Standard name
Human Ortholog
Description Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Rkr1p-Tae2p-Cdc48p-Npl4p-Ufd1p) is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Rkr1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.05 0 0.06 0.05 0.05 0.07 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 0.99 0.99 0.96 0.95 0.95 0.87 0.86 0.88 0.85 1.0 0.99 1.0 0.92 0.9 0.9 0.85 0.88 0.78 0.71 0.73 0.76
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.06 0 0.2 0.19 0.23 0.15 0 0 0 0.08 0 0.1 0.07 0 0.1 0.19 0.11 0.09
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 2 1 0 0 0 1 0 0 0 0 0 0 0 0 0 2 1 3 1 3 4
Bud 0 0 0 1 1 0 3 2 6 3 10 3 0 0 0 0 0 0 0 1 1 0 6 7
Bud Neck 0 6 23 11 23 21 27 25 13 11 2 19 0 1 0 2 0 0 0 0 1 1 5 2
Bud Site 0 0 0 0 0 1 1 0 1 1 1 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 19 9 2 1 3 2 2 2 1 2 0 2 0 2 4 1 0 2 7 1 4 2
Cytoplasm 102 346 425 375 363 407 502 344 302 299 257 263 305 350 318 44 60 35 102 324 293 113 211 212
Endoplasmic Reticulum 1 1 2 1 2 1 0 0 0 0 0 0 0 2 2 4 3 4 0 3 1 4 1 1
Endosome 0 0 1 1 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 4 0 2 2
Golgi 0 0 0 0 0 0 0 1 0 0 0 0 0 1 1 0 2 0 0 1 0 0 2 1
Mitochondria 1 3 2 0 0 14 30 10 68 67 68 48 1 0 2 4 3 4 8 13 36 30 32 23
Nucleus 0 1 0 2 2 0 0 1 3 2 1 1 0 0 1 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 7 8 6 5 11 2 11 0 0 0 1 1 0 1 1 5 1 3 0
Vac/Vac Membrane 0 1 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 0 3 2 2 9 13
Unique Cell Count 105 353 432 380 368 426 529 363 346 348 293 310 305 353 319 48 67 39 121 368 378 160 290 278
Labelled Cell Count 106 358 473 402 396 452 575 393 402 397 342 349 306 356 324 57 75 45 121 368 378 160 290 278


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.9 3.7 4.7 4.1 4.7 3.4 3.6 3.9 3.3 3.0 3.0 3.5 4.0 4.1 4.3 5.3 5.9 4.9 3.5 3.9 4.5
Std Deviation (1e-4) 0.6 0.5 0.9 1.1 1.4 0.8 0.9 1.0 1.4 1.1 1.1 0.9 1.5 1.6 1.9 1.0 1.7 1.0 1.2 2.0 2.4
Intensity Change (Log2) – – – -0.17 0.0 -0.45 -0.36 -0.25 -0.51 -0.63 -0.63 -0.43 -0.21 -0.19 -0.1 0.17 0.34 0.08 -0.41 -0.25 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -1.2101 -0.9595 -1.1495 -1.2659 -1.3534 -0.9209 -0.5336 -0.6176 -0.8238 -0.7729 -0.9806 -0.6116 -0.8869 -1.1139 -1.0599 -1.0039 -1.2423 -1.0042
Actin 0.0267 0.0015 0.0016 0.0011 0.0006 0.0005 0.0419 0.0006 0.0073 0.0002 0.0026 0.0031 0.0354 0.0003 0.0111 0.0204 0.0017 0.0079
Bud 0.0059 0.0009 0.0034 0.0008 0.0002 0.0002 0.0053 0.0025 0.0035 0.0001 0.0003 0.001 0.0017 0.0013 0.0014 0.0063 0.0052 0.0006
Bud Neck 0.0087 0.0003 0.0002 0.0001 0.0001 0.0003 0.0039 0.0003 0.001 0.0001 0.0002 0.0051 0.0012 0.0008 0.0002 0.0092 0.0006 0.0069
Bud Periphery 0.0068 0.0013 0.0042 0.0021 0.0008 0.0002 0.0087 0.003 0.0051 0.0001 0.0008 0.003 0.004 0.0007 0.0021 0.0205 0.0111 0.0012
