Standard name
Human Ortholog
Description Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis; shares similarity with mammalian PIG-P
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.1 0.06 0 0 0 0 0 0 0
Cytoplasm 0.61 0.6 0.74 0.61 0.7 0.61 – 0.51 0.45 0.48 0.47 0.37 0.82 0.84 0.86 0.69 0.65 0.83 0.27 0.18 0.13 0.26 0.19 0.23
Endoplasmic Reticulum 0.27 0.36 0 0.11 0 0 – 0 0 0 0 0.05 0 0.1 0 0 0.09 0 0.5 0.57 0.54 0.42 0.52 0.43
Endosome 0 0 0 0 0.07 0 – 0 0 0 0 0 0.05 0.05 0 0.06 0.05 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.13 0 0.26 0.44 0.18 0.49 – 0.53 0.66 0.64 0.65 0.69 0 0 0 0.05 0.12 0 0.1 0.11 0.15 0.14 0.11 0.07
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.08 0 0 0.11 0.08 – 0.07 0.09 0.07 0 0 0.12 0 0.1 0.21 0.18 0.13 0.06 0.07 0 0.06 0 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 1 0 0 – 0 0 0 0 0 0 1 0 0 4 0 2 9 8 4 9 10
Bud 1 0 7 6 3 7 – 14 14 14 23 24 0 1 1 1 1 0 0 1 2 1 4 8
Bud Neck 0 0 0 0 0 0 – 0 0 0 1 0 0 1 0 0 0 0 1 4 2 0 2 3
Bud Site 0 0 0 0 0 1 – 1 0 0 3 2 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 3 2 5 3 4 – 2 8 7 11 17 1 4 7 13 7 1 0 2 1 0 2 5
Cytoplasm 69 183 187 135 184 228 – 309 197 246 255 204 146 220 243 91 75 63 39 70 38 45 48 63
Endoplasmic Reticulum 31 110 11 25 5 12 – 6 13 10 6 25 5 25 1 4 10 2 73 228 152 71 134 117
Endosome 0 0 2 0 18 2 – 13 3 3 3 3 8 13 8 8 6 0 1 1 6 2 3 6
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 1 0 1 1 1 1 3 1
Mitochondria 15 2 65 97 47 184 – 320 291 326 356 378 3 3 2 7 14 2 15 43 42 24 28 18
Nucleus 1 2 4 2 0 3 – 8 4 10 7 4 0 1 2 1 0 0 0 0 0 1 0 0
Nuclear Periphery 0 2 2 0 1 4 – 14 5 3 3 6 0 0 1 0 0 0 0 4 3 2 0 1
Nucleolus 0 0 3 1 0 2 – 0 0 0 0 1 0 1 3 0 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 0 – 0 0 0 1 3 1 0 1 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 7 0 0 0 1 0 0 1 1 3 2 1 1
Vac/Vac Membrane 6 24 7 5 29 28 – 44 38 38 19 21 22 10 28 28 21 10 8 29 11 10 11 21
Unique Cell Count 114 307 254 220 261 372 607 440 511 547 546 177 262 281 131 116 76 147 402 284 171 261 273
Labelled Cell Count 126 328 290 277 290 475 731 573 657 688 695 186 280 298 155 139 78 147 402 284 171 261 273


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.4 4.4 4.8 5.3 4.1 – 4.1 3.7 3.7 3.7 3.6 6.3 6.4 6.7 7.5 7.2 7.2 6.0 6.7 7.1
Std Deviation (1e-4) 0.5 0.9 1.6 2.7 2.1 1.6 – 1.4 1.7 0.7 1.3 1.1 2.5 2.6 3.1 1.8 1.7 2.6 2.0 2.7 2.8
Intensity Change (Log2) – – – 0.14 0.27 -0.1 – -0.1 -0.24 -0.23 -0.23 -0.27 0.52 0.55 0.62 0.77 0.71 0.73 0.45 0.62 0.71


