Standard name
Human Ortholog
Description Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0.05 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 – 0 0 0 0 0.07 0.11 0.13 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0.06 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.06 0.09 0 0 0 0.07 0.05 – 0.1 0.06 0.15 0.06 0 0 0 0.12 0.08 0.12 0 0 0 0 0 0
Nucleus 0.22 0.14 0.2 0.11 0.08 0 0.06 – 0.05 0.08 0.09 0.05 0.07 0 0 0.12 0.11 0.15 0.09 0.11 0.13 0.09 0.13 0.09
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.96 0.91 0.85 0.89 0.89 0.91 0.88 – 0.87 0.83 0.88 0.91 0.85 0.84 0.79 0.87 0.84 0.83 0.87 0.81 0.75 0.83 0.72 0.75
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.06 0 0.14 0.08 0.12 0.21 0.21 – 0.31 0.3 0.18 0.35 0.09 0.09 0.11 0 0 0 0 0 0.05 0 0.07 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 2 0 0 0 0 0 – 2 0 3 0 0 0 0 1 0 0 0 1 2 1 2 1
Bud Neck 2 2 1 3 0 3 5 – 1 5 5 6 5 3 3 0 0 0 0 2 1 0 0 1
Bud Site 0 1 0 1 1 1 3 – 9 6 2 8 1 1 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 5 5 4 10 8 11 – 1 6 5 1 14 26 30 10 10 10 0 3 0 0 1 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 3 5 1 0 0 0 0 0 1
Endosome 0 0 2 4 8 8 17 – 8 14 2 8 3 4 9 1 4 1 0 3 2 2 4 5
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 6 36 1 6 5 15 15 – 19 14 28 11 1 4 2 30 20 26 0 1 1 0 0 2
Nucleus 21 57 33 16 17 5 16 – 9 20 17 10 13 8 8 31 27 31 8 45 22 15 27 19
Nuclear Periphery 0 2 0 0 0 0 1 – 1 2 0 0 0 1 0 0 0 0 0 1 0 0 1 0
Nucleolus 90 364 139 136 201 181 244 – 173 210 170 171 169 197 185 222 199 176 80 338 129 145 150 173
Peroxisomes 0 4 1 0 1 6 5 – 2 1 3 4 0 1 5 0 2 1 0 0 0 0 0 0
SpindlePole 6 1 23 12 27 42 58 – 61 75 35 65 18 20 26 3 2 6 2 12 7 4 13 12
Vac/Vac Membrane 0 0 0 0 3 0 0 – 0 1 1 0 1 0 2 3 6 5 0 4 1 1 4 6
Unique Cell Count 94 398 164 152 226 200 276 199 254 193 187 198 234 234 256 236 213 92 418 172 175 209 232
Labelled Cell Count 126 475 206 182 273 269 375 286 354 272 284 225 265 270 304 276 258 92 418 172 175 209 232


