Standard name | |
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Human Ortholog | |
Description | Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 14 | 32 | 7 | 12 | 10 | 17 | 17 | – | 10 | 19 | 9 | 10 | 17 | 19 | 6 | 29 | 24 | 20 | 7 | 22 | 5 | 6 | 9 | 9 |
Bud | 2 | 2 | 1 | 2 | 1 | 0 | 3 | – | 12 | 9 | 19 | 9 | 0 | 1 | 0 | 1 | 3 | 5 | 2 | 9 | 0 | 2 | 2 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 1 | 3 | 1 | 2 | 3 | – | 0 | 3 | 1 | 4 | 0 | 1 | 2 | 4 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 39 | 57 | 24 | 15 | 25 | 53 | 77 | – | 45 | 50 | 35 | 72 | 83 | 77 | 121 | 58 | 49 | 54 | 11 | 36 | 3 | 14 | 31 | 47 |
Endoplasmic Reticulum | 23 | 56 | 8 | 20 | 14 | 22 | 34 | – | 15 | 23 | 23 | 28 | 34 | 28 | 19 | 12 | 11 | 15 | 7 | 21 | 3 | 3 | 13 | 22 |
Endosome | 13 | 11 | 12 | 10 | 25 | 14 | 26 | – | 18 | 8 | 3 | 12 | 16 | 27 | 44 | 29 | 18 | 33 | 31 | 41 | 20 | 19 | 33 | 36 |
Golgi | 37 | 60 | 5 | 19 | 16 | 16 | 13 | – | 3 | 8 | 3 | 4 | 12 | 20 | 38 | 93 | 87 | 61 | 90 | 105 | 32 | 16 | 52 | 48 |
Mitochondria | 69 | 45 | 2 | 36 | 20 | 88 | 105 | – | 175 | 154 | 245 | 214 | 4 | 5 | 10 | 31 | 22 | 21 | 23 | 42 | 42 | 33 | 56 | 55 |
Nucleus | 0 | 0 | 1 | 4 | 0 | 0 | 3 | – | 3 | 3 | 2 | 7 | 5 | 4 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 3 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 1 | 2 | 4 | 2 | – | 2 | 4 | 2 | 4 | 3 | 7 | 3 | 8 | 5 | 4 | 2 | 2 | 0 | 0 | 3 | 2 |
Vac/Vac Membrane | 9 | 18 | 12 | 8 | 17 | 23 | 13 | – | 20 | 17 | 11 | 25 | 10 | 13 | 28 | 71 | 55 | 56 | 13 | 41 | 19 | 20 | 28 | 39 |
Unique Cell Count | 152 | 224 | 68 | 104 | 111 | 202 | 252 | 252 | 244 | 277 | 327 | 166 | 173 | 243 | 270 | 224 | 215 | 193 | 332 | 136 | 124 | 239 | 282 | |
Labelled Cell Count | 206 | 284 | 74 | 130 | 132 | 242 | 296 | 308 | 299 | 356 | 394 | 185 | 202 | 274 | 338 | 278 | 274 | 193 | 332 | 136 | 124 | 239 | 282 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.4 | 9.3 | 9.1 | 7.7 | 8.2 | 7.2 | 7.1 | – | 6.1 | 6.2 | 5.5 | 5.9 | 8.8 | 8.4 | 8.6 | 11.1 | 11.3 | 10.7 | 8.2 | 8.5 | 9.0 |
Std Deviation (1e-4) | 0.9 | 1.5 | 1.4 | 1.4 | 1.4 | 1.9 | 1.6 | – | 1.5 | 1.4 | 1.2 | 1.3 | 1.3 | 1.0 | 1.3 | 1.9 | 2.4 | 2.4 | 1.2 | 1.3 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.23 | -0.15 | -0.34 | -0.36 | – | -0.58 | -0.54 | -0.72 | -0.63 | -0.05 | -0.11 | -0.07 | 0.29 | 0.31 | 0.24 | -0.15 | -0.1 | -0.02 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.3 | -0.3 | -0.5 | -1.0 | – | 0 | -0.7 | 0 | 0 | 0 | 0.2 | 0 | 0.1 | 0.1 | -0.2 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -3.2 | -1.9 | -1.4 | -0.7 | – | -3.1 | -2.5 | -4.4 | -2.3 | 2.1 | 1.3 | 2.1 | -2.4 | -2.2 | -1.6 |
