Standard name
Human Ortholog
Description Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.92 0.91 0.99 0.98 0.97 0.96 0.94 0.92 0.81 0.85 0.7 0.78 0.98 0.95 0.88 0.87 0.88 0.79 0.82 0.76 0.76 0.71 0.78 0.73
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.11 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.12 0 0 0 0 0.06 0 0.28 0.17 0.37 0.26 0 0 0 0 0 0 0.08 0.05 0.12 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0.15 0.13 0.18 0.07 0.13 0.05 0.09 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 1 0 1 0 0 0 0 0 1 0 0 0 0 0 2 0 0 2
Bud 0 1 0 2 0 0 4 3 10 8 7 8 0 1 3 0 0 0 0 0 0 0 5 4
Bud Neck 1 0 9 4 10 10 18 13 6 8 1 5 2 1 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 2 3 1 2 0 0 0 1 3 3 0 0 1 0 0 0 1 0 0 0
Cytoplasm 85 53 268 319 401 341 449 456 289 370 129 191 148 300 346 54 155 124 95 58 192 65 135 156
Endoplasmic Reticulum 2 0 0 0 1 2 0 0 0 0 0 0 0 4 2 1 10 5 0 1 1 0 1 1
Endosome 0 0 0 1 1 1 1 2 1 6 2 6 1 4 19 1 5 17 1 0 4 3 1 4
Golgi 0 0 0 1 0 0 1 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1
Mitochondria 6 7 0 2 1 12 28 18 99 76 67 64 0 0 2 2 4 3 8 3 29 4 4 8
Nucleus 0 1 3 2 2 0 4 5 4 2 1 1 0 2 2 0 0 1 0 0 1 3 6 5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1
Peroxisomes 0 0 0 0 0 1 0 1 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 2 2 10 15 5 15 2 5 0 0 0 0 0 0 0 0 1 0 1 6
Vac/Vac Membrane 1 2 2 3 2 1 2 12 7 8 2 7 0 9 38 9 23 28 8 9 12 8 7 14
Unique Cell Count 92 58 270 326 414 357 478 493 358 437 183 244 151 317 394 62 177 156 117 77 253 92 174 216
Labelled Cell Count 95 64 282 336 422 374 519 528 423 494 213 288 157 324 414 67 198 178 117 77 253 92 174 216


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 3.3 4.9 4.6 5.3 4.0 4.2 4.8 3.5 4.8 3.7 4.1 4.6 5.0 5.3 5.3 5.4 6.3 5.1 5.6 5.5
Std Deviation (1e-4) 0.4 0.6 1.3 1.6 1.8 1.1 1.6 3.1 1.3 8.7 1.2 1.7 1.2 1.1 1.6 1.9 1.6 2.0 1.7 1.9 1.9
Intensity Change (Log2) – – – -0.1 0.11 -0.27 -0.2 -0.03 -0.48 -0.02 -0.41 -0.25 -0.07 0.04 0.12 0.12 0.15 0.37 0.07 0.2 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 0
Bud Neck – – – – – – – – – – – – – 0 0
Bud Site – – – – – – – – – – – – – 0 0
Cell Periphery – – – – – – – – – – – – – 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 0
Endosome – – – – – – – – – – – – – 0 0
Golgi – – – – – – – – – – – – – 0 0
Mitochondria – – – – – – – – – – – – – 0 0
Nuclear Periphery – – – – – – – – – – – – – 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 0
Peroxisomes – – – – – – – – – – – – – 0 0
SpindlePole – – – – – – – – – – – – – 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 0
Cytoplasm – – – – – – – – – – – – – 0 0
Nucleus – – – – – – – – – – – – – 0 0
Vacuole – – – – – – – – – – – – – 5.5 6.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.5588 0.5116 0.4676 -0.201 0.3793 0.3978 0.745 0.6041 0.9215 1.0187 0.6703 0.8105 -0.9919 -0.6097 -1.0181 -0.6015 -1.4718 -0.7709
