Standard name
Human Ortholog
Description Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.06 0 0.07 0.12 0.07 – 0.08 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.99 0.96 0.91 0.84 0.86 – 0.9 0.82 0.78 0.74 0.99 0.97 0.99 0.98 0.98 0.98 0.93 0.97 0.88 0.78 0.78 0.83
Endoplasmic Reticulum 0 0 0 0 0.06 0.06 0 – 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0.09 0.1 0.08
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.05 0.12 0.18 0.14 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 – 0.05 0.08 0.05 0.11 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 3 – 3 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 1 5 – 2 5 4 2 0 0 0 0 0 0 0 1 3 3 9 8
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 6 13 6 12 24 16 – 17 11 8 8 0 5 0 3 5 0 0 0 2 0 1 0
Cytoplasm 69 276 205 182 157 165 208 – 203 176 114 105 171 226 240 217 157 140 79 278 193 124 252 282
Endoplasmic Reticulum 0 1 2 6 10 12 7 – 2 2 2 0 1 3 1 10 6 9 1 2 5 14 30 27
Endosome 0 0 0 0 0 0 1 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0
Golgi 0 0 0 1 1 3 1 – 1 0 0 1 0 0 1 2 0 1 0 0 0 0 4 2
Mitochondria 0 0 0 0 0 0 3 – 11 25 27 20 0 1 0 0 0 0 0 0 0 2 3 3
Nucleus 0 0 0 0 2 0 2 – 2 2 3 3 0 0 0 0 0 0 1 0 2 0 0 1
Nuclear Periphery 0 1 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 2 – 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0
SpindlePole 0 0 0 0 0 1 2 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 1 0 4 16 – 11 18 7 16 0 1 0 1 0 2 1 1 4 3 7 6
Unique Cell Count 69 277 208 190 173 196 243 226 215 147 142 173 232 242 222 161 143 86 287 220 159 322 342
Labelled Cell Count 69 284 222 196 182 212 266 254 240 166 158 173 236 242 233 168 152 86 287 220 159 322 342


