Standard name
Human Ortholog
Description Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.07 0 0.07 – 0 0.06 0 0 0.05 0.16 0.21 0.09 0.12 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.09 0 0 0 0 0.05 0 – 0.1 0.06 0.23 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.9 0.92 0.94 0.9 0.88 0.89 0.84 – 0.83 0.8 0.79 0.88 0.9 0.78 0.73 0.8 0.76 0.75 0.85 0.88 0.91 0.76 0.8 0.76
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.13 0 0.05 0 0.06 0.1 – 0.17 0.14 0.28 0.19 0.09 0 0 0.06 0.05 0 0.05 0.05 0 0.11 0.07 0.08
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.1 0.1 0 0 0 0 0.06 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 0 0 1 0 1 1 0 1 0
Bud Neck 0 0 0 0 0 0 1 – 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 4 – 7 2 7 3 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 6 4 7 17 17 13 24 – 10 21 6 9 5 30 40 16 11 13 0 0 0 0 1 2
Endoplasmic Reticulum 0 1 0 0 0 1 0 – 0 0 0 0 0 0 0 1 0 3 0 0 0 0 0 0
Endosome 0 0 1 1 2 0 0 – 2 2 0 1 1 1 1 1 0 1 5 2 0 2 5 1
Golgi 0 1 0 1 0 0 0 – 0 1 0 0 0 0 0 2 0 1 1 3 0 0 0 0
Mitochondria 25 5 1 1 2 16 13 – 32 21 64 21 2 1 1 4 1 4 4 2 1 0 1 2
Nucleus 248 192 199 219 224 260 308 – 269 284 220 286 87 143 138 135 67 101 232 201 204 73 165 161
Nuclear Periphery 1 0 0 1 1 1 1 – 1 4 2 3 1 0 1 1 0 0 0 0 1 0 0 0
Nucleolus 30 27 4 11 8 17 36 – 55 51 77 61 9 7 6 10 4 5 12 10 4 10 13 16
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 1 2 1 2 – 2 0 0 1 1 2 3 0 2 1 1 0 1 1 2 1
Vac/Vac Membrane 2 4 4 2 7 4 5 – 2 6 1 1 0 4 8 7 9 14 8 5 5 2 13 24
Unique Cell Count 276 209 212 244 254 293 368 324 354 278 326 97 183 190 169 88 134 273 229 225 97 206 213
Labelled Cell Count 313 234 218 254 263 314 394 380 392 377 386 107 188 199 178 94 144 273 229 225 97 206 213


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.4 8.5 8.7 8.2 8.4 7.1 7.2 – 6.3 6.7 5.8 6.3 8.1 9.5 9.6 10.6 13.3 12.0 6.9 8.1 8.7
Std Deviation (1e-4) 1.3 1.6 1.6 1.6 1.7 1.9 1.9 – 1.4 1.6 1.6 1.4 2.1 3.7 4.4 2.7 4.7 3.0 1.3 2.4 2.4
Intensity Change (Log2) – – – -0.08 -0.04 -0.28 -0.27 – -0.45 -0.36 -0.57 -0.45 -0.1 0.14 0.14 0.28 0.61 0.47 -0.32 -0.09 0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.7 1.6 0.6 1.7 – -0.1 1.4 -0.8 -0.4 0 4.5 5.5 2.5 3.1 2.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.1 2.3 – 4.4 3.3 7.3 3.4 0 0 0 0 0 0
Nucleus -1.6 -2.1 -2.0 -3.6 – -3.7 -4.4 -4.6 -2.3 -1.3 -4.6 -5.8 -4.1 -4.4 -4.9
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.6 0.9 2.2 3.6 – 5.5 4.9 7.6 5.9 0 1.2 0 2.1 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0.6 0 0 – 0 0 0 0 0 0 1.4 0 0 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0881 2.605 2.46 1.9282 1.6449 2.1382 3.7411 3.8318 3.7822 3.3987 3.6193 3.6026 3.3383 3.7829 3.0924 3.3314 2.9974 3.0425
