Standard name
Human Ortholog
Description Sterol-binding protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; contains two StART-like domains that bind sterols and a GRAM domain; co-localizes to puncta in the cortical ER with Sip3p; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.05 0 0 0 0 0 0 0 0 – 0 0 0.07 0 0 – 0.06 0.05 0.22 0.18 0.13 0.18 0.25 0.34
Bud 0 0 0.05 0 0 0.09 0.08 0.1 0.11 – 0.11 0.09 0.07 0.05 0.06 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0.07 0.05 0.09 0.08 0.07
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 0 0 – 0 0 0.08 0.08 0.08 – 0.06 0.05 0.07 0.13 0.15 0.17 0.15 0.21
Cytoplasm 0.88 0.89 0.95 0.92 0.88 0.73 0.68 0.7 0.62 – 0.49 0.45 0.87 0.9 0.94 – 0.8 0.74 0.18 0.35 0.28 0.24 0.23 0.12
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0.06 0.07 0.1 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.07 0 0.11 0.05 0.24 0.34 0.27 0.47 – 0.53 0.61 0 0 0 – 0 0.05 0.26 0.17 0.27 0.2 0.18 0.14
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0 0.06 0 0.05 – 0.08 0 0 0 0 – 0.08 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 6 1 2 0 4 3 7 0 – 1 0 11 13 9 – 18 28 36 40 16 29 78 107
Bud 2 3 6 8 8 28 40 53 31 – 23 33 10 16 22 – 5 13 0 2 0 1 4 3
Bud Neck 0 0 4 2 6 4 5 12 2 – 2 3 0 1 1 – 10 5 5 15 6 14 25 21
Bud Site 0 1 0 1 2 4 3 2 3 – 2 6 0 0 0 – 0 2 – – – – – –
Cell Periphery 3 7 5 6 4 16 14 7 9 – 9 6 12 24 29 – 17 29 12 28 19 26 46 67
Cytoplasm 74 166 125 207 296 220 330 363 168 – 101 171 131 266 361 – 238 393 29 78 35 38 72 38
Endoplasmic Reticulum 0 1 0 0 1 1 2 1 0 – 1 0 1 1 3 – 17 38 16 3 1 2 2 5
Endosome 0 0 0 0 0 1 1 0 0 – 0 0 0 1 0 – 3 5 7 2 2 4 8 11
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 1 0 0 – 0 0 1 1 0 0 0 1
Mitochondria 6 14 3 24 16 71 165 141 128 – 109 229 1 5 11 – 6 24 43 38 33 31 56 44
Nucleus 0 1 0 0 1 0 0 3 1 – 1 2 0 0 0 – 0 4 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 1 1 0 0 – 0 0 0 1 0 – 0 1 1 0 0 0 0 0
Nucleolus 0 0 1 0 0 0 0 0 0 – 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 1 5 6 2 1 – 0 2 1 1 1 – 2 19 4 0 2 1 2 6
SpindlePole 0 1 2 0 20 11 27 21 14 – 16 12 1 4 1 – 25 54 0 1 0 0 2 1
Vac/Vac Membrane 0 0 0 1 1 1 0 0 0 – 0 0 0 0 0 – 1 9 2 6 2 2 3 5
Unique Cell Count 84 187 132 224 337 300 487 515 273 205 376 150 295 383 296 530 165 226 125 159 309 315
Labelled Cell Count 89 200 147 251 356 367 597 612 357 266 464 169 333 438 342 624 165 226 125 159 309 315


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.2 3.6 3.7 3.2 4.0 3.5 2.9 3.3 3.0 – 2.9 2.4 4.6 4.2 3.8 4.8 5.1 5.7 4.0 4.1 4.5
Std Deviation (1e-4) 0.5 0.7 2.7 1.2 2.6 2.1 1.1 1.9 1.9 – 1.3 0.6 1.1 0.8 1.0 1.0 1.2 1.6 0.7 0.8 1.2
Intensity Change (Log2) – – – -0.17 0.13 -0.07 -0.31 -0.15 -0.29 – -0.32 -0.62 0.32 0.2 0.05 0.38 0.47 0.63 0.12 0.15 0.3


