Standard name | |
---|---|
Human Ortholog | |
Description | Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 22 | 66 | 21 | 5 | 4 | 3 | 2 | 10 | 9 | 3 | 6 | 8 | 65 | 73 | 35 | 31 | 30 | 20 | 1 | 11 | 9 | 6 | 14 | 11 |
Bud | 3 | 8 | 7 | 4 | 2 | 20 | 15 | 24 | 13 | 14 | 18 | 21 | 7 | 7 | 5 | 1 | 5 | 3 | 1 | 1 | 0 | 0 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 0 | 1 | 0 | 3 | 1 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 20 | 36 | 17 | 23 | 20 | 27 | 18 | 19 | 14 | 21 | 18 | 13 | 17 | 17 | 25 | 43 | 25 | 28 | 0 | 2 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 21 | 88 | 32 | 3 | 9 | 15 | 15 | 22 | 14 | 7 | 8 | 10 | 44 | 40 | 40 | 22 | 10 | 8 | 1 | 10 | 2 | 4 | 8 | 8 |
Endosome | 89 | 85 | 175 | 107 | 140 | 52 | 53 | 61 | 16 | 16 | 15 | 41 | 175 | 174 | 136 | 134 | 61 | 62 | 194 | 258 | 203 | 106 | 167 | 116 |
Golgi | 64 | 136 | 117 | 61 | 38 | 25 | 29 | 22 | 14 | 11 | 12 | 11 | 376 | 399 | 494 | 151 | 145 | 99 | 82 | 170 | 174 | 101 | 255 | 232 |
Mitochondria | 28 | 76 | 47 | 84 | 70 | 272 | 341 | 325 | 263 | 337 | 210 | 303 | 24 | 24 | 26 | 45 | 30 | 26 | 6 | 11 | 6 | 14 | 16 | 13 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 1 | 2 | 5 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 4 | 2 | 2 | 4 | 0 | 5 | 3 | 5 | 4 | 3 | 1 | 2 | 3 | 4 | 7 | 7 | 6 | 4 | 1 | 0 | 1 | 0 | 0 | 1 |
Nucleolus | 5 | 2 | 3 | 6 | 7 | 14 | 14 | 17 | 9 | 18 | 6 | 11 | 5 | 5 | 5 | 10 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 4 | 4 | 3 | 1 | 1 | 3 | 2 | 1 | 2 | 12 | 3 | 5 | 0 | 1 | 0 | 0 | 1 | 2 | 9 | 4 | 5 | 3 |
SpindlePole | 3 | 0 | 27 | 6 | 4 | 7 | 7 | 14 | 1 | 5 | 6 | 9 | 26 | 26 | 7 | 10 | 7 | 7 | 3 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 70 | 56 | 20 | 18 | 22 | 31 | 27 | 14 | 19 | 27 | 10 | 19 | 130 | 134 | 143 | 51 | 49 | 45 | 14 | 19 | 0 | 3 | 8 | 6 |
Unique Cell Count | 249 | 405 | 343 | 248 | 253 | 381 | 436 | 430 | 310 | 397 | 256 | 373 | 711 | 725 | 768 | 393 | 284 | 220 | 310 | 494 | 411 | 244 | 486 | 399 |
Labelled Cell Count | 329 | 558 | 472 | 327 | 320 | 477 | 528 | 537 | 382 | 470 | 315 | 463 | 875 | 908 | 924 | 508 | 372 | 309 | 310 | 494 | 411 | 244 | 486 | 399 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.7 | 9.9 | 9.2 | 8.7 | 8.1 | 7.0 | 6.8 | 6.7 | 6.7 | 6.4 | 6.8 | 6.7 | 11.3 | 11.3 | 11.6 | 9.4 | 11.1 | 11.3 | 9.0 | 10.3 | 10.8 |