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 176.9 139.0 100.3 112.9 74.3 35.6 15.0 – 8.5 8.2 8.3 7.9 144.0 132.4 111.2 200.9 215.6 177.0 115.5 110.7 107.3
Std Deviation (1e-4) 46.2 36.2 32.7 36.5 25.6 18.3 7.1 – 3.9 3.2 3.2 4.7 48.8 37.7 35.7 68.2 68.7 71.4 48.5 42.1 44.0
Intensity Change (Log2) – – – 0.17 -0.43 -1.5 -2.74 – -3.56 -3.61 -3.6 -3.67 0.52 0.4 0.15 1.0 1.1 0.82 0.2 0.14 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.4 0.3 2.1 0.1 – 0.5 -0.5 -0.3 -0.2 -3.3 -2.2 -3.9 -2.7 -1.4 -3.0
Cytoplasm -1.7 -3.5 -5.2 -4.9 – -3.8 -5.7 -6.5 -7.1 0.2 -0.8 0.6 -0.6 -0.7 -0.5
Endoplasmic Reticulum 0 2.7 2.8 0 – 0 0 0 0 0 0 0 2.2 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 3.2 5.1 6.4 5.6 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.0 – 2.4 3.6 0 4.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 149.163 229.5001 201.1202 191.2439 164.1852 162.0249 174.9533 286.1682 260.2753 259.748 221.6409 240.4365 173.8485 258.4984 228.3718 205.6086 176.7723 244.644
Actin 0.0131 0.0006 0.0047 0.0549 0.004 0.0137 0.0139 0.0006 0.0014 0.0005 0.0069 0.0017 0.0136 0.0005 0.0118 0.0004 0.0004 0.0015
Bud 0.0035 0.0015 0.0023 0.0028 0.0043 0.0201 0.0079 0.0021 0.0034 0.0086 0.007 0.0088 0.0026 0.0025 0.0023 0.0027 0.0043 0.0035
Bud Neck 0.0014 0.0012 0.0019 0.0025 0.0019 0.0063 0.0011 0.0009 0.001 0.0013 0.0022 0.003 0.0011 0.0007 0.0014 0.0005 0.0008 0.0024
Bud Periphery 0.0037 0.0015 0.0017 0.0022 0.0072 0.0102 0.0102 0.0012 0.0044 0.0094 0.0102 0.0134 0.0027 0.0017 0.0031 0.0027 0.0031 0.0029
Bud Site 0.0053 0.0014 0.0066 0.0033 0.0012 0.0022 0.0036 0.0016 0.0015 0.0026 0.001 0.0005 0.0013 0.0013 0.0012 0.0008 0.0013 0.0004
Cell Periphery 0.0587 0.0558 0.0662 0.0554 0.0337 0.0127 0.0665 0.0365 0.0536 0.0371 0.0214 0.0336 0.0751 0.047 0.0578 0.0796 0.0354 0.0218
Cytoplasm 0.3798 0.7639 0.5588 0.4864 0.3806 0.4497 0.4477 0.8342 0.7273 0.4611 0.6178 0.5573 0.4902 0.8119 0.6558 0.6183 0.4742 0.7597
Cytoplasmic Foci 0.0517 0.0006 0.0223 0.003 0.006 0.0122 0.0169 0.0007 0.0058 0.0018 0.0025 0.0056 0.0201 0.001 0.0041 0.0014 0.0052 0.0034
Eisosomes 0.0027 0 0.0014 0.001 0.0001 0.0006 0.0039 0.0001 0.0004 0.0001 0.0006 0.0003 0.0016 0.0001 0.0004 0.0004 0.0001 0.0001
Endoplasmic Reticulum 0.0142 0.0051 0.0138 0.0016 0.0118 0.0251 0.0229 0.0024 0.0064 0.0043 0.0025 0.007 0.024 0.009 0.0138 0.0078 0.0029 0.0272
Endosome 0.0096 0.002 0.0046 0.0046 0.0217 0.0354 0.0122 0.001 0.0029 0.0044 0.0071 0.0152 0.0088 0.0011 0.0057 0.0018 0.0058 0.0083
Golgi 0.0026 0.0003 0.0012 0.0063 0.006 0.0067 0.0023 0.0001 0.0005 0.0002 0.0027 0.001 0.0039 0.0003 0.0024 0.0002 0.0005 0.0013
Lipid Particles 0.008 0.0012 0.0068 0.0044 0.0377 0.0045 0.0077 0.001 0.0032 0.0014 0.0026 0.0031 0.0137 0.0022 0.0038 0.0061 0.0025 0.0031
Mitochondria 0.0277 0.0018 0.0105 0.0114 0.0274 0.0589 0.0268 0.0009 0.0062 0.0048 0.0471 0.0075 0.0145 0.0023 0.0056 0.0063 0.0264 0.0043
None 0.0307 0.0004 0.0063 0.0019 0.0014 0.0111 0.0142 0.0008 0.0051 0.0018 0.0259 0.004 0.0128 0.0009 0.0067 0.0023 0.0034 0.0014
Nuclear Periphery 0.0483 0.0111 0.0199 0.0148 0.0099 0.0628 0.0677 0.0051 0.0094 0.016 0.0123 0.0115 0.0275 0.0062 0.0125 0.0073 0.0038 0.0134
Nucleolus 0.0197 0.0013 0.0101 0.0212 0.005 0.0533 0.0236 0.0033 0.0036 0.0053 0.02 0.0411 0.0401 0.0023 0.0095 0.0196 0.0763 0.0037
Nucleus 0.0467 0.0081 0.0116 0.0272 0.017 0.0696 0.0389 0.0116 0.0117 0.0199 0.0221 0.014 0.0206 0.0051 0.0083 0.012 0.0165 0.0135
Peroxisomes 0.0032 0.0001 0.0023 0.0009 0.0173 0.0014 0.0016 0.0001 0.0002 0.0002 0.0006 0.0004 0.0073 0.0001 0.0036 0.0002 0.0009 0.0002
Punctate Nuclear 0.0189 0 0.0025 0.0004 0.0019 0.0039 0.0064 0.0001 0.0002 0.0001 0.0003 0.0005 0.0014 0.0001 0.0011 0.0001 0.0001 0.0003
Vacuole 0.196 0.121 0.2087 0.223 0.3083 0.0911 0.1692 0.087 0.1372 0.3347 0.14 0.1989 0.1877 0.0947 0.1706 0.1837 0.2407 0.0978
Vacuole Periphery 0.0544 0.0212 0.0358 0.0707 0.0955 0.0485 0.0348 0.0088 0.0145 0.0847 0.047 0.0718 0.0291 0.0091 0.0185 0.0457 0.0954 0.0299

