Standard name
Human Ortholog
Description Guanylate kinase; converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.98 0.96 0.95 0.9 – 0.87 0.81 0.86 0.81 0.99 0.99 0.99 0.96 0.96 0.93 0.97 0.95 0.96 0.97 0.97 0.95
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0.05 0.12 0.08 0.07 0 0 0 0 0.06 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 0 – 4 5 2 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 1 0 – 0 0 0 0 0 0 0 0 0 0 3 1 0 2 4 8
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 5 0 3 5 3 13 – 7 11 14 9 3 3 8 9 4 0 0 0 0 0 0 0
Cytoplasm 576 455 141 239 279 218 439 – 192 272 255 190 692 1066 1145 186 159 143 556 424 144 439 1024 679
Endoplasmic Reticulum 3 2 0 5 5 2 22 – 6 14 5 10 6 8 7 3 2 7 1 1 0 1 6 4
Endosome 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 1
Golgi 3 0 0 0 0 0 0 – 2 2 1 2 1 1 0 2 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 5 1 – 7 4 6 8 0 2 0 0 1 1 0 1 0 0 0 0
Nucleus 1 0 0 2 3 5 13 – 1 4 9 11 0 2 1 0 0 1 0 8 0 0 4 4
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
Vac/Vac Membrane 1 2 0 2 8 5 19 – 10 39 25 17 1 3 2 7 10 8 2 2 0 2 3 3
Unique Cell Count 581 459 141 245 292 229 489 220 334 298 235 698 1077 1151 193 166 154 573 446 151 452 1052 712
Labelled Cell Count 588 464 142 251 301 240 507 229 351 317 250 703 1085 1163 207 177 161 573 446 151 452 1052 712


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.4 29.4 29.0 25.4 23.6 14.6 19.7 – 17.0 17.1 16.6 16.2 35.3 34.5 33.8 35.5 39.1 32.7 31.1 33.0 34.9
Std Deviation (1e-4) 8.0 6.0 5.4 5.0 4.5 4.6 4.0 – 3.9 3.6 3.5 3.7 6.6 6.1 5.6 8.9 8.9 9.7 6.2 6.1 7.7
Intensity Change (Log2) – – – -0.19 -0.29 -0.99 -0.55 – -0.77 -0.76 -0.8 -0.84 0.29 0.25 0.22 0.29 0.43 0.17 0.1 0.19 0.27


