Standard name | |
---|---|
Human Ortholog | |
Description | Peroxisomal membrane protein (PMP); required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 13 | 7 | 7 | 3 | 2 | 10 | 36 | – | 13 | 16 | 15 | 45 | 67 | 32 | 7 | 3 | 7 | 0 | 12 | 6 | 10 | 21 | 25 |
Bud | 4 | 39 | 42 | 56 | 77 | 85 | 147 | 167 | – | 177 | 161 | 200 | 44 | 83 | 61 | 13 | 20 | 18 | 3 | 15 | 5 | 14 | 20 | 15 |
Bud Neck | 0 | 5 | 6 | 1 | 6 | 9 | 7 | 6 | – | 8 | 6 | 2 | 9 | 12 | 9 | 2 | 7 | 3 | 1 | 14 | 9 | 16 | 11 | 16 |
Bud Site | 6 | 44 | 38 | 36 | 43 | 63 | 56 | 43 | – | 84 | 71 | 62 | 31 | 50 | 16 | 18 | 28 | 13 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 2 | 9 | 3 | 2 | 4 | 14 | 18 | – | 5 | 3 | 5 | 11 | 10 | 13 | 10 | 21 | 11 | 0 | 1 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 4 | 0 | 1 | 0 | 0 | 1 | 2 |
Endosome | 3 | 8 | 31 | 12 | 58 | 32 | 34 | 42 | – | 6 | 9 | 31 | 34 | 40 | 47 | 11 | 31 | 13 | 9 | 26 | 33 | 56 | 46 | 69 |
Golgi | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 3 | 6 | 9 | 1 | 1 | 1 | 0 | 6 | 1 | 4 | 5 | 11 |
Mitochondria | 22 | 168 | 90 | 84 | 100 | 182 | 194 | 178 | – | 300 | 277 | 264 | 83 | 85 | 62 | 21 | 23 | 15 | 4 | 9 | 3 | 6 | 14 | 15 |
Nucleus | 0 | 1 | 0 | 1 | 7 | 5 | 6 | 4 | – | 4 | 5 | 4 | 5 | 8 | 6 | 6 | 11 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | – | 4 | 1 | 2 | 2 | 1 | 0 | 11 | 7 | 8 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 4 | 13 | 17 | 16 | 17 | 28 | 30 | 23 | – | 28 | 23 | 45 | 14 | 12 | 15 | 17 | 37 | 21 | 0 | 3 | 0 | 2 | 1 | 1 |
Peroxisomes | 40 | 171 | 349 | 275 | 425 | 441 | 607 | 570 | – | 546 | 418 | 635 | 342 | 438 | 470 | 109 | 174 | 131 | 68 | 189 | 313 | 446 | 709 | 802 |
SpindlePole | 27 | 52 | 186 | 129 | 253 | 244 | 394 | 324 | – | 246 | 232 | 321 | 294 | 374 | 319 | 51 | 101 | 56 | 16 | 52 | 97 | 94 | 80 | 107 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 3 | – | 2 | 0 | 1 | 2 | 4 | 1 | 0 | 4 | 3 | 0 | 0 | 1 | 0 | 1 | 4 |
Unique Cell Count | 73 | 317 | 472 | 374 | 592 | 654 | 933 | 903 | 832 | 727 | 948 | 636 | 778 | 770 | 187 | 327 | 225 | 110 | 342 | 481 | 658 | 921 | 1080 | |
Labelled Cell Count | 108 | 518 | 776 | 620 | 992 | 1099 | 1501 | 1415 | 1423 | 1223 | 1588 | 919 | 1190 | 1060 | 279 | 473 | 305 | 110 | 342 | 481 | 658 | 921 | 1080 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.3 | 6.7 | 6.2 | 6.0 | 5.8 | 5.3 | 5.1 | 5.7 | – | 4.9 | 5.0 | 5.2 | 7.7 | 7.4 | 8.2 | 6.5 | 8.0 | 9.1 | 7.4 | 7.7 | 7.8 |