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 0 0 0 4.4 0 0
Cytoplasm -2.9 -0.8 -3.2 – -6.2 -7.4 -6.7 -7.2 -9.6 2.2 2.9 3.7 -0.9 -1.8 1.7
Endoplasmic Reticulum 0 -1.6 0 – 0 0 0 0 0 -0.8 2.3 -3.1 -0.6 1.7 0
Endosome 0 3.6 0 – 0 0 0 0 0 0 2.8 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 -2.1 0 – 0 0 0 0 0 -6.7 -8.2 -8.7 -4.8 -2.9 -4.4
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.3 3.7 2.6 – 2.5 3.0 2.6 0.5 0.8 3.9 0.7 3.4 6.0 5.2 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.9466 0.6962 0.6023 0.205 0.7311 0.4753 0.2672 0.361 0.5345 0.1529 0.2424 0.2345 1.9279 1.7299 1.7177 1.7388 2.3262 1.2717
Actin 0.0296 0.0002 0.0111 0.0037 0.0001 0.0216 0.0215 0.0011 0.0088 0.0027 0.0216 0.0033 0.0128 0.0003 0.0152 0.0011 0.0245 0.0005
Bud 0.0002 0 0.0003 0.0005 0.0001 0.0001 0.0121 0.0201 0.0076 0.0398 0.0014 0.0061 0.0002 0 0.0003 0.0002 0.0028 0
Bud Neck 0.0004 0.0001 0.0006 0.0186 0.0005 0.0009 0.0037 0.0005 0.0017 0.0005 0.0025 0.0021 0.0004 0.0001 0.0042 0.0002 0.0102 0.0009
Bud Periphery 0.0002 0 0.0002 0.0008 0.0001 0.0001 0.007 0.004 0.0028 0.0102 0.0019 0.0033 0.0003 0 0.0007 0.0007 0.0064 0.0001
Bud Site 0.0008 0.0003 0.0038 0.0028 0.0017 0.0003 0.0094 0.0267 0.0132 0.0027 0.0081 0.0006 0.001 0.0006 0.002 0.0003 0.0037 0.0001
Cell Periphery 0.0005 0.0007 0.0006 0.0007 0.0009 0.0003 0.0021 0.0021 0.0007 0.0006 0.0007 0.0006 0.0009 0.0006 0.0008 0.0006 0.0008 0.0003
Cytoplasm 0.2079 0.3744 0.3116 0.3035 0.3861 0.4564 0.2587 0.3619 0.2746 0.2644 0.3063 0.448 0.2495 0.3357 0.3126 0.3526 0.3641 0.4536
Cytoplasmic Foci 0.0485 0.007 0.0219 0.0113 0.0147 0.0095 0.0567 0.0117 0.0334 0.022 0.0288 0.0283 0.0115 0.0068 0.0122 0.0038 0.0203 0.0068
Eisosomes 0.0002 0.0001 0.0001 0.0002 0 0.0002 0.0006 0.0002 0.0002 0.0002 0.0003 0.0001 0.0004 0.0001 0.0005 0.0002 0.0005 0.0001
Endoplasmic Reticulum 0.1597 0.1905 0.2065 0.2579 0.1043 0.1787 0.1691 0.1514 0.1979 0.1974 0.1817 0.1499 0.1971 0.203 0.2174 0.2334 0.1401 0.2638
Endosome 0.0524 0.0115 0.0129 0.0266 0.1085 0.0088 0.0412 0.0133 0.0372 0.0463 0.0448 0.0226 0.0157 0.0117 0.0119 0.0044 0.0129 0.0132
Golgi 0.0262 0.0003 0.0061 0.022 0.0073 0.008 0.0108 0.0008 0.0086 0.0118 0.031 0.0031 0.0024 0.0005 0.0027 0.0002 0.0041 0.0003
Lipid Particles 0.0564 0.0038 0.0132 0.0049 0.0859 0.0314 0.0245 0.0065 0.0161 0.0077 0.0079 0.0043 0.0128 0.0041 0.0048 0.0013 0.0134 0.0024
Mitochondria 0.0101 0.0001 0.004 0.0036 0.0028 0.0099 0.0104 0.0116 0.0035 0.0027 0.0318 0.0157 0.0014 0.0003 0.0019 0.0005 0.0035 0.0012
None 0.2559 0.3951 0.3804 0.3189 0.2452 0.2612 0.3106 0.3386 0.32 0.3453 0.286 0.2472 0.4601 0.417 0.366 0.3804 0.2956 0.2289
Nuclear Periphery 0.054 0.0082 0.0083 0.0109 0.0045 0.0034 0.0172 0.02 0.0284 0.0128 0.0185 0.028 0.0174 0.011 0.0154 0.012 0.0141 0.0151
Nucleolus 0.0005 0.0001 0.0003 0.0003 0.0022 0.0001 0.0074 0.0015 0.001 0.0007 0.0043 0.0008 0.0005 0.0001 0.0003 0.0002 0.0075 0.0001
Nucleus 0.033 0.0024 0.0017 0.0026 0.0027 0.0017 0.0063 0.0114 0.0095 0.0075 0.0046 0.0114 0.0056 0.0023 0.0082 0.0039 0.0308 0.0072
Peroxisomes 0.0381 0 0.0111 0.0025 0.002 0.0046 0.0088 0.0003 0.012 0.0035 0.0037 0.001 0.0013 0.0001 0.0125 0.0002 0.0129 0.0001
Punctate Nuclear 0.0189 0.0009 0.001 0.001 0.0009 0.0006 0.0046 0.0063 0.0136 0.0033 0.0037 0.0165 0.0033 0.0009 0.006 0.0007 0.0246 0.0008
Vacuole 0.0029 0.0039 0.0036 0.0058 0.027 0.002 0.0143 0.0091 0.0076 0.0151 0.0088 0.0058 0.0043 0.0043 0.0038 0.0028 0.0056 0.0038
Vacuole Periphery 0.0036 0.0002 0.0004 0.001 0.0025 0.0003 0.0031 0.0008 0.0015 0.0029 0.0019 0.0012 0.0011 0.0004 0.0007 0.0003 0.0019 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.217 9.3274 5.191 6.4447 10.1462 7.8212 5.8943 5.5696 6.2299 6.2407
Translational Efficiency 1.9725 0.8694 1.6287 1.3926 0.7985 1.1016 1.9462 1.3966 1.4749 1.5012

