Standard name
Human Ortholog
Description Phenylpyruvate decarboxylase; catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway; involved in protein N-terminal Met and Ala catabolism
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0.08 0.05 0 0.05 0.08
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.07 0.08 0 0 0 0 0 0
Cytoplasm 0.99 0.97 0.92 0.82 0.96 0.92 0.92 – 0.83 0.81 0.64 0.7 0.99 1.0 0.99 0.84 0.89 0.87 0.9 0.8 0.71 0.88 0.83 0.83
Endoplasmic Reticulum 0 0 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.13 0 0.05 0.06 – 0.28 0.26 0.51 0.32 0 0 0 0 0 0 0 0 0.06 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.08 0.11 0 0 0.06 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 – 0 0 0 1 0 0 0 1 0 0 0 3 1 0 0 3
Bud 0 2 0 1 0 1 1 – 2 5 2 2 0 0 0 0 0 1 4 20 4 3 8 19
Bud Neck 0 0 0 0 1 1 5 – 2 6 3 3 0 0 0 0 0 0 0 3 0 0 1 2
Bud Site 0 0 0 0 0 0 0 – 0 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 – 1 0 1 0 0 0 1 13 13 16 0 0 0 0 0 0
Cytoplasm 72 238 45 32 126 147 211 – 53 150 99 80 112 197 239 168 167 179 73 200 63 81 144 209
Endoplasmic Reticulum 0 2 4 0 1 1 1 – 1 0 1 3 0 0 1 11 5 10 0 1 1 0 0 0
Endosome 0 0 0 0 1 1 2 – 0 1 0 0 0 0 1 0 2 3 0 2 1 0 0 2
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 9 5 3 0 2 0 0 0 0
Mitochondria 1 1 0 5 1 8 13 – 18 49 79 37 0 0 0 4 1 1 1 4 5 0 2 2
Nucleus 0 1 1 1 1 2 2 – 1 6 0 1 0 1 0 0 0 1 0 2 1 0 3 1
Nuclear Periphery 0 0 0 0 0 0 1 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 0 0 0 2 0 0 3 – 1 0 0 0 0 0 0 7 4 6 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 1 – 0 1 3 4 0 0 0 1 1 0 0 4 1 0 2 2
Vac/Vac Membrane 0 3 0 1 5 3 2 – 0 4 0 1 1 0 1 15 15 23 0 2 5 1 1 1
Unique Cell Count 73 245 49 39 131 159 229 64 186 155 114 113 197 242 199 187 206 81 251 89 93 174 253
Labelled Cell Count 73 248 50 42 136 166 242 79 223 190 132 113 198 243 229 213 243 81 251 89 93 174 253