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1015 3.1985 2.665 2.5036 2.345 2.6512 1.246 1.7407 1.5335 1.3163 1.3625 1.4483 -0.5562 0.4275 0.0173 0.1981 -0.4556 0.0057
Actin 0.2121 0.1594 0.1935 0.1766 0.2679 0.2336 0.3051 0.1816 0.238 0.2063 0.1323 0.3308 0.1503 0.2658 0.0831 0.2195 0.1854 0.2051
Bud 0.0032 0.0015 0.0028 0.0069 0.0022 0.0033 0.0033 0.0017 0.0037 0.0019 0.0016 0.0014 0.0008 0.0008 0.0013 0.0028 0.0055 0.0029
Bud Neck 0.0365 0.0286 0.0981 0.1089 0.148 0.1832 0.0173 0.0223 0.0935 0.1281 0.2288 0.1428 0.0149 0.0296 0.1092 0.0858 0.1269 0.1251
Bud Periphery 0.0162 0.0056 0.0162 0.0328 0.0159 0.0215 0.0135 0.0047 0.0083 0.0089 0.0054 0.01 0.0037 0.0022 0.0053 0.0153 0.0451 0.0189
Bud Site 0.1161 0.4023 0.1489 0.091 0.0295 0.0194 0.0626 0.3163 0.1767 0.0313 0.0413 0.0084 0.0736 0.2824 0.1788 0.0891 0.0474 0.0118
Cell Periphery 0.0884 0.069 0.1316 0.1586 0.1052 0.1015 0.025 0.0406 0.0568 0.0332 0.0247 0.0438 0.0502 0.0756 0.086 0.0707 0.1249 0.0901
Cytoplasm 0.2354 0.1505 0.2506 0.2123 0.2904 0.2482 0.1691 0.2782 0.2646 0.3979 0.3577 0.2839 0.2166 0.185 0.2211 0.223 0.2522 0.2579
Cytoplasmic Foci 0.0455 0.0159 0.0181 0.0308 0.0162 0.0368 0.0523 0.0279 0.0248 0.0427 0.045 0.0317 0.0523 0.0165 0.054 0.0618 0.0211 0.0245
Eisosomes 0.0205 0.0144 0.0158 0.0072 0.0219 0.0172 0.0128 0.0058 0.0059 0.0088 0.0015 0.0077 0.0151 0.0317 0.0172 0.012 0.0086 0.0089
Endoplasmic Reticulum 0.0073 0.0049 0.0032 0.0042 0.0044 0.0026 0.0105 0.0022 0.0037 0.0057 0.0042 0.0025 0.0078 0.0011 0.0022 0.018 0.007 0.0019
Endosome 0.0143 0.0022 0.0028 0.0042 0.0105 0.0022 0.0507 0.0012 0.0063 0.0036 0.0617 0.0075 0.0025 0.0016 0.0016 0.0062 0.0015 0.0065
Golgi 0.0101 0.0029 0.0082 0.0071 0.009 0.0059 0.0142 0.0026 0.0071 0.0075 0.0401 0.0097 0.0046 0.0026 0.0038 0.0073 0.0028 0.0142
Lipid Particles 0.0131 0.0042 0.0033 0.0048 0.0099 0.0058 0.0284 0.0018 0.0025 0.0083 0.0104 0.0025 0.0061 0.0009 0.0051 0.007 0.0016 0.004
Mitochondria 0.0178 0.001 0.0038 0.0265 0.0389 0.0166 0.0118 0.0011 0.0099 0.0016 0.0098 0.0019 0.0012 0.0002 0.0012 0.0848 0.005 0.005
None 0.1347 0.1305 0.0911 0.0897 0.0181 0.0905 0.1589 0.1037 0.0865 0.0969 0.0261 0.1013 0.3921 0.1007 0.1996 0.0528 0.1471 0.2137
Nuclear Periphery 0.0014 0.0004 0.0004 0.0004 0.0017 0.0001 0.0037 0.0002 0.004 0.0004 0.0003 0.0036 0.0004 0.0001 0.0004 0.0054 0.0007 0.0003
Nucleolus 0.0013 0.0006 0.0009 0.0026 0.0006 0.0005 0.0022 0.0004 0.0003 0.0008 0.0001 0.0001 0.0005 0.0002 0.0009 0.0016 0.0005 0.0006
Nucleus 0.0016 0.0007 0.0009 0.0019 0.0007 0.0006 0.0021 0.0004 0.0011 0.0008 0.0004 0.0005 0.0005 0.0003 0.0022 0.0018 0.0006 0.0007
Peroxisomes 0.0161 0.0027 0.0054 0.0279 0.0062 0.0075 0.0279 0.0052 0.0045 0.0082 0.0026 0.0078 0.0046 0.0014 0.0206 0.027 0.0134 0.0038
Punctate Nuclear 0.0039 0.0006 0.001 0.0023 0.0007 0.001 0.0029 0.0005 0.0006 0.0016 0.0004 0.0008 0.0008 0.0003 0.0051 0.0034 0.0004 0.0022
Vacuole 0.0037 0.002 0.0032 0.003 0.0015 0.0016 0.022 0.0016 0.001 0.0053 0.004 0.001 0.0012 0.0009 0.0011 0.0031 0.0023 0.0016
Vacuole Periphery 0.0006 0.0001 0.0002 0.0004 0.0006 0.0003 0.0037 0.0001 0.0003 0.0003 0.0017 0.0003 0.0001 0 0.0001 0.0016 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.0707 25.819 20.5152 31.2411 21.3747 24.5236 35.8085 36.6423 30.3997 37.0836
Translational Efficiency 0.5739 0.5409 0.8262 0.4233 0.4397 0.5377 0.5492 0.4383 0.5429 0.4154

