Standard name | |
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Human Ortholog | |
Description | Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 1 | 0 | 2 | 3 |
Bud | 0 | 1 | 0 | 1 | 1 | 2 | 5 | 4 | 5 | 8 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 8 | 21 | 4 | 0 | 4 | 2 |
Bud Neck | 0 | 1 | 3 | 2 | 5 | 3 | 2 | 3 | 2 | 8 | 5 | 9 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 2 | 0 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 5 | 9 | 5 | 0 | 0 | 2 | 0 | 1 | 1 |
Cytoplasm | 151 | 217 | 185 | 160 | 189 | 133 | 132 | 130 | 188 | 173 | 121 | 146 | 102 | 202 | 123 | 234 | 256 | 215 | 113 | 148 | 119 | 150 | 184 | 228 |
Endoplasmic Reticulum | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 24 | 17 | 19 | 1 | 0 | 1 | 0 | 1 | 1 |
Endosome | 0 | 0 | 1 | 1 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 | 2 | 3 | 3 | 0 | 0 | 1 | 1 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
Mitochondria | 32 | 6 | 0 | 0 | 4 | 16 | 67 | 39 | 100 | 93 | 100 | 55 | 5 | 0 | 0 | 1 | 9 | 6 | 11 | 16 | 14 | 8 | 11 | 11 |
Nucleus | 22 | 67 | 27 | 16 | 25 | 9 | 18 | 31 | 24 | 32 | 23 | 31 | 5 | 2 | 0 | 16 | 38 | 33 | 24 | 70 | 30 | 0 | 1 | 0 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 1 | 3 | 3 | 1 | 3 | 2 | 5 | 1 | 0 | 2 | 0 | 0 | 0 | 1 | 2 | 0 | 13 | 22 | 16 | 1 | 3 | 3 |
Vac/Vac Membrane | 5 | 16 | 1 | 4 | 3 | 5 | 1 | 0 | 2 | 1 | 0 | 0 | 1 | 3 | 0 | 20 | 35 | 27 | 10 | 20 | 9 | 0 | 4 | 2 |
Unique Cell Count | 194 | 302 | 216 | 183 | 227 | 157 | 182 | 181 | 270 | 262 | 202 | 212 | 113 | 205 | 123 | 273 | 327 | 276 | 201 | 320 | 213 | 170 | 222 | 266 |
Labelled Cell Count | 212 | 311 | 220 | 190 | 234 | 170 | 230 | 211 | 328 | 318 | 254 | 249 | 117 | 209 | 124 | 304 | 370 | 308 | 201 | 320 | 213 | 170 | 222 | 266 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.0 | 4.5 | 4.4 | 4.3 | 4.4 | 3.5 | 3.0 | 3.7 | 2.8 | 3.3 | 2.7 | 3.2 | 4.1 | 5.0 | 4.8 | 5.5 | 5.4 | 5.1 | 4.0 | 4.4 | 4.9 |
Std Deviation (1e-4) | 0.6 | 1.1 | 1.2 | 1.8 | 1.5 | 1.5 | 1.1 | 1.6 | 0.6 | 1.9 | 0.6 | 1.0 | 1.1 | 1.7 | 1.7 | 1.2 | 1.5 | 1.2 | 1.1 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.0 | -0.35 | -0.54 | -0.24 | -0.64 | -0.43 | -0.72 | -0.45 | -0.12 | 0.17 | 0.13 | 0.31 | 0.29 | 0.2 | -0.15 | -0.02 | 0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.7 | 4.7 | 4.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.8098 | 3.7126 | 3.1386 | 3.1969 | 0.447 | 2.2354 | -0.8114 | -0.331 | 0.5038 | 0.0593 | 0.0836 | -0.6336 | -0.8442 | -0.214 | -0.5449 | -0.3716 | 1.6053 | -1.7436 |
Actin | 0.0166 | 0.0001 | 0.007 | 0.0001 | 0.0026 | 0.003 | 0.0227 | 0.0006 | 0.0069 | 0.0001 | 0.0217 | 0.0024 | 0.0098 | 0.0006 | 0.0062 | 0.0103 | 0.0085 | 0.0003 |
Bud | 0.0035 | 0.0028 | 0.0016 | 0.0012 | 0.0014 | 0.0018 | 0.0059 | 0.0017 | 0.0008 | 0.0002 | 0.0008 | 0.002 | 0.0063 | 0.0045 | 0.0018 | 0.0008 | 0.0196 | 0.0012 |
Bud Neck | 0.001 | 0.0008 | 0.0015 | 0.0004 | 0.0003 | 0.003 | 0.0104 | 0.0008 | 0.0014 | 0.0004 | 0.0013 | 0.0014 | 0.0011 | 0.0003 | 0.0004 | 0.0038 | 0.0352 | 0.0034 |
Bud Periphery | 0.003 | 0.0012 | 0.0033 | 0.0011 | 0.0009 | 0.0027 | 0.0125 | 0.0016 | 0.0009 | 0.0001 | 0.0021 | 0.0045 | 0.0095 | 0.0027 | 0.0026 | 0.0008 | 0.0337 | 0.0022 |
Bud Site | 0.0029 | 0.0124 | 0.0055 | 0 | 0.0002 | 0.0007 | 0.0147 | 0.005 | 0.0049 | 0.0001 | 0.0013 | 0.0003 | 0.0055 | 0.0047 | 0.0015 | 0.0034 | 0.0069 | 0.0003 |
