Standard name
Human Ortholog
Description Transcriptional repressor that regulates middle-sporulation genes; required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.14 0.15 0.11 0.06 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.25 0.55 0.4 0.22 0.47 0.5 0.5 0.63 0.67 0.64 0.7 0 0 0.1 0.08 0 0.1 0 0 0 0 0 0
Nucleus 0.87 0.88 0.88 0.86 0.85 0.75 0.76 0.73 0.65 0.62 0.67 0.6 0.88 0.79 0.75 0.77 0.82 0.79 0.74 0.81 0.77 0.78 0.74 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.22 0.17 0.19 0.11 0.13 0.2 0.19 0.16 0.25 0.25 0.3 0.3 0.22 0.15 0.18 0.12 0.11 0.15 0.19 0.08 0.15 0.17 0.16 0.26
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2 0 1 2 1 9 2 4 2 0 1 0 0 0 1 6 0 0 4 2
Bud Neck 0 0 0 0 0 1 0 0 0 0 1 2 0 0 0 0 0 0 0 0 0 0 1 1
Bud Site 0 1 3 4 3 10 13 13 8 14 10 10 1 0 2 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 4 0 3 9 10 7 7 3 6 5 3 12 66 75 21 8 9 0 0 0 0 0 1
Endoplasmic Reticulum 1 0 0 1 0 0 0 1 0 0 0 0 0 1 1 2 3 1 0 0 0 0 0 0
Endosome 0 1 0 0 0 0 1 0 0 0 0 1 0 2 0 1 2 2 2 1 0 1 2 9
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 1 0 1 1 3 2 1 0 0 0 1
Mitochondria 2 71 90 85 91 192 275 280 243 322 263 308 11 6 49 16 1 18 2 2 1 0 2 4
Nucleus 175 247 145 182 347 304 419 409 251 296 277 263 228 381 381 146 118 137 166 236 125 187 327 249
Nuclear Periphery 4 3 1 0 13 4 15 9 12 12 17 10 0 7 9 4 3 4 2 0 2 0 0 2
Nucleolus 44 48 31 24 54 79 103 90 96 120 122 129 58 73 93 22 16 26 42 22 23 40 72 110
Peroxisomes 0 0 0 0 1 1 1 0 0 1 0 0 1 2 2 0 0 0 0 0 0 0 0 0
SpindlePole 2 1 2 0 2 2 3 4 2 6 1 0 1 4 2 0 2 1 1 3 1 0 3 7
Vac/Vac Membrane 1 4 0 1 2 0 2 1 2 0 0 1 1 0 3 4 2 3 3 12 6 7 26 32
Unique Cell Count 202 280 164 211 408 405 549 563 388 481 411 437 259 481 509 189 144 173 227 291 162 241 444 432
Labelled Cell Count 230 380 273 301 524 603 840 816 618 786 698 731 315 543 618 217 156 204 227 291 162 241 444 432


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 5.3 4.2 4.6 4.6 4.1 3.8 4.0 3.6 3.5 3.6 3.3 6.6 6.6 5.6 7.7 8.6 8.8 6.5 6.3 7.1
Std Deviation (1e-4) 1.5 1.2 0.5 1.1 1.0 0.8 0.7 0.7 0.7 0.7 0.8 0.6 1.5 1.7 1.5 1.8 2.0 2.0 1.5 1.4 1.6
Intensity Change (Log2) – – – 0.14 0.13 -0.03 -0.13 -0.08 -0.24 -0.26 -0.22 -0.36 0.66 0.66 0.43 0.88 1.03 1.07 0.64 0.58 0.77