Std Deviation (1e-4) | 1.5 | 1.5 | 1.4 | 1.8 | 1.7 | 1.6 | 1.4 | 1.2 | 1.3 | 1.3 | 1.6 | 1.3 | 1.6 | 1.6 | 1.6 | 1.8 | 2.2 | 2.7 | 1.3 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.19 | -0.4 | -0.44 | -0.44 | -0.46 | -0.52 | -0.43 | -0.45 | 0.3 | 0.3 | 0.34 | 0.03 | 0.28 | 0.31 | -0.02 | 0.17 | 0.23 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.4 | -2.7 | -4.0 | -4.6 | -2.7 | -2.0 | -4.1 | -2.2 | -2.7 | 1.7 | 2.1 | -1.1 | 0.9 | 2.0 | 1.3 |
Bud | 0 | 0 | 2.3 | 1.2 | 2.5 | 1.6 | 1.2 | 3.0 | 2.5 | 0 | 0 | 0 | 0 | -0.3 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.1 | 1.5 | 1.2 | -0.6 | -0.4 | -0.3 | 0.2 | 1.1 | -1.0 | -2.2 | -2.3 | -1.4 | 3.0 | 1.9 | 3.3 |
Endoplasmic Reticulum | -4.1 | -2.8 | -2.9 | -3.4 | -2.3 | -2.4 | -4.6 | -3.0 | -3.8 | -1.8 | -2.3 | -2.6 | -1.9 | -2.9 | -2.6 |
Endosome | -1.9 | 1.0 | -10.8 | -11.8 | -11.0 | -12.9 | -14.6 | -11.7 | -11.7 | -8.5 | -8.8 | -11.4 | -4.6 | -7.6 | -5.4 |
Golgi | -2.5 | -5.3 | -9.3 | -9.7 | -10.4 | -9.4 | -11.2 | -8.7 | -10.9 | 5.7 | 6.4 | 9.4 | 1.2 | 4.3 | 2.6 |
Mitochondria | 5.8 | 4.2 | 15.6 | 17.9 | 17.1 | 18.2 | 19.3 | 16.7 | 18.1 | -6.3 | -6.4 | -6.4 | -0.9 | -1.2 | -0.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 2.5 | 2.2 | 2.7 | 1.9 | 3.0 | 0 | 2.0 | 0 | 0 | 0 | 1.7 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -2.8 | -3.4 | -3.8 | -4.2 | -2.8 | -4.8 | -4.4 | -2.9 | -3.3 | -2.9 | -3.0 | -6.2 | -3.3 | -3.0 | -2.3 |
Vacuole | 0.7 | 1.4 | 1.2 | 0.2 | -1.7 | 0.2 | 0.5 | -1.1 | -0.4 | 5.4 | 5.5 | 5.6 | 3.3 | 4.5 | 5.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0859 | 6.4829 | 6.2517 | 5.678 | 5.5421 | 6.0762 | 8.0263 | 8.7839 | 8.3565 | 7.0546 | 7.6209 | 8.2274 | 8.8583 | 10.0322 | 9.2855 | 9.3886 | 9.0531 | 9.6688 |
Actin | 0.0154 | 0.0008 | 0.0127 | 0.0003 | 0.0023 | 0.0014 | 0.0236 | 0.0008 | 0.0032 | 0.0001 | 0.0003 | 0.0005 | 0.0049 | 0.0017 | 0.0025 | 0.0002 | 0.0004 | 0.0019 |
Bud | 0.0006 | 0.0005 | 0.0003 | 0.012 | 0.0009 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0011 | 0.0003 | 0.0008 | 0.0003 | 0.0004 | 0.0003 | 0.0006 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0002 |
Bud Periphery | 0.0007 | 0.0004 | 0.0002 | 0.0053 | 0.0014 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0.0017 | 0.0006 | 0.0007 | 0.0016 | 0.0047 | 0.0001 | 0.0005 | 0.0002 | 0.0007 | 0 | 0 | 0.0001 | 0.0002 | 0.0003 | 0.0002 | 0 | 0 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.012 | 0.0201 | 0.0073 | 0.0036 | 0.0041 | 0.0081 | 0.0043 | 0.0126 | 0.0111 | 0.0013 | 0.0023 | 0.0021 | 0.0034 | 0.0064 | 0.0026 | 0.0016 | 0.0016 | 0.0021 |
