Standard name | |
---|---|
Human Ortholog | |
Description | Redundant rho-like GTPase Cdc42p effector; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region; GIC2 has a paralog, GIC1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 6 | 6 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 8 | 9 | 1 | 3 | 0 | 0 | 8 | 2 | 32 | 37 | 33 | 22 |
Bud | 1 | 1 | 1 | 3 | 5 | 5 | 32 | 11 | 18 | 15 | 23 | 13 | 5 | 3 | 0 | 1 | 0 | 0 | 5 | 4 | 25 | 46 | 32 | 29 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 3 | 18 | 48 | 26 | 22 |
Bud Site | 0 | 1 | 3 | 2 | 0 | 1 | 1 | 0 | 1 | 3 | 3 | 4 | 2 | 0 | 0 | 5 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 0 | 32 | 34 | 12 | 3 | 13 | 12 | 9 | 12 | 7 | 11 | 21 | 36 | 24 | 6 | 7 | 10 | 1 | 0 | 14 | 4 | 5 | 6 |
Cytoplasm | 127 | 80 | 357 | 313 | 404 | 468 | 593 | 631 | 388 | 412 | 341 | 320 | 317 | 525 | 486 | 163 | 112 | 109 | 107 | 68 | 262 | 95 | 173 | 287 |
Endoplasmic Reticulum | 1 | 0 | 3 | 2 | 1 | 2 | 2 | 5 | 4 | 2 | 1 | 1 | 3 | 6 | 3 | 8 | 8 | 4 | 0 | 0 | 12 | 0 | 4 | 2 |
Endosome | 0 | 0 | 6 | 4 | 5 | 3 | 1 | 10 | 1 | 1 | 4 | 5 | 2 | 1 | 1 | 3 | 0 | 0 | 0 | 2 | 4 | 4 | 5 | 4 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 1 | 1 | 0 |
Mitochondria | 8 | 4 | 6 | 37 | 61 | 143 | 331 | 282 | 251 | 228 | 302 | 202 | 0 | 0 | 0 | 5 | 4 | 2 | 2 | 1 | 20 | 2 | 2 | 1 |
Nucleus | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 2 | 2 | 2 | 3 | 4 | 3 | 1 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 1 | 6 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 5 | 2 | 4 | 1 | 3 | 7 | 3 | 3 | 2 | 4 | 2 | 8 | 2 | 8 | 2 | 0 | 1 | 0 | 3 | 1 | 1 | 0 |
Vac/Vac Membrane | 0 | 0 | 3 | 0 | 12 | 8 | 24 | 20 | 17 | 11 | 15 | 25 | 2 | 3 | 2 | 7 | 4 | 3 | 0 | 1 | 3 | 4 | 5 | 0 |
Unique Cell Count | 131 | 86 | 387 | 349 | 448 | 532 | 780 | 823 | 553 | 538 | 530 | 470 | 335 | 549 | 496 | 183 | 120 | 113 | 135 | 87 | 409 | 253 | 299 | 383 |
Labelled Cell Count | 139 | 88 | 425 | 409 | 507 | 635 | 1007 | 985 | 695 | 689 | 702 | 594 | 366 | 594 | 523 | 212 | 139 | 130 | 135 | 87 | 409 | 253 | 299 | 383 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.4 | 5.4 | 5.5 | 5.2 | 6.0 | 4.2 | 4.0 | 4.0 | 3.7 | 3.5 | 3.4 | 4.9 | 6.9 | 7.0 | 6.7 | 7.0 | 6.3 | 6.7 | 9.9 | 11.2 | 12.4 |
Std Deviation (1e-4) | 0.8 | 1.2 | 1.8 | 1.9 | 6.8 | 1.5 | 1.4 | 0.9 | 0.9 | 1.1 | 1.3 | 6.1 | 1.3 | 1.2 | 1.2 | 1.8 | 2.1 | 1.9 | 2.3 | 2.2 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.13 | -0.38 | -0.45 | -0.45 | -0.55 | -0.64 | -0.67 | -0.17 | 0.33 | 0.36 | 0.28 | 0.36 | 0.21 | 0.3 | 0.86 | 1.03 | 1.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.8 | 0.1 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.7 | -3.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.0 | -1.0 | -2.1 | -2.2 | -0.9 | 0.2 |
