Standard name | |
---|---|
Human Ortholog | |
Description | Endoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 7 | 0 | 5 | 13 | 4 | 9 | 1 | 9 | 6 | 6 | 13 | 10 | 5 | 16 | 5 | 12 | 1 | 0 | 5 | 10 | 28 | 22 |
Bud | 0 | 0 | 0 | 2 | 2 | 5 | 2 | 6 | 7 | 4 | 7 | 6 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 3 | 7 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 2 | 9 | 9 | 5 | 3 | 3 | 5 | 4 | 0 | 6 | 22 | 27 | 29 | 18 | 29 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 13 | 22 | 22 | 56 | 55 | 59 | 53 | 48 | 71 | 62 | 60 | 40 | 94 | 91 | 21 | 34 | 31 | 1 | 3 | 5 | 7 | 11 | 9 |
Endoplasmic Reticulum | 44 | 64 | 62 | 19 | 35 | 135 | 149 | 52 | 76 | 53 | 78 | 50 | 118 | 82 | 77 | 1 | 1 | 4 | 90 | 136 | 119 | 282 | 520 | 373 |
Endosome | 2 | 2 | 13 | 17 | 34 | 12 | 8 | 18 | 14 | 18 | 6 | 22 | 7 | 16 | 21 | 1 | 0 | 0 | 2 | 6 | 9 | 8 | 23 | 30 |
Golgi | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 17 | 20 | 9 | 0 | 5 | 3 | 3 | 7 | 7 |
Mitochondria | 22 | 4 | 2 | 28 | 21 | 75 | 82 | 62 | 86 | 63 | 79 | 42 | 0 | 13 | 1 | 4 | 5 | 7 | 5 | 7 | 18 | 17 | 23 | 18 |
Nucleus | 5 | 2 | 16 | 20 | 4 | 14 | 17 | 43 | 29 | 28 | 21 | 35 | 8 | 22 | 13 | 4 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 0 |
Nuclear Periphery | 84 | 91 | 100 | 32 | 5 | 45 | 68 | 45 | 54 | 54 | 36 | 31 | 83 | 85 | 72 | 7 | 11 | 8 | 123 | 145 | 217 | 62 | 75 | 60 |
Nucleolus | 0 | 3 | 5 | 1 | 0 | 2 | 3 | 4 | 0 | 1 | 4 | 2 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 |
SpindlePole | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 1 | 3 | 2 |
Vac/Vac Membrane | 108 | 157 | 138 | 135 | 320 | 146 | 203 | 237 | 190 | 216 | 126 | 204 | 87 | 270 | 260 | 20 | 44 | 51 | 15 | 24 | 18 | 16 | 32 | 36 |
Unique Cell Count | 207 | 272 | 295 | 240 | 431 | 418 | 500 | 458 | 420 | 442 | 359 | 390 | 310 | 541 | 494 | 107 | 121 | 129 | 250 | 337 | 410 | 423 | 746 | 578 |
Labelled Cell Count | 274 | 339 | 366 | 280 | 492 | 511 | 601 | 534 | 508 | 522 | 429 | 458 | 363 | 620 | 572 | 120 | 140 | 151 | 250 | 337 | 410 | 423 | 746 | 578 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.1 | 10.8 | 8.3 | 7.8 | 8.5 | 7.5 | 7.2 | 7.7 | 7.3 | 7.4 | 6.7 | 7.3 | 8.9 | 8.9 | 9.6 | 14.1 | 14.1 | 14.0 | 9.0 | 9.4 | 10.0 |
