Standard name
Human Ortholog
Description Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.93 0.95 0.93 0.95 0.85 0.77 0.63 0.45 0.44 0.36 0.38 0.99 1.0 0.99 0.92 0.95 0.9 0.95 0.81 0.71 0.86 0.8 0.77
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.1 0 0 0 0.07 0.15 0.21 0.64 0.51 0.64 0.59 0 0 0 0 0 0 0 0.07 0.15 0.07 0.07 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0.09 0.12 0.18 0.1 0.11 0.09 0.15 0 0 0 0.06 0.05 0.12 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 1 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 2 3 0 3 4
Bud 1 2 0 0 0 0 0 4 7 9 5 3 0 0 0 0 0 0 0 0 1 0 1 2
Bud Neck 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 1
Bud Site 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 2 1 1 1 3 4 0 5 2 2 0 1 2 2 5 1 0 0 1 0 1 0
Cytoplasm 140 95 167 88 156 137 165 183 99 104 64 73 114 162 218 93 106 103 141 90 144 129 188 209
Endoplasmic Reticulum 1 0 1 1 3 4 2 1 1 2 2 4 0 0 0 6 3 2 0 0 3 0 1 3
Endosome 0 0 2 0 2 0 3 12 3 8 4 1 0 0 1 1 0 0 2 2 4 1 5 5
Golgi 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Mitochondria 1 10 0 4 1 12 32 60 141 121 112 113 4 0 0 4 3 1 1 8 30 10 16 22
Nucleus 0 0 2 2 0 3 7 5 3 3 7 6 0 0 0 1 0 1 0 0 0 0 1 1
Nuclear Periphery 0 0 0 0 0 0 0 1 0 3 1 1 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 3 0 3 0 1 0 0 0 0 0 0 0 0 2 1 2 6
Vac/Vac Membrane 1 1 2 5 6 15 25 51 22 27 16 28 1 1 2 6 6 14 2 2 3 2 2 4
Unique Cell Count 142 102 176 95 165 162 215 290 220 239 176 193 115 162 220 101 112 115 149 111 203 150 235 272
Labelled Cell Count 145 109 178 102 169 172 238 326 278 288 213 234 119 164 224 114 123 122 149 111 203 150 235 272


Cytoplasm, Mitochondria, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.6 4.2 6.2 6.1 6.7 5.8 5.7 5.9 4.7 5.1 5.3 5.2 5.9 6.7 6.2 6.7 8.1 8.7 6.6 7.1 6.7
Std Deviation (1e-4) 0.8 0.8 1.1 1.4 1.5 1.1 1.3 1.4 1.3 1.1 1.6 1.2 1.1 1.7 1.3 1.8 1.5 1.8 1.3 1.9 1.5
Intensity Change (Log2) – – – -0.02 0.11 -0.11 -0.12 -0.08 -0.4 -0.28 -0.24 -0.26 -0.08 0.11 -0.01 0.11 0.38 0.48 0.09 0.19 0.11


