Standard name
Human Ortholog
Description Sorting nexin; involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.1 0.09 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0.09 0.05 0.07 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.17 0.21 0.2 0.28 0.19 0.24 0.22 0.24 0.27 0.22 0.3 0.22 0.25 0.18 0.13 0.15 0.18 0.06 0.07 0.07 0 0.13 0.1
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.61 0.59 0.43 0.46 0.56 0.56 0.46 0.42 0.38 0.27 0.28 0.55 0.56 0.71 0.54 0.63 0.62 0.63 0.66 0.59 0.68 0.56 0.64
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0 0.06 0.18 0.09 0.43 0.26 0 0 0 0 0 0 0 0 0.09 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.3 0.25 0.38 0.32 0.26 0.24 0.31 0.22 0.29 0.15 0.23 0.28 0.34 0.36 0.41 0.37 0.37 0.19 0.13 0.12 0.13 0.13 0.12
Vac/Vac Membrane 0.05 0.2 0.11 0.08 0.1 0.08 0.06 0.1 0.08 0.07 0.06 0.18 0.06 0.07 0.16 0.14 0.17 0.05 0.06 0.05 0.06 0.07 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 4 8 10 7 12 13 5 8 1 9 1 6 3 11 3 13 1 2 2 0 1 3
Bud 5 2 4 3 6 8 12 16 16 14 13 1 3 2 1 1 5 0 2 0 0 1 1
Bud Neck 1 0 0 0 0 2 3 0 1 0 1 0 0 1 0 0 0 2 0 0 1 2 1
Bud Site 0 0 1 0 0 0 1 0 3 5 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 1 0 1 0 0 0 2 1 0 0 0 0 0 0 0
Cytoplasm 30 33 16 32 32 57 66 44 81 46 86 26 55 49 51 55 63 11 11 9 4 34 31
Endoplasmic Reticulum 2 0 0 4 3 6 8 1 3 4 8 3 1 3 11 10 15 0 0 1 0 2 1
Endosome 105 93 35 52 96 133 140 77 114 55 81 65 124 189 204 226 220 121 113 71 79 145 209
Golgi 0 0 2 0 0 1 0 0 0 0 0 1 0 0 7 4 6 3 0 0 0 0 1
Mitochondria 12 1 0 0 1 9 17 33 28 89 76 0 0 0 7 6 9 3 2 11 4 8 9
Nucleus 0 1 0 0 1 1 3 1 5 1 1 0 0 1 1 0 0 0 0 0 0 2 0
Nuclear Periphery 3 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 1 0 0 0 0 1 2
Nucleolus 10 8 1 3 4 3 6 2 2 9 6 3 6 2 17 15 21 0 0 0 1 1 1
Peroxisomes 4 0 0 1 0 2 5 1 4 3 5 0 2 9 5 6 1 0 0 0 0 0 0
SpindlePole 52 40 31 36 45 57 94 40 87 31 67 33 75 95 154 135 129 35 21 14 15 32 39
Vac/Vac Membrane 8 32 9 9 17 19 18 18 23 15 18 21 13 19 62 52 59 9 10 6 6 19 17
Unique Cell Count 173 158 82 114 170 237 305 182 304 206 287 119 222 265 378 361 352 192 171 121 117 260 326
Labelled Cell Count 236 214 107 150 212 310 386 239 376 273 373 155 285 373 534 514 542 192 171 121 117 260 326


