Standard name
Human Ortholog
Description Ribosomal protein P2 beta; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.99 0.97 0.99 0.96 0.97 – 0.88 0.91 0.81 0.87 0.82 – – – 0.97 0.99 0.98 0.96 0.93 0.83 0.85 0.91 0.96
Endoplasmic Reticulum 0 0 0 0 0 0 – 0.09 0.08 0.15 0.08 0.13 – – – 0.09 0 0.09 0 0 0.07 0.08 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 1 0 1 1 – – – 0 0 1 0 0 0 0 0 0
Bud 0 0 2 0 0 0 – 0 0 1 1 0 – – – 0 0 1 0 1 1 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 2 1 – 3 3 8 3 6 – – – 3 1 3 0 0 0 0 0 0
Cytoplasm 222 152 154 126 176 197 – 209 161 204 116 130 – – – 177 141 174 208 144 136 11 19 31
Endoplasmic Reticulum 0 1 0 1 3 5 – 22 15 38 11 21 – – – 16 6 16 1 2 11 1 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 1 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 2 2 0 0 2 – – – 1 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 – 0 0 0 1 3 – – – 3 3 10 0 0 3 0 0 0
Nucleus 1 0 1 0 1 0 – 0 0 0 0 0 – – – 0 0 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 – 3 1 1 2 0 – – – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 – – – 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 0 1 0 – 2 0 0 1 0 – – – 2 2 0 0 0 2 0 0 0
Unique Cell Count 223 153 158 127 183 203 238 177 251 133 159 183 143 178 218 156 164 13 21 33
Labelled Cell Count 223 153 159 127 184 203 241 183 252 136 163 202 154 205 218 156 164 13 21 33


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 258.1 297.3 157.9 218.7 187.1 140.6 – 115.5 111.9 104.2 91.8 91.0 – – – 324.0 305.9 317.4 – – –
Std Deviation (1e-4) 48.9 51.3 59.8 65.3 54.1 36.7 – 35.7 27.8 24.6 27.8 19.9 – – – 115.5 108.8 82.7 – – –
Intensity Change (Log2) – – – 0.47 0.24 -0.17 – -0.45 -0.5 -0.6 -0.78 -0.8 – – – 1.04 0.95 1.01 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Bud 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Cytoplasm 1.1 -0.7 -0.2 – -3.4 -2.5 -4.8 -3.4 -4.6 – – – -0.4 0.7 0.2
Endoplasmic Reticulum 0 0 0 – 3.9 3.7 5.1 3.7 4.7 – – – 3.8 0 3.9
Endosome 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Mitochondria 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Nucleus 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 – – – 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 – – – 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 – – – 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 220.4198 306.9932 240.9078 197.4836 248.4959 310.9334 220.6058 307.1096 245.7365 208.2774 274.7616 209.936 254.7654 350.7749 304.3912 251.6166 302.2852 303.9749
Actin 0.0078 0.0044 0.0074 0.0598 0.002 0.0096 0.0109 0.0013 0.0073 0.0407 0.0036 0.0004 0.0084 0.0016 0.009 0.0023 0.0014 0.0095
Bud 0.0074 0.0086 0.01 0.0096 0.006 0.0079 0.0059 0.0035 0.0088 0.0108 0.003 0.0016 0.0064 0.0052 0.0043 0.0082 0.0051 0.0168