Std Deviation (1e-4) | 1.6 | 1.3 | 1.3 | 1.2 | 1.2 | 1.4 | 1.3 | 1.3 | – | 1.2 | 1.2 | 1.2 | 1.2 | 1.1 | 1.2 | 2.0 | 2.5 | 2.8 | 1.4 | 1.6 | 1.6 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.1 | -0.22 | -0.27 | -0.12 | – | -0.34 | -0.3 | -0.26 | 0.32 | 0.27 | 0.4 | 0.08 | 0.37 | 0.56 | 0.27 | 0.32 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.4 | 0 | 0 | -0.7 | 2.5 | – | 0.1 | 0.9 | 0.1 | 4.4 | 5.2 | 2.6 | 0 | 0 | 0 |
Bud | 2.7 | 2.1 | 2.1 | 3.6 | 4.7 | – | 5.7 | 6.0 | 5.8 | -1.2 | 1.0 | -0.6 | -0.8 | -1.4 | -0.4 |
Bud Neck | 0 | -0.4 | 0.2 | 0 | 0 | – | -0.5 | 0 | 0 | 0.2 | 0.4 | -0.2 | 0 | 1.0 | 0 |
Bud Site | 0.8 | -0.5 | 0.9 | -1.5 | -2.5 | – | 1.2 | 1.0 | -1.0 | -2.2 | -1.1 | -5.0 | 0.7 | 0.3 | -1.1 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.3 | 0 | -2.0 | -0.6 | 0.1 | – | -2.2 | 0 | 0 | -0.2 | -0.9 | -0.3 | 2.4 | 3.3 | 2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -2.2 | 1.9 | -1.2 | -2.5 | -1.5 | – | -6.1 | -5.0 | -2.9 | -0.9 | -1.1 | -0.3 | -0.3 | 1.5 | -0.4 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.2 | -0.9 | 3.4 | 0.8 | 0.3 | – | 6.4 | 7.0 | 3.6 | -2.7 | -4.0 | -5.7 | -2.4 | -4.8 | -4.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.5 | -0.7 | 0.6 | -0.4 | -1.1 | – | -0.2 | -0.4 | 1.0 | -1.4 | -2.3 | -1.8 | 2.9 | 4.3 | 3.1 |
Peroxisomes | -0.1 | -0.8 | -2.4 | -3.4 | -4.0 | – | -3.1 | -5.8 | -2.7 | -6.9 | -6.3 | -4.7 | -3.9 | -6.1 | -4.2 |
SpindlePole | -1.5 | 1.1 | -0.7 | 1.0 | -1.3 | – | -3.6 | -2.7 | -2.1 | 2.3 | 3.0 | 0.7 | -2.9 | -2.5 | -3.8 |
Vacuole | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.7721 | 3.5805 | 3.9144 | 3.3616 | 2.7077 | 4.0163 | 3.6199 | 4.4069 | 4.5463 | 4.3036 | 3.6685 | 4.7483 | 3.5395 | 4.0919 | 4.0602 | 3.9082 | 3.3531 | 4.0585 |
Actin | 0.0202 | 0.021 | 0.0236 | 0.0006 | 0.0047 | 0.0074 | 0.0222 | 0.0194 | 0.0239 | 0.0015 | 0.0016 | 0.0223 | 0.0084 | 0.0501 | 0.0124 | 0.0059 | 0.0011 | 0.0029 |
Bud | 0.0009 | 0.0049 | 0.0053 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0027 | 0.0026 | 0.0002 | 0.0016 | 0.0005 | 0.0001 | 0.0084 | 0.0003 | 0.0001 | 0.0001 | 0.0001 |
Bud Neck | 0.0028 | 0.0016 | 0.0175 | 0.0007 | 0.0001 | 0.0019 | 0.0018 | 0.0154 | 0.02 | 0.014 | 0.0003 | 0.0015 | 0.0012 | 0.006 | 0.0011 | 0.0002 | 0.0003 | 0.0035 |
Bud Periphery | 0.0009 | 0.0051 | 0.0054 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0034 | 0.0032 | 0.0002 | 0.0021 | 0.0014 | 0.0001 | 0.0067 | 0.0004 | 0.0001 | 0.0001 | 0.0001 |