Cytoplasmic Foci | 0.1097 | 0.1276 | 0.1198 | 0.0929 | 0.0664 | 0.1012 | 0.1256 | 0.1638 | 0.1394 | 0.0797 | 0.1319 | 0.1172 | 0.1123 | 0.1343 | 0.1263 | 0.104 | 0.1178 | 0.1142 |
Eisosomes | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0094 | 0.0088 | 0.0018 | 0.0011 | 0.0019 | 0.0058 | 0.0045 | 0.0026 | 0.0022 | 0.001 | 0.0011 | 0.0031 | 0.002 | 0.0039 | 0.0035 | 0.0007 | 0.0009 | 0.0021 |
Endosome | 0.519 | 0.5888 | 0.5159 | 0.6224 | 0.5443 | 0.4488 | 0.4799 | 0.4582 | 0.4049 | 0.5053 | 0.4265 | 0.417 | 0.4607 | 0.4903 | 0.4428 | 0.4927 | 0.4595 | 0.3998 |
Golgi | 0.2106 | 0.2051 | 0.271 | 0.2094 | 0.2276 | 0.3486 | 0.2693 | 0.2929 | 0.3814 | 0.3869 | 0.382 | 0.3897 | 0.3055 | 0.2901 | 0.372 | 0.3736 | 0.3515 | 0.4119 |
Lipid Particles | 0.0489 | 0.0206 | 0.0286 | 0.0124 | 0.0389 | 0.0574 | 0.0657 | 0.0514 | 0.0278 | 0.0126 | 0.0401 | 0.0434 | 0.0806 | 0.0572 | 0.0286 | 0.0174 | 0.0598 | 0.0518 |
Mitochondria | 0.0142 | 0.0076 | 0.0059 | 0.0053 | 0.0375 | 0.0083 | 0.0061 | 0.0027 | 0.0108 | 0.0036 | 0.004 | 0.0083 | 0.004 | 0.0023 | 0.0023 | 0.002 | 0.0007 | 0.0045 |
None | 0.0029 | 0.0008 | 0.0002 | 0.0006 | 0.0003 | 0.0016 | 0.0003 | 0.0001 | 0.0024 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0018 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0.0158 | 0.0014 | 0.0021 | 0.0007 | 0.0008 | 0.0032 | 0.004 | 0.0003 | 0.0012 | 0.0002 | 0.0008 | 0.0005 | 0.0006 | 0.0005 | 0.0024 | 0.0002 | 0.0002 | 0.0003 |
Nucleolus | 0.0005 | 0.0001 | 0.0003 | 0.0015 | 0.0006 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0037 | 0.0003 | 0.0005 | 0.0087 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Peroxisomes | 0.0121 | 0.0033 | 0.0073 | 0.0024 | 0.0027 | 0.0049 | 0.0074 | 0.0064 | 0.0068 | 0.0026 | 0.0053 | 0.0122 | 0.0136 | 0.0045 | 0.0108 | 0.0023 | 0.0038 | 0.0057 |
Punctate Nuclear | 0.0027 | 0.0003 | 0.0004 | 0.0006 | 0.0002 | 0.0004 | 0.0007 | 0.0002 | 0.0015 | 0.0001 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Vacuole | 0.0116 | 0.0087 | 0.0185 | 0.0112 | 0.0448 | 0.0045 | 0.0041 | 0.0054 | 0.0027 | 0.0031 | 0.0029 | 0.0024 | 0.0076 | 0.0032 | 0.0025 | 0.0024 | 0.0014 | 0.0022 |
