Standard name
Human Ortholog
Description Protein involved in nucleotide excision repair (NER); homologous to RAD4
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.07 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.14 0.2 0.42 0.39 0.39 0.36 0.38 0.41 0.29 0.36 0.34 0.34 0.17 0.26 0.29 0.36 0.34 0.35 0.09 0.12 0.14 0.09 0.09 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.05 0.17 0.09 0.11 0 0 0 0 0.05 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.46 0.42 0 0.2 0.11 0.35 0.41 0.26 0.65 0.51 0.59 0.52 0.32 0.12 0.11 0.07 0 0.08 0.05 0 0 0.05 0.09 0.1
Nucleus 0.34 0.38 0.45 0.35 0.39 0.33 0.24 0.22 0.21 0.22 0.15 0.18 0.51 0.46 0.39 0.17 0.21 0.2 0.32 0.42 0.28 0.25 0.13 0.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.1 0.05 0 0.06 0.06 0 0 0 0.05 0 0 0 0 0 0 0.1 0.06 0.05 0.05 0.09 0.1
Vac/Vac Membrane 0.31 0.16 0 0 0.09 0.06 0 0.11 0 0 0.05 0.05 0.13 0.14 0.23 0.41 0.42 0.39 0.31 0.26 0.38 0.42 0.43 0.41
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 2
Bud 0 0 0 1 3 2 8 3 9 8 13 23 5 1 1 0 0 0 1 3 4 6 13 11
Bud Neck 0 0 18 10 21 6 20 26 5 10 5 6 0 0 0 0 0 0 0 1 3 6 14 14
Bud Site 0 0 0 1 2 4 3 3 5 6 9 11 2 2 1 0 0 0 – – – – – –
Cell Periphery 0 0 4 0 1 1 1 0 0 0 0 0 0 0 1 0 0 3 0 0 0 0 0 0
Cytoplasm 21 33 109 114 166 193 262 217 163 217 204 193 70 124 168 144 81 83 17 26 56 36 57 64
Endoplasmic Reticulum 3 1 3 0 1 1 2 0 1 0 0 0 1 2 1 19 5 13 1 1 1 3 4 3
Endosome 0 0 0 5 2 5 5 1 1 3 1 3 15 30 27 66 22 27 8 4 3 6 33 41
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 1 2 1 1
Mitochondria 69 70 2 58 45 186 283 136 362 311 359 295 133 60 67 26 6 20 8 5 18 20 59 61
Nucleus 51 63 116 103 164 172 168 116 119 133 92 103 212 224 229 66 50 48 62 89 112 100 90 64
Nuclear Periphery 1 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 0 0 3 1 0 2 4 3
Nucleolus 0 0 0 0 1 0 1 1 1 2 4 5 3 8 6 0 0 1 7 8 10 12 22 18
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0
SpindlePole 2 2 5 29 19 10 42 31 11 19 14 27 3 17 7 4 6 1 18 12 21 21 57 67
Vac/Vac Membrane 46 26 11 10 36 34 27 59 19 24 30 30 54 66 135 163 99 94 59 55 153 166 286 266
Unique Cell Count 149 165 258 292 421 529 687 523 560 605 605 570 417 486 583 395 236 238 196 214 404 397 671 646
Labelled Cell Count 193 195 270 331 461 614 824 593 697 733 732 696 498 534 643 491 269 292 196 214 404 397 671 646


