Standard name
Human Ortholog
Description Phosphoribosyl-glycinamide transformylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 0.97 0.94 0.92 0.88 0.83 – 0.83 0.74 0.79 0.69 0.99 0.99 1.0 0.98 1.0 0.99 0.98 0.94 0.94 0.94 0.94 0.94
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.09 0.12 0.12 – 0.12 0.17 0.13 0.19 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 2 4 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 2 0 0 1 0 0 0 0 0 0 0 0 0 2 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 3 0 5 – 5 7 10 8 2 1 0 1 1 3 0 0 0 0 0 0
Cytoplasm 178 195 61 88 118 85 182 – 164 173 164 147 179 178 220 83 102 136 167 179 72 86 172 138
Endoplasmic Reticulum 0 0 0 3 0 0 5 – 4 11 7 8 0 0 2 3 3 7 0 0 0 0 3 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 2 – 0 2 2 2 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 1 – 2 6 4 14 0 1 0 1 0 0 0 0 0 0 0 1
Nucleus 0 0 1 0 1 3 4 – 4 8 4 8 0 0 1 1 0 0 0 2 0 1 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 2 5 12 12 27 – 24 39 27 40 0 0 0 1 0 1 0 3 0 0 0 1
Unique Cell Count 178 195 63 94 128 97 220 198 235 207 213 180 179 221 85 102 137 172 190 77 92 183 147
Labelled Cell Count 178 195 64 96 134 101 227 204 251 222 230 182 180 223 90 106 147 172 190 77 92 183 147


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 41.2 35.5 32.7 30.2 27.4 14.4 19.8 – 16.2 15.8 15.7 15.6 47.6 39.3 46.1 44.0 39.9 44.1 34.4 37.1 37.7
Std Deviation (1e-4) 7.7 8.0 6.1 7.6 4.7 4.4 4.0 – 3.0 3.3 3.6 3.4 11.7 9.3 10.9 14.4 12.5 11.3 9.2 8.4 9.8
Intensity Change (Log2) – – – -0.12 -0.25 -1.19 -0.73 – -1.02 -1.05 -1.06 -1.07 0.54 0.26 0.49 0.43 0.29 0.43 0.07 0.18 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.9 -1.2 -2.0 -2.8 – -2.8 -4.0 -3.3 -4.5 1.6 1.6 1.9 0.3 1.8 1.3
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 2.0 2.1 – 2.1 2.7 2.2 3.0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 27.742 32.2835 29.8344 27.7424 28.3979 29.6147 28.3594 40.0389 34.5851 32.0678 37.8603 34.4226 35.4289 39.2568 37.1682 37.6081 33.7875 36.5463
Actin 0.0069 0.0001 0.0002 0.0004 0.0007 0.0023 0.0464 0.0001 0.0142 0.0001 0.0001 0.0006 0.0031 0.001 0.0003 0.0004 0.0127 0.0059
Bud 0.0005 0.0005 0.0001 0.0004 0.0003 0.0001 0.0013 0 0.0008 0.0002 0 0.0001 0.0005 0.0012 0.0034 0 0.0008 0.002
Bud Neck 0.0004 0.0001 0.0002 0.0008 0.0004 0.0007 0.0008 0.0001 0.0004 0.0004 0.0002 0.0003 0.0006 0.0132 0.0002 0.0004 0.0005 0.0024
Bud Periphery 0.0003 0.0001 0 0.0005 0.0001 0 0.0011 0 0.0006 0.0001 0 0.0001 0.0005 0.0004 0.0012 0 0.0007 0.0011
Bud Site 0.0005 0.0001 0.0001 0.0002 0 0.0001 0.0033 0 0.0011 0.0002 0 0 0.0014 0.0164 0.0002 0 0.0023 0.0002