Std Deviation (1e-4) | 1.1 | 1.7 | 1.2 | 1.4 | 1.4 | 1.3 | 1.3 | 1.3 | 1.5 | 1.6 | 1.4 | 1.5 | 1.4 | 1.5 | 1.7 | 2.7 | 2.7 | 2.7 | 1.8 | 1.6 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.04 | -0.15 | -0.21 | -0.1 | -0.19 | -0.17 | -0.31 | -0.19 | 0.1 | 0.11 | 0.22 | 0.77 | 0.77 | 0.76 | 0.13 | 0.18 | 0.27 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0.6 | 0 | -0.4 | 0 | -0.3 | -0.6 | -0.8 | 1.3 | -0.5 | 0 | 4.8 | 0 | 3.2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 4.1 | 9.3 | 6.8 | 8.4 |
Cytoplasm | 0.7 | 2.4 | 2.4 | 2.0 | 1.8 | 1.8 | 3.4 | 3.7 | 3.2 | 2.2 | 4.0 | 4.3 | 3.5 | 5.6 | 4.7 |
Endoplasmic Reticulum | -4.2 | -5.0 | 3.3 | 2.7 | -3.6 | -1.0 | -3.3 | 0.2 | -2.9 | 4.6 | -2.1 | -1.9 | -4.9 | -5.2 | -4.7 |
Endosome | 1.3 | 1.9 | -1.1 | -2.4 | -0.3 | -0.7 | -0.2 | -2.1 | 0.7 | -1.5 | -1.1 | -0.1 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 6.8 | 0 |
Mitochondria | 5.5 | 3.2 | 7.3 | 7.0 | 6.2 | 7.9 | 6.4 | 8.2 | 5.3 | 0 | 1.8 | 0 | 0 | 0 | 0 |
Nucleus | 1.3 | -3.6 | -1.4 | -1.4 | 2.0 | 0.8 | 0.5 | 0.2 | 1.8 | -1.8 | -0.9 | -2.0 | -0.7 | 0 | 0 |
Nuclear Periphery | -5.5 | -12.3 | -7.6 | -6.8 | -8.2 | -6.7 | -7.1 | -7.5 | -8.6 | -1.9 | -6.1 | -6.4 | -5.5 | -5.2 | -6.0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.2 | 7.5 | -3.2 | -1.7 | 1.3 | -0.4 | 0.6 | -3.0 | 1.4 | -4.8 | 0.9 | 1.6 | -5.1 | -1.9 | -1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.976 | 5.4095 | 5.4274 | 5.2548 | 4.3895 | 5.2397 | 5.6348 | 5.9462 | 5.4248 | 5.3242 | 4.784 | 5.3946 | 7.3751 | 6.8241 | 6.3947 | 6.5157 | 5.7732 | 6.6898 |
Actin | 0.0124 | 0.0002 | 0.0002 | 0.0003 | 0.0029 | 0.0003 | 0.0341 | 0.0003 | 0.0006 | 0.0004 | 0.0031 | 0.0008 | 0.0018 | 0.0007 | 0.0088 | 0.0017 | 0.0015 | 0.0007 |
Bud | 0.0001 | 0.0001 | 0 | 0 | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0014 | 0.0003 | 0.0014 | 0 | 0 |
Bud Neck | 0.0019 | 0.0003 | 0.0001 | 0 | 0.0015 | 0.0002 | 0.0017 | 0.0002 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0009 | 0.0002 | 0.0001 | 0.0002 |
Bud Periphery | 0.0001 | 0.0001 | 0 | 0 | 0.0005 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0005 | 0.0006 | 0.0005 | 0 | 0 |
Bud Site | 0.0011 | 0.0004 | 0 | 0 | 0.0006 | 0 | 0.0007 | 0.0001 | 0 | 0 | 0.0014 | 0 | 0.0003 | 0.0003 | 0.0018 | 0.0005 | 0.0001 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0 | 0 |
Cytoplasm | 0.0024 | 0.0178 | 0.002 | 0.0006 | 0.0058 | 0.0024 | 0.0085 | 0.001 | 0.0005 | 0.0003 | 0.0023 | 0.0006 | 0.0028 | 0.0113 | 0.0026 | 0.0218 | 0.0016 | 0.0011 |
