Standard name
Human Ortholog
Description Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0.07 0.05 0 0 – 0.05 0.06 0.05 0.06 0 0 0 0.13 0.15 0.14 0 0 0 0.05 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0 0 – 0 0 0 0.05 0 0.06 0.1 0.23 0.19 0.19 0 0 0 0 0 0
Cytoplasm 0.07 0.09 0.24 0.16 0.25 0.23 0.24 – 0.17 0.25 0.2 0.16 0.17 0.25 0.17 0.46 0.45 0.51 0 0 0 0 0.09 0.24
Endoplasmic Reticulum 0.9 0.83 0.65 0.69 0.29 0.55 0.52 – 0.62 0.45 0.41 0.51 0.76 0.69 0.77 0 0 0 0.91 0.9 0.68 0.74 0.73 0.56
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.09 0.13 0.16 – 0.17 0.21 0.51 0.32 0 0 0 0 0.06 0.07 0 0 0.08 0.08 0.08 0.05
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.09 0 0 0 0 0 0 – 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.12 0 0.16 0.39 0.17 0.18 – 0.06 0.13 0.06 0.08 0 0.08 0.06 0.15 0.17 0.15 0 0 0 0.05 0 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 9 2 9 14 11 8 – 12 15 8 14 2 3 3 24 24 18 1 1 4 1 1 2
Bud 0 0 1 1 4 2 1 – 5 3 4 7 1 1 0 0 1 1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 6 2 2 8 5 9 – 10 11 6 13 2 6 11 41 30 25 0 0 0 0 0 0
Cytoplasm 8 24 9 20 67 56 78 – 42 62 31 37 12 26 20 83 73 67 2 5 2 1 7 36
Endoplasmic Reticulum 96 219 24 88 78 136 168 – 154 112 64 120 55 73 89 5 6 2 118 248 62 27 60 85
Endosome 2 0 0 1 9 5 7 – 0 1 0 1 0 1 0 0 6 1 0 1 1 0 0 0
Golgi 0 2 0 0 6 0 0 – 0 2 0 0 0 0 0 8 5 2 0 2 1 0 0 1
Mitochondria 2 2 0 2 23 33 51 – 43 53 81 75 1 1 0 8 9 9 1 3 7 2 6 7
Nucleus 0 1 1 3 0 2 6 – 2 1 1 2 1 1 0 1 1 2 0 0 0 0 0 0
Nuclear Periphery 10 7 0 5 1 2 0 – 2 5 7 6 5 1 2 2 0 0 1 4 2 0 1 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Vac/Vac Membrane 7 32 1 20 103 41 59 – 15 33 10 18 2 8 7 27 28 20 1 7 3 1 1 14
Unique Cell Count 107 265 37 127 266 246 324 250 247 158 237 72 106 115 180 161 132 130 277 93 37 83 152
Labelled Cell Count 130 302 40 151 314 293 387 285 298 212 293 81 123 132 200 183 148 130 277 93 37 83 152


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 9.8 7.7 8.0 8.6 7.5 7.5 – 6.6 6.9 5.9 6.5 7.9 8.7 8.8 9.5 9.0 8.7 – 6.1 7.8
Std Deviation (1e-4) 1.0 1.4 1.1 1.3 1.6 1.3 1.6 – 1.0 1.2 1.1 1.1 1.4 1.6 1.2 1.6 1.5 1.6 – 0.7 1.1
Intensity Change (Log2) – – – 0.06 0.17 -0.04 -0.03 – -0.21 -0.16 -0.38 -0.23 0.05 0.18 0.19 0.31 0.23 0.18 – -0.32 0.02