Cytoplasm | -1.2 | -1.0 | -2.1 | -6.7 | -6.5 | -8.2 | -6.3 | -9.8 | -8.6 | 1.3 | 2.2 | 4.1 | -1.3 | 0.4 | 1.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | -0.5 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 5.2 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.2 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 2.1 | 0 | 2.5 | 2.0 | 2.4 | 1.6 | 2.2 | 3.7 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.7078 | 3.4444 | 2.5517 | 2.1633 | 2.0388 | 2.8955 | 2.1952 | 3.4031 | 2.0621 | 1.4246 | 1.6014 | 2.2281 | 1.398 | 2.6923 | 0.9677 | 0.5684 | 0.5412 | 1.4099 |
Actin | 0.006 | 0.0048 | 0.0041 | 0.0322 | 0.0028 | 0.0073 | 0.0188 | 0.004 | 0.0165 | 0.0051 | 0.0298 | 0.0119 | 0.033 | 0.0027 | 0.0203 | 0.0007 | 0.0126 | 0.0028 |
Bud | 0.0035 | 0.0101 | 0.0066 | 0.0173 | 0.0005 | 0.0019 | 0.0011 | 0.001 | 0.0025 | 0.0007 | 0.0021 | 0.0006 | 0.0025 | 0.0035 | 0.0018 | 0.0025 | 0.0028 | 0.0008 |
Bud Neck | 0.0113 | 0.0009 | 0.0027 | 0.0021 | 0.03 | 0.0769 | 0.0028 | 0.0006 | 0.0014 | 0.0026 | 0.0074 | 0.0494 | 0.0149 | 0.0032 | 0.0052 | 0.0007 | 0.0024 | 0.0525 |
Bud Periphery | 0.0039 | 0.0022 | 0.0052 | 0.015 | 0.0006 | 0.0029 | 0.0013 | 0.0003 | 0.0023 | 0.0007 | 0.0025 | 0.0006 | 0.002 | 0.0027 | 0.0022 | 0.0019 | 0.0029 | 0.0009 |
Bud Site | 0.037 | 0.0279 | 0.0221 | 0.0328 | 0.0071 | 0.0039 | 0.0103 | 0.027 | 0.0078 | 0.0006 | 0.0067 | 0.0036 | 0.0308 | 0.083 | 0.0043 | 0.0004 | 0.0065 | 0.0003 |
Cell Periphery | 0.0027 | 0.0004 | 0.0008 | 0.002 | 0.0004 | 0.0006 | 0.0017 | 0.0007 | 0.0002 | 0.0001 | 0.0009 | 0.0004 | 0.0032 | 0.002 | 0.0003 | 0.0001 | 0.0011 | 0.0002 |
Cytoplasm | 0.6908 | 0.7514 | 0.6767 | 0.6086 | 0.6935 | 0.728 | 0.7431 | 0.8614 | 0.7924 | 0.8318 | 0.7604 | 0.8379 | 0.6855 | 0.8359 | 0.856 | 0.8979 | 0.7406 | 0.8345 |
Cytoplasmic Foci | 0.0121 | 0.0106 | 0.0098 | 0.0143 | 0.0157 | 0.0155 | 0.0261 | 0.003 | 0.006 | 0.0504 | 0.0085 | 0.0145 | 0.0471 | 0.0048 | 0.0051 | 0.0103 | 0.0307 | 0.0077 |
Eisosomes | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0028 | 0.0013 | 0.0033 | 0.0024 | 0.0028 | 0.0015 | 0.0052 | 0.0019 | 0.003 | 0.0049 | 0.0016 | 0.0009 | 0.0133 | 0.0024 | 0.0047 | 0.0026 | 0.0031 | 0.0033 |
Endosome | 0.0028 | 0.0042 | 0.0037 | 0.0043 | 0.005 | 0.0025 | 0.0152 | 0.0009 | 0.0128 | 0.0085 | 0.0303 | 0.0039 | 0.016 | 0.0011 | 0.0075 | 0.0021 | 0.0093 | 0.0063 |
Golgi | 0.0008 | 0.0005 | 0.0004 | 0.0005 | 0.001 | 0.0009 | 0.0063 | 0.0001 | 0.0032 | 0.0024 | 0.0067 | 0.0038 | 0.0078 | 0.0003 | 0.002 | 0.0002 | 0.009 | 0.0023 |
Lipid Particles | 0.0006 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0004 | 0.0073 | 0.0001 | 0.0033 | 0.0038 | 0.0077 | 0.0025 | 0.0137 | 0 | 0.001 | 0.0001 | 0.0203 | 0.0003 |