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.9 -2.5 -2.6 -4.0 – -4.4 -5.5 -4.7 -5.5 -1.1 -1.2 -0.9 -2.3 -2.5 -3.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 4.2 3.5 3.3 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 15.3255 20.3535 17.831 16.1218 15.2488 18.3774 25.45 27.5411 26.3309 25.3997 25.4093 25.5225 22.0504 26.4631 24.3087 23.682 22.4614 25.7196
Actin 0.0203 0.0003 0.0223 0.0106 0.003 0.0027 0.0001 0.0001 0.0005 0.0001 0.0001 0.0001 0.023 0.0004 0.0164 0.0231 0.0389 0.0006
Bud 0.0008 0.0001 0.0017 0.0045 0.0008 0.0001 0.0001 0.0016 0.0009 0.0001 0.0001 0 0.0004 0.0001 0.0006 0.0003 0.0004 0.0001
Bud Neck 0.0017 0.0002 0.0022 0.002 0.0008 0.0019 0.0003 0.0003 0.0004 0.0007 0.0006 0.0008 0.0014 0.0001 0.0006 0.0015 0.0012 0.0008
Bud Periphery 0.0005 0 0.0026 0.0051 0.0008 0.0001 0 0.0004 0.0002 0 0 0 0.0004 0 0.0006 0.0002 0.0003 0
Bud Site 0.0031 0.0003 0.0043 0.0008 0.002 0 0 0.0041 0.0002 0.0001 0.0003 0 0.0013 0.0002 0.0024 0.0125 0.0026 0.0001
Cell Periphery 0.0003 0 0.0006 0.0002 0.0002 0 0 0.0001 0.0001 0 0 0 0.0001 0 0.0002 0.0001 0.0002 0
Cytoplasm 0.8227 0.9776 0.8729 0.8469 0.7372 0.9321 0.9483 0.9605 0.956 0.9512 0.957 0.9407 0.8761 0.9882 0.9332 0.9457 0.8187 0.9647
Cytoplasmic Foci 0.0143 0.0028 0.0134 0.016 0.0393 0.001 0.0003 0.0006 0.0006 0.0002 0.0003 0.0004 0.004 0.0011 0.0041 0.0014 0.0352 0.0011
Eisosomes 0.0002 0 0.0002 0.0001 0.0001 0 0 0 0 0 0 0 0.0002 0 0.0002 0.0001 0.0005 0
Endoplasmic Reticulum 0.0089 0.0003 0.0057 0.0023 0.0121 0.0024 0.0017 0.0008 0.0017 0.0006 0.001 0.0016 0.0046 0.0005 0.0046 0.0013 0.0052 0.0005
Endosome 0.0271 0.0003 0.0127 0.0122 0.0627 0.007 0.0002 0.0001 0.0003 0.0001 0.0001 0.0002 0.0054 0.0003 0.0058 0.0023 0.0145 0.0004
Golgi 0.0046 0.0003 0.0034 0.0039 0.0122 0.0029 0 0 0 0 0 0 0.0038 0.0001 0.0028 0.0003 0.0074 0.0001
Lipid Particles 0.005 0.0023 0.0039 0.0166 0.0078 0.0003 0 0 0 0 0 0 0.0034 0 0.0018 0.0001 0.0293 0.0003
Mitochondria 0.0044 0.0004 0.0073 0.0127 0.0419 0.0012 0.0001 0.0001 0.0011 0 0 0.0001 0.003 0.0001 0.0034 0.0003 0.0036 0.0002
None 0.0112 0.0003 0.003 0.0022 0.0069 0.0006 0.0006 0.0005 0.0005 0.0003 0.0003 0.0003 0.0146 0.0004 0.0021 0.0013 0.0054 0.0059
Nuclear Periphery 0.0185 0.0011 0.0069 0.0184 0.0177 0.0075 0.0041 0.0025 0.0041 0.0033 0.0028 0.0038 0.0088 0.001 0.0024 0.0012 0.0051 0.0018
Nucleolus 0.0009 0 0.0008 0.0002 0.0013 0.0001 0 0.0007 0.0001 0 0 0 0.0003 0 0.0001 0 0.0019 0.0003
Nucleus 0.0364 0.0104 0.0201 0.0169 0.0207 0.0381 0.0432 0.0265 0.0325 0.0423 0.0365 0.0508 0.0346 0.0067 0.0113 0.0068 0.0109 0.0216
Peroxisomes 0.0018 0.0026 0.0015 0.0196 0.0068 0.0001 0 0 0 0 0 0 0.0014 0.0001 0.0051 0.0003 0.0112 0.0002
Punctate Nuclear 0.0116 0.0002 0.0088 0.0037 0.0074 0.0011 0.0004 0.0007 0.0005 0.0004 0.0003 0.0005 0.0111 0.0003 0.0007 0.0002 0.006 0.0013
Vacuole 0.0045 0.0002 0.0049 0.0021 0.0149 0.0006 0.0005 0.0004 0.0004 0.0004 0.0005 0.0004 0.0012 0.0001 0.0011 0.0007 0.0011 0.0002
Vacuole Periphery 0.0015 0 0.0008 0.0031 0.0035 0.0004 0 0 0.0001 0 0.0001 0.0001 0.0006 0 0.0004 0.0002 0.0005 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 236.7233 206.3695 228.4048 253.2766 281.5566 168.1565 224.5747 223.7787 227.7029 233.2978
Translational Efficiency 1.9143 2.323 2.0266 1.7357 1.5431 2.7537 1.9436 1.7919 1.7849 1.6809

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1626 1434 666 129 1287 1929 1339 1114 2913 3363 2005 1243

