Standard name
Human Ortholog
Description Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0.05 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.37 0.44 0.53 0.52 0.49 0.43 0.35 0.35 0.28 0.28 0.19 0.22 0.45 0.67 0.7 0.41 0.35 0.28 0.17 0.35 0.24 0.42 0.34 0.27
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.05 0.09 0 0 0 0 0 0
Endosome 0.26 0 0 0 0.05 0 0 0 0 0 0 0 0.14 0.07 0 0.22 0.26 0.26 0.17 0.06 0.09 0 0.05 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0.46 0.41 0.49 0.35 0.63 0.69 0.65 0.76 0.8 0.82 0.83 0.17 0.09 0.25 0 0 0.1 0.09 0.1 0.24 0.05 0.07 0.09
Nucleus 0.06 0 0 0 0 0 0.07 0.07 0.09 0.11 0.08 0.09 0.07 0 0.05 0.13 0.08 0.18 0.06 0.07 0.05 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.11 0 0 0.06 0.17 0 0.08 0.07 0 0.05 0.06 0.06 0 0 0 0.05 0.07 0.06 0.28 0.16 0.14 0.18 0.19 0.24
Vac/Vac Membrane 0.25 0.21 0 0 0 0 0 0 0 0 0 0 0.21 0.17 0.11 0.26 0.41 0.28 0.11 0.16 0.13 0.16 0.2 0.12
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 3
Bud 0 2 1 10 4 13 13 20 12 5 14 13 0 0 3 0 0 2 0 1 0 1 1 5
Bud Neck 1 1 10 1 5 2 4 5 2 1 2 2 0 0 0 0 0 3 0 1 0 1 1 2
Bud Site 0 0 1 2 3 2 10 17 10 8 4 18 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 65 62 86 105 119 147 151 180 83 100 46 72 49 78 105 71 60 57 47 62 52 20 41 49
Endoplasmic Reticulum 3 0 5 0 0 0 0 0 0 0 0 1 0 1 1 13 9 18 2 0 2 0 1 1
Endosome 47 6 0 0 12 2 3 0 0 0 0 0 15 8 6 38 44 53 49 10 19 1 6 12
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 0 0 0 0 1
Mitochondria 12 65 66 98 85 212 301 331 224 288 196 276 18 10 37 7 5 20 26 18 51 2 8 16
Nucleus 10 1 3 4 8 11 29 36 26 38 20 31 8 5 7 23 14 37 16 11 10 2 4 7
Nuclear Periphery 1 0 0 0 0 0 1 0 1 1 1 1 0 0 0 0 0 0 4 0 1 0 0 0
Nucleolus 2 0 0 1 0 0 1 6 3 0 1 6 0 0 0 1 0 0 5 2 2 0 1 1
Peroxisomes 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2
SpindlePole 20 2 3 12 41 14 34 34 11 19 15 19 1 1 2 9 12 13 81 28 30 8 22 43
Vac/Vac Membrane 45 29 1 3 10 10 11 7 8 5 5 5 23 20 17 45 70 57 31 28 27 7 23 22
Unique Cell Count 178 140 162 202 244 338 437 508 295 361 238 332 109 116 151 173 171 205 286 178 218 48 121 181
Labelled Cell Count 207 168 177 236 287 413 558 636 380 465 304 444 114 123 179 207 215 261 286 178 218 48 121 181


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 3.1 3.0 3.3 3.6 3.2 2.9 3.0 2.7 2.6 2.7 2.3 4.1 4.2 3.7 5.4 5.6 5.8 3.9 3.9 5.1
Std Deviation (1e-4) 0.8 0.8 0.8 1.7 1.8 1.5 0.9 1.3 1.2 1.1 1.3 1.0 1.5 1.3 1.3 1.3 1.4 1.5 1.5 1.2 2.1
Intensity Change (Log2) – – – 0.11 0.27 0.07 -0.09 -0.02 -0.18 -0.23 -0.14 -0.38 0.43 0.48 0.28 0.84 0.89 0.94 0.36 0.35 0.76


