Standard name
Human Ortholog
Description Dimeric hypoxanthine-guanine phosphoribosyltransferase; catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.99 0.99 0.96 0.93 0.91 – 0.9 0.87 0.9 0.89 0.99 0.99 0.99 0.99 0.99 1.0 0.97 0.95 0.97 0.96 0.97 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 – 0.09 0.08 0.06 0.06 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 3 2 – 4 2 7 7 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 1 0 – 0 1 0 0 0 0 1 0 0 0 1 5 3 1 0 4
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 7 2 4 10 19 – 16 24 16 29 1 2 2 4 5 1 0 0 0 0 0 0
Cytoplasm 510 542 600 448 540 557 662 – 702 575 498 663 487 651 605 261 223 189 476 515 564 316 447 493
Endoplasmic Reticulum 1 1 1 2 16 16 43 – 68 51 32 48 3 4 7 14 6 10 1 0 1 0 1 4
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 1
Golgi 0 0 0 0 1 0 1 – 8 7 4 6 0 1 1 0 0 0 0 0 0 0 0 0
Mitochondria 0 1 0 2 0 1 0 – 0 0 2 1 0 0 0 1 0 0 0 0 1 0 0 0
Nucleus 0 0 0 1 0 3 3 – 4 1 2 3 0 0 0 0 2 0 3 7 1 1 3 3
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 3 4 0 8 16 16 – 5 16 3 10 4 1 1 0 1 3 2 4 1 3 3 3
Unique Cell Count 513 548 604 451 561 597 726 783 660 552 742 492 655 614 263 225 189 491 541 584 331 464 519
Labelled Cell Count 515 551 612 455 569 607 746 807 677 564 768 495 659 617 281 238 203 491 541 584 331 464 519


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 69.5 57.6 55.1 52.7 47.4 31.9 38.7 – 38.7 38.3 38.2 39.0 65.4 70.1 68.5 68.9 74.0 70.5 53.5 55.1 52.9
Std Deviation (1e-4) 13.2 12.6 11.2 10.1 9.2 9.1 7.9 – 8.7 8.2 8.8 8.9 19.4 15.1 13.5 20.8 17.6 21.0 11.2 12.2 12.9
Intensity Change (Log2) – – – -0.07 -0.22 -0.79 -0.51 – -0.51 -0.52 -0.53 -0.5 0.25 0.35 0.31 0.32 0.42 0.35 -0.04 -0.0 -0.06