Actin 0.0023 0.0001 0.0104 0.0096 0.0056 0 0.0013 0 0.0041 0.0014 0.0054 0.001 0.0315 0 0.0378 0 0.0153 0
Bud 0.0005 0 0.0002 0.0003 0.0002 0 0.0005 0 0.0002 0.0001 0.0003 0.0001 0.0002 0 0.0001 0 0.0008 0
Bud Neck 0.0004 0.0001 0.0001 0.0001 0.0006 0.0011 0.0025 0.0001 0.0019 0.0002 0.0004 0.0005 0.0019 0 0.0008 0 0.0031 0.0002
Bud Periphery 0.0009 0.0001 0.0001 0.0005 0.0005 0.0001 0.0015 0 0.0004 0.0001 0.0012 0.0003 0.0003 0 0.0001 0 0.0015 0
Bud Site 0.0022 0.0001 0.0007 0.0015 0.0006 0.0001 0.0029 0.0001 0.0027 0.0005 0.0012 0.0001 0.0006 0 0.0001 0 0.0041 0
Cell Periphery 0.0003 0 0 0.0001 0.0002 0 0.0006 0 0.0003 0.0002 0.0003 0.0001 0.0003 0 0 0 0.0004 0
Cytoplasm 0.0031 0 0.0124 0.0013 0.0013 0 0.0041 0 0.0008 0.0144 0.0002 0.0003 0.0024 0 0.0002 0 0.0038 0
Cytoplasmic Foci 0.0226 0.0035 0.002 0.0049 0.0146 0 0.0074 0 0.0046 0.0028 0.0097 0.0024 0.0006 0 0.0005 0 0.0047 0
Eisosomes 0 0 0.0001 0.0002 0.0001 0 0.0001 0 0.0003 0 0.0001 0 0.0004 0 0.0002 0 0.0003 0
Endoplasmic Reticulum 0.0015 0 0.0005 0.0009 0.0005 0 0.0028 0 0.0014 0.0146 0.0007 0.0003 0.0045 0 0.001 0 0.0044 0
Endosome 0.0313 0.0009 0.0064 0.0109 0.0106 0 0.0063 0 0.006 0.0221 0.0124 0.0099 0.0073 0 0.0028 0 0.0221 0
Golgi 0.0095 0.0008 0.0025 0.0032 0.0067 0 0.0011 0 0.0014 0.0083 0.0052 0.0037 0.0016 0 0.0017 0 0.0029 0
Lipid Particles 0.0237 0.0047 0.0008 0.0096 0.0275 0 0.0064 0 0.0072 0.0035 0.0461 0.0044 0.0006 0 0.0007 0 0.0033 0
Mitochondria 0.0053 0.0004 0.0045 0.0315 0.0469 0.0001 0.0038 0.0002 0.0073 0.0025 0.0087 0.0032 0.0013 0.0001 0.0006 0 0.03 0
None 0.0016 0 0.0003 0.0022 0.0005 0 0.0021 0 0.0009 0.0007 0.0004 0.0004 0.0022 0 0.001 0 0.0045 0
Nuclear Periphery 0.0141 0.0017 0.002 0.0071 0.0092 0.0003 0.0101 0.0004 0.0126 0.0199 0.0129 0.0027 0.018 0.0002 0.0084 0 0.0222 0
Nucleolus 0.0243 0.0156 0.0053 0.0037 0.1038 0.0626 0.0249 0.0172 0.0118 0.0088 0.093 0.0375 0.0192 0.0081 0.0066 0.0068 0.0975 0.024
Nucleus 0.8045 0.9684 0.9498 0.8981 0.7099 0.9352 0.9019 0.9806 0.9255 0.8944 0.7833 0.9277 0.8982 0.9915 0.9354 0.9931 0.7626 0.9755
Peroxisomes 0.0079 0.0031 0.0008 0.0075 0.0226 0 0.0072 0 0.0021 0.0003 0.0078 0.0013 0.0001 0 0.0004 0 0.0011 0
Punctate Nuclear 0.0362 0.0004 0.0005 0.0049 0.0308 0.0003 0.0055 0.0012 0.0055 0.0008 0.0032 0.0027 0.0069 0.0001 0.0012 0 0.0093 0.0001
Vacuole 0.0047 0.0001 0.0005 0.0007 0.0034 0.0001 0.0045 0.0001 0.0019 0.0022 0.0033 0.0007 0.0007 0 0.0001 0 0.0033 0
Vacuole Periphery 0.003 0.0001 0.0003 0.0012 0.0037 0 0.0025 0 0.0013 0.0021 0.0044 0.0006 0.0011 0 0.0003 0 0.0028 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.3002 14.3175 17.4541 17.8066 11.8661 15.4357 14.6057 16.7496 16.6667 20.0103
