Standard name | |
---|---|
Human Ortholog | |
Description | ATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 2 | 3 | 6 | 4 | 6 | 0 | 3 | 7 | 2 | 9 | 22 |
Bud | 4 | 0 | 2 | 5 | 5 | 5 | 17 | 10 | 7 | 13 | 13 | 10 | 0 | 0 | 1 | 4 | 2 | 1 | 0 | 1 | 3 | 5 | 21 | 20 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 8 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 3 | 8 | 5 | 6 | 5 | 4 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 1 | 18 | 3 | 3 | 2 | 3 | 4 | 1 | 3 | 4 | 2 | 13 | 11 | 13 | 17 | 17 | 18 | 0 | 0 | 1 | 0 | 0 | 2 |
Cytoplasm | 5 | 0 | 11 | 16 | 15 | 25 | 28 | 33 | 17 | 25 | 15 | 19 | 35 | 116 | 83 | 13 | 15 | 6 | 1 | 0 | 0 | 8 | 8 | 9 |
Endoplasmic Reticulum | 2 | 1 | 1 | 2 | 1 | 2 | 4 | 2 | 3 | 5 | 3 | 11 | 7 | 3 | 6 | 3 | 6 | 2 | 0 | 0 | 0 | 1 | 0 | 6 |
Endosome | 6 | 0 | 20 | 2 | 10 | 3 | 3 | 16 | 2 | 2 | 1 | 1 | 19 | 28 | 23 | 4 | 4 | 3 | 6 | 4 | 9 | 11 | 15 | 21 |
Golgi | 0 | 2 | 4 | 4 | 7 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 84 | 163 | 145 | 87 | 66 | 48 | 6 | 6 | 13 | 20 | 33 | 31 |
Mitochondria | 168 | 48 | 215 | 183 | 226 | 332 | 546 | 420 | 375 | 453 | 329 | 437 | 117 | 123 | 136 | 14 | 12 | 7 | 157 | 51 | 203 | 211 | 280 | 272 |
Nucleus | 0 | 0 | 1 | 0 | 0 | 7 | 7 | 8 | 5 | 2 | 7 | 2 | 1 | 3 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 1 | 3 | 2 |
Nuclear Periphery | 3 | 0 | 9 | 4 | 8 | 29 | 21 | 8 | 11 | 8 | 10 | 13 | 2 | 2 | 1 | 4 | 3 | 4 | 1 | 0 | 0 | 1 | 3 | 3 |
Nucleolus | 5 | 1 | 4 | 2 | 3 | 13 | 24 | 25 | 7 | 11 | 9 | 7 | 1 | 0 | 1 | 5 | 3 | 5 | 0 | 0 | 0 | 0 | 1 | 1 |
Peroxisomes | 1 | 0 | 26 | 3 | 5 | 5 | 0 | 8 | 1 | 4 | 2 | 4 | 4 | 13 | 8 | 1 | 6 | 3 | 2 | 1 | 21 | 3 | 7 | 6 |
SpindlePole | 4 | 1 | 6 | 1 | 3 | 2 | 12 | 8 | 10 | 14 | 7 | 9 | 2 | 5 | 11 | 0 | 2 | 2 | 2 | 0 | 0 | 5 | 9 | 8 |
Vac/Vac Membrane | 26 | 4 | 16 | 3 | 33 | 22 | 41 | 27 | 34 | 31 | 34 | 35 | 54 | 121 | 53 | 27 | 34 | 32 | 2 | 1 | 0 | 24 | 36 | 36 |
Unique Cell Count | 182 | 52 | 253 | 206 | 259 | 373 | 585 | 478 | 397 | 481 | 348 | 461 | 252 | 442 | 359 | 160 | 151 | 116 | 183 | 73 | 266 | 304 | 442 | 459 |
Labelled Cell Count | 227 | 58 | 334 | 228 | 319 | 451 | 715 | 575 | 480 | 577 | 438 | 552 | 341 | 590 | 485 | 187 | 177 | 139 | 183 | 73 | 266 | 304 | 442 | 459 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.4 | 8.7 | 7.7 | 7.4 | 6.4 | 5.1 | 4.6 | 4.5 | 4.5 | 4.3 | 4.0 | 4.0 | 8.9 | 8.5 | 8.1 | 11.2 | 10.6 | 11.0 | 8.6 | 9.0 | 9.2 |
