Standard name
Human Ortholog
Description Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.72 0.86 0.87 0.83 0.85 0.73 0.72 0.7 0.66 0.6 0.69 0.9 0.99 1.0 0.86 0.78 0.78 0.56 0.47 0.56 0.89 0.83 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.07 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0 0 0.1 0.37 0.22 0.37 0.35 0.5 0.26 0 0 0 0 0 0 0.06 0.05 0.07 0.05 0.05 0
Nucleus 0.11 0.22 0.12 0.09 0.11 0.06 0.1 0.17 0.09 0.12 0.11 0.15 0 0 0 0.06 0.12 0.12 0.12 0.22 0.14 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.07 0.08 0 0 0
Vac/Vac Membrane 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.11 0.1 0.05 0.07 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 2 4 1 0 2 3
Bud 0 1 0 1 1 2 5 4 5 8 3 4 0 0 0 0 0 1 8 21 4 0 4 2
Bud Neck 0 1 3 2 5 3 2 3 2 8 5 9 0 1 0 0 0 0 2 2 2 0 1 3
Bud Site 0 0 0 0 0 0 1 1 2 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 1 0 2 1 0 1 0 0 1 1 0 1 0 0 5 9 5 0 0 2 0 1 1
Cytoplasm 151 217 185 160 189 133 132 130 188 173 121 146 102 202 123 234 256 215 113 148 119 150 184 228
Endoplasmic Reticulum 0 2 0 1 0 0 0 0 0 1 0 0 1 0 1 24 17 19 1 0 1 0 1 1
Endosome 0 0 1 1 3 0 0 0 0 0 0 0 0 1 0 2 2 2 3 3 0 0 1 1
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 2 0 0 3 0 0 0 0
Mitochondria 32 6 0 0 4 16 67 39 100 93 100 55 5 0 0 1 9 6 11 16 14 8 11 11
Nucleus 22 67 27 16 25 9 18 31 24 32 23 31 5 2 0 16 38 33 24 70 30 0 1 0
Nuclear Periphery 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 2 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 3 3 1 3 2 5 1 0 2 0 0 0 1 2 0 13 22 16 1 3 3
Vac/Vac Membrane 5 16 1 4 3 5 1 0 2 1 0 0 1 3 0 20 35 27 10 20 9 0 4 2
Unique Cell Count 194 302 216 183 227 157 182 181 270 262 202 212 113 205 123 273 327 276 201 320 213 170 222 266
Labelled Cell Count 212 311 220 190 234 170 230 211 328 318 254 249 117 209 124 304 370 308 201 320 213 170 222 266


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.0 4.5 4.4 4.3 4.4 3.5 3.0 3.7 2.8 3.3 2.7 3.2 4.1 5.0 4.8 5.5 5.4 5.1 4.0 4.4 4.9
Std Deviation (1e-4) 0.6 1.1 1.2 1.8 1.5 1.5 1.1 1.6 0.6 1.9 0.6 1.0 1.1 1.7 1.7 1.2 1.5 1.2 1.1 1.4 1.7
Intensity Change (Log2) – – – -0.04 0.0 -0.35 -0.54 -0.24 -0.64 -0.43 -0.72 -0.45 -0.12 0.17 0.13 0.31 0.29 0.2 -0.15 -0.02 0.14