Nucleolus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 17.5 23.0 17.8 16.3 15.0 12.0 11.2 – 9.5 10.3 9.1 9.4 19.0 17.5 16.4 27.7 28.2 26.0 16.6 16.6 16.7
Std Deviation (1e-4) 5.2 6.8 6.0 4.8 4.9 4.1 2.7 – 3.2 3.5 2.9 3.1 8.4 6.8 7.3 12.9 11.5 11.8 5.0 5.4 5.4
Intensity Change (Log2) – – – -0.12 -0.24 -0.57 -0.67 – -0.91 -0.78 -0.97 -0.92 0.09 -0.03 -0.11 0.64 0.67 0.55 -0.09 -0.1 -0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.7 0.5 0.5 – 0 0 0 0 1.7 3.0 3.4 0.5 0.6 0.8
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 2.5 – 0 2.2 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 3.2 2.6 – 3.7 2.6 4.8 2.7 0 0 0 4.2 3.5 4.3
Nucleus -2.4 -3.7 -5.5 -4.6 – -4.6 -3.7 -3.1 -4.2 -3.9 -5.4 -5.4 -2.2 -2.4 -1.4
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.2 1.2 1.7 1.1 – 0.6 -0.6 0.9 1.9 0.2 -0.2 -1.4 0.6 -0.1 -0.6
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole -1.7 -0.6 1.7 1.8 – 3.7 3.7 1.0 4.5 -1.5 -1.7 -0.9 -5.3 -5.4 -4.0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.278 13.1794 12.6863 11.6057 12.8058 11.3404 12.2559 15.0037 13.0881 11.8826 10.3793 12.9066 14.4916 15.3324 14.2936 12.9077 12.3184 13.082
Actin 0.0104 0 0 0.0058 0 0.0004 0.0019 0 0.0006 0 0.0017 0.001 0.0147 0.0001 0.011 0.0095 0.0049 0
Bud 0.0003 0 0 0.0001 0.0001 0 0.0001 0 0 0 0.0002 0 0.0001 0 0.0001 0.0013 0.0001 0
Bud Neck 0.0004 0.0001 0 0.0003 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002 0.0002 0.0001 0.0006 0.0001 0.0001 0.0027 0.0006 0.0001
Bud Periphery 0.0004 0 0 0.0002 0.0001 0.0001 0.0002 0 0 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0011 0.0002 0
Bud Site 0.0015 0 0 0.0002 0.0002 0.0001 0.0005 0 0.0002 0.0003 0.0007 0 0.0007 0.0001 0.0001 0.0034 0.0002 0
Cell Periphery 0.0004 0.0001 0.0001 0.0004 0.0005 0.0003 0.0001 0.0002 0.0001 0.0002 0.0004 0 0.0001 0.0001 0.0001 0.0009 0.0003 0.0001
Cytoplasm 0.0036 0 0 0.0001 0 0.0001 0.0011 0 0.001 0.0002 0.0026 0 0.0006 0 0 0.0012 0.0003 0
Cytoplasmic Foci 0.0036 0.0001 0.0006 0.0023 0.0004 0.0082 0.0086 0 0.0077 0.0016 0.0239 0.0003 0.0068 0 0.001 0.0245 0.0004 0.0001
Eisosomes 0.0002 0 0 0.0002 0.0001 0 0.0001 0 0 0 0.0002 0 0.0001 0 0 0.0002 0.0002 0
Endoplasmic Reticulum 0.0081 0 0 0.0003 0 0 0.0008 0 0.0003 0.0001 0.0041 0.0004 0.0007 0 0.0001 0.0008 0.0022 0
Endosome 0.0111 0 0 0.0045 0 0.0008 0.0101 0 0.0042 0.0004 0.0298 0.0018 0.0041 0 0.0001 0.0042 0.0082 0
Golgi 0.0028 0 0 0.006 0 0.0011 0.0013 0 0.0005 0.0001 0.0076 0.0006 0.0019 0 0.0003 0.0025 0.0014 0
Lipid Particles 0.0105 0.0002 0.0005 0.0564 0.0017 0.0041 0.0185 0.0001 0.0046 0.0019 0.0294 0.0006 0.0044 0.0001 0.0005 0.0119 0.0018 0.0002
Mitochondria 0.0152 0.0001 0.0016 0.0266 0.0417 0.0318 0.0034 0.0001 0.001 0.0003 0.0341 0.0032 0.0007 0.001 0.0005 0.0068 0.0061 0.0001
None 0.0008 0 0 0.0006 0 0.0002 0.0013 0 0.0006 0.0001 0.0014 0.0001 0.002 0 0 0.0037 0.0012 0
Nuclear Periphery 0.0015 0 0 0.0043 0 0 0.0191 0 0.0033 0.0019 0.0078 0.0052 0.0013 0.0003 0.0001 0.0037 0.0399 0
Nucleolus 0.8681 0.9287 0.9296 0.8081 0.8765 0.9021 0.8256 0.8877 0.8756 0.866 0.7715 0.9196 0.8699 0.8781 0.9061 0.839 0.8667 0.9292
Nucleus 0.0431 0.0703 0.0668 0.0747 0.0746 0.0476 0.0784 0.1107 0.0894 0.1163 0.0731 0.0648 0.0815 0.1188 0.0793 0.0508 0.0581 0.0696
Peroxisomes 0.0019 0 0.0001 0.0053 0.0018 0.0007 0.0018 0 0.002 0 0.0043 0.0001 0.0059 0 0.0001 0.0056 0.0003 0
Punctate Nuclear 0.0015 0.0001 0.0002 0.0012 0.0012 0.0012 0.0246 0.0001 0.0071 0.0074 0.0016 0.0011 0.0032 0.0005 0.0002 0.0245 0.0029 0.0005
Vacuole 0.012 0.0002 0.0002 0.0009 0.0004 0.0005 0.001 0.0007 0.0005 0.0024 0.0023 0.0002 0.0003 0.0004 0.0001 0.0012 0.0012 0.0001
Vacuole Periphery 0.0025 0.0001 0.0001 0.0018 0.0003 0.0004 0.0013 0.0003 0.001 0.0007 0.003 0.0007 0.0002 0.0002 0.0001 0.0007 0.0027 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.8329 36.7594 40.5367 39.5088 43.3182 29.111 30.7147 25.9565 24.7339 44.3916
Translational Efficiency 1.9828 1.6644 1.5487 1.3482 1.4373 2.0231 1.2965 1.1092 1.2047 1.1992

