Standard name
Human Ortholog
Description SUMO E3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to primarily cytoplasmic proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; required for sumoylation of septins and histone H3 variant Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; localizes to the septin ring; acts as an adapter between E2, Ubc9p and substrates; tends to compensate for survival of DNA damage in absence of Nfi1p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.06 0.06 0.07 0.08 0.1 0.1 0.08 0.06 0.06 0.08 0.07 0.13 0.13 0.12 0.1 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0.23 0 0.08 0.05 0.27 0.47 0.31 0.62 0.7 0.79 0.49 0 0 0 0 0 0.08 0 0 0 0 0 0
Nucleus 0.86 0.84 0.85 0.85 0.83 0.72 0.68 0.7 0.7 0.67 0.6 0.69 0.92 0.85 0.85 0.74 0.73 0.77 0.78 0.78 0.72 0.66 0.58 0.58
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.1 0.07
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0.06 0 0 0.06 0 0 0 0.05 0 0 0 0.05 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.08 0.07 0.05 0.1 0.11 0.17 0.14 0.18
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 1 3 1 0 1 0 0 1 0 0 0 0 0 0 0 1 1 1 1 1 2 0
Bud 1 0 1 0 0 1 3 4 5 5 6 4 1 1 0 1 1 1 3 2 3 1 6 7
Bud Neck 0 0 5 0 6 0 9 11 1 3 2 2 0 0 0 0 0 0 9 4 2 1 5 2
Bud Site 0 1 0 1 6 4 8 4 10 18 10 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 13 10 22 11 17 20 31 35 20 13 15 23 19 46 48 62 45 61 1 3 3 0 5 5
Endoplasmic Reticulum 3 2 0 0 1 0 0 2 0 0 0 1 0 1 1 23 15 16 1 0 3 1 6 0
Endosome 5 0 5 8 2 7 3 2 1 1 0 1 0 3 5 16 23 8 10 5 10 2 6 8
Golgi 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 1 2 1 6 0 0 1 0 0
Mitochondria 48 72 15 15 14 66 147 102 155 166 198 139 2 2 2 22 15 38 7 6 7 4 12 11
Nucleus 293 261 293 165 213 176 215 234 177 158 151 198 240 289 312 397 342 356 281 266 273 148 225 186
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 2 2 0 0 0 3 0 1 0 0 2 0 2 2
Nucleolus 2 0 0 0 0 3 0 0 2 3 1 2 4 2 2 3 6 1 6 8 9 13 36 22
Peroxisomes 4 1 1 3 2 4 0 1 0 0 0 2 0 0 1 3 6 1 3 0 1 0 0 0
SpindlePole 2 3 15 5 16 8 14 19 4 7 5 15 6 8 8 26 24 13 2 5 10 4 10 5
Vac/Vac Membrane 7 16 9 2 4 2 5 5 1 1 2 4 0 4 7 42 38 31 18 34 42 38 55 57
Unique Cell Count 342 309 345 194 257 245 315 334 252 236 250 285 261 342 366 534 467 462 360 342 380 226 387 320
Labelled Cell Count 379 368 368 213 282 292 436 419 376 376 392 404 273 356 386 600 517 529 360 342 380 226 387 320


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 4.5 5.6 5.0 5.1 4.4 4.2 4.5 3.8 3.6 3.3 4.1 6.2 6.3 6.4 6.9 7.2 6.3 6.0 6.3 6.8
Std Deviation (1e-4) 1.1 0.6 1.2 1.2 1.2 1.2 1.1 1.3 0.9 1.1 0.9 1.2 1.6 1.7 1.9 1.6 1.4 1.2 1.8 2.1 2.2
Intensity Change (Log2) – – – -0.16 -0.13 -0.37 -0.43 -0.32 -0.58 -0.62 -0.76 -0.47 0.14 0.17 0.19 0.29 0.36 0.17 0.09 0.16 0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.3 3.3 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.5364 2.5655 2.6139 2.2604 2.2995 2.6932 1.3759 0.7799 0.7624 1.0712 0.8613 0.6656 0.6463 0.7915 0.4317 0.4478 0.5498 0.6454