Vacuole Periphery | 0.007 | 0.0039 | 0.0056 | 0.0076 | 0.0189 | 0.0049 | 0.0025 | 0.0019 | 0.0021 | 0.0033 | 0.0022 | 0.003 | 0.0037 | 0.0026 | 0.0027 | 0.0027 | 0.002 | 0.0028 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 39.1587 | 27.4858 | 36.943 | 49.6959 | 29.3343 | 30.7298 | 47.8474 | 55.0322 | 50.1556 | 55.8558 |
Translational Efficiency | 0.5594 | 0.817 | 0.7635 | 0.4815 | 0.6434 | 1.084 | 0.6359 | 0.498 | 0.6506 | 0.5359 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
120 | 475 | 2064 | 1699 | 1978 | 2069 | 898 | 1060 | 2098 | 2544 | 2962 | 2759 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1069.21 | 1028.32 | 1118.66 | 1191.50 | 996.73 | 1026.74 | 1102.84 | 1083.65 | 1000.88 | 1027.04 | 1113.86 | 1150.06 |
Standard Deviation | 170.93 | 174.79 | 176.74 | 162.12 | 159.08 | 146.84 | 164.20 | 149.58 | 160.67 | 152.45 | 173.19 | 165.93 |
Intensity Change Log 2 | — | -0.056256 | 0.065226 | 0.156234 | — | 0.042796 | 0.145949 | 0.120624 | — | -0.007618 | 0.104736 | 0.139163 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.003141 | 0.002102 | 0.003547 | 0.007862 | 0.003686 | 0.001673 | 0.015212 | 0.000760 | 0.003655 | 0.001753 | 0.007084 | 0.005134 |
Bud Neck | 0.001946 | 0.000384 | 0.000473 | 0.001021 | 0.000154 | 0.000345 | 0.001541 | 0.000326 | 0.000256 | 0.000352 | 0.000796 | 0.000754 |
Bud Site | 0.025686 | 0.001999 | 0.002617 | 0.013352 | 0.000992 | 0.000812 | 0.016907 | 0.004643 | 0.002404 | 0.001034 | 0.006949 | 0.010006 |
Cell Periphery | 0.000641 | 0.000118 | 0.000123 | 0.000282 | 0.000076 | 0.000075 | 0.000300 | 0.000066 | 0.000109 | 0.000083 | 0.000177 | 0.000199 |
Cytoplasm | 0.004839 | 0.000639 | 0.000628 | 0.004151 | 0.000335 | 0.000340 | 0.004618 | 0.004143 | 0.000593 | 0.000396 | 0.001838 | 0.004148 |
Cytoplasmic Foci | 0.145895 | 0.117574 | 0.096620 | 0.159832 | 0.118112 | 0.089391 | 0.152661 | 0.358184* | 0.119701 | 0.094653 | 0.113610 | 0.236038* |
Eisosomes | 0.000054 | 0.000027 | 0.000033 | 0.000052 | 0.000056 | 0.000029 | 0.000102 | 0.000015 | 0.000056 | 0.000029 | 0.000054 | 0.000038 |
Endoplasmic Reticulum | 0.002509 | 0.001123 | 0.002429 | 0.003308 | 0.000594 | 0.000450 | 0.010067 | 0.001342 | 0.000704 | 0.000576 | 0.004745 | 0.002553 |
Endosome | 0.420911* | 0.528675* | 0.418225* | 0.359100* | 0.374590* | 0.564645* | 0.380849* | 0.175337 | 0.377240* | 0.557929* | 0.406894* | 0.288498* |
Golgi | 0.204521* | 0.290993* | 0.343534* | 0.333370* | 0.453037* | 0.284338* | 0.311814* | 0.409652* | 0.438822* | 0.285580* | 0.333917* | 0.362677* |
