Standard name
Human Ortholog
Description Protein with a potential role in cell survival pathways; required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 – 0.99 0.99 0.86 0.97 0.92 0.82 0.85 0.87 0.84 0.83 0.98 0.94 0.93 0.98 0.96 0.97 0.95 0.86 0.94 0.91 0.93 0.77
Endoplasmic Reticulum 0 – 0 0 0 0 0.06 0.09 0.07 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.05
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0.14 0 0 0.07 0 0 0 0 0 0.13 0.15 0 0 0 0 0.05 0 0 0 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 1 2 7 6 6 8 0 0 0 0 0 0 0 0 0 0 0 3
Bud 2 – 0 1 0 0 2 2 2 0 1 2 0 0 0 0 2 0 1 0 0 0 0 2
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 – 2 0 3 1 3 8 7 10 4 6 2 0 0 4 0 0 0 0 0 0 0 0
Cytoplasm 249 – 170 149 197 218 287 375 225 256 191 239 173 289 272 169 157 107 234 42 154 81 206 243
Endoplasmic Reticulum 3 – 1 0 4 3 18 43 18 15 10 13 1 3 3 5 10 3 0 0 2 2 1 12
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 2 0 0 1 2 1 5 1 1 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2 – 0 0 0 0 1 5 7 13 9 19 1 1 0 0 0 0 0 0 0 0 0 0
Nucleus 0 – 1 2 2 1 0 2 4 5 1 6 0 1 1 0 3 2 4 2 1 1 5 17
Nuclear Periphery 0 – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 0 0 0 0 2
Nucleolus 1 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 – 2 0 32 4 7 34 2 6 7 7 7 39 43 5 1 3 2 2 2 0 1 22
Unique Cell Count 254 172 150 230 224 312 457 264 295 227 289 177 308 292 173 164 110 248 49 165 89 222 315
Labelled Cell Count 261 176 152 240 227 319 473 274 313 234 301 185 333 319 183 174 115 248 49 165 89 222 315


