Standard name | |
---|---|
Human Ortholog | |
Description | Sorting nexin; involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 4 | 4 | 8 | 10 | 7 | 12 | 13 | – | 5 | 8 | 1 | 9 | 1 | 6 | 3 | 11 | 3 | 13 | 1 | 2 | 2 | 0 | 1 | 3 |
Bud | 5 | 2 | 4 | 3 | 6 | 8 | 12 | – | 16 | 16 | 14 | 13 | 1 | 3 | 2 | 1 | 1 | 5 | 0 | 2 | 0 | 0 | 1 | 1 |
Bud Neck | 1 | 0 | 0 | 0 | 0 | 2 | 3 | – | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 2 | 1 |
Bud Site | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 3 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 30 | 33 | 16 | 32 | 32 | 57 | 66 | – | 44 | 81 | 46 | 86 | 26 | 55 | 49 | 51 | 55 | 63 | 11 | 11 | 9 | 4 | 34 | 31 |
Endoplasmic Reticulum | 2 | 0 | 0 | 4 | 3 | 6 | 8 | – | 1 | 3 | 4 | 8 | 3 | 1 | 3 | 11 | 10 | 15 | 0 | 0 | 1 | 0 | 2 | 1 |
Endosome | 105 | 93 | 35 | 52 | 96 | 133 | 140 | – | 77 | 114 | 55 | 81 | 65 | 124 | 189 | 204 | 226 | 220 | 121 | 113 | 71 | 79 | 145 | 209 |
Golgi | 0 | 0 | 2 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 7 | 4 | 6 | 3 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 12 | 1 | 0 | 0 | 1 | 9 | 17 | – | 33 | 28 | 89 | 76 | 0 | 0 | 0 | 7 | 6 | 9 | 3 | 2 | 11 | 4 | 8 | 9 |
Nucleus | 0 | 1 | 0 | 0 | 1 | 1 | 3 | – | 1 | 5 | 1 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Nuclear Periphery | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 |
Nucleolus | 10 | 8 | 1 | 3 | 4 | 3 | 6 | – | 2 | 2 | 9 | 6 | 3 | 6 | 2 | 17 | 15 | 21 | 0 | 0 | 0 | 1 | 1 | 1 |
Peroxisomes | 4 | 0 | 0 | 1 | 0 | 2 | 5 | – | 1 | 4 | 3 | 5 | 0 | 2 | 9 | 5 | 6 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 52 | 40 | 31 | 36 | 45 | 57 | 94 | – | 40 | 87 | 31 | 67 | 33 | 75 | 95 | 154 | 135 | 129 | 35 | 21 | 14 | 15 | 32 | 39 |
Vac/Vac Membrane | 8 | 32 | 9 | 9 | 17 | 19 | 18 | – | 18 | 23 | 15 | 18 | 21 | 13 | 19 | 62 | 52 | 59 | 9 | 10 | 6 | 6 | 19 | 17 |
Unique Cell Count | 173 | 158 | 82 | 114 | 170 | 237 | 305 | 182 | 304 | 206 | 287 | 119 | 222 | 265 | 378 | 361 | 352 | 192 | 171 | 121 | 117 | 260 | 326 | |
Labelled Cell Count | 236 | 214 | 107 | 150 | 212 | 310 | 386 | 239 | 376 | 273 | 373 | 155 | 285 | 373 | 534 | 514 | 542 | 192 | 171 | 121 | 117 | 260 | 326 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.9 | 7.5 | 6.8 | 6.8 | 7.5 | 6.7 | 6.8 | – | 5.7 | 6.0 | 5.2 | 5.6 | 7.2 | 7.4 | 7.4 | 10.3 | 9.8 | 9.3 | 6.4 | 6.8 | 7.0 |