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 2.4 2.0 2.0
Cytoplasm -1.2 0.1 -0.2 0 – -1.1 0.1 -0.6 -1.3 -1.0 0 -0.9 2.4 2.3 2.9
Endoplasmic Reticulum 0.5 -4.3 -1.1 -1.5 – -0.4 -2.2 -2.7 -1.6 1.3 0.4 1.5 0 -9.4 -9.4
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.6 – 2.7 3.1 5.7 4.0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.3 2.2 2.4 – 0 0 0 0 0 0 0 0 2.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.4017 1.9234 1.6806 1.4826 1.4019 1.8988 2.0435 3.0134 2.6855 2.397 2.208 2.6328 1.1634 1.8519 1.4687 1.7698 0.7903 1.5063
Actin 0.0086 0.0015 0.02 0.0063 0.0005 0.0151 0.0136 0.0027 0.0039 0.0434 0.0006 0.0008 0.0128 0.0063 0.0018 0.0489 0.0235 0.0008
Bud 0 0.0001 0.0004 0.0004 0 0 0.0003 0.0002 0.0017 0.0002 0 0 0.0001 0.0011 0.0002 0.0003 0.0003 0
Bud Neck 0.0004 0.0003 0.0006 0.0012 0.0002 0.0008 0.0025 0.0003 0.0008 0.0032 0.0003 0.0008 0.0003 0.0007 0.0005 0.0007 0.0023 0.0009
Bud Periphery 0.0001 0.0001 0.0005 0.0008 0.0001 0.0001 0.0006 0.0002 0.0019 0.0003 0 0.0001 0.0002 0.0005 0.0003 0.0004 0.0004 0.0001
Bud Site 0.0005 0.003 0.0046 0.0007 0.0001 0.0001 0.008 0.0043 0.0052 0.0005 0.0001 0.0001 0.0004 0.0119 0.0029 0.001 0.0018 0.0001
Cell Periphery 0.0006 0.0015 0.002 0.0013 0.0003 0.0003 0.0018 0.0017 0.0014 0.0006 0.0002 0.0004 0.0008 0.0038 0.0011 0.0005 0.0004 0.0003
Cytoplasm 0.0897 0.1629 0.1103 0.088 0.0068 0.1224 0.0882 0.156 0.0781 0.0752 0.1478 0.1334 0.1057 0.2098 0.1465 0.0831 0.2034 0.1723
Cytoplasmic Foci 0.0083 0.0111 0.0152 0.0505 0.0112 0.0072 0.0221 0.0119 0.0131 0.0081 0.0025 0.0068 0.0101 0.0134 0.0077 0.023 0.0342 0.0062
Eisosomes 0.0001 0.0002 0.0015 0.0001 0 0.0001 0.0005 0.0001 0.0001 0.0002 0 0 0.0005 0.0007 0.0001 0.0003 0.0003 0
Endoplasmic Reticulum 0.7543 0.6351 0.6606 0.5693 0.8141 0.7557 0.6294 0.7111 0.7608 0.7252 0.7047 0.7664 0.6328 0.5893 0.6814 0.6992 0.446 0.6817
Endosome 0.0318 0.0255 0.0391 0.0661 0.0325 0.0207 0.0565 0.0209 0.0207 0.0348 0.0101 0.0174 0.0256 0.0302 0.0327 0.0477 0.08 0.031
Golgi 0.004 0.0027 0.0175 0.0529 0.0062 0.0144 0.0086 0.0026 0.0074 0.0136 0.0004 0.002 0.0045 0.0054 0.0023 0.0115 0.022 0.0009
Lipid Particles 0.0184 0.033 0.0237 0.0386 0.0577 0.01 0.0225 0.0091 0.0207 0.0206 0.0015 0.0074 0.0143 0.0089 0.0066 0.0152 0.0355 0.006
Mitochondria 0.0008 0.0006 0.0144 0.0138 0.0067 0.0011 0.0108 0.0009 0.017 0.0143 0.0002 0.0018 0.0025 0.0042 0.0031 0.0032 0.0052 0.0012
None 0.0386 0.0797 0.0344 0.0079 0.0007 0.0107 0.0662 0.0413 0.0255 0.0035 0.1029 0.0297 0.097 0.0593 0.0554 0.0087 0.0694 0.0217
Nuclear Periphery 0.0302 0.0264 0.031 0.0429 0.0376 0.0321 0.0336 0.0156 0.0176 0.0428 0.0183 0.016 0.0667 0.0295 0.04 0.0264 0.04 0.0487
Nucleolus 0.0001 0.0002 0.0003 0.0003 0.0001 0 0.0015 0.0003 0.0005 0.0001 0.0001 0.0001 0.0006 0.0006 0.0003 0.0003 0.0006 0.0004
Nucleus 0.0022 0.0037 0.0036 0.0043 0.0011 0.003 0.0063 0.003 0.0021 0.0024 0.0053 0.0039 0.007 0.0099 0.0052 0.0066 0.0055 0.0107
Peroxisomes 0.0033 0.0002 0.0089 0.0178 0.0158 0.001 0.0087 0.0079 0.0135 0.0036 0 0.0058 0.0034 0.0005 0.0004 0.0081 0.0135 0.0001
Punctate Nuclear 0.0008 0.0034 0.003 0.0222 0.0004 0.0008 0.005 0.0014 0.0009 0.0007 0.0009 0.0011 0.0075 0.0059 0.0039 0.006 0.0072 0.0086
Vacuole 0.0058 0.0077 0.0067 0.0109 0.0047 0.0033 0.0102 0.0076 0.0055 0.0048 0.0035 0.005 0.0047 0.0068 0.0062 0.0073 0.0067 0.0062
Vacuole Periphery 0.0013 0.0009 0.0016 0.0038 0.0034 0.0009 0.0028 0.0008 0.0017 0.002 0.0005 0.0008 0.0026 0.0014 0.0016 0.0015 0.0019 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.6865 8.4306 6.6238 15.3008 14.0256 11.6434 10.7805 13.0293 13.6024 21.3828
Translational Efficiency 0.8618 0.9336 1.336 0.6975 1.0965 1.9133 1.0531 0.8124 0.7901 0.8268