Actin 0.0469 0.005 0.0131 0.059 0.0032 0.0009 0.037 0 0.0101 0.0333 0.0344 0.0001 0.0063 0 0 0.0001 0.0005 0.0001
Bud 0.0005 0.0003 0.0003 0.0008 0.0002 0 0.0011 0 0.0003 0.001 0.0004 0 0.0001 0 0.0003 0 0.0002 0.0011
Bud Neck 0.0019 0.0006 0.003 0.0055 0.2575 0.1416 0.0154 0.0006 0.0003 0.0914 0.138 0.0833 0.001 0.0007 0.0001 0.0726 0.1324 0.0495
Bud Periphery 0.0006 0.0002 0.0006 0.0009 0.0004 0.0001 0.0026 0 0.0005 0.0026 0.0009 0.0001 0.0001 0 0 0 0.0002 0.0005
Bud Site 0.0043 0.0015 0.0102 0.0098 0.0013 0.0002 0.0051 0.0061 0.0032 0.0074 0.001 0.0001 0.0001 0.0001 0.0001 0 0.0012 0.0004
Cell Periphery 0.0002 0.0001 0.0002 0.0002 0.0002 0.0001 0.0007 0 0.0001 0.0013 0.0001 0 0 0 0 0 0.0001 0
Cytoplasm 0.0062 0.0028 0.0023 0.0009 0.0135 0.0012 0.0045 0.0048 0.0015 0.0061 0.0075 0.0004 0.0013 0.0008 0.0017 0.0004 0.004 0.0005
Cytoplasmic Foci 0.0027 0.0018 0.0024 0.0212 0.0121 0.0013 0.0245 0.0034 0.0018 0.0308 0.0093 0.0001 0.0007 0 0.0013 0 0.0032 0.0001
Eisosomes 0.0005 0 0.0002 0.0004 0.0002 0.0002 0.0013 0 0.0001 0.0006 0.0002 0 0.0002 0 0 0 0 0
Endoplasmic Reticulum 0.0013 0.0004 0.0014 0.0003 0.0016 0.0006 0.0024 0.0001 0.0008 0.0045 0.0049 0 0.0015 0.0002 0 0 0.0003 0
Endosome 0.0022 0.0021 0.002 0.0069 0.0228 0.0047 0.0199 0.0015 0.009 0.0353 0.0351 0.0012 0.0018 0.0002 0 0 0.0005 0
Golgi 0.0031 0.0019 0.0016 0.0181 0.0036 0.0011 0.015 0.0001 0.0036 0.011 0.0093 0.0005 0.0006 0 0 0 0.0003 0
Lipid Particles 0.0016 0.0006 0.0013 0.0094 0.008 0.0072 0.019 0.0001 0.0064 0.0144 0.0173 0 0.0014 0.0001 0 0 0.0006 0
Mitochondria 0.007 0.0009 0.0162 0.0093 0.005 0.0008 0.0247 0.0001 0.0043 0.0527 0.0044 0.0046 0.0007 0.0001 0.0002 0.0001 0.0005 0
None 0.0703 0.0036 0.0198 0.001 0.0343 0.0107 0.0082 0.0006 0.0107 0.0009 0.0039 0.0001 0.0031 0.0029 0.0025 0 0.0007 0.0002
Nuclear Periphery 0.0087 0.004 0.0058 0.0062 0.0041 0.0015 0.0174 0.0033 0.0086 0.005 0.0274 0.0009 0.0066 0.0065 0.0013 0.0024 0.0067 0.0004
Nucleolus 0.0127 0.0134 0.0244 0.0114 0.0311 0.0303 0.0118 0.0097 0.0082 0.0129 0.0245 0.0188 0.0102 0.0124 0.0066 0.0097 0.073 0.0203
Nucleus 0.811 0.9359 0.8685 0.7894 0.4724 0.7828 0.7585 0.9621 0.9167 0.6541 0.5953 0.8748 0.9435 0.9428 0.9305 0.892 0.4942 0.8782
Peroxisomes 0.0041 0.0003 0.0011 0.0443 0.0044 0.0026 0.0118 0.0001 0.0012 0.0066 0.0104 0 0.0009 0 0 0 0.0002 0
Punctate Nuclear 0.0131 0.0242 0.0239 0.0041 0.1229 0.0108 0.0133 0.0068 0.0096 0.0086 0.073 0.0144 0.0193 0.0329 0.0552 0.0223 0.2809 0.0485
Vacuole 0.0007 0.0002 0.0012 0.0006 0.001 0.0008 0.0037 0.0003 0.0016 0.0152 0.0013 0.0004 0.0003 0.0001 0 0.0001 0.0003 0.0001
Vacuole Periphery 0.0004 0.0001 0.0006 0.0004 0.0004 0.0002 0.0021 0.0001 0.0011 0.0042 0.0016 0.0002 0.0003 0.0002 0 0 0.0001 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 8.1282 8.7193 7.0093 10.7529 9.2213 5.2804 10.561 10.0411 10.418 12.0998
Translational Efficiency 0.6126 0.5629 0.7983 0.3959 0.4526 1.0813 0.4876 0.6606 0.6958 0.5171

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1751 864 62 1252 1379 1244 580 191 3130 2108 642 1443

