Standard name
Human Ortholog
Description Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; modified by sumoylation; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 – – 0.12 – 0 0 0 0 0.12 0.07 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0.05 – – 0.06 – 0 0.1 0.07 0.05 0.12 0.1 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0.19 – 0 0.06 0.13 0.12 0.06 0.12 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 0.07 0.06 – – 0.06 – 0.08 0.18 0.07 0.14 0.08 0.06 0.15 0.25 0.23 – 0.27 0.28 0 0 0 0 0.05 0.07
Endoplasmic Reticulum 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0
Endosome 0.29 0.05 – – 0 – 0 0 0 0 0 0 0.16 0.15 0.34 – 0.24 0.14 0.1 0.11 0.07 0.08 0.09 0.08
Golgi 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.19 0.26 – – 0.12 – 0.05 0.12 0.43 0.25 0.41 0.3 0 0 0 – 0.06 0.09 0 0 0 0 0 0
Nucleus 0 0 – – 0 – 0.08 0 0.06 0.08 0.05 0 0 0 0 – 0.06 0.06 0 0 0 0 0 0
Nuclear Periphery 0 0 – – 0.06 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0.3 0.06 – – 0.25 – 0.22 0.14 0.19 0.17 0.22 0.12 0.07 0.11 0.09 – 0.12 0.17 0 0.05 0 0 0 0
Peroxisomes 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0.63 0.7 – – 0.75 – 0.76 0.68 0.57 0.68 0.58 0.71 0.74 0.62 0.54 – 0.56 0.57 0.76 0.73 0.76 0.73 0.65 0.62
Vac/Vac Membrane 0.09 0 – – 0 – 0 0 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 – – 0 – 0 1 0 4 0 0 0 0 0 – 0 0 0 2 0 0 0 1
Bud 1 18 – – 2 – 0 1 8 5 15 9 0 2 0 – 3 1 1 1 0 1 2 6
Bud Neck 0 20 – – 1 – 1 5 12 7 16 13 7 5 5 – 2 5 1 5 0 2 3 1
Bud Site 1 9 – – 3 – 1 3 23 16 8 16 0 0 0 – 2 2 – – – – – –
Cell Periphery 0 0 – – 0 – 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 9 27 – – 1 – 3 9 13 19 11 8 24 38 41 – 38 38 3 12 1 5 10 15
Endoplasmic Reticulum 0 1 – – 0 – 0 0 0 0 0 0 0 1 0 – 3 8 0 0 0 0 0 0
Endosome 36 20 – – 0 – 0 2 0 0 1 0 25 23 60 – 34 19 15 57 5 19 15 16
Golgi 0 0 – – 0 – 0 0 0 0 0 0 1 0 0 – 0 1 1 2 0 0 0 0
Mitochondria 24 110 – – 2 – 2 6 78 35 53 38 1 1 1 – 9 12 2 11 2 3 4 8
Nucleus 3 9 – – 0 – 3 1 11 11 6 4 7 3 6 – 8 8 1 3 1 8 5 4
Nuclear Periphery 0 0 – – 1 – 0 0 0 0 0 0 1 0 0 – 0 1 0 0 0 0 1 0
Nucleolus 38 27 – – 4 – 8 7 35 24 28 16 11 16 15 – 17 23 2 22 1 9 5 4
Peroxisomes 1 2 – – 0 – 1 0 4 1 0 0 1 0 3 – 0 0 0 2 0 0 0 0
SpindlePole 79 290 – – 12 – 28 34 103 94 76 91 117 94 95 – 78 78 111 363 61 169 121 126
Vac/Vac Membrane 11 6 – – 0 – 0 0 1 0 0 0 1 1 7 – 6 7 3 4 2 5 7 10
Unique Cell Count 126 417 16 37 50 180 139 130 128 159 152 175 139 136 148 501 80 232 186 203
Labelled Cell Count 204 542 26 47 69 288 216 214 195 196 184 233 200 203 148 501 80 232 186 203


Nucleus, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 3.5 – – 3.9 – 3.9 4.7 3.3 4.0 3.4 3.4 5.2 5.0 5.0 4.5 5.9 5.9 5.4 6.0 6.0
Std Deviation (1e-4) 0.7 0.6 – – 1.4 – 1.2 2.1 0.8 1.9 0.8 0.5 1.1 0.7 1.4 1.3 2.2 1.9 1.2 1.5 1.8
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2814 -0.9246 -0.9706 -1.1374 -1.2069 -0.9683 4.7497 3.9832 4.5175 4.5338 5.3301 4.435 1.5989 1.3128 1.4097 1.6405 1.5508 1.4348