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 3.7 4.7 4.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8098 3.7126 3.1386 3.1969 0.447 2.2354 -0.8114 -0.331 0.5038 0.0593 0.0836 -0.6336 -0.8442 -0.214 -0.5449 -0.3716 1.6053 -1.7436
Actin 0.0166 0.0001 0.007 0.0001 0.0026 0.003 0.0227 0.0006 0.0069 0.0001 0.0217 0.0024 0.0098 0.0006 0.0062 0.0103 0.0085 0.0003
Bud 0.0035 0.0028 0.0016 0.0012 0.0014 0.0018 0.0059 0.0017 0.0008 0.0002 0.0008 0.002 0.0063 0.0045 0.0018 0.0008 0.0196 0.0012
Bud Neck 0.001 0.0008 0.0015 0.0004 0.0003 0.003 0.0104 0.0008 0.0014 0.0004 0.0013 0.0014 0.0011 0.0003 0.0004 0.0038 0.0352 0.0034
Bud Periphery 0.003 0.0012 0.0033 0.0011 0.0009 0.0027 0.0125 0.0016 0.0009 0.0001 0.0021 0.0045 0.0095 0.0027 0.0026 0.0008 0.0337 0.0022
Bud Site 0.0029 0.0124 0.0055 0 0.0002 0.0007 0.0147 0.005 0.0049 0.0001 0.0013 0.0003 0.0055 0.0047 0.0015 0.0034 0.0069 0.0003
Cell Periphery 0.0006 0.0003 0.0004 0.0001 0.0001 0.0002 0.0018 0.0003 0.0002 0 0.0002 0.0002 0.0011 0.0002 0.0002 0.0001 0.0016 0.0002
Cytoplasm 0.0195 0.0351 0.0096 0.0371 0.0904 0.0522 0.0465 0.071 0.0359 0.0231 0.0578 0.0445 0.0796 0.1251 0.0652 0.0509 0.245 0.111
Cytoplasmic Foci 0.0434 0.0025 0.0118 0.0008 0.0337 0.0115 0.0192 0.0022 0.0073 0.0059 0.0276 0.0042 0.0265 0.0081 0.0053 0.075 0.0347 0.0061
Eisosomes 0.0003 0 0.0001 0 0 0 0.0005 0.0001 0.0001 0 0.0009 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0018 0.0006 0.0022 0.0011 0.0017 0.0014 0.0044 0.0016 0.0019 0.0003 0.002 0.0023 0.0073 0.0015 0.0014 0.0012 0.0056 0.0015
Endosome 0.0168 0.0004 0.0088 0.0005 0.0093 0.0029 0.0117 0.0013 0.0153 0.0006 0.0279 0.0087 0.0119 0.0031 0.0079 0.0087 0.018 0.0014
Golgi 0.0057 0 0.0047 0 0.0156 0.0008 0.0042 0.0001 0.0028 0 0.0142 0.001 0.005 0.0009 0.004 0.0158 0.0172 0.0001
Lipid Particles 0.0184 0.0002 0.0046 0.0001 0.0079 0.0009 0.0097 0.0009 0.0049 0.0001 0.0436 0.0009 0.0206 0.0061 0.0062 0.0086 0.0098 0.0003
Mitochondria 0.0178 0.0005 0.0235 0.0002 0.0011 0.0007 0.0103 0.0016 0.0019 0.0003 0.0192 0.0015 0.0258 0.0017 0.005 0.0376 0.0101 0.0005
None 0.2807 0.1305 0.0606 0.1855 0.6125 0.356 0.6235 0.4599 0.1581 0.7481 0.6213 0.7717 0.6556 0.5633 0.1289 0.3563 0.3212 0.7878
Nuclear Periphery 0.009 0.0029 0.0119 0.0021 0.0034 0.006 0.0119 0.0075 0.0114 0.0012 0.0145 0.0102 0.0156 0.0075 0.0085 0.0042 0.0075 0.0029
Nucleolus 0.014 0.0119 0.0086 0.0041 0.0016 0.0194 0.015 0.0109 0.0087 0.0024 0.0059 0.0041 0.0072 0.0027 0.0054 0.0014 0.0022 0.0023
Nucleus 0.5048 0.7803 0.8093 0.7628 0.171 0.5087 0.1286 0.3973 0.7104 0.1645 0.0671 0.1276 0.0612 0.2478 0.7286 0.4047 0.1315 0.0715
Peroxisomes 0.0169 0.0001 0.0047 0 0.0301 0.0002 0.0173 0.0002 0.002 0.0001 0.0408 0.0004 0.0139 0.0035 0.0097 0.0116 0.0754 0.0001
Punctate Nuclear 0.0156 0.0154 0.016 0.0016 0.0133 0.0237 0.0076 0.0331 0.0197 0.0521 0.0219 0.0092 0.0141 0.0129 0.0069 0.0034 0.0036 0.0044
Vacuole 0.0064 0.0015 0.0032 0.001 0.0021 0.0031 0.0182 0.0018 0.0034 0.0003 0.0061 0.002 0.0182 0.0023 0.0032 0.0011 0.0113 0.0019
Vacuole Periphery 0.0013 0.0003 0.0012 0.0002 0.0007 0.0008 0.0034 0.0004 0.001 0.0001 0.0019 0.0008 0.0038 0.0005 0.0009 0.0004 0.0014 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.8704 42.7244 7.5812 8.3543 20.2064 7.2689 36.8597 25.0362 15.881 22.2056
Translational Efficiency 2.2829 2.5338 2.7605 2.0061 2.7279 3.4981 1.7422 2.176 1.9419 2.0284