Std Deviation (1e-4) | 0.7 | 0.8 | 1.1 | 1.3 | 1.3 | 1.0 | 1.2 | – | 1.0 | 1.0 | 0.9 | 0.9 | 1.3 | 1.0 | 1.1 | 2.3 | 1.8 | 1.9 | 1.3 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | -0.01 | 0.13 | -0.03 | -0.01 | – | -0.26 | -0.2 | -0.39 | -0.29 | 0.08 | 0.11 | 0.12 | 0.58 | 0.52 | 0.44 | -0.09 | 0.0 | 0.04 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -0.2 | 0 | -1.5 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 1.1 | 0 | 0.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.4 | -0.1 | 0.8 | 0.4 | – | 0.8 | 1.3 | 0.5 | 1.9 | 0.4 | 1.0 | -0.2 | -1.4 | -1.0 | -0.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.4 | 2.1 | 2.1 | 0.5 | – | -0.1 | -0.9 | -2.6 | -2.5 | 1.7 | 2.0 | 4.7 | 1.9 | 3.3 | 3.3 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 4.1 | 2.9 | 7.2 | 5.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.9 | -1.8 | -2.4 | -1.2 | – | -2.7 | -1.6 | -4.2 | -2.6 | -1.5 | -0.7 | -0.3 | 0.5 | -0.1 | -0.2 |
Vacuole | -0.7 | -0.2 | -0.8 | -1.6 | – | -0.3 | -1.0 | -1.0 | -1.4 | 1.3 | -1.5 | -1.1 | 1.2 | 0.8 | 1.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0638 | 0.7379 | 0.4164 | 0.1558 | 0.4066 | 0.8236 | 1.2656 | 2.1577 | 1.7655 | 1.1998 | 1.6995 | 1.7233 | 0.1915 | 1.1156 | 0.7899 | 0.7547 | 0.2516 | 0.5947 |
Actin | 0.0336 | 0.0003 | 0.0271 | 0.0051 | 0.018 | 0.0007 | 0.0253 | 0.0002 | 0.0064 | 0.0018 | 0.0045 | 0.0002 | 0.0147 | 0.0001 | 0.0141 | 0.0137 | 0 | 0.0063 |
Bud | 0.0008 | 0.0001 | 0.0019 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0004 | 0.0006 | 0.0003 | 0.0008 | 0.0001 | 0.0007 | 0.0003 | 0.0004 | 0.0011 | 0 | 0.0001 |
Bud Neck | 0.0022 | 0.0002 | 0.001 | 0.0033 | 0.0005 | 0.001 | 0.0055 | 0.0007 | 0.001 | 0.0003 | 0.0008 | 0.0004 | 0.0026 | 0.0003 | 0.001 | 0.0042 | 0.0001 | 0.0159 |
Bud Periphery | 0.0011 | 0.0001 | 0.0009 | 0.0003 | 0.0004 | 0.0003 | 0.0012 | 0.0008 | 0.0006 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0005 | 0.0005 | 0.0008 | 0 | 0.0001 |
Bud Site | 0.0057 | 0.001 | 0.0031 | 0.0017 | 0.001 | 0.0003 | 0.0027 | 0.0024 | 0.0015 | 0.0003 | 0.0015 | 0.0001 | 0.007 | 0.0014 | 0.0029 | 0.1317 | 0.0001 | 0.0002 |
Cell Periphery | 0.0004 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0002 | 0.0009 | 0 | 0 |
Cytoplasm | 0.0616 | 0.1665 | 0.1491 | 0.1161 | 0.1497 | 0.2538 | 0.1442 | 0.1746 | 0.1415 | 0.1413 | 0.2551 | 0.1998 | 0.1815 | 0.2499 | 0.1923 | 0.1279 | 0.2277 | 0.31 |