Actin 0.002 0.0011 0.0009 0.0003 0.0003 0.0047 0.0306 0.0109 0.0193 0.0032 0.0135 0.0052 0.0025 0.0017 0.0079 0.0006 0.0027 0.0013
Bud 0.0001 0.0001 0 0 0 0 0.0011 0.0003 0.0002 0.0001 0.0011 0.0002 0.0001 0.0002 0.0002 0.0001 0.0004 0
Bud Neck 0.0025 0.0016 0.0103 0.0142 0.0007 0.0008 0.0111 0.0016 0.0009 0.0006 0.0024 0.001 0.0022 0.001 0.0031 0.0124 0.0003 0.0022
Bud Periphery 0.0001 0.0001 0 0 0 0.0001 0.0023 0.0003 0.0002 0.0001 0.0013 0.0003 0.0002 0.0006 0.0003 0.0001 0.0013 0
Bud Site 0.0044 0.0022 0.0009 0.0001 0.0001 0 0.0064 0.0051 0.0013 0.0002 0.0147 0.0002 0.0036 0.0027 0.0024 0.0003 0.0003 0.0002
Cell Periphery 0.0003 0.0001 0 0 0 0 0.0005 0 0 0 0.0008 0 0.0002 0.0001 0.0001 0 0.0001 0
Cytoplasm 0.0098 0.0392 0.0048 0.001 0.0021 0.0106 0.012 0.0378 0.0066 0.006 0.0076 0.0109 0.0199 0.0336 0.0169 0.011 0.0021 0.0078
Cytoplasmic Foci 0.1317 0.1005 0.1094 0.1248 0.1959 0.0809 0.1104 0.1298 0.121 0.1232 0.2366 0.0766 0.0792 0.0581 0.0523 0.0871 0.2441 0.0475
Eisosomes 0.0003 0.0002 0.0001 0 0.0001 0.0001 0.0004 0 0.0001 0.0001 0.0002 0.0002 0.0001 0.0002 0.0001 0 0.0001 0.0001
Endoplasmic Reticulum 0.0009 0.0016 0.0003 0 0.0001 0.0011 0.0114 0.0006 0.0003 0.0005 0.0032 0.0005 0.0027 0.0008 0.0007 0.0004 0.0014 0.0004
Endosome 0.009 0.0078 0.0115 0.0012 0.0026 0.0035 0.0277 0.0073 0.0081 0.0075 0.0889 0.0068 0.0255 0.0071 0.0124 0.0368 0.0282 0.0079
Golgi 0.0022 0.0014 0.0013 0.0007 0.0015 0.0018 0.0142 0.0014 0.0085 0.0045 0.0225 0.0047 0.0057 0.0014 0.0069 0.0079 0.0108 0.0017
Lipid Particles 0.0274 0.0273 0.0228 0.0042 0.0781 0.0118 0.0394 0.0066 0.0117 0.0546 0.1502 0.0182 0.0334 0.0187 0.0148 0.0251 0.0514 0.0134
Mitochondria 0.0003 0.0002 0.0003 0.0001 0.0001 0.0004 0.0066 0.0004 0.0078 0.0007 0.006 0.0062 0.0036 0.0018 0.0178 0.0029 0.0136 0.0008
None 0.019 0.1403 0.0521 0.0056 0.0633 0.0634 0.0386 0.1173 0.07 0.0889 0.0586 0.061 0.0815 0.1851 0.0989 0.0311 0.0453 0.0786
Nuclear Periphery 0.007 0.004 0.0043 0.0012 0.0015 0.004 0.0158 0.0024 0.005 0.0038 0.0092 0.0041 0.0029 0.0094 0.0059 0.0114 0.0038 0.0032
Nucleolus 0.0042 0.0301 0.0103 0.0023 0.0392 0.0068 0.0066 0.0128 0.0053 0.0017 0.0022 0.0022 0.0141 0.0131 0.0137 0.0042 0.001 0.0103
Nucleus 0.0226 0.0528 0.0358 0.0021 0.0239 0.0166 0.0049 0.0283 0.005 0.0032 0.0021 0.0085 0.0309 0.0447 0.0086 0.0122 0.0006 0.0412
Peroxisomes 0.0109 0.0116 0.0113 0.0044 0.0126 0.0033 0.0153 0.0109 0.0133 0.0392 0.0208 0.0059 0.0326 0.0099 0.0106 0.0153 0.0475 0.0128
Punctate Nuclear 0.745 0.5768 0.7226 0.8379 0.5779 0.7899 0.6417 0.6254 0.7147 0.6616 0.3493 0.7866 0.6545 0.6066 0.7251 0.7399 0.543 0.7694
Vacuole 0.0005 0.0009 0.0006 0 0.0002 0.0002 0.0017 0.0006 0.0004 0.0002 0.0054 0.0004 0.0037 0.0026 0.001 0.0008 0.0011 0.0008