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.8 0.8 1.9 – 1.3 2.8 2.1 3.1 0 0 0 0 0 0
Cytoplasm 0 -3.6 -5.6 -6.7 – -7.5 -8.5 -7.1 -7.6 -0.6 0.1 -1.4 -0.2 -0.3 1.1
Endoplasmic Reticulum 0 3.8 3.7 5.8 – 7.2 6.8 5.7 6.1 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.3 2.7 2.3 – 0 2.5 0 1.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 66.5129 85.6285 77.4728 72.2289 72.8029 79.4795 76.9142 88.5881 87.2654 75.3404 76.2077 87.2226 81.8211 93.1556 88.7557 84.7209 89.9901 87.5976
Actin 0.0078 0 0.0004 0.0147 0.0024 0 0.0012 0.0001 0.0043 0.0005 0.0005 0 0.0011 0.0004 0.0001 0 0 0.0002
Bud 0.0008 0 0.0003 0.0002 0.0005 0 0.0011 0 0.0007 0.0012 0.0008 0 0.0005 0.0002 0 0.0001 0 0.0001
Bud Neck 0.0006 0.0001 0.0002 0.0013 0.0003 0.0002 0.0004 0.0002 0.0003 0.0003 0.0003 0.0005 0.0001 0.0001 0.0001 0.0001 0.0001 0.0003
Bud Periphery 0.0005 0 0.0002 0.0002 0.0004 0 0.0011 0 0.0005 0.0008 0.0004 0 0.0002 0.0001 0 0 0 0
Bud Site 0.0003 0 0.0002 0.002 0.0026 0 0.0003 0 0.0002 0.0004 0.0007 0 0.0001 0.0002 0 0 0 0
Cell Periphery 0.0004 0 0.0003 0.0001 0.001 0 0.0005 0.0002 0.0001 0.0015 0.0003 0.0001 0 0.0001 0 0 0 0
Cytoplasm 0.9084 0.9939 0.9605 0.964 0.9291 0.9912 0.9271 0.9911 0.9627 0.9445 0.9077 0.9814 0.9651 0.9929 0.9903 0.989 0.9867 0.9732
Cytoplasmic Foci 0.0069 0 0.0052 0.0006 0.0022 0.0001 0.0021 0.0001 0.0029 0.0046 0.0091 0.0001 0.0005 0.0001 0.0001 0.0001 0.0001 0.0003
Eisosomes 0.0002 0 0 0.0001 0.0007 0 0 0 0.0001 0.0001 0.0002 0 0.0004 0 0 0 0 0
Endoplasmic Reticulum 0.0018 0.0001 0.0003 0.0001 0.0018 0.0001 0.0022 0.001 0.0007 0.0009 0.0003 0.0006 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001
Endosome 0.0046 0 0.0005 0.0007 0.0016 0 0.0027 0 0.0017 0.0029 0.0005 0 0.0003 0 0 0.0001 0 0.0001
Golgi 0.0022 0 0.0002 0.0012 0.0004 0 0.0005 0 0.0004 0.0002 0.0001 0 0.0001 0 0 0 0 0
Lipid Particles 0.0046 0 0.0009 0.0008 0.0011 0 0.0047 0.0001 0.0018 0.0006 0.0014 0 0.0002 0 0 0 0 0
Mitochondria 0.0049 0 0.0016 0.0004 0.0185 0 0.0067 0 0.0034 0.0051 0.0107 0 0.0004 0.0006 0 0 0 0.0003
None 0.0044 0 0.0049 0.0002 0.0056 0 0.0019 0.0001 0.002 0.0032 0.0102 0.0001 0.0046 0.0001 0.0001 0.0001 0 0.0002
Nuclear Periphery 0.0055 0.0006 0.0025 0.0012 0.0068 0.0009 0.0082 0.0011 0.0046 0.0026 0.0022 0.0018 0.003 0.0005 0.0009 0.0012 0.0015 0.0031
Nucleolus 0.0073 0 0.005 0.0001 0.0021 0 0.0015 0 0.0008 0.0043 0.0219 0 0.0005 0 0 0 0 0.0001
Nucleus 0.0278 0.0051 0.0123 0.009 0.0171 0.0072 0.0276 0.0055 0.0093 0.0159 0.026 0.0147 0.0208 0.0043 0.0082 0.0085 0.0113 0.0209
Peroxisomes 0.0019 0 0.0005 0.0011 0.0004 0 0.0017 0 0.0005 0.0003 0.0007 0 0.0001 0 0 0 0 0
Punctate Nuclear 0.0012 0 0.0019 0.0003 0.0011 0 0.0007 0 0.0015 0.0004 0.0034 0.0001 0.0009 0 0 0.0001 0 0.0003
Vacuole 0.0041 0.0001 0.0015 0.0014 0.0024 0.0002 0.0059 0.0003 0.0008 0.0072 0.0015 0.0004 0.0006 0.0001 0 0.0003 0.0001 0.0003
Vacuole Periphery 0.0038 0 0.0007 0.0003 0.0021 0 0.0019 0 0.0005 0.0027 0.0012 0 0.0001 0.0001 0 0.0001 0 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 168.9899 82.9385 102.207 89.6795 117.0696 109.2859 73.3767 59.126 56.187 109.7499
Translational Efficiency 1.7278 2.1686 1.5235 1.5632 1.6349 2.0947 1.8226 1.5057 1.6288 1.4687

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1756 1307 246 1983 1887 2603 2327 1292 3643 3910 2573 3275

