Standard name | |
---|---|
Human Ortholog | |
Description | Nucleolar protein that forms a complex with Csm1p; and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 7 | 0 | 1 | 0 | 2 | 3 | 9 | 14 | 11 | 15 | 9 | 10 | 1 | 0 | 0 | 1 | 0 | 1 | 3 | 2 | 0 | 0 | 0 | 3 |
Bud Neck | 3 | 1 | 6 | 14 | 20 | 8 | 25 | 37 | 9 | 23 | 16 | 17 | 14 | 13 | 7 | 2 | 1 | 4 | 3 | 2 | 0 | 1 | 3 | 4 |
Bud Site | 3 | 0 | 0 | 5 | 4 | 6 | 13 | 15 | 15 | 22 | 13 | 16 | 0 | 0 | 0 | 0 | 0 | 4 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 8 | 6 | 5 | 7 | 21 | 17 | 24 | 26 | 21 | 21 | 13 | 9 | 9 | 15 | 27 | 11 | 4 | 6 | 1 | 1 | 3 | 0 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 |
Endosome | 4 | 14 | 3 | 5 | 14 | 12 | 15 | 1 | 4 | 0 | 0 | 1 | 11 | 20 | 27 | 28 | 14 | 25 | 54 | 30 | 19 | 7 | 18 | 18 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 |
Mitochondria | 169 | 23 | 14 | 14 | 6 | 46 | 84 | 58 | 87 | 104 | 107 | 90 | 6 | 1 | 1 | 17 | 5 | 19 | 12 | 4 | 7 | 0 | 1 | 1 |
Nucleus | 49 | 45 | 21 | 44 | 30 | 23 | 33 | 35 | 26 | 38 | 28 | 25 | 30 | 27 | 20 | 16 | 7 | 17 | 21 | 20 | 8 | 1 | 2 | 3 |
Nuclear Periphery | 2 | 2 | 1 | 1 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 12 | 5 | 2 | 0 | 0 | 0 |
Nucleolus | 58 | 39 | 9 | 27 | 22 | 37 | 83 | 54 | 68 | 82 | 63 | 55 | 69 | 79 | 55 | 32 | 23 | 38 | 88 | 53 | 24 | 21 | 37 | 33 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 3 | 0 | 0 | 1 | 0 | 2 | 0 |
SpindlePole | 50 | 22 | 31 | 73 | 121 | 82 | 145 | 157 | 131 | 141 | 79 | 94 | 111 | 135 | 119 | 56 | 23 | 39 | 56 | 37 | 33 | 33 | 72 | 102 |
Vac/Vac Membrane | 11 | 11 | 0 | 2 | 6 | 4 | 2 | 3 | 0 | 6 | 3 | 4 | 1 | 0 | 2 | 6 | 3 | 7 | 21 | 9 | 11 | 0 | 6 | 6 |
Unique Cell Count | 253 | 127 | 65 | 143 | 183 | 159 | 277 | 270 | 232 | 271 | 208 | 197 | 179 | 186 | 170 | 111 | 50 | 97 | 291 | 173 | 117 | 72 | 154 | 184 |
Labelled Cell Count | 364 | 163 | 91 | 192 | 247 | 242 | 441 | 400 | 373 | 452 | 332 | 321 | 252 | 293 | 262 | 174 | 80 | 165 | 291 | 173 | 117 | 72 | 154 | 184 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.7 | 5.0 | 4.8 | 5.0 | 5.3 | 4.3 | 4.1 | 4.2 | 3.8 | 3.7 | 3.7 | 3.8 | 5.1 | 5.6 | 5.2 | 6.6 | 6.7 | 6.1 | 4.5 | 5.3 | 5.6 |
Std Deviation (1e-4) | 0.4 | 0.8 | 1.1 | 1.6 | 1.8 | 1.4 | 1.8 | 1.4 | 1.7 | 1.2 | 1.5 | 1.0 | 0.8 | 1.1 | 1.1 | 1.3 | 1.3 | 1.2 | 1.1 | 1.2 | 1.1 |
Intensity Change (Log2) | – | – | – | 0.04 | 0.13 | -0.15 | -0.25 | -0.2 | -0.34 | -0.39 | -0.39 | -0.35 | 0.08 | 0.21 | 0.12 | 0.45 | 0.47 | 0.34 | -0.09 | 0.13 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.1 | 0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0.9 | 0.7 | 0.3 | 0.5 | 0.3 | 0 | 0 | 0 | 0 | 0.1 | 1.6 | 0.5 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.5 | 2.3 | 3.5 | 3.5 | 3.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.3 | -4.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -4.6 | 0 | 0 | -1.0 | -1.7 | -0.3 |
