ORF
Human Ortholog
Description Putative hydrolase acting on ester bonds
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.09 0.08 0 0 0 – 0 0 0 0.05 0 0 0 0.05 0.06 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.98 1.0 0.97 0.96 0.9 – 0.85 0.83 0.75 0.78 0.98 0.98 0.99 0.94 0.96 0.98 0.92 0.9 0.78 0.87 0.9 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.08 0.1 – 0.19 0.18 0.39 0.22 0 0 0 0 0 0 0 0 0.07 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 2 0 0 0 1 1 – 1 3 1 2 2 0 1 0 0 0 2 6 7 1 0 4
Bud 2 0 0 0 0 0 2 – 5 4 10 5 1 0 0 0 0 0 1 1 1 1 6 4
Bud Neck 0 1 7 0 0 2 4 – 1 4 1 2 3 1 2 1 1 0 1 7 3 0 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 1 1 0 0 0 0 1 0 – – – – – –
Cell Periphery 2 15 15 10 8 7 12 – 6 6 12 16 2 6 7 26 26 16 1 8 7 0 0 0
Cytoplasm 185 421 172 123 194 242 255 – 193 208 223 242 318 443 417 478 399 358 168 382 138 174 375 349
Endoplasmic Reticulum 2 3 1 0 2 0 4 – 1 6 8 4 2 3 2 27 17 20 0 1 0 1 2 3
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 1 3 0 5 3 0 2 1 0 1 3 3
Golgi 0 0 0 0 1 0 0 – 0 0 0 0 1 0 0 2 3 0 0 2 0 0 1 1
Mitochondria 2 1 0 1 7 19 27 – 43 45 115 69 2 0 0 12 6 0 0 2 12 3 7 3
Nucleus 0 0 0 0 0 0 2 – 2 1 2 2 0 2 1 3 3 2 0 0 0 0 1 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 2 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 0 0 0 0 0 0 6 – 1 0 1 9 0 1 1 3 1 0 0 0 0 0 1 2
Vac/Vac Membrane 0 1 0 0 3 1 2 – 0 2 1 3 0 0 0 8 13 3 0 2 1 3 2 1
Unique Cell Count 188 429 175 123 201 253 284 226 252 297 311 324 450 420 506 417 365 183 423 177 200 418 384
Labelled Cell Count 193 444 195 134 215 272 315 253 279 375 355 332 459 431 565 473 400 183 423 177 200 418 384