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – 0
Bud Neck – – – – – – – – – – – – – – 0
Bud Site – – – – – – – – – – – – – – 0
Cell Periphery – – – – – – – – – – – – – – 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – 0
Endosome – – – – – – – – – – – – – – 0
Golgi – – – – – – – – – – – – – – 0
Mitochondria – – – – – – – – – – – – – – 0
Nuclear Periphery – – – – – – – – – – – – – – 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – 0
Peroxisomes – – – – – – – – – – – – – – 0
SpindlePole – – – – – – – – – – – – – – 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – 0
Cytoplasm – – – – – – – – – – – – – – 0
Nucleus – – – – – – – – – – – – – – 0
Vacuole – – – – – – – – – – – – – – 4.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.3016 2.8958 1.8413 1.6271 1.5332 2.2522 4.9141 6.1237 5.1199 5.4499 4.8271 5.0776 1.6664 2.7074 2.1986 2.1539 2.3191 2.3519
Actin 0.0022 0.001 0.0048 0.0004 0.0011 0.0023 0.0287 0.0136 0.0131 0.0184 0.0019 0.0016 0.0141 0.0008 0.0084 0.0031 0.0037 0.004
Bud 0.0008 0.0043 0.0011 0.0021 0.0056 0.0018 0.001 0.0004 0.001 0.0009 0.0047 0.0001 0.0003 0.0004 0.0068 0.001 0.0004 0.0002
Bud Neck 0.0009 0.0003 0.0006 0.0005 0.0006 0.0019 0.0007 0.0003 0.0026 0.007 0.004 0.0045 0.0009 0.0003 0.0007 0.0005 0.0006 0.0017
Bud Periphery 0.001 0.0004 0.0013 0.0019 0.017 0.0006 0.0028 0.0003 0.0013 0.0013 0.0056 0.0001 0.0003 0.0002 0.0031 0.0014 0.0003 0.0001
Bud Site 0.0057 0.0061 0.0023 0.0006 0.0008 0.0003 0.0034 0.0061 0.0028 0.0043 0.0013 0.0002 0.0012 0.0029 0.0055 0.0008 0.0007 0.0002
Cell Periphery 0.0004 0.0002 0.0001 0.0001 0.0003 0 0.0005 0.0006 0.001 0.0005 0.0003 0.0001 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001
Cytoplasm 0.7373 0.8388 0.7662 0.8764 0.7668 0.8311 0.5365 0.798 0.7251 0.7089 0.8298 0.8212 0.6566 0.8955 0.7596 0.7356 0.7981 0.8402
Cytoplasmic Foci 0.0378 0.0217 0.0272 0.019 0.0274 0.0375 0.0451 0.0107 0.0321 0.0503 0.0186 0.034 0.0364 0.0226 0.0156 0.0282 0.0253 0.0397
Eisosomes 0.0001 0.0001 0.0001 0 0 0 0.0005 0.0001 0.0003 0.0003 0 0.0001 0.0002 0 0.0001 0.0004 0.0001 0
Endoplasmic Reticulum 0.0076 0.0049 0.0045 0.0038 0.006 0.0051 0.0102 0.0039 0.0092 0.0033 0.0059 0.0025 0.0122 0.0018 0.0045 0.0056 0.0067 0.0029
Endosome 0.0348 0.0174 0.0173 0.0201 0.0513 0.0237 0.0445 0.0049 0.0251 0.0113 0.0251 0.0114 0.0337 0.0082 0.0128 0.0187 0.0151 0.0135
Golgi 0.0021 0.0011 0.0021 0.0012 0.0035 0.0014 0.0077 0.0014 0.0112 0.006 0.0028 0.0012 0.0048 0.0014 0.0025 0.0026 0.0027 0.0018
Lipid Particles 0.0009 0.0004 0.0012 0.0005 0.0001 0.0004 0.0146 0.0004 0.0053 0.0135 0.0043 0.0001 0.0058 0.0001 0.0006 0.0015 0.0005 0.0015
Mitochondria 0.0038 0.0007 0.005 0.0014 0.008 0.0015 0.0121 0.0004 0.006 0.0023 0.0062 0.0015 0.0033 0.0009 0.0033 0.0025 0.0016 0.0011
None 0.1421 0.0873 0.1521 0.0604 0.0948 0.0708 0.2491 0.1492 0.1445 0.1436 0.0679 0.1169 0.1964 0.0591 0.1659 0.1793 0.1261 0.084
Nuclear Periphery 0.0053 0.0021 0.002 0.0018 0.002 0.0019 0.0034 0.0005 0.0019 0.001 0.0053 0.0003 0.012 0.0005 0.0012 0.0017 0.0059 0.0007
Nucleolus 0.0004 0.0002 0.0002 0.0001 0.0001 0.0001 0.0006 0.0001 0.0002 0.0004 0.0002 0 0.0006 0 0.0001 0.0003 0.0001 0.0001
Nucleus 0.007 0.0048 0.0051 0.0042 0.0066 0.0122 0.0061 0.0012 0.0015 0.0078 0.0093 0.0016 0.0064 0.0019 0.0024 0.0031 0.0059 0.0026
Peroxisomes 0.0009 0.0012 0.0017 0.0006 0.0007 0.0008 0.0214 0.0033 0.0057 0.015 0.0009 0.0014 0.0042 0.001 0.0008 0.003 0.0018 0.0026
Punctate Nuclear 0.0035 0.0012 0.0017 0.0018 0.001 0.0036 0.0056 0.0003 0.0008 0.0023 0.0013 0.0005 0.006 0.0007 0.0041 0.0009 0.003 0.0016
Vacuole 0.0045 0.005 0.0027 0.0025 0.005 0.0024 0.0044 0.004 0.0082 0.0013 0.0034 0.0006 0.0034 0.0012 0.0015 0.0085 0.0011 0.0011
Vacuole Periphery 0.0011 0.0007 0.0006 0.0005 0.0014 0.0006 0.0012 0.0002 0.0011 0.0003 0.0012 0.0002 0.0013 0.0002 0.0003 0.0009 0.0003 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.1845 9.0493 10.0725 16.7492 12.0311 21.921 19.8245 21.0995 15.8491 15.8765
Translational Efficiency 1.0434 1.7237 1.7469 0.9311 1.2968 0.9214 1.0448 0.848 1.38 1.2659