Endoplasmic Reticulum | 1.3 | 0.2 | -0.2 | 0.4 | – | 0 | -0.6 | -0.9 | -0.8 | 1.6 | 0.9 | -1.0 | 0 | 0 | -1.3 |
Endosome | -1.5 | 0.8 | -2.6 | -1.7 | – | -2.6 | 0 | 0 | 0 | -1.7 | -0.4 | 0.1 | -1.6 | -2.3 | -0.5 |
Golgi | 2.0 | 1.4 | 0.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1.0 | 1.7 | 4.4 | 4.9 | 3.6 |
Mitochondria | 4.9 | 3.0 | 6.1 | 6.0 | – | 9.8 | 8.8 | 14.0 | 9.4 | 0 | 0 | 0 | 2.1 | 1.8 | 1.8 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.0 | -0.4 | -1.3 | -3.4 | – | -2.4 | -2.7 | 0 | -2.6 | -2.8 | -2.3 | -1.3 | 1.5 | 1.2 | 1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2704 | 2.4442 | 1.9809 | 1.8164 | 1.7184 | 2.1043 | 2.2205 | 3.3872 | 2.8536 | 2.7793 | 2.4549 | 3.0999 | 2.667 | 3.5768 | 3.1119 | 2.9386 | 2.7801 | 3.0564 |
Actin | 0.0306 | 0.0117 | 0.0175 | 0.0671 | 0.0024 | 0.0064 | 0.0149 | 0.0021 | 0.0126 | 0.0105 | 0.0034 | 0.0044 | 0.0092 | 0.0043 | 0.0019 | 0.002 | 0.0015 | 0.002 |
Bud | 0.0009 | 0.0013 | 0.0026 | 0.0029 | 0.0008 | 0.0007 | 0.0011 | 0.0029 | 0.0106 | 0.0049 | 0.0014 | 0.0015 | 0.0009 | 0.0016 | 0.0013 | 0.0035 | 0.0005 | 0.0005 |
Bud Neck | 0.0036 | 0.0012 | 0.0011 | 0.0035 | 0.0005 | 0.0045 | 0.0007 | 0.0006 | 0.0019 | 0.0043 | 0.0005 | 0.0013 | 0.0009 | 0.0006 | 0.0005 | 0.0004 | 0.0005 | 0.0011 |
Bud Periphery | 0.0014 | 0.0012 | 0.002 | 0.003 | 0.0004 | 0.0008 | 0.0009 | 0.0012 | 0.0062 | 0.0023 | 0.0006 | 0.001 | 0.0006 | 0.0005 | 0.0006 | 0.0058 | 0.0002 | 0.0003 |
Bud Site | 0.0101 | 0.0092 | 0.0167 | 0.0086 | 0.0012 | 0.0012 | 0.0048 | 0.0107 | 0.0077 | 0.0025 | 0.0008 | 0.0005 | 0.005 | 0.0104 | 0.0017 | 0.0011 | 0.0005 | 0.0005 |
Cell Periphery | 0.0019 | 0.0017 | 0.0009 | 0.0009 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 |
Cytoplasm | 0.0935 | 0.184 | 0.1016 | 0.0303 | 0.0897 | 0.1111 | 0.1291 | 0.2565 | 0.1078 | 0.0904 | 0.038 | 0.1104 | 0.0853 | 0.152 | 0.1068 | 0.0743 | 0.073 | 0.0949 |
Cytoplasmic Foci | 0.1218 | 0.1569 | 0.108 | 0.1286 | 0.1677 | 0.1509 | 0.1376 | 0.1522 | 0.1617 | 0.1326 | 0.1201 | 0.1641 | 0.138 | 0.1698 | 0.1531 | 0.1032 | 0.1246 | 0.1444 |
Eisosomes | 0.0009 | 0.0004 | 0.0009 | 0.0006 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0136 | 0.006 | 0.0101 | 0.002 | 0.0018 | 0.0044 | 0.0109 | 0.0045 | 0.0063 | 0.0033 | 0.0029 | 0.003 | 0.0057 | 0.0038 | 0.0031 | 0.0024 | 0.0025 | 0.0055 |