Nucleus | -0.2 | -2.7 | -3.1 | -4.1 | -3.8 | -4.1 | -3.5 | -3.5 | -3.6 | -2.6 | -3.1 | -3.7 | -2.8 | -2.3 | -2.2 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.9 | -0.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.7 | 4.2 | 2.9 | 2.3 | 3.8 | 3.5 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0.4 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.0 | 3.6 | 3.2 | 0.4 | -0.2 | -0.9 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.3622 | 0.9282 | 1.2119 | 0.5133 | 0.3786 | 1.0723 | 0.513 | 0.3745 | 0.3838 | 0.6682 | -0.2979 | 0.233 | 0.5557 | -0.0117 | -0.7929 | -0.4165 | -0.7541 | -0.7891 |
Actin | 0.0029 | 0.0001 | 0.0023 | 0 | 0.0007 | 0.0006 | 0.0086 | 0.0001 | 0.0031 | 0.0019 | 0.0056 | 0.001 | 0.0164 | 0.0001 | 0.0009 | 0 | 0.0005 | 0.0014 |
Bud | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Bud Neck | 0.0017 | 0.0018 | 0.0023 | 0.0022 | 0.0307 | 0.0035 | 0.003 | 0.0048 | 0.0031 | 0.0033 | 0.0217 | 0.0021 | 0.0022 | 0.0022 | 0.0031 | 0.0151 | 0.0017 | 0.0029 |
Bud Periphery | 0.0007 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0003 | 0.0007 | 0.0003 | 0.0006 | 0.0007 | 0.0002 | 0.0004 | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0008 |
Bud Site | 0.0023 | 0.0013 | 0.0017 | 0.0003 | 0.0007 | 0.0004 | 0.0063 | 0.0046 | 0.0018 | 0.0008 | 0.0004 | 0.0004 | 0.0023 | 0.0014 | 0.0022 | 0.0017 | 0.0004 | 0.0009 |
Cell Periphery | 0.0005 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0003 | 0.0005 | 0.0008 | 0.0015 | 0.0001 | 0.0002 |
Cytoplasm | 0.0109 | 0.001 | 0.002 | 0.0001 | 0.0002 | 0.0021 | 0.0015 | 0.0018 | 0.001 | 0.0005 | 0.0002 | 0.0012 | 0.1282 | 0.0852 | 0.0166 | 0.0003 | 0.0076 | 0.0058 |
Cytoplasmic Foci | 0.0222 | 0.0165 | 0.0199 | 0.0294 | 0.0148 | 0.0081 | 0.0161 | 0.0093 | 0.0133 | 0.0089 | 0.0132 | 0.0041 | 0.0553 | 0.0121 | 0.0214 | 0.0233 | 0.0097 | 0.0092 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 | 0 | 0.0001 |
Endoplasmic Reticulum | 0.0032 | 0.0002 | 0.0005 | 0 | 0.0003 | 0.0012 | 0.0013 | 0.0001 | 0.0009 | 0.001 | 0.0002 | 0.0004 | 0.0196 | 0.0024 | 0.0207 | 0 | 0.0017 | 0.0075 |
Endosome | 0.0271 | 0.0049 | 0.0211 | 0.0053 | 0.0042 | 0.0093 | 0.0183 | 0.0051 | 0.0123 | 0.0192 | 0.0082 | 0.005 | 0.0674 | 0.004 | 0.0071 | 0.0045 | 0.0535 | 0.012 |
Golgi | 0.01 | 0.0029 | 0.0109 | 0.002 | 0.0054 | 0.0042 | 0.0057 | 0.001 | 0.0044 | 0.0043 | 0.0069 | 0.0017 | 0.0119 | 0.0014 | 0.0038 | 0.0009 | 0.0015 | 0.0023 |
Lipid Particles | 0.1543 | 0.1038 | 0.0944 | 0.1944 | 0.1368 | 0.0494 | 0.0712 | 0.0425 | 0.0526 | 0.0469 | 0.0427 | 0.0321 | 0.1543 | 0.0405 | 0.0672 | 0.034 | 0.0128 | 0.0265 |
