Standard name | |
---|---|
Human Ortholog | |
Description | Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 14 | 32 | 7 | 12 | 10 | 17 | 17 | – | 10 | 19 | 9 | 10 | 17 | 19 | 6 | 29 | 24 | 20 | 7 | 22 | 5 | 6 | 9 | 9 |
Bud | 2 | 2 | 1 | 2 | 1 | 0 | 3 | – | 12 | 9 | 19 | 9 | 0 | 1 | 0 | 1 | 3 | 5 | 2 | 9 | 0 | 2 | 2 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 3 | 1 | 2 | 3 | – | 0 | 3 | 1 | 4 | 0 | 1 | 2 | 4 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 39 | 57 | 24 | 15 | 25 | 53 | 77 | – | 45 | 50 | 35 | 72 | 83 | 77 | 121 | 58 | 49 | 54 | 11 | 36 | 3 | 14 | 31 | 47 |
Endoplasmic Reticulum | 23 | 56 | 8 | 20 | 14 | 22 | 34 | – | 15 | 23 | 23 | 28 | 34 | 28 | 19 | 12 | 11 | 15 | 7 | 21 | 3 | 3 | 13 | 22 |
Endosome | 13 | 11 | 12 | 10 | 25 | 14 | 26 | – | 18 | 8 | 3 | 12 | 16 | 27 | 44 | 29 | 18 | 33 | 31 | 41 | 20 | 19 | 33 | 36 |
Golgi | 37 | 60 | 5 | 19 | 16 | 16 | 13 | – | 3 | 8 | 3 | 4 | 12 | 20 | 38 | 93 | 87 | 61 | 90 | 105 | 32 | 16 | 52 | 48 |
Mitochondria | 69 | 45 | 2 | 36 | 20 | 88 | 105 | – | 175 | 154 | 245 | 214 | 4 | 5 | 10 | 31 | 22 | 21 | 23 | 42 | 42 | 33 | 56 | 55 |
Nucleus | 0 | 0 | 1 | 4 | 0 | 0 | 3 | – | 3 | 3 | 2 | 7 | 5 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 3 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 2 | 4 | 2 | – | 2 | 4 | 2 | 4 | 3 | 7 | 3 | 8 | 5 | 4 | 2 | 2 | 0 | 0 | 3 | 2 |
Vac/Vac Membrane | 9 | 18 | 12 | 8 | 17 | 23 | 13 | – | 20 | 17 | 11 | 25 | 10 | 13 | 28 | 71 | 55 | 56 | 13 | 41 | 19 | 20 | 28 | 39 |
Unique Cell Count | 152 | 224 | 68 | 104 | 111 | 202 | 252 | 252 | 244 | 277 | 327 | 166 | 173 | 243 | 270 | 224 | 215 | 193 | 332 | 136 | 124 | 239 | 282 | |
Labelled Cell Count | 206 | 284 | 74 | 130 | 132 | 242 | 296 | 308 | 299 | 356 | 394 | 185 | 202 | 274 | 338 | 278 | 274 | 193 | 332 | 136 | 124 | 239 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 9.3 | 9.1 | 7.7 | 8.2 | 7.2 | 7.1 | – | 6.1 | 6.2 | 5.5 | 5.9 | 8.8 | 8.4 | 8.6 | 11.1 | 11.3 | 10.7 | 8.2 | 8.5 | 9.0 |
Std Deviation (1e-4) | 0.9 | 1.5 | 1.4 | 1.4 | 1.4 | 1.9 | 1.6 | – | 1.5 | 1.4 | 1.2 | 1.3 | 1.3 | 1.0 | 1.3 | 1.9 | 2.4 | 2.4 | 1.2 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.23 | -0.15 | -0.34 | -0.36 | – | -0.58 | -0.54 | -0.72 | -0.63 | -0.05 | -0.11 | -0.07 | 0.29 | 0.31 | 0.24 | -0.15 | -0.1 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | -0.3 | -0.5 | -1.0 | – | 0 | -0.7 | 0 | 0 | 0 | 0.2 | 0 | 0.1 | 0.1 | -0.2 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.2 | -1.9 | -1.4 | -0.7 | – | -3.1 | -2.5 | -4.4 | -2.3 | 2.1 | 1.3 | 2.1 | -2.4 | -2.2 | -1.6 |
Endoplasmic Reticulum | 1.3 | 0.2 | -0.2 | 0.4 | – | 0 | -0.6 | -0.9 | -0.8 | 1.6 | 0.9 | -1.0 | 0 | 0 | -1.3 |
Endosome | -1.5 | 0.8 | -2.6 | -1.7 | – | -2.6 | 0 | 0 | 0 | -1.7 | -0.4 | 0.1 | -1.6 | -2.3 | -0.5 |