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 6.7 9.0 7.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0272 0.1953 -0.1966 -0.2217 0.0367 -0.1442 0.2517 0.3631 0.3736 0.8076 0.8221 0.2978 1.0816 1.0265 1.2407 1.1387 0.6213 1.115
Actin 0.0266 0.0003 0.0076 0.0016 0.0056 0.0019 0.0105 0.0005 0.0081 0.0293 0.0055 0.0009 0.0149 0.0005 0.0166 0.0011 0.0003 0.0004
Bud 0.0008 0.0004 0.0003 0.0003 0.0009 0.0001 0.0006 0.0001 0.0005 0.001 0.0008 0.0002 0.0003 0.0047 0.0071 0.0017 0.0001 0.0004
Bud Neck 0.0123 0.0039 0.0026 0.0437 0.025 0.0103 0.0061 0.0006 0.003 0.019 0.0027 0.0034 0.0022 0.0007 0.0015 0.0005 0.0011 0.002
Bud Periphery 0.0024 0.0008 0.0005 0.0006 0.002 0.0003 0.001 0.0001 0.001 0.0017 0.0018 0.0011 0.0004 0.0007 0.0017 0.0079 0.0001 0.0001
Bud Site 0.0043 0.0106 0.0022 0.0021 0.0028 0.0003 0.0085 0.0008 0.0057 0.0149 0.001 0.0002 0.0102 0.0055 0.0018 0.0002 0.0001 0.0002
Cell Periphery 0.0008 0.0004 0.0002 0.0007 0.0004 0.0001 0.0002 0.0001 0.0002 0.0005 0.0001 0 0.0003 0.0004 0.0003 0.0001 0.0001 0.0001
Cytoplasm 0.161 0.1857 0.1667 0.2084 0.122 0.1928 0.1064 0.1803 0.1208 0.0747 0.1421 0.1223 0.1426 0.1472 0.1241 0.0679 0.1369 0.1547
Cytoplasmic Foci 0.0463 0.0298 0.0488 0.0246 0.0511 0.0344 0.0398 0.0291 0.0246 0.0186 0.0287 0.0097 0.0328 0.022 0.0205 0.0082 0.021 0.0407
Eisosomes 0.001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0002 0.0001 0.0002 0.0005 0.0002 0 0.0004 0.0001 0.0002 0.0002 0.0001 0.0001
Endoplasmic Reticulum 0.0091 0.0067 0.0098 0.0064 0.0067 0.0076 0.005 0.003 0.0045 0.0033 0.0067 0.002 0.0051 0.0099 0.005 0.0042 0.0026 0.0025
Endosome 0.0392 0.0122 0.0208 0.0211 0.0351 0.0158 0.0305 0.0074 0.0144 0.0155 0.0272 0.0038 0.0102 0.0071 0.0079 0.0042 0.0169 0.0052
Golgi 0.0074 0.0006 0.0015 0.0005 0.0061 0.001 0.0057 0.0003 0.0037 0.0082 0.0061 0.0003 0.0043 0.0004 0.0013 0.0003 0.0028 0.0002
Lipid Particles 0.0292 0.0055 0.0107 0.0014 0.0302 0.0056 0.0179 0.0011 0.0098 0.0256 0.0316 0.0011 0.0178 0.0014 0.0029 0.0031 0.0044 0.0029
Mitochondria 0.0148 0.0007 0.002 0.0012 0.0039 0.0049 0.0043 0.0004 0.0057 0.0193 0.0559 0.0009 0.0022 0.001 0.0113 0.0012 0.0228 0.0007
None 0.2734 0.4792 0.3226 0.2568 0.3881 0.2529 0.3915 0.5156 0.3901 0.3481 0.3177 0.3882 0.4264 0.5439 0.5951 0.6529 0.5571 0.5275
Nuclear Periphery 0.0244 0.0132 0.025 0.0325 0.0193 0.0207 0.0254 0.0063 0.0221 0.0172 0.0319 0.0108 0.0143 0.0083 0.0103 0.015 0.0038 0.0064
Nucleolus 0.0074 0.0131 0.0066 0.0086 0.0062 0.0101 0.0079 0.0056 0.0082 0.0078 0.0057 0.0067 0.0064 0.0046 0.0031 0.0028 0.0035 0.0044
Nucleus 0.224 0.1655 0.2041 0.2553 0.1457 0.2863 0.1963 0.1402 0.2041 0.2191 0.1937 0.3166 0.1525 0.1467 0.1248 0.1529 0.116 0.1556
Peroxisomes 0.0174 0.0006 0.0084 0.0006 0.0163 0.0027 0.0139 0.0007 0.005 0.0128 0.0214 0.0005 0.0378 0.001 0.0024 0.0009 0.0013 0.0004
Punctate Nuclear 0.0889 0.0666 0.1541 0.1271 0.1263 0.1483 0.1235 0.1059 0.1648 0.1585 0.1115 0.1302 0.1163 0.0911 0.0597 0.0729 0.1061 0.0943
Vacuole 0.0068 0.0037 0.0046 0.0054 0.0044 0.0028 0.0036 0.0016 0.0025 0.0031 0.0037 0.0008 0.0024 0.0025 0.002 0.0014 0.0018 0.0012
Vacuole Periphery 0.0025 0.0004 0.0008 0.0008 0.0018 0.0012 0.0013 0.0002 0.0007 0.0012 0.004 0.0002 0.0004 0.0004 0.0005 0.0005 0.0012 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.188 12.1007 5.9006 10.4934 8.2738 6.9577 9.0888 11.089 13.1162 11.2884
Translational Efficiency 0.4566 0.2421 0.6114 0.4046 0.3513 0.4118 0.4451 0.3779 0.3488 0.3791