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 29.9 – 29.1 29.4 32.1 25.8 26.5 23.8 22.7 21.4 20.8 21.0 34.7 33.6 32.3 39.1 44.4 53.4 29.9 29.6 31.6
Std Deviation (1e-4) 6.1 – 6.4 5.9 13.0 5.8 6.4 5.7 4.6 5.1 5.0 5.4 8.3 7.0 7.4 11.9 15.2 14.4 8.8 7.0 8.5
Intensity Change (Log2) – – – 0.02 0.14 -0.18 -0.14 -0.29 -0.36 -0.44 -0.49 -0.47 0.25 0.21 0.15 0.43 0.61 0.88 0.04 0.02 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 -4.6 -1.1 -3.1 -5.5 -4.8 -4.4 -5.0 -5.3 -0.8 -2.6 -2.8 -0.8 -1.8 -1.0
Endoplasmic Reticulum 0 0 0 2.8 3.9 3.1 2.6 0 2.4 0 0 0 0 2.8 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 4.5 0 0 3.0 0 0 0 0 0 4.3 4.8 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 28.0596 36.9571 32.4041 32.8304 32.5101 32.5163 25.4666 34.0646 29.8178 31.9555 26.9186 31.1858 25.0204 36.4045 30.2296 34.3848 31.5896 31.7586
Actin 0.0168 0.0001 0.0125 0.0014 0.0005 0.0007 0.0167 0 0.0067 0.0001 0.0043 0.0012 0.0337 0.0001 0.0229 0 0.001 0.0021
Bud 0.0009 0 0.0004 0.0012 0.0004 0.0006 0.0012 0 0.0007 0 0.0004 0.0002 0.0011 0 0.0009 0 0.0001 0.0002
Bud Neck 0.0016 0.0002 0.0012 0.0005 0.0006 0.0016 0.0007 0.0001 0.0003 0.0001 0.0003 0.0004 0.0019 0.0001 0.0007 0.0002 0.0008 0.0017
Bud Periphery 0.0009 0 0.0004 0.0014 0.0005 0.0005 0.0005 0 0.0003 0 0.0001 0.0001 0.0008 0 0.0009 0 0 0.0004
Bud Site 0.0049 0 0.0002 0.0014 0.0003 0.0002 0.0009 0 0.0032 0 0.0005 0.0002 0.0031 0 0.001 0 0.0001 0.0001
Cell Periphery 0.0005 0 0.0001 0.0002 0.0002 0.0002 0.0001 0 0.0001 0 0 0 0.0003 0 0.0002 0 0 0.0001
Cytoplasm 0.7282 0.9579 0.8762 0.8536 0.8389 0.8333 0.8405 0.9828 0.9246 0.9781 0.91 0.9513 0.7306 0.9559 0.8471 0.9831 0.8848 0.8648
Cytoplasmic Foci 0.0128 0.0001 0.0039 0.0034 0.0016 0.0096 0.0201 0.0001 0.0195 0.0001 0.0165 0.0021 0.0263 0.0001 0.0128 0.0001 0.0042 0.0015
Eisosomes 0.0002 0 0 0.0001 0 0 0.0001 0 0 0 0 0 0.0004 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0073 0.0003 0.0017 0.0015 0.0012 0.0019 0.0025 0.0001 0.0005 0.0001 0.0007 0.0012 0.0057 0.0002 0.0011 0.0001 0.0005 0.0033
Endosome 0.0092 0.0001 0.0013 0.0018 0.0024 0.0021 0.0066 0 0.0042 0.0001 0.0067 0.0024 0.018 0.0001 0.0115 0 0.0021 0.0023
Golgi 0.0021 0 0.0008 0.0003 0.0005 0.0003 0.0022 0 0.0029 0 0.0079 0.0008 0.0079 0 0.0063 0 0.0023 0.0018
Lipid Particles 0.0039 0 0.0038 0.0014 0.0052 0.0003 0.0113 0 0.0019 0 0.0016 0.0003 0.0259 0 0.0091 0 0.0551 0.0122
Mitochondria 0.0104 0.0001 0.0054 0.0147 0.0143 0.0146 0.0022 0 0.0045 0 0.0012 0.0038 0.0222 0.0001 0.0351 0 0.0022 0.0023
None 0.0048 0.0001 0.0007 0.0034 0.0004 0.0025 0.0042 0.0001 0.0015 0.0001 0.0011 0.0003 0.0096 0.0001 0.0016 0.0001 0.0003 0.0003
Nuclear Periphery 0.0283 0.0052 0.0135 0.016 0.0344 0.0182 0.0134 0.0022 0.0021 0.0034 0.0066 0.0036 0.0196 0.0038 0.0044 0.0013 0.0044 0.0121
Nucleolus 0.0015 0 0.0003 0.0016 0.001 0.0006 0.0005 0 0.0001 0 0 0 0.0041 0 0.0021 0 0.0002 0.0001
Nucleus 0.1473 0.0355 0.0719 0.0903 0.0893 0.1065 0.0642 0.0144 0.0231 0.0176 0.0297 0.0307 0.0629 0.0383 0.0308 0.0148 0.0239 0.0877
Peroxisomes 0.002 0 0.0012 0.0014 0.0008 0.0003 0.0022 0 0.0026 0 0.0095 0.0002 0.0075 0 0.0031 0 0.0156 0.0017
Punctate Nuclear 0.0058 0.0001 0.0012 0.0012 0.0005 0.0013 0.0077 0 0.0009 0.0001 0.0007 0.0003 0.012 0.0001 0.001 0.0001 0.0018 0.0016
Vacuole 0.0085 0.0003 0.0022 0.002 0.0047 0.0034 0.0016 0.0001 0.0003 0.0001 0.0018 0.0004 0.0038 0.0008 0.0053 0.0001 0.0003 0.0022
Vacuole Periphery 0.0021 0.0001 0.0011 0.0013 0.0024 0.0013 0.0006 0 0.0002 0.0001 0.0003 0.0002 0.0027 0.0002 0.0022 0.0001 0.0002 0.0015

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 137.7348 135.064 116.421 86.517 99.4305 106.7712 132.5972 122.6617 97.9258 94.363
Translational Efficiency 1.2114 1.4075 1.0902 1.2044 0.9777 1.2044 1.0477 0.8578 0.8104 0.9162