Std Deviation (1e-4) | 2.0 | 3.1 | 2.5 | 2.9 | 2.1 | 1.9 | 1.6 | 1.4 | 1.5 | 1.4 | 1.4 | 1.3 | 2.5 | 2.5 | 2.3 | 2.2 | 2.2 | 2.4 | 2.5 | 2.5 | 2.7 |
Intensity Change (Log2) | – | – | – | -0.05 | -0.26 | -0.59 | -0.72 | -0.76 | -0.76 | -0.82 | -0.92 | -0.94 | 0.22 | 0.15 | 0.09 | 0.55 | 0.46 | 0.52 | 0.17 | 0.23 | 0.26 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.9 | -3.4 | -4.6 | 0 | 0 | 0 | 0 | 0 | 0 | -0.9 | -2.9 | -1.9 | 1.2 | 1.4 | 2.5 |
Cytoplasm | 1.5 | 0.7 | 1.2 | 0.3 | 1.4 | 0 | 0.5 | 0 | -0.1 | 3.7 | 7.2 | 6.3 | 1.6 | 2.2 | 0.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.5 | -1.9 | -4.6 | 0 | 0 | 0 | 0 | 0 | 0 | -0.2 | -0.8 | -0.7 | -2.3 | -2.2 | -2.0 |
Golgi | 0 | 0.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 9.4 | 10.5 | 11.0 | 12.6 | 10.8 | 10.2 |
Mitochondria | 1.2 | 0.7 | 1.5 | 0 | 0 | 0 | 0 | 0 | 0 | -9.1 | -14.5 | -11.6 | -15.2 | -15.1 | -14.4 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -1.0 | -0.3 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | 0 | 0 | -0.6 | 0 | 0 |
Nucleolus | 0 | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -3.9 | -4.0 | -5.1 | 0 | 0 | 0 | 0 | 0 | 0 | -4.1 | -4.0 | -4.3 | -3.9 | -2.3 | -2.5 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.5 | 0 | 0 | 0 |
Vacuole | -2.6 | 2.5 | -0.2 | 0.4 | -0.4 | 1.0 | 0.1 | 1.5 | 0.6 | 4.9 | 6.7 | 3.3 | 3.4 | 4.8 | 5.6 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.7238 | 1.9764 | 1.7081 | 0.9181 | 0.9861 | 1.779 | 1.5383 | 0.947 | 1.2111 | 0.9184 | 1.8297 | 0.9264 | 0.563 | 1.3955 | 1.1867 | 1.3967 | 0.7423 | 0.9347 |
Actin | 0.0509 | 0.0253 | 0.0219 | 0.0467 | 0.0245 | 0.0059 | 0.0901 | 0.0088 | 0.0487 | 0.0397 | 0.0061 | 0.013 | 0.0573 | 0.0181 | 0.0106 | 0.0446 | 0.0199 | 0.0139 |
Bud | 0.003 | 0.0067 | 0.008 | 0.0068 | 0.0033 | 0.0015 | 0.0025 | 0.0064 | 0.0056 | 0.0041 | 0.0052 | 0.0017 | 0.0032 | 0.0231 | 0.0102 | 0.0016 | 0.0438 | 0.0082 |
Bud Neck | 0.0047 | 0.0025 | 0.0055 | 0.0013 | 0.0066 | 0.0061 | 0.0085 | 0.0009 | 0.0015 | 0.003 | 0.029 | 0.0068 | 0.0061 | 0.0016 | 0.0058 | 0.0007 | 0.0006 | 0.0052 |
Bud Periphery | 0.0079 | 0.0126 | 0.0222 | 0.0285 | 0.0043 | 0.0041 | 0.0028 | 0.0078 | 0.0169 | 0.0068 | 0.0159 | 0.0054 | 0.0084 | 0.0339 | 0.0217 | 0.0041 | 0.059 | 0.0196 |
Bud Site | 0.0238 | 0.0803 | 0.0368 | 0.0053 | 0.0063 | 0.002 | 0.022 | 0.033 | 0.0085 | 0.0099 | 0.0264 | 0.0011 | 0.016 | 0.0648 | 0.0284 | 0.0024 | 0.0039 | 0.0024 |