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 5.0 5.2 4.4 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 -11.9 -16.7 -12.4 -9.5 -10.4 -8.7
Nucleus -0.6 -1.0 -3.5 -3.3 -4.2 -5.7 -6.4 -5.1 -6.6 -0.1 -2.6 -3.7 -2.7 -1.6 -2.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.9 -1.1 -0.2 -1.9 -1.9 -0.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.9642 4.4511 3.913 3.4708 3.5615 3.9238 5.2787 5.5768 5.0223 5.1386 4.8716 5.0301 3.9422 3.8653 3.5177 3.2275 3.1316 3.458
Actin 0.0677 0 0.0066 0 0.0179 0.0026 0.0559 0 0.026 0.0003 0.029 0.0072 0 0 0.0043 0.0382 0.0001 0.002
Bud 0.0009 0 0.0003 0 0.0002 0.0001 0.0003 0 0.0004 0.0001 0.0004 0.0004 0 0 0.0006 0.0004 0.0001 0.0002
Bud Neck 0.0043 0.0001 0.0005 0 0.0015 0.0013 0.0013 0 0.0032 0.0003 0.0005 0.0017 0.0001 0.0001 0.0005 0.0006 0.0007 0.001
Bud Periphery 0.0018 0 0.0006 0 0.0004 0.0001 0.0006 0 0.0011 0.0001 0.001 0.001 0 0 0.0011 0.0007 0.0003 0.0005
Bud Site 0.014 0 0.0038 0 0.002 0.0001 0.0022 0 0.0021 0.0003 0.0013 0.0018 0 0 0.008 0.0012 0.0002 0.0004
Cell Periphery 0.0007 0 0.0003 0 0.0001 0 0.0003 0 0.0003 0 0.0002 0.0002 0 0 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.0041 0 0.0014 0 0.0009 0.0006 0.0162 0 0.0041 0.0021 0.0009 0.0004 0 0 0.0014 0.0006 0 0.0001
Cytoplasmic Foci 0.0337 0 0.0041 0 0.0141 0.0008 0.0124 0 0.008 0.0083 0.0132 0.0016 0 0 0.0077 0.0088 0 0.0019
Eisosomes 0.0006 0 0.0001 0 0.0004 0 0.0012 0 0.0002 0 0.0002 0 0 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0031 0 0.0013 0 0.0019 0.0006 0.0056 0 0.0048 0.0007 0.0025 0.002 0 0 0.0003 0.0006 0 0.0003
Endosome 0.0151 0 0.0056 0 0.009 0.0034 0.0131 0 0.0111 0.0161 0.0202 0.0089 0 0 0.0053 0.0117 0 0.0041
Golgi 0.0103 0 0.0011 0 0.0084 0.0017 0.0064 0 0.0079 0.0011 0.02 0.0043 0 0 0.003 0.014 0 0.0026
Lipid Particles 0.0389 0 0.0042 0 0.017 0.0033 0.0169 0 0.0066 0.0047 0.0396 0.0029 0 0 0.0039 0.0058 0 0.0067
Mitochondria 0.0078 0.0001 0.0116 0.0001 0.0061 0.0007 0.0057 0.0004 0.0167 0.0009 0.0492 0.0037 0.0003 0.0001 0.0048 0.0179 0.0029 0.0127
None 0.0097 0 0.0016 0 0.0024 0.0004 0.0068 0 0.0015 0.0009 0.0005 0.0002 0 0 0.0002 0.0006 0.0001 0.0001
Nuclear Periphery 0.0116 0.0008 0.0132 0.0002 0.0336 0.0064 0.0283 0.0018 0.0047 0.0126 0.0051 0.0047 0.0002 0.0005 0.0039 0.0051 0.0019 0.003
Nucleolus 0.0815 0.0535 0.0417 0.0381 0.2377 0.1493 0.0496 0.0355 0.04 0.0477 0.1392 0.0888 0.043 0.0515 0.0522 0.037 0.3169 0.1347
Nucleus 0.6242 0.9439 0.8882 0.9614 0.5998 0.8238 0.7569 0.9614 0.8497 0.8645 0.6656 0.8657 0.956 0.9468 0.89 0.8426 0.5878 0.8219
Peroxisomes 0.0307 0 0.0019 0 0.0112 0.0009 0.0078 0 0.0037 0.001 0.0027 0.0006 0 0 0.0075 0.0101 0 0.0012
Punctate Nuclear 0.0318 0.0013 0.0081 0.0002 0.0326 0.0029 0.0096 0.0005 0.0049 0.0374 0.0053 0.0012 0.0003 0.0009 0.0036 0.002 0.0882 0.005
Vacuole 0.0056 0.0001 0.0021 0 0.0009 0.0004 0.0013 0.0001 0.0015 0.0005 0.0012 0.0018 0 0 0.001 0.001 0.0002 0.0007
Vacuole Periphery 0.0019 0 0.0017 0 0.0019 0.0005 0.0015 0.0001 0.0016 0.0004 0.002 0.001 0 0 0.0003 0.0006 0.0002 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 21.6252 13.6095 16.7548 20.0345 26.9165 19.7113 18.0607 18.0471 20.7691 29.2853
Translational Efficiency 0.9768 1.5167 1.0154 1.218 0.6438 1.1208 1.3144 1.0118 0.8442 0.6828