Mitochondria | 0.0089 | 0.0005 | 0.0252 | 0.0001 | 0.1045 | 0.0018 | 0.0052 | 0.0002 | 0.0073 | 0.0045 | 0.0109 | 0.0017 | 0.0026 | 0.0003 | 0.0314 | 0.0001 | 0.0007 | 0.0022 |
None | 0.0101 | 0.0026 | 0.0023 | 0.0002 | 0.0004 | 0.0042 | 0.0019 | 0.003 | 0.0021 | 0.0008 | 0.0004 | 0.002 | 0.0158 | 0.0016 | 0.0019 | 0.0013 | 0.0024 | 0.0727 |
Nuclear Periphery | 0.0501 | 0.0193 | 0.0131 | 0.0005 | 0.0434 | 0.1365 | 0.0255 | 0.0036 | 0.0121 | 0.0208 | 0.0012 | 0.084 | 0.0851 | 0.0504 | 0.0052 | 0.0004 | 0.1075 | 0.3301 |
Nucleolus | 0.3253 | 0.4791 | 0.5702 | 0.5347 | 0.1294 | 0.3196 | 0.4314 | 0.5447 | 0.5747 | 0.6319 | 0.2886 | 0.3702 | 0.1595 | 0.5487 | 0.5886 | 0.6629 | 0.4197 | 0.1331 |
Nucleus | 0.0552 | 0.0836 | 0.0481 | 0.0323 | 0.0124 | 0.1089 | 0.0909 | 0.112 | 0.0572 | 0.035 | 0.053 | 0.11 | 0.0241 | 0.0799 | 0.074 | 0.035 | 0.1462 | 0.0692 |
Peroxisomes | 0.015 | 0.0032 | 0.0089 | 0.0048 | 0.0037 | 0.002 | 0.012 | 0.0015 | 0.0053 | 0.0046 | 0.0067 | 0.0024 | 0.0287 | 0.0009 | 0.0053 | 0.0017 | 0.0019 | 0.0018 |
Punctate Nuclear | 0.2819 | 0.2765 | 0.1717 | 0.192 | 0.5098 | 0.3429 | 0.2953 | 0.2576 | 0.2431 | 0.2097 | 0.5386 | 0.3783 | 0.2178 | 0.1651 | 0.134 | 0.2074 | 0.2277 | 0.3087 |
Vacuole | 0.013 | 0.0014 | 0.0029 | 0.0012 | 0.0007 | 0.0023 | 0.0027 | 0.0066 | 0.0029 | 0.0025 | 0.0007 | 0.0013 | 0.0046 | 0.002 | 0.0149 | 0.0096 | 0.0019 | 0.0057 |
Vacuole Periphery | 0.0044 | 0.0006 | 0.0013 | 0.0001 | 0.0011 | 0.0024 | 0.0016 | 0.0007 | 0.0018 | 0.0019 | 0.0004 | 0.0015 | 0.0031 | 0.001 | 0.0007 | 0.0002 | 0.0023 | 0.0068 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.813 | 8.5032 | 5.6087 | 6.5992 | 4.111 | 4.2253 | 5.4929 | 6.7699 | 5.5532 | 6.9676 |
Translational Efficiency | 1.1817 | 0.8192 | 1.2828 | 0.743 | 1.4689 | 1.8221 | 1.1332 | 1.1424 | 1.4456 | 1.2051 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✔ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1545 | 269 | 242 | 831 | 1259 | 1668 | 222 | 1114 | 2804 | 1937 | 464 | 1945 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 734.46 | 851.86 | 945.16 | 857.96 | 634.93 | 749.87 | 990.81 | 888.42 | 689.77 | 764.03 | 967.00 | 875.41 |
Standard Deviation | 89.72 | 109.41 | 91.45 | 114.39 | 84.49 | 95.53 | 108.87 | 127.12 | 100.46 | 103.75 | 102.73 | 122.77 |
Intensity Change Log 2 | — | 0.213932 | 0.363875 | 0.224226 | — | 0.240043 | 0.642011 | 0.484644 | — | 0.226098 | 0.499523 | 0.350834 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000262 | 0.000957 | 0.001227 | 0.000939 | 0.000348 | 0.000497 | 0.001378 | 0.000752 | 0.000301 | 0.000561 | 0.001300 | 0.000832 |
Bud Neck | 0.006283 | 0.017253 | 0.011467 | 0.021934 | 0.005508 | 0.007132 | 0.011000 | 0.021101 | 0.005935 | 0.008537 | 0.011243 | 0.021457 |
Bud Site | 0.001302 | 0.006152 | 0.002638 | 0.006273 | 0.001033 | 0.002232 | 0.003073 | 0.014079 | 0.001181 | 0.002777 | 0.002846 | 0.010744 |