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 664.07 781.31 899.01 846.22 647.98 753.01 865.76 848.35 656.98 764.61 868.97 846.50
Standard Deviation 73.21 106.45 127.48 119.79 103.02 144.89 114.04 115.82 87.97 131.25 115.82 119.27
Intensity Change Log 2 — 0.234560 0.437002 0.349697 — 0.216720 0.418018 0.388710 — 0.225777 0.427657 0.369097

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000055 0.000509 0.001260 0.002005 0.000155 0.000676 0.000527 0.003546 0.000099 0.000607 0.000597 0.002209
Bud Neck 0.044632 0.028091 0.020841 0.170391 0.032789 0.036949 0.047625 0.089316 0.039414 0.033318 0.045038 0.159659
Bud Site 0.004434 0.004333 0.004404 0.149560* 0.004551 0.007386 0.012986 0.159868* 0.004486 0.006135 0.012157 0.150924*
Cell Periphery 0.000204 0.000119 0.000091 0.000352 0.000227 0.000310 0.000101 0.000348 0.000214 0.000232 0.000100 0.000352
Cytoplasm 0.040978 0.011607 0.008043 0.046085 0.037372 0.022128 0.011558 0.097252 0.039389 0.017816 0.011219 0.052858
Cytoplasmic Foci 0.009109 0.003840 0.004079 0.010072 0.009730 0.007694 0.000937 0.003741 0.009383 0.006115 0.001240 0.009234
Eisosomes 0.000033 0.000037 0.000064 0.000086 0.000034 0.000043 0.000043 0.000064 0.000034 0.000041 0.000045 0.000084
Endoplasmic Reticulum 0.000875 0.004120 0.009146 0.008806 0.001633 0.004146 0.004807 0.005293 0.001209 0.004135 0.005226 0.008341
Endosome 0.001045 0.001592 0.002980 0.012540 0.001927 0.004125 0.000778 0.002650 0.001433 0.003087 0.000990 0.011231
Golgi 0.000493 0.000895 0.000755 0.004904 0.000620 0.002492 0.000086 0.001339 0.000549 0.001837 0.000151 0.004432
Lipid Particles 0.001276 0.001428 0.001278 0.001278 0.001633 0.002507 0.000657 0.000723 0.001433 0.002065 0.000717 0.001204
Mitochondria 0.001132 0.002642 0.006090 0.008587 0.002142 0.008675 0.000728 0.001889 0.001577 0.006203 0.001246 0.007701
Mitotic Spindle 0.001395 0.005345 0.008653 0.065130 0.001141 0.004759 0.003247 0.023223 0.001283 0.004999 0.003769 0.059583
None 0.009823 0.009250 0.003021 0.007182 0.031879 0.011862 0.003335 0.020114 0.019541 0.010792 0.003305 0.008894
Nuclear Periphery 0.000995 0.000687 0.000649 0.002179 0.000854 0.002368 0.000642 0.001197 0.000933 0.001679 0.000643 0.002049
Nuclear Periphery Foci 0.000225 0.000519 0.000725 0.002350 0.000942 0.000951 0.000289 0.000462 0.000541 0.000774 0.000331 0.002100
Nucleolus 0.007874 0.013644 0.007366 0.004338 0.015371 0.018082 0.006065 0.002131 0.011177 0.016263 0.006190 0.004046
Nucleus 0.871348* 0.905467* 0.913040* 0.453604* 0.850921* 0.852379* 0.899813* 0.572234* 0.862348* 0.874138* 0.901091* 0.469307*
Peroxisomes 0.001503 0.003122 0.001563 0.004007 0.003003 0.003687 0.000400 0.003136 0.002164 0.003455 0.000512 0.003891
Vacuole 0.002343 0.002133 0.004545 0.038006 0.002753 0.006963 0.005240 0.011279 0.002523 0.004983 0.005173 0.034468
Vacuole Periphery 0.000227 0.000620 0.001406 0.008537 0.000324 0.001818 0.000136 0.000196 0.000270 0.001327 0.000259 0.007433

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.69 -3.40 -11.91 -7.77 -0.67 -1.74 -11.55 -3.37 -2.53 -2.46 -2.99 -12.12 -11.30 -5.66 -5.95