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2244 1040 122 1119 2049 1740 1377 42 4293 2780 1499 1161

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 699.16 869.19 1161.45 942.18 819.80 830.36 962.35 974.88 756.74 844.89 978.55 943.36
Standard Deviation 85.99 141.05 164.53 136.65 118.02 158.62 144.18 158.39 118.93 153.44 155.77 137.64
Intensity Change Log 2 — 0.314049 0.732232 0.430380 — 0.018465 0.231290 0.249953 — 0.162069 0.483564 0.335812

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000149 0.000726 0.002488 0.001947 0.000298 0.000429 0.001049 0.001234 0.000220 0.000540 0.001166 0.001921
Bud Neck 0.002356 0.005943 0.013079 0.038576 0.006607 0.007256 0.014602 0.023484 0.004385 0.006765 0.014478 0.038030
Bud Site 0.000386 0.000510 0.002583 0.014269 0.000417 0.000826 0.001477 0.003312 0.000401 0.000708 0.001567 0.013873
Cell Periphery 0.000054 0.000040 0.000087 0.000183 0.000026 0.000038 0.000149 0.000192 0.000041 0.000038 0.000144 0.000184
Cytoplasm 0.003133 0.000482 0.000771 0.005674 0.000903 0.000878 0.005833 0.031050 0.002069 0.000730 0.005421 0.006592
Cytoplasmic Foci 0.000913 0.000233 0.000137 0.003073 0.000169 0.001132 0.000688 0.000645 0.000558 0.000796 0.000643 0.002985
Eisosomes 0.000016 0.000031 0.000067 0.000046 0.000022 0.000027 0.000046 0.000057 0.000019 0.000028 0.000048 0.000046
Endoplasmic Reticulum 0.000733 0.002180 0.006288 0.007149 0.001406 0.002148 0.003258 0.003819 0.001054 0.002160 0.003505 0.007028
Endosome 0.000226 0.000319 0.000299 0.007906 0.000160 0.001538 0.000310 0.000575 0.000195 0.001082 0.000309 0.007641
Golgi 0.000191 0.000313 0.000352 0.002763 0.000129 0.000600 0.000206 0.000211 0.000161 0.000493 0.000218 0.002671
Lipid Particles 0.000560 0.000228 0.000273 0.000801 0.000103 0.000728 0.000178 0.000588 0.000342 0.000541 0.000186 0.000793
Mitochondria 0.001349 0.002757 0.001760 0.006704 0.000634 0.003407 0.001470 0.001875 0.001008 0.003164 0.001494 0.006529
Mitotic Spindle 0.001343 0.003068 0.001279 0.058504 0.001807 0.004535 0.007499 0.113653* 0.001564 0.003986 0.006992 0.060499
None 0.009793 0.003124 0.001524 0.004432 0.002494 0.002235 0.014043 0.009387 0.006309 0.002568 0.013024 0.004611
Nuclear Periphery 0.000335 0.000190 0.000353 0.002461 0.000170 0.000739 0.000403 0.000602 0.000257 0.000533 0.000399 0.002393
Nuclear Periphery Foci 0.000492 0.000339 0.000585 0.001654 0.000372 0.000846 0.000352 0.000518 0.000435 0.000657 0.000371 0.001613
Nucleolus 0.147867 0.131605 0.086059 0.051917 0.120525 0.122208 0.083033 0.052173 0.134817 0.125724 0.083279 0.051926
Nucleus 0.829346* 0.846411* 0.876690* 0.779236* 0.862991* 0.847191* 0.863424* 0.752517* 0.845404* 0.846899* 0.864503* 0.778270*
Peroxisomes 0.000340 0.001108 0.002663 0.001491 0.000355 0.000843 0.000866 0.000769 0.000347 0.000942 0.001012 0.001465
Vacuole 0.000310 0.000161 0.002362 0.006767 0.000251 0.001203 0.000873 0.002994 0.000282 0.000813 0.000994 0.006630
Vacuole Periphery 0.000108 0.000232 0.000301 0.004446 0.000160 0.001193 0.000242 0.000343 0.000133 0.000834 0.000246 0.004297

