Standard name
Human Ortholog
Description Endoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.15 0 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.27 0.15 0.22 0 0 0 0 0 0
Cytoplasm 0 0.05 0.07 0.09 0.13 0.13 0.12 0.12 0.11 0.16 0.17 0.15 0.13 0.17 0.18 0.2 0.28 0.24 0 0 0 0 0 0
Endoplasmic Reticulum 0.21 0.24 0.21 0.08 0.08 0.32 0.3 0.11 0.18 0.12 0.22 0.13 0.38 0.15 0.16 0 0 0 0.36 0.4 0.29 0.67 0.7 0.65
Endosome 0 0 0 0.07 0.08 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.16 0.17 0.07 0 0 0 0 0 0
Mitochondria 0.11 0 0 0.12 0.05 0.18 0.16 0.14 0.2 0.14 0.22 0.11 0 0 0 0 0 0.05 0 0 0 0 0 0
Nucleus 0 0 0.05 0.08 0 0 0 0.09 0.07 0.06 0.06 0.09 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.41 0.33 0.34 0.13 0 0.11 0.14 0.1 0.13 0.12 0.1 0.08 0.27 0.16 0.15 0.07 0.09 0.06 0.49 0.43 0.53 0.15 0.1 0.1
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.52 0.58 0.47 0.56 0.74 0.35 0.41 0.52 0.45 0.49 0.35 0.52 0.28 0.5 0.53 0.19 0.36 0.4 0.06 0.07 0.05 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 7 0 5 13 4 9 1 9 6 6 13 10 5 16 5 12 1 0 5 10 28 22
Bud 0 0 0 2 2 5 2 6 7 4 7 6 0 1 0 0 0 0 0 2 4 3 7 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 4 2
Bud Site 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 2 9 9 5 3 3 5 4 0 6 22 27 29 18 29 0 0 0 0 0 0
Cytoplasm 9 13 22 22 56 55 59 53 48 71 62 60 40 94 91 21 34 31 1 3 5 7 11 9
Endoplasmic Reticulum 44 64 62 19 35 135 149 52 76 53 78 50 118 82 77 1 1 4 90 136 119 282 520 373
Endosome 2 2 13 17 34 12 8 18 14 18 6 22 7 16 21 1 0 0 2 6 9 8 23 30
Golgi 0 2 1 0 0 0 0 0 0 0 0 0 0 1 1 17 20 9 0 5 3 3 7 7
Mitochondria 22 4 2 28 21 75 82 62 86 63 79 42 0 13 1 4 5 7 5 7 18 17 23 18
Nucleus 5 2 16 20 4 14 17 43 29 28 21 35 8 22 13 4 1 0 2 0 1 1 0 0
Nuclear Periphery 84 91 100 32 5 45 68 45 54 54 36 31 83 85 72 7 11 8 123 145 217 62 75 60
Nucleolus 0 3 5 1 0 2 3 4 0 1 4 2 1 1 3 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
SpindlePole 0 0 0 2 1 0 0 1 0 0 0 0 0 3 1 0 1 0 1 0 1 1 3 2
Vac/Vac Membrane 108 157 138 135 320 146 203 237 190 216 126 204 87 270 260 20 44 51 15 24 18 16 32 36
Unique Cell Count 207 272 295 240 431 418 500 458 420 442 359 390 310 541 494 107 121 129 250 337 410 423 746 578
Labelled Cell Count 274 339 366 280 492 511 601 534 508 522 429 458 363 620 572 120 140 151 250 337 410 423 746 578