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.7 4.8 4.3 4.9 3.8 4.0 4.3 3.5 3.5 3.4 3.7 4.1 4.2 4.2 5.9 6.2 6.0 3.9 3.8 4.2
Std Deviation (1e-4) 0.5 0.6 0.7 1.1 1.1 0.9 0.9 1.1 0.9 0.9 0.9 0.6 0.7 1.0 0.9 1.3 1.3 1.2 0.9 0.8 1.0
Intensity Change (Log2) – – – -0.15 0.03 -0.33 -0.27 -0.17 -0.47 -0.45 -0.49 -0.37 -0.24 -0.19 -0.19 0.3 0.35 0.31 -0.3 -0.36 -0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 10.5 10.1 9.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9214 -0.8058 -0.8237 -1.2288 -1.4098 -1.0024 -0.8436 -1.0258 -0.9946 -0.9134 -1.1856 -1.0905 -0.8856 -0.7964 -1.0731 -0.3394 -0.9997 -0.962
Actin 0.0077 0.0064 0.0136 0.0021 0.0091 0.0119 0.0145 0.0002 0.0041 0.0063 0.0027 0.0012 0.0173 0.0003 0.014 0.0547 0.0178 0.0047
Bud 0.0006 0.0077 0.0009 0.0001 0.0003 0.001 0.001 0.0003 0.0002 0.0005 0.0005 0.0002 0.0006 0.0002 0.0003 0.0008 0.0028 0.0003
Bud Neck 0.0167 0.0011 0.0071 0.0026 0.0023 0.0089 0.0038 0.0021 0.0014 0.0256 0.0014 0.0078 0.0021 0.003 0.0021 0.004 0.0049 0.0093
Bud Periphery 0.001 0.0018 0.0021 0.0002 0.0004 0.0017 0.0019 0.0004 0.0002 0.0013 0.0008 0.0004 0.0008 0.0002 0.0005 0.001 0.0052 0.0004
Bud Site 0.0095 0.005 0.0058 0.0009 0.0006 0.0021 0.0056 0.0074 0.0019 0.0048 0.0009 0.0018 0.0021 0.0051 0.0032 0.0088 0.0023 0.0008
Cell Periphery 0.0006 0.0003 0.0007 0.0002 0.0001 0.0002 0.0004 0.0003 0.0001 0.0002 0.0001 0.0002 0.0002 0.0004 0.0003 0.0004 0.0004 0.0001
Cytoplasm 0.0986 0.1679 0.1243 0.0594 0.1118 0.1245 0.106 0.1529 0.1413 0.1109 0.1311 0.1243 0.1422 0.2106 0.1956 0.1158 0.1205 0.1676
Cytoplasmic Foci 0.0407 0.0163 0.0212 0.0131 0.0178 0.0103 0.045 0.0118 0.0188 0.0297 0.0349 0.0118 0.0258 0.0096 0.0156 0.0282 0.0161 0.0121
Eisosomes 0.0003 0.0003 0.0003 0.0001 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0004 0.0003 0.0005 0.0001
Endoplasmic Reticulum 0.0113 0.0069 0.0086 0.0088 0.0055 0.0058 0.0085 0.0052 0.0083 0.0067 0.0034 0.0044 0.0084 0.011 0.0133 0.0126 0.0037 0.0043
Endosome 0.0217 0.0148 0.0256 0.0132 0.026 0.0107 0.0541 0.0151 0.0317 0.03 0.0511 0.0187 0.0355 0.0133 0.0234 0.0518 0.0248 0.0199
Golgi 0.002 0.0016 0.0075 0.0013 0.0029 0.0032 0.0124 0.0004 0.0053 0.0137 0.0118 0.0013 0.009 0.0005 0.0042 0.0181 0.0287 0.0059
Lipid Particles 0.0223 0.0063 0.0113 0.0106 0.0053 0.0024 0.0383 0.0038 0.0132 0.0073 0.0597 0.0096 0.0301 0.0051 0.0168 0.0233 0.0264 0.0095
Mitochondria 0.0037 0.0036 0.0047 0.002 0.0027 0.002 0.0183 0.0019 0.0051 0.0187 0.0129 0.0021 0.008 0.0009 0.0155 0.0224 0.0359 0.0021
None 0.3773 0.3841 0.3732 0.2547 0.2382 0.2154 0.2146 0.3514 0.274 0.304 0.2059 0.1282 0.2301 0.274 0.2075 0.1765 0.1688 0.1206
Nuclear Periphery 0.0253 0.0267 0.0358 0.0341 0.024 0.0252 0.0528 0.0262 0.0457 0.0455 0.0259 0.022 0.0305 0.032 0.0474 0.0521 0.0138 0.0249
Nucleolus 0.0101 0.013 0.0172 0.0291 0.0239 0.0147 0.0264 0.0298 0.0427 0.008 0.0115 0.0427 0.01 0.0381 0.0163 0.0068 0.0234 0.0159
Nucleus 0.2606 0.2801 0.2609 0.4951 0.4373 0.4926 0.3213 0.331 0.3336 0.3207 0.2761 0.535 0.3789 0.3436 0.3743 0.3553 0.4341 0.5341
Peroxisomes 0.0052 0.0009 0.0074 0.0033 0.0039 0.0006 0.02 0.0009 0.0037 0.0065 0.011 0.0045 0.0242 0.0003 0.0041 0.0229 0.0099 0.0024
Punctate Nuclear 0.0663 0.0462 0.0589 0.061 0.0814 0.0623 0.0403 0.0401 0.0571 0.0479 0.1531 0.0775 0.0344 0.0411 0.0334 0.0296 0.0511 0.0601
Vacuole 0.0152 0.008 0.0114 0.0069 0.0049 0.0032 0.01 0.0174 0.0096 0.0082 0.0035 0.0055 0.0079 0.0096 0.0098 0.0119 0.0065 0.004
Vacuole Periphery 0.0032 0.001 0.0016 0.0011 0.0015 0.001 0.0046 0.0013 0.0017 0.0035 0.0018 0.0008 0.0015 0.0009 0.002 0.0025 0.0025 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.4498 6.6423 4.2247 6.0318 5.1609 8.4871 8.5149 7.6087 5.4082 8.2393
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Protein involved in nucleotide excision repair (NER); homologous to RAD4
Localization
Cell Percentages nucleus (22%), cytoplasm (12%), mixed (54%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00045)
Subcompartmental Group N/A

Rad34

Rad34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rad34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available