Standard name
Human Ortholog
Description Anthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway; catalyzes the phosphoribosylation of anthranilate; subject to the general control system of amino acid biosynthesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 – 0 0 0 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – 0.06 – 0.06 0 0 0.05 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 – – 1.0 – 0.98 0.98 0.88 0.89 – 0.75 1.0 0.98 0.98 0.99 0.95 1.0 0.97 0.97 0.94 0.94 0.95 0.94
Endoplasmic Reticulum 0 0 – – 0 – 0 0 0 0 – 0.06 0 0 0 0.08 0 0 0 0 0 0 0 0
Endosome 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – – 0 – 0 0 0.06 0.05 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – 0 – 0 0 0.06 0 – 0.12 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 – 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – 0 – 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 1 2 3
Bud Neck 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – 1 – 3 0 0 1 – 1 5 10 5 2 1 0 0 0 0 0 0 0
Cytoplasm 55 112 – – 18 – 48 44 14 17 – 12 399 729 727 92 62 45 74 105 16 186 396 456
Endoplasmic Reticulum 0 0 – – 0 – 0 0 0 0 – 1 1 3 0 7 2 2 0 0 0 0 1 1
Endosome 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 2
Golgi 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – – 0 – 0 0 1 1 – 0 0 0 0 0 0 0 0 0 0 2 0 1
Nucleus 0 0 – – 0 – 0 1 0 0 – 0 5 6 0 0 2 0 0 0 0 2 6 3
Nuclear Periphery 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 1
Nucleolus 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 – 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 0 0 – – 0 – 0 0 1 0 – 2 0 11 18 1 0 0 0 0 0 3 3 5
Unique Cell Count 55 112 18 49 45 16 19 16 400 742 744 93 65 45 77 109 18 198 419 485
Labelled Cell Count 55 112 19 51 45 16 20 16 411 759 750 102 68 47 77 109 18 198 419 485


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.1 32.6 – – 21.6 – 20.1 19.8 16.7 17.2 – 15.5 27.5 27.1 25.7 38.8 37.4 39.2 25.1 25.3 27.1
Std Deviation (1e-4) 5.5 5.9 – – 3.0 – 4.3 4.0 4.8 3.7 – 5.3 5.0 5.2 5.8 12.7 14.5 11.2 5.2 5.7 6.3
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 25.5231 33.8452 29.9465 29.9474 26.4724 31.8805 28.1153 39.3527 33.9147 30.3284 33.5532 35.9864 27.4932 37.023 31.2604 29.6334 29.5553 31.7471
Actin 0.0266 0.0001 0.0055 0.0001 0.001 0.0056 0.0207 0.0001 0.013 0.0174 0.0036 0.0012 0.0058 0 0.004 0.0347 0.0001 0.0044
Bud 0.0005 0.0001 0.0002 0.0001 0.0005 0.0001 0.0005 0.0005 0.0005 0.0013 0.0001 0 0.0017 0 0.0002 0.0018 0.0004 0.0001
Bud Neck 0.0008 0.0002 0.0004 0.0002 0.0005 0.0006 0.0014 0.0001 0.0004 0.0004 0.0002 0.0005 0.0008 0.0001 0.0003 0.0007 0.0008 0.0004
Bud Periphery 0.0003 0.0001 0.0003 0.0001 0.0004 0 0.0005 0.0002 0.001 0.0017 0.0001 0 0.001 0 0.0002 0.0019 0.0001 0.0002