Mitochondria | 0.0006 | 0.0012 | 0.0009 | 0.0012 | 0.0006 | 0.0015 | 0.0098 | 0.0001 | 0.0093 | 0.0005 | 0.0177 | 0.0133 | 0.0015 | 0.0036 | 0.0046 | 0.0003 | 0.0403 | 0.0043 |
None | 0.217 | 0.1758 | 0.247 | 0.2515 | 0.2272 | 0.1503 | 0.1052 | 0.0963 | 0.1234 | 0.0558 | 0.088 | 0.0421 | 0.0998 | 0.0514 | 0.074 | 0.0757 | 0.0767 | 0.0783 |
Nuclear Periphery | 0.0008 | 0.0009 | 0.002 | 0.0018 | 0.001 | 0.0004 | 0.0152 | 0.0004 | 0.0013 | 0.0022 | 0.0028 | 0.0005 | 0.0046 | 0.0005 | 0.0052 | 0.0006 | 0.0009 | 0.0009 |
Nucleolus | 0.0006 | 0.0003 | 0.0005 | 0.0004 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Nucleus | 0.0019 | 0.0033 | 0.007 | 0.0071 | 0.0042 | 0.0019 | 0.009 | 0.0012 | 0.0043 | 0.0032 | 0.0029 | 0.0023 | 0.0014 | 0.0013 | 0.0024 | 0.0016 | 0.0013 | 0.0019 |
Peroxisomes | 0.0008 | 0.0012 | 0.0005 | 0.0009 | 0.0029 | 0.0018 | 0.0089 | 0 | 0.0055 | 0.0113 | 0.0161 | 0.0097 | 0.0183 | 0.0004 | 0.0005 | 0.0005 | 0.0361 | 0.0001 |
Punctate Nuclear | 0.0025 | 0.0009 | 0.0047 | 0.003 | 0.0031 | 0.0012 | 0.008 | 0.0002 | 0.0006 | 0.0129 | 0.0006 | 0.0009 | 0.0024 | 0.0003 | 0.0005 | 0.0008 | 0.0018 | 0.001 |
Vacuole | 0.0009 | 0.0012 | 0.0012 | 0.0019 | 0.0008 | 0.0005 | 0.002 | 0.0006 | 0.0031 | 0.0019 | 0.0045 | 0.0006 | 0.0012 | 0.0007 | 0.0015 | 0.0008 | 0.001 | 0.0009 |
Vacuole Periphery | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0015 | 0.0001 | 0.0007 | 0.0003 | 0.0026 | 0.0003 | 0.0005 | 0.0001 | 0.0008 | 0.0001 | 0.0003 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 31.129 | 28.2556 | 22.8729 | 54.4705 | 58.367 | 35.4196 | 20.7776 | 18.698 | 27.908 | 67.2684 |
Translational Efficiency | 1.8591 | 1.2607 | 1.2497 | 1.1932 | 1.8869 | 2.4353 | 1.5407 | 1.3115 | 1.9605 | 1.7775 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✔ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1574 | 565 | 317 | 1317 | 1761 | 916 | 240 | 129 | 3335 | 1481 | 557 | 1446 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 736.04 | 745.92 | 1067.94 | 978.64 | 727.18 | 794.95 | 1031.39 | 1195.88 | 731.36 | 776.25 | 1052.19 | 998.02 |
Standard Deviation | 96.45 | 118.13 | 138.71 | 189.25 | 106.34 | 113.89 | 145.02 | 168.11 | 101.89 | 117.95 | 142.62 | 197.43 |
Intensity Change Log 2 | — | 0.019237 | 0.536975 | 0.410994 | — | 0.128552 | 0.504206 | 0.717688 | — | 0.074598 | 0.520782 | 0.571550 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001041 | 0.002484 | 0.004359 | 0.005523 | 0.000629 | 0.000865 | 0.001635 | 0.002137 | 0.000823 | 0.001483 | 0.003185 | 0.005221 |
Bud Neck | 0.030834 | 0.035423 | 0.008531 | 0.012487 | 0.036113 | 0.031469 | 0.002740 | 0.010646 | 0.033622 | 0.032978 | 0.006036 | 0.012323 |
Bud Site | 0.031280 | 0.068133 | 0.058557 | 0.125223* | 0.042999 | 0.045500 | 0.029650 | 0.164068* | 0.037468 | 0.054134 | 0.046102 | 0.128688* |