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4233.2074 4459.9492 4689.122 3752.9901 3976.1852 4012.6163 3927.0862 3409.9483 3784.5883 3217.135
Translational Efficiency 0.474 0.4979 0.5066 0.5992 0.5169 0.4444 0.5078 0.555 0.4662 0.5093

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1071 1283 808 1372 1469 2790 2035 462 2540 4073 2843 1834

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 614.50 670.54 807.28 783.91 658.49 696.19 776.78 798.33 639.94 688.11 785.45 787.54
Standard Deviation 74.81 115.77 91.45 113.27 72.60 88.76 92.23 124.88 76.68 98.79 93.03 116.47
Intensity Change Log 2 — 0.125910 0.393656 0.351275 — 0.080320 0.238345 0.277824 — 0.102507 0.315406 0.313747

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000121 0.000668 0.001610 0.001988 0.000105 0.000511 0.000290 0.000483 0.000112 0.000560 0.000665 0.001608
Bud Neck 0.015469 0.013122 0.008534 0.019561 0.011995 0.026828 0.017230 0.010821 0.013460 0.022511 0.014758 0.017359
Bud Site 0.004399 0.008786 0.017643 0.068301 0.001767 0.012999 0.024531 0.020298 0.002877 0.011672 0.022574 0.056208
Cell Periphery 0.000128 0.000135 0.000201 0.000269 0.000147 0.000102 0.000193 0.000194 0.000139 0.000112 0.000196 0.000250
Cytoplasm 0.293429* 0.236173* 0.136228* 0.359404* 0.291184* 0.177124* 0.377922* 0.414052* 0.292131* 0.195725* 0.309231* 0.373170*
Cytoplasmic Foci 0.413044* 0.322396* 0.011693 0.025904 0.368337* 0.359900* 0.021227 0.035933 0.387188* 0.348086* 0.018517 0.028431
Eisosomes 0.000143 0.000123 0.000093 0.000050 0.000155 0.000102 0.000048 0.000060 0.000150 0.000109 0.000061 0.000052
Endoplasmic Reticulum 0.000539 0.000896 0.004304 0.002639 0.000703 0.000456 0.001160 0.003383 0.000634 0.000594 0.002053 0.002826
Endosome 0.023543 0.035811 0.005045 0.012380 0.018454 0.032614 0.004217 0.012802 0.020600 0.033621 0.004452 0.012487
Golgi 0.006129 0.011223 0.000895 0.010604 0.005309 0.016277 0.001146 0.015382 0.005655 0.014685 0.001074 0.011808
Lipid Particles 0.034176 0.036034 0.003648 0.004101 0.055283 0.038156 0.002069 0.003276 0.046383 0.037488 0.002518 0.003893
Mitochondria 0.011467 0.016242 0.001161 0.007176 0.009491 0.027371 0.001495 0.007868 0.010324 0.023865 0.001400 0.007351
Mitotic Spindle 0.025717 0.123261* 0.192932* 0.181207* 0.030822 0.176617* 0.104283* 0.092031 0.028670 0.159810* 0.129478* 0.158743*
None 0.009833 0.015087 0.016483 0.005248 0.015893 0.006401 0.021394 0.008653 0.013338 0.009137 0.019998 0.006106
Nuclear Periphery 0.000881 0.001395 0.002010 0.002791 0.001364 0.000874 0.001846 0.006377 0.001160 0.001038 0.001893 0.003694
Nuclear Periphery Foci 0.003358 0.006730 0.010434 0.003029 0.003899 0.002069 0.002071 0.015094 0.003671 0.003538 0.004448 0.006068
Nucleolus 0.012107 0.022637 0.052482 0.001983 0.020880 0.013776 0.013283 0.004064 0.017181 0.016568 0.024424 0.002507
Nucleus 0.099991 0.087901 0.512591* 0.196855* 0.126411 0.043252 0.375889* 0.204571* 0.115271 0.057316 0.414741* 0.198799*
Peroxisomes 0.027667 0.038881 0.003205 0.004340 0.022962 0.048207 0.001370 0.001652 0.024946 0.045270 0.001891 0.003663
Vacuole 0.017066 0.020420 0.018418 0.083806 0.014159 0.014827 0.028024 0.133580 0.015385 0.016589 0.025294 0.096344
Vacuole Periphery 0.000793 0.002079 0.000390 0.008365 0.000680 0.001536 0.000313 0.009424 0.000728 0.001707 0.000335 0.008632