Actin 0.0314 0.0003 0.0212 0.0001 0.0021 0.0014 0.0328 0.0002 0.0165 0.0742 0.0084 0.0036 0.0073 0.0002 0.0099 0.0783 0.0141 0.006
Bud 0.0011 0.0127 0.0029 0.0003 0.0006 0.0001 0.0005 0.007 0.0009 0.0017 0.0014 0.0005 0.0003 0.0013 0.0001 0.0004 0.0002 0.0038
Bud Neck 0.0005 0.0002 0.0035 0.0001 0.0476 0.0002 0.0009 0.0002 0.0005 0.0006 0.001 0.0031 0.001 0.0001 0.0001 0.0012 0.0003 0.0085
Bud Periphery 0.0034 0.0124 0.0054 0.0004 0.0011 0.0002 0.001 0.0078 0.0018 0.0093 0.0045 0.0018 0.0003 0.0007 0.0001 0.0006 0.0004 0.0079
Bud Site 0.0031 0.0044 0.0253 0.0001 0.0022 0 0.0034 0.0042 0.0029 0.0016 0.0014 0.0002 0.0026 0.0008 0.0003 0.0021 0.0009 0.001
Cell Periphery 0.0005 0.0003 0.0008 0.0001 0.0004 0 0.0004 0.0004 0.0004 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0001 0.0006
Cytoplasm 0.0476 0.1077 0.0548 0.2161 0.0161 0.0969 0.0609 0.0901 0.0929 0.0761 0.0612 0.1103 0.1318 0.2578 0.1647 0.1233 0.1423 0.2728
Cytoplasmic Foci 0.0341 0.0092 0.0191 0.0139 0.025 0.0236 0.0259 0.0084 0.0155 0.0645 0.05 0.0095 0.0195 0.0166 0.0115 0.0218 0.0073 0.0216
Eisosomes 0.001 0.0002 0.0005 0.0001 0.0002 0.0001 0.001 0.0003 0.0004 0.0006 0.0002 0.0001 0.0002 0 0.0001 0.0005 0.0002 0.0001
Endoplasmic Reticulum 0.0038 0.0008 0.0018 0.0012 0.0003 0.0008 0.0024 0.0021 0.0043 0.0011 0.0005 0.0007 0.0057 0.003 0.0021 0.0031 0.0019 0.0093
Endosome 0.0229 0.013 0.0359 0.0064 0.0164 0.035 0.0244 0.0109 0.0314 0.0259 0.096 0.0112 0.0469 0.0333 0.0529 0.0365 0.0137 0.0213
Golgi 0.0098 0.0002 0.0028 0.0002 0.0066 0.0009 0.0066 0.0003 0.0069 0.0174 0.023 0.0039 0.0024 0.0007 0.0011 0.0086 0.0069 0.001
Lipid Particles 0.0146 0.0003 0.0044 0.0002 0.0378 0.0037 0.0139 0.0011 0.0061 0.0238 0.0144 0.0024 0.004 0.0005 0.0027 0.0172 0.0218 0.0011
Mitochondria 0.0166 0.0002 0.003 0.0003 0.0088 0.0017 0.0106 0.0005 0.0126 0.0173 0.0183 0.0023 0.0012 0.001 0.0031 0.0039 0.0468 0.0009
None 0.7661 0.8287 0.8079 0.7472 0.8029 0.8213 0.7632 0.8503 0.785 0.6115 0.6823 0.8373 0.7466 0.6755 0.731 0.6582 0.705 0.6174
Nuclear Periphery 0.0016 0.0002 0.0008 0.0007 0.0003 0.0004 0.0133 0.0007 0.0049 0.001 0.0004 0.0005 0.0038 0.0011 0.0006 0.0075 0.0008 0.002
Nucleolus 0.0008 0.0003 0.0006 0.0001 0.0013 0.0001 0.0022 0.0004 0.0009 0.0008 0.0004 0.0002 0.0013 0.0002 0.0001 0.0006 0.0002 0.0009
Nucleus 0.0008 0.0005 0.0007 0.001 0.0006 0.0004 0.006 0.0009 0.0016 0.001 0.0004 0.0005 0.0079 0.0023 0.0003 0.006 0.0007 0.002
Peroxisomes 0.0263 0.0001 0.0023 0.0001 0.0257 0.0067 0.0156 0.0003 0.0035 0.0598 0.0247 0.005 0.0014 0.0001 0.0102 0.0169 0.0269 0.0007
Punctate Nuclear 0.0084 0.0005 0.0008 0.0002 0.0008 0.001 0.0085 0.0005 0.0015 0.002 0.0035 0.0015 0.0072 0.0003 0.0002 0.0025 0.0002 0.0151
Vacuole 0.0044 0.0074 0.0037 0.0101 0.0025 0.0042 0.0049 0.0125 0.0079 0.0084 0.0056 0.0047 0.0077 0.0038 0.0063 0.0088 0.008 0.0047
