Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0.05 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.05 0.06 0.06 0.06 0.05 0.06 0.06 0.06 – 0.07 0.06 0.09 0.12 0.18 0.17 0.17 0.2 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.07 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.2 0.53 0.54 0.32 0.63 0.62 0.62 0.79 – 0.79 0.8 0 0 0.12 0.08 0 0.05 0 0 0 0 0 0
Nucleus 0.88 0.8 0.71 0.73 0.8 0.68 0.64 0.59 0.57 – 0.55 0.47 0.85 0.83 0.72 0.74 0.7 0.65 0.81 0.82 0.69 0.76 0.67 0.61
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.08 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.05 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 0 0 – 0 0.06 0.05 0 0.05 0.11 0.15 0.12 0.07 0.06 0.15 0.07 0.13 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud 0 0 1 2 2 4 3 15 13 – 16 18 0 0 1 0 0 0 0 2 1 5 9 13
Bud Neck 0 0 0 0 0 0 0 0 0 – 2 1 0 0 1 0 0 1 0 1 0 0 2 4
Bud Site 0 0 3 1 5 17 11 12 22 – 20 27 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cytoplasm 0 10 10 14 23 23 40 54 41 – 27 37 21 46 83 45 30 54 1 2 4 2 9 6
Endoplasmic Reticulum 0 2 1 0 0 0 0 0 0 – 0 0 0 0 1 17 3 15 0 0 0 0 1 3
Endosome 0 0 0 0 7 3 0 2 0 – 0 0 1 3 3 0 5 3 3 4 1 2 4 12
Golgi 0 1 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 1 2 0 0 1 1
Mitochondria 27 43 90 127 132 279 416 586 552 – 319 462 8 9 56 21 5 13 0 4 5 0 5 8
Nucleus 134 173 121 173 325 303 427 561 395 – 224 272 194 313 335 192 121 173 142 193 134 137 242 339
Nuclear Periphery 0 0 0 0 1 2 0 3 1 – 0 3 0 0 1 1 0 0 0 0 1 0 0 1
Nucleolus 12 0 5 5 2 5 4 41 11 – 5 14 0 4 5 1 0 0 9 3 5 12 19 57
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 1 2 2 3 1 4 4 6 – 2 6 0 2 1 4 0 2 4 2 5 6 12 22
Vac/Vac Membrane 3 13 4 8 14 19 17 39 12 – 16 33 11 11 23 29 26 32 12 15 29 11 48 76
Unique Cell Count 152 215 171 237 408 446 671 946 698 406 575 227 376 464 260 172 266 177 236 195 182 363 556
Labelled Cell Count 177 244 237 332 514 656 922 1317 1053 631 873 235 388 510 312 190 293 177 236 195 182 363 556


