Standard name
Human Ortholog
Description Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.06 0.05 0.08 0.06 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.98 0.93 0.86 0.79 0.75 0.67 0.73 0.7 0.99 0.98 0.99 0.98 0.96 1.0 0.95 0.94 0.96 0.92 0.93 0.94
Endoplasmic Reticulum 0 0 0 0 0.05 0.1 0.16 0.18 0.2 0.18 0.17 0 0 0 0.05 0.05 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0.09 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 6 4 19 26 11 14 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 2 2 0 1 3 1 3 0 1 0 0 0 1 0 0 0 0 2 1 0 3 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 2 6 3 9 7 24 22 34 15 22 2 7 3 2 1 1 0 0 0 0 0 0
Cytoplasm 421 462 408 230 258 242 331 334 299 196 198 381 366 436 236 149 93 400 434 395 173 387 370
Endoplasmic Reticulum 5 3 2 3 14 28 67 78 88 47 49 7 4 6 13 8 8 5 4 7 4 10 10
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 1 0 0 0 1 2 5 9 42 13 14 0 2 0 0 2 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 4 7 8 4 0 0 0 0 0 1 1 0 0 0 3 0
Nucleus 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 3 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Nucleolus 0 0 0 0 0 0 0 2 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 2 1 1 5 4 3 2 1 0 0 0 2 3 0 4 10 3 3 3 2
Unique Cell Count 425 467 410 234 278 282 421 444 443 267 282 386 373 442 240 155 93 422 462 414 189 415 396
Labelled Cell Count 427 469 418 238 284 289 438 476 501 293 302 390 379 446 253 163 103 422 462 414 189 415 396


