Standard name
Human Ortholog
Description Transcription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.13 0.16 0.09 0.05 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0 0 0.05 0 0.11 0.09 0.05 0.07 0.11 0.15 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.9 0.92 0.87 0.88 0.89 0.8 0.74 0.7 0.67 0.64 0.65 0.6 0.88 0.82 0.78 0.86 0.86 0.81 0.9 0.79 0.74 0.83 0.78 0.69
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.28 0.29 0.25 0.2 0.23 0.36 0.39 0.43 0.52 0.54 0.56 0.55 0.06 0 0.07 0.07 0.13 0.08 0.06 0.13 0.17 0.1 0.12 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 1 1 1 7 1 3 6 3 2 0 0 0 0 0 2 1 3 5 1 3 4
Bud Neck 0 0 1 2 1 3 5 8 6 8 3 3 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 1 0 1 3 10 13 19 13 9 27 11 18 0 1 0 0 1 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 2 2 12 7 15 12 14 14 5 3 5 6 42 57 84 29 21 33 0 0 1 1 2 3
Endoplasmic Reticulum 1 0 0 0 0 0 0 1 0 0 0 0 0 1 1 2 7 5 0 0 0 0 0 1
Endosome 0 0 4 1 0 0 0 0 1 0 0 0 2 1 4 1 3 3 0 1 7 2 7 13
Golgi 0 1 1 0 0 1 0 0 0 0 0 0 0 0 0 2 2 4 0 1 2 1 0 2
Mitochondria 32 4 5 11 5 43 50 31 31 58 54 66 1 0 0 4 11 11 0 0 1 3 8 11
Nucleus 171 177 369 213 343 319 420 423 295 346 243 300 391 354 411 271 340 276 171 160 327 366 505 418
Nuclear Periphery 0 1 0 3 1 3 8 4 5 0 4 3 1 4 1 1 0 2 0 0 0 0 1 2
Nucleolus 54 55 106 49 87 143 220 261 229 292 208 276 26 17 39 23 52 27 11 26 74 43 77 92
Peroxisomes 0 0 1 0 0 0 0 0 0 1 0 0 0 1 1 0 2 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 2 1 3 4 5 0 1 2 5 8 13 5 3 2 0 3 5 2 5 12
Vac/Vac Membrane 0 2 0 3 1 1 1 5 1 1 0 1 3 2 4 2 4 6 3 3 14 16 31 36
Unique Cell Count 191 192 422 241 386 398 570 608 443 537 372 499 446 432 524 316 397 340 190 203 444 444 648 608
Labelled Cell Count 261 242 503 294 466 540 747 765 590 742 532 677 471 446 558 340 447 371 190 203 444 444 648 608


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.8 8.3 7.5 7.2 6.8 6.1 6.2 6.2 6.5 6.0 5.5 5.8 8.8 8.8 8.2 10.4 9.3 9.8 9.2 9.5 9.7
Std Deviation (1e-4) 1.0 1.9 1.7 1.9 2.0 2.1 1.7 1.5 1.6 1.6 1.6 1.6 1.9 2.0 2.0 2.5 2.0 2.2 1.6 1.6 1.7
Intensity Change (Log2) – – – -0.06 -0.15 -0.3 -0.27 -0.27 -0.2 -0.32 -0.43 -0.36 0.24 0.23 0.13 0.47 0.32 0.39 0.3 0.34 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 2.9 3.3 3.4 2.6 0 4.4 3.1 3.6 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.8 0.1 -0.4 -0.5 -1.8 -2.8 -1.5 -1.8 4.0 5.5 6.7 3.7 1.8 4.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.7 0 5.9 5.2 3.4 4.3 6.0 7.1 6.8 0 0 0 0 1.6 2.0
Nucleus 0.4 0.6 -2.8 -5.3 -6.7 -7.3 -8.1 -7.4 -9.3 0.1 -2.2 -3.6 -0.7 -0.8 -2.4