Cell Periphery 0.0001 0 0.0001 0.0001 0 0 0.0003 0 0.0001 0 0 0 0.0001 0.0003 0 0 0.0001 0
Cytoplasm 0.9153 0.9843 0.9642 0.935 0.9857 0.9522 0.832 0.9951 0.9343 0.8585 0.9865 0.896 0.936 0.9536 0.9763 0.9812 0.896 0.9338
Cytoplasmic Foci 0.0075 0.0002 0.0011 0.0024 0.0007 0.0036 0.0154 0.0001 0.0087 0.0241 0.0002 0.0039 0.0068 0.0028 0.0005 0.0006 0.0314 0.0193
Eisosomes 0.0001 0 0 0 0 0 0.0005 0 0.0001 0 0 0 0.0001 0 0 0 0.0006 0
Endoplasmic Reticulum 0.003 0.0003 0.0009 0.0023 0.0004 0.001 0.0089 0.0001 0.0015 0.0006 0.0001 0.0016 0.0051 0.0001 0.001 0.0002 0.0008 0.0006
Endosome 0.0045 0.0001 0.0021 0.0087 0.0001 0.0044 0.0239 0 0.0076 0.0399 0 0.0254 0.0072 0 0.0018 0.0001 0.0013 0.0012
Golgi 0.0014 0 0.0003 0.0004 0 0.0009 0.0045 0 0.0059 0.0086 0 0.004 0.0016 0 0 0 0.0008 0.0004
Lipid Particles 0.0047 0 0.0005 0.0008 0 0.0023 0.0047 0 0.0015 0.0408 0 0.001 0.0015 0.0002 0.0003 0 0.003 0.0007
Mitochondria 0.0067 0.0002 0.0042 0.0057 0.0001 0.0009 0.0075 0 0.0043 0.0025 0 0.0011 0.0016 0.0002 0.0002 0.0001 0.0187 0.0022
None 0.0022 0.0002 0.0009 0.0013 0.0004 0.0005 0.0072 0.0001 0.0008 0.0003 0.0001 0.0004 0.0021 0.0001 0.0004 0.0003 0.0028 0.0004
Nuclear Periphery 0.008 0.0018 0.0028 0.0052 0.0021 0.0046 0.013 0.0005 0.0012 0.0016 0.0011 0.0407 0.0057 0.0007 0.0018 0.0016 0.0028 0.0026
Nucleolus 0.0022 0 0.0012 0.0005 0 0.0003 0.0003 0 0.0002 0.0003 0 0.0002 0.0004 0.0003 0 0 0.0011 0.0002
Nucleus 0.0264 0.0112 0.0151 0.0214 0.0085 0.0224 0.0139 0.0038 0.0091 0.0102 0.0113 0.0186 0.02 0.0087 0.0105 0.0146 0.0149 0.0243
Peroxisomes 0.0009 0 0.0001 0.0002 0 0.0011 0.0039 0 0.0012 0.0042 0 0.0001 0.0006 0.0003 0 0 0.0015 0.0012
Punctate Nuclear 0.0027 0.0001 0.0003 0.0004 0.0002 0.001 0.0079 0.0001 0.0005 0.0019 0.0001 0.0004 0.0024 0.0001 0.0002 0.0003 0.006 0.0004
Vacuole 0.004 0.0004 0.0041 0.0076 0.0003 0.0012 0.002 0 0.0045 0.0033 0.0001 0.0022 0.0021 0.0001 0.0014 0.0001 0.0006 0.0007
Vacuole Periphery 0.0015 0.0001 0.0015 0.0056 0.0001 0.0004 0.0012 0 0.0013 0.0024 0 0.0035 0.0005 0 0.0003 0 0.0005 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 59.0193 58.1121 54.4695 61.8609 53.2321 51.2927 74.2195 72.0089 71.6352 65.029
Translational Efficiency 1.6156 1.3015 1.4253 1.0601 1.2924 2.0305 1.2379 1.3146 1.4087 1.1767

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1926 1475 110 1703 2667 2244 1192 1185 4593 3719 1302 2888

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2054.96 2579.63 2732.80 2060.90 2678.20 2777.18 1953.59 2110.72 2416.85 2698.83 2019.42 2081.34