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1868 944 194 145 123 1461 174 1118 1991 2405 368 1263

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1763.72 1888.43 3210.06 3642.50 2625.54 2275.03 3522.73 3789.38 1816.96 2123.28 3357.90 3772.52
Standard Deviation 646.01 643.05 457.52 757.28 431.15 463.42 577.88 823.84 667.89 573.07 540.94 817.82
Intensity Change Log 2 — 0.098566 0.863979 1.046307 — -0.206729 0.424080 0.529348 — -0.076195 0.617227 0.759749

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000272 0.000342 0.000412 0.001741 0.000950 0.000304 0.000214 0.001325 0.000314 0.000319 0.000318 0.001373
Bud Neck 0.002196 0.001096 0.005174 0.005020 0.002715 0.000964 0.002342 0.013335 0.002228 0.001015 0.003835 0.012381
Bud Site 0.016366 0.003104 0.002322 0.027125 0.002431 0.002582 0.002460 0.012483 0.015505 0.002787 0.002387 0.014164
Cell Periphery 0.000369 0.000499 0.001576 0.000506 0.000943 0.000218 0.000583 0.000622 0.000404 0.000329 0.001106 0.000609
Cytoplasm 0.816437* 0.664846* 0.358407* 0.373087* 0.477359* 0.648246* 0.327351* 0.364698* 0.795490* 0.654762* 0.343723* 0.365661*
Cytoplasmic Foci 0.036515 0.005110 0.023739 0.009224 0.013727 0.005893 0.009233 0.019386 0.035107 0.005586 0.016880 0.018219
Eisosomes 0.000002 0.000002 0.000006 0.000006 0.000004 0.000003 0.000003 0.000010 0.000002 0.000002 0.000004 0.000010
Endoplasmic Reticulum 0.000523 0.000217 0.000125 0.000327 0.000428 0.000306 0.000061 0.000530 0.000517 0.000271 0.000094 0.000506
Endosome 0.002252 0.000265 0.000190 0.003383 0.000314 0.000646 0.000068 0.013023 0.002132 0.000497 0.000132 0.011916
Golgi 0.008068 0.001398 0.001018 0.003867 0.001789 0.001747 0.000177 0.008737 0.007680 0.001610 0.000620 0.008178
Lipid Particles 0.000908 0.000456 0.001803 0.001007 0.001123 0.000403 0.000862 0.008222 0.000921 0.000424 0.001358 0.007393
Mitochondria 0.000668 0.000235 0.000608 0.000858 0.000438 0.000721 0.000371 0.002472 0.000653 0.000530 0.000496 0.002287
Mitotic Spindle 0.029894 0.001742 0.001338 0.011300 0.000373 0.002803 0.014563 0.083012 0.028070 0.002386 0.007591 0.074779
None 0.005132 0.032630 0.006312 0.012401 0.004269 0.010981 0.013576 0.024466 0.005078 0.019479 0.009747 0.023080
Nuclear Periphery 0.000181 0.000118 0.000920 0.001395 0.000249 0.000126 0.000471 0.002010 0.000185 0.000123 0.000708 0.001939
Nuclear Periphery Foci 0.002666 0.002369 0.001636 0.012357 0.003709 0.001804 0.000531 0.004381 0.002731 0.002026 0.001114 0.005297
Nucleolus 0.000325 0.000444 0.000197 0.000118 0.000145 0.001137 0.000092 0.001113 0.000314 0.000865 0.000147 0.000998
Nucleus 0.053896 0.278724* 0.578708* 0.514473* 0.479205* 0.313281* 0.619745* 0.401716* 0.080171 0.299717* 0.598112* 0.414661*
Peroxisomes 0.000479 0.000039 0.000031 0.000542 0.000031 0.000125 0.000019 0.000580 0.000452 0.000091 0.000025 0.000576
Vacuole 0.015276 0.006122 0.015217 0.019774 0.009588 0.006019 0.007228 0.027767 0.014925 0.006059 0.011440 0.026850
Vacuole Periphery 0.007576 0.000239 0.000263 0.001488 0.000211 0.001691 0.000051 0.010112 0.007121 0.001121 0.000163 0.009122