Golgi | 2.0 | 1.4 | 0.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.0 | 1.7 | 4.4 | 4.9 | 3.6 |
Mitochondria | 4.9 | 3.0 | 6.1 | 6.0 | – | 9.8 | 8.8 | 14.0 | 9.4 | 0 | 0 | 0 | 2.1 | 1.8 | 1.8 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.0 | -0.4 | -1.3 | -3.4 | – | -2.4 | -2.7 | 0 | -2.6 | -2.8 | -2.3 | -1.3 | 1.5 | 1.2 | 1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2704 | 2.4442 | 1.9809 | 1.8164 | 1.7184 | 2.1043 | 2.2205 | 3.3872 | 2.8536 | 2.7793 | 2.4549 | 3.0999 | 2.667 | 3.5768 | 3.1119 | 2.9386 | 2.7801 | 3.0564 |
Actin | 0.0306 | 0.0117 | 0.0175 | 0.0671 | 0.0024 | 0.0064 | 0.0149 | 0.0021 | 0.0126 | 0.0105 | 0.0034 | 0.0044 | 0.0092 | 0.0043 | 0.0019 | 0.002 | 0.0015 | 0.002 |
Bud | 0.0009 | 0.0013 | 0.0026 | 0.0029 | 0.0008 | 0.0007 | 0.0011 | 0.0029 | 0.0106 | 0.0049 | 0.0014 | 0.0015 | 0.0009 | 0.0016 | 0.0013 | 0.0035 | 0.0005 | 0.0005 |
Bud Neck | 0.0036 | 0.0012 | 0.0011 | 0.0035 | 0.0005 | 0.0045 | 0.0007 | 0.0006 | 0.0019 | 0.0043 | 0.0005 | 0.0013 | 0.0009 | 0.0006 | 0.0005 | 0.0004 | 0.0005 | 0.0011 |
Bud Periphery | 0.0014 | 0.0012 | 0.002 | 0.003 | 0.0004 | 0.0008 | 0.0009 | 0.0012 | 0.0062 | 0.0023 | 0.0006 | 0.001 | 0.0006 | 0.0005 | 0.0006 | 0.0058 | 0.0002 | 0.0003 |
Bud Site | 0.0101 | 0.0092 | 0.0167 | 0.0086 | 0.0012 | 0.0012 | 0.0048 | 0.0107 | 0.0077 | 0.0025 | 0.0008 | 0.0005 | 0.005 | 0.0104 | 0.0017 | 0.0011 | 0.0005 | 0.0005 |
Cell Periphery | 0.0019 | 0.0017 | 0.0009 | 0.0009 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0935 | 0.184 | 0.1016 | 0.0303 | 0.0897 | 0.1111 | 0.1291 | 0.2565 | 0.1078 | 0.0904 | 0.038 | 0.1104 | 0.0853 | 0.152 | 0.1068 | 0.0743 | 0.073 | 0.0949 |
Cytoplasmic Foci | 0.1218 | 0.1569 | 0.108 | 0.1286 | 0.1677 | 0.1509 | 0.1376 | 0.1522 | 0.1617 | 0.1326 | 0.1201 | 0.1641 | 0.138 | 0.1698 | 0.1531 | 0.1032 | 0.1246 | 0.1444 |
Eisosomes | 0.0009 | 0.0004 | 0.0009 | 0.0006 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0136 | 0.006 | 0.0101 | 0.002 | 0.0018 | 0.0044 | 0.0109 | 0.0045 | 0.0063 | 0.0033 | 0.0029 | 0.003 | 0.0057 | 0.0038 | 0.0031 | 0.0024 | 0.0025 | 0.0055 |
Endosome | 0.4314 | 0.3682 | 0.4745 | 0.4648 | 0.5141 | 0.4275 | 0.4381 | 0.4132 | 0.4389 | 0.5458 | 0.6133 | 0.4582 | 0.5361 | 0.4775 | 0.5272 | 0.6392 | 0.6518 | 0.4894 |
Golgi | 0.1805 | 0.187 | 0.1804 | 0.1624 | 0.1782 | 0.2131 | 0.1471 | 0.1147 | 0.1711 | 0.1319 | 0.1442 | 0.1883 | 0.1641 | 0.1455 | 0.1608 | 0.1166 | 0.1077 | 0.2096 |
Lipid Particles | 0.0197 | 0.005 | 0.0069 | 0.0088 | 0.0105 | 0.0068 | 0.0115 | 0.001 | 0.0045 | 0.0051 | 0.0036 | 0.0017 | 0.0041 | 0.001 | 0.0012 | 0.0006 | 0.0037 | 0.0014 |
Mitochondria | 0.016 | 0.0301 | 0.0357 | 0.0649 | 0.0097 | 0.0452 | 0.0403 | 0.0115 | 0.0248 | 0.0193 | 0.0253 | 0.0355 | 0.0212 | 0.0119 | 0.0224 | 0.023 | 0.0082 | 0.0329 |