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1206 1518 387 722 1201 181 1511 169 2407 1699 1898 891

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 553.14 753.07 699.81 860.85 637.73 760.07 836.79 881.14 595.35 753.82 808.86 864.70
Standard Deviation 70.82 94.22 144.80 113.07 83.05 92.32 98.65 86.55 88.00 94.04 122.75 108.83
Intensity Change Log 2 — 0.445139 0.339319 0.638117 — 0.253187 0.391920 0.466426 — 0.345530 0.367726 0.548720

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002513 0.033192 0.043293 0.104068* 0.016460 0.003655 0.019001 0.247412* 0.009472 0.030045 0.023954 0.131257*
Bud Neck 0.048262 0.048756 0.008424 0.020506 0.034392 0.044047 0.009559 0.045991 0.041341 0.048254 0.009328 0.025340
Bud Site 0.010411 0.039921 0.059829 0.092397 0.017422 0.006789 0.021460 0.146876* 0.013909 0.036391 0.029283 0.102731*
Cell Periphery 0.004683 0.004567 0.005962 0.015397 0.003248 0.001557 0.006597 0.050449 0.003967 0.004247 0.006467 0.022045
Cytoplasm 0.474504* 0.379281* 0.255357* 0.279169* 0.399281* 0.337470* 0.437322* 0.164404* 0.436971* 0.374827* 0.400219* 0.257401*
Cytoplasmic Foci 0.175213 0.224385* 0.141450 0.072365 0.257303* 0.297033* 0.093390 0.057889 0.216173* 0.232124* 0.103189 0.069619
Eisosomes 0.009218 0.019163 0.001154 0.017466 0.012959 0.007308 0.004639 0.043124 0.011085 0.017900 0.003928 0.022333
Endoplasmic Reticulum 0.002091 0.002908 0.033586 0.007570 0.001680 0.001308 0.004466 0.012913 0.001886 0.002738 0.010404 0.008584
Endosome 0.004919 0.013047 0.019812 0.006525 0.008492 0.024071 0.005249 0.004481 0.006702 0.014222 0.008218 0.006138
Golgi 0.006553 0.023706 0.005510 0.004523 0.014850 0.010280 0.002157 0.001636 0.010693 0.022276 0.002840 0.003975
Lipid Particles 0.033229 0.016504 0.024288 0.007406 0.031601 0.022780 0.019470 0.007041 0.032417 0.017173 0.020452 0.007337
Mitochondria 0.008928 0.012982 0.004577 0.002677 0.009369 0.008810 0.000825 0.000178 0.009148 0.012537 0.001590 0.002203
Mitotic Spindle 0.000089 0.003383 0.003885 0.010826 0.002011 0.000206 0.001448 0.004612 0.001048 0.003045 0.001945 0.009647
None 0.008176 0.002092 0.011861 0.000430 0.004164 0.000797 0.007355 0.000142 0.006174 0.001954 0.008274 0.000376
Nuclear Periphery 0.000864 0.000155 0.000922 0.000351 0.000228 0.000443 0.000677 0.000136 0.000546 0.000186 0.000727 0.000310
Nuclear Periphery Foci 0.000470 0.000499 0.018018 0.001834 0.000409 0.000420 0.001355 0.001448 0.000439 0.000490 0.004753 0.001761
Nucleolus 0.000797 0.000296 0.004970 0.000107 0.001180 0.000958 0.000910 0.000039 0.000988 0.000366 0.001738 0.000094
Nucleus 0.170768* 0.082000 0.223867* 0.158639* 0.119469 0.130806* 0.294504* 0.071495 0.145172* 0.087200 0.280101* 0.142110*
Peroxisomes 0.020241 0.056402 0.029957 0.018840 0.042621 0.024645 0.009355 0.018975 0.031408 0.053019 0.013556 0.018866
Vacuole 0.017425 0.035466 0.102215 0.177438 0.022171 0.069527 0.060034 0.120673 0.019793 0.039094 0.068635 0.166671
Vacuole Periphery 0.000649 0.001295 0.001062 0.001463 0.000691 0.007090 0.000228 0.000087 0.000670 0.001912 0.000398 0.001202