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1222 825 2791 1786 1240 1956 1234 1971 2462 2781 4025 3757

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 700.29 818.23 917.70 888.24 710.88 873.38 903.16 902.30 705.62 857.02 913.24 895.62
Standard Deviation 91.17 102.81 107.62 121.63 92.22 97.22 126.74 122.58 91.85 102.07 114.02 122.33
Intensity Change Log 2 — 0.224554 0.390070 0.342997 — 0.297003 0.345376 0.344001 — 0.261505 0.367728 0.343503

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000411 0.000703 0.000209 0.003189 0.000275 0.000635 0.000168 0.000732 0.000342 0.000655 0.000197 0.001900
Bud Neck 0.028190 0.022318 0.006878 0.012446 0.006748 0.028811 0.002728 0.007589 0.017391 0.026885 0.005605 0.009898
Bud Site 0.016645 0.026421 0.012736 0.047306 0.008191 0.015666 0.003498 0.033966 0.012387 0.018857 0.009904 0.040308
Cell Periphery 0.000268 0.000148 0.000059 0.000158 0.000139 0.000135 0.000158 0.000132 0.000203 0.000139 0.000090 0.000144
Cytoplasm 0.344823* 0.422193* 0.655492* 0.449567* 0.602694* 0.466041* 0.507697* 0.625613* 0.474701* 0.453033* 0.610181* 0.541924*
Cytoplasmic Foci 0.322298* 0.245738* 0.017410 0.042969 0.129674 0.221783* 0.025863 0.047100 0.225282* 0.228889* 0.020002 0.045136
Eisosomes 0.000210 0.000091 0.000018 0.000057 0.000044 0.000115 0.000054 0.000041 0.000126 0.000108 0.000029 0.000049
Endoplasmic Reticulum 0.002636 0.002159 0.001357 0.002798 0.002168 0.001184 0.002358 0.001484 0.002400 0.001473 0.001664 0.002109
Endosome 0.027855 0.024362 0.002346 0.013203 0.007941 0.018482 0.004418 0.004430 0.017825 0.020226 0.002982 0.008601
Golgi 0.010232 0.008688 0.000172 0.009271 0.001647 0.005477 0.000114 0.000524 0.005908 0.006430 0.000154 0.004682
Lipid Particles 0.014270 0.005240 0.000783 0.001484 0.003317 0.004742 0.002173 0.001331 0.008753 0.004890 0.001209 0.001404
Mitochondria 0.024580 0.008604 0.001658 0.006259 0.005509 0.009471 0.000599 0.000817 0.014974 0.009214 0.001333 0.003404
Mitotic Spindle 0.003637 0.010327 0.021391 0.071217 0.002401 0.006191 0.016371 0.017202 0.003014 0.007418 0.019852 0.042879
None 0.002765 0.001678 0.004017 0.003435 0.006361 0.001455 0.010741 0.006498 0.004576 0.001521 0.006078 0.005042
Nuclear Periphery 0.000606 0.000490 0.001664 0.001536 0.000460 0.000443 0.001388 0.000765 0.000533 0.000457 0.001579 0.001132
Nuclear Periphery Foci 0.000534 0.000958 0.000668 0.001192 0.001145 0.000306 0.001854 0.001440 0.000842 0.000499 0.001031 0.001322
Nucleolus 0.001744 0.001489 0.000407 0.000568 0.001456 0.001081 0.000820 0.000611 0.001599 0.001202 0.000534 0.000590
Nucleus 0.126763 0.115384 0.187832* 0.155167* 0.176134* 0.134402* 0.320484* 0.130320* 0.151629* 0.128760 0.228501* 0.142131*
Peroxisomes 0.015818 0.015402 0.000594 0.004264 0.002173 0.009453 0.000495 0.003990 0.008946 0.011218 0.000564 0.004120
Vacuole 0.051780 0.084568 0.083905 0.168089 0.039148 0.071555 0.097749 0.114717 0.045418 0.075416 0.088149 0.140089
Vacuole Periphery 0.003937 0.003039 0.000403 0.005822 0.002374 0.002574 0.000269 0.000698 0.003150 0.002712 0.000362 0.003134