Cell Periphery | 0.000121 | 0.000187 | 0.000089 | 0.000124 | 0.000146 | 0.000141 | 0.000107 | 0.000171 | 0.000132 | 0.000147 | 0.000097 | 0.000151 |
Cytoplasm | 0.032238 | 0.013149 | 0.013036 | 0.025002 | 0.026333 | 0.013386 | 0.016624 | 0.035391 | 0.029587 | 0.013353 | 0.014753 | 0.030952 |
Cytoplasmic Foci | 0.037042 | 0.019776 | 0.000789 | 0.005982 | 0.028478 | 0.023370 | 0.001917 | 0.008172 | 0.033196 | 0.022871 | 0.001329 | 0.007236 |
Eisosomes | 0.000109 | 0.000147 | 0.000095 | 0.000067 | 0.000131 | 0.000118 | 0.000090 | 0.000053 | 0.000119 | 0.000122 | 0.000093 | 0.000059 |
Endoplasmic Reticulum | 0.002617 | 0.008978 | 0.006689 | 0.013537 | 0.002832 | 0.003920 | 0.012567 | 0.006439 | 0.002713 | 0.004622 | 0.009501 | 0.009472 |
Endosome | 0.012923 | 0.016138 | 0.003003 | 0.010958 | 0.008056 | 0.013588 | 0.005322 | 0.016811 | 0.010738 | 0.013942 | 0.004113 | 0.014311 |
Golgi | 0.006711 | 0.005289 | 0.000809 | 0.006169 | 0.004865 | 0.008737 | 0.000989 | 0.004247 | 0.005882 | 0.008259 | 0.000895 | 0.005069 |
Lipid Particles | 0.042958 | 0.032255 | 0.002033 | 0.003515 | 0.035380 | 0.031510 | 0.004487 | 0.003298 | 0.039555 | 0.031613 | 0.003207 | 0.003391 |
Mitochondria | 0.013002 | 0.007267 | 0.001925 | 0.004647 | 0.009431 | 0.015591 | 0.002083 | 0.007846 | 0.011398 | 0.014435 | 0.002001 | 0.006479 |
Mitotic Spindle | 0.031348 | 0.143490* | 0.111366* | 0.277504* | 0.024448 | 0.084584 | 0.140004* | 0.382328* | 0.028250 | 0.092765 | 0.125068* | 0.337542* |
None | 0.017823 | 0.006125 | 0.004260 | 0.005304 | 0.023680 | 0.010255 | 0.003413 | 0.004975 | 0.020453 | 0.009682 | 0.003855 | 0.005116 |
Nuclear Periphery | 0.014540 | 0.016925 | 0.007902 | 0.040626 | 0.010658 | 0.022277 | 0.038399 | 0.028601 | 0.012797 | 0.021533 | 0.022493 | 0.033739 |
Nuclear Periphery Foci | 0.126280 | 0.277944* | 0.080301 | 0.142155 | 0.134372 | 0.178800 | 0.154887 | 0.096193 | 0.129913 | 0.192569* | 0.115987 | 0.115830 |
Nucleolus | 0.497532* | 0.270332* | 0.410929* | 0.230235* | 0.522874* | 0.410679* | 0.332103* | 0.207448 | 0.508911* | 0.391188* | 0.373215* | 0.217184 |
Nucleus | 0.146092* | 0.138181* | 0.331530* | 0.183737* | 0.148123* | 0.158923* | 0.254070* | 0.131726* | 0.147004* | 0.156042* | 0.294469* | 0.153948* |
Peroxisomes | 0.007617 | 0.013703 | 0.003397 | 0.003069 | 0.010817 | 0.010095 | 0.008825 | 0.003364 | 0.009053 | 0.010596 | 0.005994 | 0.003238 |
Vacuole | 0.002287 | 0.004303 | 0.005823 | 0.015302 | 0.001739 | 0.002313 | 0.007842 | 0.021389 | 0.002041 | 0.002589 | 0.006789 | 0.018788 |
Vacuole Periphery | 0.000915 | 0.001448 | 0.000693 | 0.002920 | 0.000748 | 0.001855 | 0.000819 | 0.005617 | 0.000840 | 0.001798 | 0.000753 | 0.004465 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -6.89 | -8.31 | -9.61 | -1.55 | 2.05 | -2.46 | -6.84 | -5.67 | -4.23 | 4.59 | -6.07 | -10.53 | -10.95 | -6.46 | 5.10 |