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.4 -0.9 3.4 4.5 5.9 8.0 9.1 8.9 9.3 -7.9 -8.8 -7.5 -6.3 -4.4 -6.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 2.5 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0126 5.3967 4.9951 4.5013 4.7416 4.7539 4.513 4.054 3.9506 3.7071 3.3201 3.9321 4.5222 4.3241 4.175 4.113 3.584 3.8007
Actin 0.0246 0 0 0 0.0002 0 0.0602 0 0.0033 0.0059 0.0168 0.0003 0.0342 0 0.0323 0.0234 0 0.0076
Bud 0.0002 0 0 0 0.0001 0 0.0008 0 0.0001 0.0002 0.0001 0.0001 0.0002 0 0.0003 0.0005 0.0001 0.0001
Bud Neck 0.0009 0 0 0 0.0014 0.0013 0.0071 0 0.0001 0.0015 0.0003 0.0005 0.0011 0 0.0015 0.0002 0.0003 0.0005
Bud Periphery 0.0002 0 0 0 0.0001 0.0001 0.0021 0 0.0001 0.0002 0.0003 0.0002 0.0003 0 0.0009 0.0006 0.0001 0.0001
Bud Site 0.0003 0 0 0 0.0002 0.0001 0.0095 0 0.0011 0.0009 0.0004 0.0001 0.0015 0 0.0014 0.0006 0.0001 0.0002
Cell Periphery 0.0001 0 0 0 0 0.0001 0.0005 0 0 0.0001 0.0001 0 0.0001 0 0.0004 0 0.0001 0
Cytoplasm 0.0009 0 0 0 0.0002 0 0.0223 0 0.0001 0.0006 0.0002 0.0001 0.0008 0 0.0003 0.0017 0.0005 0
Cytoplasmic Foci 0.0012 0 0 0 0.0001 0 0.026 0 0.0077 0.0211 0.0002 0.0009 0.0129 0 0.0003 0.002 0.0001 0.0003
Eisosomes 0.0001 0 0 0 0 0 0.0007 0 0 0 0.0003 0 0.0004 0 0.0003 0.0001 0 0.0001
Endoplasmic Reticulum 0.0013 0 0 0 0 0 0.0044 0 0.0001 0.0004 0.0001 0.0001 0.0009 0 0.0019 0.0023 0.0002 0.0003
Endosome 0.0035 0 0 0 0.0002 0.0002 0.0274 0 0.0025 0.0275 0.0004 0.0007 0.009 0 0.0011 0.0091 0.0005 0.0017
Golgi 0.0014 0 0 0 0.0001 0 0.0158 0 0.002 0.0156 0.0012 0.0004 0.0041 0 0.0009 0.0021 0 0.0015
Lipid Particles 0.0011 0 0 0 0.0001 0.0001 0.0227 0 0.006 0.0204 0.0006 0.0085 0.0046 0 0.0004 0.0016 0.0002 0.0011
Mitochondria 0.0009 0.0001 0.0001 0.0001 0.0004 0.0001 0.0403 0.0001 0.0045 0.0022 0.0198 0.0006 0.001 0.0001 0.0015 0.0026 0.0005 0.008
None 0.0023 0 0 0 0.0002 0 0.0148 0 0.0002 0.0002 0.0003 0.0001 0.0013 0 0.0003 0.0012 0.0003 0.0001
Nuclear Periphery 0.0082 0.0001 0.0002 0.0002 0.0021 0.0002 0.0258 0.0001 0.0002 0.0006 0.0039 0.0034 0.0018 0.0002 0.0031 0.022 0.0062 0.0007
Nucleolus 0.0233 0.0229 0.0238 0.009 0.1833 0.0937 0.0289 0.0202 0.0168 0.018 0.0911 0.0631 0.0292 0.0306 0.0154 0.0112 0.1969 0.0709
Nucleus 0.9255 0.9768 0.9756 0.9906 0.785 0.903 0.6517 0.9794 0.9404 0.8737 0.8613 0.9184 0.8787 0.9654 0.9347 0.9086 0.789 0.9059
Peroxisomes 0.0007 0 0 0 0.0001 0 0.015 0 0.0132 0.0023 0.0011 0.0001 0.0031 0 0.0002 0.0008 0 0.0002
Punctate Nuclear 0.0024 0.0001 0.0002 0.0001 0.0259 0.0006 0.0147 0.0001 0.0011 0.0071 0.0011 0.0019 0.0144 0.0036 0.0016 0.008 0.0021 0.0005
Vacuole 0.0004 0 0 0 0.0001 0.0004 0.0053 0 0.0003 0.0011 0.0002 0.0003 0.0002 0 0.0007 0.0006 0.0016 0.0001
Vacuole Periphery 0.0004 0 0 0 0.0001 0.0001 0.0043 0 0.0001 0.0004 0.0003 0.0003 0.0002 0 0.0005 0.0009 0.0014 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 19.8697 18.1403 17.9478 15.6863 23.0213 15.2109 21.0164 19.3427 18.0395 22.0265