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2746.50 3949.82 3650.88 4052.07 3328.42 3894.83 4712.95 3137.44 3047.92 3913.21 4611.41 3691.24
Standard Deviation 580.58 855.78 621.73 1160.03 635.44 739.52 1075.79 920.31 675.41 780.74 1086.82 1161.36
Intensity Change Log 2 — 0.524193 0.410650 0.561065 — 0.226723 0.501793 -0.085250 — 0.368844 0.461298 0.243028

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000132 0.000061 0.000216 0.000249 0.000068 0.000027 0.000063 0.001751 0.000099 0.000039 0.000078 0.000841
Bud Neck 0.000641 0.000470 0.002057 0.002182 0.000385 0.000222 0.000695 0.002736 0.000509 0.000305 0.000825 0.002400
Bud Site 0.001946 0.001043 0.001404 0.005514 0.000646 0.000459 0.002718 0.006326 0.001272 0.000655 0.002592 0.005834
Cell Periphery 0.000475 0.000312 0.001070 0.000260 0.000222 0.000046 0.000315 0.000464 0.000344 0.000135 0.000387 0.000341
Cytoplasm 0.851270* 0.730449* 0.499163* 0.724799* 0.744853* 0.768926* 0.814554* 0.573288* 0.796148* 0.756064* 0.784400* 0.665027*
Cytoplasmic Foci 0.007264 0.005051 0.021179 0.005552 0.003053 0.001915 0.002859 0.007136 0.005083 0.002963 0.004611 0.006176
Eisosomes 0.000001 0.000001 0.000003 0.000002 0.000000 0.000000 0.000001 0.000005 0.000001 0.000000 0.000001 0.000003
Endoplasmic Reticulum 0.000146 0.000032 0.000040 0.000103 0.000044 0.000018 0.000026 0.000291 0.000093 0.000022 0.000027 0.000178
Endosome 0.000179 0.000065 0.000086 0.001144 0.000047 0.000041 0.000137 0.001714 0.000111 0.000049 0.000132 0.001369
Golgi 0.002354 0.000515 0.000630 0.001814 0.000481 0.000176 0.000453 0.000878 0.001384 0.000290 0.000470 0.001445
Lipid Particles 0.000567 0.000276 0.000886 0.000366 0.000311 0.000066 0.000340 0.001746 0.000435 0.000136 0.000392 0.000911
Mitochondria 0.000232 0.000127 0.000238 0.000299 0.000131 0.000091 0.000138 0.000396 0.000179 0.000103 0.000148 0.000337
Mitotic Spindle 0.001481 0.000456 0.001230 0.007458 0.000563 0.001289 0.001267 0.007509 0.001005 0.001010 0.001264 0.007478
None 0.009560 0.021308 0.009256 0.017352 0.017916 0.020053 0.008805 0.025129 0.013889 0.020473 0.008848 0.020420
Nuclear Periphery 0.000095 0.000093 0.000482 0.000198 0.000240 0.000050 0.000090 0.000468 0.000170 0.000064 0.000128 0.000305
Nuclear Periphery Foci 0.001738 0.000826 0.000937 0.000901 0.001106 0.000258 0.000649 0.002560 0.001411 0.000448 0.000677 0.001555
Nucleolus 0.000127 0.000101 0.000094 0.000433 0.000069 0.000072 0.000136 0.000405 0.000097 0.000082 0.000132 0.000422
Nucleus 0.114685 0.234899* 0.448621* 0.217105* 0.226885* 0.203607* 0.155989* 0.349235* 0.172802* 0.214067* 0.183967* 0.269231*
Peroxisomes 0.000022 0.000009 0.000013 0.000051 0.000006 0.000049 0.000015 0.000341 0.000014 0.000035 0.000015 0.000165
Vacuole 0.004385 0.003593 0.012267 0.013369 0.002875 0.002539 0.010487 0.017165 0.003603 0.002891 0.010657 0.014867
Vacuole Periphery 0.002699 0.000314 0.000129 0.000847 0.000097 0.000094 0.000263 0.000458 0.001351 0.000167 0.000250 0.000693