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 145.7 123.9 124.9 106.0 80.7 51.5 56.0 45.7 43.9 41.5 40.3 145.5 136.2 128.8 215.9 225.3 226.8 129.2 142.1 145.9
Std Deviation (1e-4) 31.2 28.2 25.0 20.1 18.1 13.7 12.9 10.1 9.2 9.9 10.2 30.4 30.8 27.6 71.0 78.7 72.0 39.0 38.3 39.0
Intensity Change (Log2) -0.24 -0.63 -1.28 -1.16 -1.45 -1.51 -1.59 -1.63 0.22 0.12 0.04 0.79 0.85 0.86 0.05 0.19 0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 4.2 5.0 0 4.6 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1.6 1.0 3.3 2.9 4.3 3.0 4.2 0 0.5 0 0 0 0
Cytoplasm -1.6 -4.9 -7.4 -9.6 -10.5 -12.4 -10.7 -11.5 -1.2 -1.8 -1.3 -1.5 -3.0 0.7
Endoplasmic Reticulum 0 3.9 6.0 8.1 8.6 9.2 8.4 8.4 0 0 0 4.0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 6.4 4.5 4.6 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 109.1623 139.2204 125.529 113.6695 104.2805 106.7913 116.4421 163.0321 151.8447 125.8021 146.3801 113.3015 139.1471 180.98 165.3 160.8971 159.3891 117.247
Actin 0.0163 0.0006 0.0073 0.0441 0.002 0.0033 0.0052 0.0009 0.0067 0.0129 0.0003 0.0026 0.004 0.0004 0.0078 0.0008 0.0003 0.0024
Bud 0.002 0.0008 0.002 0.0013 0.0034 0.0037 0.002 0.0012 0.0041 0.0059 0.0014 0.0013 0.0022 0.001 0.0016 0.002 0.0019 0.0047
Bud Neck 0.0014 0.0006 0.0027 0.0027 0.0011 0.0033 0.001 0.0005 0.0006 0.0007 0.0004 0.0019 0.0012 0.0006 0.001 0.0014 0.0012 0.0018
Bud Periphery 0.0025 0.0006 0.0017 0.0016 0.0019 0.0035 0.0051 0.0006 0.005 0.0131 0.001 0.0006 0.003 0.0007 0.0012 0.0015 0.0007 0.0087
Bud Site 0.0036 0.0007 0.0046 0.0013 0.001 0.0006 0.0012 0.0017 0.0006 0.0026 0.0001 0.0004 0.0012 0.0005 0.0008 0.0004 0.0003 0.0011
Cell Periphery 0.0434 0.0301 0.0404 0.0597 0.0133 0.0159 0.0363 0.0165 0.0272 0.0186 0.0042 0.0025 0.0234 0.0127 0.0298 0.0122 0.0251 0.0419
Cytoplasm 0.4803 0.8475 0.6925 0.4952 0.6667 0.6285 0.66 0.9148 0.8286 0.5169 0.6559 0.6986 0.6597 0.8921 0.7624 0.7057 0.7805 0.4461
Cytoplasmic Foci 0.0144 0.0012 0.0059 0.0097 0.0115 0.0056 0.0115 0.0029 0.0017 0.0041 0.0017 0.0339 0.0039 0.0011 0.0048 0.0029 0.0028 0.0032
Eisosomes 0.0009 0 0.0023 0.001 0.0003 0 0.0005 0 0 0.0014 0 0.0001 0.0005 0 0.0001 0.0001 0 0.0003
Endoplasmic Reticulum 0.0548 0.0188 0.0147 0.0027 0.0034 0.0347 0.0667 0.0051 0.0035 0.0144 0.0011 0.0055 0.0392 0.0069 0.01 0.0106 0.0149 0.0224
Endosome 0.0138 0.0029 0.0091 0.0082 0.0161 0.0362 0.0101 0.0029 0.0062 0.012 0.0086 0.0182 0.0087 0.0033 0.0089 0.0087 0.0084 0.0189
Golgi 0.0061 0.0007 0.0037 0.0038 0.002 0.0289 0.0019 0.0007 0.0018 0.0084 0.0002 0.0058 0.0023 0.0006 0.0017 0.0007 0.0005 0.004
Lipid Particles 0.011 0.0016 0.0085 0.0134 0.005 0.0011 0.004 0.0009 0.0042 0.0042 0.0004 0.0038 0.0049 0.0008 0.0031 0.0019 0.0025 0.0016
Mitochondria 0.0166 0.0017 0.0096 0.0025 0.0171 0.0026 0.0092 0.0014 0.0029 0.0403 0.0001 0.0105 0.0129 0.0009 0.003 0.0025 0.0007 0.0962
None 0.0069 0.0004 0.0047 0.0012 0.0211 0.0008 0.006 0.0004 0.0005 0.0094 0.0001 0.0065 0.007 0.0002 0.002 0.0086 0.0004 0.0017
Nuclear Periphery 0.0534 0.0058 0.0154 0.0078 0.029 0.0425 0.0503 0.0027 0.0072 0.0153 0.003 0.0696 0.0237 0.0037 0.0093 0.0069 0.0122 0.0184
Nucleolus 0.0091 0.0006 0.0018 0.0023 0.0409 0.0016 0.001 0.0005 0.0003 0.0158 0.0004 0.0509 0.0174 0.0001 0.0044 0.0014 0.0009 0.0129
Nucleus 0.0156 0.0027 0.0046 0.0059 0.0225 0.0116 0.009 0.0023 0.0028 0.0109 0.0023 0.0445 0.0176 0.0024 0.0053 0.0052 0.0059 0.0205
Peroxisomes 0.0048 0.0001 0.0021 0.0357 0.0009 0.0001 0.0002 0.0001 0.0004 0.0007 0 0.0007 0.0006 0 0.0004 0.0002 0.0001 0.0004
Punctate Nuclear 0.0055 0 0.0003 0.003 0.0016 0.0001 0.0012 0 0.0008 0.0004 0 0.0058 0.0011 0 0.0032 0.0001 0.0001 0.0001
Vacuole 0.1761 0.0716 0.139 0.253 0.1024 0.143 0.0995 0.0407 0.0859 0.2519 0.2467 0.0201 0.1337 0.063 0.1198 0.1919 0.1175 0.1851
Vacuole Periphery 0.0615 0.011 0.0272 0.0438 0.0365 0.0322 0.0182 0.0032 0.009 0.04 0.072 0.0162 0.0317 0.0088 0.0194 0.0346 0.0233 0.1075

