Standard name | |
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Human Ortholog | |
Description | Evolutionarily conserved kinetochore protein; part of multiple protein complexes, including the SCF ubiquitin ligase complex, the CBF3 complex that binds centromeric DNA, and the RAVE complex that regulates assembly of the V-ATPase; protein abundance increases in response to DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 5 | 0 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 1 | 0 | 2 | 0 | 3 | – | 3 | 0 | 1 | 6 | 0 | 0 | 0 | 1 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 132 | 195 | 97 | 147 | 189 | 87 | 154 | – | 155 | 106 | 75 | 124 | 148 | 354 | 356 | 172 | 180 | 165 | 135 | 156 | 117 | 168 | 313 | 265 |
Endoplasmic Reticulum | 1 | 2 | 1 | 1 | 6 | 2 | 6 | – | 7 | 8 | 1 | 5 | 6 | 4 | 2 | 4 | 2 | 5 | 0 | 0 | 0 | 1 | 3 | 6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 2 | 0 |
Golgi | 1 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 5 | 0 | – | 11 | 9 | 6 | 28 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 2 |
Nucleus | 2 | 4 | 6 | 5 | 7 | 1 | 4 | – | 7 | 9 | 6 | 11 | 1 | 0 | 0 | 9 | 10 | 12 | 15 | 28 | 9 | 10 | 18 | 17 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Vac/Vac Membrane | 25 | 4 | 6 | 25 | 32 | 20 | 53 | – | 44 | 59 | 26 | 38 | 25 | 60 | 28 | 16 | 33 | 24 | 5 | 8 | 4 | 4 | 7 | 6 |
Unique Cell Count | 149 | 198 | 107 | 166 | 217 | 111 | 213 | 220 | 175 | 116 | 203 | 168 | 390 | 377 | 186 | 200 | 184 | 164 | 203 | 138 | 202 | 360 | 309 | |
Labelled Cell Count | 161 | 206 | 111 | 179 | 238 | 117 | 221 | 227 | 194 | 120 | 219 | 180 | 418 | 387 | 203 | 230 | 210 | 164 | 203 | 138 | 202 | 360 | 309 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.9 | 13.0 | 12.0 | 12.4 | 14.7 | 9.2 | 12.2 | – | 10.3 | 10.4 | 9.7 | 9.4 | 15.7 | 16.1 | 16.0 | 15.2 | 16.6 | 16.8 | 12.1 | 12.9 | 12.4 |
Std Deviation (1e-4) | 2.1 | 2.1 | 1.7 | 2.1 | 2.4 | 2.4 | 2.1 | – | 1.7 | 1.8 | 1.8 | 1.7 | 2.9 | 3.0 | 3.0 | 3.5 | 4.3 | 3.5 | 2.2 | 2.3 | 2.2 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.29 | -0.39 | 0.02 | – | -0.22 | -0.2 | -0.31 | -0.36 | 0.39 | 0.42 | 0.41 | 0.34 | 0.47 | 0.49 | 0.01 | 0.1 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.5 | -0.9 | -2.5 | -3.8 | – | -4.1 | -5.5 | -4.6 | -5.5 | -0.7 | 0 | 1.4 | 0.5 | -0.2 | -0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 4.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | -0.2 | -0.1 | -0.1 | 0 | 0 | 0 | -0.3 | -0.2 | 0.3 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.4 | 2.4 | 2.8 | 4.2 | – | 3.4 | 5.4 | 3.6 | 3.1 | 2.4 | 2.6 | 0.6 | 0.9 | 2.7 | 2.0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.0011 | 9.1773 | 8.2517 | 7.4055 | 6.337 | 7.8063 | 10.1454 | 13.2618 | 11.3967 | 11.9298 | 8.4014 | 11.056 | 10.2018 | 13.5134 | 11.1357 | 10.5866 | 10.0056 | 11.7687 |
Actin | 0.0331 | 0.0007 | 0.0121 | 0.001 | 0.0249 | 0.005 | 0.0087 | 0.0003 | 0.0313 | 0.0002 | 0.0527 | 0.0002 | 0.0192 | 0.0005 | 0.0025 | 0.0003 | 0.1552 | 0.0002 |
Bud | 0.0006 | 0.0002 | 0.0007 | 0.0001 | 0.0025 | 0.0003 | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0.0018 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0.0001 | 0.0012 | 0.0001 |
Bud Neck | 0.0054 | 0.0004 | 0.001 | 0.0005 | 0.0097 | 0.0018 | 0.0046 | 0.0003 | 0.0009 | 0.0005 | 0.0023 | 0.0012 | 0.0009 | 0.0005 | 0.0006 | 0.0007 | 0.0013 | 0.0011 |
