ORF
Human Ortholog
Description Putative protein of unknown function; possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.93 0.99 1.0 0.88 0.92 0.84 0.79 – 0.7 0.79 0.72 0.69 0.99 0.98 1.0 0.92 0.77 0.92 0.92 0.93 0.88 0.93 0.91 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.1 0.05 – 0.18 0.09 0.19 0.23 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0.07 0.06 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0 0 0.08 0.1 0.09 0.19 – 0.15 0.1 0.09 0.14 0 0 0 0.12 0.27 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 3
Bud 0 1 0 0 0 1 0 – 0 0 1 1 0 0 0 0 0 0 1 1 0 2 4 9
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 0 0 0 0 0 2 – 0 1 2 7 0 1 1 1 1 3 0 0 0 0 0 0
Cytoplasm 166 181 16 23 88 78 148 – 74 93 134 148 230 393 393 129 23 76 184 165 53 288 247 271
Endoplasmic Reticulum 0 0 0 1 0 2 4 – 0 5 3 2 0 1 0 4 0 3 0 0 1 1 3 2
Endosome 1 0 0 0 0 0 1 – 0 0 0 0 0 3 0 1 0 0 1 1 0 1 2 3
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 2 0 2
Mitochondria 2 2 0 0 1 9 10 – 19 11 36 50 1 0 0 2 0 3 2 1 1 1 0 4
Nucleus 1 2 0 0 1 0 7 – 7 7 8 7 0 0 2 4 1 6 0 3 0 1 0 1
Nuclear Periphery 2 0 0 0 1 2 1 – 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 1 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 1 0 0 0 4 2
Vac/Vac Membrane 9 0 0 2 10 8 35 – 16 12 16 30 1 4 1 17 8 5 1 1 0 2 3 1
Unique Cell Count 179 183 16 26 96 93 187 106 118 186 215 232 399 393 140 30 83 200 177 61 311 273 312
Labelled Cell Count 186 186 16 26 101 100 208 118 129 202 246 232 402 397 158 33 96 200 177 61 311 273 312


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.8 7.4 6.1 10.0 10.8 6.6 9.5 – 7.8 7.3 7.5 7.3 8.6 8.7 9.7 10.5 10.3 11.0 7.0 7.7 8.1
Std Deviation (1e-4) 8.4 1.5 1.0 2.3 2.8 2.2 3.1 – 2.3 2.0 1.9 2.0 1.8 2.2 2.4 3.0 2.8 3.6 1.8 2.3 2.4
Intensity Change (Log2) – – – 0.73 0.83 0.12 0.65 – 0.36 0.26 0.3 0.26 0.5 0.52 0.67 0.79 0.76 0.85 0.2 0.34 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.4 -1.2 -1.7 -2.0 – -2.6 -2.0 -2.5 -2.7 -0.4 -0.5 0 -1.2 -2.1 -1.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.6451 1.5645 1.4094 0.374 1.317 1.0815 0.9278 1.8901 1.5008 1.6584 0.5741 1.2898 4.2769 5.9253 4.9171 5.056 4.7094 5.0704
Actin 0.0188 0.0226 0.0054 0.0038 0.0116 0.0059 0.0188 0.0016 0.0015 0.0175 0.0005 0.008 0.0016 0.0003 0.0006 0.0002 0.0009 0.0012
Bud 0.0008 0.0005 0.0003 0.0004 0.0003 0.0016 0.0036 0.009 0.0016 0.0026 0 0.0006 0.0001 0.0004 0.0003 0.0001 0.0006 0.0008
Bud Neck 0.0021 0.0001 0.0004 0.0005 0.0007 0.0072 0.0078 0.0005 0.0009 0.0095 0.0003 0.0016 0.0001 0.0001 0.0002 0.0002 0.0005 0.0007
Bud Periphery 0.0009 0.0003 0.0003 0.0004 0.0002 0.0012 0.0028 0.0065 0.0017 0.0033 0 0.0006 0.0001 0.0001 0.0002 0 0.0005 0.0012