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.35 -12.06 -9.14 -3.81 3.09 -3.62 -14.84 -11.27 -4.99 -3.71 -4.24 -17.48 -10.92 -6.30 -1.93
Bud Neck 0.36 -3.27 -5.09 -6.20 -2.29 -9.38 -6.92 -2.05 5.38 3.52 -8.17 -7.38 -6.58 0.58 0.17
Bud Site -2.81 -5.76 -10.63 -8.99 -4.49 -9.06 -10.54 -5.90 -2.85 0.13 -8.85 -11.77 -12.34 -8.88 -4.20
Cell Periphery -0.66 -4.60 -4.37 -4.27 -0.27 4.06 -3.48 -3.80 -6.44 -1.47 2.41 -5.43 -5.57 -7.67 -1.68
Cytoplasm 2.07 17.49 -5.67 -8.33 -26.76 5.96 -0.42 -3.64 -7.58 -3.49 6.32 6.18 -7.40 -13.91 -13.41
Cytoplasmic Foci 12.34 52.31 49.61 39.99 -7.25 3.39 49.57 47.45 57.71 1.84 9.85 69.92 69.81 75.20 1.07
Eisosomes 1.68 -4.94 3.86 2.27 7.26 6.50 3.66 1.12 -4.14 -1.67 6.59 0.12 4.91 -1.93 4.25
Endoplasmic Reticulum -2.72 -18.24 -18.18 -17.76 4.87 1.86 -18.79 -12.42 -12.72 -7.03 -0.47 -25.11 -22.47 -22.72 -4.94
Endosome -4.75 8.11 10.10 14.65 2.30 -7.85 7.96 7.41 13.85 1.35 -8.61 11.18 13.92 22.06 3.84
Golgi -3.75 7.02 3.51 6.32 -0.90 -9.56 8.77 1.38 5.77 -0.93 -10.07 11.16 3.70 10.54 -1.57
Lipid Particles 1.33 13.40 13.24 13.08 -0.16 3.62 16.37 15.95 18.66 -1.59 3.32 20.80 20.28 22.47 -1.24
Mitochondria -1.90 5.09 3.48 5.51 -4.64 -8.96 5.41 3.72 12.56 -0.58 -8.75 7.40 5.10 14.62 -3.82
Mitotic Spindle -11.41 -18.61 -10.12 1.29 10.90 -16.39* -19.33 -4.45 4.51 7.96 -20.23 -26.46 -11.38 3.85 12.06
None -2.12 0.26 2.15 4.58 2.51 6.79 -0.16 3.97 -1.23 4.66 4.61 -0.61 5.18 0.75 6.93
Nuclear Periphery -2.04 -13.51 -16.51 -14.41 -9.22 1.79 -14.80 -7.12 -7.59 -2.45 -0.06 -19.81 -17.49 -17.54 -8.62
Nuclear Periphery Foci -0.92 -8.83 0.92 1.99 9.64 6.59 -2.48 -0.81 -4.55 0.85 4.33 -7.79 0.72 -2.97 7.95
Nucleolus -2.63 -12.20 9.17 12.20 18.01 9.00 -4.52 13.59 7.78 18.43* 6.18 -10.89 16.22 13.51 25.30*
Nucleus 0.27 -19.40 -25.75 -26.30 2.50 11.27 -26.02 -13.71 -18.06 1.87 9.65 -33.17 -28.88 -35.44 2.42
Peroxisomes -3.20 16.57 17.72 17.96 2.32 -8.82 19.61 21.64 30.31* 5.29 -9.00 25.69 26.95 34.24 4.02
Vacuole -2.89 -5.09 -24.95 -24.13 -22.49 -2.49 -12.78 -17.78 -17.60 -15.21 -3.47 -13.55 -30.54 -30.03 -25.06
Vacuole Periphery -3.77 1.46 -2.52 -0.97 -2.75 -6.15 3.79 -1.65 -0.80 -1.86 -6.97 3.87 -3.06 -1.44 -3.42
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Eft2

Eft2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Eft2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available