Bud Site 0.0051 0.0071 0.0102 0.0001 0.0001 0.0001 0.0115 0.0154 0.0059 0.0001 0.0004 0.0006 0.0055 0.024 0.0062 0.0132 0.0008 0.0013
Cell Periphery 0.0004 0.0003 0.0004 0.0001 0.0001 0 0.0005 0.0008 0.0066 0 0.0001 0.0007 0.0005 0.0003 0.0002 0.0006 0.0003 0.0001
Cytoplasm 0.0292 0.0526 0.0428 0.0365 0.0364 0.0713 0.0979 0.1554 0.1391 0.1656 0.1851 0.2283 0.019 0.0653 0.0598 0.0333 0.1402 0.0789
Cytoplasmic Foci 0.0055 0.0061 0.0097 0.0055 0.0081 0.0193 0.0195 0.0065 0.0094 0.0125 0.0221 0.0085 0.0158 0.0079 0.0099 0.0106 0.0068 0.012
Eisosomes 0.0005 0.0003 0.0003 0.0003 0.0001 0.0001 0.0006 0.0002 0.0002 0.0001 0.0001 0.0001 0.0006 0.0002 0.0004 0.0003 0.0001 0.0001
Endoplasmic Reticulum 0.0018 0.0007 0.0014 0.0006 0.0004 0.0003 0.0041 0.0009 0.0008 0.0009 0.0015 0.0014 0.0056 0.0008 0.0005 0.0008 0.0011 0.0007
Endosome 0.0041 0.0007 0.0037 0.0008 0.0011 0.0012 0.0142 0.0011 0.0025 0.0207 0.007 0.0027 0.0136 0.0032 0.0016 0.0034 0.021 0.0045
Golgi 0.0014 0.0001 0.0013 0.0002 0.0001 0.0001 0.0055 0.0002 0.0008 0.0003 0.0024 0.0006 0.0091 0.0005 0.0033 0.0048 0.0127 0.0035
Lipid Particles 0.0016 0.0004 0.0021 0.0006 0.0003 0.0003 0.0107 0.0004 0.0021 0.0001 0.0016 0.0019 0.0068 0.0028 0.0026 0.0016 0.0033 0.0045
Mitochondria 0.0009 0.0003 0.0051 0.0008 0.0002 0.0002 0.0042 0.0005 0.0015 0.0003 0.02 0.0023 0.0062 0.0007 0.0054 0.0017 0.0106 0.0023
None 0.8938 0.9221 0.9059 0.9469 0.9495 0.9024 0.7357 0.8082 0.8082 0.7951 0.7455 0.7258 0.8287 0.886 0.8773 0.8655 0.7771 0.8526
Nuclear Periphery 0.0017 0.0005 0.0008 0.0006 0.0002 0.0002 0.0093 0.0004 0.0004 0.0006 0.0012 0.0027 0.0139 0.0006 0.0002 0.0005 0.0007 0.0011
Nucleolus 0.0004 0.0005 0.0006 0.0003 0.0001 0.0001 0.0046 0.0003 0.0004 0.0001 0.0003 0.0004 0.0016 0.0004 0.0002 0.0004 0.0003 0.0006
Nucleus 0.0012 0.001 0.0018 0.0005 0.0003 0.0003 0.0044 0.0009 0.0011 0.0006 0.0023 0.0016 0.0041 0.0014 0.0004 0.0006 0.0006 0.0015
Peroxisomes 0.0013 0.0001 0.0013 0.0005 0.0003 0.0005 0.0088 0.0008 0.0022 0.0001 0.0007 0.0054 0.0111 0.0004 0.0163 0.004 0.001 0.0165
Punctate Nuclear 0.0013 0.0027 0.002 0.0009 0.0005 0.0015 0.0047 0.0008 0.0013 0.0004 0.0032 0.0038 0.0077 0.0016 0.0005 0.001 0.0009 0.0025
Vacuole 0.0012 0.0005 0.0007 0.0006 0.0006 0.0004 0.0026 0.0009 0.0008 0.0017 0.0016 0.0009 0.0052 0.0007 0.0003 0.001 0.0023 0.0005
Vacuole Periphery 0.0003 0.0001 0.0002 0.0002 0.0001 0.0001 0.0015 0.0001 0.0002 0.0006 0.001 0.0003 0.0027 0.0001 0.0001 0.0002 0.0016 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 23.1146 19.9818 21.9041 28.2971 19.1012 23.3557 28.9279 28.2019 25.1554 31.1678
Translational Efficiency 0.9689 1.3142 1.1054 0.8449 1.1404 1.1393 0.9168 0.8163 0.9564 0.9341

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