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1711.60 2188.14 2898.68 2921.65 1861.09 2232.29 2933.11 3042.05 1777.65 2213.46 2921.67 3029.55
Standard Deviation 339.23 498.07 506.29 502.93 357.58 425.72 518.90 638.58 355.30 458.49 515.00 626.95
Intensity Change Log 2 — 0.354359 0.760050 0.771438 — 0.262377 0.656283 0.708896 — 0.307176 0.706928 0.739197

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000174 0.000133 0.000173 0.002019 0.000552 0.000153 0.000092 0.000187 0.000341 0.000144 0.000119 0.000377
Bud Neck 0.000351 0.000466 0.001097 0.035835 0.000466 0.000660 0.000766 0.001028 0.000402 0.000577 0.000876 0.004641
Bud Site 0.001200 0.001429 0.001964 0.012823 0.002222 0.002756 0.000728 0.005570 0.001652 0.002190 0.001138 0.006323
Cell Periphery 0.000117 0.000174 0.000386 0.000915 0.000119 0.000091 0.000190 0.000285 0.000118 0.000127 0.000255 0.000350
Cytoplasm 0.831363* 0.770983* 0.558940* 0.389074* 0.783734* 0.780488* 0.653546* 0.743441* 0.810320* 0.776435* 0.622121* 0.706664*
Cytoplasmic Foci 0.005384 0.004887 0.007839 0.022975 0.006743 0.005460 0.003626 0.008925 0.005985 0.005215 0.005025 0.010383
Eisosomes 0.000001 0.000001 0.000001 0.000020 0.000001 0.000001 0.000001 0.000001 0.000001 0.000001 0.000001 0.000003
Endoplasmic Reticulum 0.000181 0.000066 0.000075 0.000741 0.000187 0.000096 0.000027 0.000040 0.000184 0.000083 0.000043 0.000113
Endosome 0.000132 0.000105 0.000114 0.034751 0.000323 0.000270 0.000038 0.000607 0.000216 0.000199 0.000063 0.004150
Golgi 0.000689 0.000485 0.000341 0.024377 0.000607 0.000352 0.000112 0.002200 0.000653 0.000409 0.000188 0.004502
Lipid Particles 0.000332 0.000358 0.000527 0.007247 0.000415 0.000228 0.000281 0.000447 0.000369 0.000284 0.000363 0.001152
Mitochondria 0.000111 0.000302 0.000193 0.001591 0.000282 0.000540 0.000134 0.000109 0.000186 0.000438 0.000154 0.000263
Mitotic Spindle 0.001363 0.000754 0.002228 0.077738 0.001940 0.002073 0.000857 0.006632 0.001618 0.001511 0.001312 0.014012
None 0.010455 0.017647 0.010878 0.004734 0.014986 0.023126 0.015500 0.012958 0.012457 0.020790 0.013965 0.012104
Nuclear Periphery 0.000045 0.000064 0.000222 0.004905 0.000088 0.000053 0.000153 0.000102 0.000064 0.000058 0.000176 0.000600
Nuclear Periphery Foci 0.001752 0.001427 0.000907 0.011087 0.002641 0.001471 0.000574 0.000930 0.002145 0.001452 0.000685 0.001984
Nucleolus 0.000068 0.000082 0.000082 0.001433 0.000068 0.000094 0.000087 0.000099 0.000068 0.000089 0.000085 0.000238
Nucleus 0.141464* 0.197175* 0.406776* 0.269654* 0.180077* 0.178027* 0.316633* 0.203797* 0.158524* 0.186192* 0.346575* 0.210632*
Peroxisomes 0.000020 0.000224 0.000015 0.000191 0.000029 0.000082 0.000009 0.000146 0.000024 0.000143 0.000011 0.000151
Vacuole 0.004062 0.003154 0.007177 0.094770 0.004131 0.003279 0.006616 0.011295 0.004092 0.003226 0.006802 0.019959
Vacuole Periphery 0.000738 0.000084 0.000065 0.003117 0.000386 0.000699 0.000031 0.001199 0.000583 0.000436 0.000042 0.001398