Bud Neck | -4.52 | -6.72 | -13.36 | -3.73 | -4.11 | -2.23 | -7.73 | -14.76 | -14.33 | -6.20 | -4.23 | -10.04 | -19.90 | -16.98 | -7.18 |
Bud Site | -3.94 | -4.80 | -9.07 | -2.69 | -3.12 | -2.72 | -6.39 | -10.98 | -10.08 | -5.55 | -4.17 | -7.75 | -13.99 | -11.75 | -6.37 |
Cell Periphery | -3.74 | 0.81 | -0.76 | 3.19 | -1.33 | 0.30 | 1.04 | 0.46 | 0.30 | -0.02 | -1.40 | 1.23 | 0.09 | 0.89 | -0.52 |
Cytoplasm | 4.14 | 4.94 | 1.34 | -2.57 | -3.29 | 4.17 | 2.51 | -2.93 | -6.61 | -4.60 | 7.10 | 5.26 | -1.41 | -7.20 | -5.85 |
Cytoplasmic Foci | 4.96 | 15.46 | 13.75 | 4.24 | -3.23 | 2.77 | 12.75 | 9.93 | 8.38 | -4.78 | 5.39 | 20.03 | 16.61 | 10.09 | -5.83 |
Eisosomes | -4.26 | -0.77 | 6.76 | 7.34 | 4.07 | 3.27 | 2.65 | 17.19 | 15.32 | 6.35 | -0.84 | 1.02 | 17.09 | 15.76 | 7.46 |
Endoplasmic Reticulum | -4.80 | -8.38 | -6.98 | -1.67 | -3.03 | -3.59 | -6.39 | -5.64 | -3.95 | 4.22 | -7.22 | -9.15 | -8.89 | -5.72 | 1.37 |
Endosome | -1.60 | 5.39 | 1.11 | 2.37 | -4.44 | -4.05 | 1.60 | -3.04 | 0.36 | -3.98 | -2.66 | 5.31 | -1.15 | 1.44 | -5.93 |
Golgi | 1.58 | 8.72 | 2.03 | 0.93 | -2.14 | -2.91 | 6.14 | 2.97 | 4.94 | -1.82 | -1.94 | 10.62 | 3.66 | 4.40 | -2.85 |
Lipid Particles | 2.25 | 14.38 | 14.17 | 5.96 | -1.56 | 1.13 | 11.24 | 12.45 | 12.94 | 1.15 | 3.09 | 18.17 | 18.82 | 14.14 | 0.31 |
Mitochondria | 4.10 | 10.32 | 7.71 | 2.97 | -4.56 | -3.49 | 5.55 | 2.48 | 6.10 | -3.18 | -2.15 | 11.30 | 6.60 | 7.15 | -4.66 |
Mitotic Spindle | -8.21 | -7.18 | -21.04 | -7.09 | -7.79 | -10.02 | -8.13 | -30.17* | -23.86* | -9.01 | -12.80 | -10.82 | -36.52 | -25.66 | -12.43 |
None | 7.25 | 8.47 | 9.59 | 1.47 | 0.50 | 6.09 | 9.99 | 9.01 | 5.59 | -2.13 | 7.98 | 13.19 | 13.08 | 6.30 | -0.82 |
Nuclear Periphery | -0.88 | 4.33 | -5.68 | -4.30 | -7.75 | -4.88 | -3.22 | -4.74 | -0.02 | 1.14 | -4.46 | -1.84 | -7.17 | -2.32 | -2.79 |
Nuclear Periphery Foci | -7.63 | 4.04 | 0.81 | 7.74 | -3.10 | -4.92 | 0.35 | 7.92 | 13.27 | 3.94 | -8.51 | 2.77 | 6.44 | 13.55 | 1.26 |
Nucleolus | 13.48* | 5.93 | 21.97* | 2.25 | 8.26 | 9.64 | 9.76 | 26.28* | 18.55 | 5.86 | 13.07 | 10.97 | 34.28* | 19.12 | 10.15 |
Nucleus | 0.24 | -9.15 | -4.81 | -3.73 | 6.42 | -2.79 | -6.42 | -0.83 | 1.60 | 5.87 | -2.87 | -11.08 | -3.75 | -1.08 | 8.94 |
Peroxisomes | -2.65 | 4.41 | 8.31 | 4.70 | 1.94 | 0.93 | 1.11 | 8.83 | 8.83 | 2.46 | -1.52 | 2.55 | 11.99 | 10.30 | 2.95 |
Vacuole | -2.90 | -3.36 | -8.55 | -7.47 | -3.38 | -1.08 | -5.06 | -10.21 | -10.09 | -4.40 | -1.74 | -5.71 | -13.28 | -13.01 | -5.52 |
Vacuole Periphery | -2.58 | 0.24 | -2.89 | -1.61 | -3.00 | -2.85 | -2.20 | -4.10 | -2.14 | -3.69 | -2.90 | -1.14 | -4.95 | -2.24 | -4.72 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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