Bud Site | 0.0278 | 0.0583 | 0.0142 | 0.0003 | 0.0004 | 0.0003 | 0.0014 | 0.0632 | 0.0221 | 0.0006 | 0.0082 | 0.0008 | 0.0012 | 0.0632 | 0.0022 | 0.0007 | 0.0002 | 0.0002 |
Cell Periphery | 0.0131 | 0.0143 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0.0003 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.0062 | 0.0014 | 0.0001 | 0 | 0.0001 | 0.0168 | 0.0003 | 0.0002 | 0.0007 | 0.0001 | 0.0337 | 0.0001 | 0.0027 | 0.0015 | 0.0003 | 0.0002 | 0 | 0.0001 |
Cytoplasmic Foci | 0.1416 | 0.1724 | 0.1248 | 0.2157 | 0.0977 | 0.0969 | 0.1474 | 0.162 | 0.1223 | 0.2131 | 0.1415 | 0.1224 | 0.1149 | 0.1597 | 0.1537 | 0.1784 | 0.1401 | 0.1668 |
Eisosomes | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.001 | 0.0081 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0003 | 0 | 0.0012 | 0.0002 | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 |
Endosome | 0.0103 | 0.005 | 0.0029 | 0.0009 | 0.0061 | 0.0018 | 0.0171 | 0.0043 | 0.0102 | 0.0037 | 0.0065 | 0.0045 | 0.0157 | 0.0072 | 0.0044 | 0.0051 | 0.0027 | 0.0064 |
Golgi | 0.0117 | 0.01 | 0.0141 | 0.0053 | 0.0144 | 0.0076 | 0.0159 | 0.0094 | 0.0147 | 0.0096 | 0.0112 | 0.013 | 0.013 | 0.0145 | 0.0099 | 0.0106 | 0.0113 | 0.0113 |
Lipid Particles | 0.1511 | 0.081 | 0.0994 | 0.0605 | 0.2666 | 0.141 | 0.1228 | 0.1155 | 0.0774 | 0.1916 | 0.1926 | 0.195 | 0.1557 | 0.064 | 0.1009 | 0.2142 | 0.074 | 0.1541 |
Mitochondria | 0.0047 | 0.0145 | 0.0264 | 0.0036 | 0.0092 | 0.011 | 0.0165 | 0.0209 | 0.0234 | 0.0295 | 0.0449 | 0.016 | 0.0061 | 0.0122 | 0.0127 | 0.0116 | 0.0023 | 0.0125 |
None | 0.0026 | 0.0003 | 0.0001 | 0 | 0.0001 | 0.017 | 0.0004 | 0.0002 | 0.0016 | 0 | 0.0012 | 0.0001 | 0.0055 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 |
Nuclear Periphery | 0.0066 | 0.0007 | 0 | 0 | 0 | 0 | 0.0026 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0003 | 0.0012 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0005 |
Nucleolus | 0.0017 | 0.0082 | 0.0011 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.006 | 0.0024 | 0.0007 | 0.0029 | 0.0028 | 0.0012 | 0.0029 | 0.0075 | 0.0002 | 0.0001 | 0.0005 |
Nucleus | 0.0025 | 0.0013 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0.0005 | 0.0002 | 0.0005 | 0.0101 | 0.0019 | 0 | 0 | 0.0001 |
Peroxisomes | 0.5728 | 0.5786 | 0.6618 | 0.7052 | 0.5988 | 0.6744 | 0.6428 | 0.5594 | 0.6611 | 0.5219 | 0.5312 | 0.6142 | 0.6522 | 0.5704 | 0.678 | 0.5694 | 0.7664 | 0.6204 |
