Standard name | |
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Human Ortholog | |
Description | Protein with a potential role in cell survival pathways; required for the diauxic growth shift; expression in mammalian cells increases survival under conditions inducing apoptosis; mutant has increased aneuploidy tolerance |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | 0 | 0 | 1 | 2 | 7 | 6 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Bud | 2 | – | 0 | 1 | 0 | 0 | 2 | 2 | 2 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | – | 2 | 0 | 3 | 1 | 3 | 8 | 7 | 10 | 4 | 6 | 2 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 249 | – | 170 | 149 | 197 | 218 | 287 | 375 | 225 | 256 | 191 | 239 | 173 | 289 | 272 | 169 | 157 | 107 | 234 | 42 | 154 | 81 | 206 | 243 |
Endoplasmic Reticulum | 3 | – | 1 | 0 | 4 | 3 | 18 | 43 | 18 | 15 | 10 | 13 | 1 | 3 | 3 | 5 | 10 | 3 | 0 | 0 | 2 | 2 | 1 | 12 |
Endosome | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | – | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 1 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | – | 0 | 0 | 0 | 0 | 1 | 5 | 7 | 13 | 9 | 19 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | – | 1 | 2 | 2 | 1 | 0 | 2 | 4 | 5 | 1 | 6 | 0 | 1 | 1 | 0 | 3 | 2 | 4 | 2 | 1 | 1 | 5 | 17 |
Nuclear Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 |
Nucleolus | 1 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 1 | – | 2 | 0 | 32 | 4 | 7 | 34 | 2 | 6 | 7 | 7 | 7 | 39 | 43 | 5 | 1 | 3 | 2 | 2 | 2 | 0 | 1 | 22 |
Unique Cell Count | 254 | 172 | 150 | 230 | 224 | 312 | 457 | 264 | 295 | 227 | 289 | 177 | 308 | 292 | 173 | 164 | 110 | 248 | 49 | 165 | 89 | 222 | 315 | |
Labelled Cell Count | 261 | 176 | 152 | 240 | 227 | 319 | 473 | 274 | 313 | 234 | 301 | 185 | 333 | 319 | 183 | 174 | 115 | 248 | 49 | 165 | 89 | 222 | 315 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 29.9 | – | 29.1 | 29.4 | 32.1 | 25.8 | 26.5 | 23.8 | 22.7 | 21.4 | 20.8 | 21.0 | 34.7 | 33.6 | 32.3 | 39.1 | 44.4 | 53.4 | 29.9 | 29.6 | 31.6 |
Std Deviation (1e-4) | 6.1 | – | 6.4 | 5.9 | 13.0 | 5.8 | 6.4 | 5.7 | 4.6 | 5.1 | 5.0 | 5.4 | 8.3 | 7.0 | 7.4 | 11.9 | 15.2 | 14.4 | 8.8 | 7.0 | 8.5 |
Intensity Change (Log2) | – | – | – | 0.02 | 0.14 | -0.18 | -0.14 | -0.29 | -0.36 | -0.44 | -0.49 | -0.47 | 0.25 | 0.21 | 0.15 | 0.43 | 0.61 | 0.88 | 0.04 | 0.02 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | -4.6 | -1.1 | -3.1 | -5.5 | -4.8 | -4.4 | -5.0 | -5.3 | -0.8 | -2.6 | -2.8 | -0.8 | -1.8 | -1.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 2.8 | 3.9 | 3.1 | 2.6 | 0 | 2.4 | 0 | 0 | 0 | 0 | 2.8 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 4.5 | 0 | 0 | 3.0 | 0 | 0 | 0 | 0 | 0 | 4.3 | 4.8 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 28.0596 | 36.9571 | 32.4041 | 32.8304 | 32.5101 | 32.5163 | 25.4666 | 34.0646 | 29.8178 | 31.9555 | 26.9186 | 31.1858 | 25.0204 | 36.4045 | 30.2296 | 34.3848 | 31.5896 | 31.7586 |
Actin | 0.0168 | 0.0001 | 0.0125 | 0.0014 | 0.0005 | 0.0007 | 0.0167 | 0 | 0.0067 | 0.0001 | 0.0043 | 0.0012 | 0.0337 | 0.0001 | 0.0229 | 0 | 0.001 | 0.0021 |
Bud | 0.0009 | 0 | 0.0004 | 0.0012 | 0.0004 | 0.0006 | 0.0012 | 0 | 0.0007 | 0 | 0.0004 | 0.0002 | 0.0011 | 0 | 0.0009 | 0 | 0.0001 | 0.0002 |
Bud Neck | 0.0016 | 0.0002 | 0.0012 | 0.0005 | 0.0006 | 0.0016 | 0.0007 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0004 | 0.0019 | 0.0001 | 0.0007 | 0.0002 | 0.0008 | 0.0017 |