Bud Neck 2.87 1.81 -8.01 -10.54 -5.45 -0.75 -0.92 -1.73 -1.33 -1.06 1.53 0.39 -9.43 -10.56 -7.76
Bud Site 0.11 0.08 -14.56 -14.49 -13.27 -2.20 -3.13 -4.40 -4.18 -3.72 -1.89 -3.21 -15.16 -14.73 -12.25
Cell Periphery 2.17 2.55 -1.59 -5.91 -6.11 -1.23 3.29 -0.60 0.80 -4.23 -0.49 4.24 -1.64 -0.64 -9.40
Cytoplasm 9.45 6.05 -1.50 -9.53 -6.73 4.08 7.56 -1.70 -3.12 -4.25 8.76 10.90 -2.41 -8.69 -10.44
Cytoplasmic Foci 3.40 2.33 3.40 -0.30 -0.48 1.81 6.94 4.08 2.77 -1.83 3.24 9.53 4.85 1.72 -4.51
Eisosomes -1.41 -3.14 -13.01 -10.47 -0.94 -1.91 -4.18 -6.11 -5.43 -4.25 -2.54 -5.59 -14.80 -12.53 -6.89
Endoplasmic Reticulum -10.17 -2.81 -16.82 -10.43 -0.39 -6.37 -12.34 -9.79 -7.45 -4.59 -11.22 -12.34 -18.49 -12.39 -5.33
Endosome -0.86 -0.90 -5.32 -4.06 -1.34 -2.33 3.32 -0.75 1.67 -3.31 -2.79 1.81 -4.91 -2.32 -5.57
Golgi -0.57 -0.60 -2.32 -1.14 -1.00 -2.24 3.33 0.20 2.18 -1.35 -2.28 2.67 -2.30 0.48 -3.57
Lipid Particles -0.94 -0.49 -0.59 0.55 0.21 -1.50 3.19 0.80 2.13 -2.07 -2.08 3.08 0.41 2.47 -2.93
Mitochondria -2.30 -1.04 -3.98 -2.03 0.07 -5.31 3.06 -0.07 5.30 -3.27 -6.05 0.60 -3.58 1.46 -3.66
Mitotic Spindle -2.15 -1.06 -10.28 -8.71 -5.37 -3.51 -2.09 -3.64 -3.13 -3.14 -3.74 -2.24 -10.93 -9.74 -9.46
None 0.20 4.64 3.12 1.98 -1.67 5.81 9.18 5.81 0.59 -3.25 4.61 10.44 7.72 3.19 -2.63
Nuclear Periphery 0.92 0.73 -7.92 -12.93 -10.67 -2.77 -0.72 -5.37 -1.24 -5.73 -2.18 -0.35 -10.15 -5.94 -12.22
Nuclear Periphery Foci -2.61 -1.23 -5.13 -3.80 -1.08 0.80 1.96 -0.90 -1.46 -2.17 -0.56 0.35 -3.77 -3.38 -3.87
Nucleolus -3.69 -0.35 5.01 7.63 1.16 -0.96 5.24 7.05 7.82 1.20 -3.74 3.20 9.70 12.06 2.49
Nucleus -3.45 -1.47 21.43 22.97 10.58 -0.48 -4.58 5.88 6.08 7.81 -1.79 -4.26 22.06 22.68 21.75
Peroxisomes -2.24 -0.31 -0.97 1.72 -0.05 -0.36 4.65 2.40 2.51 -2.26 -1.98 5.16 0.77 2.55 -4.38
Vacuole 0.08 -2.51 -16.63 -16.65 -15.03 -3.39 -4.93 -5.76 -5.31 -4.97 -3.29 -5.48 -17.58 -16.74 -14.96
Vacuole Periphery -1.33 -1.07 -3.71 -3.01 -1.44 -3.33 3.29 1.20 3.51 -3.84 -3.63 0.20 -3.65 -1.87 -3.65
Protein Complexes26S Proteasome complex37S mitochondrial small ribosomal subunit54S mitochondrial large ribosomal subunit60S cytosolic large ribosomal subunitAar2-U5 snRNPAnaphase-Promoting Complex variant 1Anaphase-Promoting Complex variant 2Anaphase-Promoting Complex variant 3Anaphase-Promoting Complex variant 4Arp2/3 complexATG1 kinase complexBLOC-1 complexCcr4-Not complexCentral kinetochore CTF19 complexCleavage and polyadenylation specificity factorcomplexCMG helicase complexCMG-Pol epsilon complexCOMPASS complexCOP9 signalosome COPI vesicle coat complexCoQ6 biosynthesis complexCore mediator complexCORVET complexCTF18-RFC complexCURI complex variant 1CURI complex variant 2CURI complex variant 3Cytoplasmic exosome complex, DIS3 variantDASH complexDNA-directed RNA Polymerase I complexDNA-directed RNA polymerase II complexDNA-directed RNA polymerase III complexDNA replication factor C complexEGO complexELG1-RFC complex Elongator holoenzyme complexEndoplasmic Reticulum Membrane ComplexEukaryotic translation initiation factor 2BcomplexEukaryotic translation initiation factor 3core