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.1 4.7 3.8 3.9 4.1 3.7 3.5 3.7 3.2 – 3.2 3.0 5.5 5.5 4.9 6.4 6.9 7.2 5.4 5.3 5.5
Std Deviation (1e-4) 0.6 0.9 0.8 1.3 1.2 0.9 0.9 1.2 0.9 – 0.9 1.2 1.1 1.1 1.1 0.9 1.4 1.3 1.1 1.2 1.1
Intensity Change (Log2) – – – 0.03 0.1 -0.05 -0.11 -0.07 -0.26 – -0.25 -0.35 0.51 0.52 0.36 0.73 0.85 0.9 0.5 0.47 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.4 4.2 3.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.3532 3.7785 3.7149 3.4046 3.1617 3.7524 1.411 1.8709 1.4331 0.887 1.6056 1.408 1.1447 0.0678 -0.0287 0.0582 -0.4099 -0.3649
Actin 0.02 0.0009 0.0045 0.0299 0.0026 0.0002 0.041 0.0001 0.0022 0.0052 0.0015 0.0009 0.12 0.0546 0.0033 0.0001 0.001 0.0002
Bud 0.0008 0.0001 0.0002 0.0001 0.0001 0.0001 0.0006 0.0001 0.0001 0.0002 0.006 0.0004 0.0013 0.001 0 0 0.0002 0.0001
Bud Neck 0.0043 0.0013 0.003 0.001 0.002 0.01 0.0149 0.0004 0.0006 0.0007 0.0045 0.0031 0.0024 0.0062 0.0004 0.0002 0.0017 0.0031
Bud Periphery 0.0015 0.0001 0.0004 0.0001 0.0005 0.0004 0.0009 0.0001 0.0001 0.0004 0.0168 0.0001 0.0029 0.0009 0 0 0.0002 0.0001
Bud Site 0.0076 0.0027 0.0047 0.0005 0.0003 0.0007 0.0046 0.0029 0.0025 0.0003 0.0257 0.0002 0.0272 0.0116 0.0003 0.0001 0.0006 0.0002
Cell Periphery 0.0003 0.0002 0.0002 0 0.0001 0.0002 0.0006 0 0 0 0.0018 0 0.0122 0.0002 0 0 0.0001 0.0001
Cytoplasm 0.0096 0.0175 0.0036 0.0143 0.0017 0.006 0.0137 0.0436 0.0277 0.0168 0.0193 0.0178 0.0661 0.0884 0.0739 0.0331 0.0204 0.0162
Cytoplasmic Foci 0.0047 0.0009 0.0049 0.005 0.0037 0.0002 0.0537 0.0025 0.009 0.001 0.006 0.0113 0.0586 0.0218 0.0034 0.0003 0.0018 0.0003
Eisosomes 0.0003 0.0001 0.0002 0 0.0001 0 0.0014 0 0 0.0001 0 0 0.0017 0.0002 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.004 0.0014 0.0006 0.0002 0.0017 0.0006 0.0086 0.0009 0.0007 0.0006 0.0019 0.0006 0.0251 0.003 0.0011 0.0019 0.0017 0.0004
Endosome 0.0138 0.0011 0.006 0.0037 0.0038 0.0006 0.0305 0.0009 0.0053 0.0081 0.038 0.0118 0.05 0.0169 0.0016 0.0015 0.0022 0.0004
Golgi 0.0052 0.0003 0.0038 0.0015 0.0009 0 0.0065 0 0.0038 0.0073 0.0108 0.0031 0.0336 0.017 0.0002 0 0.0002 0
Lipid Particles 0.0078 0.002 0.0053 0.0136 0.0231 0.0002 0.0253 0 0.0012 0.0024 0.0025 0.0049 0.0386 0.005 0.0005 0.0001 0.0008 0
Mitochondria 0.0137 0.0037 0.0107 0.0013 0.0046 0.0005 0.0028 0.0003 0.0015 0.0228 0.0235 0.0014 0.01 0.004 0.0007 0.0004 0.0009 0.0002
None 0.006 0.0179 0.003 0.0091 0.0007 0.0012 0.0063 0.0197 0.0249 0.0018 0.001 0.0026 0.1893 0.0181 0.1254 0.0177 0.3176 0.0312
Nuclear Periphery 0.0199 0.013 0.0034 0.003 0.0518 0.0022 0.0281 0.0103 0.0084 0.004 0.01 0.0054 0.0162 0.0269 0.01 0.0142 0.0152 0.0071
Nucleolus 0.0128 0.0099 0.0106 0.0052 0.0204 0.0237 0.0153 0.005 0.0043 0.0076 0.0069 0.0087 0.0044 0.0226 0.0056 0.0026 0.0192 0.0092
Nucleus 0.8154 0.9075 0.9138 0.8908 0.8562 0.9325 0.6756 0.891 0.8959 0.9122 0.6995 0.8869 0.2838 0.5808 0.7106 0.9038 0.5861 0.9166
Peroxisomes 0.003 0.0053 0.0045 0.0081 0.0152 0.0001 0.0177 0 0.0033 0.0011 0.002 0.0089 0.0354 0.0051 0.0008 0 0.0002 0
Punctate Nuclear 0.0441 0.0114 0.0151 0.012 0.0076 0.0186 0.0472 0.0208 0.0072 0.0064 0.0688 0.0307 0.0111 0.1105 0.0611 0.0215 0.0284 0.0137
Vacuole 0.0031 0.0024 0.001 0.0006 0.0011 0.0015 0.0028 0.001 0.001 0.0004 0.0353 0.0007 0.0078 0.0047 0.0008 0.0019 0.0011 0.0007
Vacuole Periphery 0.0021 0.0003 0.0004 0.0001 0.0017 0.0001 0.0018 0.0002 0.0001 0.0006 0.0179 0.0003 0.0022 0.0008 0.0002 0.0004 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.6475 13.8828 13.7356 13.557 10.0676 9.5715 15.5576 19.1848 15.1657 13.1967
Translational Efficiency 0.4215 0.3518 0.4162 0.351 0.3305 0.5525 0.3742 0.2864 0.4071 0.3687