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -13.32 -6.14 -11.42 -6.93 -4.97 5.52 -0.62 -9.16 -9.72 -9.06 -9.15 -5.39 -13.55 -10.57 -11.45
Bud Neck -0.15 10.83 8.10 10.97 -4.64 -1.08 10.71 -0.95 0.09 -3.91 -2.30 15.05 6.40 8.30 -6.33
Bud Site -11.06 -7.60 -11.21 -6.25 -2.87 5.98 -1.65 -7.91 -8.61 -7.61 -9.77 -6.54 -13.24 -9.01 -10.30
Cell Periphery 0.14 -0.26 -6.22 -6.67 -2.93 3.55 -3.28 -6.91 -7.18 -6.37 -0.56 -2.35 -9.46 -9.26 -7.41
Cytoplasm 10.37 17.14 19.05 10.41 -1.97 3.39 -3.11 13.40 7.34 15.33 8.02 6.02 20.60 12.95 14.51
Cytoplasmic Foci -7.02 3.99 16.89 23.73 6.95 -2.49 23.04 17.90 13.60 3.44 -2.48 21.83 27.33 26.87 6.80
Eisosomes -5.52 8.27 -2.57 1.91 -7.26 2.03 6.38 -3.82 -4.42 -5.09 -4.28 8.27 -3.83 -0.60 -7.58
Endoplasmic Reticulum -1.70 -9.62 -8.52 -6.98 7.77 0.86 -4.42 -5.95 -6.02 -4.36 -2.24 -9.68 -10.89 -8.77 1.43
Endosome -8.06 -5.37 -0.83 5.46 4.76 -3.11 3.45 1.78 3.62 0.32 -7.29 -1.45 1.16 6.61 2.02
Golgi -12.90 1.52 2.08 11.18 0.80 1.81 11.81 12.47 3.61 0.78 -9.20 11.43 6.24 12.25 -0.52
Lipid Particles 7.21 2.93 12.21 7.42 7.84 2.02 4.21 8.55 3.76 5.01 7.92 5.67 14.72 8.29 7.79
Mitochondria -2.74 3.51 4.26 6.18 1.11 0.17 7.73 8.35 2.33 4.83 -2.55 10.24 5.78 6.99 -0.31
Mitotic Spindle -3.02 -2.87 -3.55 -2.03 -1.89 2.00 0.55 -0.83 -1.46 -1.04 -1.83 -1.37 -3.28 -2.19 -2.89
None 4.70 -0.99 7.14 2.35 4.39 3.08 -1.80 4.09 1.48 5.38 4.35 -1.20 7.92 2.49 6.59
Nuclear Periphery 1.46 -0.28 0.89 -6.62 4.91 -1.84 -6.42 1.25 2.58 9.14 1.47 -0.82 0.68 -5.15 6.94
Nuclear Periphery Foci -0.20 -7.42 -6.36 -9.49 6.82 -0.11 -8.82 -3.98 -3.89 -0.53 -0.46 -8.32 -9.24 -10.00 5.60
Nucleolus 5.56 -4.46 7.72 6.80 5.18 0.38 0.56 2.72 2.34 3.10 2.90 -2.19 4.24 5.61 5.63
Nucleus 15.41 -4.78 0.40 -11.00 4.69 -0.94 -23.31 4.19 3.70 19.93 12.67 -21.47 -0.73 -9.18 16.17
Peroxisomes -11.32 -2.19 1.43 11.35 2.97 3.63 11.79 4.44 0.98 -1.77 -7.31 9.34 5.81 11.28 -1.48
Vacuole -10.00 -12.63 -23.04 -20.77 -8.86 -5.85 -15.68 -7.70 -4.28 -4.73 -10.69 -20.71 -23.86 -21.01 -15.43
Vacuole Periphery -2.50 -1.39 -0.97 0.19 -0.38 -1.58 4.70 6.66 1.74 2.08 -2.56 3.47 -0.68 1.42 -1.35
Standard name
Human Ortholog
Description Sterol-binding protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; contains two StART-like domains that bind sterols and a GRAM domain; co-localizes to puncta in the cortical ER with Sip3p; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes
Localization
Cell Percentages ER (6%), cytoplasm (4%), mixed (43%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ysp2

Ysp2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ysp2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available