Vacuole Periphery 0.0011 0.0004 0.0019 0.0013 0.0006 0.0014 0.0015 0.0009 0.0016 0.0013 0.0023 0.0006 0.0009 0.0008 0.0024 0.0016 0.0012 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.0248 11.9561 3.5488 13.6981 7.8033 1.3367 12.9954 15.5023 15.5453 16.4255
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1979 682 2242 1467 1711 1582 2835 1662 3690 2264 5077 3129

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 642.45 741.07 831.09 778.47 740.92 751.29 751.55 799.91 688.11 748.21 786.67 789.86
Standard Deviation 76.08 88.36 114.20 128.34 136.36 84.47 98.05 115.57 118.90 85.79 112.64 122.19
Intensity Change Log 2 — 0.206026 0.371421 0.277057 — 0.020052 0.020551 0.110520 — 0.109409 0.194146 0.190258

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000308 0.000900 0.000239 0.000619 0.000489 0.000244 0.000098 0.000803 0.000392 0.000442 0.000160 0.000717
Bud Neck 0.030476 0.047626 0.018572 0.011392 0.010727 0.043022 0.015081 0.008442 0.021319 0.044409 0.016623 0.009825
Bud Site 0.006770 0.025408 0.017148 0.027454 0.020579 0.013440 0.009944 0.030595 0.013173 0.017045 0.013125 0.029122
Cell Periphery 0.000213 0.000196 0.000084 0.000277 0.000208 0.000142 0.000449 0.000175 0.000211 0.000159 0.000288 0.000223
Cytoplasm 0.335262* 0.335500* 0.502061* 0.436136* 0.279032* 0.439004* 0.477590* 0.518417* 0.309189* 0.407825* 0.488396* 0.479841*
Cytoplasmic Foci 0.346402* 0.294407* 0.041701 0.053749 0.281147* 0.241562* 0.036467 0.053115 0.316144* 0.257481* 0.038778 0.053412
Eisosomes 0.000562 0.000192 0.000020 0.000074 0.000091 0.000167 0.000161 0.000052 0.000343 0.000174 0.000099 0.000062
Endoplasmic Reticulum 0.001348 0.001570 0.002107 0.002029 0.005799 0.000492 0.001688 0.003242 0.003412 0.000817 0.001873 0.002674
Endosome 0.034307 0.030484 0.018509 0.024465 0.114498 0.017491 0.017049 0.013940 0.071491 0.021405 0.017694 0.018874
Golgi 0.013375 0.006354 0.001611 0.003421 0.080676 0.005085 0.000937 0.005210 0.044581 0.005467 0.001235 0.004371
Lipid Particles 0.022220 0.007751 0.002518 0.003647 0.012070 0.006140 0.003706 0.002717 0.017514 0.006625 0.003181 0.003153
Mitochondria 0.017302 0.015894 0.002861 0.002308 0.018189 0.010063 0.001559 0.003076 0.017713 0.011820 0.002134 0.002716
Mitotic Spindle 0.000238 0.008309 0.025373 0.031385 0.006861 0.006401 0.011020 0.014124 0.003309 0.006976 0.017358 0.022217
None 0.001436 0.001350 0.002524 0.005480 0.004263 0.002378 0.005606 0.002669 0.002747 0.002068 0.004245 0.003987
Nuclear Periphery 0.000291 0.000473 0.003010 0.001365 0.000853 0.000324 0.002674 0.001554 0.000551 0.000369 0.002823 0.001465
Nuclear Periphery Foci 0.000193 0.000594 0.001119 0.002044 0.001306 0.000185 0.000705 0.001666 0.000709 0.000309 0.000888 0.001843
Nucleolus 0.001273 0.001881 0.001075 0.001212 0.001474 0.001573 0.001672 0.000432 0.001366 0.001666 0.001408 0.000797