Punctate Nuclear | 0.0176 | 0.0122 | 0.0021 | 0.0069 | 0.0013 | 0.023 | 0.0061 | 0.0132 | 0.0102 | 0.0129 | 0.0159 | 0.0033 | 0.0187 | 0.0185 | 0.0115 | 0.0022 | 0.0011 | 0.0199 |
Vacuole | 0.0034 | 0.0009 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0004 | 0.0031 | 0.002 | 0.0002 | 0.0017 | 0.0009 | 0.0004 | 0.0025 | 0.0018 | 0.0001 | 0 | 0.0002 |
Vacuole Periphery | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0004 | 0.0007 | 0.0001 | 0.0005 | 0.0003 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 16.6426 | 13.3897 | 17.1116 | 18.9142 | 14.0256 | 14.97 | 19.1793 | 21.5746 | 21.7285 | 21.1026 |
Translational Efficiency | 0.9063 | 1.0027 | 0.8013 | 0.7819 | 0.9466 | 1.0132 | 0.9008 | 0.7796 | 0.8042 | 0.7783 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
164 | 822 | 331 | 1346 | 1750 | 925 | 2583 | 1448 | 1914 | 1747 | 2914 | 2794 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 979.18 | 875.66 | 1113.18 | 1030.60 | 727.56 | 849.23 | 997.48 | 995.60 | 749.12 | 861.67 | 1010.62 | 1012.46 |
Standard Deviation | 157.20 | 151.15 | 135.16 | 163.39 | 123.08 | 115.34 | 127.27 | 151.44 | 144.67 | 134.05 | 133.34 | 158.28 |
Intensity Change Log 2 | — | -0.161203 | 0.185041 | 0.073839 | — | 0.223089 | 0.455222 | 0.452500 | — | 0.015261 | 0.306451 | 0.247533 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.137016* | 0.020710 | 0.046578 | 0.234884* | 0.006451 | 0.009902 | 0.026320 | 0.179002* | 0.017639 | 0.014988 | 0.028621 | 0.205923* |
Bud Neck | 0.004175 | 0.005743 | 0.011573 | 0.003255 | 0.008148 | 0.003749 | 0.003725 | 0.003455 | 0.007807 | 0.004687 | 0.004616 | 0.003359 |
Bud Site | 0.016574 | 0.025652 | 0.061631 | 0.025269 | 0.018533 | 0.013874 | 0.023172 | 0.016686 | 0.018365 | 0.019416 | 0.027541 | 0.020821 |
Cell Periphery | 0.000218 | 0.000282 | 0.000170 | 0.000179 | 0.000766 | 0.000127 | 0.000086 | 0.000154 | 0.000719 | 0.000200 | 0.000095 | 0.000166 |
Cytoplasm | 0.003276 | 0.005869 | 0.007682 | 0.005862 | 0.008661 | 0.003705 | 0.009554 | 0.006394 | 0.008200 | 0.004723 | 0.009341 | 0.006138 |
Cytoplasmic Foci | 0.069848 | 0.099304 | 0.143684 | 0.048736 | 0.123392 | 0.077552 | 0.072579 | 0.050805 | 0.118804 | 0.087786 | 0.080656 | 0.049808 |
Eisosomes | 0.000345 | 0.000656 | 0.000464 | 0.001611 | 0.000562 | 0.000243 | 0.000276 | 0.001812 | 0.000543 | 0.000437 | 0.000297 | 0.001715 |