Cytoplasmic Foci | 0.0027 | 0.0006 | 0 | 0 | 0.0022 | 0.0002 | 0.0059 | 0.0002 | 0.0015 | 0 | 0.004 | 0.0015 | 0.0071 | 0.001 | 0.0055 | 0.0025 | 0.0073 | 0.0013 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0289 | 0.0588 | 0.0578 | 0.0733 | 0.1043 | 0.0176 | 0.0417 | 0.1587 | 0.1112 | 0.1351 | 0.0937 | 0.0941 | 0.0447 | 0.0726 | 0.0714 | 0.0713 | 0.0732 | 0.0511 |
Endosome | 0.0089 | 0.0053 | 0.0012 | 0.0023 | 0.0204 | 0.0027 | 0.0164 | 0.0031 | 0.0035 | 0.003 | 0.015 | 0.0043 | 0.0143 | 0.0026 | 0.0099 | 0.0336 | 0.0105 | 0.005 |
Golgi | 0.0023 | 0.0001 | 0 | 0.0001 | 0.0015 | 0.0001 | 0.0042 | 0.0002 | 0.0006 | 0.0002 | 0.0018 | 0.001 | 0.0048 | 0.0003 | 0.0023 | 0.0042 | 0.0058 | 0.0033 |
Lipid Particles | 0.0048 | 0.0003 | 0.0002 | 0.0002 | 0.0032 | 0.0003 | 0.0171 | 0.0018 | 0.0027 | 0.0004 | 0.0028 | 0.0052 | 0.0254 | 0.0014 | 0.0018 | 0.0307 | 0.042 | 0.0086 |
Mitochondria | 0.0014 | 0.0003 | 0.0003 | 0.0004 | 0.0037 | 0.0003 | 0.0021 | 0.0005 | 0.0007 | 0.0015 | 0.0011 | 0.0007 | 0.0054 | 0.0005 | 0.0021 | 0.0084 | 0.0007 | 0.0013 |
None | 0.0009 | 0.0002 | 0.0001 | 0.0001 | 0.0033 | 0.0004 | 0.0084 | 0.0001 | 0.0002 | 0.0002 | 0.0017 | 0.0001 | 0.0077 | 0.0003 | 0.0007 | 0.0008 | 0.0002 | 0.0001 |
Nuclear Periphery | 0.8395 | 0.7114 | 0.8131 | 0.8718 | 0.7562 | 0.8773 | 0.8072 | 0.7926 | 0.8363 | 0.8227 | 0.8303 | 0.8692 | 0.8229 | 0.788 | 0.8316 | 0.7123 | 0.7916 | 0.8761 |
Nucleolus | 0.0005 | 0.0012 | 0.0004 | 0.0001 | 0.0016 | 0.0004 | 0.0033 | 0.003 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0013 | 0.0006 | 0.0002 | 0.0011 | 0.0003 | 0.0002 |
Nucleus | 0.0637 | 0.1669 | 0.1057 | 0.0359 | 0.0672 | 0.0843 | 0.0124 | 0.0112 | 0.0075 | 0.0054 | 0.0193 | 0.0078 | 0.0302 | 0.0817 | 0.0304 | 0.0627 | 0.0099 | 0.0348 |
Peroxisomes | 0.0033 | 0 | 0 | 0 | 0.001 | 0 | 0.004 | 0 | 0.0055 | 0 | 0.001 | 0.0005 | 0.0036 | 0.0032 | 0.0045 | 0.0007 | 0.0297 | 0.0003 |
Punctate Nuclear | 0.0095 | 0.014 | 0.0006 | 0.0005 | 0.011 | 0.0035 | 0.008 | 0.0017 | 0.0009 | 0.0002 | 0.0099 | 0.0026 | 0.0103 | 0.0161 | 0.0035 | 0.0256 | 0.0148 | 0.0047 |
Vacuole | 0.0018 | 0.011 | 0.0015 | 0.0014 | 0.0031 | 0.0014 | 0.0033 | 0.0026 | 0.002 | 0.0018 | 0.002 | 0.001 | 0.0026 | 0.0034 | 0.0039 | 0.0057 | 0.0013 | 0.0012 |