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1646 924 1752 229 1311 1771 181 1815 2957 2695 1933 2044

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 667.04 661.85 809.92 939.27 657.32 706.96 887.29 868.92 662.73 691.49 817.16 876.80
Standard Deviation 82.48 103.65 100.67 126.70 81.11 97.01 91.87 127.24 82.01 101.62 102.39 129.10
Intensity Change Log 2 — -0.011269 0.280006 0.493767 — 0.105033 0.432810 0.402627 — 0.047627 0.357870 0.449251

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000208 0.000529 0.000172 0.000747 0.000061 0.000364 0.000285 0.001167 0.000143 0.000420 0.000182 0.001120
Bud Neck 0.024176 0.030021 0.004797 0.005368 0.024296 0.036321 0.001793 0.012513 0.024229 0.034161 0.004516 0.011712
Bud Site 0.005156 0.015405 0.003349 0.009737 0.008401 0.023272 0.001306 0.042640 0.006594 0.020575 0.003157 0.038954
Cell Periphery 0.000504 0.000372 0.000154 0.000243 0.000520 0.000459 0.000222 0.000219 0.000511 0.000429 0.000160 0.000222
Cytoplasm 0.343900* 0.309212* 0.253466* 0.387280* 0.448062* 0.357956* 0.208293* 0.466112* 0.390081* 0.341244* 0.249236* 0.457280*
Cytoplasmic Foci 0.194651* 0.179471 0.006555 0.010413 0.063594 0.138195 0.025272 0.010749 0.136546 0.152347 0.008308 0.010711
Eisosomes 0.000210 0.000138 0.000027 0.000059 0.000078 0.000135 0.000095 0.000028 0.000152 0.000136 0.000034 0.000032
Endoplasmic Reticulum 0.001096 0.002769 0.002041 0.007599 0.001441 0.001482 0.003939 0.002100 0.001249 0.001924 0.002219 0.002716
Endosome 0.010086 0.020341 0.001433 0.002638 0.003156 0.016363 0.003377 0.004775 0.007014 0.017727 0.001615 0.004536
Golgi 0.003143 0.005170 0.000062 0.000108 0.000710 0.004099 0.000083 0.002452 0.002064 0.004466 0.000064 0.002189
Lipid Particles 0.013495 0.009552 0.000893 0.001870 0.003502 0.006678 0.003180 0.000565 0.009065 0.007663 0.001107 0.000711
Mitochondria 0.011072 0.018216 0.000747 0.000915 0.002626 0.013666 0.000381 0.006330 0.007327 0.015226 0.000713 0.005723
Mitotic Spindle 0.003120 0.003171 0.006001 0.014138 0.000325 0.009071 0.000385 0.070424 0.001881 0.007048 0.005475 0.064118
None 0.020849 0.024298 0.008223 0.015792 0.020135 0.013204 0.006212 0.010466 0.020532 0.017007 0.008035 0.011063
Nuclear Periphery 0.000634 0.001111 0.001638 0.001820 0.000639 0.000605 0.001676 0.001281 0.000636 0.000779 0.001641 0.001342
Nuclear Periphery Foci 0.000487 0.000706 0.000341 0.003393 0.000314 0.000951 0.001181 0.000592 0.000410 0.000867 0.000420 0.000906
Nucleolus 0.004955 0.008478 0.000889 0.000599 0.002111 0.006834 0.001632 0.001123 0.003694 0.007397 0.000958 0.001065
Nucleus 0.328402* 0.308847* 0.690893* 0.419883* 0.396891* 0.322417* 0.693451* 0.292122* 0.358767* 0.317764* 0.691133* 0.306435*
Peroxisomes 0.012752 0.021292 0.000169 0.000299 0.001155 0.010099 0.000661 0.001561 0.007611 0.013936 0.000215 0.001420
Vacuole 0.020099 0.038095 0.017902 0.116917 0.021448 0.035369 0.046397 0.069643 0.020697 0.036304 0.020571 0.074939
Vacuole Periphery 0.001007 0.002805 0.000247 0.000183 0.000533 0.002460 0.000178 0.003137 0.000796 0.002578 0.000241 0.002806