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.09 -5.99 -1.36 -1.12 -0.15 4.27 4.09 3.71 -0.68 -0.44 -0.71 -4.34 -1.37 -1.09 1.24
Bud Neck 3.64 -6.68 -3.94 -4.72 2.45 4.05 -2.66 -3.65 -8.79 0.41 6.55 -7.37 -7.05 -9.48 2.70
Bud Site 10.43 8.10 0.73 -1.68 -1.39 0.63 -1.37 -3.33 -4.19 -1.11 11.12 8.69 6.71 -4.00 -1.67
Cell Periphery -1.86 -9.73 -4.55 -3.46 6.34 4.83 -1.06 2.42 -9.22 3.81 0.98 -10.42 -5.39 -7.67 8.62
Cytoplasm 17.88 35.43 25.52 16.16 -1.80 -7.70 7.17 3.91 30.09 -6.44 20.51 46.22 51.41 35.55 -7.19
Cytoplasmic Foci 14.59 -1.05 2.82 -4.58 2.95 2.54 -0.97 0.60 -6.95 2.55 15.55 0.90 9.39 -8.16 5.80
Eisosomes -0.47 -7.38 -4.55 -4.38 2.28 2.26 -2.68 -2.75 -6.14 1.39 -0.41 -8.35 -11.25 -8.53 3.60
Endoplasmic Reticulum 3.33 3.64 3.85 1.32 1.04 2.38 4.52 4.60 2.49 -0.20 3.13 4.39 4.78 2.99 1.06
Endosome 11.05 10.79 2.68 -0.83 -0.70 -1.10 2.29 -2.56 -2.20 -2.94 9.82 11.51 1.52 -2.47 -2.83
Golgi 7.16 7.48 6.33 0.28 0.65 0.83 4.33 1.40 0.59 -1.95 7.35 8.38 7.30 0.47 -0.60
Lipid Particles 1.68 -5.93 -2.42 -2.73 1.04 3.10 -1.82 -2.29 -4.43 -0.43 3.13 -6.32 -3.93 -4.87 1.10
Mitochondria 6.54 -1.74 1.29 -2.44 2.50 -0.67 -2.16 -1.70 -0.08 -0.05 0.99 -1.17 0.12 -0.84 0.94
Mitotic Spindle 10.99* 11.72 1.03 -1.87 -1.95 -3.60 -1.96 -6.05 -5.07 -0.90 11.52 6.01 2.87 -5.40 -2.84
None -8.13 -1.58 -4.52 5.67 -3.92 -9.76 -6.21 -8.23 -2.39 -2.10 -10.17 -4.88 -7.78 2.70 -4.64
Nuclear Periphery 1.42 -11.74 -3.27 -3.39 0.81 4.66 -7.36 -8.61 -10.20 0.47 3.02 -13.78 -9.92 -10.61 2.03
Nuclear Periphery Foci -0.49 -0.90 -0.61 -0.48 -0.38 3.43 4.61 3.95 0.92 -2.04 1.13 1.48 2.02 1.16 0.78
Nucleolus -0.98 1.98 2.80 1.57 1.11 -1.71 -1.02 -2.86 1.30 -2.46 -1.55 3.62 0.30 1.59 -1.63
Nucleus -31.10* -33.19 -19.56 -8.65 2.59 6.80 -4.36 0.66 -15.31 7.80 -40.08* -43.38 -49.53 -19.02 8.69
Peroxisomes 3.72 3.50 2.29 -0.97 -0.70 -1.51 -0.20 -1.77 0.40 -1.61 3.27 3.60 3.18 -0.29 -1.40
Vacuole 4.64 -4.61 -4.69 -5.47 -1.81 1.89 -4.38 -14.66 -17.30 -4.37 6.87 -5.72 -14.88 -17.96 -5.50
Vacuole Periphery 5.86 5.72 5.59 -0.56 0.04 -2.37 2.78 -1.93 0.14 -2.13 5.03 5.82 4.55 -0.45 -1.90
Standard name
Human Ortholog
Description Protein with a potential role in cell survival pathways; required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance
Localization
Cell Percentages cytoplasm (95%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Svf1

Svf1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Svf1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available