Vacuole Periphery | 0.0135 | 0.0109 | 0.0168 | 0.0129 | 0.0097 | 0.0084 | 0.0202 | 0.0226 | 0.026 | 0.0287 | 0.0087 | 0.0102 | 0.0142 | 0.0139 | 0.0171 | 0.0139 | 0.0093 | 0.0098 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.0273 | 24.0345 | 21.4016 | 26.1838 | 25.0985 | 32.2454 | 26.4884 | 32.1474 | 24.6573 | 33.4479 |
Translational Efficiency | 1.1552 | 1.3745 | 1.2708 | 1.1016 | 1.1782 | 1.0806 | 1.35 | 0.9465 | 1.3458 | 1.0672 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1982 | 1173 | 2439 | 1088 | 1666 | 1964 | 1912 | 847 | 3648 | 3137 | 4351 | 1935 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 937.41 | 950.09 | 1179.30 | 1294.55 | 875.58 | 963.89 | 1114.06 | 1226.26 | 909.17 | 958.73 | 1150.63 | 1264.66 |
Standard Deviation | 128.65 | 170.43 | 160.03 | 234.83 | 129.42 | 133.96 | 160.49 | 267.50 | 132.63 | 148.80 | 163.47 | 251.95 |
Intensity Change Log 2 | — | 0.019384 | 0.331179 | 0.465699 | — | 0.138630 | 0.347516 | 0.485954 | — | 0.078205 | 0.339092 | 0.475516 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000105 | 0.000397 | 0.000420 | 0.018904 | 0.000252 | 0.000342 | 0.003111 | 0.026831 | 0.000172 | 0.000363 | 0.001603 | 0.022373 |
Bud Neck | 0.000190 | 0.000884 | 0.000330 | 0.009413 | 0.000186 | 0.000254 | 0.001803 | 0.010501 | 0.000188 | 0.000489 | 0.000977 | 0.009889 |
Bud Site | 0.000202 | 0.002340 | 0.000290 | 0.043962 | 0.000324 | 0.000594 | 0.005620 | 0.063509 | 0.000257 | 0.001247 | 0.002632 | 0.052519 |
Cell Periphery | 0.000087 | 0.000287 | 0.000088 | 0.011126 | 0.000189 | 0.000122 | 0.000684 | 0.006417 | 0.000134 | 0.000184 | 0.000350 | 0.009065 |
Cytoplasm | 0.004985 | 0.029285 | 0.007519 | 0.019725 | 0.010507 | 0.011348 | 0.033023 | 0.071733 | 0.007507 | 0.018055 | 0.018726 | 0.042490 |
Cytoplasmic Foci | 0.001082 | 0.009916 | 0.000283 | 0.019030 | 0.002255 | 0.005143 | 0.007510 | 0.040094 | 0.001618 | 0.006928 | 0.003459 | 0.028250 |
Eisosomes | 0.000004 | 0.000017 | 0.000004 | 0.001372 | 0.000113 | 0.000010 | 0.000174 | 0.001028 | 0.000054 | 0.000013 | 0.000079 | 0.001222 |
Endoplasmic Reticulum | 0.118253* | 0.266717* | 0.244837* | 0.682226* | 0.158754* | 0.215569* | 0.652880* | 0.456703* | 0.136749* | 0.234695* | 0.424147* | 0.583509* |
Endosome | 0.005141 | 0.029987 | 0.010360 | 0.021308 | 0.007183 | 0.013718 | 0.021666 | 0.037725 | 0.006073 | 0.019802 | 0.015328 | 0.028494 |