None | 0.0203 | 0.0166 | 0.0091 | 0.0005 | 0.0011 | 0.0009 | 0.0119 | 0.0093 | 0.0158 | 0.0154 | 0.0152 | 0.0061 | 0.0109 | 0.0023 | 0.0009 | 0.0007 | 0.0005 | 0.0016 |
Nuclear Periphery | 0.0103 | 0.001 | 0.003 | 0.0005 | 0.0004 | 0.0006 | 0.0084 | 0.0012 | 0.0016 | 0.0013 | 0.0012 | 0.0008 | 0.0008 | 0.0006 | 0.0006 | 0.0007 | 0.001 | 0.0008 |
Nucleolus | 0.001 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.002 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0022 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0026 | 0.0006 | 0.0008 | 0.0005 | 0.0003 | 0.0012 | 0.0023 | 0.0007 | 0.0013 | 0.0015 | 0.0011 | 0.0004 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.0004 | 0.0005 |
Peroxisomes | 0.015 | 0.0024 | 0.009 | 0.0366 | 0.0052 | 0.0067 | 0.0106 | 0.0018 | 0.0074 | 0.0066 | 0.0085 | 0.009 | 0.0035 | 0.0046 | 0.0048 | 0.0014 | 0.0034 | 0.0044 |
Punctate Nuclear | 0.0095 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0005 | 0.003 | 0.0004 | 0.0026 | 0.002 | 0.0017 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0003 |
Vacuole | 0.0106 | 0.0109 | 0.0123 | 0.0083 | 0.0097 | 0.0099 | 0.0199 | 0.0113 | 0.0108 | 0.0131 | 0.0079 | 0.0074 | 0.0083 | 0.0083 | 0.0073 | 0.0159 | 0.0108 | 0.0056 |
Vacuole Periphery | 0.0047 | 0.004 | 0.0062 | 0.0046 | 0.0054 | 0.0049 | 0.006 | 0.0037 | 0.0054 | 0.0065 | 0.0079 | 0.0054 | 0.0042 | 0.0046 | 0.0051 | 0.0085 | 0.0087 | 0.0044 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.9436 | 12.7782 | 10.2113 | 16.3243 | 16.0383 | 12.0884 | 17.5165 | 14.4217 | 16.7192 | 18.765 |
Translational Efficiency | 2.1598 | 2.1549 | 2.6836 | 1.6947 | 1.5758 | 2.684 | 1.5362 | 1.9067 | 1.6498 | 1.6158 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1799 | 1362 | 933 | 1609 | 896 | 1427 | 118 | 181 | 2695 | 2789 | 1051 | 1790 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 820.70 | 806.59 | 1213.47 | 1134.58 | 761.30 | 906.94 | 1234.49 | 1179.13 | 800.95 | 857.93 | 1215.83 | 1139.08 |
Standard Deviation | 102.70 | 132.10 | 159.77 | 140.41 | 228.15 | 130.60 | 138.18 | 142.97 | 158.52 | 140.59 | 157.63 | 141.31 |
Intensity Change Log 2 | — | -0.025019 | 0.564212 | 0.467231 | — | 0.252542 | 0.697378 | 0.631186 | — | 0.115222 | 0.629830 | 0.548458 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000774 | 0.000619 | 0.001402 | 0.004498 | 0.000429 | 0.002004 | 0.002744 | 0.004605 | 0.000660 | 0.001328 | 0.001552 | 0.004509 |
Bud Neck | 0.001185 | 0.001207 | 0.001428 | 0.003598 | 0.010933 | 0.001505 | 0.002319 | 0.005182 | 0.004426 | 0.001359 | 0.001528 | 0.003759 |
Bud Site | 0.012069 | 0.014121 | 0.017654 | 0.067455 | 0.018095 | 0.018542 | 0.033079 | 0.056435 | 0.014072 | 0.016383 | 0.019386 | 0.066341 |