Endoplasmic Reticulum | 0.000940 | 0.000452 | 0.000794 | 0.000260 | 0.000214 | 0.000141 | 0.000075 | 0.000175 | 0.000276 | 0.000288 | 0.000156 | 0.000216 |
Endosome | 0.060100 | 0.025616 | 0.027158 | 0.018753 | 0.009646 | 0.032699 | 0.008544 | 0.013283 | 0.013969 | 0.029367 | 0.010659 | 0.015918 |
Golgi | 0.065732 | 0.041338 | 0.020663 | 0.030159 | 0.030969 | 0.053352 | 0.016478 | 0.025137 | 0.033947 | 0.047699 | 0.016953 | 0.027557 |
Lipid Particles | 0.022479 | 0.063881 | 0.022937 | 0.022998 | 0.028852 | 0.038516 | 0.018537 | 0.027950 | 0.028306 | 0.050450 | 0.019036 | 0.025565 |
Mitochondria | 0.011945 | 0.013278 | 0.002607 | 0.013936 | 0.010887 | 0.018962 | 0.008977 | 0.013202 | 0.010977 | 0.016288 | 0.008253 | 0.013556 |
Mitotic Spindle | 0.014549 | 0.013239 | 0.082759 | 0.014846 | 0.004469 | 0.013580 | 0.031563 | 0.008931 | 0.005332 | 0.013420 | 0.037379 | 0.011780 |
None | 0.000045 | 0.000526 | 0.000062 | 0.000083 | 0.001654 | 0.000074 | 0.000042 | 0.000094 | 0.001516 | 0.000287 | 0.000044 | 0.000088 |
Nuclear Periphery | 0.000088 | 0.000077 | 0.000191 | 0.000064 | 0.000022 | 0.000045 | 0.000070 | 0.000061 | 0.000027 | 0.000060 | 0.000083 | 0.000062 |
Nuclear Periphery Foci | 0.000405 | 0.001263 | 0.005644 | 0.000182 | 0.001529 | 0.000458 | 0.000367 | 0.000590 | 0.001432 | 0.000837 | 0.000966 | 0.000393 |
Nucleolus | 0.000036 | 0.000980 | 0.001030 | 0.000030 | 0.000534 | 0.000159 | 0.000108 | 0.000069 | 0.000491 | 0.000545 | 0.000213 | 0.000051 |
Nucleus | 0.000084 | 0.000713 | 0.000947 | 0.000045 | 0.000639 | 0.000082 | 0.000037 | 0.000049 | 0.000592 | 0.000379 | 0.000140 | 0.000047 |
Peroxisomes | 0.584388* | 0.677957* | 0.556974* | 0.576063* | 0.743155* | 0.731403* | 0.778800* | 0.651029* | 0.729551* | 0.706256* | 0.753603* | 0.614915* |
Vacuole | 0.002426 | 0.002113 | 0.006236 | 0.001927 | 0.000559 | 0.000717 | 0.000565 | 0.001039 | 0.000719 | 0.001374 | 0.001209 | 0.001467 |
Vacuole Periphery | 0.005332 | 0.000351 | 0.000215 | 0.000857 | 0.000359 | 0.000659 | 0.000126 | 0.000083 | 0.000785 | 0.000514 | 0.000136 | 0.000456 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 5.88 | 4.40 | -4.60 | -25.14* | -18.27 | -2.37 | -11.37 | -23.77* | -23.08* | -20.66* | 1.11 | -4.30 | -32.44* | -34.02* | -31.25* |
Bud Neck | -0.99 | -3.87 | 0.60 | 3.47 | 6.06 | 3.10 | 3.28 | 3.41 | 0.38 | 0.58 | 2.47 | 2.51 | 3.62 | 2.54 | 3.09 |
Bud Site | -1.64 | -5.26 | -1.76 | 0.03 | 4.66 | 1.54 | -1.88 | 0.67 | -1.03 | 2.79 | -0.37 | -3.78 | -1.09 | -0.64 | 3.07 |