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2222 1308 328 1199 541 2162 2675 43 2763 3470 3003 1242

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1112.10 1391.62 1884.06 2103.07 1542.93 1435.71 1870.03 2132.97 1196.46 1419.09 1871.56 2104.11
Standard Deviation 234.36 391.04 327.67 556.52 333.03 331.59 374.17 676.84 308.40 355.81 369.40 561.15
Intensity Change Log 2 — 0.323479 0.760558 0.919210 — -0.103908 0.277389 0.467191 — 0.090712 0.499735 0.673988

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000255 0.000303 0.000258 0.000517 0.000157 0.000292 0.000334 0.000488 0.000236 0.000296 0.000326 0.000516
Bud Neck 0.001386 0.001168 0.002140 0.006353 0.000756 0.001856 0.002067 0.014638 0.001262 0.001597 0.002075 0.006640
Bud Site 0.000712 0.000562 0.000619 0.001548 0.000159 0.000389 0.000373 0.003511 0.000604 0.000455 0.000400 0.001616
Cell Periphery 0.000054 0.000024 0.000010 0.000068 0.000006 0.000118 0.000023 0.000107 0.000045 0.000083 0.000021 0.000070
Cytoplasm 0.000885 0.000212 0.000042 0.000352 0.000015 0.000188 0.000043 0.000384 0.000715 0.000197 0.000042 0.000353
Cytoplasmic Foci 0.003728 0.004008 0.003813 0.005362 0.002217 0.001695 0.001336 0.011216 0.003432 0.002567 0.001607 0.005565
Eisosomes 0.000395 0.000306 0.000185 0.000236 0.000259 0.000392 0.000312 0.000157 0.000369 0.000360 0.000299 0.000233
Endoplasmic Reticulum 0.000276 0.000260 0.000084 0.000157 0.000112 0.000198 0.000106 0.000211 0.000244 0.000221 0.000104 0.000159
Endosome 0.000135 0.000229 0.000015 0.000701 0.000013 0.000225 0.000019 0.010196 0.000111 0.000226 0.000018 0.001030
Golgi 0.000202 0.000335 0.000361 0.000933 0.000126 0.000192 0.000133 0.015258 0.000187 0.000246 0.000158 0.001429
Lipid Particles 0.000087 0.000188 0.000014 0.000050 0.000013 0.000135 0.000009 0.003848 0.000073 0.000155 0.000009 0.000181
Mitochondria 0.000951 0.001004 0.001169 0.002088 0.000558 0.001003 0.001316 0.006322 0.000874 0.001003 0.001300 0.002235
Mitotic Spindle 0.002291 0.000583 0.017691 0.006512 0.001930 0.002203 0.004368 0.010630 0.002220 0.001593 0.005824 0.006655
None 0.000669 0.000624 0.000240 0.001124 0.000261 0.000542 0.000285 0.001147 0.000589 0.000573 0.000280 0.001125
Nuclear Periphery 0.000092 0.000048 0.000075 0.000268 0.000059 0.000126 0.000132 0.000458 0.000086 0.000097 0.000126 0.000275
Nuclear Periphery Foci 0.000764 0.000359 0.000058 0.000158 0.000235 0.000323 0.000064 0.000223 0.000660 0.000337 0.000063 0.000160
Nucleolus 0.670495* 0.637893* 0.660683* 0.689473* 0.710067* 0.627061* 0.675447* 0.547206* 0.678243* 0.631144* 0.673835* 0.684547*
Nucleus 0.242636* 0.275762* 0.144571* 0.247518* 0.181788* 0.314689* 0.253934* 0.320712* 0.230722* 0.300016* 0.241989* 0.250052*
Peroxisomes 0.072813 0.074674 0.167017* 0.032165 0.100549* 0.046593 0.058688 0.049788 0.078244 0.057178 0.070520 0.032775
Vacuole 0.000653 0.000780 0.000367 0.001778 0.000337 0.000872 0.000319 0.001775 0.000591 0.000837 0.000325 0.001778
Vacuole Periphery 0.000520 0.000677 0.000585 0.002640 0.000382 0.000907 0.000692 0.001727 0.000493 0.000821 0.000680 0.002609