Cell Periphery | 0.008 | 0.0045 | 0.0031 | 0.0025 | 0.0008 | 0.0019 | 0.0009 | 0.0027 | 0.0016 | 0.0016 | 0.0032 | 0.001 | 0.0012 | 0.0023 | 0.0017 | 0.0014 | 0.0012 | 0.0014 |
Cytoplasm | 0.0684 | 0.1029 | 0.1107 | 0.1538 | 0.1354 | 0.1096 | 0.0395 | 0.0711 | 0.0694 | 0.0752 | 0.0087 | 0.0847 | 0.0587 | 0.081 | 0.078 | 0.0399 | 0.0323 | 0.0605 |
Cytoplasmic Foci | 0.076 | 0.0849 | 0.0466 | 0.0849 | 0.0566 | 0.0708 | 0.1001 | 0.0794 | 0.0659 | 0.0752 | 0.065 | 0.0798 | 0.0932 | 0.0478 | 0.0586 | 0.0498 | 0.075 | 0.051 |
Eisosomes | 0.0018 | 0.0009 | 0.001 | 0.0007 | 0.0002 | 0.0004 | 0.001 | 0.0018 | 0.0008 | 0.0008 | 0.0001 | 0.0003 | 0.0008 | 0.001 | 0.0004 | 0.0008 | 0.0003 | 0.0003 |
Endoplasmic Reticulum | 0.0124 | 0.0074 | 0.0109 | 0.004 | 0.0016 | 0.0034 | 0.006 | 0.0042 | 0.0053 | 0.0056 | 0.0004 | 0.0038 | 0.0046 | 0.0031 | 0.0043 | 0.0029 | 0.001 | 0.0064 |
Endosome | 0.0803 | 0.0819 | 0.0665 | 0.1083 | 0.0616 | 0.1091 | 0.1257 | 0.0888 | 0.0834 | 0.1224 | 0.09 | 0.1162 | 0.1273 | 0.0756 | 0.0882 | 0.0553 | 0.0827 | 0.1015 |
Golgi | 0.0362 | 0.0518 | 0.0276 | 0.0192 | 0.0154 | 0.0658 | 0.071 | 0.0644 | 0.0499 | 0.0908 | 0.0406 | 0.0644 | 0.0651 | 0.0434 | 0.0418 | 0.0433 | 0.0258 | 0.0462 |
Lipid Particles | 0.0437 | 0.0113 | 0.0083 | 0.0095 | 0.0023 | 0.026 | 0.0741 | 0.0068 | 0.0195 | 0.015 | 0.0063 | 0.0224 | 0.0333 | 0.0096 | 0.0155 | 0.0225 | 0.0028 | 0.0106 |
Mitochondria | 0.3288 | 0.2778 | 0.4008 | 0.2753 | 0.4904 | 0.4225 | 0.2358 | 0.4019 | 0.4352 | 0.4343 | 0.5583 | 0.4036 | 0.3386 | 0.4188 | 0.452 | 0.4084 | 0.4818 | 0.5188 |
None | 0.1576 | 0.1695 | 0.1784 | 0.1753 | 0.1569 | 0.1403 | 0.103 | 0.1806 | 0.1326 | 0.0624 | 0.0998 | 0.1379 | 0.1444 | 0.1185 | 0.1364 | 0.2548 | 0.1211 | 0.0926 |
Nuclear Periphery | 0.0086 | 0.0014 | 0.0068 | 0.0017 | 0.0005 | 0.0006 | 0.0127 | 0.0012 | 0.0027 | 0.0022 | 0.0004 | 0.0031 | 0.0034 | 0.001 | 0.0013 | 0.0006 | 0.0003 | 0.0094 |
Nucleolus | 0.0011 | 0.0017 | 0.0018 | 0.0023 | 0.0006 | 0.0004 | 0.0012 | 0.0008 | 0.0007 | 0.0009 | 0.002 | 0.0003 | 0.0009 | 0.0036 | 0.0011 | 0.0005 | 0.0017 | 0.0004 |
Nucleus | 0.0151 | 0.0025 | 0.0034 | 0.0027 | 0.001 | 0.001 | 0.0122 | 0.0013 | 0.0018 | 0.0019 | 0.0023 | 0.0008 | 0.0015 | 0.0048 | 0.0016 | 0.0006 | 0.0009 | 0.0014 |
Peroxisomes | 0.0311 | 0.0337 | 0.0079 | 0.0119 | 0.0172 | 0.0133 | 0.0563 | 0.0218 | 0.0354 | 0.0285 | 0.0211 | 0.0273 | 0.0215 | 0.023 | 0.0209 | 0.0597 | 0.0361 | 0.034 |