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
95 1494 196 573 1783 1639 165 82 1878 3133 361 655

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 649.39 676.48 990.12 792.97 634.21 699.24 974.12 895.11 634.98 688.39 982.81 805.76
Standard Deviation 65.30 102.76 127.99 105.65 82.65 84.45 110.02 106.42 81.93 94.32 120.37 111.01
Intensity Change Log 2 — 0.058962 0.608518 0.288181 — 0.140827 0.619139 0.497104 — 0.099991 0.613776 0.395188

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000038 0.000181 0.002873 0.000708 0.000081 0.000592 0.002542 0.002285 0.000079 0.000396 0.002722 0.000905
Bud Neck 0.008156 0.016004 0.010870 0.009646 0.008396 0.021308 0.009046 0.005676 0.008383 0.018779 0.010036 0.009149
Bud Site 0.001040 0.003149 0.026154 0.022288 0.001419 0.008661 0.010989 0.004231 0.001400 0.006033 0.019222 0.020028
Cell Periphery 0.000128 0.000116 0.000346 0.000315 0.000103 0.000134 0.000212 0.000309 0.000105 0.000126 0.000285 0.000314
Cytoplasm 0.393970* 0.353249* 0.121887* 0.329667* 0.363607* 0.326969* 0.057492 0.143819* 0.365143* 0.339501* 0.092454 0.306400*
Cytoplasmic Foci 0.257679* 0.198898* 0.005091 0.028763 0.185205* 0.224563* 0.001750 0.016165 0.188871* 0.212324* 0.003564 0.027186
Eisosomes 0.000150 0.000107 0.000122 0.000067 0.000102 0.000111 0.000138 0.000171 0.000104 0.000109 0.000129 0.000080
Endoplasmic Reticulum 0.000463 0.001557 0.021937 0.002795 0.001253 0.001251 0.022714 0.032978 0.001213 0.001397 0.022292 0.006573
Endosome 0.008733 0.012691 0.004190 0.015553 0.004980 0.014692 0.003648 0.005105 0.005170 0.013738 0.003942 0.014245
Golgi 0.000486 0.002450 0.000328 0.006466 0.001121 0.002926 0.000254 0.000250 0.001089 0.002699 0.000294 0.005688
Lipid Particles 0.016675 0.024547 0.003648 0.005624 0.028232 0.023056 0.004002 0.008269 0.027648 0.023767 0.003810 0.005955
Mitochondria 0.001571 0.014683 0.001766 0.015317 0.009202 0.020401 0.001576 0.001777 0.008816 0.017674 0.001679 0.013622
Mitotic Spindle 0.000069 0.008188 0.014942 0.064979 0.002961 0.008611 0.007093 0.013882 0.002814 0.008409 0.011355 0.058582
None 0.004877 0.011068 0.005920 0.016055 0.009578 0.005115 0.012164 0.012715 0.009340 0.007954 0.008774 0.015637
Nuclear Periphery 0.001015 0.003187 0.002181 0.002254 0.002861 0.002267 0.003519 0.006547 0.002768 0.002705 0.002793 0.002792
Nuclear Periphery Foci 0.000676 0.002348 0.015166 0.001334 0.001730 0.001193 0.009093 0.009668 0.001677 0.001744 0.012391 0.002378
Nucleolus 0.003029 0.010694 0.004822 0.002911 0.006547 0.005877 0.009779 0.005241 0.006370 0.008174 0.007088 0.003202
Nucleus 0.259595* 0.297224* 0.707074* 0.387454* 0.352849* 0.283069* 0.804821* 0.617318* 0.348132* 0.289819* 0.751751* 0.416231*
Peroxisomes 0.003509 0.012125 0.000874 0.001662 0.007419 0.016410 0.001177 0.002046 0.007221 0.014366 0.001013 0.001711
Vacuole 0.037428 0.025915 0.049341 0.078700 0.011898 0.031273 0.037542 0.111096 0.013189 0.028718 0.043948 0.082755
Vacuole Periphery 0.000714 0.001619 0.000466 0.007443 0.000456 0.001521 0.000448 0.000452 0.000469 0.001568 0.000458 0.006568