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.89 -0.83 -4.35 -3.50 -3.92 -7.85 -15.19 -2.87 -2.22 -2.01 -3.32 -5.86 -4.96 -3.98 -3.49
Bud Neck 1.59 -2.50 -9.38 -9.62 -5.74 -8.51 -12.50 -2.40 -2.30 -2.29 -3.52 -7.78 -9.21 -8.70 -7.94
Bud Site 0.70 0.11 -2.99 -3.55 -3.38 -5.33 -7.82 -2.59 -2.51 -2.52 0.95 1.28 -3.87 -4.58 -4.76
Cell Periphery 1.99 2.95 -1.26 -5.17 -6.93 -2.05 -3.17 -3.80 -0.37 -3.30 -1.10 1.82 -2.29 0.20 -5.53
Cytoplasm 1.48 1.86 1.00 -1.29 -2.68 -2.38 -3.50 -2.30 -1.55 -2.23 1.23 1.87 0.73 -1.14 -3.04
Cytoplasmic Foci -0.29 -0.46 -2.22 -1.72 -1.89 1.53 3.29 -2.14 -2.28 -2.38 1.96 4.87 -2.85 -3.94 -5.25
Eisosomes 3.85 11.04 6.84 3.16 -2.56 -4.81 -2.12 3.23 7.55 5.47 0.50 3.85 6.46 6.30 3.40
Endoplasmic Reticulum 0.17 4.75 2.63 1.61 -4.06 -3.83 0.27 -1.56 -1.26 -1.58 0.51 4.50 1.73 1.34 -3.91
Endosome -0.67 3.43 -1.64 -1.26 -2.01 -2.00 -1.72 -1.04 -1.01 -1.04 -1.31 3.33 -1.98 -1.67 -2.21
Golgi -1.07 -4.84 -1.72 -1.34 -1.29 -1.25 -0.96 -1.05 -1.05 -1.05 -0.97 1.18 -1.99 -1.88 -2.04
Lipid Particles -0.98 2.51 1.10 1.37 -3.16 -1.61 1.32 -1.05 -1.00 -1.05 -1.26 2.74 -0.81 -0.02 -1.46
Mitochondria -0.28 -1.64 -5.00 -5.39 -4.48 -5.70 -9.22 -1.71 -1.57 -1.46 -0.97 -3.16 -5.79 -5.83 -4.38
Mitotic Spindle 3.61 -4.21 -3.34 -4.45 2.51 -0.42 -2.66 -1.31 -1.28 -1.15 0.96 -4.86 -3.63 -3.94 -1.41
None 0.56 6.44 -0.89 -0.98 -1.71 -3.77 -0.88 -3.83 -2.66 -3.71 0.16 5.35 -1.09 -1.12 -1.72
Nuclear Periphery 3.33 0.31 -3.10 -3.85 -3.18 -2.12 -2.33 -2.86 -2.56 -2.58 -0.86 -2.13 -3.57 -3.25 -2.85
Nuclear Periphery Foci 1.51 2.90 2.47 2.07 -3.99 -1.01 1.84 -0.71 0.63 -2.56 0.85 3.04 2.49 1.75 -4.12
Nucleolus 4.24 1.38 -1.26 -4.49 -2.10 9.09 4.05 3.95 2.27 3.26 8.36 0.97 0.25 -5.25 -0.38
Nucleus -4.25 9.22 -0.92 2.63 -8.83 -13.99 -7.99 -3.08 -0.27 -1.57 -12.00 -1.94 -2.71 5.65 -1.34
Peroxisomes -0.65 -11.07 17.09 15.94 15.75 11.93 9.12 5.71 0.34 1.70 10.03 2.87 20.15 12.97 17.17
Vacuole -0.64 2.73 -5.01 -3.99 -6.66 -2.51 0.29 -1.58 -1.38 -1.59 -1.71 3.29 -5.48 -3.48 -6.81
Vacuole Periphery -2.76 -1.25 -9.34 -8.65 -9.09 -2.84 -10.08 -4.32 -2.28 -3.36 -2.72 -4.81 -9.69 -7.20 -8.89
Standard name
Human Ortholog
Description Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
Localization
Cell Percentages nucleolus (96%)
Cell Cycle Regulation No
Subcompartmental Group nucleolus-2

Utp5

Utp5


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Utp5-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available