Golgi | 0.000588 | 0.005225 | 0.000276 | 0.014825 | 0.001178 | 0.003942 | 0.003673 | 0.021198 | 0.000857 | 0.004422 | 0.001769 | 0.017614 |
Lipid Particles | 0.009107 | 0.027967 | 0.003007 | 0.113621* | 0.018813 | 0.023300 | 0.037843 | 0.121314* | 0.013540 | 0.025045 | 0.018315 | 0.116988* |
Mitochondria | 0.002472 | 0.004086 | 0.000466 | 0.002420 | 0.002506 | 0.004232 | 0.001418 | 0.003412 | 0.002488 | 0.004178 | 0.000884 | 0.002854 |
Mitotic Spindle | 0.003118 | 0.005287 | 0.001905 | 0.008833 | 0.001193 | 0.003096 | 0.007268 | 0.027022 | 0.002239 | 0.003915 | 0.004261 | 0.016795 |
None | 0.002027 | 0.003730 | 0.001078 | 0.002265 | 0.002095 | 0.002136 | 0.001515 | 0.002575 | 0.002058 | 0.002732 | 0.001270 | 0.002401 |
Nuclear Periphery | 0.356983* | 0.119838* | 0.285000* | 0.000491 | 0.279311* | 0.216851* | 0.030684 | 0.001321 | 0.321511* | 0.180575* | 0.173244* | 0.000854 |
Nuclear Periphery Foci | 0.486673* | 0.471632* | 0.439877* | 0.013262 | 0.504274* | 0.484012* | 0.171434 | 0.035550 | 0.494712* | 0.479382* | 0.321912* | 0.023018 |
Nucleolus | 0.001138 | 0.002426 | 0.000113 | 0.000025 | 0.001571 | 0.000545 | 0.000092 | 0.000124 | 0.001335 | 0.001248 | 0.000104 | 0.000068 |
Nucleus | 0.004256 | 0.009702 | 0.002141 | 0.000894 | 0.005854 | 0.004527 | 0.005347 | 0.007774 | 0.004986 | 0.006462 | 0.003550 | 0.003905 |
Peroxisomes | 0.000176 | 0.000580 | 0.000026 | 0.002685 | 0.000441 | 0.000747 | 0.000791 | 0.004185 | 0.000297 | 0.000684 | 0.000362 | 0.003342 |
Vacuole | 0.002188 | 0.006792 | 0.001834 | 0.010414 | 0.001882 | 0.006483 | 0.012492 | 0.055827 | 0.002048 | 0.006598 | 0.006517 | 0.030292 |
Vacuole Periphery | 0.001225 | 0.002905 | 0.000146 | 0.003201 | 0.001120 | 0.003028 | 0.000972 | 0.005159 | 0.001177 | 0.002982 | 0.000509 | 0.004058 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.81 | -0.93 | -8.35 | -8.22 | -8.12 | -0.74 | -5.58 | -9.11 | -9.07 | -7.89 | -2.80 | -4.89 | -12.31 | -12.20 | -11.31 |
Bud Neck | -5.09 | -2.34 | -7.57 | -6.97 | -7.45 | -1.60 | -7.73 | -10.76 | -10.69 | -8.69 | -5.06 | -8.03 | -12.08 | -11.68 | -10.93 |
Bud Site | -3.68 | -0.94 | -12.20 | -11.47 | -12.17 | -1.47 | -6.66 | -12.68 | -12.63 | -11.34 | -4.13 | -6.67 | -17.56 | -17.18 | -16.56 |
Cell Periphery | -5.11 | -0.24 | -11.13 | -10.92 | -11.13 | 1.30 | -5.05 | -8.06 | -8.17 | -7.32 | -1.77 | -4.89 | -13.61 | -13.53 | -13.24 |