Bud Neck 0.0016 0.0023 0.003 0.0009 0.0048 0.0034 0.0027 0.0023 0.0023 0.0047 0.004 0.0005 0.0013 0.0017 0.003 0.0032 0.009 0.0063
Bud Periphery 0.0137 0.0074 0.0217 0.0081 0.0053 0.0072 0.0111 0.0042 0.0113 0.0166 0.0052 0.0013 0.0098 0.0035 0.003 0.0042 0.0027 0.0136
Bud Site 0.0044 0.0045 0.0058 0.0036 0.001 0.003 0.0039 0.0038 0.007 0.0028 0.0005 0.0007 0.0029 0.0042 0.0037 0.0023 0.0011 0.0024
Cell Periphery 0.1065 0.0797 0.0933 0.0375 0.032 0.0209 0.1371 0.0527 0.1228 0.0446 0.016 0.0116 0.0864 0.0644 0.1305 0.059 0.0121 0.065
Cytoplasm 0.285 0.5769 0.3753 0.1784 0.3391 0.3444 0.3401 0.7603 0.3711 0.2894 0.6266 0.2264 0.4525 0.746 0.5026 0.4835 0.5038 0.3193
Cytoplasmic Foci 0.009 0.0018 0.0024 0.0019 0.0259 0.005 0.007 0.0008 0.005 0.0014 0.0049 0.0048 0.0099 0.001 0.0035 0.0033 0.0051 0.0033
Eisosomes 0.0035 0.0031 0.0014 0.0005 0.002 0.0006 0.0037 0.0002 0.0075 0.0006 0.0004 0.0006 0.0027 0.0001 0.0019 0.0088 0.0001 0.0022
Endoplasmic Reticulum 0.0292 0.01 0.0244 0.0267 0.0047 0.1086 0.037 0.0126 0.0046 0.0159 0.0207 0.0005 0.0214 0.003 0.01 0.0017 0.0084 0.0199
Endosome 0.0106 0.0036 0.0183 0.0167 0.0039 0.0167 0.0046 0.0019 0.0041 0.0087 0.012 0.002 0.009 0.0016 0.005 0.0021 0.0127 0.0068
Golgi 0.0022 0.0011 0.0057 0.0224 0.0007 0.0049 0.0013 0.0005 0.001 0.0027 0.0062 0.0002 0.0021 0.0004 0.0017 0.0006 0.0009 0.0034
Lipid Particles 0.0065 0.0039 0.0031 0.0031 0.0113 0.0016 0.006 0.001 0.0063 0.0018 0.007 0.0011 0.0071 0.0013 0.0049 0.0058 0.0019 0.005
Mitochondria 0.0558 0.0267 0.0515 0.0418 0.0173 0.2064 0.0492 0.0033 0.0251 0.0166 0.0584 0.0366 0.0412 0.0113 0.0198 0.0151 0.0056 0.1044
None 0.0099 0.0037 0.0033 0.001 0.0545 0.0048 0.0122 0.0006 0.0072 0.001 0.0021 0.0088 0.0139 0.0009 0.006 0.0058 0.0019 0.0046
Nuclear Periphery 0.0761 0.0173 0.0259 0.0212 0.044 0.0867 0.0652 0.0141 0.0325 0.0389 0.0242 0.0149 0.0523 0.0089 0.0329 0.0325 0.0294 0.0969
Nucleolus 0.0279 0.017 0.021 0.0121 0.0107 0.0171 0.0191 0.0026 0.0268 0.0064 0.0102 0.2056 0.0109 0.0034 0.013 0.0334 0.0059 0.0269
Nucleus 0.058 0.0175 0.033 0.0169 0.0165 0.0225 0.0451 0.0151 0.0448 0.0258 0.0212 0.0679 0.0349 0.0098 0.0205 0.0352 0.0268 0.0532
Peroxisomes 0.0013 0.0008 0.001 0.001 0.0018 0.0051 0.0013 0.0001 0.0008 0.0007 0.0006 0.0007 0.0017 0.0003 0.0009 0.0011 0.0008 0.0015
Punctate Nuclear 0.0079 0.0002 0.0002 0.0002 0.0015 0.0006 0.0015 0.0001 0.0007 0.0003 0.0003 0.0009 0.0016 0 0.0007 0.0003 0.0002 0.0007
Vacuole 0.191 0.1652 0.1989 0.3576 0.266 0.049 0.183 0.1054 0.2375 0.2964 0.0844 0.1587 0.1656 0.1123 0.1804 0.1835 0.209 0.1298
Vacuole Periphery 0.0847 0.0443 0.0933 0.1789 0.1492 0.074 0.0521 0.0137 0.0656 0.1733 0.0883 0.2541 0.0583 0.0189 0.0427 0.1079 0.1562 0.1084

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3624.9984 4377.9699 5422.4636 2796.3617 3851.4827 5076.8246 3100.5393 2716.7626 3788.0054 2384.5507