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1600.0707 1838.3556 2256.3503 1609.9767 1923.1464 1886.2869 1473.54 1310.2218 1629.0241 1422.3154
Translational Efficiency 1.5638 1.4477 1.2561 1.7839 1.5578 1.4049 1.5458 1.9035 1.54 1.8538

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
688 1505 1758 2195 1423 2457 2931 1812 2111 3962 4689 4007

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 5262.15 8286.21 10734.19 9242.88 6665.61 8238.97 12585.56 11779.17 6208.21 8256.91 11891.44 10389.81
Standard Deviation 1246.94 1782.85 2177.75 2902.52 1371.40 1822.74 2292.41 2678.92 1485.69 1807.84 2422.03 3074.70
Intensity Change Log 2 0.655060 1.028489 0.812690 0.305727 0.916961 0.821429 0.470343 0.967229 0.817580

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000198 0.000089 0.000077 0.000212 0.000046 0.000056 0.000063 0.000084 0.000096 0.000068 0.000068 0.000154
Bud Neck 0.000295 0.000587 0.001059 0.001511 0.000291 0.000209 0.000274 0.000560 0.000292 0.000353 0.000568 0.001081
Bud Site 0.002569 0.003145 0.002332 0.008204 0.001323 0.004134 0.002673 0.006624 0.001729 0.003758 0.002545 0.007490
Cell Periphery 0.025476 0.015371 0.008017 0.008133 0.026794 0.008335 0.022183 0.009668 0.026365 0.011008 0.016872 0.008827
Cytoplasm 0.316443* 0.689461* 0.664501* 0.647355* 0.634477* 0.811940* 0.759772* 0.763490* 0.530826* 0.765415* 0.724053* 0.699872*
Cytoplasmic Foci 0.014201 0.011585 0.011810 0.005348 0.006866 0.011208 0.004668 0.009150 0.009257 0.011351 0.007345 0.007067
Eisosomes 0.000003 0.000002 0.000002 0.000003 0.000002 0.000001 0.000001 0.000001 0.000002 0.000001 0.000001 0.000002
Endoplasmic Reticulum 0.001962 0.001028 0.000367 0.000460 0.001227 0.001089 0.000922 0.000347 0.001467 0.001066 0.000714 0.000409
Endosome 0.001061 0.000796 0.000494 0.001121 0.000495 0.000622 0.000366 0.000635 0.000679 0.000688 0.000414 0.000901
Golgi 0.196429* 0.067377 0.066957 0.012792 0.078283 0.038608 0.020154 0.036298 0.116788* 0.049536 0.037702 0.023422
Lipid Particles 0.015640 0.015165 0.006581 0.005961 0.017332 0.006087 0.013945 0.007172 0.016780 0.009535 0.011184 0.006509
Mitochondria 0.000617 0.000283 0.000247 0.000846 0.000151 0.001212 0.000305 0.000333 0.000303 0.000859 0.000283 0.000614
Mitotic Spindle 0.001407 0.001709 0.001920 0.001289 0.000645 0.001468 0.000326 0.001218 0.000893 0.001560 0.000923 0.001257
None 0.002638 0.003123 0.003020 0.005783 0.002036 0.001975 0.000995 0.002049 0.002232 0.002411 0.001755 0.004094
Nuclear Periphery 0.001191 0.000800 0.001846 0.000310 0.001106 0.000259 0.000346 0.000310 0.001134 0.000464 0.000908 0.000310
Nuclear Periphery Foci 0.015134 0.015851 0.018905 0.004188 0.016373 0.005573 0.005098 0.005663 0.015969 0.009477 0.010275 0.004855
Nucleolus 0.000646 0.000129 0.000097 0.000400 0.000290 0.000045 0.000080 0.000084 0.000406 0.000077 0.000086 0.000257
Nucleus 0.007829 0.005889 0.018840 0.025065 0.005322 0.003193 0.004211 0.008492 0.006139 0.004217 0.009696 0.017571
Peroxisomes 0.000020 0.000024 0.000009 0.000215 0.000018 0.000140 0.000103 0.000047 0.000019 0.000096 0.000068 0.000139
Vacuole 0.093814 0.089509 0.080744 0.215599* 0.097640 0.040963 0.118585 0.089464 0.096393 0.059404 0.104398 0.158560
Vacuole Periphery 0.302429* 0.078078 0.112174* 0.055204 0.109281* 0.062882 0.044931 0.058311 0.172230* 0.068655 0.070142 0.056609

