Standard name | |
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Human Ortholog | |
Description | Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 3 | 4 |
Bud | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 4 | 7 | 9 | 5 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 2 | 1 | 1 | 1 | 3 | 4 | 0 | 5 | 2 | 2 | 0 | 1 | 2 | 2 | 5 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 140 | 95 | 167 | 88 | 156 | 137 | 165 | 183 | 99 | 104 | 64 | 73 | 114 | 162 | 218 | 93 | 106 | 103 | 141 | 90 | 144 | 129 | 188 | 209 |
Endoplasmic Reticulum | 1 | 0 | 1 | 1 | 3 | 4 | 2 | 1 | 1 | 2 | 2 | 4 | 0 | 0 | 0 | 6 | 3 | 2 | 0 | 0 | 3 | 0 | 1 | 3 |
Endosome | 0 | 0 | 2 | 0 | 2 | 0 | 3 | 12 | 3 | 8 | 4 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 2 | 4 | 1 | 5 | 5 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 1 | 10 | 0 | 4 | 1 | 12 | 32 | 60 | 141 | 121 | 112 | 113 | 4 | 0 | 0 | 4 | 3 | 1 | 1 | 8 | 30 | 10 | 16 | 22 |
Nucleus | 0 | 0 | 2 | 2 | 0 | 3 | 7 | 5 | 3 | 3 | 7 | 6 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 3 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 6 |
Vac/Vac Membrane | 1 | 1 | 2 | 5 | 6 | 15 | 25 | 51 | 22 | 27 | 16 | 28 | 1 | 1 | 2 | 6 | 6 | 14 | 2 | 2 | 3 | 2 | 2 | 4 |
Unique Cell Count | 142 | 102 | 176 | 95 | 165 | 162 | 215 | 290 | 220 | 239 | 176 | 193 | 115 | 162 | 220 | 101 | 112 | 115 | 149 | 111 | 203 | 150 | 235 | 272 |
Labelled Cell Count | 145 | 109 | 178 | 102 | 169 | 172 | 238 | 326 | 278 | 288 | 213 | 234 | 119 | 164 | 224 | 114 | 123 | 122 | 149 | 111 | 203 | 150 | 235 | 272 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.6 | 4.2 | 6.2 | 6.1 | 6.7 | 5.8 | 5.7 | 5.9 | 4.7 | 5.1 | 5.3 | 5.2 | 5.9 | 6.7 | 6.2 | 6.7 | 8.1 | 8.7 | 6.6 | 7.1 | 6.7 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.1 | 1.4 | 1.5 | 1.1 | 1.3 | 1.4 | 1.3 | 1.1 | 1.6 | 1.2 | 1.1 | 1.7 | 1.3 | 1.8 | 1.5 | 1.8 | 1.3 | 1.9 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.11 | -0.11 | -0.12 | -0.08 | -0.4 | -0.28 | -0.24 | -0.26 | -0.08 | 0.11 | -0.01 | 0.11 | 0.38 | 0.48 | 0.09 | 0.19 | 0.11 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 4.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3016 | 2.8958 | 1.8413 | 1.6271 | 1.5332 | 2.2522 | 4.9141 | 6.1237 | 5.1199 | 5.4499 | 4.8271 | 5.0776 | 1.6664 | 2.7074 | 2.1986 | 2.1539 | 2.3191 | 2.3519 |
Actin | 0.0022 | 0.001 | 0.0048 | 0.0004 | 0.0011 | 0.0023 | 0.0287 | 0.0136 | 0.0131 | 0.0184 | 0.0019 | 0.0016 | 0.0141 | 0.0008 | 0.0084 | 0.0031 | 0.0037 | 0.004 |
Bud | 0.0008 | 0.0043 | 0.0011 | 0.0021 | 0.0056 | 0.0018 | 0.001 | 0.0004 | 0.001 | 0.0009 | 0.0047 | 0.0001 | 0.0003 | 0.0004 | 0.0068 | 0.001 | 0.0004 | 0.0002 |
Bud Neck | 0.0009 | 0.0003 | 0.0006 | 0.0005 | 0.0006 | 0.0019 | 0.0007 | 0.0003 | 0.0026 | 0.007 | 0.004 | 0.0045 | 0.0009 | 0.0003 | 0.0007 | 0.0005 | 0.0006 | 0.0017 |
Bud Periphery | 0.001 | 0.0004 | 0.0013 | 0.0019 | 0.017 | 0.0006 | 0.0028 | 0.0003 | 0.0013 | 0.0013 | 0.0056 | 0.0001 | 0.0003 | 0.0002 | 0.0031 | 0.0014 | 0.0003 | 0.0001 |
Bud Site | 0.0057 | 0.0061 | 0.0023 | 0.0006 | 0.0008 | 0.0003 | 0.0034 | 0.0061 | 0.0028 | 0.0043 | 0.0013 | 0.0002 | 0.0012 | 0.0029 | 0.0055 | 0.0008 | 0.0007 | 0.0002 |