Cytoplasmic Foci | 0.2095 | 0.3188 | 0.2334 | 0.1729 | 0.2277 | 0.1867 | 0.1951 | 0.2953 | 0.2544 | 0.2362 | 0.2202 | 0.2745 | 0.2076 | 0.2392 | 0.2337 | 0.1708 | 0.1698 | 0.2124 |
Eisosomes | 0.0006 | 0 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0077 | 0.0049 | 0.0057 | 0.0048 | 0.002 | 0.0048 | 0.0091 | 0.0025 | 0.0035 | 0.0037 | 0.0027 | 0.0027 | 0.0059 | 0.0082 | 0.0048 | 0.0012 | 0.0007 | 0.0051 |
Endosome | 0.4904 | 0.4023 | 0.4157 | 0.518 | 0.3483 | 0.4347 | 0.4368 | 0.4068 | 0.4325 | 0.447 | 0.2924 | 0.4187 | 0.3674 | 0.3818 | 0.4316 | 0.2958 | 0.5346 | 0.3596 |
Golgi | 0.0241 | 0.0124 | 0.0322 | 0.0251 | 0.0375 | 0.0164 | 0.0189 | 0.0115 | 0.0176 | 0.0133 | 0.028 | 0.0133 | 0.0123 | 0.0101 | 0.0211 | 0.0384 | 0.0109 | 0.0107 |
Lipid Particles | 0.0523 | 0.0223 | 0.0292 | 0.0108 | 0.043 | 0.0201 | 0.0494 | 0.0187 | 0.02 | 0.0057 | 0.0173 | 0.013 | 0.0371 | 0.0143 | 0.0264 | 0.0789 | 0.0379 | 0.0133 |
Mitochondria | 0.0093 | 0.0123 | 0.005 | 0.0345 | 0.0713 | 0.0242 | 0.0068 | 0.0008 | 0.0072 | 0.0057 | 0.0036 | 0.0026 | 0.0077 | 0.0069 | 0.0083 | 0.0035 | 0.0004 | 0.0008 |
None | 0.0467 | 0.0361 | 0.0505 | 0.0579 | 0.0464 | 0.0339 | 0.0658 | 0.0574 | 0.0694 | 0.0862 | 0.1117 | 0.0512 | 0.1112 | 0.0562 | 0.0324 | 0.002 | 0.001 | 0.04 |
Nuclear Periphery | 0.0035 | 0.001 | 0.0023 | 0.0012 | 0.0004 | 0.0012 | 0.0041 | 0.001 | 0.0014 | 0.006 | 0.0013 | 0.0009 | 0.004 | 0.0027 | 0.0014 | 0.0006 | 0.0004 | 0.003 |
Nucleolus | 0.0016 | 0.0002 | 0.0008 | 0.0005 | 0.0001 | 0.0001 | 0.0013 | 0.002 | 0.0005 | 0.0002 | 0.0004 | 0.0001 | 0.0007 | 0.0013 | 0.0003 | 0.0002 | 0 | 0.0001 |
Nucleus | 0.0013 | 0.0006 | 0.0011 | 0.0007 | 0.0003 | 0.0007 | 0.0011 | 0.0011 | 0.001 | 0.0015 | 0.001 | 0.0006 | 0.0089 | 0.0009 | 0.0007 | 0.0007 | 0.0002 | 0.0013 |
Peroxisomes | 0.0234 | 0.0067 | 0.0195 | 0.0137 | 0.0437 | 0.0028 | 0.0106 | 0.0073 | 0.0179 | 0.0221 | 0.0361 | 0.0051 | 0.0056 | 0.0082 | 0.0088 | 0.0954 | 0.0023 | 0.0039 |
Punctate Nuclear | 0.003 | 0.0027 | 0.0051 | 0.0005 | 0.0012 | 0.0008 | 0.0036 | 0.0026 | 0.0034 | 0.0016 | 0.0067 | 0.0015 | 0.0056 | 0.001 | 0.001 | 0.0011 | 0.0003 | 0.0015 |
Vacuole | 0.0181 | 0.0098 | 0.0133 | 0.0281 | 0.0062 | 0.0136 | 0.0147 | 0.0116 | 0.0166 | 0.0209 | 0.0126 | 0.0107 | 0.0164 | 0.0145 | 0.0155 | 0.026 | 0.0102 | 0.0132 |