Cell Periphery | -1.04 | 0.84 | 0.82 | 1.87 | 0.01 | 4.53 | 4.82 | 4.34 | -1.52 | -4.77 | 3.97 | 4.82 | 4.28 | 1.23 | -5.23 |
Cytoplasm | -2.64 | -2.60 | -2.98 | -0.09 | 1.13 | 5.14 | -0.62 | 2.00 | -2.99 | 3.14 | 3.85 | -0.99 | 2.16 | -2.25 | 4.17 |
Cytoplasmic Foci | -3.03 | -5.48 | 2.43 | 8.69 | 8.62 | 7.51 | 9.20 | 13.07 | 5.73 | 5.88 | 5.68 | 7.27 | 14.22 | 10.28 | 9.67 |
Eisosomes | -3.97 | -1.34 | -4.34 | -3.27 | -3.94 | 3.46 | 3.24 | -5.13 | -6.83 | -6.73 | 1.25 | 3.05 | -5.91 | -6.94 | -7.78 |
Endoplasmic Reticulum | 1.76 | -0.17 | 2.67 | 2.57 | 1.90 | 1.24 | 2.74 | 0.81 | -0.94 | -5.61 | -0.31 | 1.42 | 1.18 | 2.04 | -0.72 |
Endosome | 3.41 | 3.10 | 4.18 | 2.16 | 2.20 | -8.31 | 1.17 | -2.72 | 6.71 | -3.80 | -6.91 | 2.48 | -1.34 | 6.28 | -4.53 |
Golgi | 2.47 | 4.62 | 3.61 | 3.85 | -3.45 | -7.88 | 9.63 | 2.99 | 9.61 | -5.11 | -6.21 | 10.49 | 3.37 | 9.69 | -7.32 |
Lipid Particles | -5.06 | -0.09 | -0.10 | 6.65 | 0.00 | -2.28 | 3.64 | 0.29 | 2.50 | -3.19 | -5.48 | 3.49 | 1.00 | 6.76 | -3.05 |
Mitochondria | -0.36 | 3.23 | -0.61 | -0.24 | -6.37 | -2.72 | 1.17 | -1.19 | 1.93 | -2.54 | -2.43 | 1.82 | -1.55 | 1.31 | -3.93 |
Mitotic Spindle | 0.17 | -5.88 | 0.05 | -0.25 | 6.58 | -3.88 | -12.20 | -2.93 | 1.80 | 9.27 | -4.58 | -13.55 | -4.50 | 1.04 | 10.52 |
None | -2.05 | -1.75 | -4.46 | 1.90 | -0.51 | 3.68 | 3.77 | 3.66 | -0.79 | -6.83 | 3.02 | 3.76 | 3.66 | 1.84 | -8.13 |
Nuclear Periphery | 0.20 | -2.23 | 0.48 | 0.71 | 4.00 | -3.80 | -9.98 | -2.39 | -0.91 | 0.59 | -3.52 | -8.12 | -3.34 | -0.17 | 2.29 |
Nuclear Periphery Foci | -2.79 | -2.78 | 1.24 | 3.84 | 2.86 | 2.55 | 3.48 | 2.08 | -0.14 | -0.79 | 1.60 | 0.47 | 3.04 | 2.12 | 2.33 |
Nucleolus | -1.71 | -1.58 | 0.43 | 1.72 | 1.58 | 1.10 | 2.19 | 2.73 | 1.09 | 0.79 | -0.30 | 1.16 | 2.86 | 2.00 | 1.88 |
Nucleus | -2.54 | -1.95 | 0.73 | 2.70 | 2.00 | 2.66 | 2.96 | 2.92 | 1.36 | -0.34 | 0.96 | 1.99 | 2.94 | 2.87 | 1.87 |
Peroxisomes | -2.97 | 1.20 | 0.29 | 7.19 | -1.58 | 1.00 | -4.09 | 8.38 | 6.41 | 12.72 | 2.38 | -2.55 | 12.35 | 9.66 | 16.74 |
Vacuole | 0.19 | -2.04 | 0.16 | -0.06 | 2.15 | -0.95 | -0.12 | -1.00 | -0.73 | -0.98 | -1.48 | -2.12 | -2.19 | -0.42 | -0.42 |
Vacuole Periphery | 1.52 | 1.55 | 1.35 | -1.45 | -1.84 | -0.79 | 0.73 | 0.89 | 2.66 | 0.60 | 0.64 | 1.59 | 0.76 | 0.27 | -1.86 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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