Translational Efficiency 1.0935 1.032 0.8968 2.7349 1.4492 0.6813 1.1381 1.7792 1.0269 1.3238

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
97 1470 2444 1567 890 1236 1604 1156 987 2706 4048 2723

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 10921.96 14771.38 20598.43 17852.25 8516.82 14345.31 18909.01 16172.84 8753.19 14576.77 19929.01 17139.29
Standard Deviation 2810.53 3689.08 4014.21 4366.77 2698.34 3332.12 4095.58 3626.36 2802.57 3536.89 4130.15 4152.74
Intensity Change Log 2 — 0.435573 0.915303 0.708874 — 0.752192 1.150687 0.925186 — 0.582909 1.023187 0.807661

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000146 0.000070 0.000110 0.000543 0.000096 0.000082 0.000198 0.000272 0.000101 0.000076 0.000145 0.000428
Bud Neck 0.001444 0.000353 0.000512 0.001130 0.000611 0.000639 0.000892 0.000849 0.000693 0.000484 0.000663 0.001011
Bud Site 0.010207 0.002151 0.002830 0.005665 0.002680 0.002401 0.002694 0.002480 0.003420 0.002265 0.002776 0.004313
Cell Periphery 0.101988* 0.057450 0.051855 0.099478 0.051114 0.042785 0.104322* 0.169324* 0.056114 0.050752 0.072645 0.129130*
Cytoplasm 0.164220* 0.272438* 0.320709* 0.172313* 0.320712* 0.261523* 0.185921* 0.136969* 0.305333* 0.267452* 0.267300* 0.157309*
Cytoplasmic Foci 0.013604 0.011849 0.010622 0.003410 0.005381 0.012022 0.003605 0.002835 0.006189 0.011928 0.007842 0.003166
Eisosomes 0.000008 0.000004 0.000004 0.000011 0.000004 0.000004 0.000007 0.000016 0.000004 0.000004 0.000005 0.000013
Endoplasmic Reticulum 0.001814 0.006666 0.005263 0.002815 0.001454 0.002762 0.003122 0.002397 0.001490 0.004883 0.004415 0.002638
Endosome 0.001654 0.001242 0.001057 0.001363 0.000826 0.001147 0.000674 0.000523 0.000907 0.001199 0.000905 0.001006
Golgi 0.102578 0.126702* 0.076141 0.032361 0.052179 0.135489* 0.033105 0.028463 0.057132 0.130716* 0.059088 0.030706
Lipid Particles 0.070022 0.042395 0.027235 0.043190 0.036059 0.037496 0.040295 0.055315 0.039396 0.040157 0.032410 0.048338
Mitochondria 0.000112 0.000403 0.000588 0.001358 0.000252 0.000267 0.000818 0.000385 0.000239 0.000341 0.000679 0.000945
Mitotic Spindle 0.001080 0.000924 0.001026 0.001163 0.000356 0.001073 0.000693 0.000444 0.000427 0.000992 0.000894 0.000858
None 0.000653 0.002159 0.001260 0.001225 0.002730 0.001617 0.001598 0.000901 0.002526 0.001912 0.001394 0.001087
Nuclear Periphery 0.001427 0.001745 0.001720 0.001287 0.000708 0.002152 0.002163 0.001951 0.000779 0.001931 0.001896 0.001569
Nuclear Periphery Foci 0.010894 0.014720 0.011177 0.007758 0.011747 0.017255 0.008952 0.008358 0.011663 0.015878 0.010295 0.008013
Nucleolus 0.000124 0.000410 0.000130 0.000216 0.000435 0.000157 0.000177 0.000167 0.000405 0.000295 0.000149 0.000195