Translational Efficiency 0.8026 0.9087 0.685 0.7012 0.8574 1.0427 0.673 0.7599 0.6839 0.7088

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1695 1002 2498 82 1588 1713 150 757 3283 2715 2648 839

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 865.55 954.58 1022.54 1186.06 820.29 916.26 1079.66 1483.76 843.66 930.40 1025.78 1454.66
Standard Deviation 121.65 154.60 159.77 201.39 129.24 142.30 145.55 341.65 127.40 148.12 159.55 342.20
Intensity Change Log 2 — 0.141249 0.240468 0.454488 — 0.159623 0.396371 0.855052 — 0.150219 0.318432 0.663275

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000298 0.000760 0.000591 0.001622 0.000386 0.000642 0.000932 0.000353 0.000341 0.000686 0.000610 0.000477
Bud Neck 0.005884 0.014329 0.019180 0.011490 0.005795 0.009013 0.004244 0.041399 0.005841 0.010975 0.018334 0.038476
Bud Site 0.000386 0.001290 0.001298 0.002262 0.000548 0.001028 0.000545 0.002384 0.000464 0.001124 0.001256 0.002372
Cell Periphery 0.000044 0.000068 0.000099 0.000251 0.000048 0.000056 0.000024 0.000434 0.000046 0.000060 0.000095 0.000417
Cytoplasm 0.000096 0.000916 0.001025 0.000612 0.000876 0.000397 0.000133 0.000705 0.000473 0.000589 0.000975 0.000696
Cytoplasmic Foci 0.000040 0.000210 0.000094 0.000048 0.000042 0.000333 0.000055 0.000008 0.000041 0.000287 0.000091 0.000012
Eisosomes 0.000052 0.000046 0.000059 0.000051 0.000042 0.000053 0.000056 0.000046 0.000047 0.000050 0.000059 0.000046
Endoplasmic Reticulum 0.002673 0.002711 0.001781 0.004027 0.002434 0.002699 0.003477 0.002820 0.002557 0.002704 0.001877 0.002938
Endosome 0.000146 0.000343 0.000121 0.000171 0.000103 0.000840 0.000057 0.000208 0.000125 0.000657 0.000117 0.000205
Golgi 0.000061 0.000327 0.000082 0.000143 0.000073 0.000332 0.000074 0.000056 0.000067 0.000330 0.000082 0.000064
Lipid Particles 0.000047 0.000170 0.000031 0.000096 0.000045 0.000512 0.000112 0.000007 0.000046 0.000386 0.000036 0.000016
Mitochondria 0.000985 0.002485 0.001157 0.004364 0.000681 0.001865 0.000741 0.001302 0.000838 0.002094 0.001134 0.001601
Mitotic Spindle 0.002017 0.002917 0.002252 0.011895 0.001764 0.005640 0.000071 0.003638 0.001895 0.004635 0.002129 0.004445
None 0.001184 0.001376 0.002866 0.001534 0.002065 0.001417 0.001097 0.001033 0.001610 0.001402 0.002766 0.001082
Nuclear Periphery 0.000181 0.000188 0.000164 0.000387 0.000119 0.000356 0.000085 0.000808 0.000151 0.000294 0.000160 0.000767
Nuclear Periphery Foci 0.000319 0.000456 0.000082 0.000821 0.000138 0.000876 0.000035 0.000105 0.000232 0.000721 0.000079 0.000175
Nucleolus 0.072091 0.070780 0.044345 0.040455 0.072368 0.078811 0.033292 0.020598 0.072225 0.075847 0.043719 0.022539
Nucleus 0.912542* 0.898373* 0.923741* 0.917031* 0.911433* 0.892874* 0.952968* 0.921033* 0.912006* 0.894904* 0.925396* 0.920642*