Endosome | 0.4314 | 0.3682 | 0.4745 | 0.4648 | 0.5141 | 0.4275 | 0.4381 | 0.4132 | 0.4389 | 0.5458 | 0.6133 | 0.4582 | 0.5361 | 0.4775 | 0.5272 | 0.6392 | 0.6518 | 0.4894 |
Golgi | 0.1805 | 0.187 | 0.1804 | 0.1624 | 0.1782 | 0.2131 | 0.1471 | 0.1147 | 0.1711 | 0.1319 | 0.1442 | 0.1883 | 0.1641 | 0.1455 | 0.1608 | 0.1166 | 0.1077 | 0.2096 |
Lipid Particles | 0.0197 | 0.005 | 0.0069 | 0.0088 | 0.0105 | 0.0068 | 0.0115 | 0.001 | 0.0045 | 0.0051 | 0.0036 | 0.0017 | 0.0041 | 0.001 | 0.0012 | 0.0006 | 0.0037 | 0.0014 |
Mitochondria | 0.016 | 0.0301 | 0.0357 | 0.0649 | 0.0097 | 0.0452 | 0.0403 | 0.0115 | 0.0248 | 0.0193 | 0.0253 | 0.0355 | 0.0212 | 0.0119 | 0.0224 | 0.023 | 0.0082 | 0.0329 |
None | 0.0203 | 0.0166 | 0.0091 | 0.0005 | 0.0011 | 0.0009 | 0.0119 | 0.0093 | 0.0158 | 0.0154 | 0.0152 | 0.0061 | 0.0109 | 0.0023 | 0.0009 | 0.0007 | 0.0005 | 0.0016 |
Nuclear Periphery | 0.0103 | 0.001 | 0.003 | 0.0005 | 0.0004 | 0.0006 | 0.0084 | 0.0012 | 0.0016 | 0.0013 | 0.0012 | 0.0008 | 0.0008 | 0.0006 | 0.0006 | 0.0007 | 0.001 | 0.0008 |
Nucleolus | 0.001 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.002 | 0.0003 | 0.0002 | 0.0003 | 0.0004 | 0.0022 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Nucleus | 0.0026 | 0.0006 | 0.0008 | 0.0005 | 0.0003 | 0.0012 | 0.0023 | 0.0007 | 0.0013 | 0.0015 | 0.0011 | 0.0004 | 0.0005 | 0.0005 | 0.0003 | 0.0004 | 0.0004 | 0.0005 |
Peroxisomes | 0.015 | 0.0024 | 0.009 | 0.0366 | 0.0052 | 0.0067 | 0.0106 | 0.0018 | 0.0074 | 0.0066 | 0.0085 | 0.009 | 0.0035 | 0.0046 | 0.0048 | 0.0014 | 0.0034 | 0.0044 |
Punctate Nuclear | 0.0095 | 0.0004 | 0.0005 | 0.0004 | 0.0005 | 0.0005 | 0.003 | 0.0004 | 0.0026 | 0.002 | 0.0017 | 0.0004 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0003 |
Vacuole | 0.0106 | 0.0109 | 0.0123 | 0.0083 | 0.0097 | 0.0099 | 0.0199 | 0.0113 | 0.0108 | 0.0131 | 0.0079 | 0.0074 | 0.0083 | 0.0083 | 0.0073 | 0.0159 | 0.0108 | 0.0056 |
Vacuole Periphery | 0.0047 | 0.004 | 0.0062 | 0.0046 | 0.0054 | 0.0049 | 0.006 | 0.0037 | 0.0054 | 0.0065 | 0.0079 | 0.0054 | 0.0042 | 0.0046 | 0.0051 | 0.0085 | 0.0087 | 0.0044 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 15.9436 | 12.7782 | 10.2113 | 16.3243 | 16.0383 | 12.0884 | 17.5165 | 14.4217 | 16.7192 | 18.765 |
Translational Efficiency | 2.1598 | 2.1549 | 2.6836 | 1.6947 | 1.5758 | 2.684 | 1.5362 | 1.9067 | 1.6498 | 1.6158 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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