Vacuole Periphery | 0.0029 | 0.0014 | 0.0027 | 0.0041 | 0.0016 | 0.0035 | 0.0026 | 0.002 | 0.0028 | 0.0056 | 0.0029 | 0.0044 | 0.0019 | 0.0021 | 0.0025 | 0.0051 | 0.0034 | 0.0025 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.5817 | 5.7775 | 7.4051 | 8.8944 | 5.7133 | 5.8722 | 8.0986 | 10.036 | 7.6709 | 10.4018 |
Translational Efficiency | 1.0782 | 0.9036 | 0.7443 | 0.6128 | 0.9221 | 1.1961 | 0.8334 | 0.7023 | 0.8635 | 0.7302 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
2327 | 1447 | 1612 | 1717 | 1727 | 1476 | 2014 | 89 | 4054 | 2923 | 3626 | 1806 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 676.66 | 792.74 | 1034.77 | 1006.68 | 794.35 | 797.46 | 995.55 | 964.38 | 726.80 | 795.12 | 1012.99 | 1004.60 |
Standard Deviation | 82.71 | 119.40 | 136.64 | 134.42 | 94.02 | 100.74 | 114.98 | 142.35 | 105.26 | 110.40 | 126.58 | 135.14 |
Intensity Change Log 2 | — | 0.228417 | 0.612807 | 0.573102 | — | 0.005637 | 0.325719 | 0.279827 | — | 0.112401 | 0.464900 | 0.422165 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001292 | 0.000252 | 0.000309 | 0.000795 | 0.000176 | 0.000111 | 0.000131 | 0.000214 | 0.000816 | 0.000181 | 0.000210 | 0.000766 |
Bud Neck | 0.046585 | 0.008261 | 0.019844 | 0.011367 | 0.017262 | 0.009884 | 0.014927 | 0.020564 | 0.034094 | 0.009081 | 0.017113 | 0.011820 |
Bud Site | 0.031296 | 0.014958 | 0.030246 | 0.051688 | 0.011958 | 0.008556 | 0.017460 | 0.028510 | 0.023058 | 0.011725 | 0.023144 | 0.050546 |
Cell Periphery | 0.000234 | 0.000051 | 0.000138 | 0.000046 | 0.000058 | 0.000041 | 0.000037 | 0.000142 | 0.000159 | 0.000046 | 0.000082 | 0.000051 |
Cytoplasm | 0.065639 | 0.044668 | 0.018850 | 0.279881* | 0.033235 | 0.028092 | 0.132270* | 0.175587* | 0.051835 | 0.036298 | 0.081847 | 0.274741* |
Cytoplasmic Foci | 0.354004* | 0.287328* | 0.270337* | 0.203263* | 0.215911* | 0.221293* | 0.159942 | 0.141599 | 0.295177* | 0.253983* | 0.209020* | 0.200224* |
Eisosomes | 0.000169 | 0.000029 | 0.000038 | 0.000014 | 0.000033 | 0.000023 | 0.000011 | 0.000049 | 0.000111 | 0.000026 | 0.000023 | 0.000016 |
Endoplasmic Reticulum | 0.000968 | 0.000360 | 0.000396 | 0.000346 | 0.001203 | 0.000459 | 0.000874 | 0.000891 | 0.001068 | 0.000410 | 0.000662 | 0.000373 |
Endosome | 0.292281* | 0.495560* | 0.275441* | 0.141464 | 0.576537* | 0.542600* | 0.324582* | 0.181437 | 0.413374* | 0.519313* | 0.302736* | 0.143434 |