Translational Efficiency 1.4231 1.1025 1.1507 1.0833 2.0109 1.2803 1.1324 1.1241 1.2086 1.1657

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1647 1202 2632 1788 1321 1610 480 71 2968 2812 3112 1859

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 662.12 799.89 1151.85 1113.92 900.90 895.47 1182.24 1208.70 768.40 854.61 1156.54 1117.54
Standard Deviation 94.56 155.56 165.63 182.98 130.58 140.06 159.20 241.63 163.19 154.31 165.02 186.45
Intensity Change Log 2 — 0.272709 0.798788 0.750481 — -0.008722 0.392084 0.424017 — 0.117255 0.578524 0.571417

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000267 0.000775 0.000360 0.000669 0.000224 0.000457 0.000954 0.000990 0.000248 0.000593 0.000452 0.000681
Bud Neck 0.003311 0.006221 0.020053 0.024726 0.008573 0.009622 0.012576 0.015133 0.005653 0.008168 0.018899 0.024360
Bud Site 0.001741 0.001378 0.000656 0.002648 0.000585 0.001373 0.001210 0.001848 0.001227 0.001375 0.000741 0.002617
Cell Periphery 0.000394 0.000208 0.000037 0.000080 0.000111 0.000052 0.000028 0.000138 0.000268 0.000119 0.000035 0.000082
Cytoplasm 0.004463 0.005380 0.000046 0.000484 0.000228 0.000282 0.000150 0.001634 0.002578 0.002461 0.000062 0.000528
Cytoplasmic Foci 0.001146 0.000422 0.000003 0.000358 0.000025 0.000571 0.000018 0.013821 0.000647 0.000508 0.000005 0.000872
Eisosomes 0.000026 0.000037 0.000057 0.000036 0.000040 0.000044 0.000061 0.000047 0.000032 0.000041 0.000058 0.000036
Endoplasmic Reticulum 0.002771 0.003920 0.001023 0.002112 0.003227 0.003176 0.002665 0.001794 0.002974 0.003494 0.001276 0.002100
Endosome 0.000380 0.000298 0.000023 0.000675 0.000119 0.000624 0.000055 0.000656 0.000264 0.000485 0.000028 0.000675
Golgi 0.000090 0.000501 0.000034 0.000260 0.000033 0.000425 0.000071 0.006349 0.000065 0.000457 0.000040 0.000493
Lipid Particles 0.000251 0.000188 0.000004 0.000205 0.000011 0.000458 0.000048 0.000639 0.000144 0.000343 0.000011 0.000222
Mitochondria 0.000778 0.001468 0.000781 0.003198 0.000463 0.001035 0.001139 0.003083 0.000638 0.001220 0.000836 0.003194
Mitotic Spindle 0.000556 0.000463 0.000102 0.004663 0.000743 0.002685 0.000263 0.003019 0.000639 0.001735 0.000126 0.004600
None 0.024193 0.010922 0.000400 0.001442 0.001100 0.001135 0.001626 0.021303 0.013915 0.005318 0.000589 0.002200
Nuclear Periphery 0.000508 0.000151 0.000078 0.000917 0.000173 0.000513 0.000088 0.000597 0.000359 0.000358 0.000079 0.000905
Nuclear Periphery Foci 0.000575 0.000582 0.000006 0.000248 0.000029 0.000454 0.000038 0.000526 0.000332 0.000509 0.000011 0.000259
Nucleolus 0.065153 0.038300 0.019392 0.012308 0.020620 0.030652 0.024302 0.026682 0.045333 0.033921 0.020150 0.012857