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.4 6.7 6.1 5.5 6.3 5.1 4.8 – 3.6 3.9 3.4 3.5 6.7 6.9 5.9 29.7 26.9 24.8 6.5 7.7 8.9
Std Deviation (1e-4) 3.7 2.8 2.0 1.5 2.3 1.7 1.7 – 1.2 1.5 1.9 1.2 2.5 4.0 1.5 22.5 16.8 18.4 2.9 8.0 12.7
Intensity Change (Log2) – – – -0.14 0.05 -0.26 -0.33 – -0.77 -0.63 -0.83 -0.78 0.14 0.17 -0.03 2.29 2.14 2.03 0.1 0.33 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 1.2 0.1 0.1 – -1.4 -1.9 -3.7 -3.0 2.5 4.0 2.9 -1.3 -0.5 -1.0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0505 7.7593 4.2624 2.4672 2.8457 0.9743 0.0257 4.4106 0.8886 -1.0699 -1.2409 1.4182 8.8852 16.4674 7.3194 8.1841 -0.6978 13.6611
Actin 0.0149 0.0013 0.0049 0.002 0.0026 0.0062 0.0134 0.0027 0.0099 0.0028 0.003 0.0087 0.0013 0.0004 0.0498 0.0015 0.0518 0.0008
Bud 0.0087 0.0191 0.0146 0.0148 0.0231 0.0079 0.004 0.0079 0.0179 0.0001 0.0143 0.0041 0.0119 0.0334 0.0081 0.0123 0.0005 0.0078
Bud Neck 0.001 0.0003 0.0006 0.0003 0.0012 0.0021 0.0004 0.0001 0.0005 0.0001 0.0001 0.0011 0.0015 0.0002 0.0007 0.0006 0.0007 0.001
Bud Periphery 0.0106 0.0066 0.0098 0.0162 0.012 0.0047 0.0028 0.0022 0.0067 0.0002 0.0272 0.0035 0.0039 0.0121 0.0073 0.007 0.0009 0.0035
Bud Site 0.0095 0.0141 0.0065 0.0029 0.0044 0.0008 0.0073 0.0123 0.01 0.0002 0.001 0.0013 0.0071 0.0172 0.0105 0.0062 0.0006 0.0004
Cell Periphery 0.0012 0.0035 0.0005 0.0003 0.0004 0.0003 0.0005 0.0002 0.0006 0.0001 0.0017 0.0003 0.0006 0.001 0.0013 0.0006 0.0001 0.0002
Cytoplasm 0.0956 0.2312 0.2267 0.2624 0.2507 0.2664 0.091 0.1224 0.1028 0.0527 0.1041 0.1364 0.3385 0.4403 0.3216 0.3684 0.1302 0.4572
Cytoplasmic Foci 0.0494 0.0076 0.0201 0.0191 0.0294 0.0151 0.0418 0.0044 0.0114 0.0053 0.0189 0.0104 0.0591 0.0075 0.0229 0.0247 0.0089 0.0226
Eisosomes 0.001 0.0007 0.0004 0.0008 0.0002 0.0002 0.0005 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0004 0.0001 0.0004 0
Endoplasmic Reticulum 0.0053 0.0014 0.0021 0.0047 0.0014 0.0031 0.0026 0.0003 0.001 0.0022 0.0032 0.001 0.0022 0.0012 0.0031 0.0019 0.0011 0.001
Endosome 0.0317 0.0004 0.0119 0.0182 0.0234 0.0046 0.0156 0.0018 0.0052 0.0239 0.0464 0.003 0.0233 0.0004 0.0067 0.0149 0.0221 0.0006
Golgi 0.0135 0.0001 0.0066 0.0008 0.0231 0.0021 0.0102 0.0113 0.0029 0.0054 0.0029 0.0031 0.0064 0.0001 0.006 0.0146 0.0574 0.0001
Lipid Particles 0.0169 0.0007 0.0112 0.0027 0.0097 0.005 0.0288 0.0005 0.0073 0.0055 0.0018 0.0061 0.0664 0.0003 0.0018 0.0216 0.0298 0.0003
Mitochondria 0.0131 0.0002 0.0021 0.004 0.0104 0.0159 0.0149 0.0016 0.0077 0.0089 0.0025 0.0026 0.0032 0.0021 0.0037 0.0079 0.0192 0.0005
None 0.6777 0.6965 0.6541 0.6179 0.5582 0.632 0.7018 0.8134 0.7922 0.8416 0.7495 0.7838 0.423 0.4718 0.5359 0.4409 0.6668 0.4906
Nuclear Periphery 0.0078 0.0014 0.0021 0.005 0.0026 0.0037 0.0022 0.0003 0.0018 0.0031 0.0039 0.0011 0.0028 0.0007 0.0019 0.0023 0.0013 0.0013
Nucleolus 0.0027 0.0004 0.0007 0.0021 0.0054 0.0008 0.0031 0.001 0.0007 0.0002 0.0004 0.0004 0.002 0.001 0.0008 0.0038 0.0002 0.0004
Nucleus 0.0048 0.0031 0.0048 0.0052 0.0152 0.0086 0.0071 0.008 0.0065 0.0005 0.0019 0.0043 0.0064 0.0042 0.0028 0.0105 0.0005 0.0062
Peroxisomes 0.011 0.0001 0.0098 0.0035 0.0108 0.0065 0.0354 0.0021 0.009 0.0439 0.0054 0.0154 0.0112 0.0004 0.002 0.0073 0.0045 0.0001
Punctate Nuclear 0.0062 0.0081 0.0076 0.0092 0.0102 0.0061 0.0139 0.0066 0.0028 0.0006 0.0039 0.0118 0.0204 0.0013 0.0069 0.0492 0.001 0.0034
Vacuole 0.0142 0.0031 0.0024 0.0059 0.0047 0.0066 0.0021 0.0004 0.0019 0.0014 0.0053 0.0011 0.0071 0.0037 0.005 0.0028 0.0008 0.0015
Vacuole Periphery 0.0031 0.0002 0.0005 0.0019 0.0008 0.0013 0.0006 0.0001 0.0008 0.0014 0.0021 0.0002 0.0018 0.0008 0.0008 0.0008 0.0011 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.0963 5.1697 3.8361 8.5207 4.4988 6.3578 8.6247 10.0546 10.5889 8.8547
Translational Efficiency 1.2708 1.1356 1.6343 1.0729 1.9423 1.3549 1.3268 1.0911 1.068 0.8787