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.27 -3.33 -3.17 -3.67 -1.70 0.92 0.88 0.90 -0.19 0.31 1.04 0.58 0.72 -1.95 0.95
Bud Neck -3.19 -8.46 -6.36 -6.21 -4.94 -1.31 -11.60 -9.64 -5.02 4.20 -2.55 -14.50 -9.72 -8.14 -0.44
Bud Site -1.05 -2.43 -8.85 -7.93 -4.85 -0.62 -0.09 -2.82 -2.48 -3.65 -1.31 -2.18 -5.18 -4.24 -3.95
Cell Periphery -3.05 -10.03 -6.87 -6.38 -2.36 1.38 -9.82 -8.40 -9.41 1.51 -1.39 -13.74 -10.41 -10.03 2.22
Cytoplasm 12.25 34.97 19.34 15.96 4.08 0.87 26.25 9.97 10.07 -14.71 9.74 44.03 19.50 13.81 -14.70
Cytoplasmic Foci -0.71 -7.47 -6.75 -6.61 -3.06 0.96 -4.42 -5.99 -6.97 -2.18 0.13 -8.67 -8.78 -8.94 -1.91
Eisosomes -0.42 -7.06 -7.57 -7.15 -5.57 0.54 -5.17 -4.30 -7.14 1.20 -0.09 -9.15 -8.88 -7.72 -1.26
Endoplasmic Reticulum 3.93 1.94 0.01 -3.11 -1.64 2.39 4.38 5.18 3.20 3.90 4.06 5.10 6.64 2.96 2.90
Endosome 0.81 -1.57 -1.36 -1.38 -1.32 0.49 2.12 0.77 0.26 -1.03 0.38 1.55 -1.14 -1.26 -1.53
Golgi 0.92 0.72 -0.92 -1.06 -1.02 1.71 2.94 -0.17 -0.69 -0.95 1.88 2.90 -0.35 -0.86 -1.04
Lipid Particles -0.62 -5.87 -6.70 -6.61 -4.77 1.46 -5.05 -5.56 -7.28 -1.48 0.59 -7.87 -8.37 -8.99 -2.60
Mitochondria -0.92 -6.86 -1.86 0.10 0.12 -0.63 0.53 1.17 1.33 7.92 -1.09 -0.53 1.00 1.53 7.54
Mitotic Spindle 0.76 -1.37 -1.82 -1.94 -1.42 -0.33 -0.47 -1.95 -1.80 -1.69 -0.08 -1.38 -2.79 -2.79 -2.00
None -5.45 0.66 7.41 12.12 8.54 -4.26 1.91 4.07 10.08 3.57 -7.24 1.17 4.36 12.49 4.19
Nuclear Periphery -5.05 -12.82 -4.84 -4.72 -3.04 3.76 -15.05 -9.47 -13.13 7.84 -0.37 -20.75 -8.98 -8.94 2.88
Nuclear Periphery Foci 1.21 1.10 -4.23 -4.71 -4.66 2.67 5.16 4.13 0.07 -1.84 2.63 5.10 2.23 -1.05 -3.11
Nucleolus -1.83 -6.48 -2.81 -2.62 -2.23 -2.66 -10.25 -4.52 -0.36 5.58 -3.23 -11.96 -4.84 -2.57 1.20
Nucleus -11.88 -31.51 -5.95 -1.91 10.45 0.03 -23.09 -2.54 -2.84 19.71 -8.71 -39.33 -8.30 -2.23 25.12
Peroxisomes -1.01 -1.28 -4.88 0.66 -4.15 -1.85 0.30 -1.09 0.61 -1.21 -1.41 -0.29 -1.54 0.88 -1.42
Vacuole -0.50 -8.20 -11.57 -11.55 -10.34 0.09 -13.08 -15.36 -15.40 -3.99 -0.22 -15.47 -17.32 -17.26 -8.92
Vacuole Periphery 1.49 1.43 0.82 -1.82 -1.66 -0.52 1.76 0.07 0.51 -0.96 0.50 1.96 0.58 0.11 -1.01
Standard name
Human Ortholog
Description Guanylate kinase; converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
Localization
Cell Percentages cytoplasm (94%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Guk1

Guk1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Guk1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available