Golgi | 0.061761 | 0.035258 | 0.018668 | 0.007478 | 0.025738 | 0.035951 | 0.003664 | 0.001947 | 0.046415 | 0.035608 | 0.010334 | 0.007206 |
Lipid Particles | 0.024403 | 0.015213 | 0.027302 | 0.004527 | 0.017810 | 0.016149 | 0.004656 | 0.004096 | 0.021594 | 0.015686 | 0.014723 | 0.004506 |
Mitochondria | 0.020419 | 0.004784 | 0.002355 | 0.001688 | 0.004094 | 0.008218 | 0.000982 | 0.000581 | 0.013465 | 0.006518 | 0.001592 | 0.001634 |
Mitotic Spindle | 0.003722 | 0.016531 | 0.170791* | 0.075164 | 0.010211 | 0.021571 | 0.037581 | 0.014714 | 0.006486 | 0.019076 | 0.096802 | 0.072185 |
None | 0.001946 | 0.000628 | 0.000141 | 0.000281 | 0.000356 | 0.000710 | 0.000315 | 0.000262 | 0.001269 | 0.000669 | 0.000238 | 0.000280 |
Nuclear Periphery | 0.000081 | 0.000137 | 0.002171 | 0.000261 | 0.000210 | 0.000276 | 0.001124 | 0.000385 | 0.000136 | 0.000207 | 0.001589 | 0.000267 |
Nuclear Periphery Foci | 0.002177 | 0.004310 | 0.049312 | 0.002112 | 0.007352 | 0.004092 | 0.005115 | 0.002319 | 0.004382 | 0.004200 | 0.024763 | 0.002122 |
Nucleolus | 0.001111 | 0.001316 | 0.005474 | 0.000450 | 0.001422 | 0.001796 | 0.001892 | 0.002400 | 0.001243 | 0.001558 | 0.003485 | 0.000546 |
Nucleus | 0.003501 | 0.001223 | 0.002248 | 0.004321 | 0.002179 | 0.002355 | 0.007492 | 0.010363 | 0.002938 | 0.001794 | 0.005161 | 0.004618 |
Peroxisomes | 0.064264 | 0.030801 | 0.039389 | 0.026964 | 0.026500 | 0.028326 | 0.009134 | 0.012227 | 0.048176 | 0.029551 | 0.022584 | 0.026238 |
Vacuole | 0.019264 | 0.033371 | 0.059774 | 0.184866 | 0.043545 | 0.061098 | 0.271083* | 0.396208* | 0.029608 | 0.047372 | 0.177142 | 0.195281 |
Vacuole Periphery | 0.004884 | 0.004962 | 0.006777 | 0.003022 | 0.004209 | 0.008397 | 0.006730 | 0.005507 | 0.004596 | 0.006697 | 0.006751 | 0.003145 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.95 | 2.75 | 1.82 | -4.64 | -4.02 | 1.26 | 0.77 | -0.23 | -1.38 | -0.95 | 3.12 | 2.93 | 0.93 | -6.08 | -5.65 |
Bud Neck | 14.40 | 9.33 | 13.75 | -2.61 | 8.49 | 3.62 | 0.72 | -0.53 | -1.72 | -0.77 | 14.06 | 8.95 | 13.03 | -2.35 | 7.28 |
Bud Site | 6.95 | -0.10 | -3.41 | -10.23 | -3.41 | 2.31 | -3.36 | -1.97 | -2.39 | -1.27 | 7.78 | -0.48 | -7.39 | -13.07 | -7.25 |
Cell Periphery | 8.16 | 3.45 | 8.66 | 1.38 | 7.13 | 1.77 | 2.64 | -1.42 | -1.89 | -2.01 | 8.26 | 5.06 | 8.56 | -0.17 | 5.11 |
