0.287736* 0.136566
Vacuole Periphery 0.012221 0.006837 0.023333 0.012666 0.006762 0.004455 0.006733 0.009426 0.008858 0.004670 0.015410 0.011148

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.87 -7.39 -6.58 1.74 5.17 -1.72 -7.79 -3.43 -2.94 4.92 -2.66 -10.23 -5.21 -3.64 6.60
Bud Neck -3.52 -12.05 -14.48 -7.73 10.13 -3.24 -10.37 -13.40 -12.95 7.62 -3.91 -14.81 -19.61 -18.57 11.99
Bud Site -3.64 -3.18 -6.93 0.63 -2.15 0.74 -7.50 -6.80 -7.19 4.33 -0.16 -8.07 -10.07 -9.44 2.30
Cell Periphery -2.72 -7.85 -9.55 -1.43 6.79 3.88 -5.54 -6.49 -6.77 1.07 1.13 -9.51 -8.46 -8.60 5.91
Cytoplasm 3.94 24.66 18.85 6.59 -15.66 0.81 15.04 15.63 14.89 -8.00 0.88 26.56 25.09 22.85 -15.88
Cytoplasmic Foci -3.51 3.87 9.20 7.20 2.69 0.31 -2.43 11.45 11.42 8.14 -0.38 0.79 14.40 13.04 7.74
Eisosomes -2.43 -8.83 -5.24 0.04 7.15 -0.88 -9.53 -5.00 -4.10 7.44 -1.74 -13.02 -7.58 -5.02 10.33
Endoplasmic Reticulum -1.45 2.10 4.14 2.10 2.21 2.67 -1.53 3.06 0.82 2.81 2.05 -0.71 4.64 2.32 3.24
Endosome -2.85 -9.33 -6.23 -0.69 4.89 -3.78 -9.63 -5.21 -2.77 6.23 -3.60 -13.48 -8.54 -4.42 7.89
Golgi -4.27 -6.74 -1.72 2.79 5.57 -2.46 -4.71 2.12 3.77 5.45 -3.63 -8.07 -0.30 2.72 7.47
Lipid Particles -4.14 -11.30 -9.33 -6.25 7.45 -0.86 -8.84 -9.01 -8.76 5.42 -2.58 -14.10 -12.87 -11.91 9.03
Mitochondria 0.25 3.93 -1.23 -1.22 -1.92 -1.80 7.13 -0.67 1.06 -2.09 -1.88 7.74 -1.30 0.95 -2.82
Mitotic Spindle -2.76 -6.25 -3.95 -1.66 1.70 -2.76 -4.91 -2.78 -0.56 3.26 -3.27 -7.91 -4.72 -2.40 3.28
None 2.84 2.49 2.62 -0.89 0.61 -1.42 5.32 -0.08 0.57 -0.93 0.04 3.81 1.53 1.32 -0.79
Nuclear Periphery -0.09 -8.61 -2.94 -2.63 7.90 -1.08 -9.83 -2.35 -2.28 2.11 1.14 -9.33 -3.90 -4.19 7.79
Nuclear Periphery Foci -2.04 -9.62 -4.74 -2.99 8.45 -0.78 -11.40 -11.23 -8.05 9.01 -0.12 -13.40 -9.18 -7.79 11.29
Nucleolus 1.65 0.62 0.15 -13.43 -3.28 -1.27 -6.48 -10.53 -0.67 -5.81 -0.10 -0.26 -1.78 -1.16 -6.18
Nucleus 3.22 -6.70 -6.33 -9.44 4.89 -2.81 -10.71 -10.77 -5.79 3.47 -1.19 -9.02 -11.74 -8.14 5.50
Peroxisomes -0.82 -0.15 -5.06 -5.93 -7.16 -2.21 -5.29 -8.10 -1.08 -0.69 -2.04 -4.10 -9.72 -1.67 -4.84
Vacuole -0.33 -18.73 -25.01 -22.55 10.63 -2.16 -10.57 -21.08 -20.81 2.27 -0.15 -19.51 -32.60 -32.57 9.32
Vacuole Periphery 1.99 -2.39 0.56 -1.81 2.99 2.93 0.30 -1.46 -3.66 -1.30 4.97 -2.52 -1.09 -6.41 1.87
Standard name
Human Ortholog
Description Translational repressor with a role in polar growth and wall integrity; regulated by Cbk1p phosphorylation to effect bud-specific translational control and localization of specific mRNAs; interacts with TOR pathway components; contains a functional N-terminal nuclear localization sequence and nucleocytoplasmic shuttling appears to be critical to Ssd1p function
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ssd1

Ssd1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssd1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available