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 3.42 -5.33 -1.10 -2.50 3.54 6.64 0.68 -2.60 -2.82 -2.63 7.40 0.02 -2.85 -3.46 -2.85
Bud Neck -0.77 -6.30 -7.94 -7.53 3.41 3.53 -4.77 -13.19 -14.52 -10.70 2.42 -7.09 -14.51 -15.87 -8.25
Bud Site 1.98 -2.46 -4.09 -4.99 -2.24 0.96 -5.43 -7.26 -7.67 -3.95 3.24 -4.34 -7.98 -9.25 -4.08
Cell Periphery 1.51 -7.31 3.92 2.67 8.41 6.86 -2.87 -6.38 -13.12 -3.75 6.46 -3.91 -0.52 -8.36 3.75
Cytoplasm 20.61 29.75 28.37 5.33 -16.87 -4.29 -8.74 27.61 32.26 35.36* 11.24 8.21 34.50 25.66 23.64
Cytoplasmic Foci -1.06 -8.40 0.66 1.68 8.52 3.28 -3.93 -10.45 -11.83 -8.02 2.27 -5.82 -6.49 -8.42 0.31
Eisosomes -0.30 -6.61 -4.67 -4.79 5.66 3.24 -5.42 -10.93 -11.43 -9.64 2.17 -6.99 -11.87 -13.04 -6.20
Endoplasmic Reticulum 5.21 3.50 3.54 -2.18 0.33 5.99 3.19 -5.52 -6.67 -6.36 7.04 5.94 -1.57 -7.15 -6.42
Endosome 2.84 0.15 -2.40 -3.27 -2.48 0.85 -1.62 -2.53 -2.57 -2.32 3.09 0.07 -3.60 -4.10 -3.58
Golgi 5.70 4.10 3.28 -0.78 0.50 3.94 1.37 0.33 -2.63 -0.94 6.91 5.37 2.72 -1.70 -1.12
Lipid Particles 1.05 -5.70 -0.26 -1.94 5.80 4.77 -3.09 -4.63 -5.79 -3.65 4.52 -4.13 -4.45 -7.08 -1.58
Mitochondria 4.56 -2.28 -1.26 -6.58 1.41 3.96 -0.15 -5.84 -6.91 -5.21 6.17 1.12 -5.57 -9.98 -5.55
Mitotic Spindle 2.40 -0.70 -4.50 -5.49 -2.91 -1.28 -0.87 -4.26 -3.63 -3.88 -0.36 -0.61 -6.27 -5.90 -5.59
None -6.43 2.54 -1.62 4.89 -4.29 -1.35 10.60 1.48 2.62 -5.97 -6.09 9.19 0.57 6.02 -7.02
Nuclear Periphery -1.84 -9.71 -11.61 -9.59 6.89 1.30 0.74 -2.75 -10.88 -9.29 1.18 -0.72 -3.80 -15.20 -8.51
Nuclear Periphery Foci 2.82 -0.17 3.33 1.14 4.83 8.23 3.25 -3.39 -7.11 -5.14 7.27 3.85 0.26 -6.70 -3.50
Nucleolus 0.25 -0.45 -3.16 -3.16 -3.13 -0.47 -1.93 -5.64 -5.37 -3.56 0.33 -1.30 -5.49 -5.56 -3.83
Nucleus -21.28 -24.46 -25.33 -0.90 13.80 4.15 11.90 -16.51 -20.40 -26.15 -11.64 -4.85 -24.97 -15.11 -18.83
Peroxisomes 1.37 -0.78 -1.66 -3.24 -1.26 -1.06 -3.37 -1.77 -1.11 -1.66 -0.82 -0.39 -2.15 -1.13 -2.10
Vacuole -2.78 -8.26 -16.13 -14.50 1.89 -1.01 -14.52 -16.38 -16.18 -10.87 -2.45 -16.80 -22.81 -22.00 -10.14
Vacuole Periphery 3.24 3.38 3.07 -0.61 -1.24 0.26 -1.51 -1.37 -1.36 -0.28 3.34 3.21 2.78 -1.62 -1.18
Standard name
Human Ortholog
Description Dimeric hypoxanthine-guanine phosphoribosyltransferase; catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Hpt1

Hpt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hpt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available