Bud Site 0.0037 0.0006 0.0014 0.001 0.0007 0.0003 0.0064 0.0097 0.0083 0.0271 0 0.0003 0.0003 0.0005 0.0006 0.0002 0.0005 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0006 0.001 0.0001 0.001 0 0.0001 0.0001 0.0001 0.0001 0 0 0
Cytoplasm 0.5456 0.6825 0.6944 0.6035 0.7633 0.7648 0.5557 0.7552 0.7214 0.5883 0.8251 0.7347 0.7242 0.8445 0.8146 0.6738 0.8138 0.8055
Cytoplasmic Foci 0.0536 0.0068 0.0138 0.0241 0.0096 0.0105 0.0575 0.0208 0.022 0.0383 0.004 0.0215 0.0046 0.0022 0.0044 0.0035 0.0099 0.006
Eisosomes 0.0004 0 0.0001 0 0.0001 0 0.0003 0.0001 0 0.0002 0 0.0001 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0065 0.0024 0.0051 0.0061 0.0028 0.0023 0.005 0.0024 0.0019 0.0024 0.0015 0.003 0.002 0.0011 0.0024 0.0019 0.0022 0.0021
Endosome 0.0247 0.0009 0.0107 0.0278 0.0089 0.0061 0.0664 0.0101 0.0065 0.0385 0.001 0.0174 0.0011 0.0006 0.0012 0.001 0.0025 0.0018
Golgi 0.0075 0.0005 0.0024 0.0054 0.0026 0.0012 0.0158 0.0026 0.0012 0.0202 0.0001 0.0028 0.0005 0.0001 0.0001 0.0001 0.0002 0.0002
Lipid Particles 0.0166 0.0001 0.0049 0.002 0.0057 0.0003 0.03 0.0014 0.0006 0.0169 0 0.0031 0.0017 0.0001 0.0001 0.0001 0.0001 0.0001
Mitochondria 0.013 0.0002 0.0147 0.003 0.0005 0.0006 0.0207 0.0033 0.0008 0.0183 0.0001 0.0112 0.0001 0.0001 0.0001 0.0001 0.0002 0.0039
None 0.2469 0.2759 0.2197 0.2891 0.1733 0.1838 0.1705 0.1637 0.2221 0.165 0.1654 0.1859 0.2536 0.1474 0.1708 0.3136 0.1542 0.1694
Nuclear Periphery 0.0087 0.0008 0.0043 0.015 0.0042 0.0011 0.0035 0.0006 0.001 0.001 0.0003 0.0013 0.0005 0.0004 0.0007 0.0007 0.0017 0.0007
Nucleolus 0.0013 0 0.0013 0.0003 0.0003 0.0002 0.0009 0.0008 0.0002 0.0012 0 0.0001 0 0 0 0 0.0001 0
Nucleus 0.0066 0.0026 0.0044 0.0096 0.0047 0.009 0.0092 0.0019 0.0021 0.0026 0.001 0.0018 0.0025 0.0016 0.0023 0.0034 0.007 0.0043
Peroxisomes 0.0262 0.0014 0.0075 0.0015 0.0014 0.0002 0.0133 0.0015 0.0011 0.0284 0.0001 0.0022 0.0056 0 0.0001 0 0.0005 0.0004
Punctate Nuclear 0.0122 0.0007 0.0061 0.002 0.0078 0.0019 0.0025 0.0014 0.0013 0.0017 0.0002 0.0017 0.0008 0.0002 0.0004 0.0005 0.0037 0.0009
Vacuole 0.0028 0.0006 0.0018 0.0028 0.0008 0.0014 0.0069 0.0051 0.003 0.0144 0.0002 0.0017 0.0004 0.0003 0.0005 0.0004 0.0007 0.0006
Vacuole Periphery 0.0009 0.0001 0.0009 0.0015 0.0003 0.0003 0.0018 0.0008 0.0005 0.0016 0 0.0004 0 0.0001 0.0001 0.0001 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.3066 16.9335 21.9894 30.9164 22.5132 50.4855 24.6121 31.297 38.2029 25.7645
Translational Efficiency 1.5633 1.5207 1.6547 1.391 1.1384 1.4592 1.9532 1.7267 1.6979 1.4418

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1466 760 2573 1904 1412 2785 1330 95 2878 3545 3903 1999