Bud Site 0.0009 0.0001 0.0006 0 0.0006 0 0.0031 0 0.0013 0.0019 0 0 0.0009 0 0.0032 0.0006 0.0001 0.0001
Cell Periphery 0.0001 0 0.0002 0 0.0003 0 0.0002 0 0.0001 0.0001 0.0001 0 0.0002 0 0.0001 0.0002 0 0
Cytoplasm 0.8855 0.9879 0.9608 0.981 0.887 0.9712 0.8538 0.9923 0.9544 0.891 0.9444 0.9761 0.9022 0.9977 0.9513 0.8765 0.931 0.9675
Cytoplasmic Foci 0.0117 0.0002 0.0027 0.0009 0.0323 0.0006 0.016 0.0003 0.0044 0.0184 0.0002 0.0007 0.012 0.0001 0.0053 0.0046 0.001 0.0009
Eisosomes 0.0001 0 0.0002 0 0.0001 0.0001 0.0002 0 0.0001 0.0003 0.0008 0 0.0001 0 0 0.0002 0 0.0001
Endoplasmic Reticulum 0.0029 0.0002 0.0045 0.0016 0.0067 0.0012 0.0044 0.0001 0.0007 0.0022 0.0004 0.0008 0.0039 0.0001 0.0016 0.0007 0.0013 0.0005
Endosome 0.0183 0.0016 0.0035 0.0024 0.0114 0.001 0.0154 0.0005 0.0022 0.0153 0.0011 0.0016 0.0115 0 0.0046 0.0083 0.0055 0.0024
Golgi 0.0046 0.0001 0.0007 0.0001 0.001 0.0003 0.0067 0 0.0016 0.0076 0.0028 0.0012 0.0044 0 0.0015 0.013 0.0001 0.0027
Lipid Particles 0.003 0 0.0011 0.0001 0.0076 0.0003 0.0182 0 0.0027 0.0085 0.0007 0.0069 0.0046 0 0.0032 0.0231 0 0.0029
Mitochondria 0.0017 0.0002 0.0011 0.0001 0.0041 0.0001 0.0046 0.0003 0.0061 0.0098 0.0238 0.0006 0.0049 0 0.003 0.0083 0.0004 0.0049
None 0.0029 0.0001 0.0015 0.0004 0.0081 0.003 0.0051 0.0002 0.001 0.0035 0.0003 0.0003 0.0031 0.0001 0.0015 0.0007 0.0002 0.0003
Nuclear Periphery 0.0075 0.0008 0.0049 0.0019 0.0081 0.0034 0.0087 0.0004 0.0011 0.0024 0.0016 0.0019 0.0108 0.0002 0.0029 0.0012 0.0239 0.0016
Nucleolus 0.0011 0.0003 0.0001 0 0.0016 0.0001 0.0018 0 0.0002 0.0004 0 0 0.0009 0 0.0007 0.0004 0.0001 0.0001
Nucleus 0.0195 0.005 0.0081 0.0075 0.0121 0.0105 0.0167 0.0024 0.0037 0.0089 0.0188 0.0062 0.019 0.0015 0.0058 0.0071 0.0189 0.009
Peroxisomes 0.0013 0 0.0001 0 0.001 0.0002 0.0058 0 0.0022 0.0031 0.0002 0.0011 0.0011 0 0.0017 0.0027 0 0.0009
Punctate Nuclear 0.0051 0.0001 0.0014 0.0001 0.0056 0.0011 0.0091 0 0.0018 0.0029 0.0001 0.0006 0.0053 0 0.0008 0.0002 0.0002 0.0003
Vacuole 0.0043 0.0026 0.0015 0.003 0.0071 0.0005 0.0052 0.0016 0.0007 0.0018 0.0002 0.0002 0.004 0.0002 0.0057 0.0082 0.0111 0.0003
Vacuole Periphery 0.0014 0.0004 0.0006 0.0004 0.003 0.0001 0.0021 0.0007 0.0006 0.0011 0.0003 0.0002 0.0016 0 0.0021 0.0049 0.0049 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 49.2333 38.8337 30.7374 48.308 47.5619 43.4171 44.7182 57.7134 51.7981 54.4027
Translational Efficiency 1.0435 1.1678 1.383 0.9991 1.0006 1.3041 1.25 0.9187 1.1465 1.0318

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
940 213 1752 668 1856 455 553 1148 2796 668 2305 1816

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2105.84 2800.02 1887.65 1200.37 1996.91 2377.02 1618.70 1125.00 2033.53 2511.90 1823.13 1152.72