Punctate Nuclear | 0.0031 | 0.0018 | 0.0036 | 0.0058 | 0.0006 | 0.0008 | 0.0091 | 0.0009 | 0.0012 | 0.0011 | 0.0024 | 0.0013 | 0.002 | 0.0018 | 0.0012 | 0.0009 | 0.0016 | 0.0011 |
Vacuole | 0.0289 | 0.0338 | 0.0173 | 0.0444 | 0.009 | 0.0102 | 0.0187 | 0.0103 | 0.0091 | 0.0126 | 0.0146 | 0.0185 | 0.0084 | 0.0171 | 0.0149 | 0.0029 | 0.0049 | 0.0081 |
Vacuole Periphery | 0.0085 | 0.0051 | 0.011 | 0.0091 | 0.0049 | 0.0045 | 0.0065 | 0.005 | 0.0041 | 0.0061 | 0.0021 | 0.0069 | 0.004 | 0.0062 | 0.0053 | 0.0023 | 0.003 | 0.007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 83.5912 | 67.4445 | 87.5818 | 100.0554 | 56.6909 | 71.9772 | 96.0903 | 93.6205 | 93.3701 | 83.1394 |
Translational Efficiency | 1.5631 | 1.8167 | 1.5176 | 1.3786 | 1.8423 | 2.049 | 1.5428 | 1.4626 | 1.6286 | 1.45 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1691 | 1344 | 897 | 1316 | 1904 | 2138 | 862 | 1206 | 3595 | 3482 | 1759 | 2522 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 800.94 | 853.72 | 1085.16 | 1031.31 | 821.86 | 857.28 | 1164.22 | 1041.91 | 812.02 | 855.91 | 1123.90 | 1036.38 |
Standard Deviation | 142.73 | 172.35 | 206.73 | 212.40 | 160.58 | 169.73 | 205.83 | 200.24 | 152.80 | 170.76 | 210.04 | 206.74 |
Intensity Change Log 2 | — | 0.092069 | 0.438142 | 0.364712 | — | 0.060874 | 0.502399 | 0.342266 | — | 0.076355 | 0.471042 | 0.353388 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000862 | 0.001613 | 0.004105 | 0.022304 | 0.001080 | 0.001459 | 0.004764 | 0.008591 | 0.000978 | 0.001519 | 0.004428 | 0.015747 |
Bud Neck | 0.013671 | 0.025636 | 0.008797 | 0.006831 | 0.010574 | 0.013303 | 0.007211 | 0.007331 | 0.012031 | 0.018063 | 0.008020 | 0.007070 |
Bud Site | 0.046576 | 0.064254 | 0.042987 | 0.150604* | 0.045300 | 0.055287 | 0.042307 | 0.112760* | 0.045900 | 0.058748 | 0.042653 | 0.132507* |
Cell Periphery | 0.001462 | 0.000945 | 0.000400 | 0.002260 | 0.001464 | 0.000945 | 0.000609 | 0.001507 | 0.001463 | 0.000945 | 0.000502 | 0.001900 |
Cytoplasm | 0.036608 | 0.062394 | 0.027824 | 0.039341 | 0.041624 | 0.049389 | 0.018889 | 0.046153 | 0.039265 | 0.054409 | 0.023446 | 0.042599 |
Cytoplasmic Foci | 0.041919 | 0.085626 | 0.037732 | 0.025069 | 0.047428 | 0.054535 | 0.034302 | 0.037398 | 0.044836 | 0.066536 | 0.036051 | 0.030965 |
Eisosomes | 0.000097 | 0.000114 | 0.000080 | 0.000183 | 0.000090 | 0.000120 | 0.000089 | 0.000119 | 0.000093 | 0.000117 | 0.000085 | 0.000152 |