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -9.31 -11.07 -7.50 -5.87 8.46 -1.44 -9.62 -6.60 -3.57 2.96 -1.81 -14.56 -8.92 -3.24 10.12
Bud Neck -2.62 -1.27 -0.54 3.62 1.12 -7.83 -3.25 -1.93 3.82 0.90 -8.12 -2.77 -1.17 6.45 1.95
Bud Site -3.52 -3.32 -5.41 -4.43 0.71 -5.47 -3.17 -4.26 -0.89 -0.20 -6.08 -4.22 -5.76 -3.54 0.16
Cell Periphery 0.69 -8.88 -3.19 -4.25 6.01 -1.44 -7.32 -5.13 -3.53 0.44 -1.79 -12.23 -5.71 -4.20 5.31
Cytoplasm 1.93 11.13 5.88 8.84 -9.74 3.73 38.49 14.66 12.79 -3.81 3.27 34.10 12.58 11.01 -13.17
Cytoplasmic Foci 2.67 11.71 11.04 31.33 -6.30 -5.13 39.74 33.08 36.07 -3.59 -3.43 40.90 35.22 46.21 -7.48
Eisosomes 2.21 -1.32 2.44 0.75 5.26 -0.98 -5.29 -4.36 -4.04 -0.38 -0.63 -7.17 -1.54 -1.09 5.89
Endoplasmic Reticulum -7.17 -14.69 -13.48 -11.31 10.17 -0.13 -12.69 -6.71 -6.71 1.01 -1.71 -18.98 -12.61 -12.37 11.53
Endosome -1.80 1.03 0.66 3.92 -0.52 -8.50 -0.27 -1.98 5.79 -1.35 -11.20 -1.38 -2.03 5.24 -1.15
Golgi -4.93 -0.70 -1.32 0.19 -1.23 -5.12 4.37 5.36 8.55 1.67 -5.64 3.89 -0.73 0.63 -1.27
Lipid Particles -1.69 2.17 2.52 11.93 1.64 2.80 14.01 12.52 10.51 -0.73 2.49 14.57 14.28 15.61 1.34
Mitochondria -9.01 -0.58 -2.28 3.76 -2.14 -5.39 7.16 7.43 10.83 1.56 -5.68 7.12 1.98 6.23 -2.10
Mitotic Spindle -6.93 -2.29 -6.49 -4.57 -3.40 -3.62 -2.78 -2.28 -1.92 -1.57 -5.35 -2.86 -6.35 -5.14 -4.03
None -3.75 0.07 -1.79 1.53 -2.39 5.60 1.71 2.32 0.04 0.75 2.16 3.56 1.15 -0.14 -1.52
Nuclear Periphery -4.88 -6.35 -9.55 -3.58 -2.70 1.65 -5.44 -5.09 -5.88 -1.34 0.14 -4.92 -6.14 -6.90 -1.14
Nuclear Periphery Foci -4.84 -4.56 -5.66 0.52 4.22 2.49 -3.43 -3.69 -4.01 0.40 0.04 -5.41 -3.58 -3.70 4.86
Nucleolus -6.02 -3.03 0.51 8.69 3.64 2.97 -2.31 3.95 1.92 3.50 -0.91 -2.45 7.00 8.81 5.20
Nucleus -1.80 -14.52 -8.32 -11.32 8.81 7.48 -20.30 -4.56 -6.44 6.16 7.24 -24.73 -9.31 -13.68 12.64
Peroxisomes -8.01 3.24 3.66 12.93 1.39 -7.10 11.27 11.24 14.31 0.52 -7.76 11.94 12.20 19.20 1.55
Vacuole 1.08 -4.07 -9.94 -14.64 -5.99 -10.20 -6.41 -7.98 -7.30 -5.37 -10.97 -10.31 -17.88 -16.09 -8.18
Vacuole Periphery -2.61 0.48 -2.08 -1.15 -2.27 -7.48 -0.32 1.96 8.05 1.77 -7.81 -1.48 -2.39 -1.03 -2.30
Standard name
Human Ortholog
Description Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids
Localization
Cell Percentages nucleus (39%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Aro80

Aro80


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aro80-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available