Standard Deviation 471.02 529.09 310.58 287.51 376.78 451.61 272.43 237.96 414.09 516.76 322.98 259.85
Intensity Change Log 2 — 0.411041 -0.157805 -0.810917 — 0.251385 -0.302934 -0.827844 — 0.335536 -0.226621 -0.819131

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000276 0.000329 0.000161 0.001065 0.000394 0.000413 0.000424 0.000738 0.000354 0.000386 0.000224 0.000859
Bud Neck 0.000623 0.001341 0.001007 0.002694 0.000268 0.001355 0.002143 0.003083 0.000387 0.001351 0.001280 0.002940
Bud Site 0.002650 0.005859 0.002070 0.026731 0.001402 0.004394 0.008629 0.024498 0.001821 0.004861 0.003643 0.025319
Cell Periphery 0.000354 0.000494 0.000129 0.000453 0.000142 0.000342 0.000140 0.000081 0.000213 0.000391 0.000132 0.000218
Cytoplasm 0.895376* 0.800173* 0.802685* 0.726933* 0.905162* 0.850930* 0.784357* 0.622092* 0.901872* 0.834746* 0.798288* 0.660657*
Cytoplasmic Foci 0.010650 0.016019 0.007198 0.021761 0.004616 0.016984 0.012837 0.023449 0.006644 0.016676 0.008551 0.022828
Eisosomes 0.000001 0.000005 0.000002 0.000030 0.000001 0.000002 0.000005 0.000021 0.000001 0.000003 0.000002 0.000024
Endoplasmic Reticulum 0.000390 0.000320 0.000131 0.002779 0.000759 0.000176 0.000884 0.000583 0.000635 0.000222 0.000311 0.001390
Endosome 0.000428 0.000797 0.000414 0.003663 0.000256 0.001083 0.001377 0.014164 0.000314 0.000992 0.000645 0.010301
Golgi 0.002672 0.001549 0.000324 0.002343 0.000950 0.003356 0.000381 0.008287 0.001529 0.002780 0.000338 0.006100
Lipid Particles 0.001390 0.003015 0.000650 0.001069 0.000589 0.001077 0.001288 0.000801 0.000858 0.001695 0.000803 0.000899
Mitochondria 0.000066 0.001598 0.000156 0.004105 0.000121 0.000112 0.000128 0.006278 0.000103 0.000586 0.000149 0.005479
Mitotic Spindle 0.001206 0.001415 0.006647 0.028807 0.000627 0.007494 0.014890 0.074034 0.000822 0.005556 0.008625 0.057398
None 0.005800 0.008194 0.005723 0.003039 0.006338 0.005950 0.004668 0.008705 0.006157 0.006666 0.005470 0.006621
Nuclear Periphery 0.000074 0.000184 0.000259 0.000264 0.000056 0.000114 0.000421 0.000496 0.000062 0.000137 0.000298 0.000410
Nuclear Periphery Foci 0.004997 0.003708 0.001438 0.002206 0.003306 0.004320 0.004199 0.001362 0.003875 0.004125 0.002100 0.001672
Nucleolus 0.000060 0.000142 0.000127 0.000106 0.000065 0.000165 0.000215 0.000561 0.000063 0.000158 0.000148 0.000393
Nucleus 0.062501 0.141585* 0.153538* 0.070608 0.069683 0.089774 0.105076 0.064441 0.067268 0.106295 0.141911* 0.066710
Peroxisomes 0.000019 0.003138 0.000031 0.001299 0.000037 0.000037 0.000812 0.000768 0.000031 0.001026 0.000218 0.000963
Vacuole 0.009576 0.009638 0.017199 0.098291 0.004861 0.009345 0.056929 0.140518 0.006446 0.009438 0.026731 0.124985