Nucleus 0.098367 0.109855 0.240387* 0.210276* 0.034149 0.129410 0.277640* 0.186251* 0.068590 0.123519 0.261189* 0.197514*
Peroxisomes 0.015402 0.021191 0.002049 0.003107 0.005231 0.014287 0.001318 0.001916 0.010686 0.016366 0.001641 0.002475
Vacuole 0.069165 0.086686 0.114913 0.176069 0.095048 0.066869 0.132926 0.150052 0.081167 0.072838 0.124972 0.162250
Vacuole Periphery 0.005090 0.003379 0.002117 0.003490 0.027310 0.001721 0.001709 0.001553 0.015394 0.002220 0.001889 0.002461

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.95 0.92 -1.80 1.09 -2.42 3.60 5.97 -0.81 -2.71 -3.84 -0.61 4.49 -1.96 -1.51 -4.43
Bud Neck -4.01 5.58 11.76 9.53 6.05 -11.87 -3.96 2.93 13.08 6.67 -9.74 3.46 11.91 15.99 8.90
Bud Site -6.29 -4.82 -5.89 1.25 -2.46 4.13 7.09 -0.20 -3.20 -5.17 -2.59 1.84 -4.47 -2.07 -5.52
Cell Periphery 0.92 10.87 -0.22 -0.86 -5.77 3.93 -1.80 3.41 0.03 2.60 4.45 -0.57 2.12 -1.36 1.24
Cytoplasm 0.04 -16.64 -6.06 -4.49 8.00 -15.32 -19.76 -17.16 -1.77 0.69 -12.82 -25.59 -16.72 -2.47 5.97
Cytoplasmic Foci 5.38 57.18 54.92 27.04 -1.79 5.08 45.51 42.10 33.16 -3.51 9.86 72.69 68.45 42.67 -3.81
Eisosomes 7.33 11.11 10.06 7.81 -15.56 -8.62 -2.08 8.42 13.73 3.83 6.24 8.98 10.60 15.12 1.86
Endoplasmic Reticulum -0.62 -4.93 -3.26 -1.70 0.20 7.75 5.80 3.39 -6.39 -3.16 7.49 3.95 1.59 -6.95 -2.56
Endosome 1.44 9.66 8.31 4.72 -0.39 26.97* 27.58 29.52 5.66 4.10 22.66* 26.99 27.89 6.38 2.45
Golgi 5.80 13.06 11.46 4.37 -1.52 19.22* 20.58 19.73 2.20 -2.69 19.47 22.40 21.19 4.25 -3.12
Lipid Particles 10.76 18.81 17.91 5.17 -1.58 6.12 9.49 10.39 6.91 2.14 13.70 20.36 20.46 8.64 0.97
Mitochondria 0.73 10.10 10.75 6.52 1.91 4.62 12.34 11.08 6.01 -0.86 4.28 15.77 15.26 8.89 0.34
Mitotic Spindle -3.63 -10.36 -8.15 -4.02 -0.38 0.36 -2.73 -3.42 -3.40 -1.30 -3.17 -10.13 -8.73 -5.69 -1.24
None 0.46 -3.84 -3.33 -3.54 -2.09 2.59 -0.72 1.87 -0.82 2.78 1.79 -2.16 -1.11 -2.80 0.90
Nuclear Periphery -2.63 -16.24 -16.37 -11.69 7.56 1.83 -6.20 -3.93 -11.38 3.60 1.25 -13.76 -9.29 -16.89 7.25
Nuclear Periphery Foci -2.40 -8.40 -5.64 -3.64 -1.49 4.14 1.50 -1.08 -7.44 -3.27 2.90 -2.12 -4.84 -7.98 -3.36
Nucleolus -0.98 0.07 0.66 1.99 1.62 -0.40 -1.39 3.75 3.11 8.45 -0.88 -0.86 2.46 3.33 7.51
Nucleus -1.85 -27.38 -17.62 -12.26 5.95 -20.15 -47.32 -30.98 -12.36 10.05 -14.08 -51.16 -32.94 -17.10 11.46
Peroxisomes -2.66 14.31 13.60 9.63 -0.77 -8.01 10.77 10.03 11.93 -0.59 -5.23 17.08 16.21 15.08 -1.04
Vacuole -3.95 -14.22 -24.57 -18.81 -14.57 5.57 -9.98 -17.77 -22.20 -9.82 1.31 -17.44 -30.34 -30.13 -17.18
Vacuole Periphery 1.96 4.46 3.48 1.77 -0.80 15.75 15.95 16.26 2.80 2.20 14.78 15.81 15.53 2.27 0.34
Standard name
Human Ortholog
Description Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens
Localization
Cell Percentages cytoplasm (11%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Yft2

Yft2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yft2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available