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.28 1.40 -0.02 -2.86 -3.25 -1.22 -3.11 -4.29 -2.84 -2.46 0.93 0.88 -1.59 -3.80 -3.86
Bud Neck -4.15 -6.58 -10.16 -7.77 -4.32 1.50 -1.02 -5.45 -6.32 -4.87 -2.35 -6.38 -11.18 -9.96 -7.04
Bud Site -1.31 0.32 -8.45 -7.81 -8.86 -5.19 -3.87 -9.08 -3.53 -6.84 -5.32 -2.87 -13.04 -7.56 -11.34
Cell Periphery 4.79 9.05 9.60 7.00 2.05 11.57 2.69 10.40 -1.36 10.77 11.97 7.16 14.52 3.95 11.66
Cytoplasm -23.44 -22.06 -21.37 4.87 2.37 -16.28 -11.13 -10.56 6.18 -0.23 -24.19 -19.68 -16.25 10.89 4.43
Cytoplasmic Foci 1.75 0.91 8.19 6.86 8.27 -5.23 3.78 -3.70 2.03 -7.89 -3.17 2.42 3.40 6.73 0.92
Eisosomes 1.67 1.30 -0.58 -3.25 -2.72 3.50 1.05 1.88 -1.97 1.11 3.08 1.53 -1.05 -4.32 -2.82
Endoplasmic Reticulum 2.62 4.68 4.53 4.52 -1.13 0.83 2.04 6.62 7.75 6.68 2.53 5.04 7.54 8.65 5.76
Endosome 2.26 5.27 -0.42 -2.27 -4.52 -2.33 3.60 -2.75 -0.18 -5.88 -0.28 6.14 -2.69 -2.55 -6.28
Golgi 17.48 17.91 26.74 20.53 23.95* 11.99 19.37 12.81 1.26 -8.47 19.15 23.52 28.49 16.30 11.42
Lipid Particles 0.10 5.60 6.39 7.81 1.88 9.33 2.37 7.98 -1.95 7.44 6.96 5.15 10.62 4.90 8.12
Mitochondria 1.88 2.08 -1.01 -4.93 -5.35 -2.13 -5.36 -3.99 1.76 -0.59 -1.77 0.29 -3.63 0.82 -5.13
Mitotic Spindle -1.10 -3.22 -1.15 -0.22 0.32 -1.99 4.74 -3.76 0.42 -5.66 -2.40 -0.89 -3.20 -0.01 -2.93
None -0.73 -1.26 -3.20 -2.24 -2.66 0.12 5.73 -0.03 -0.07 -1.85 -0.67 2.73 -3.12 -2.49 -3.96
Nuclear Periphery 1.55 -3.38 3.90 5.84 11.63 12.75 11.15 11.47 -2.85 0.91 7.65 1.58 9.52 3.42 10.88
Nuclear Periphery Foci -0.65 -2.67 8.47 11.49 13.19 13.05 13.73 12.75 -0.52 -1.38 8.25 6.83 15.83 9.21 10.56
Nucleolus 1.24 1.37 0.48 -4.70 -7.26 1.16 1.00 0.95 -8.35 -1.63 1.66 1.65 0.60 -6.53 -7.52
Nucleus 0.74 -6.17 -11.64 -15.48 -6.65 2.80 0.34 -5.27 -7.33 -5.59 2.03 -5.69 -14.68 -17.43 -10.85
Peroxisomes -0.67 1.88 -1.75 -1.70 -1.87 -1.16 -5.13 -4.04 0.89 3.32 -1.18 -4.62 -2.00 -0.30 -1.04
Vacuole 0.18 1.45 -17.45 -19.65 -21.94 12.61 -4.38 0.83 -12.50 5.54 9.51 -2.69 -14.29 -25.67 -13.46
Vacuole Periphery 22.21* 18.49 26.10* 8.29 14.33 9.70 14.65 10.76 1.73 -4.30 20.67 20.68 24.67 7.01 7.56
Standard name
Human Ortholog
Description Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Rps17b

Rps17b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps17b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available