Cytoplasm | 4.51 | 11.55 | -32.11 | -34.43 | -39.28 | 1.42 | -20.09 | -6.59 | -6.78 | -2.12 | 5.16 | -10.81 | -35.56 | -37.73 | -28.50 |
Cytoplasmic Foci | 8.72 | 11.74 | 26.82 | 17.41 | 13.52 | -0.81 | 10.20 | 5.71 | 6.04 | 1.79 | 7.62 | 18.09 | 22.93 | 15.99 | 7.20 |
Eisosomes | 8.07 | 7.61 | 9.11 | 6.84 | 9.35 | 3.40 | 9.23 | -1.36 | -2.73 | -4.50 | 8.44 | 8.83 | 9.70 | 7.53 | 6.16 |
Endoplasmic Reticulum | 2.80 | 2.44 | 3.00 | 0.61 | 1.92 | 2.75 | 0.91 | 1.44 | -1.80 | 0.37 | 3.87 | 1.78 | 4.20 | 1.05 | 2.32 |
Endosome | -20.92 | 3.67 | 27.41* | 46.37* | 22.82* | 3.33 | 29.86 | 18.86 | 17.19 | 6.57 | -13.91 | 20.20 | 48.99* | 62.56* | 30.87* |
Golgi | 9.68 | 18.23 | 25.03 | 17.56 | 11.24 | -4.38 | 16.85 | 17.25 | 17.55 | 3.01 | 5.83 | 24.63 | 27.73 | 22.77 | 7.11 |
Lipid Particles | 4.24 | -0.63 | 11.99 | 7.33 | 10.74 | 0.62 | 8.06 | 8.12 | 6.83 | 1.67 | 3.56 | 5.13 | 14.18 | 9.59 | 9.90 |
Mitochondria | 9.82 | 12.27 | 13.20 | 5.10 | 2.70 | -3.19 | 3.94 | 4.52 | 7.50 | 2.61 | 6.40 | 13.03 | 13.32 | 8.29 | 1.63 |
Mitotic Spindle | -6.63 | -25.52* | -15.06 | -11.18 | 12.71 | -4.24 | -9.59 | -0.47 | 0.77 | 2.79 | -7.71 | -25.95 | -14.32 | -10.74 | 5.72 |
None | 2.65 | 5.20 | 4.65 | 0.85 | -2.21 | -1.17 | 0.75 | 1.49 | 2.02 | 1.29 | 2.07 | 5.09 | 4.63 | 1.66 | -0.74 |
Nuclear Periphery | -2.50 | -15.49 | -12.94 | -9.57 | 12.31 | -0.97 | -6.20 | -2.71 | -1.36 | 5.11 | -2.01 | -13.62 | -10.33 | -6.37 | 10.46 |
Nuclear Periphery Foci | -2.77 | -15.77 | -0.48 | 2.43 | 15.61 | 2.25 | 1.11 | 4.25 | 2.82 | 4.58 | -0.24 | -13.31 | 2.83 | 3.03 | 15.19 |
Nucleolus | -0.90 | -6.55 | 2.35 | 1.90 | 7.40 | -1.07 | -1.66 | -0.34 | 0.21 | 0.33 | -1.52 | -6.71 | 2.74 | 3.51 | 9.26 |
Nucleus | 3.03 | 0.57 | -5.80 | -11.97 | -7.77 | -0.43 | -8.22 | -3.81 | -3.63 | 0.10 | 1.88 | -6.14 | -8.25 | -10.95 | -1.89 |
Peroxisomes | 10.57 | 8.37 | 16.10 | 5.28 | 7.79 | -0.77 | 11.51 | 3.96 | 4.21 | -1.14 | 8.92 | 14.26 | 14.85 | 6.17 | 1.88 |
Vacuole | -5.92 | -15.13 | -38.76 | -34.84 | -28.40 | -3.84 | -38.38 | -12.54 | -11.92 | -4.60 | -7.05 | -38.65 | -38.43 | -33.96 | -10.02 |
Vacuole Periphery | -0.09 | -2.47 | 5.29 | 5.72 | 7.87 | -5.59 | -3.62 | 0.09 | 2.46 | 1.18 | -4.21 | -3.97 | 5.83 | 9.54 | 10.36 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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