Nucleus 0.003089 0.003315 0.002842 0.004726 0.010304 0.003706 0.003315 0.005091 0.009595 0.003493 0.003029 0.004881
Peroxisomes 0.000074 0.000192 0.000101 0.000133 0.000101 0.000025 0.000148 0.000143 0.000099 0.000116 0.000119 0.000137
Vacuole 0.330961* 0.212271* 0.245497* 0.483934* 0.339545* 0.249433* 0.464818* 0.482983* 0.338701* 0.229245* 0.332401* 0.483530*
Vacuole Periphery 0.183902* 0.242543* 0.239323* 0.135921* 0.162705* 0.227964* 0.142483* 0.100134* 0.164788* 0.235884* 0.200950* 0.120728*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.95 0.89 -2.60 -3.10 -2.77 1.40 -2.64 -3.29 -3.82 -0.82 2.44 -2.27 -3.51 -3.92 -2.93
Bud Neck 2.63 2.22 0.15 -4.95 -4.63 -0.15 -2.66 -1.86 -1.90 1.39 1.56 -0.53 -3.07 -5.26 -3.31
Bud Site 2.85 2.57 1.38 -6.00 -4.90 0.92 -0.45 0.51 -0.54 1.14 2.58 1.13 -1.60 -5.68 -3.79
Cell Periphery 3.76 4.30 0.66 -9.76 -12.03 2.52 -11.99 -21.77 -24.63 -11.64 1.71 -4.82 -17.92 -23.82 -16.74
Cytoplasm -4.00 -5.82 -0.54 8.93 15.02 4.29 10.47 14.14 11.04 5.06 3.23 3.39 12.71 13.79 15.34
Cytoplasmic Foci 0.61 0.95 3.19 9.57 10.39 -6.44 2.13 2.82 7.67 0.82 -7.16 -2.57 4.67 12.16 8.20
Eisosomes 2.80 2.74 -1.67 -4.95 -4.90 0.32 -4.32 -4.21 -4.30 -2.87 0.92 -1.93 -5.82 -6.39 -5.26
Endoplasmic Reticulum -5.54 -5.56 -1.41 5.04 5.27 -2.84 -3.80 -2.17 0.82 1.69 -6.66 -7.71 -2.83 4.87 5.53
Endosome 1.61 2.41 0.93 -0.28 -1.00 -3.37 1.80 4.46 8.09 2.38 -3.74 0.04 -0.43 1.42 -0.47
Golgi -2.20 2.37 6.49 25.85 20.27* -19.31 7.49 9.10 27.41 2.51 -21.48 -0.76 10.67 37.44 18.73*
Lipid Particles 2.50 3.94 2.60 0.44 -7.15 -0.48 -1.25 -5.50 -5.84 -5.62 -0.35 2.70 -2.69 -3.56 -9.25
Mitochondria -4.10 -9.14 -3.85 -3.00 -2.48 0.31 -2.50 -0.74 -0.99 1.83 -0.74 -3.69 -3.35 -3.18 -1.32
Mitotic Spindle 0.80 0.10 -0.14 -0.52 -0.20 -8.41 -3.02 -1.40 6.31 2.05 -10.34 -7.94 -1.71 0.60 0.29
None -3.13 -4.64 -2.51 1.61 -0.11 1.64 1.55 2.89 1.42 1.49 1.02 2.06 2.56 2.10 1.12
Nuclear Periphery -1.20 -1.36 0.49 4.22 5.79 -4.91 -9.15 -7.58 0.74 1.24 -7.71 -13.63 -9.23 2.33 3.67
Nuclear Periphery Foci -2.73 -0.34 2.33 10.77 7.59 -5.60 3.18 4.19 10.69 1.28 -5.56 1.63 5.23 15.20 6.64
Nucleolus -0.99 -0.42 -4.57 0.64 -6.73 1.97 1.81 1.99 -0.18 0.50 0.55 2.08 1.69 0.60 -2.85
Nucleus 0.02 0.02 -2.05 -2.91 -3.37 3.93 3.70 2.50 -1.65 -1.30 4.30 4.34 2.29 -3.29 -3.47
Peroxisomes -1.15 -1.26 -1.88 0.63 -1.06 3.06 -1.14 -0.64 -2.01 0.04 -0.30 -0.72 -0.97 -0.26 -0.51
Vacuole 4.56 3.20 -5.80 -32.29 -29.78 8.22 -11.57 -12.48 -24.31 -1.67 11.79 0.50 -14.93 -40.04 -23.62
Vacuole Periphery -3.13 -2.92 2.72 18.49 20.71* -8.25 3.18 9.04 20.74 8.77 -10.70 -5.63 7.35 27.12 22.12*
Standard name
Human Ortholog
Description Ribosomal protein P2 beta; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rpp2b

Rpp2b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpp2b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available