Cell Periphery | 0.000490 | 0.000365 | 0.000633 | 0.000513 | 0.000428 | 0.000490 | 0.000519 | 0.000262 | 0.000458 | 0.000442 | 0.000584 | 0.000490 |
Cytoplasm | 0.543975* | 0.417788* | 0.356035* | 0.535684* | 0.571488* | 0.524098* | 0.418356* | 0.449022* | 0.558503* | 0.483541* | 0.382888* | 0.527953* |
Cytoplasmic Foci | 0.200735* | 0.227124* | 0.029663 | 0.034796 | 0.157406 | 0.185230* | 0.035873 | 0.053004 | 0.177856 | 0.201213* | 0.032339 | 0.036420 |
Eisosomes | 0.000321 | 0.000238 | 0.000161 | 0.000130 | 0.000299 | 0.000278 | 0.000133 | 0.000066 | 0.000310 | 0.000262 | 0.000149 | 0.000124 |
Endoplasmic Reticulum | 0.001373 | 0.001703 | 0.010116 | 0.000812 | 0.001453 | 0.001621 | 0.006419 | 0.001057 | 0.001415 | 0.001652 | 0.008523 | 0.000834 |
Endosome | 0.008864 | 0.025160 | 0.009158 | 0.002585 | 0.008834 | 0.013916 | 0.006521 | 0.004242 | 0.008848 | 0.018205 | 0.008021 | 0.002732 |
Golgi | 0.003159 | 0.004856 | 0.000267 | 0.001587 | 0.002192 | 0.002687 | 0.000202 | 0.000253 | 0.002648 | 0.003514 | 0.000239 | 0.001468 |
Lipid Particles | 0.004302 | 0.005336 | 0.004939 | 0.001116 | 0.004372 | 0.003620 | 0.003364 | 0.001417 | 0.004339 | 0.004275 | 0.004260 | 0.001143 |
Mitochondria | 0.005067 | 0.004581 | 0.000356 | 0.000225 | 0.002904 | 0.002315 | 0.000227 | 0.000059 | 0.003925 | 0.003180 | 0.000300 | 0.000210 |
Mitotic Spindle | 0.001172 | 0.004115 | 0.001641 | 0.003387 | 0.000785 | 0.001852 | 0.001163 | 0.000763 | 0.000967 | 0.002716 | 0.001435 | 0.003153 |
None | 0.001431 | 0.004155 | 0.001925 | 0.004986 | 0.003974 | 0.001011 | 0.001643 | 0.000713 | 0.002774 | 0.002211 | 0.001804 | 0.004605 |
Nuclear Periphery | 0.000242 | 0.000226 | 0.001598 | 0.000170 | 0.000219 | 0.000221 | 0.001233 | 0.000348 | 0.000230 | 0.000223 | 0.001440 | 0.000186 |
Nuclear Periphery Foci | 0.000477 | 0.000881 | 0.009107 | 0.001562 | 0.000476 | 0.000538 | 0.005610 | 0.004664 | 0.000476 | 0.000669 | 0.007600 | 0.001839 |
Nucleolus | 0.000363 | 0.001500 | 0.000272 | 0.000819 | 0.000527 | 0.000372 | 0.000240 | 0.000065 | 0.000450 | 0.000802 | 0.000258 | 0.000752 |
Nucleus | 0.098627 | 0.056076 | 0.228068* | 0.107599 | 0.100332 | 0.080691 | 0.228403* | 0.036065 | 0.099527 | 0.071300 | 0.228212* | 0.101217 |
Peroxisomes | 0.004961 | 0.016051 | 0.001532 | 0.001815 | 0.002842 | 0.005228 | 0.000631 | 0.003342 | 0.003842 | 0.009357 | 0.001144 | 0.001951 |
Vacuole | 0.060447 | 0.122376 | 0.272775* | 0.158853 | 0.060905 | 0.097155 | 0.255200* | 0.267683* | 0.060689 | 0.106777 | 0.265202* | 0.168561 |
Vacuole Periphery | 0.000841 | 0.001429 | 0.000307 | 0.000129 | 0.000822 | 0.000842 | 0.000239 | 0.000121 | 0.000831 | 0.001066 | 0.000277 | 0.000128 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.62 | -2.66 | -4.81 | -2.19 | -1.30 | -1.39 | -2.27 | -1.74 | -0.96 | 0.50 | -2.72 | -3.39 | -5.39 | -4.08 | -2.31 |