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 894.56 810.41 1233.03 1122.89 785.28 865.24 1076.77 1080.46 840.95 853.49 1179.78 1120.87
Standard Deviation 128.95 136.00 244.50 236.48 107.33 116.39 213.57 218.64 130.79 122.94 245.84 235.83
Intensity Change Log 2 — -0.142526 0.462958 0.327966 — 0.139893 0.455431 0.460367 — -0.003603 0.459444 0.391375

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000332 0.000590 0.000113 0.002780 0.000068 0.000190 0.000309 0.006500 0.000203 0.000276 0.000180 0.002956
Bud Neck 0.011414 0.005607 0.001876 0.004346 0.003038 0.007967 0.001706 0.001666 0.007305 0.007461 0.001818 0.004218
Bud Site 0.006436 0.012385 0.004694 0.024889 0.002482 0.011725 0.005735 0.013850 0.004496 0.011867 0.005048 0.024364
Cell Periphery 0.000084 0.000125 0.000049 0.000367 0.000065 0.000059 0.000118 0.000298 0.000075 0.000073 0.000073 0.000364
Cytoplasm 0.679646* 0.662278* 0.672040* 0.673391* 0.727407* 0.735278* 0.644136* 0.529955* 0.703078* 0.719627* 0.662531* 0.666575*
Cytoplasmic Foci 0.070046 0.133660 0.003514 0.012185 0.090129 0.070146 0.008392 0.055337 0.079899 0.083763 0.005176 0.014236
Eisosomes 0.000046 0.000051 0.000003 0.000025 0.000030 0.000029 0.000019 0.000108 0.000038 0.000034 0.000009 0.000029
Endoplasmic Reticulum 0.000448 0.001093 0.000313 0.000982 0.000568 0.000290 0.001623 0.002969 0.000507 0.000462 0.000759 0.001076
Endosome 0.002425 0.011035 0.000248 0.003025 0.003756 0.004780 0.002055 0.008984 0.003078 0.006121 0.000863 0.003309
Golgi 0.001004 0.001058 0.000082 0.005049 0.000435 0.001623 0.000293 0.000156 0.000725 0.001502 0.000154 0.004816
Lipid Particles 0.001333 0.002343 0.000167 0.000485 0.001375 0.000990 0.000624 0.003963 0.001354 0.001280 0.000323 0.000650
Mitochondria 0.002124 0.000728 0.000327 0.001478 0.001777 0.002427 0.000370 0.000143 0.001954 0.002063 0.000342 0.001415
Mitotic Spindle 0.001752 0.001034 0.005879 0.026178 0.002292 0.007808 0.006196 0.000710 0.002017 0.006356 0.005987 0.024967
None 0.007056 0.005069 0.011067 0.010609 0.004290 0.004324 0.010956 0.002664 0.005699 0.004484 0.011029 0.010231
Nuclear Periphery 0.000167 0.000329 0.000387 0.000587 0.000224 0.000223 0.000676 0.000939 0.000195 0.000246 0.000486 0.000603
Nuclear Periphery Foci 0.000310 0.001843 0.000470 0.001249 0.000729 0.000266 0.003020 0.005783 0.000516 0.000604 0.001339 0.001465
Nucleolus 0.000582 0.001226 0.000129 0.000268 0.000590 0.000383 0.000165 0.000593 0.000586 0.000564 0.000141 0.000283
Nucleus 0.176986* 0.077704 0.286733* 0.173551* 0.121861 0.097270 0.247183* 0.213507* 0.149941* 0.093076 0.273256* 0.175450*
Peroxisomes 0.001720 0.003036 0.000158 0.001087 0.000985 0.003026 0.000133 0.000520 0.001360 0.003028 0.000149 0.001060