Vacuole Periphery 0.000892 0.000495 0.000111 0.001755 0.000368 0.002576 0.000198 0.005040 0.000544 0.001913 0.000132 0.003832

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.21 1.30 -3.06 -1.44 -4.20 -0.18 -0.26 -0.03 0.28 0.44 -0.42 0.37 -0.68 -0.43 -2.30
Bud Neck -2.78 -8.15 -6.33 0.30 1.20 -5.55 -10.65 -12.27 -1.63 1.86 -5.90 -13.94 -13.81 -1.04 1.69
Bud Site -1.17 -1.37 -6.45 -3.02 -6.17 -4.01 -7.15 -7.56 -4.73 -2.24 -3.33 -7.17 -9.95 -5.67 -7.25
Cell Periphery -2.38 3.91 2.57 3.94 -0.03 -5.13 -1.86 5.57 6.98 7.81 -5.26 0.85 4.10 7.37 3.83
Cytoplasm 8.49 24.98 25.69 10.41 11.83 8.86 22.57 42.03 26.40 11.32 12.03 37.48 48.93 27.14 20.60
Cytoplasmic Foci -3.06 -0.18 -5.34 -0.36 -5.88 -6.60 -9.59 -10.76 2.08 1.50 -7.19 -9.02 -11.69 1.54 -4.13
Eisosomes -1.91 -5.80 -9.13 -5.90 -8.46 -3.41 -12.30 -22.92 -21.11 -12.33 -2.95 -13.69 -20.28 -15.79 -16.83
Endoplasmic Reticulum 0.35 1.68 -1.90 -2.01 -2.42 1.18 0.26 0.16 -9.09 -0.36 1.23 1.02 -0.68 -3.92 -3.56
Endosome -1.16 -3.25 -3.61 -1.48 -2.70 -2.33 -8.22 -4.58 -3.12 -2.82 -2.59 -8.19 -5.34 -3.40 -4.05
Golgi 1.76 4.63 3.78 2.88 -0.62 -2.64 6.86 -1.11 1.70 -2.36 -1.92 6.54 1.00 2.31 -2.37
Lipid Particles -1.36 0.65 0.07 1.37 -0.56 -3.23 -5.95 -3.64 1.91 4.65 -2.45 -5.19 -1.95 1.90 3.33
Mitochondria -1.05 -8.96 -1.33 0.25 -1.13 0.17 -0.42 -2.47 -2.50 -2.43 -1.03 -2.41 -2.75 -1.29 -2.54
Mitotic Spindle -0.49 -8.67 -4.27 -4.12 -1.47 -2.97 -5.78 -8.06 -5.06 -4.13 -2.99 -10.92 -9.12 -6.03 -5.04
None -1.43 0.53 3.77 4.73 9.04 0.85 5.49 0.82 -0.12 -3.40 -0.19 3.15 2.87 2.41 0.54
Nuclear Periphery -3.02 -18.85 -11.72 -5.15 -1.81 -5.32 -15.89 -22.00 -18.22 -4.33 -5.36 -24.98 -24.64 -17.06 -6.60
Nuclear Periphery Foci 0.41 6.37 5.87 2.99 -0.74 -2.68 -2.86 5.35 6.49 8.41 -1.90 3.51 7.60 6.89 5.79
Nucleolus -1.63 -11.13 -6.28 0.35 4.84 -2.22 -3.55 -3.56 -1.20 -0.97 -2.72 -8.12 -4.20 -0.92 -0.33
Nucleus -8.89 -24.32 -5.09 6.32 15.65 -5.70 -9.67 -6.19 0.71 4.34 -10.08 -27.25 -7.93 4.73 17.11
Peroxisomes -1.03 -6.97 -2.35 0.83 -2.16 -0.30 -1.45 -4.80 -4.95 0.09 -1.03 -1.87 -4.10 0.61 -2.68
Vacuole -1.62 -14.92 -23.76 -21.99 -18.68 -4.87 -16.82 -34.79 -33.19 -12.82 -4.49 -23.30 -42.05 -39.82 -28.25
Vacuole Periphery 0.99 2.20 1.11 0.09 -1.35 -1.12 1.56 -1.66 0.73 -2.41 -0.99 2.73 -0.90 0.76 -2.75
Standard name
Human Ortholog
Description Anthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway; catalyzes the phosphoribosylation of anthranilate; subject to the general control system of amino acid biosynthesis
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Trp4

Trp4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Trp4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available