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1694 1066 799 145 599 7 1984 1219 2293 1073 2783 1364

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 713.50 775.06 938.29 938.31 595.02 795.42 904.86 876.00 682.55 775.19 914.46 882.62
Standard Deviation 81.80 99.29 114.64 112.30 80.31 107.06 104.99 110.78 96.63 99.36 108.90 112.60
Intensity Change Log 2 — 0.119395 0.395120 0.395151 — 0.418779 0.604756 0.557993 — 0.263270 0.494238 0.471485

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000138 0.001327 0.000257 0.000803 0.000258 0.000044 0.000238 0.001414 0.000169 0.001318 0.000243 0.001349
Bud Neck 0.005840 0.011252 0.002561 0.002661 0.009558 0.002500 0.003808 0.006992 0.006811 0.011195 0.003450 0.006531
Bud Site 0.001449 0.011120 0.006056 0.013633 0.005542 0.000827 0.004014 0.020267 0.002518 0.011053 0.004600 0.019562
Cell Periphery 0.000412 0.000666 0.000378 0.000382 0.001504 0.000496 0.000331 0.000843 0.000697 0.000665 0.000345 0.000794
Cytoplasm 0.349768* 0.256358* 0.308535* 0.206819* 0.397775* 0.398109* 0.342822* 0.310683* 0.362309* 0.257283* 0.332978* 0.299642*
Cytoplasmic Foci 0.170932 0.208472* 0.057918 0.075929 0.174475 0.229157* 0.024302 0.063185 0.171857 0.208607* 0.033953 0.064539
Eisosomes 0.000638 0.000818 0.000134 0.000242 0.000924 0.000453 0.000081 0.000366 0.000713 0.000816 0.000096 0.000352
Endoplasmic Reticulum 0.129874* 0.151958* 0.029050 0.076635 0.085772 0.050529 0.091182 0.172056* 0.118353* 0.151297* 0.073344 0.161913*
Endosome 0.012797 0.027641 0.011692 0.017708 0.007662 0.016118 0.006573 0.014533 0.011455 0.027566 0.008043 0.014871
Golgi 0.011091 0.019711 0.000200 0.000317 0.010551 0.003073 0.000463 0.001575 0.010950 0.019602 0.000387 0.001442
Lipid Particles 0.152205* 0.121021* 0.015383 0.031300 0.143770* 0.126294* 0.014360 0.033189 0.150002* 0.121056* 0.014654 0.032988
Mitochondria 0.007386 0.013265 0.000939 0.004829 0.026551 0.001367 0.000968 0.003140 0.012393 0.013187 0.000960 0.003320
Mitotic Spindle 0.000413 0.004324 0.000511 0.002730 0.000118 0.000014 0.001046 0.009059 0.000336 0.004296 0.000892 0.008386
None 0.002264 0.003749 0.005579 0.000892 0.005869 0.000327 0.001880 0.000596 0.003206 0.003727 0.002942 0.000628
Nuclear Periphery 0.008450 0.007556 0.032717 0.016399 0.005655 0.025908 0.071135 0.019550 0.007720 0.007675 0.060105 0.019215
Nuclear Periphery Foci 0.004732 0.008702 0.042748 0.099838 0.003223 0.023096 0.046266 0.044691 0.004338 0.008796 0.045256 0.050553
Nucleolus 0.000651 0.001094 0.002143 0.000417 0.001746 0.000664 0.000663 0.000433 0.000937 0.001091 0.001088 0.000431
Nucleus 0.095790 0.074164 0.383885* 0.136477* 0.089862 0.074935 0.315723* 0.129827 0.094241 0.074170 0.335292* 0.130533*
Peroxisomes 0.004800 0.010488 0.001011 0.002397 0.011057 0.002309 0.000767 0.002132 0.006435 0.010435 0.000837 0.002160
Vacuole 0.039025 0.062894 0.097834 0.306750* 0.015501 0.042341 0.072422 0.164314 0.032880 0.062760 0.079718 0.179456
Vacuole Periphery 0.001345 0.003421 0.000469 0.002841 0.002626 0.001437 0.000957 0.001157 0.001680 0.003408 0.000817 0.001336