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.7 5.6 5.7 5.8 4.6 4.7 – 4.3 4.3 4.0 4.4 6.2 6.1 6.2 6.9 7.2 7.0 5.8 6.0 6.1
Std Deviation (1e-4) 0.8 0.6 0.7 1.5 1.7 0.8 1.0 – 1.1 0.6 1.1 1.5 1.1 1.6 1.4 1.1 1.2 1.4 1.2 1.3 1.3
Intensity Change (Log2) – – – 0.02 0.03 -0.28 -0.28 – -0.4 -0.4 -0.48 -0.36 0.14 0.11 0.13 0.29 0.36 0.32 0.03 0.1 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 -1.9 0 0 – 0 0 0 0 -4.7 -4.5 -4.1 -1.6 -1.0 -2.0
Cytoplasm 1.5 -1.1 -1.5 -3.5 – -4.5 -5.1 -6.6 -6.1 -0.1 0.1 1.1 -2.1 -1.6 -0.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 2.7 2.3 2.8
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0507 0.0815 0.0711 0.5195 0.2628 0.0046 0.7935 0.9482 0.9476 0.6949 0.5506 0.9404 0.6242 0.6802 0.601 0.9086 0.5681 0.5654
Actin 0.0311 0.0042 0.01 0.0182 0.0173 0.0036 0.0272 0.0054 0.0051 0.003 0.0011 0.0045 0.033 0.0014 0.0126 0.0101 0.0173 0.0086
Bud 0.0011 0.0003 0.0069 0.002 0.0024 0.0003 0.0034 0.0091 0.0053 0.0036 0.0005 0.0039 0.0021 0.0024 0.0026 0.0018 0.0034 0.0007
Bud Neck 0.0013 0.0011 0.0022 0.0027 0.0012 0.0039 0.0056 0.0005 0.0012 0.0004 0.0007 0.0058 0.0052 0.0008 0.0012 0.0034 0.0022 0.0022
Bud Periphery 0.0012 0.0004 0.0119 0.0038 0.0046 0.0013 0.0072 0.0041 0.0077 0.0143 0.0011 0.0022 0.0057 0.0019 0.0033 0.0029 0.0094 0.0012
Bud Site 0.0056 0.0123 0.018 0.005 0.011 0.0003 0.0356 0.023 0.0103 0.0005 0.0004 0.0008 0.0087 0.0194 0.014 0.0034 0.0053 0.0014
Cell Periphery 0.001 0.0017 0.0013 0.0015 0.0006 0.0005 0.0023 0.0016 0.0004 0.0008 0.0003 0.0002 0.0007 0.0007 0.0006 0.0003 0.0004 0.0002
Cytoplasm 0.315 0.362 0.3898 0.4428 0.4133 0.5013 0.2974 0.372 0.2694 0.2859 0.3672 0.3186 0.4807 0.5459 0.472 0.582 0.6256 0.6131
Cytoplasmic Foci 0.0332 0.0147 0.0172 0.0299 0.0255 0.0091 0.0107 0.0046 0.0129 0.0029 0.0103 0.0049 0.0096 0.0065 0.0081 0.0431 0.0084 0.0108
Eisosomes 0.0007 0.0005 0.0005 0.0002 0.0003 0.0001 0.0007 0.0003 0.0003 0.0001 0.0002 0.0001 0.0005 0.0001 0.0007 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.0064 0.0036 0.0052 0.0055 0.0056 0.0066 0.0056 0.0025 0.0041 0.0054 0.0021 0.0009 0.006 0.0024 0.0036 0.0053 0.0029 0.002
Endosome 0.0162 0.0073 0.0058 0.0262 0.0208 0.0112 0.0194 0.001 0.0051 0.0018 0.0007 0.0015 0.0119 0.0009 0.0041 0.0205 0.0032 0.0026
Golgi 0.0063 0.0017 0.0027 0.0309 0.0094 0.0042 0.005 0.0003 0.0045 0.0002 0.0002 0.0015 0.005 0.0002 0.002 0.0058 0.0009 0.0008
Lipid Particles 0.0193 0.0006 0.0029 0.0119 0.0077 0.0018 0.008 0.0003 0.004 0.0004 0.0002 0.0011 0.0056 0.0003 0.0054 0.0072 0.0003 0.0025
Mitochondria 0.0069 0.0003 0.0102 0.0292 0.057 0.0031 0.0149 0.0006 0.0083 0.0007 0.0005 0.003 0.0058 0.0002 0.0069 0.0019 0.0018 0.001
None 0.5103 0.5841 0.4962 0.3529 0.3407 0.4443 0.5216 0.5631 0.6312 0.6716 0.5979 0.6387 0.3926 0.4084 0.4491 0.2836 0.3029 0.3378
Nuclear Periphery 0.0057 0.0005 0.0027 0.001 0.0073 0.0026 0.0043 0.0008 0.0028 0.0031 0.0004 0.0009 0.0065 0.0006 0.0021 0.0048 0.0034 0.0011
Nucleolus 0.0011 0.0002 0.0009 0.0013 0.0019 0.0002 0.0018 0.0006 0.0007 0.0002 0.0005 0.0003 0.0005 0.0003 0.0005 0.0009 0.0005 0.0006
Nucleus 0.0047 0.0009 0.0031 0.0013 0.0442 0.0011 0.0068 0.0054 0.0045 0.0014 0.0014 0.0032 0.004 0.0035 0.0026 0.0096 0.0057 0.0026
Peroxisomes 0.0141 0.0003 0.0057 0.0268 0.0183 0.0015 0.0088 0.0002 0.0169 0.0002 0.0011 0.0044 0.0094 0.0002 0.0041 0.0028 0.0008 0.0049
Punctate Nuclear 0.0078 0.0009 0.0046 0.0012 0.0075 0.001 0.0047 0.0037 0.0036 0.0006 0.0128 0.0028 0.0024 0.0027 0.0032 0.0081 0.0038 0.0051
Vacuole 0.0072 0.0021 0.0018 0.0048 0.0024 0.0017 0.008 0.0008 0.001 0.002 0.0003 0.0004 0.0032 0.001 0.001 0.0018 0.0012 0.0007
Vacuole Periphery 0.0039 0.0002 0.0004 0.0009 0.001 0.0006 0.0011 0.0001 0.0005 0.0008 0 0.0001 0.0009 0.0001 0.0003 0.0005 0.0004 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.7611 12.4253 9.2201 9.2695 17.6747 5.343 13.288 9.7838 10.5546 13.1877
Translational Efficiency 0.4039 0.3715 0.3582 0.399 0.218 0.7962 0.4226 0.5076 0.3735 0.356