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1225 1779 1472 878 1379 1457 109 1949 2604 3236 1581 2827

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 655.79 787.61 1025.10 857.17 683.24 683.59 965.01 799.93 670.33 740.78 1020.96 817.71
Standard Deviation 105.49 99.51 127.20 118.43 81.96 152.26 103.24 106.00 94.76 136.23 126.61 113.16
Intensity Change Log 2 — 0.264248 0.644459 0.386347 — 0.000739 0.498151 0.227481 — 0.135805 0.571660 0.307472

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000150 0.000242 0.000771 0.001345 0.000188 0.000197 0.000861 0.000798 0.000170 0.000222 0.000777 0.000968
Bud Neck 0.001428 0.004591 0.013879 0.024086 0.001854 0.008550 0.001564 0.022253 0.001654 0.006374 0.013030 0.022822
Bud Site 0.001045 0.000667 0.003185 0.021551 0.000580 0.003672 0.003138 0.011348 0.000799 0.002020 0.003182 0.014517
Cell Periphery 0.000188 0.000066 0.000111 0.000218 0.000113 0.000553 0.000115 0.000164 0.000148 0.000285 0.000112 0.000181
Cytoplasm 0.262759* 0.033447 0.006715 0.096677 0.045535 0.091861 0.019897 0.108406* 0.147724* 0.059748 0.007624 0.104763*
Cytoplasmic Foci 0.015038 0.002532 0.000193 0.004317 0.003789 0.015248 0.001666 0.001980 0.009081 0.008257 0.000295 0.002706
Eisosomes 0.000025 0.000016 0.000023 0.000031 0.000019 0.000032 0.000064 0.000025 0.000022 0.000023 0.000026 0.000027
Endoplasmic Reticulum 0.001214 0.002724 0.005416 0.006811 0.001959 0.002289 0.007892 0.003849 0.001608 0.002528 0.005587 0.004769
Endosome 0.000753 0.000917 0.000251 0.006716 0.000329 0.002644 0.000670 0.002553 0.000529 0.001695 0.000280 0.003846
Golgi 0.000227 0.000577 0.000065 0.009373 0.000113 0.001091 0.000072 0.001805 0.000167 0.000809 0.000066 0.004156
Lipid Particles 0.001498 0.000718 0.000159 0.000630 0.000925 0.002796 0.001299 0.000411 0.001195 0.001654 0.000237 0.000479
Mitochondria 0.001019 0.001452 0.001326 0.005248 0.001419 0.008102 0.000578 0.005746 0.001231 0.004446 0.001274 0.005592
Mitotic Spindle 0.000988 0.004239 0.002033 0.038992 0.001251 0.005748 0.000291 0.023725 0.001127 0.004918 0.001913 0.028466
None 0.055611 0.006460 0.002343 0.007862 0.011020 0.031035 0.002040 0.008800 0.031997 0.017525 0.002322 0.008509
Nuclear Periphery 0.000470 0.001212 0.000874 0.002788 0.000832 0.003765 0.000653 0.002559 0.000662 0.002361 0.000859 0.002630
Nuclear Periphery Foci 0.002055 0.000818 0.000161 0.000953 0.000472 0.001095 0.000536 0.000380 0.001217 0.000943 0.000187 0.000558
Nucleolus 0.007551 0.005479 0.002299 0.001078 0.006678 0.011957 0.002905 0.001765 0.007089 0.008396 0.002341 0.001552
Nucleus 0.643094* 0.931452* 0.956845* 0.735122* 0.921327* 0.800444* 0.943120* 0.785702* 0.790438* 0.872466* 0.955899* 0.769993*
Peroxisomes 0.000452 0.000523 0.000113 0.000683 0.000578 0.002110 0.000339 0.000338 0.000519 0.001238 0.000129 0.000445
Vacuole 0.004160 0.001537 0.003070 0.027433 0.000894 0.006009 0.012165 0.015232 0.002430 0.003551 0.003697 0.019021
Vacuole Periphery 0.000275 0.000330 0.000166 0.008086 0.000124 0.000800 0.000134 0.002162 0.000195 0.000542 0.000164 0.004002