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.98 -4.00 -2.44 1.40 -1.87 NaN NaN NaN NaN NaN -4.85 -3.42 -4.13 -0.05 -3.73
Bud Neck -3.77 4.36 2.26 5.22 -1.60 NaN NaN NaN NaN NaN -3.11 4.47 -0.23 3.05 -5.21
Bud Site -5.00 -2.97 -2.30 -0.44 -1.36 NaN NaN NaN NaN NaN -4.37 -2.53 -7.12 -2.66 -5.99
Cell Periphery -1.27 0.52 2.34 1.80 2.27 NaN NaN NaN NaN NaN 0.16 5.20 -0.33 -0.32 -4.42
Cytoplasm 10.68 4.64 7.70 2.45 4.70 NaN NaN NaN NaN NaN 12.64 5.35 8.36 -3.79 3.94
Cytoplasmic Foci -5.19 22.57 11.60 14.15 -1.12 NaN NaN NaN NaN NaN -5.36 38.13 26.12 22.52 -10.99
Eisosomes -1.08 11.79 8.51 3.76 -2.50 NaN NaN NaN NaN NaN -0.62 16.68 8.40 3.00 -8.65
Endoplasmic Reticulum -2.33 18.00 7.80 8.76 -1.64 NaN NaN NaN NaN NaN -3.74 9.77 -2.38 1.45 -9.95
Endosome -9.66 0.40 -0.70 6.60 -0.90 NaN NaN NaN NaN NaN -10.85 5.39 -2.42 8.47 -6.08
Golgi -5.45 16.13 16.07 13.73 -0.82 NaN NaN NaN NaN NaN -5.65 17.78 15.52 12.81 -2.67
Lipid Particles 5.03 31.86* 24.12 17.13 -1.44 NaN NaN NaN NaN NaN 4.98 37.12 31.30 18.84 -10.45
Mitochondria -3.30 6.49 1.91 3.85 -0.77 NaN NaN NaN NaN NaN -0.40 11.16 7.82 6.29 -2.42
Mitotic Spindle -3.05 -0.30 -1.96 0.81 -1.88 NaN NaN NaN NaN NaN -3.14 -2.35 -4.78 -1.86 -4.14
None -1.18 -2.19 2.01 1.91 2.82 NaN NaN NaN NaN NaN -0.39 0.62 6.74 2.48 4.35
Nuclear Periphery 0.84 -7.07 -0.76 -1.03 4.72 NaN NaN NaN NaN NaN 0.07 -19.95 -5.11 -4.72 13.27
Nuclear Periphery Foci -4.53 -11.00 -5.53 -5.13 -1.81 NaN NaN NaN NaN NaN -5.13 -21.21 -17.94 -14.98 0.11
Nucleolus -2.43 -6.18 1.28 3.31 6.90 NaN NaN NaN NaN NaN -0.48 -0.72 2.73 3.52 6.30
Nucleus 5.28 -27.50 -2.31 -3.59 12.74 NaN NaN NaN NaN NaN 5.20 -44.91 -7.31 -10.30 31.31
Peroxisomes -6.85 10.86 1.43 4.60 -0.99 NaN NaN NaN NaN NaN -4.43 11.45 7.80 10.01 -3.44
Vacuole -6.76 -12.58 -14.54 -13.48 -11.42 NaN NaN NaN NaN NaN -8.83 -20.13 -28.70 -22.15 -18.40
Vacuole Periphery -5.89 10.32 -0.16 1.31 -0.80 NaN NaN NaN NaN NaN -4.88 3.11 1.73 4.75 -0.63
Standard name
Human Ortholog
Description Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis; shares similarity with mammalian PIG-P
Localization
Cell Percentages ER (67%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Gpi19

Gpi19


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gpi19-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available