Bud Periphery | 0.0007 | 0 | 0.001 | 0 | 0.0019 | 0.0003 | 0.0006 | 0 | 0.0007 | 0 | 0.0026 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0021 | 0 |
Bud Site | 0.0045 | 0.0025 | 0.004 | 0 | 0.0049 | 0.0002 | 0.0008 | 0.0002 | 0.0073 | 0.0001 | 0.0033 | 0 | 0.0006 | 0.0003 | 0.0009 | 0.0001 | 0.0023 | 0 |
Cell Periphery | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0004 | 0 |
Cytoplasm | 0.5625 | 0.8074 | 0.734 | 0.7245 | 0.6271 | 0.6618 | 0.7084 | 0.8429 | 0.7869 | 0.7592 | 0.6012 | 0.7527 | 0.769 | 0.8889 | 0.8163 | 0.8159 | 0.5928 | 0.7796 |
Cytoplasmic Foci | 0.0091 | 0.0014 | 0.0043 | 0.0014 | 0.0169 | 0.0031 | 0.0076 | 0.0005 | 0.0044 | 0.0006 | 0.0087 | 0.0006 | 0.0016 | 0.0007 | 0.0105 | 0.0012 | 0.0063 | 0.0011 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.001 | 0 |
Endoplasmic Reticulum | 0.0055 | 0.0016 | 0.0021 | 0.0006 | 0.0031 | 0.0043 | 0.0045 | 0.0006 | 0.0012 | 0.0009 | 0.0038 | 0.0016 | 0.0043 | 0.0009 | 0.0016 | 0.0008 | 0.0173 | 0.0013 |
Endosome | 0.0223 | 0.0007 | 0.0145 | 0.0005 | 0.0595 | 0.0236 | 0.0103 | 0.0003 | 0.0023 | 0.0006 | 0.0284 | 0.0004 | 0.0046 | 0.0002 | 0.0071 | 0.0004 | 0.0646 | 0.0008 |
Golgi | 0.0112 | 0.0001 | 0.0049 | 0 | 0.0115 | 0.0122 | 0.0016 | 0 | 0.0017 | 0 | 0.0178 | 0 | 0.0017 | 0 | 0.0022 | 0 | 0.032 | 0 |
Lipid Particles | 0.0043 | 0 | 0.0069 | 0 | 0.0148 | 0.0061 | 0.0068 | 0 | 0.0008 | 0 | 0.0182 | 0 | 0.0004 | 0 | 0.0059 | 0 | 0.0107 | 0 |
Mitochondria | 0.0136 | 0.0002 | 0.0059 | 0.0002 | 0.0388 | 0.0056 | 0.0017 | 0.0002 | 0.0015 | 0.0002 | 0.072 | 0.0002 | 0.0021 | 0.0001 | 0.0012 | 0.0002 | 0.0108 | 0.0002 |
None | 0.0035 | 0.0009 | 0.0011 | 0.0011 | 0.0038 | 0.0009 | 0.0033 | 0.0004 | 0.0008 | 0.0005 | 0.0019 | 0.0006 | 0.0026 | 0.0012 | 0.0319 | 0.0008 | 0.0035 | 0.0012 |
Nuclear Periphery | 0.0365 | 0.0086 | 0.0126 | 0.0082 | 0.0104 | 0.0257 | 0.0281 | 0.007 | 0.0078 | 0.0118 | 0.0115 | 0.0092 | 0.0145 | 0.0042 | 0.0053 | 0.0052 | 0.0166 | 0.0097 |
Nucleolus | 0.0007 | 0.0001 | 0.0002 | 0 | 0.0037 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0013 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0001 |
Nucleus | 0.2578 | 0.1727 | 0.1899 | 0.2603 | 0.1289 | 0.242 | 0.2006 | 0.1453 | 0.1478 | 0.2226 | 0.1393 | 0.2316 | 0.1722 | 0.0999 | 0.101 | 0.1708 | 0.073 | 0.2017 |
Peroxisomes | 0.0018 | 0.0001 | 0.0008 | 0 | 0.009 | 0.0007 | 0.0016 | 0 | 0.0015 | 0 | 0.0109 | 0 | 0.0003 | 0 | 0.0087 | 0 | 0.0019 | 0 |
Punctate Nuclear | 0.0226 | 0.0014 | 0.0021 | 0.0009 | 0.0053 | 0.0023 | 0.0072 | 0.0015 | 0.0016 | 0.0017 | 0.0031 | 0.001 | 0.0027 | 0.002 | 0.0029 | 0.0031 | 0.0028 | 0.0022 |
Vacuole | 0.0023 | 0.0008 | 0.0013 | 0.0003 | 0.0179 | 0.002 | 0.0015 | 0.0003 | 0.0005 | 0.0007 | 0.0122 | 0.0003 | 0.0009 | 0.0002 | 0.0005 | 0.0002 | 0.0017 | 0.0005 |
Vacuole Periphery | 0.0018 | 0.0001 | 0.0005 | 0.0001 | 0.0046 | 0.0018 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0058 | 0.0001 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0022 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 153.7222 | 131.5168 | 112.6052 | 114.6363 | 119.829 | 156.4647 | 123.1738 | 123.6942 | 166.9742 | 112.7122 |
Translational Efficiency | 1.7513 | 1.8823 | 1.9441 | 2.6317 | 2.3359 | 1.981 | 1.6487 | 2.3117 | 1.7548 | 2.0857 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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