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2201 913 904 1226 1181 1885 98 2453 3382 2798 1002 3679

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 791.01 863.03 1242.95 998.85 847.96 879.06 1280.55 1002.74 810.90 873.83 1246.63 1001.44
Standard Deviation 93.99 138.47 151.73 139.78 108.09 123.06 148.21 140.81 102.79 128.51 151.80 140.48
Intensity Change Log 2 — 0.125715 0.652000 0.336572 — 0.051965 0.594695 0.241879 — 0.088030 0.622637 0.288357

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000464 0.001373 0.000832 0.001130 0.000434 0.000290 0.000410 0.001047 0.000453 0.000643 0.000790 0.001075
Bud Neck 0.000495 0.001461 0.001168 0.004893 0.000470 0.000429 0.001250 0.009429 0.000486 0.000766 0.001176 0.007917
Bud Site 0.001393 0.005198 0.001306 0.009450 0.001336 0.001262 0.001220 0.014793 0.001373 0.002546 0.001298 0.013013
Cell Periphery 0.000592 0.000549 0.000242 0.000873 0.000571 0.000314 0.001126 0.000426 0.000585 0.000391 0.000329 0.000575
Cytoplasm 0.089030 0.080200 0.011173 0.131890* 0.072902 0.080342 0.025599 0.206534* 0.083398 0.080295 0.012584 0.181659*
Cytoplasmic Foci 0.022180 0.040350 0.000971 0.012942 0.018414 0.019556 0.007928 0.017115 0.020865 0.026341 0.001652 0.015724
Eisosomes 0.000204 0.000232 0.000015 0.000193 0.000314 0.000143 0.000079 0.000129 0.000243 0.000172 0.000022 0.000150
Endoplasmic Reticulum 0.730896* 0.707869* 0.748075* 0.529264* 0.814037* 0.763777* 0.651567* 0.379758* 0.759929* 0.745534* 0.738636* 0.429580*
Endosome 0.006450 0.014495 0.008439 0.012466 0.004823 0.009123 0.011960 0.014307 0.005882 0.010876 0.008783 0.013693
Golgi 0.014416 0.019602 0.000273 0.002098 0.013900 0.012135 0.000783 0.002060 0.014236 0.014571 0.000323 0.002073
Lipid Particles 0.050025 0.040326 0.002519 0.016118 0.028773 0.040613 0.015730 0.020166 0.042604 0.040519 0.003811 0.018817
Mitochondria 0.014495 0.007908 0.000248 0.001533 0.006876 0.007542 0.000184 0.004862 0.011835 0.007661 0.000242 0.003753
Mitotic Spindle 0.000613 0.000721 0.000257 0.008138 0.000830 0.000312 0.000365 0.009958 0.000688 0.000445 0.000268 0.009352
None 0.011585 0.007769 0.000133 0.001496 0.005357 0.005143 0.000056 0.001920 0.009410 0.006000 0.000126 0.001779
Nuclear Periphery 0.014733 0.012008 0.117444* 0.044909 0.005773 0.017386 0.079819 0.062491 0.011604 0.015631 0.113765* 0.056632
Nuclear Periphery Foci 0.022156 0.031719 0.093570 0.095966 0.012143 0.018495 0.139583 0.052367 0.018659 0.022810 0.098070 0.066896
Nucleolus 0.000229 0.000373 0.000050 0.000300 0.000144 0.000202 0.000280 0.000707 0.000199 0.000258 0.000072 0.000572
Nucleus 0.009607 0.007299 0.002284 0.054703 0.005359 0.011306 0.009427 0.106078 0.008124 0.009999 0.002982 0.088958
Peroxisomes 0.003391 0.002567 0.000027 0.000408 0.001873 0.001810 0.000278 0.000761 0.002861 0.002057 0.000052 0.000643
Vacuole 0.004668 0.013089 0.010480 0.070378 0.004100 0.007974 0.051900 0.092625 0.004469 0.009643 0.014531 0.085211
Vacuole Periphery 0.002379 0.004892 0.000493 0.000854 0.001571 0.001847 0.000456 0.002464 0.002097 0.002841 0.000489 0.001927