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.88 -11.18 -16.48 -10.98 1.15 -3.81 -22.48 -5.09 -4.79 -2.69 -8.47 -26.88 -16.52 -13.86 -7.17
Bud Neck -7.70 -6.04 -22.09 -19.99 -9.45 -1.21 -14.42 -2.56 -2.45 -0.69 -6.35 -18.60 -21.25 -19.88 -13.20
Bud Site -0.95 -6.64 -6.55 -6.48 -4.08 -1.55 -10.02 -5.56 -5.04 -3.69 -1.84 -12.73 -6.66 -6.43 -5.34
Cell Periphery 1.47 -2.95 -5.04 -6.20 -2.42 -2.17 -4.17 -3.53 -3.22 -0.90 0.42 -3.71 -6.48 -6.77 -0.95
Cytoplasm 4.12 3.40 -1.78 -8.10 -6.99 0.03 -3.76 -2.03 -2.03 -1.78 3.44 -2.19 -4.36 -6.89 -1.61
Cytoplasmic Foci 2.10 2.37 -0.13 -2.34 -2.63 -2.87 -1.47 -1.51 1.49 -0.21 -0.67 0.57 -1.13 -0.62 -1.55
Eisosomes -8.79 -8.62 -17.64 -9.98 2.43 -4.19 -14.84 -5.12 -4.64 -2.50 -9.35 -18.25 -17.78 -12.86 -1.38
Endoplasmic Reticulum -11.35 -6.53 -13.40 -10.32 -0.74 -5.14 -14.30 -3.62 -3.17 -2.16 -11.05 -18.03 -13.16 -10.79 -6.74
Endosome -0.38 -1.51 -3.76 -3.59 -3.57 -3.16 -2.53 -3.42 1.49 -2.55 -3.11 -2.45 -3.84 -2.54 -3.66
Golgi -0.81 -3.21 -3.43 -2.98 -2.67 -2.80 -5.99 -3.82 1.61 -1.14 -2.77 -3.55 -3.60 -2.33 -3.23
Lipid Particles 1.28 0.84 0.20 -1.89 -1.24 -2.61 -2.02 -2.80 0.24 -2.42 -0.90 1.11 -0.95 0.07 -2.72
Mitochondria -1.37 -1.54 -7.38 -2.66 -7.57 -3.33 -12.19 -5.27 1.17 -1.73 -3.28 -4.30 -9.10 -2.97 -7.91
Mitotic Spindle -1.38 -0.56 -11.89 -11.30 -11.83 -2.01 -4.46 -2.21 -2.15 -2.01 -2.58 -4.80 -12.03 -11.51 -10.27
None 6.02 7.64 4.48 -3.62 -8.78 0.58 -4.89 -1.90 -2.04 1.64 6.12 -2.16 1.97 -6.52 3.12
Nuclear Periphery 1.60 -1.11 -12.44 -12.94 -12.10 -3.26 -8.73 -3.85 -1.27 -2.31 -2.41 -5.91 -13.10 -10.96 -11.96
Nuclear Periphery Foci 1.74 -0.07 -2.98 -4.62 -2.79 -1.78 -0.21 -2.03 0.09 -2.01 -0.34 1.06 -3.84 -3.57 -4.46
Nucleolus 3.53 3.43 15.47 10.70 2.93 -0.17 4.25 2.75 2.78 1.61 3.24 8.09 15.50 12.55 5.75
Nucleus -3.46 -1.46 7.96 10.11 5.97 2.75 3.43 2.90 2.55 2.41 -1.11 0.11 10.91 11.26 9.84
Peroxisomes -3.44 -6.59 -4.42 -0.02 4.26 -3.72 -7.71 -2.65 -0.19 0.00 -4.99 -9.32 -4.86 -0.67 -0.02
Vacuole 1.02 -3.53 -7.00 -7.41 -2.54 -3.00 -5.02 -2.81 -2.57 -2.58 -2.13 -5.29 -7.52 -6.97 -6.39
Vacuole Periphery -1.93 -7.93 -2.55 -2.36 -2.20 -2.40 -2.40 -2.55 1.97 -1.02 -2.59 -3.78 -2.53 -1.31 -2.31
Standard name
Human Ortholog
Description Transcriptional repressor that regulates middle-sporulation genes; required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
Localization
Cell Percentages nucleus (98%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Sum1

Sum1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sum1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available