Cytoplasm, Endosome, Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 7.5 6.8 6.8 7.5 6.7 6.8 5.7 6.0 5.2 5.6 7.2 7.4 7.4 10.3 9.8 9.3 6.4 6.8 7.0
Std Deviation (1e-4) 0.7 0.8 1.1 1.3 1.3 1.0 1.2 1.0 1.0 0.9 0.9 1.3 1.0 1.1 2.3 1.8 1.9 1.3 1.3 1.3
Intensity Change (Log2) -0.01 0.13 -0.03 -0.01 -0.26 -0.2 -0.39 -0.29 0.08 0.11 0.12 0.58 0.52 0.44 -0.09 0.0 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -0.2 0 -1.5 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1.1 0 0.6 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.4 -0.1 0.8 0.4 0.8 1.3 0.5 1.9 0.4 1.0 -0.2 -1.4 -1.0 -0.3
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.4 2.1 2.1 0.5 -0.1 -0.9 -2.6 -2.5 1.7 2.0 4.7 1.9 3.3 3.3
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 4.1 2.9 7.2 5.2 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.9 -1.8 -2.4 -1.2 -2.7 -1.6 -4.2 -2.6 -1.5 -0.7 -0.3 0.5 -0.1 -0.2
Vacuole -0.7 -0.2 -0.8 -1.6 -0.3 -1.0 -1.0 -1.4 1.3 -1.5 -1.1 1.2 0.8 1.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0638 0.7379 0.4164 0.1558 0.4066 0.8236 1.2656 2.1577 1.7655 1.1998 1.6995 1.7233 0.1915 1.1156 0.7899 0.7547 0.2516 0.5947
Actin 0.0336 0.0003 0.0271 0.0051 0.018 0.0007 0.0253 0.0002 0.0064 0.0018 0.0045 0.0002 0.0147 0.0001 0.0141 0.0137 0 0.0063
Bud 0.0008 0.0001 0.0019 0.0002 0.0003 0.0003 0.0005 0.0004 0.0006 0.0003 0.0008 0.0001 0.0007 0.0003 0.0004 0.0011 0 0.0001
Bud Neck 0.0022 0.0002 0.001 0.0033 0.0005 0.001 0.0055 0.0007 0.001 0.0003 0.0008 0.0004 0.0026 0.0003 0.001 0.0042 0.0001 0.0159
Bud Periphery 0.0011 0.0001 0.0009 0.0003 0.0004 0.0003 0.0012 0.0008 0.0006 0.0002 0.0004 0.0001 0.0007 0.0005 0.0005 0.0008 0 0.0001
Bud Site 0.0057 0.001 0.0031 0.0017 0.001 0.0003 0.0027 0.0024 0.0015 0.0003 0.0015 0.0001 0.007 0.0014 0.0029 0.1317 0.0001 0.0002
Cell Periphery 0.0004 0.0002 0.0002 0.0003 0.0001 0.0001 0.0003 0.0002 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.0002 0.0009 0 0
Cytoplasm 0.0616 0.1665 0.1491 0.1161 0.1497 0.2538 0.1442 0.1746 0.1415 0.1413 0.2551 0.1998 0.1815 0.2499 0.1923 0.1279 0.2277 0.31
Cytoplasmic Foci 0.2095 0.3188 0.2334 0.1729 0.2277 0.1867 0.1951 0.2953 0.2544 0.2362 0.2202 0.2745 0.2076 0.2392 0.2337 0.1708 0.1698 0.2124
Eisosomes 0.0006 0 0.0002 0.0002 0.0001 0 0.0003 0.0001 0.0001 0 0.0001 0 0.0003 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0077 0.0049 0.0057 0.0048 0.002 0.0048 0.0091 0.0025 0.0035 0.0037 0.0027 0.0027 0.0059 0.0082 0.0048 0.0012 0.0007 0.0051
Endosome 0.4904 0.4023 0.4157 0.518 0.3483 0.4347 0.4368 0.4068 0.4325 0.447 0.2924 0.4187 0.3674 0.3818 0.4316 0.2958 0.5346 0.3596
Golgi 0.0241 0.0124 0.0322 0.0251 0.0375 0.0164 0.0189 0.0115 0.0176 0.0133 0.028 0.0133 0.0123 0.0101 0.0211 0.0384 0.0109 0.0107
Lipid Particles 0.0523 0.0223 0.0292 0.0108 0.043 0.0201 0.0494 0.0187 0.02 0.0057 0.0173 0.013 0.0371 0.0143 0.0264 0.0789 0.0379 0.0133
Mitochondria 0.0093 0.0123 0.005 0.0345 0.0713 0.0242 0.0068 0.0008 0.0072 0.0057 0.0036 0.0026 0.0077 0.0069 0.0083 0.0035 0.0004 0.0008
None 0.0467 0.0361 0.0505 0.0579 0.0464 0.0339 0.0658 0.0574 0.0694 0.0862 0.1117 0.0512 0.1112 0.0562 0.0324 0.002 0.001 0.04
Nuclear Periphery 0.0035 0.001 0.0023 0.0012 0.0004 0.0012 0.0041 0.001 0.0014 0.006 0.0013 0.0009 0.004 0.0027 0.0014 0.0006 0.0004 0.003
Nucleolus 0.0016 0.0002 0.0008 0.0005 0.0001 0.0001 0.0013 0.002 0.0005 0.0002 0.0004 0.0001 0.0007 0.0013 0.0003 0.0002 0 0.0001
Nucleus 0.0013 0.0006 0.0011 0.0007 0.0003 0.0007 0.0011 0.0011 0.001 0.0015 0.001 0.0006 0.0089 0.0009 0.0007 0.0007 0.0002 0.0013
Peroxisomes 0.0234 0.0067 0.0195 0.0137 0.0437 0.0028 0.0106 0.0073 0.0179 0.0221 0.0361 0.0051 0.0056 0.0082 0.0088 0.0954 0.0023 0.0039
Punctate Nuclear 0.003 0.0027 0.0051 0.0005 0.0012 0.0008 0.0036 0.0026 0.0034 0.0016 0.0067 0.0015 0.0056 0.001 0.001 0.0011 0.0003 0.0015
Vacuole 0.0181 0.0098 0.0133 0.0281 0.0062 0.0136 0.0147 0.0116 0.0166 0.0209 0.0126 0.0107 0.0164 0.0145 0.0155 0.026 0.0102 0.0132
Vacuole Periphery 0.0029 0.0014 0.0027 0.0041 0.0016 0.0035 0.0026 0.002 0.0028 0.0056 0.0029 0.0044 0.0019 0.0021 0.0025 0.0051 0.0034 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.5817 5.7775 7.4051 8.8944 5.7133 5.8722 8.0986 10.036 7.6709 10.4018
Translational Efficiency 1.0782 0.9036 0.7443 0.6128 0.9221 1.1961 0.8334 0.7023 0.8635 0.7302