Cell Periphery | 0.0006 | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0002 | 0.0018 | 0.0003 | 0.0002 | 0 | 0.0002 | 0.0002 | 0.0011 | 0.0002 | 0.0002 | 0.0001 | 0.0016 | 0.0002 |
Cytoplasm | 0.0195 | 0.0351 | 0.0096 | 0.0371 | 0.0904 | 0.0522 | 0.0465 | 0.071 | 0.0359 | 0.0231 | 0.0578 | 0.0445 | 0.0796 | 0.1251 | 0.0652 | 0.0509 | 0.245 | 0.111 |
Cytoplasmic Foci | 0.0434 | 0.0025 | 0.0118 | 0.0008 | 0.0337 | 0.0115 | 0.0192 | 0.0022 | 0.0073 | 0.0059 | 0.0276 | 0.0042 | 0.0265 | 0.0081 | 0.0053 | 0.075 | 0.0347 | 0.0061 |
Eisosomes | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0009 | 0.0001 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0018 | 0.0006 | 0.0022 | 0.0011 | 0.0017 | 0.0014 | 0.0044 | 0.0016 | 0.0019 | 0.0003 | 0.002 | 0.0023 | 0.0073 | 0.0015 | 0.0014 | 0.0012 | 0.0056 | 0.0015 |
Endosome | 0.0168 | 0.0004 | 0.0088 | 0.0005 | 0.0093 | 0.0029 | 0.0117 | 0.0013 | 0.0153 | 0.0006 | 0.0279 | 0.0087 | 0.0119 | 0.0031 | 0.0079 | 0.0087 | 0.018 | 0.0014 |
Golgi | 0.0057 | 0 | 0.0047 | 0 | 0.0156 | 0.0008 | 0.0042 | 0.0001 | 0.0028 | 0 | 0.0142 | 0.001 | 0.005 | 0.0009 | 0.004 | 0.0158 | 0.0172 | 0.0001 |
Lipid Particles | 0.0184 | 0.0002 | 0.0046 | 0.0001 | 0.0079 | 0.0009 | 0.0097 | 0.0009 | 0.0049 | 0.0001 | 0.0436 | 0.0009 | 0.0206 | 0.0061 | 0.0062 | 0.0086 | 0.0098 | 0.0003 |
Mitochondria | 0.0178 | 0.0005 | 0.0235 | 0.0002 | 0.0011 | 0.0007 | 0.0103 | 0.0016 | 0.0019 | 0.0003 | 0.0192 | 0.0015 | 0.0258 | 0.0017 | 0.005 | 0.0376 | 0.0101 | 0.0005 |
None | 0.2807 | 0.1305 | 0.0606 | 0.1855 | 0.6125 | 0.356 | 0.6235 | 0.4599 | 0.1581 | 0.7481 | 0.6213 | 0.7717 | 0.6556 | 0.5633 | 0.1289 | 0.3563 | 0.3212 | 0.7878 |
Nuclear Periphery | 0.009 | 0.0029 | 0.0119 | 0.0021 | 0.0034 | 0.006 | 0.0119 | 0.0075 | 0.0114 | 0.0012 | 0.0145 | 0.0102 | 0.0156 | 0.0075 | 0.0085 | 0.0042 | 0.0075 | 0.0029 |
Nucleolus | 0.014 | 0.0119 | 0.0086 | 0.0041 | 0.0016 | 0.0194 | 0.015 | 0.0109 | 0.0087 | 0.0024 | 0.0059 | 0.0041 | 0.0072 | 0.0027 | 0.0054 | 0.0014 | 0.0022 | 0.0023 |
Nucleus | 0.5048 | 0.7803 | 0.8093 | 0.7628 | 0.171 | 0.5087 | 0.1286 | 0.3973 | 0.7104 | 0.1645 | 0.0671 | 0.1276 | 0.0612 | 0.2478 | 0.7286 | 0.4047 | 0.1315 | 0.0715 |
Peroxisomes | 0.0169 | 0.0001 | 0.0047 | 0 | 0.0301 | 0.0002 | 0.0173 | 0.0002 | 0.002 | 0.0001 | 0.0408 | 0.0004 | 0.0139 | 0.0035 | 0.0097 | 0.0116 | 0.0754 | 0.0001 |
Punctate Nuclear | 0.0156 | 0.0154 | 0.016 | 0.0016 | 0.0133 | 0.0237 | 0.0076 | 0.0331 | 0.0197 | 0.0521 | 0.0219 | 0.0092 | 0.0141 | 0.0129 | 0.0069 | 0.0034 | 0.0036 | 0.0044 |
Vacuole | 0.0064 | 0.0015 | 0.0032 | 0.001 | 0.0021 | 0.0031 | 0.0182 | 0.0018 | 0.0034 | 0.0003 | 0.0061 | 0.002 | 0.0182 | 0.0023 | 0.0032 | 0.0011 | 0.0113 | 0.0019 |
Vacuole Periphery | 0.0013 | 0.0003 | 0.0012 | 0.0002 | 0.0007 | 0.0008 | 0.0034 | 0.0004 | 0.001 | 0.0001 | 0.0019 | 0.0008 | 0.0038 | 0.0005 | 0.0009 | 0.0004 | 0.0014 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.8704 | 42.7244 | 7.5812 | 8.3543 | 20.2064 | 7.2689 | 36.8597 | 25.0362 | 15.881 | 22.2056 |
Translational Efficiency | 2.2829 | 2.5338 | 2.7605 | 2.0061 | 2.7279 | 3.4981 | 1.7422 | 2.176 | 1.9419 | 2.0284 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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