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1591 1634 494 1432 1999 2216 227 2 3590 3850 721 1434

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 661.18 1360.82 5636.81 5630.45 735.92 976.50 4627.27 285.25 702.80 1139.61 5318.97 5623.00
Standard Deviation 196.98 914.72 4806.20 1776.86 274.56 490.41 2473.12 2.18 246.07 727.75 4239.39 1786.79
Intensity Change Log 2 — 1.041361 3.091764 3.090135 — 0.408071 2.652540 -1.367322 — 0.742420 2.877097 2.082114

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000266 0.000441 0.000690 0.000278 0.000141 0.000238 0.000351 0.000010 0.000196 0.000324 0.000583 0.000277
Bud Neck 0.018355 0.003884 0.003625 0.003492 0.011820 0.008037 0.006909 0.000001 0.014717 0.006274 0.004659 0.003487
Bud Site 0.021000 0.024975 0.007172 0.009416 0.011109 0.019495 0.004613 0.000007 0.015493 0.021821 0.006367 0.009403
Cell Periphery 0.000643 0.001197 0.010973 0.002012 0.000354 0.000175 0.006851 0.000060 0.000482 0.000609 0.009675 0.002009
Cytoplasm 0.606183* 0.787935* 0.716888* 0.726338* 0.567785* 0.785822* 0.655553* 0.000009 0.584802* 0.786719* 0.697577* 0.725325*
Cytoplasmic Foci 0.188140* 0.056061 0.036091 0.007828 0.183830* 0.070858 0.030289 0.000002 0.185740* 0.064578 0.034264 0.007817
Eisosomes 0.000233 0.000016 0.000006 0.000004 0.000113 0.000020 0.000006 0.000031 0.000166 0.000018 0.000006 0.000004
Endoplasmic Reticulum 0.001812 0.001313 0.000067 0.000182 0.001423 0.001175 0.000106 0.000000 0.001595 0.001233 0.000079 0.000182
Endosome 0.007363 0.006474 0.000316 0.001653 0.008003 0.007292 0.001238 0.000000 0.007720 0.006945 0.000606 0.001651
Golgi 0.003860 0.002995 0.005505 0.004168 0.002119 0.002538 0.006560 0.000001 0.002891 0.002732 0.005837 0.004163
Lipid Particles 0.006934 0.002127 0.007588 0.005689 0.007899 0.000960 0.016740 0.000002 0.007471 0.001456 0.010470 0.005681
Mitochondria 0.008261 0.001361 0.000244 0.000423 0.004948 0.002038 0.000139 0.000006 0.006417 0.001751 0.000211 0.000422
Mitotic Spindle 0.000580 0.011439 0.001282 0.006266 0.002983 0.009243 0.002730 0.000024 0.001918 0.010175 0.001738 0.006257
None 0.019723 0.014979 0.006219 0.005655 0.008691 0.009539 0.002370 0.999822* 0.013580 0.011847 0.005007 0.007042
Nuclear Periphery 0.000214 0.000311 0.000821 0.000221 0.000447 0.000170 0.000948 0.000000 0.000344 0.000230 0.000861 0.000220
Nuclear Periphery Foci 0.000571 0.003188 0.004212 0.002586 0.000613 0.000872 0.009895 0.000000 0.000594 0.001855 0.006001 0.002582
Nucleolus 0.001446 0.000558 0.000166 0.000578 0.001094 0.000687 0.000154 0.000001 0.001250 0.000632 0.000162 0.000577
Nucleus 0.079044 0.037365 0.140079* 0.062845 0.143149* 0.035399 0.130249* 0.000002 0.114739 0.036233 0.136984* 0.062757
Peroxisomes 0.002316 0.000821 0.000036 0.000384 0.004355 0.001739 0.000035 0.000023 0.003452 0.001349 0.000036 0.000383
Vacuole 0.030099 0.040493 0.053769 0.155465 0.038021 0.041491 0.122053 0.000000 0.034510 0.041067 0.075267 0.155248
Vacuole Periphery 0.002958 0.002067 0.004251 0.004518 0.001101 0.002213 0.002210 0.000000 0.001924 0.002151 0.003609 0.004512