Endoplasmic Reticulum | 0.000965 | 0.001001 | 0.002028 | 0.002113 | 0.001066 | 0.000752 | 0.002146 | 0.001877 | 0.001018 | 0.000848 | 0.002086 | 0.002001 |
Endosome | 0.044932 | 0.081057 | 0.045629 | 0.022555 | 0.047303 | 0.065784 | 0.038885 | 0.042826 | 0.046187 | 0.071679 | 0.042324 | 0.032248 |
Golgi | 0.038435 | 0.066038 | 0.054315 | 0.061269 | 0.040578 | 0.057434 | 0.051816 | 0.082741 | 0.039570 | 0.060755 | 0.053090 | 0.071537 |
Lipid Particles | 0.003141 | 0.004110 | 0.002897 | 0.000564 | 0.004202 | 0.003141 | 0.001439 | 0.001215 | 0.003703 | 0.003515 | 0.002183 | 0.000875 |
Mitochondria | 0.687457* | 0.454339* | 0.542768* | 0.554482* | 0.666797* | 0.574006* | 0.607386* | 0.483067* | 0.676515* | 0.527816* | 0.574434* | 0.520332* |
Mitotic Spindle | 0.011367 | 0.031270 | 0.087239 | 0.032315 | 0.013370 | 0.027127 | 0.073517 | 0.048569 | 0.012428 | 0.028726 | 0.080515 | 0.040088 |
None | 0.001966 | 0.001821 | 0.001008 | 0.000675 | 0.001871 | 0.001331 | 0.000805 | 0.001718 | 0.001916 | 0.001521 | 0.000908 | 0.001174 |
Nuclear Periphery | 0.000149 | 0.000254 | 0.001710 | 0.000148 | 0.000235 | 0.000330 | 0.001931 | 0.000402 | 0.000195 | 0.000301 | 0.001818 | 0.000269 |
Nuclear Periphery Foci | 0.000239 | 0.000749 | 0.001327 | 0.000245 | 0.000505 | 0.000435 | 0.001410 | 0.000835 | 0.000380 | 0.000556 | 0.001368 | 0.000527 |
Nucleolus | 0.000627 | 0.001629 | 0.001356 | 0.000105 | 0.000695 | 0.001174 | 0.000818 | 0.000383 | 0.000663 | 0.001350 | 0.001092 | 0.000238 |
Nucleus | 0.004753 | 0.011255 | 0.018258 | 0.004659 | 0.004724 | 0.005435 | 0.012539 | 0.004405 | 0.004738 | 0.007681 | 0.015455 | 0.004538 |
Peroxisomes | 0.018199 | 0.032596 | 0.018197 | 0.022865 | 0.021386 | 0.025089 | 0.023551 | 0.021656 | 0.019887 | 0.027987 | 0.020821 | 0.022287 |
Vacuole | 0.025430 | 0.052007 | 0.057617 | 0.034065 | 0.027298 | 0.040861 | 0.040356 | 0.058631 | 0.026419 | 0.045163 | 0.049158 | 0.045812 |
Vacuole Periphery | 0.021147 | 0.021290 | 0.043727 | 0.017346 | 0.022408 | 0.022066 | 0.035231 | 0.037817 | 0.021815 | 0.021766 | 0.039564 | 0.027135 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.63 | -3.43 | -9.48 | -9.10 | -7.38 | -2.09 | -4.96 | -6.47 | -6.09 | -2.67 | -3.44 | -5.72 | -11.26 | -10.80 | -7.75 |
Bud Neck | -4.72 | 2.75 | 4.15 | 8.12 | 1.56 | -2.10 | 2.44 | 1.98 | 4.20 | -0.32 | -4.69 | 3.59 | 4.43 | 9.08 | 0.90 |
Bud Site | -3.59 | 0.58 | -14.34 | -11.48 | -14.29 | -2.45 | 0.45 | -10.55 | -8.96 | -10.10 | -4.10 | 0.72 | -17.78 | -14.90 | -17.39 |