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.60 -17.00 -5.52 -4.97 -1.42 -0.04 -5.46 -2.21 -2.21 2.23 -2.46 -17.51 -4.36 -3.99 1.02
Bud Neck -7.48 -16.09 -9.14 -7.45 -1.49 -6.91 -1.99 -14.29 -7.55 -12.72 -9.56 -15.97 -17.04 -11.79 -3.37
Bud Site 1.90 -3.04 -6.34 -6.53 -5.07 -4.65 -1.47 -7.01 -3.40 -1.80 -3.95 -3.67 -9.36 -7.66 -5.89
Cell Periphery 5.09 3.23 0.39 -6.71 -3.62 -1.86 -1.09 -0.57 1.82 0.84 -1.28 2.48 0.59 1.37 -2.44
Cytoplasm 29.24 34.05 23.29 -6.88 -14.68 -9.03 4.54 -9.12 0.82 -10.83 17.75 31.47 13.10 -5.89 -26.55
Cytoplasmic Foci 8.87 10.80 9.29 1.21 -4.13 -7.21 3.21 3.60 9.66 0.09 1.30 11.85 10.00 9.51 -6.29
Eisosomes 5.03 -0.08 -7.39 -11.85 -8.21 -5.15 -5.78 -5.45 1.19 4.88 -0.73 -4.61 -8.15 -7.39 -3.12
Endoplasmic Reticulum -7.23 -21.24 -18.13 -14.52 -8.54 -0.97 -6.43 -10.94 -8.98 4.40 -5.25 -19.88 -20.85 -15.90 -1.25
Endosome -0.24 5.16 -3.03 -2.66 -3.79 -5.58 -2.91 -4.05 2.19 -0.83 -4.19 3.44 -4.68 -0.75 -5.37
Golgi -0.99 5.11 -2.01 -1.74 -2.12 -3.10 0.89 -1.69 0.54 -1.76 -2.78 4.35 -2.57 -1.24 -2.74
Lipid Particles 4.07 7.00 3.40 -1.40 -8.88 -4.93 -1.84 3.96 6.66 3.76 -1.68 8.43 5.61 6.29 -7.48
Mitochondria -1.91 -4.60 -4.71 -3.62 -3.75 -5.21 1.56 -3.63 2.92 -5.78 -5.30 -0.94 -5.36 0.66 -5.56
Mitotic Spindle -2.39 -1.43 -6.45 -5.35 -5.94 -2.94 0.85 -7.42 -4.84 -7.81 -3.71 -1.34 -9.85 -7.31 -8.58
None 14.17 15.63 14.58 1.02 -7.01 -6.67 7.70 2.88 8.41 -9.26 6.65 16.70 13.93 7.79 -10.68
Nuclear Periphery -6.49 -8.27 -11.85 -9.36 -9.83 -3.35 -1.51 -8.42 0.29 -6.65 -4.29 -6.31 -13.24 -3.29 -10.92
Nuclear Periphery Foci 3.58 7.20 2.06 -1.86 -5.53 -1.90 -1.61 1.17 2.16 2.12 0.90 7.24 3.69 1.24 -5.25
Nucleolus 2.69 6.68 10.05 9.64 7.28 -3.74 4.88 6.61 8.91 1.12 -1.54 7.69 11.00 11.78 5.28
Nucleus -29.98* -33.65 -11.22 15.16 18.15 14.34 -0.10 15.12 -1.73 7.04 -12.19 -26.95 -2.31 10.72 27.50
Peroxisomes 0.18 3.85 1.67 1.31 -2.44 -4.42 1.95 3.01 6.25 2.22 -3.58 5.01 3.68 6.30 -2.26
Vacuole 3.76 0.63 -11.52 -12.32 -11.70 -5.76 -3.33 -14.79 -10.01 0.62 -1.82 -3.76 -17.51 -16.68 -14.39
Vacuole Periphery -0.42 1.65 -2.38 -2.31 -2.50 -4.52 -0.99 -2.61 -0.68 -2.49 -3.45 0.92 -3.42 -2.50 -3.52
Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
Localization
Cell Percentages nucleus (85%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

Ssn2

Ssn2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ssn2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available