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
281 1741 716 1152 1482 2703 820 683 1763 4444 1536 1835

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 697.75 769.44 900.02 806.69 663.26 745.38 865.94 807.82 668.76 754.81 881.83 807.11
Standard Deviation 70.65 110.82 580.29 106.85 73.05 95.74 97.92 103.70 73.76 102.59 402.96 105.69
Intensity Change Log 2 — 0.141099 0.367247 0.209304 — 0.168402 0.384693 0.284459 — 0.154469 0.375775 0.246419

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000045 0.000293 0.000328 0.000939 0.000165 0.000576 0.000252 0.002916 0.000146 0.000465 0.000288 0.001675
Bud Neck 0.005252 0.012502 0.004766 0.010583 0.009501 0.032486 0.003486 0.006150 0.008824 0.024657 0.004082 0.008933
Bud Site 0.001221 0.012112 0.011259 0.046476 0.004580 0.017028 0.007655 0.026204 0.004044 0.015102 0.009335 0.038930
Cell Periphery 0.000416 0.000184 0.000347 0.000287 0.000237 0.000194 0.000291 0.000272 0.000265 0.000190 0.000317 0.000281
Cytoplasm 0.550501* 0.590616* 0.420330* 0.504364* 0.557830* 0.565275* 0.547991* 0.498755* 0.556662* 0.575202* 0.488482* 0.502276*
Cytoplasmic Foci 0.217285* 0.151486 0.025764 0.026445 0.176264 0.155306 0.034103 0.043826 0.182803* 0.153810 0.030216 0.032914
Eisosomes 0.000432 0.000140 0.000078 0.000081 0.000248 0.000278 0.000092 0.000098 0.000277 0.000224 0.000085 0.000087
Endoplasmic Reticulum 0.000610 0.001036 0.004380 0.002516 0.000657 0.000702 0.001732 0.001745 0.000650 0.000833 0.002966 0.002229
Endosome 0.009313 0.008921 0.003089 0.005401 0.006117 0.009271 0.002602 0.011617 0.006627 0.009134 0.002829 0.007714
Golgi 0.000943 0.001580 0.000138 0.003863 0.000940 0.002741 0.000165 0.001557 0.000941 0.002286 0.000153 0.003004
Lipid Particles 0.007794 0.004023 0.002136 0.001318 0.005905 0.003040 0.001633 0.003941 0.006206 0.003425 0.001867 0.002294
Mitochondria 0.001519 0.001540 0.000422 0.001129 0.001521 0.002605 0.000303 0.001812 0.001521 0.002188 0.000359 0.001383
Mitotic Spindle 0.001040 0.001637 0.002251 0.025567 0.001627 0.004816 0.002460 0.023111 0.001533 0.003571 0.002363 0.024652
None 0.002410 0.005017 0.002403 0.003575 0.003979 0.001884 0.004140 0.006537 0.003729 0.003111 0.003330 0.004678
Nuclear Periphery 0.000438 0.000226 0.001353 0.000937 0.000229 0.000144 0.000617 0.000895 0.000262 0.000176 0.000960 0.000921
Nuclear Periphery Foci 0.001079 0.000991 0.002396 0.001389 0.000572 0.000241 0.001978 0.001695 0.000653 0.000535 0.002173 0.001503
Nucleolus 0.002898 0.000780 0.000267 0.000366 0.000562 0.000582 0.000239 0.000831 0.000935 0.000660 0.000252 0.000539
Nucleus 0.154340* 0.137317* 0.427947* 0.224176* 0.176164* 0.117561 0.270122* 0.176602* 0.172686* 0.125301 0.343692* 0.206469*
Peroxisomes 0.001954 0.003525 0.000243 0.001638 0.002453 0.005942 0.001138 0.002451 0.002373 0.004995 0.000721 0.001941
Vacuole 0.038578 0.065309 0.089221 0.137341 0.050136 0.078545 0.118897 0.187144 0.048294 0.073360 0.105064 0.155878
Vacuole Periphery 0.001933 0.000763 0.000883 0.001611 0.000311 0.000782 0.000102 0.001840 0.000569 0.000774 0.000466 0.001696