Cell Periphery | 2.99 | 7.59 | -2.19 | -3.88 | -5.87 | 2.53 | 4.34 | 0.03 | -2.02 | -3.45 | 3.81 | 7.83 | -1.67 | -4.32 | -6.74 |
Cytoplasm | -5.48 | 1.24 | -2.44 | 3.12 | -3.27 | -1.94 | 4.37 | -2.77 | -1.14 | -6.39 | -5.06 | 3.87 | -3.60 | 1.16 | -6.77 |
Cytoplasmic Foci | -9.70 | 1.18 | 5.38 | 14.56 | 3.93 | -2.06 | 3.64 | 2.81 | 4.97 | -1.01 | -8.04 | 3.45 | 5.91 | 13.98 | 1.99 |
Eisosomes | -2.44 | 1.86 | -7.90 | -6.53 | -9.45 | -1.51 | -0.08 | -2.53 | 0.07 | -2.32 | -1.98 | 1.23 | -7.58 | -2.54 | -8.24 |
Endoplasmic Reticulum | -0.28 | -4.98 | -4.06 | -3.99 | 0.54 | 2.44 | -3.19 | -3.33 | -4.74 | 0.89 | 1.75 | -5.45 | -5.22 | -6.30 | 1.02 |
Endosome | -8.90 | -0.02 | 8.58 | 16.79 | 7.95 | -5.64 | 2.72 | 1.82 | 7.30 | -0.92 | -10.04 | 1.85 | 7.16 | 17.24 | 4.73 |
Golgi | -6.84 | -3.36 | -4.85 | 1.40 | -1.17 | -5.19 | -2.36 | -8.08 | -4.45 | -5.14 | -8.41 | -4.04 | -9.15 | -2.38 | -4.48 |
Lipid Particles | -1.43 | 0.05 | 4.58 | 6.29 | 5.28 | 1.58 | 4.38 | 4.68 | 4.05 | 0.92 | 0.29 | 2.91 | 6.63 | 7.56 | 4.94 |
Mitochondria | 17.17 | 10.18 | 10.99 | -5.26 | -0.23 | 7.82 | 4.82 | 14.25 | 7.75 | 7.37 | 16.66 | 10.60 | 17.61 | 2.57 | 4.89 |
Mitotic Spindle | -7.39 | -11.94 | -7.96 | -0.39 | 8.05 | -6.44 | -10.74 | -9.26 | -5.37 | 3.77 | -9.79 | -16.04 | -12.10 | -4.51 | 8.43 |
None | 0.37 | 2.79 | 4.53 | 4.84 | 2.36 | 1.71 | 4.14 | 0.26 | -0.36 | -1.02 | 1.60 | 4.73 | 1.84 | 0.99 | -0.48 |
Nuclear Periphery | -2.65 | -5.62 | -0.65 | 1.93 | 5.53 | -0.72 | -2.28 | -1.87 | -0.66 | 2.00 | -1.33 | -4.19 | -1.87 | 0.06 | 3.91 |
Nuclear Periphery Foci | -1.49 | -5.08 | -1.51 | 1.26 | 4.66 | 0.43 | -1.97 | -0.89 | -1.15 | 0.99 | -1.11 | -3.97 | -1.02 | -0.06 | 2.73 |
Nucleolus | -3.81 | -1.93 | 4.36 | 6.28 | 3.16 | -1.69 | -0.87 | 2.36 | 3.12 | 2.83 | -3.52 | -2.10 | 4.71 | 6.21 | 4.00 |
Nucleus | -4.15 | -5.57 | -1.89 | 2.14 | 4.72 | -0.80 | -3.78 | -1.58 | -0.79 | 3.07 | -3.36 | -6.71 | -2.48 | 0.65 | 5.59 |
Peroxisomes | -5.08 | 0.08 | -1.58 | 3.19 | -1.51 | -1.62 | -0.82 | -0.09 | 1.25 | 0.67 | -4.60 | -0.51 | -1.14 | 2.88 | -0.50 |
Vacuole | -8.54 | -9.14 | -5.92 | 1.09 | 2.79 | -5.59 | -4.70 | -9.60 | -6.35 | -4.66 | -9.94 | -9.91 | -10.97 | -4.07 | -1.22 |
Vacuole Periphery | -0.00 | -6.46 | 2.33 | 2.35 | 7.75 | 0.21 | -4.01 | -4.75 | -5.09 | -0.53 | 0.09 | -7.48 | -2.48 | -2.65 | 5.01 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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