Vacuole 0.035701 0.077771 0.011655 0.055364 0.037594 0.050593 0.065589 0.151120 0.036630 0.056419 0.030034 0.059915
Vacuole Periphery 0.000386 0.001037 0.000094 0.002106 0.000305 0.000602 0.000703 0.000233 0.000346 0.000696 0.000302 0.002017

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.69 0.91 -1.48 -0.79 -1.99 -4.14 -6.89 -1.08 -1.04 -0.98 -0.68 -0.54 -2.13 -1.96 -2.06
Bud Neck 4.45 8.12 7.07 2.87 -3.14 -5.76 1.07 1.60 8.97 0.95 -0.18 7.49 5.73 7.71 -3.25
Bud Site -3.52 1.75 -4.44 -0.50 -6.06 -8.45 -3.49 -1.47 0.08 -0.90 -7.41 -0.79 -6.42 -0.96 -5.98
Cell Periphery -4.81 4.25 -5.64 -3.48 -6.69 1.83 -8.73 -3.59 -3.75 -2.41 0.45 -1.78 -6.44 -6.51 -6.09
Cytoplasm 2.10 8.36 13.25 7.63 6.35 -0.64 20.24 8.83 9.09 1.90 -2.16 19.49 19.17 20.84 3.60
Cytoplasmic Foci -10.41 21.97 19.56 23.24 -7.56 5.03 25.35 6.78 4.32 -5.22 -1.41 33.71 29.50 33.12 -7.36
Eisosomes -1.45 16.01 7.62 7.86 -7.16 0.63 2.52 -3.65 -3.74 -3.97 2.16 17.01 5.64 4.31 -5.10
Endoplasmic Reticulum -5.29 -0.57 -5.44 2.08 -5.35 2.82 -9.86 -4.62 -5.28 -1.66 0.67 -7.08 -5.62 -6.39 0.73
Endosome -6.67 8.84 3.41 7.60 -2.90 -1.77 4.07 -2.11 -1.42 -3.77 -6.53 8.66 4.40 9.75 -2.19
Golgi -0.34 7.45 -0.57 -0.46 -2.13 -3.33 2.10 2.63 4.24 0.23 -2.74 7.32 -1.00 0.30 -2.08
Lipid Particles -3.87 9.72 4.51 6.28 -13.53 1.62 3.56 -2.30 -2.79 -3.03 0.42 10.03 4.32 1.95 -8.44
Mitochondria 4.12 6.04 4.46 -0.08 -3.02 -0.62 1.47 1.67 6.29 4.45 -0.16 3.19 2.44 4.35 -3.03
Mitotic Spindle 0.83 -4.87 -8.54 -9.05 -5.79 -3.53 -2.78 -1.10 0.07 -0.28 -3.87 -5.37 -8.54 -6.13 -6.13
None 1.85 -5.31 -2.98 -3.89 2.64 -0.03 -4.86 2.33 2.89 7.96 2.15 -8.03 -5.47 -7.91 2.04
Nuclear Periphery -2.27 -19.01 -21.95 -8.94 -9.86 -0.04 -21.18 -6.81 -6.37 -1.22 -1.09 -27.34 -22.66 -13.32 -6.87
Nuclear Periphery Foci -8.51 -5.83 -11.54 2.52 -6.91 4.96 -10.76 -5.79 -6.31 -2.72 -1.21 -11.20 -10.89 -8.76 0.21
Nucleolus -1.94 7.97 6.50 2.73 -4.54 0.90 1.81 0.76 -0.22 -2.45 -0.19 3.73 3.03 3.54 -5.06
Nucleus 17.56 -24.65 -5.84 -23.75 19.36 5.76 -23.84 -2.77 -3.99 4.89 16.53 -36.03 -12.36 -26.26 19.40
Peroxisomes -2.05 5.98 4.42 4.22 -1.24 -4.35 3.43 2.28 6.25 -2.94 -4.39 6.67 4.18 6.97 -1.38
Vacuole -9.73 4.09 -22.91 -10.59 -25.25 -5.74 -16.53 -8.52 -7.86 -4.40 -10.51 -10.01 -24.44 -19.14 -18.00
Vacuole Periphery -3.08 8.15 -0.87 1.26 -2.07 -2.78 -0.19 2.43 3.87 0.75 -3.68 2.45 -1.01 0.38 -1.67
ORF
Human Ortholog
Description Putative protein of unknown function; possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YDR391C

YDR391C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr391c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available