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.16 3.79 -3.35 -2.29 -3.93 -1.37 0.11 -5.35 -0.48 -5.97 -1.66 2.91 -4.75 -1.90 -5.43
Bud Neck 2.19 10.84 9.48 6.02 -2.51 -12.05 4.10 -1.44 11.63 -6.99 -5.66 10.44 7.80 12.97 -4.53
Bud Site -3.09 3.68 -3.23 0.29 -6.54 -5.00 3.85 -7.43 -3.75 -9.52 -4.32 3.70 -7.29 -3.27 -10.16
Cell Periphery 4.00 7.21 5.44 3.65 -8.65 0.49 -0.77 3.07 2.16 3.67 3.97 7.64 6.10 3.50 -4.63
Cytoplasm -5.79 -23.92 -4.09 2.69 21.99 13.68 14.68 5.08 -9.63 -11.06 2.74 -7.42 0.12 -2.97 8.76
Cytoplasmic Foci 7.83 44.76 42.20 27.88 -8.30 -14.17 24.01 17.81 34.01 -10.79 -0.44 45.99 40.55 44.82 -14.80
Eisosomes 10.48 17.79 14.32 7.64 -16.35 -9.45 -4.26 -1.43 8.99 3.25 2.50 16.44 13.05 10.35 -11.04
Endoplasmic Reticulum 1.04 1.62 -0.44 -2.47 -4.20 3.79 -4.83 2.05 -2.42 7.32 3.58 -0.18 0.65 -4.19 1.42
Endosome 1.41 12.94 10.41 8.77 -6.25 -8.13 4.94 5.70 12.91 0.38 -1.59 14.12 11.61 14.87 -5.98
Golgi 1.21 11.46 6.04 3.53 -4.30 -5.49 5.46 4.36 7.95 -4.12 -0.56 12.36 7.07 6.72 -4.66
Lipid Particles 7.78 12.94 12.27 6.30 -9.98 -3.43 3.23 6.47 10.67 2.26 6.44 13.61 13.31 12.20 -2.44
Mitochondria 5.96 9.59 8.86 3.97 -2.97 -2.63 4.52 4.38 8.26 -1.28 3.75 10.32 9.82 8.53 -2.22
Mitotic Spindle -2.91 -9.73 -10.61 -7.57 -4.25 -2.93 -5.36 -7.16 -4.30 1.11 -3.81 -11.38 -12.67 -9.28 -1.94
None 2.66 -2.12 -0.85 -3.83 1.20 7.60 -1.41 1.50 -4.14 2.38 7.90 -1.03 0.88 -5.44 1.77
Nuclear Periphery 1.65 -20.75 -15.34 -16.64 0.42 0.17 -16.81 -9.55 -10.44 10.22 1.51 -26.70 -17.74 -19.20 7.49
Nuclear Periphery Foci -4.19 -4.67 -8.87 -2.77 -4.44 7.77 -4.04 -2.18 -8.30 2.30 5.30 -4.17 -5.46 -10.13 -1.22
Nucleolus 1.20 4.78 4.52 4.98 -1.32 1.85 3.80 4.10 2.55 0.15 2.42 6.53 6.00 4.98 -1.10
Nucleus 1.16 -15.64 -9.94 -10.65 5.12 6.00 -16.72 3.76 -3.06 22.14 4.42 -20.37 -4.19 -9.80 18.73
Peroxisomes 0.39 12.56 10.84 8.28 -4.36 -10.70 6.96 -2.07 8.13 -6.45 -2.39 13.38 9.08 12.11 -7.66
Vacuole -7.38 -17.88 -33.34 -24.55 -21.24 -9.65 -18.03 -26.89 -19.97 -4.31 -11.30 -26.29 -42.00 -32.74 -18.99
Vacuole Periphery 1.90 10.00 2.82 1.26 -3.56 -0.45 7.72 6.04 5.92 -1.49 1.40 12.44 5.45 3.91 -3.76
Standard name
Human Ortholog
Description Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins
Localization
Cell Percentages cytoplasm (44%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Hnt2

Hnt2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hnt2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available