complexExocystExomer complexFAR complexGeneral transcription factor complex TFIIDGeneral transcription factor complex TFIIHGET4-GET5 transmembrane domain recognition complexGID ubiquitin ligase complexGin4 complexGolgi transport complexGSE complexHMC complexHOPS complexINO80 chromatin remodeling complexLSM1-7 complexLsm1-7-Pat1 complexLSM2-7 complexLSM2-8 complexMCM complexMICOS complexMitochondrial electron transport complex IIIMitochondrial inner membrane pre-sequence translocasecomplexMitochondrial outer membrane translocase holocomplexMitochondrial proton-transporting ATP synthase complexMitochondrial pyruvate dehydrogenase complexNineTeen complexNOP8 60s ribosome pre-assembly complexNuA3 histone acetyltransferase complexNuA4 histone acetyltransferase complexNuclear condensin complexNuclear/nucleolar exosome complex, DIS3-RRP6 variantNuclear pore complexNucleolar ribonuclease MRP complexNucleolar ribonuclease P complexOligosaccharyl transferase complex variant 1Oligosaccharyl transferase complex variant 2PAF1 complexPAS complexPhosphatidylinositol 3-kinase complex IPolarisomePrefoldin complexPRP19-associated complexRad17 RFC-like complexRetromer complexRPD3L histone deacetylase complexRPD3S histone deacetylase complexRQC complexRSC complex variant RSC1RSC complex variant RSC2SAGA complexSCF-Met30 ubiquitin ligase complexSCF-Ufo1 ubiquitin ligase complexSEA complexSeptin complexSET3C histone deacetylase complexSignal recognition particleSLIK (SAGA-like) complexSmall ribosomal subunit processome, variant1Small ribosomal subunit processome, variant2Small ribosomal subunit processome, variant3SMC5-SMC6 SUMO ligase complexSm complexSpliceosomal commitment complexSPOTS complexSWI/SNF chromatin remodelling complexSwr1 chromatin remodelling complexTHO complexTranscription factor TFIIIC complexTranslocon complexTRAPPIII protein complexTRAPPII protein complexTRAPPI protein complexTREX complexU1 snRNPU2 snRNPU4 snRNPU4/U6 snRNPU4/U6.U5 tri-snRNP complexU5 snRNPU6 snRNP complexUTP-A complexUTP-B complexUTP-C complex variant 1UTP-C complex variant 2UTP-C complex variant 3Vacuolar proton translocating ATPase complex,Golgi variantVacuolar proton translocating ATPase complex,vacuole variantCellular Components••••••••••••••••actinbud neckcell peripherycytoplasmendosomeERGolgimitochondrionmixednuclear peripherynucleolusnucleusperoxisomespindle polevacuolar membranevacuole
Standard name
Human Ortholog
Description SUMO E3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to primarily cytoplasmic proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; required for sumoylation of septins and histone H3 variant Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; localizes to the septin ring; acts as an adapter between E2, Ubc9p and substrates; tends to compensate for survival of DNA damage in absence of Nfi1p
Localization
Cell Percentages nucleus (89%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Siz1

Siz1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Siz1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available