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 10.8 8.3 7.8 8.5 7.5 7.2 7.7 7.3 7.4 6.7 7.3 8.9 8.9 9.6 14.1 14.1 14.0 9.0 9.4 10.0
Std Deviation (1e-4) 1.1 1.7 1.2 1.4 1.4 1.3 1.3 1.3 1.5 1.6 1.4 1.5 1.4 1.5 1.7 2.7 2.7 2.7 1.8 1.6 1.8
Intensity Change (Log2) -0.08 0.04 -0.15 -0.21 -0.1 -0.19 -0.17 -0.31 -0.19 0.1 0.11 0.22 0.77 0.77 0.76 0.13 0.18 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0.6 0 -0.4 0 -0.3 -0.6 -0.8 1.3 -0.5 0 4.8 0 3.2
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 3.5 4.1 9.3 6.8 8.4
Cytoplasm 0.7 2.4 2.4 2.0 1.8 1.8 3.4 3.7 3.2 2.2 4.0 4.3 3.5 5.6 4.7
Endoplasmic Reticulum -4.2 -5.0 3.3 2.7 -3.6 -1.0 -3.3 0.2 -2.9 4.6 -2.1 -1.9 -4.9 -5.2 -4.7
Endosome 1.3 1.9 -1.1 -2.4 -0.3 -0.7 -0.2 -2.1 0.7 -1.5 -1.1 -0.1 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 6.8 0
Mitochondria 5.5 3.2 7.3 7.0 6.2 7.9 6.4 8.2 5.3 0 1.8 0 0 0 0
Nucleus 1.3 -3.6 -1.4 -1.4 2.0 0.8 0.5 0.2 1.8 -1.8 -0.9 -2.0 -0.7 0 0
Nuclear Periphery -5.5 -12.3 -7.6 -6.8 -8.2 -6.7 -7.1 -7.5 -8.6 -1.9 -6.1 -6.4 -5.5 -5.2 -6.0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.2 7.5 -3.2 -1.7 1.3 -0.4 0.6 -3.0 1.4 -4.8 0.9 1.6 -5.1 -1.9 -1.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.976 5.4095 5.4274 5.2548 4.3895 5.2397 5.6348 5.9462 5.4248 5.3242 4.784 5.3946 7.3751 6.8241 6.3947 6.5157 5.7732 6.6898
Actin 0.0124 0.0002 0.0002 0.0003 0.0029 0.0003 0.0341 0.0003 0.0006 0.0004 0.0031 0.0008 0.0018 0.0007 0.0088 0.0017 0.0015 0.0007
Bud 0.0001 0.0001 0 0 0.0003 0 0.0002 0 0 0 0.0002 0 0.0001 0.0014 0.0003 0.0014 0 0
Bud Neck 0.0019 0.0003 0.0001 0 0.0015 0.0002 0.0017 0.0002 0.0001 0 0.0009 0.0001 0.0002 0.0001 0.0009 0.0002 0.0001 0.0002
Bud Periphery 0.0001 0.0001 0 0 0.0005 0 0.0003 0 0 0.0001 0.0001 0 0.0002 0.0005 0.0006 0.0005 0 0
Bud Site 0.0011 0.0004 0 0 0.0006 0 0.0007 0.0001 0 0 0.0014 0 0.0003 0.0003 0.0018 0.0005 0.0001 0.0001
Cell Periphery 0.0002 0.0001 0 0 0.0001 0 0.0003 0.0001 0.0001 0.0001 0 0 0.0001 0.0001 0.0001 0.0004 0 0
Cytoplasm 0.0024 0.0178 0.002 0.0006 0.0058 0.0024 0.0085 0.001 0.0005 0.0003 0.0023 0.0006 0.0028 0.0113 0.0026 0.0218 0.0016 0.0011
Cytoplasmic Foci 0.0027 0.0006 0 0 0.0022 0.0002 0.0059 0.0002 0.0015 0 0.004 0.0015 0.0071 0.001 0.0055 0.0025 0.0073 0.0013
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0 0 0 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0289 0.0588 0.0578 0.0733 0.1043 0.0176 0.0417 0.1587 0.1112 0.1351 0.0937 0.0941 0.0447 0.0726 0.0714 0.0713 0.0732 0.0511
Endosome 0.0089 0.0053 0.0012 0.0023 0.0204 0.0027 0.0164 0.0031 0.0035 0.003 0.015 0.0043 0.0143 0.0026 0.0099 0.0336 0.0105 0.005
Golgi 0.0023 0.0001 0 0.0001 0.0015 0.0001 0.0042 0.0002 0.0006 0.0002 0.0018 0.001 0.0048 0.0003 0.0023 0.0042 0.0058 0.0033
Lipid Particles 0.0048 0.0003 0.0002 0.0002 0.0032 0.0003 0.0171 0.0018 0.0027 0.0004 0.0028 0.0052 0.0254 0.0014 0.0018 0.0307 0.042 0.0086
Mitochondria 0.0014 0.0003 0.0003 0.0004 0.0037 0.0003 0.0021 0.0005 0.0007 0.0015 0.0011 0.0007 0.0054 0.0005 0.0021 0.0084 0.0007 0.0013
None 0.0009 0.0002 0.0001 0.0001 0.0033 0.0004 0.0084 0.0001 0.0002 0.0002 0.0017 0.0001 0.0077 0.0003 0.0007 0.0008 0.0002 0.0001
Nuclear Periphery 0.8395 0.7114 0.8131 0.8718 0.7562 0.8773 0.8072 0.7926 0.8363 0.8227 0.8303 0.8692 0.8229 0.788 0.8316 0.7123 0.7916 0.8761
Nucleolus 0.0005 0.0012 0.0004 0.0001 0.0016 0.0004 0.0033 0.003 0.0001 0 0.0009 0.0001 0.0013 0.0006 0.0002 0.0011 0.0003 0.0002
Nucleus 0.0637 0.1669 0.1057 0.0359 0.0672 0.0843 0.0124 0.0112 0.0075 0.0054 0.0193 0.0078 0.0302 0.0817 0.0304 0.0627 0.0099 0.0348
Peroxisomes 0.0033 0 0 0 0.001 0 0.004 0 0.0055 0 0.001 0.0005 0.0036 0.0032 0.0045 0.0007 0.0297 0.0003
Punctate Nuclear 0.0095 0.014 0.0006 0.0005 0.011 0.0035 0.008 0.0017 0.0009 0.0002 0.0099 0.0026 0.0103 0.0161 0.0035 0.0256 0.0148 0.0047
Vacuole 0.0018 0.011 0.0015 0.0014 0.0031 0.0014 0.0033 0.0026 0.002 0.0018 0.002 0.001 0.0026 0.0034 0.0039 0.0057 0.0013 0.0012
Vacuole Periphery 0.0135 0.0109 0.0168 0.0129 0.0097 0.0084 0.0202 0.0226 0.026 0.0287 0.0087 0.0102 0.0142 0.0139 0.0171 0.0139 0.0093 0.0098

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.0273 24.0345 21.4016 26.1838 25.0985 32.2454 26.4884 32.1474 24.6573 33.4479
Translational Efficiency 1.1552 1.3745 1.2708 1.1016 1.1782 1.0806 1.35 0.9465 1.3458 1.0672

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Endoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p
Localization
Cell Percentages nuclear periphery (25%), ER (19%), mixed (56%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Dfm1

Dfm1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dfm1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available