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2327 1447 1612 1717 1727 1476 2014 89 4054 2923 3626 1806

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 676.66 792.74 1034.77 1006.68 794.35 797.46 995.55 964.38 726.80 795.12 1012.99 1004.60
Standard Deviation 82.71 119.40 136.64 134.42 94.02 100.74 114.98 142.35 105.26 110.40 126.58 135.14
Intensity Change Log 2 0.228417 0.612807 0.573102 0.005637 0.325719 0.279827 0.112401 0.464900 0.422165

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001292 0.000252 0.000309 0.000795 0.000176 0.000111 0.000131 0.000214 0.000816 0.000181 0.000210 0.000766
Bud Neck 0.046585 0.008261 0.019844 0.011367 0.017262 0.009884 0.014927 0.020564 0.034094 0.009081 0.017113 0.011820
Bud Site 0.031296 0.014958 0.030246 0.051688 0.011958 0.008556 0.017460 0.028510 0.023058 0.011725 0.023144 0.050546
Cell Periphery 0.000234 0.000051 0.000138 0.000046 0.000058 0.000041 0.000037 0.000142 0.000159 0.000046 0.000082 0.000051
Cytoplasm 0.065639 0.044668 0.018850 0.279881* 0.033235 0.028092 0.132270* 0.175587* 0.051835 0.036298 0.081847 0.274741*
Cytoplasmic Foci 0.354004* 0.287328* 0.270337* 0.203263* 0.215911* 0.221293* 0.159942 0.141599 0.295177* 0.253983* 0.209020* 0.200224*
Eisosomes 0.000169 0.000029 0.000038 0.000014 0.000033 0.000023 0.000011 0.000049 0.000111 0.000026 0.000023 0.000016
Endoplasmic Reticulum 0.000968 0.000360 0.000396 0.000346 0.001203 0.000459 0.000874 0.000891 0.001068 0.000410 0.000662 0.000373
Endosome 0.292281* 0.495560* 0.275441* 0.141464 0.576537* 0.542600* 0.324582* 0.181437 0.413374* 0.519313* 0.302736* 0.143434
Golgi 0.061761 0.035258 0.018668 0.007478 0.025738 0.035951 0.003664 0.001947 0.046415 0.035608 0.010334 0.007206
Lipid Particles 0.024403 0.015213 0.027302 0.004527 0.017810 0.016149 0.004656 0.004096 0.021594 0.015686 0.014723 0.004506
Mitochondria 0.020419 0.004784 0.002355 0.001688 0.004094 0.008218 0.000982 0.000581 0.013465 0.006518 0.001592 0.001634
Mitotic Spindle 0.003722 0.016531 0.170791* 0.075164 0.010211 0.021571 0.037581 0.014714 0.006486 0.019076 0.096802 0.072185
None 0.001946 0.000628 0.000141 0.000281 0.000356 0.000710 0.000315 0.000262 0.001269 0.000669 0.000238 0.000280
Nuclear Periphery 0.000081 0.000137 0.002171 0.000261 0.000210 0.000276 0.001124 0.000385 0.000136 0.000207 0.001589 0.000267
Nuclear Periphery Foci 0.002177 0.004310 0.049312 0.002112 0.007352 0.004092 0.005115 0.002319 0.004382 0.004200 0.024763 0.002122
Nucleolus 0.001111 0.001316 0.005474 0.000450 0.001422 0.001796 0.001892 0.002400 0.001243 0.001558 0.003485 0.000546
Nucleus 0.003501 0.001223 0.002248 0.004321 0.002179 0.002355 0.007492 0.010363 0.002938 0.001794 0.005161 0.004618
Peroxisomes 0.064264 0.030801 0.039389 0.026964 0.026500 0.028326 0.009134 0.012227 0.048176 0.029551 0.022584 0.026238
Vacuole 0.019264 0.033371 0.059774 0.184866 0.043545 0.061098 0.271083* 0.396208* 0.029608 0.047372 0.177142 0.195281
Vacuole Periphery 0.004884 0.004962 0.006777 0.003022 0.004209 0.008397 0.006730 0.005507 0.004596 0.006697 0.006751 0.003145