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.15 -0.67 -2.40 0.18 -0.70 1.07 -0.06 -3.67 -4.64 -3.63 -1.53 -0.69 -4.42 -2.97 -0.78
Bud Neck -1.39 -2.11 -7.31 -4.31 -5.76 0.30 -1.12 -13.21 -13.39 -4.65 -1.19 -2.41 -15.09 -13.56 -11.70
Bud Site -3.43 0.09 -5.74 -2.78 -5.57 0.18 -0.45 -8.93 -9.02 -8.01 -2.63 -0.02 -10.63 -9.16 -10.06
Cell Periphery 0.62 4.91 -0.95 -1.11 -2.21 2.95 -0.72 1.78 -2.59 0.92 3.88 2.60 0.19 -1.82 -1.84
Cytoplasm 1.55 23.53 -7.95 -8.09 -21.78 -1.54 4.52 -23.89 -23.34 -18.23 0.86 25.40 -24.17 -23.78 -43.98
Cytoplasmic Foci -5.19 14.08 4.34 7.80 -12.09 -0.54 2.48 -0.45 0.19 -2.88 -2.99 15.71 2.40 5.38 -18.27
Eisosomes -0.42 7.75 0.18 0.46 -2.92 2.20 2.82 2.23 0.15 -1.43 1.83 7.04 2.19 0.45 -5.18
Endoplasmic Reticulum 1.82 -1.49 19.94 14.71 18.89 4.91 5.15 45.82* 43.19* 8.73 1.88 2.29 47.79 42.88* 32.73
Endosome -7.90 -3.03 -7.13 1.41 -4.10 -5.25 -3.43 -11.06 -4.45 -0.68 -7.72 -4.77 -12.27 -3.15 -5.57
Golgi -2.53 14.91 9.87 8.85 -1.95 1.03 9.93 8.74 8.60 -2.00 -0.27 18.11 14.20 12.21 -3.61
Lipid Particles 2.56 20.00 12.84 7.12 -10.37 -3.75 2.78 3.67 8.30 -0.97 0.84 21.20 12.88 10.84 -14.55
Mitochondria 4.00 13.02 11.10 4.93 -2.88 -0.49 5.78 1.85 3.30 -9.26 3.95 14.10 9.33 5.55 -9.44
Mitotic Spindle -0.30 1.76 -4.71 -4.60 -4.90 1.06 0.77 -6.69 -7.49 -7.33 1.05 1.80 -8.46 -8.77 -8.93
None 1.82 9.50 7.30 3.38 -2.01 0.14 4.40 2.74 3.88 -3.62 2.94 10.41 7.91 5.13 -4.00
Nuclear Periphery 1.29 -15.98 -8.99 -9.09 10.90 -6.70 -4.53 -18.73 -13.22 1.28 -2.82 -17.07 -18.92 -15.92 9.86
Nuclear Periphery Foci -3.33 -15.43 -16.48 -12.71 0.01 -3.53 -7.17 -15.72 -12.76 4.95 -2.68 -17.70 -20.89 -17.86 6.88
Nucleolus -1.54 6.67 -1.64 0.34 -4.36 -0.98 -1.08 -7.14 -6.72 -2.06 -1.36 3.33 -7.51 -5.65 -9.03
Nucleus 2.11 9.35 -15.05 -15.52 -17.30 -5.37 -1.64 -34.14 -31.53 -21.93 -2.25 6.99 -36.06 -34.63 -37.92
Peroxisomes 1.61 8.35 7.21 6.53 -6.20 0.18 5.89 3.91 4.70 -4.32 2.49 10.14 7.61 7.70 -10.56
Vacuole -5.29 -5.06 -18.17 -15.80 -16.56 -4.24 -3.95 -30.86 -29.42 -3.77 -6.49 -6.10 -35.66 -33.19 -27.32
Vacuole Periphery -3.23 5.82 4.50 5.61 -1.87 -0.79 3.45 -1.25 -0.76 -4.21 -2.11 6.71 1.08 2.99 -4.54
Standard name
Human Ortholog
Description Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Pmt7

Pmt7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pmt7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available