Bud Neck | -0.92 | 8.27 | 8.11 | 7.30 | -0.21 | 1.28 | 12.14 | 10.75 | 7.85 | -1.63 | 0.43 | 14.09 | 12.09 | 9.57 | -1.73 |
Bud Site | -4.82 | -0.57 | -8.29 | -1.86 | -5.53 | -0.29 | 4.20 | -1.01 | -0.86 | -2.83 | -3.59 | 1.96 | -7.72 | -4.32 | -7.83 |
Cell Periphery | 0.97 | -0.36 | 0.68 | -0.71 | 2.74 | -0.65 | 0.07 | 7.03 | 3.55 | 3.48 | 0.27 | -0.44 | 1.10 | 0.84 | 2.32 |
Cytoplasm | 9.69 | 17.68 | 1.87 | -7.90 | -15.83 | 4.66 | 13.28 | 8.02 | 5.73 | -1.28 | 9.18 | 22.23 | 5.36 | -3.00 | -16.03 |
Cytoplasmic Foci | -2.64 | 34.20 | 33.36 | 22.57 | -4.29 | -3.81 | 27.92 | 18.80 | 18.77 | -2.93 | -3.89 | 44.20 | 42.66 | 32.74 | -4.50 |
Eisosomes | 1.94 | 4.80 | 6.42 | 4.38 | 4.19 | 0.92 | 7.90 | 13.36 | 9.52 | 7.01 | 1.99 | 8.30 | 10.69 | 8.30 | 4.70 |
Endoplasmic Reticulum | -0.64 | -12.61 | 1.95 | 1.65 | 13.62 | -0.23 | -8.06 | 0.83 | 0.62 | 8.54 | -0.50 | -14.77 | 2.64 | 1.58 | 16.13 |
Endosome | -6.80 | 0.62 | 9.28 | 10.23 | 9.84 | -3.88 | 3.35 | 7.18 | 9.24 | 4.62 | -7.64 | 2.38 | 13.06 | 14.49 | 11.84 |
Golgi | -1.78 | 5.02 | 2.94 | 4.59 | -1.24 | -0.71 | 8.29 | 8.39 | 4.66 | 0.97 | -1.50 | 8.05 | 3.31 | 4.19 | -1.28 |
Lipid Particles | -1.28 | -1.07 | 9.39 | 5.38 | 9.51 | 1.61 | 1.69 | 6.32 | 4.09 | 4.67 | 0.23 | 0.17 | 12.20 | 7.77 | 11.42 |
Mitochondria | 0.30 | 3.95 | 4.14 | 2.65 | 4.84 | 0.60 | 3.92 | 4.17 | 2.53 | 5.66 | 0.79 | 5.42 | 5.67 | 3.60 | 4.78 |
Mitotic Spindle | -1.64 | -1.50 | -2.33 | -0.12 | -1.09 | -1.25 | -0.72 | -1.14 | 0.70 | -0.56 | -2.00 | -1.77 | -2.67 | -0.94 | -1.68 |
None | -1.36 | -0.85 | -1.90 | 0.39 | -1.34 | 3.72 | 3.25 | 4.03 | 0.43 | 2.31 | 0.74 | 2.23 | -0.22 | -0.75 | -1.51 |
Nuclear Periphery | 0.19 | -16.66 | -0.07 | -0.38 | 18.03 | -0.23 | -12.74 | -3.71 | -3.85 | 7.93 | -0.02 | -20.83 | -1.29 | -1.72 | 21.67 |
Nuclear Periphery Foci | -2.95 | -10.68 | -10.53 | -5.51 | 8.49 | -0.67 | -11.44 | -6.04 | -5.91 | 1.34 | -2.52 | -14.55 | -12.18 | -9.68 | 10.33 |
Nucleolus | -2.25 | 1.24 | -0.38 | 1.76 | -0.58 | 1.56 | 2.96 | 4.44 | 8.57 | 7.83 | -1.69 | 3.06 | 0.08 | 1.11 | -0.64 |
Nucleus | 8.63 | -11.90 | 1.27 | -6.63 | 12.19 | 4.00 | -10.13 | 6.65 | 4.18 | 12.53 | 8.02 | -15.60 | 2.69 | -3.62 | 16.05 |
Peroxisomes | -4.65 | 6.51 | 6.06 | 6.43 | -1.02 | -3.18 | 6.46 | 2.53 | 4.77 | -1.86 | -5.33 | 8.67 | 6.68 | 8.15 | -2.11 |
Vacuole | -9.54 | -20.17 | -24.11 | -11.31 | 7.17 | -8.59 | -16.15 | -14.84 | -13.15 | -3.57 | -12.75 | -25.82 | -27.54 | -18.40 | 6.92 |
Vacuole Periphery | -2.62 | 3.57 | 4.82 | 8.70 | 6.03 | -0.04 | 4.82 | 5.79 | 6.34 | 4.38 | -1.61 | 5.80 | 7.42 | 10.28 | 6.83 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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