143 392 196 1320 1490 1227 165 1583 1633 1619 361 2903

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 625.10 712.00 947.06 794.84 636.14 687.93 919.96 808.62 635.17 693.76 934.67 802.35
Standard Deviation 57.81 90.50 111.49 106.05 69.53 80.04 85.67 110.28 68.65 83.33 101.42 108.59
Intensity Change Log 2 — 0.187790 0.599369 0.346577 — 0.112917 0.532227 0.346118 — 0.150512 0.565895 0.346346

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000036 0.000460 0.001066 0.000649 0.000095 0.000730 0.000495 0.000336 0.000090 0.000665 0.000805 0.000478
Bud Neck 0.031133 0.037769 0.011740 0.006363 0.019688 0.048471 0.001675 0.003642 0.020691 0.045880 0.007139 0.004879
Bud Site 0.002833 0.025562 0.023914 0.026970 0.002970 0.017062 0.002219 0.030374 0.002958 0.019120 0.013998 0.028827
Cell Periphery 0.000230 0.000120 0.000317 0.000135 0.000096 0.000147 0.000290 0.000145 0.000108 0.000141 0.000304 0.000140
Cytoplasm 0.425206* 0.356912* 0.159123* 0.553090* 0.461536* 0.330286* 0.195266* 0.617450* 0.458354* 0.336733* 0.175642* 0.588186*
Cytoplasmic Foci 0.309596* 0.267394* 0.014555 0.044113 0.242087* 0.315186* 0.019984 0.033709 0.247999* 0.303615* 0.017037 0.038440
Eisosomes 0.000318 0.000129 0.000142 0.000049 0.000144 0.000193 0.000155 0.000038 0.000160 0.000177 0.000148 0.000043
Endoplasmic Reticulum 0.000188 0.000489 0.014995 0.000944 0.000792 0.000467 0.007732 0.001062 0.000740 0.000472 0.011675 0.001008
Endosome 0.019577 0.040260 0.006370 0.009506 0.016235 0.046664 0.007056 0.006831 0.016528 0.045114 0.006683 0.008047
Golgi 0.001961 0.004908 0.000173 0.003180 0.001730 0.010959 0.000128 0.002925 0.001750 0.009494 0.000152 0.003041
Lipid Particles 0.011919 0.005699 0.005418 0.001339 0.010979 0.009180 0.005757 0.000876 0.011061 0.008337 0.005573 0.001086
Mitochondria 0.009864 0.020964 0.000602 0.006862 0.005674 0.025993 0.000347 0.001362 0.006041 0.024775 0.000486 0.003863
Mitotic Spindle 0.000026 0.008222 0.006591 0.023999 0.000968 0.006257 0.000316 0.023424 0.000886 0.006733 0.003723 0.023685
None 0.000829 0.002350 0.002321 0.003719 0.002029 0.001890 0.002974 0.004523 0.001924 0.002002 0.002620 0.004158
Nuclear Periphery 0.000312 0.000422 0.002887 0.000724 0.000749 0.000606 0.002208 0.000655 0.000710 0.000561 0.002577 0.000686
Nuclear Periphery Foci 0.000171 0.000589 0.004160 0.000956 0.000261 0.000381 0.003681 0.000847 0.000253 0.000431 0.003941 0.000896
Nucleolus 0.000763 0.001809 0.001030 0.000973 0.001660 0.002586 0.001015 0.000264 0.001581 0.002398 0.001023 0.000587
Nucleus 0.128889 0.092507 0.597603* 0.087784 0.167435* 0.062838 0.568454* 0.107511 0.164060* 0.070022 0.584280* 0.098541
Peroxisomes 0.007243 0.035902 0.000716 0.001766 0.005573 0.027478 0.000580 0.001109 0.005719 0.029518 0.000654 0.001408