Cell Periphery | 0.000169 | 0.000164 | 0.000100 | 0.000126 | 0.001745 | 0.000313 | 0.000134 | 0.000105 | 0.000693 | 0.000240 | 0.000104 | 0.000124 |
Cytoplasm | 0.020160 | 0.027049 | 0.021432 | 0.366922* | 0.060373 | 0.026644 | 0.051363 | 0.207988* | 0.033530 | 0.026842 | 0.024793 | 0.350851* |
Cytoplasmic Foci | 0.250009* | 0.311552* | 0.215902* | 0.274431* | 0.266021* | 0.305060* | 0.326622* | 0.319800* | 0.255332* | 0.308231* | 0.228333* | 0.279019* |
Eisosomes | 0.000087 | 0.000065 | 0.000019 | 0.000029 | 0.000889 | 0.000145 | 0.000045 | 0.000040 | 0.000353 | 0.000106 | 0.000022 | 0.000030 |
Endoplasmic Reticulum | 0.001325 | 0.003233 | 0.004403 | 0.000491 | 0.005919 | 0.001616 | 0.006776 | 0.000660 | 0.002852 | 0.002406 | 0.004669 | 0.000508 |
Endosome | 0.099081 | 0.151347 | 0.164600 | 0.065860 | 0.125513 | 0.145020 | 0.218586* | 0.104458 | 0.107869 | 0.148110 | 0.170661 | 0.069763 |
Golgi | 0.471196* | 0.362580* | 0.259809* | 0.051673 | 0.274267* | 0.388663* | 0.136556* | 0.012767 | 0.405723* | 0.375925* | 0.245970* | 0.047739 |
Lipid Particles | 0.003893 | 0.007845 | 0.004684 | 0.003838 | 0.044067 | 0.002313 | 0.021512 | 0.007052 | 0.017249 | 0.005015 | 0.006573 | 0.004163 |
Mitochondria | 0.085339 | 0.034746 | 0.004057 | 0.001127 | 0.048366 | 0.043999 | 0.000783 | 0.003007 | 0.073047 | 0.039480 | 0.003690 | 0.001317 |
Mitotic Spindle | 0.005312 | 0.007456 | 0.084261 | 0.029854 | 0.002071 | 0.003162 | 0.046645 | 0.042272 | 0.004235 | 0.005259 | 0.080038 | 0.031110 |
None | 0.000309 | 0.000389 | 0.000072 | 0.000050 | 0.040226 | 0.000057 | 0.000037 | 0.000097 | 0.013580 | 0.000219 | 0.000068 | 0.000055 |
Nuclear Periphery | 0.000060 | 0.000342 | 0.001391 | 0.000122 | 0.000648 | 0.000035 | 0.000722 | 0.000131 | 0.000255 | 0.000185 | 0.001316 | 0.000123 |
Nuclear Periphery Foci | 0.000916 | 0.003188 | 0.010157 | 0.000772 | 0.009826 | 0.000232 | 0.010915 | 0.001539 | 0.003878 | 0.001676 | 0.010242 | 0.000849 |
Nucleolus | 0.000373 | 0.001775 | 0.000188 | 0.000109 | 0.006160 | 0.000037 | 0.000255 | 0.000164 | 0.002297 | 0.000886 | 0.000196 | 0.000114 |
Nucleus | 0.000272 | 0.001813 | 0.000785 | 0.002121 | 0.010790 | 0.000178 | 0.001611 | 0.002395 | 0.003769 | 0.000976 | 0.000878 | 0.002149 |
Peroxisomes | 0.002827 | 0.002852 | 0.001654 | 0.014014 | 0.005651 | 0.003207 | 0.008502 | 0.021908 | 0.003766 | 0.003033 | 0.002423 | 0.014812 |
Vacuole | 0.012183 | 0.027934 | 0.147284 | 0.104969 | 0.028154 | 0.022416 | 0.113016 | 0.206002* | 0.017493 | 0.025111 | 0.143437 | 0.115185 |
Vacuole Periphery | 0.032463 | 0.039722 | 0.058717 | 0.007940 | 0.039857 | 0.034851 | 0.017779 | 0.003392 | 0.034921 | 0.037230 | 0.054121 | 0.007480 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 1.05 | -1.77 | -5.03 | -5.35 | -3.22 | -3.92 | -3.76 | -3.02 | -1.69 | -1.14 | -2.96 | -2.58 | -5.72 | -4.43 | -3.38 |