Vacuole Periphery 0.0002 0.0001 0.0002 0 0 0.0002 0.0013 0.0001 0.0002 0.0001 0.0034 0.0003 0.0009 0.0006 0.0004 0.0005 0.001 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression – – – – – – – – – –
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression –
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1697 608 2087 1963 1548 2228 481 1738 3245 2836 2568 3701

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 636.92 764.61 800.93 828.78 674.50 824.34 815.70 818.61 654.85 811.53 803.70 824.00
Standard Deviation 71.34 97.93 92.52 112.91 82.04 93.61 106.14 115.87 78.89 97.68 95.39 114.42
Intensity Change Log 2 — 0.263612 0.330564 0.379877 — 0.289421 0.274220 0.279358 — 0.276944 0.301860 0.329052

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000257 0.000594 0.000366 0.001290 0.000287 0.000277 0.000849 0.000638 0.000271 0.000345 0.000457 0.000984
Bud Neck 0.009464 0.011132 0.006503 0.010014 0.005402 0.017970 0.003972 0.004508 0.007526 0.016504 0.006029 0.007428
Bud Site 0.002732 0.009669 0.008191 0.030039 0.003309 0.008579 0.005213 0.026707 0.003007 0.008813 0.007633 0.028474
Cell Periphery 0.000319 0.000328 0.000132 0.000092 0.000228 0.000210 0.000411 0.000206 0.000276 0.000236 0.000185 0.000146
Cytoplasm 0.103584* 0.111854* 0.105009* 0.083685 0.237496* 0.120496* 0.184656* 0.123843* 0.167466* 0.118643* 0.119927* 0.102543*
Cytoplasmic Foci 0.369890* 0.216924* 0.013628 0.014707 0.262225* 0.267821* 0.028109 0.016249 0.318529* 0.256909* 0.016340 0.015431
Eisosomes 0.000175 0.000100 0.000048 0.000029 0.000107 0.000101 0.000105 0.000028 0.000142 0.000101 0.000058 0.000029
Endoplasmic Reticulum 0.000557 0.001254 0.001005 0.001036 0.001647 0.000493 0.002076 0.000880 0.001077 0.000656 0.001206 0.000962
Endosome 0.011413 0.027693 0.004236 0.011818 0.014479 0.023700 0.007561 0.005813 0.012875 0.024556 0.004859 0.008998
Golgi 0.017749 0.021178 0.000567 0.009163 0.006621 0.015222 0.000861 0.002733 0.012441 0.016499 0.000622 0.006143
Lipid Particles 0.075196 0.032495 0.002357 0.001881 0.024268 0.039409 0.007630 0.001427 0.050901 0.037927 0.003345 0.001668
Mitochondria 0.019880 0.011966 0.001098 0.007503 0.004626 0.018638 0.003099 0.001289 0.012603 0.017208 0.001473 0.004585
Mitotic Spindle 0.051769 0.329347* 0.553843* 0.724210* 0.088451 0.242665* 0.164592* 0.674363* 0.069268 0.261249* 0.480935* 0.700801*
None 0.049485 0.013167 0.014928 0.003639 0.048425 0.016390 0.043734 0.014583 0.048979 0.015699 0.020323 0.008778
Nuclear Periphery 0.000871 0.001042 0.000846 0.001458 0.000958 0.000934 0.001162 0.000749 0.000913 0.000957 0.000905 0.001125
Nuclear Periphery Foci 0.041008 0.020243 0.011232 0.005931 0.050481 0.013885 0.010593 0.016250 0.045527 0.015248 0.011112 0.010777
Nucleolus 0.122417 0.054650 0.037148 0.008356 0.104038 0.067490 0.044305 0.015456 0.113650 0.064738 0.038488 0.011690
Nucleus 0.077185 0.075305 0.230037* 0.055594 0.125147 0.086092 0.466886* 0.075426 0.100065 0.083780 0.274400* 0.064907