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.72 -9.32 -6.32 -4.13 -3.14 -5.40 -1.09 -1.41 -0.99 -1.33 -6.49 -3.92 -2.53 -1.65 -2.04
Bud Neck -5.41 0.05 -3.99 1.77 -4.41 -12.52 5.52 2.71 14.77 -2.65 -11.88 5.16 0.34 12.86 -5.50
Bud Site -7.84 -4.97 -9.14 -5.09 -5.04 -9.44 -1.15 -4.31 -0.36 -3.39 -10.66 -3.37 -9.14 -4.10 -6.48
Cell Periphery 6.60 1.29 5.18 -2.94 5.15 2.10 -1.68 1.30 -0.33 3.00 4.18 -2.42 2.32 -2.30 5.68
Cytoplasm -2.85 11.02 11.37 19.82 -0.33 -1.07 6.78 12.29 13.46 5.29 -2.76 13.88 19.28 23.91 4.41
Cytoplasmic Foci 6.80 21.47 21.75 29.41 0.41 3.90 29.84 27.92 29.51 -0.73 6.16 36.37 36.61 43.75 0.52
Eisosomes 6.94 8.84 8.91 2.52 0.23 -1.38 10.65 11.31 10.74 0.75 3.20 13.99 14.55 10.25 0.94
Endoplasmic Reticulum -1.49 -11.04 -10.00 -10.79 4.37 -0.29 -9.56 -8.32 -7.76 0.07 -1.60 -15.10 -16.11 -14.41 2.68
Endosome 0.41 3.74 3.50 6.12 -0.13 -4.50 7.06 -1.72 0.57 -4.45 -4.00 7.46 1.69 5.07 -3.66
Golgi -1.68 2.77 -0.59 0.13 -1.70 -7.28 6.14 0.69 5.38 -1.92 -7.05 7.01 -0.46 2.05 -2.10
Lipid Particles 3.99 5.91 6.84 5.07 2.90 4.60 6.57 3.18 0.17 -1.49 4.98 7.44 6.60 3.48 -0.34
Mitochondria -0.01 1.63 1.01 2.69 -3.46 -1.58 1.94 0.67 4.64 -3.26 -1.15 2.10 1.04 6.20 -4.93
Mitotic Spindle -0.57 -0.71 -6.38 -6.39 -6.13 -2.49 -0.13 -4.88 -3.92 -4.72 -2.21 -0.37 -7.91 -7.07 -7.71
None -2.42 0.51 -2.04 1.40 -3.43 2.67 0.72 -0.53 -2.84 -1.19 0.89 1.30 -0.32 -1.67 -2.20
Nuclear Periphery 1.48 -8.00 -8.58 -16.14 -2.16 5.63 -14.71 -14.00 -15.01 -7.42 3.38 -21.04 -19.59 -21.73 -7.04
Nuclear Periphery Foci 0.68 -0.34 -0.14 -4.97 0.92 4.09 -5.14 -4.79 -5.89 -0.94 1.16 -4.54 -4.56 -8.79 -0.21
Nucleolus 1.14 1.40 1.23 1.05 -1.99 0.29 3.01 -1.65 -1.85 -2.89 1.05 2.37 0.38 -1.12 -3.58
Nucleus 1.94 -22.19 -9.14 -14.45 15.64 12.00 -12.66 -1.71 -9.11 9.05 11.07 -24.35 -8.44 -18.25 15.92
Peroxisomes -2.33 4.13 1.21 3.50 -3.06 -7.32 2.59 0.75 6.16 -1.69 -6.75 4.83 2.25 7.86 -2.50
Vacuole -7.69 -12.09 -25.94 -23.08 -14.05 -9.91 -15.64 -22.67 -20.02 -9.96 -11.06 -18.89 -33.83 -30.13 -15.97
Vacuole Periphery 0.87 0.66 0.92 0.26 0.53 -4.57 5.80 -1.57 -0.15 -2.23 -0.89 0.19 -0.48 0.17 -0.64
ORF
Human Ortholog
Description Putative hydrolase acting on ester bonds
Localization
Cell Percentages cytoplasm (56%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-10

YDR444W

YDR444W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ydr444w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available