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.12 -1.41 -6.77 -3.52 -6.54 -6.50 -6.02 -4.03 -1.98 -1.01 -7.02 -5.85 -4.14 -2.04 -3.16
Bud Neck -2.00 10.41 8.07 8.43 -1.56 -4.07 10.31 5.91 11.44 -10.06 -4.80 13.57 8.93 13.48 -6.69
Bud Site -6.54 2.26 -1.61 3.51 -2.57 -6.35 5.70 -5.94 0.36 -9.45 -8.95 4.43 -7.24 0.01 -8.95
Cell Periphery 2.51 8.71 6.97 4.13 -2.62 1.06 5.55 7.75 8.12 4.18 2.07 11.22 11.08 9.38 0.84
Cytoplasm 3.07 13.54 6.65 4.35 -0.35 8.65 14.29 8.41 -0.47 -9.55 6.45 22.55 5.33 -0.73 -15.27
Cytoplasmic Foci 1.59 32.81 31.82 23.87 -1.72 -13.25 7.65 16.94 28.08 3.71 -2.97 34.36 34.46 36.75 -0.09
Eisosomes 4.33 12.64 8.33 5.95 -9.40 -3.60 -3.73 6.41 6.27 7.78 1.05 13.08 12.66 8.88 -2.03
Endoplasmic Reticulum -4.49 -12.50 -10.01 -7.48 -7.39 -0.23 -6.23 -7.25 -8.20 3.73 -3.51 -12.20 -12.98 -8.94 -2.54
Endosome -5.39 9.85 8.63 10.47 -3.19 -11.62 -2.07 1.27 12.49 3.16 -10.21 9.79 7.95 16.07 -2.27
Golgi -2.58 9.18 9.03 7.22 -4.24 -5.91 4.42 -0.42 4.78 -2.04 -4.97 10.11 3.28 6.65 -2.16
Lipid Particles 2.73 10.80 9.78 8.66 -5.42 -4.68 -0.52 6.26 10.86 7.52 1.68 11.44 11.99 14.60 4.01
Mitochondria -2.62 7.43 7.43 7.69 0.17 -7.49 4.80 -0.66 6.83 -4.24 -5.40 8.20 4.57 9.26 -3.72
Mitotic Spindle -0.24 -2.25 -1.41 -1.34 -0.46 -6.06 -1.29 -12.45 -9.14 -12.37 -4.70 -3.26 -11.92 -9.90 -9.89
None -1.07 8.03 7.39 6.55 0.23 3.36 6.96 6.47 4.49 -2.11 2.29 10.22 9.95 7.76 -1.40
Nuclear Periphery -1.20 -17.85 -11.34 -3.75 -2.77 0.33 -9.46 -15.73 -19.41 -1.67 -1.01 -19.21 -20.25 -11.24 -3.66
Nuclear Periphery Foci -1.82 0.31 -5.30 -4.86 -5.42 -1.76 -4.77 -5.75 0.61 3.60 -1.85 -2.71 -6.15 -0.51 -3.91
Nucleolus -3.72 9.43 9.86 8.76 2.28 -6.79 0.93 5.02 8.69 2.39 -6.28 10.30 10.11 11.96 1.00
Nucleus 1.39 -36.33 -5.20 -5.77 12.12 6.12 -11.95 0.05 -6.58 12.24 4.41 -40.43 -4.73 -8.72 33.15
Peroxisomes -4.18 12.63 12.54 11.07 -1.79 -12.30 1.04 2.64 13.01 0.43 -6.53 12.98 12.08 16.24 -2.53
Vacuole -6.20 -3.44 -12.70 -11.68 -12.33 -5.85 -6.44 -22.52 -19.35 -4.10 -8.94 -5.77 -26.19 -22.24 -22.65
Vacuole Periphery -5.17 7.84 9.08 7.91 1.51 -6.80 5.01 -2.07 3.05 -3.18 -8.00 8.27 -0.98 4.27 -3.03
Standard name
Human Ortholog
Description Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication
Localization
Cell Percentages nucleus (28%), cytoplasm (15%), mixed (30%)
Cell Cycle Regulation Yes
cytoplasm - MA (0.00014)
nucleus - S/G2 (8.1e-08)
Subcompartmental Group N/A

Yhp1

Yhp1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yhp1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available