Bud Neck | -0.09 | -1.99 | -11.96 | -10.19 | -10.45 | 5.99 | 5.33 | 3.57 | -5.90 | -4.06 | 5.55 | 5.09 | 0.92 | -11.48 | -11.14 |
Bud Site | -1.55 | -3.28 | -18.76 | -17.62 | -14.58 | -0.12 | -2.52 | -5.68 | -5.89 | -2.57 | -1.73 | -3.09 | -18.58 | -17.94 | -14.59 |
Cell Periphery | 0.13 | 2.48 | 1.89 | 2.41 | -1.43 | 4.87 | 6.19 | 6.33 | 1.53 | 1.45 | 3.96 | 6.57 | 6.45 | 1.74 | -0.98 |
Cytoplasm | -2.65 | -1.02 | -46.63 | -44.98 | -45.52* | 7.37 | -0.17 | -9.11 | -11.05 | -7.49 | 2.98 | 2.05 | -45.18 | -46.66 | -44.88 |
Cytoplasmic Foci | -10.57 | 5.67 | -0.42 | 8.31 | -5.14 | -4.87 | -2.32 | -0.41 | 1.83 | 1.59 | -11.67 | 4.99 | 0.16 | 8.89 | -4.05 |
Eisosomes | 2.61 | 11.64 | 10.26 | 6.01 | -2.91 | 3.07 | 3.49 | 3.51 | 5.81 | 0.56 | 3.04 | 4.09 | 4.03 | 8.86 | -2.22 |
Endoplasmic Reticulum | -3.51 | -4.55 | 5.70 | 5.28 | 5.92 | 4.99 | -0.26 | 6.45 | 3.58 | 2.05 | 1.04 | -2.54 | 8.10 | 6.62 | 6.18 |
Endosome | -11.56 | -12.44 | 12.26 | 21.22 | 20.77 | -3.59 | -4.82 | 4.39 | 6.80 | 6.67 | -11.93 | -12.18 | 15.02 | 26.10 | 21.60 |
Golgi | 12.15 | 21.11 | 65.33* | 45.00* | 24.79* | -10.78 | 8.12 | 29.52 | 54.59* | 7.75 | 4.11 | 17.64 | 63.45* | 64.27* | 25.49* |
Lipid Particles | -3.04 | -1.12 | -0.58 | 2.92 | 0.80 | 9.51 | 3.17 | 8.22 | -3.76 | 2.35 | 7.32 | 5.98 | 8.09 | 0.77 | 2.26 |
Mitochondria | 12.28 | 23.02 | 24.47 | 14.90 | 3.90 | 0.96 | 15.34 | 13.57 | 14.10 | -0.93 | 10.87 | 26.32 | 28.22 | 21.97 | 3.62 |
Mitotic Spindle | -1.58 | -13.31 | -8.96 | -7.95 | 8.11 | -1.72 | -3.77 | -3.81 | -3.70 | 0.66 | -1.28 | -13.97 | -10.10 | -9.71 | 7.97 |
None | -0.65 | 2.53 | 2.70 | 4.39 | 1.27 | 8.01 | 8.01 | 8.00 | -1.41 | -0.50 | 7.83 | 7.92 | 7.92 | 4.14 | 0.84 |
Nuclear Periphery | -2.88 | -8.52 | -3.00 | 1.97 | 7.95 | 3.31 | -0.34 | 2.52 | -6.26 | 1.98 | 0.89 | -6.94 | 1.61 | 0.70 | 8.18 |
Nuclear Periphery Foci | -2.70 | -6.57 | -0.06 | 3.03 | 6.83 | 5.67 | -0.60 | 4.67 | -5.27 | 2.33 | 3.06 | -4.49 | 4.51 | 1.72 | 7.20 |
Nucleolus | -1.82 | 0.57 | 0.83 | 2.36 | 1.08 | 5.45 | 5.26 | 5.34 | -4.16 | 0.82 | 2.76 | 4.90 | 5.12 | 2.21 | 1.21 |
Nucleus | -2.17 | -2.83 | -6.87 | -1.75 | -4.70 | 7.33 | 3.90 | 4.80 | -5.79 | -0.13 | 4.65 | 4.66 | 0.85 | -4.36 | -4.41 |
Peroxisomes | -0.10 | 2.65 | -11.59 | -11.35 | -12.87 | 3.15 | -1.61 | -4.23 | -5.16 | -3.24 | 1.90 | 3.12 | -11.32 | -12.49 | -12.93 |
Vacuole | -8.89 | -22.72 | -28.95 | -23.78 | 2.71 | 2.13 | -5.75 | -12.31 | -12.65 | -5.28 | -5.63 | -22.65 | -29.65 | -27.56 | 0.59 |
Vacuole Periphery | -2.83 | -7.33 | 14.85 | 15.38 | 16.01 | 1.39 | 4.20 | 13.10 | 17.11 | 3.07 | -1.27 | -5.89 | 18.51 | 20.74 | 16.13 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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