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.85 -1.11 0.35 2.40 1.17 NaN NaN NaN NaN NaN -2.70 -1.63 -1.19 1.78 1.47
Bud Neck 10.10 8.30 8.57 -2.99 0.45 NaN NaN NaN NaN NaN 9.49 7.38 9.85 0.81 3.10
Bud Site -1.60 5.74 5.29 7.63 -0.97 NaN NaN NaN NaN NaN -4.48 5.82 3.81 7.95 -1.55
Cell Periphery -2.06 -8.07 -6.91 -1.32 7.24 NaN NaN NaN NaN NaN -1.21 -9.82 -8.55 -5.66 8.46
Cytoplasm -20.54 -3.67 -5.27 13.64 -0.18 NaN NaN NaN NaN NaN -34.25 -3.85 -8.50 15.75 -2.60
Cytoplasmic Foci 25.03 22.28 39.13 17.39 8.43 NaN NaN NaN NaN NaN 32.68 30.55 54.39 27.15 11.09
Eisosomes 15.74 16.34 16.70 8.37 4.11 NaN NaN NaN NaN NaN 22.19 23.69 24.57 14.01 6.04
Endoplasmic Reticulum 1.62 8.28 8.04 4.28 -1.40 NaN NaN NaN NaN NaN 1.39 8.40 8.18 4.93 -0.94
Endosome 1.56 16.67 13.26 8.19 -2.86 NaN NaN NaN NaN NaN 1.70 22.17 17.31 11.47 -1.53
Golgi 1.60 -2.87 2.05 0.08 4.17 NaN NaN NaN NaN NaN 0.56 -6.53 0.10 -0.34 5.06
Lipid Particles 7.78 -3.54 0.12 -7.33 3.57 NaN NaN NaN NaN NaN 13.30 -6.28 0.96 -9.92 6.63
Mitochondria 5.73 7.00 6.94 2.51 -0.57 NaN NaN NaN NaN NaN 6.30 8.91 8.73 4.93 -0.99
Mitotic Spindle -6.50 -3.27 -3.08 3.92 -2.22 NaN NaN NaN NaN NaN -7.10 -1.07 -1.74 4.13 -0.93
None 2.32 7.41 8.34 6.80 2.03 NaN NaN NaN NaN NaN 1.74 8.66 5.69 4.63 -0.45
Nuclear Periphery -1.77 -8.57 -4.49 -0.47 7.29 NaN NaN NaN NaN NaN 3.18 -10.34 -0.26 -3.32 10.20
Nuclear Periphery Foci -5.86 -10.38 -9.86 0.78 5.21 NaN NaN NaN NaN NaN -6.45 -15.21 -10.18 -3.23 9.10
Nucleolus 3.76 6.29 4.34 0.67 -2.94 NaN NaN NaN NaN NaN 4.46 10.19 5.45 1.31 -2.92
Nucleus 12.52 -11.40 -1.70 -12.34 9.81 NaN NaN NaN NaN NaN 30.16 -6.39 6.87 -13.75 10.60
Peroxisomes 7.01 12.31 10.22 4.34 -1.51 NaN NaN NaN NaN NaN 5.37 9.14 8.48 7.36 -1.47
Vacuole -4.76 -8.06 -26.35 -23.31 -13.44 NaN NaN NaN NaN NaN -4.92 -12.37 -25.95 -24.22 -10.12
Vacuole Periphery 1.87 -0.94 -0.48 -2.92 0.68 NaN NaN NaN NaN NaN -0.49 -2.17 -3.57 -2.82 -0.28
Standard name
Human Ortholog
Description Phenylpyruvate decarboxylase; catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway; involved in protein N-terminal Met and Ala catabolism
Localization
Cell Percentages cytoplasm (43%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Aro10

Aro10


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aro10-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available