Peroxisomes 0.043528 0.054189 0.002455 0.003618 0.017425 0.052770 0.005266 0.005752 0.031075 0.053074 0.002982 0.004620
Vacuole 0.001831 0.005688 0.006100 0.022119 0.003828 0.006029 0.018491 0.011001 0.002784 0.005956 0.008421 0.016898
Vacuole Periphery 0.000692 0.001179 0.000271 0.003818 0.000551 0.000827 0.000427 0.002100 0.000625 0.000902 0.000300 0.003011

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.21 -10.05 -8.41 -3.03 -4.68 4.73 -9.04 -11.06 -13.03 4.79 -2.66 -12.85 -11.75 -10.20 -3.08
Bud Neck -3.57 -6.21 -12.57 -4.99 -8.41 -7.29 -7.69 -10.50 -2.14 -0.41 -7.55 -9.67 -16.18 -6.17 -7.53
Bud Site -6.16 -9.20 -12.92 -8.40 -7.90 -1.94 -8.42 -10.03 -9.06 -2.48 -5.43 -11.09 -16.33 -13.47 -8.62
Cell Periphery -0.76 6.95 7.18 1.94 0.18 3.10 -4.20 1.97 -0.49 4.81 1.37 4.02 6.45 1.77 2.30
Cytoplasm -3.92 1.08 -6.92 -0.51 -7.96 3.36 0.22 0.88 -2.39 0.39 -0.60 3.65 -3.82 -3.07 -7.25
Cytoplasmic Foci 9.93 41.51 41.27 19.52 -0.39 -3.55 26.50 33.07 43.26 2.66 2.68 51.50 52.51 47.83 0.66
Eisosomes 4.93 15.85 15.44 6.66 -0.06 7.13 -3.95 12.78 7.24 9.09 10.51 12.31 20.01 10.19 5.00
Endoplasmic Reticulum -5.75 -11.74 -14.74 -7.88 -5.45 5.01 -10.02 -8.27 -15.15 6.34 2.71 -13.11 -16.17 -19.27 -1.40
Endosome -6.96 8.81 3.17 7.90 -4.51 -3.09 4.42 8.20 11.92 2.18 -8.42 11.86 8.27 14.36 -3.53
Golgi 0.30 17.89 13.40 7.32 -1.55 0.12 18.13 19.87 13.75 2.61 2.43 24.88 19.67 13.75 -1.36
Lipid Particles 6.71 20.09 19.94 10.52 -1.64 -6.57 9.73 14.59 19.83 4.09 2.95 23.15 23.56 22.35 1.82
Mitochondria 0.03 5.15 3.27 3.41 -4.05 -6.45 0.82 4.97 7.95 1.93 -2.80 5.75 3.97 7.45 -3.49
Mitotic Spindle -15.87* -51.30* -48.57* -14.82 1.69 -7.04 -8.85 -38.42* -32.96* -13.80 -16.85 -47.24 -61.20 -38.73* -3.80
None 9.54 13.52 14.89 3.96 2.55 9.59 3.54 12.93 5.31 4.47 13.46 16.07 19.75 8.43 3.59
Nuclear Periphery -1.67 -5.52 -16.08 -12.25 -13.48 -4.10 -8.86 -11.32 -9.24 -0.13 -3.61 -10.16 -19.57 -17.03 -12.65
Nuclear Periphery Foci 6.61 12.21 18.87* 6.86 8.38 11.17 6.09 10.72 0.05 1.84 14.50 14.69 20.78 6.03 5.34
Nucleolus 11.78 14.02 22.61* 5.38 9.67 5.40 7.70 21.67 17.98 6.98 9.17 17.60 31.33* 19.97 11.61
Nucleus -2.68 -13.03 -14.45 -8.49 -0.82 0.82 -13.73 -8.63 -9.46 10.22 -1.41 -17.53 -16.41 -14.47 5.01
Peroxisomes -2.97 19.87 18.43 12.02 -1.40 -7.06 10.02 15.83 22.33 2.52 -5.97 24.75 24.17 25.79 -0.39
Vacuole -5.25 -7.64 -14.61 -12.91 -12.78 -2.12 -7.14 -10.75 -9.86 0.42 -4.51 -10.07 -17.82 -16.41 -12.67
Vacuole Periphery -3.82 0.63 -2.80 -1.02 -3.06 -1.76 -4.32 -1.31 -0.92 -0.59 -2.88 -0.29 -2.95 -2.03 -2.95
Standard name
Human Ortholog
Description Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; modified by sumoylation; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O and fission yeast mal2
Localization
Cell Percentages spindle pole (42%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mcm21

Mcm21


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mcm21-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available