Cell Periphery | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0006 | 0.001 | 0.0005 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 |
Cytoplasm | 0.7373 | 0.8388 | 0.7662 | 0.8764 | 0.7668 | 0.8311 | 0.5365 | 0.798 | 0.7251 | 0.7089 | 0.8298 | 0.8212 | 0.6566 | 0.8955 | 0.7596 | 0.7356 | 0.7981 | 0.8402 |
Cytoplasmic Foci | 0.0378 | 0.0217 | 0.0272 | 0.019 | 0.0274 | 0.0375 | 0.0451 | 0.0107 | 0.0321 | 0.0503 | 0.0186 | 0.034 | 0.0364 | 0.0226 | 0.0156 | 0.0282 | 0.0253 | 0.0397 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0076 | 0.0049 | 0.0045 | 0.0038 | 0.006 | 0.0051 | 0.0102 | 0.0039 | 0.0092 | 0.0033 | 0.0059 | 0.0025 | 0.0122 | 0.0018 | 0.0045 | 0.0056 | 0.0067 | 0.0029 |
Endosome | 0.0348 | 0.0174 | 0.0173 | 0.0201 | 0.0513 | 0.0237 | 0.0445 | 0.0049 | 0.0251 | 0.0113 | 0.0251 | 0.0114 | 0.0337 | 0.0082 | 0.0128 | 0.0187 | 0.0151 | 0.0135 |
Golgi | 0.0021 | 0.0011 | 0.0021 | 0.0012 | 0.0035 | 0.0014 | 0.0077 | 0.0014 | 0.0112 | 0.006 | 0.0028 | 0.0012 | 0.0048 | 0.0014 | 0.0025 | 0.0026 | 0.0027 | 0.0018 |
Lipid Particles | 0.0009 | 0.0004 | 0.0012 | 0.0005 | 0.0001 | 0.0004 | 0.0146 | 0.0004 | 0.0053 | 0.0135 | 0.0043 | 0.0001 | 0.0058 | 0.0001 | 0.0006 | 0.0015 | 0.0005 | 0.0015 |
Mitochondria | 0.0038 | 0.0007 | 0.005 | 0.0014 | 0.008 | 0.0015 | 0.0121 | 0.0004 | 0.006 | 0.0023 | 0.0062 | 0.0015 | 0.0033 | 0.0009 | 0.0033 | 0.0025 | 0.0016 | 0.0011 |
None | 0.1421 | 0.0873 | 0.1521 | 0.0604 | 0.0948 | 0.0708 | 0.2491 | 0.1492 | 0.1445 | 0.1436 | 0.0679 | 0.1169 | 0.1964 | 0.0591 | 0.1659 | 0.1793 | 0.1261 | 0.084 |
Nuclear Periphery | 0.0053 | 0.0021 | 0.002 | 0.0018 | 0.002 | 0.0019 | 0.0034 | 0.0005 | 0.0019 | 0.001 | 0.0053 | 0.0003 | 0.012 | 0.0005 | 0.0012 | 0.0017 | 0.0059 | 0.0007 |
Nucleolus | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0 | 0.0006 | 0 | 0.0001 | 0.0003 | 0.0001 | 0.0001 |
Nucleus | 0.007 | 0.0048 | 0.0051 | 0.0042 | 0.0066 | 0.0122 | 0.0061 | 0.0012 | 0.0015 | 0.0078 | 0.0093 | 0.0016 | 0.0064 | 0.0019 | 0.0024 | 0.0031 | 0.0059 | 0.0026 |
Peroxisomes | 0.0009 | 0.0012 | 0.0017 | 0.0006 | 0.0007 | 0.0008 | 0.0214 | 0.0033 | 0.0057 | 0.015 | 0.0009 | 0.0014 | 0.0042 | 0.001 | 0.0008 | 0.003 | 0.0018 | 0.0026 |
Punctate Nuclear | 0.0035 | 0.0012 | 0.0017 | 0.0018 | 0.001 | 0.0036 | 0.0056 | 0.0003 | 0.0008 | 0.0023 | 0.0013 | 0.0005 | 0.006 | 0.0007 | 0.0041 | 0.0009 | 0.003 | 0.0016 |
Vacuole | 0.0045 | 0.005 | 0.0027 | 0.0025 | 0.005 | 0.0024 | 0.0044 | 0.004 | 0.0082 | 0.0013 | 0.0034 | 0.0006 | 0.0034 | 0.0012 | 0.0015 | 0.0085 | 0.0011 | 0.0011 |
Vacuole Periphery | 0.0011 | 0.0007 | 0.0006 | 0.0005 | 0.0014 | 0.0006 | 0.0012 | 0.0002 | 0.0011 | 0.0003 | 0.0012 | 0.0002 | 0.0013 | 0.0002 | 0.0003 | 0.0009 | 0.0003 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.1845 | 9.0493 | 10.0725 | 16.7492 | 12.0311 | 21.921 | 19.8245 | 21.0995 | 15.8491 | 15.8765 |
Translational Efficiency | 1.0434 | 1.7237 | 1.7469 | 0.9311 | 1.2968 | 0.9214 | 1.0448 | 0.848 | 1.38 | 1.2659 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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