Standard Deviation 437.80 724.09 338.43 379.15 491.56 522.39 347.10 388.08 561.48 618.02 408.58 383.62
Intensity Change Log 2 — 0.328054 0.411270 0.004164 — 0.052357 -0.455136 -0.343528 — 0.178570 -0.014327 -0.182196

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000189 0.000137 0.000687 0.000303 0.000020 0.000070 0.000194 0.000133 0.000091 0.000097 0.000236 0.000233
Bud Neck 0.000445 0.000428 0.005809 0.001414 0.000159 0.000341 0.001810 0.001288 0.000279 0.000375 0.002148 0.001362
Bud Site 0.002281 0.001693 0.003265 0.006429 0.000331 0.001033 0.003377 0.003633 0.001149 0.001295 0.003368 0.005282
Cell Periphery 0.000202 0.000203 0.001491 0.000195 0.000037 0.000089 0.000112 0.000129 0.000106 0.000134 0.000228 0.000168
Cytoplasm 0.835365* 0.758734* 0.465544* 0.736515* 0.789952* 0.765454* 0.704319* 0.751194* 0.808995* 0.762789* 0.684146* 0.742538*
Cytoplasmic Foci 0.007175 0.003374 0.025696 0.006236 0.001615 0.003125 0.005528 0.006560 0.003946 0.003224 0.007232 0.006369
Eisosomes 0.000001 0.000001 0.000007 0.000002 0.000000 0.000001 0.000004 0.000002 0.000000 0.000001 0.000004 0.000002
Endoplasmic Reticulum 0.000137 0.000074 0.000298 0.000159 0.000023 0.000045 0.000350 0.000059 0.000070 0.000056 0.000346 0.000118
Endosome 0.000140 0.000150 0.001075 0.001419 0.000027 0.000277 0.002037 0.000476 0.000074 0.000227 0.001955 0.001032
Golgi 0.001969 0.000824 0.001761 0.002620 0.000156 0.001368 0.001085 0.000764 0.000916 0.001152 0.001143 0.001858
Lipid Particles 0.000242 0.000230 0.005952 0.000773 0.000069 0.000152 0.000465 0.000402 0.000141 0.000183 0.000929 0.000621
Mitochondria 0.000179 0.000106 0.000358 0.000168 0.000090 0.000099 0.000226 0.000170 0.000127 0.000101 0.000237 0.000169
Mitotic Spindle 0.002040 0.001130 0.007015 0.029709 0.000260 0.002611 0.004873 0.016117 0.001007 0.002024 0.005054 0.024132
None 0.009477 0.016730 0.006479 0.010086 0.019406 0.019697 0.007735 0.011619 0.015242 0.018520 0.007629 0.010715
Nuclear Periphery 0.000040 0.000068 0.001023 0.000259 0.000036 0.000072 0.000276 0.000300 0.000038 0.000071 0.000339 0.000276
Nuclear Periphery Foci 0.000978 0.001555 0.005336 0.004152 0.000448 0.001022 0.001702 0.001516 0.000670 0.001233 0.002009 0.003070
Nucleolus 0.000081 0.000127 0.000168 0.000133 0.000053 0.000095 0.000684 0.000149 0.000065 0.000107 0.000640 0.000140
Nucleus 0.135766* 0.209927* 0.439310* 0.180080* 0.186005* 0.201499* 0.224541* 0.187836* 0.164938* 0.204842* 0.242686* 0.183262*
Peroxisomes 0.000019 0.000014 0.000049 0.000118 0.000006 0.000010 0.000134 0.000047 0.000011 0.000012 0.000127 0.000089
Vacuole 0.002596 0.004328 0.028442 0.016008 0.001212 0.002716 0.039438 0.015973 0.001792 0.003355 0.038509 0.015993