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.95 2.75 1.82 -4.64 -4.02 1.26 0.77 -0.23 -1.38 -0.95 3.12 2.93 0.93 -6.08 -5.65
Bud Neck 14.40 9.33 13.75 -2.61 8.49 3.62 0.72 -0.53 -1.72 -0.77 14.06 8.95 13.03 -2.35 7.28
Bud Site 6.95 -0.10 -3.41 -10.23 -3.41 2.31 -3.36 -1.97 -2.39 -1.27 7.78 -0.48 -7.39 -13.07 -7.25
Cell Periphery 8.16 3.45 8.66 1.38 7.13 1.77 2.64 -1.42 -1.89 -2.01 8.26 5.06 8.56 -0.17 5.11
Cytoplasm 4.51 11.55 -32.11 -34.43 -39.28 1.42 -20.09 -6.59 -6.78 -2.12 5.16 -10.81 -35.56 -37.73 -28.50
Cytoplasmic Foci 8.72 11.74 26.82 17.41 13.52 -0.81 10.20 5.71 6.04 1.79 7.62 18.09 22.93 15.99 7.20
Eisosomes 8.07 7.61 9.11 6.84 9.35 3.40 9.23 -1.36 -2.73 -4.50 8.44 8.83 9.70 7.53 6.16
Endoplasmic Reticulum 2.80 2.44 3.00 0.61 1.92 2.75 0.91 1.44 -1.80 0.37 3.87 1.78 4.20 1.05 2.32
Endosome -20.92 3.67 27.41* 46.37* 22.82* 3.33 29.86 18.86 17.19 6.57 -13.91 20.20 48.99* 62.56* 30.87*
Golgi 9.68 18.23 25.03 17.56 11.24 -4.38 16.85 17.25 17.55 3.01 5.83 24.63 27.73 22.77 7.11
Lipid Particles 4.24 -0.63 11.99 7.33 10.74 0.62 8.06 8.12 6.83 1.67 3.56 5.13 14.18 9.59 9.90
Mitochondria 9.82 12.27 13.20 5.10 2.70 -3.19 3.94 4.52 7.50 2.61 6.40 13.03 13.32 8.29 1.63
Mitotic Spindle -6.63 -25.52* -15.06 -11.18 12.71 -4.24 -9.59 -0.47 0.77 2.79 -7.71 -25.95 -14.32 -10.74 5.72
None 2.65 5.20 4.65 0.85 -2.21 -1.17 0.75 1.49 2.02 1.29 2.07 5.09 4.63 1.66 -0.74
Nuclear Periphery -2.50 -15.49 -12.94 -9.57 12.31 -0.97 -6.20 -2.71 -1.36 5.11 -2.01 -13.62 -10.33 -6.37 10.46
Nuclear Periphery Foci -2.77 -15.77 -0.48 2.43 15.61 2.25 1.11 4.25 2.82 4.58 -0.24 -13.31 2.83 3.03 15.19
Nucleolus -0.90 -6.55 2.35 1.90 7.40 -1.07 -1.66 -0.34 0.21 0.33 -1.52 -6.71 2.74 3.51 9.26
Nucleus 3.03 0.57 -5.80 -11.97 -7.77 -0.43 -8.22 -3.81 -3.63 0.10 1.88 -6.14 -8.25 -10.95 -1.89
Peroxisomes 10.57 8.37 16.10 5.28 7.79 -0.77 11.51 3.96 4.21 -1.14 8.92 14.26 14.85 6.17 1.88
Vacuole -5.92 -15.13 -38.76 -34.84 -28.40 -3.84 -38.38 -12.54 -11.92 -4.60 -7.05 -38.65 -38.43 -33.96 -10.02
Vacuole Periphery -0.09 -2.47 5.29 5.72 7.87 -5.59 -3.62 0.09 2.46 1.18 -4.21 -3.97 5.83 9.54 10.36
Standard name
Human Ortholog
Description Sorting nexin; involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p
Localization
Cell Percentages endosome (69%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Snx41

Snx41


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Snx41-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available