Peroxisomes 0.000511 0.001126 0.000563 0.001000 0.000541 0.001102 0.001150 0.000045 0.000525 0.001111 0.000596 0.000138
Vacuole 0.000205 0.000615 0.000295 0.001482 0.000188 0.000625 0.000767 0.002464 0.000197 0.000622 0.000321 0.002368
Vacuole Periphery 0.000237 0.000512 0.000173 0.000257 0.000312 0.000529 0.000084 0.000554 0.000273 0.000523 0.000168 0.000525

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.98 -10.44 -6.10 -3.83 -4.61 -4.99 -6.22 -0.80 4.47 5.99 -7.35 -12.22 -5.12 2.59 3.26
Bud Neck -7.59 -18.74 -2.88 0.20 2.11 -5.03 1.73 -12.35 -11.29 -12.63 -9.03 -19.26 -12.52 -10.61 -7.80
Bud Site -3.47 -9.17 -2.29 -1.21 -1.15 -2.52 -1.28 -4.10 -2.99 -3.80 -4.39 -8.46 -4.66 -3.04 -2.62
Cell Periphery -1.81 -3.50 -1.19 -1.04 -0.90 -0.83 2.96 -2.15 -2.09 -2.29 -1.82 -3.56 -2.30 -2.18 -1.96
Cytoplasm -1.95 -3.97 -1.23 0.27 0.45 0.59 1.31 0.14 -0.88 -4.95 -0.50 -1.37 -1.08 -0.49 0.60
Cytoplasmic Foci -1.83 -1.18 -0.53 1.69 0.75 -3.12 -0.61 1.64 3.41 2.32 -3.60 -1.25 1.62 3.91 1.88
Eisosomes 1.21 -2.16 -0.31 -1.13 1.02 -4.78 -4.16 0.03 1.27 2.06 -1.62 -5.35 0.51 0.99 2.12
Endoplasmic Reticulum -0.23 3.39 -1.55 -1.49 -2.70 -1.64 -3.48 -0.49 0.43 2.86 -1.02 3.58 -0.51 0.05 -2.58
Endosome -1.75 0.42 -0.54 1.33 -1.04 -2.58 1.26 -1.94 2.23 -2.48 -2.88 0.17 -1.39 2.48 -1.75
Golgi -2.13 -2.71 -2.65 1.10 -2.09 -2.69 -1.31 -1.34 2.54 0.06 -3.45 -3.34 -2.85 3.06 -0.53
Lipid Particles -1.97 0.73 -1.48 1.17 -2.15 -1.93 -3.13 2.84 2.08 4.67 -2.26 0.57 2.25 2.44 2.72
Mitochondria -1.58 -0.89 -1.91 -0.86 -1.77 -3.04 -1.29 -4.43 0.73 -3.96 -2.88 -2.06 -3.59 0.62 -2.27
Mitotic Spindle -0.51 -0.32 -1.37 -1.30 -1.34 -2.43 1.95 -1.93 -0.17 -2.91 -2.33 -0.44 -2.35 -1.10 -2.15
None -0.36 -2.80 -0.66 -0.49 1.28 1.53 2.63 2.33 0.58 -0.96 0.57 -2.50 0.91 0.39 3.85
Nuclear Periphery -0.19 0.05 -2.19 -2.20 -2.34 -2.44 -0.32 -3.34 -2.01 -3.24 -2.22 -0.88 -3.33 -2.46 -3.22
Nuclear Periphery Foci -0.78 1.65 -0.80 -0.46 -1.13 -2.77 1.95 -0.43 2.66 -2.09 -2.48 1.93 -0.03 2.42 -1.79
Nucleolus 0.38 8.19 0.96 0.85 -0.74 -1.95 6.96 7.08 8.26 -0.63 -1.46 10.36 7.32 7.92 1.63
Nucleus 3.05 -1.47 1.11 0.41 1.35 4.29 -6.60 2.29 -0.34 6.47 5.34 -2.57 2.64 -0.02 3.71
Peroxisomes -1.68 -0.61 -2.83 0.18 -2.51 -4.47 -3.97 14.26 8.19 6.84 -3.33 -1.04 5.51 5.09 4.64
Vacuole -1.90 -1.93 -1.95 -1.23 -1.65 -3.34 -3.57 -2.33 -1.75 -1.03 -3.73 -2.70 -2.48 -1.87 -2.15
Vacuole Periphery -1.39 1.22 -0.41 1.15 -1.05 -1.51 1.94 -0.67 0.19 -1.62 -2.29 1.46 -0.87 0.25 -1.32
Standard name
Human Ortholog
Description Transcription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Ada2

Ada2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ada2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available