Vacuole 0.047887 0.094817 0.145941 0.222193* 0.058265 0.089346 0.179400 0.160152 0.057356 0.090671 0.161234 0.188362
Vacuole Periphery 0.001020 0.002716 0.000337 0.004685 0.001033 0.003278 0.000268 0.002764 0.001032 0.003142 0.000306 0.003638

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.52 -11.01 -2.53 0.65 5.35 -2.30 -7.87 -2.02 1.65 3.46 -2.78 -12.34 -2.93 1.59 6.62
Bud Neck -0.59 3.21 4.09 6.70 1.16 -8.46 10.70 10.22 14.95 -2.23 -8.30 6.65 10.63 16.21 0.95
Bud Site -5.50 -2.35 -6.06 2.08 0.48 -9.39 -0.05 -7.25 -0.58 -7.26 -10.65 -2.32 -9.41 0.83 -1.37
Cell Periphery 2.61 -2.19 3.42 1.81 10.01 -3.65 -8.32 -0.22 2.41 6.95 -2.75 -12.10 1.23 3.24 11.79
Cytoplasm 2.90 12.73 -2.02 -6.87 -23.27 12.72 20.07 -7.17 -18.95 -24.44 13.00 28.50 -5.38 -19.06 -34.25
Cytoplasmic Foci 2.29 17.33 16.13 20.87 -8.47 -8.51 39.40 39.73 42.94 -3.78 -7.04 44.43 42.36 47.33 -8.72
Eisosomes 5.14 4.19 7.51 9.32 11.08 -4.76 -2.96 18.96 17.54 11.45 -1.91 -1.32 19.23 19.21 16.02
Endoplasmic Reticulum -4.92 -13.38 -13.07 -6.42 12.66 1.97 -9.35 -1.33 -2.84 8.83 1.68 -15.31 -2.31 -4.80 14.85
Endosome -3.22 2.95 3.26 7.57 1.60 -10.90 5.97 8.78 16.78 3.82 -11.42 7.09 9.05 18.20 3.99
Golgi -2.84 2.63 0.63 3.76 -2.40 -8.09 5.19 0.58 8.07 -2.94 -8.57 5.53 0.73 8.76 -3.76
Lipid Particles 2.72 2.89 4.80 7.04 8.80 1.57 4.82 12.06 10.09 9.15 2.73 6.20 12.72 11.35 12.79
Mitochondria -1.20 1.37 0.98 3.62 -2.45 -7.17 4.92 4.40 9.59 -2.89 -7.26 4.81 3.31 9.78 -2.91
Mitotic Spindle -3.52 -1.71 -7.16 -2.57 -1.31 -3.94 0.72 -6.56 -4.02 -6.93 -4.86 -1.46 -9.08 -5.06 -3.55
None -1.51 -2.94 -4.65 -0.16 -2.42 0.14 -0.13 -2.24 -1.82 -2.02 -0.36 -0.22 -3.04 -1.70 -3.00
Nuclear Periphery -1.66 -18.34 -10.33 -10.63 13.15 0.42 -7.99 -1.99 -2.34 9.33 0.52 -11.35 -2.68 -3.58 15.71
Nuclear Periphery Foci -2.67 -10.10 -11.52 -0.44 8.44 -0.91 -9.43 -4.53 -2.82 7.60 -2.06 -13.62 -8.01 -3.15 11.28
Nucleolus -3.19 -0.48 0.55 3.25 0.94 -1.13 2.06 3.42 3.51 4.24 -1.28 2.05 3.02 3.88 3.17
Nucleus 2.46 -17.54 1.04 -2.46 20.13 20.12 -12.40 4.97 -14.84 13.63 18.86 -21.10 7.19 -14.10 23.67
Peroxisomes -5.74 4.07 3.84 7.51 -1.21 -11.41 11.25 10.65 14.41 -0.43 -12.78 11.93 11.05 15.94 -1.18
Vacuole -6.29 -10.64 -28.77 -20.69 -4.96 -7.50 -10.97 -27.74 -21.36 2.37 -9.29 -14.99 -40.52 -31.28 -1.24
Vacuole Periphery -3.07 2.81 -1.91 0.60 -3.34 -7.37 7.77 -0.75 3.73 -2.57 -7.98 7.92 -1.97 3.34 -4.17
Standard name
Human Ortholog
Description Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Rkr1p-Tae2p-Cdc48p-Npl4p-Ufd1p) is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Rkr1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC
Localization
Cell Percentages cytoplasm (13%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rqc1

Rqc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rqc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available