Vacuole Periphery 0.000679 0.000166 0.000234 0.003221 0.000097 0.000225 0.001112 0.001633 0.000341 0.000202 0.001038 0.002570

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.54 -5.67 -1.29 -3.78 5.53 -7.11 -6.80 -14.13 -8.02 1.50 -0.58 -5.19 -4.89 -7.32 1.87
Bud Neck -4.31 -6.52 -10.72 -6.01 5.64 -4.98 -10.37 -12.94 -10.15 1.13 -6.42 -11.52 -17.31 -12.09 4.63
Bud Site 0.84 -4.43 -4.00 -3.95 1.84 -4.97 -8.12 -14.00 -9.71 -0.64 -1.07 -6.98 -9.67 -7.88 -0.63
Cell Periphery -1.79 -7.41 -0.84 0.92 7.30 -5.72 -10.22 -11.89 -5.53 -2.38 -4.03 -7.30 -6.68 -2.80 3.34
Cytoplasm 15.59 22.05 21.48 5.42 -15.36 7.47 22.48 15.68 10.16 -4.55 15.95 29.70 25.15 11.22 -10.44
Cytoplasmic Foci 1.87 -7.71 -2.46 -4.56 7.30 -6.63 -10.24 -13.07 -8.34 -3.55 -2.21 -8.71 -11.88 -9.64 0.80
Eisosomes -3.06 -7.47 -12.70 -9.37 5.57 -9.78 -14.34 -16.69 -14.39 1.67 -6.46 -15.22 -20.36 -16.69 3.91
Endoplasmic Reticulum 2.28 -3.71 2.28 -0.49 4.37 -3.29 -9.75 -10.43 -4.19 7.23 1.03 -8.52 -1.64 -2.38 7.74
Endosome -0.66 -3.09 -3.06 -2.29 1.87 -2.64 -3.80 -6.41 -0.55 2.50 -2.60 -3.97 -5.05 -1.98 2.31
Golgi 1.90 0.22 2.14 0.13 2.39 -2.44 -2.19 -1.37 2.05 1.09 -0.30 1.16 1.36 1.43 0.31
Lipid Particles -3.59 -3.41 -7.77 -6.27 2.68 -4.21 -9.64 -12.56 -10.44 -2.80 -5.31 -6.61 -11.70 -9.88 -0.40
Mitochondria 3.35 -6.01 3.12 -0.41 8.25 -1.51 -4.80 -3.50 -2.37 2.32 2.33 -4.06 0.47 -2.09 4.41
Mitotic Spindle 1.26 -1.84 -4.25 -4.69 -1.51 -3.76 -4.11 -5.16 -3.43 -3.27 -2.43 -3.47 -6.66 -5.49 -4.35
None -4.93 4.75 3.28 7.82 -2.32 1.21 14.68 12.50 10.43 -2.37 -1.89 14.06 12.78 13.40 -2.03
Nuclear Periphery -8.99 -9.08 -13.40 -8.86 7.65 -4.00 -19.36 -13.75 -10.76 -0.17 -6.72 -19.70 -18.97 -13.43 6.38
Nuclear Periphery Foci -3.40 -4.63 -2.98 -0.81 3.19 -2.24 -8.74 -8.06 -2.78 0.32 -3.66 -9.93 -5.25 -2.20 0.67
Nucleolus -1.45 -7.59 -3.32 0.72 5.11 -3.03 -2.47 -10.36 -3.52 1.66 -2.41 -2.51 -8.56 -0.36 1.88
Nucleus -14.99 -15.20 -8.68 7.15 12.95 -5.08 -10.00 0.62 4.73 9.14 -13.70 -17.63 -4.17 8.56 14.09
Peroxisomes 0.17 -6.15 -3.17 -3.12 1.69 -4.29 -3.89 -6.09 -4.96 1.45 -1.58 -4.04 -5.07 -4.61 0.93
Vacuole -5.46 -7.52 -22.88 -20.10 0.60 -8.31 -20.27 -20.37 -19.29 -1.80 -9.05 -21.32 -30.75 -28.39 -0.18
Vacuole Periphery 2.77 2.16 -0.16 -1.33 -1.06 -1.55 -1.62 -0.90 -0.49 0.22 1.77 -0.29 -0.82 -1.38 -0.56
Standard name
Human Ortholog
Description Phosphoribosyl-glycinamide transformylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Ade8

Ade8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ade8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available