Nucleus 0.892226* 0.926781* 0.956490* 0.942154* 0.963296* 0.945178* 0.953021* 0.894614* 0.923858* 0.937314* 0.955955* 0.940339*
Peroxisomes 0.000304 0.000952 0.000189 0.000324 0.000120 0.000484 0.000930 0.000834 0.000222 0.000684 0.000303 0.000343
Vacuole 0.000546 0.000766 0.000146 0.001759 0.000168 0.000425 0.000654 0.003524 0.000378 0.000571 0.000225 0.001826
Vacuole Periphery 0.000322 0.000286 0.000121 0.000735 0.000110 0.000356 0.000103 0.002770 0.000228 0.000326 0.000118 0.000812

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.50 -3.15 -9.73 0.40 -10.56 -7.23 -13.73 -7.52 -5.68 -1.34 -9.39 -9.36 -13.68 -4.12 -8.15
Bud Neck -4.86 -20.39 -17.53 -15.30 -4.12 -1.34 -4.06 -2.51 -2.19 -1.17 -4.63 -19.04 -16.32 -13.92 -4.90
Bud Site 0.59 1.96 -1.24 -2.21 -3.64 -1.93 -1.85 -2.24 -1.11 -1.16 -0.45 1.38 -2.38 -2.21 -3.54
Cell Periphery 2.56 5.40 4.71 4.41 -4.06 1.70 2.42 -0.32 -1.34 -1.74 3.55 5.84 4.58 2.43 -4.52
Cytoplasm -0.65 5.64 4.80 3.57 -4.35 -0.71 0.77 -1.89 -1.79 -1.98 0.08 5.74 4.12 3.07 -4.60
Cytoplasmic Foci 1.60 2.66 2.12 0.94 -2.29 -2.33 0.45 -1.01 -0.94 -1.01 0.76 2.68 0.58 -0.03 -1.74
Eisosomes -4.46 -6.62 -5.38 0.21 4.44 -0.97 -6.35 -2.00 -1.33 1.71 -3.55 -6.37 -2.62 1.63 5.26
Endoplasmic Reticulum -3.29 5.93 1.78 7.17 -10.10 0.17 1.32 3.38 3.24 1.86 -2.45 9.05 3.67 7.60 -7.52
Endosome 0.98 6.18 -1.06 -1.55 -3.47 -2.15 2.81 -1.12 0.31 -1.29 -1.60 7.03 -1.91 -0.34 -3.54
Golgi -1.45 3.96 -1.63 0.99 -2.31 -2.54 -4.81 -1.04 -0.93 -1.03 -2.55 2.55 -1.85 0.46 -1.98
Lipid Particles 0.70 5.00 0.68 0.26 -1.21 -1.79 -4.39 -1.21 0.11 -1.08 -1.38 4.79 -0.20 0.95 -1.28
Mitochondria -1.71 -0.57 -3.75 -2.24 -3.69 -3.22 -5.91 -3.85 -2.99 -2.83 -2.92 -2.85 -4.18 -3.03 -3.75
Mitotic Spindle 0.34 1.48 -3.98 -4.13 -4.45 -1.90 0.94 -1.36 -1.05 -1.45 -1.85 1.75 -4.12 -3.09 -4.64
None 4.62 9.60 9.09 6.40 -3.62 -0.35 -0.84 -1.21 -1.20 -1.17 5.58 9.52 7.93 3.99 -2.65
Nuclear Periphery 1.87 2.21 -1.48 -3.79 -4.11 -1.17 2.06 -1.56 -0.37 -1.78 0.00 2.51 -2.49 -2.16 -4.21
Nuclear Periphery Foci 0.15 2.62 1.67 1.86 -3.93 -1.81 -0.38 -1.59 -0.13 -1.57 -0.69 2.65 0.91 1.66 -4.11
Nucleolus 9.06 17.24 19.83 11.85 5.02 -5.23 -1.50 -1.23 -0.14 -0.86 5.97 13.99 17.09 12.46 4.98
Nucleus -6.26 -14.00 -8.50 -2.16 5.09 5.59 3.57 2.86 2.12 2.39 -3.84 -10.56 -2.71 0.52 5.53
Peroxisomes -2.11 2.43 -0.32 2.01 -1.70 -5.24 -10.42 -4.17 -2.02 -0.12 -3.34 -3.69 -1.89 2.29 -0.49
Vacuole -1.07 2.50 -2.31 -1.85 -3.10 -2.18 -4.43 -1.62 -1.54 -1.42 -1.59 1.19 -2.97 -2.58 -3.20
Vacuole Periphery 0.39 2.52 -1.74 -2.07 -3.10 -3.41 0.06 -1.07 -0.95 -1.07 -1.69 2.28 -2.62 -2.04 -3.26
Standard name
Human Ortholog
Description Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Tfb1

Tfb1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available