Cytoplasm | -8.83 | -2.80 | -8.08 | 2.82 | -6.99 | -0.50 | -9.76 | -11.91 | -11.85 | -7.80 | -7.53 | -9.74 | -13.59 | -9.58 | -9.43 |
Cytoplasmic Foci | -6.41 | 2.99 | -7.68 | -3.47 | -8.10 | -3.18 | -5.83 | -11.59 | -10.57 | -9.71 | -7.13 | -4.30 | -13.61 | -10.42 | -12.53 |
Eisosomes | -5.42 | 0.05 | -7.78 | -7.70 | -7.78 | 1.01 | -0.59 | -5.25 | -7.53 | -5.95 | 0.88 | -0.53 | -9.30 | -10.42 | -9.72 |
Endoplasmic Reticulum | -14.70 | -16.29 | -46.70* | -28.90 | -34.04* | -6.39 | -46.59* | -17.48 | -13.70 | 13.96 | -15.16 | -40.39 | -43.46 | -31.60 | -13.27 |
Endosome | -14.04 | -7.28 | -11.60 | 3.83 | -7.52 | -6.25 | -13.25 | -12.35 | -9.16 | -5.76 | -14.81 | -14.63 | -16.69 | -5.35 | -9.04 |
Golgi | -5.50 | 1.67 | -7.36 | -4.53 | -7.54 | -3.78 | -5.35 | -8.87 | -7.18 | -7.58 | -6.47 | -3.88 | -11.33 | -8.25 | -10.65 |
Lipid Particles | -7.78 | 5.72 | -17.07 | -13.34 | -18.31 | -1.85 | -6.55 | -14.34 | -13.68 | -11.11 | -6.86 | -3.39 | -22.24 | -19.29 | -20.99 |
Mitochondria | -1.97 | 5.32 | 0.05 | 1.66 | -2.86 | -2.67 | 2.48 | -0.67 | 0.96 | -1.73 | -3.30 | 5.66 | -0.45 | 1.98 | -3.18 |
Mitotic Spindle | -1.74 | 1.06 | -2.99 | -1.73 | -3.86 | -2.78 | -5.64 | -6.84 | -6.29 | -4.78 | -2.38 | -3.22 | -7.33 | -6.43 | -6.04 |
None | -2.53 | 1.86 | -0.18 | 1.51 | -1.23 | -0.05 | 0.95 | -0.31 | -0.26 | -0.85 | -1.42 | 2.02 | -0.34 | 0.59 | -1.48 |
Nuclear Periphery | 30.33* | 8.98 | 58.37* | 24.25* | 54.19* | 7.66 | 37.57* | 43.64* | 41.89* | 15.84 | 24.12 | 25.93 | 71.92 | 47.27* | 47.82* |
Nuclear Periphery Foci | 1.42 | 5.17 | 69.68* | 47.26* | 65.52* | 2.02 | 34.82* | 56.93* | 57.88* | 19.66* | 2.15 | 25.03 | 89.63 | 77.27* | 57.86* |
Nucleolus | -1.78 | 3.65 | 4.01 | 3.71 | 1.47 | 2.32 | 3.56 | 3.46 | 3.57 | -1.15 | 0.22 | 5.07 | 5.23 | 4.68 | 0.89 |
Nucleus | -3.51 | 2.51 | 4.56 | 6.09 | 3.19 | 1.06 | -0.06 | -3.27 | -4.11 | -3.39 | -1.80 | 1.66 | -0.76 | 0.96 | -2.28 |
Peroxisomes | -2.60 | 5.16 | -5.34 | -4.22 | -5.69 | -0.93 | -2.11 | -3.94 | -3.36 | -3.52 | -1.81 | -0.87 | -6.22 | -4.88 | -6.08 |
Vacuole | -5.12 | 0.53 | -6.28 | -2.67 | -6.58 | -4.81 | -9.00 | -12.48 | -11.58 | -10.08 | -6.53 | -7.89 | -13.29 | -11.43 | -11.11 |
Vacuole Periphery | -3.13 | 6.87 | -2.63 | -0.32 | -4.16 | -3.90 | 0.43 | -3.32 | -1.47 | -3.39 | -4.98 | 3.78 | -4.23 | -1.24 | -5.27 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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