Bud Periphery | 0.0009 | 0 | 0.0004 | 0.0014 | 0.0005 | 0.0005 | 0.0005 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0008 | 0 | 0.0009 | 0 | 0 | 0.0004 |
Bud Site | 0.0049 | 0 | 0.0002 | 0.0014 | 0.0003 | 0.0002 | 0.0009 | 0 | 0.0032 | 0 | 0.0005 | 0.0002 | 0.0031 | 0 | 0.001 | 0 | 0.0001 | 0.0001 |
Cell Periphery | 0.0005 | 0 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0.0001 |
Cytoplasm | 0.7282 | 0.9579 | 0.8762 | 0.8536 | 0.8389 | 0.8333 | 0.8405 | 0.9828 | 0.9246 | 0.9781 | 0.91 | 0.9513 | 0.7306 | 0.9559 | 0.8471 | 0.9831 | 0.8848 | 0.8648 |
Cytoplasmic Foci | 0.0128 | 0.0001 | 0.0039 | 0.0034 | 0.0016 | 0.0096 | 0.0201 | 0.0001 | 0.0195 | 0.0001 | 0.0165 | 0.0021 | 0.0263 | 0.0001 | 0.0128 | 0.0001 | 0.0042 | 0.0015 |
Eisosomes | 0.0002 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0073 | 0.0003 | 0.0017 | 0.0015 | 0.0012 | 0.0019 | 0.0025 | 0.0001 | 0.0005 | 0.0001 | 0.0007 | 0.0012 | 0.0057 | 0.0002 | 0.0011 | 0.0001 | 0.0005 | 0.0033 |
Endosome | 0.0092 | 0.0001 | 0.0013 | 0.0018 | 0.0024 | 0.0021 | 0.0066 | 0 | 0.0042 | 0.0001 | 0.0067 | 0.0024 | 0.018 | 0.0001 | 0.0115 | 0 | 0.0021 | 0.0023 |
Golgi | 0.0021 | 0 | 0.0008 | 0.0003 | 0.0005 | 0.0003 | 0.0022 | 0 | 0.0029 | 0 | 0.0079 | 0.0008 | 0.0079 | 0 | 0.0063 | 0 | 0.0023 | 0.0018 |
Lipid Particles | 0.0039 | 0 | 0.0038 | 0.0014 | 0.0052 | 0.0003 | 0.0113 | 0 | 0.0019 | 0 | 0.0016 | 0.0003 | 0.0259 | 0 | 0.0091 | 0 | 0.0551 | 0.0122 |
Mitochondria | 0.0104 | 0.0001 | 0.0054 | 0.0147 | 0.0143 | 0.0146 | 0.0022 | 0 | 0.0045 | 0 | 0.0012 | 0.0038 | 0.0222 | 0.0001 | 0.0351 | 0 | 0.0022 | 0.0023 |
None | 0.0048 | 0.0001 | 0.0007 | 0.0034 | 0.0004 | 0.0025 | 0.0042 | 0.0001 | 0.0015 | 0.0001 | 0.0011 | 0.0003 | 0.0096 | 0.0001 | 0.0016 | 0.0001 | 0.0003 | 0.0003 |
Nuclear Periphery | 0.0283 | 0.0052 | 0.0135 | 0.016 | 0.0344 | 0.0182 | 0.0134 | 0.0022 | 0.0021 | 0.0034 | 0.0066 | 0.0036 | 0.0196 | 0.0038 | 0.0044 | 0.0013 | 0.0044 | 0.0121 |
Nucleolus | 0.0015 | 0 | 0.0003 | 0.0016 | 0.001 | 0.0006 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0.0041 | 0 | 0.0021 | 0 | 0.0002 | 0.0001 |
Nucleus | 0.1473 | 0.0355 | 0.0719 | 0.0903 | 0.0893 | 0.1065 | 0.0642 | 0.0144 | 0.0231 | 0.0176 | 0.0297 | 0.0307 | 0.0629 | 0.0383 | 0.0308 | 0.0148 | 0.0239 | 0.0877 |
Peroxisomes | 0.002 | 0 | 0.0012 | 0.0014 | 0.0008 | 0.0003 | 0.0022 | 0 | 0.0026 | 0 | 0.0095 | 0.0002 | 0.0075 | 0 | 0.0031 | 0 | 0.0156 | 0.0017 |
Punctate Nuclear | 0.0058 | 0.0001 | 0.0012 | 0.0012 | 0.0005 | 0.0013 | 0.0077 | 0 | 0.0009 | 0.0001 | 0.0007 | 0.0003 | 0.012 | 0.0001 | 0.001 | 0.0001 | 0.0018 | 0.0016 |
Vacuole | 0.0085 | 0.0003 | 0.0022 | 0.002 | 0.0047 | 0.0034 | 0.0016 | 0.0001 | 0.0003 | 0.0001 | 0.0018 | 0.0004 | 0.0038 | 0.0008 | 0.0053 | 0.0001 | 0.0003 | 0.0022 |
Vacuole Periphery | 0.0021 | 0.0001 | 0.0011 | 0.0013 | 0.0024 | 0.0013 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0002 | 0.0027 | 0.0002 | 0.0022 | 0.0001 | 0.0002 | 0.0015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 137.7348 | 135.064 | 116.421 | 86.517 | 99.4305 | 106.7712 | 132.5972 | 122.6617 | 97.9258 | 94.363 |
Translational Efficiency | 1.2114 | 1.4075 | 1.0902 | 1.2044 | 0.9777 | 1.2044 | 1.0477 | 0.8578 | 0.8104 | 0.9162 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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