Lipid Particles | 0.010084 | 0.008904 | 0.042519 | 0.013344 | 0.024023 | 0.015372 | 0.035315 | 0.010606 | 0.023225 | 0.014164 | 0.040335 | 0.012292 |
Mitochondria | 0.002066 | 0.004658 | 0.001162 | 0.003591 | 0.008053 | 0.007579 | 0.001483 | 0.002724 | 0.007710 | 0.007034 | 0.001260 | 0.003258 |
Mitotic Spindle | 0.005624 | 0.002007 | 0.001413 | 0.011070 | 0.000217 | 0.000721 | 0.005625 | 0.006408 | 0.000526 | 0.000961 | 0.002690 | 0.009279 |
None | 0.000013 | 0.000011 | 0.000012 | 0.000016 | 0.000015 | 0.000013 | 0.000028 | 0.000137 | 0.000014 | 0.000013 | 0.000017 | 0.000063 |
Nuclear Periphery | 0.000255 | 0.000103 | 0.000400 | 0.000201 | 0.000037 | 0.000095 | 0.000229 | 0.000095 | 0.000050 | 0.000097 | 0.000348 | 0.000161 |
Nuclear Periphery Foci | 0.001415 | 0.000439 | 0.005134 | 0.002143 | 0.000289 | 0.001393 | 0.005347 | 0.002213 | 0.000354 | 0.001215 | 0.005199 | 0.002170 |
Nucleolus | 0.000082 | 0.000037 | 0.000011 | 0.000017 | 0.000005 | 0.000022 | 0.000017 | 0.000389 | 0.000010 | 0.000024 | 0.000013 | 0.000159 |
Nucleus | 0.000212 | 0.000028 | 0.000023 | 0.000038 | 0.000008 | 0.000023 | 0.000084 | 0.000276 | 0.000020 | 0.000024 | 0.000041 | 0.000129 |
Peroxisomes | 0.017286 | 0.001888 | 0.000528 | 0.002616 | 0.001291 | 0.001812 | 0.002097 | 0.001994 | 0.002205 | 0.001826 | 0.001004 | 0.002377 |
Vacuole | 0.116002 | 0.025552 | 0.064374 | 0.069614 | 0.007264 | 0.018438 | 0.046833 | 0.014045 | 0.013484 | 0.019766 | 0.059056 | 0.048264 |
Vacuole Periphery | 0.036817 | 0.012738 | 0.016193 | 0.015020 | 0.007167 | 0.012434 | 0.008870 | 0.006646 | 0.008863 | 0.012491 | 0.013973 | 0.011803 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 0.75 | -0.28 | -2.84 | -4.09 | -3.12 | 2.04 | -4.21 | 3.71 | 1.42 | 5.49 | 2.12 | -2.85 | -1.49 | -3.79 | 1.55 |
Bud Neck | 3.56 | 3.35 | 1.99 | -5.40 | -4.68 | -1.26 | -3.90 | -2.00 | 0.12 | 3.34 | -0.77 | -4.41 | -6.34 | -2.91 | 0.22 |
Bud Site | 2.82 | 2.75 | 1.41 | -9.31 | -9.32 | 0.72 | -7.90 | -3.83 | -4.00 | 5.58 | 2.51 | -5.55 | -8.17 | -11.14 | -3.09 |
Cell Periphery | 1.89 | 1.88 | 1.24 | -1.85 | -1.86 | 0.11 | -6.71 | 1.05 | 0.62 | 6.88 | 1.29 | -3.43 | -1.65 | -2.17 | -0.40 |
Cytoplasm | 3.49 | 3.54 | 0.36 | -6.45 | -6.89 | -0.06 | -5.59 | -3.90 | -3.89 | 0.33 | 1.62 | -4.88 | -7.25 | -7.64 | -4.34 |
Cytoplasmic Foci | 2.30 | 4.32 | -1.17 | -6.90 | -16.21 | 8.68 | -6.90 | -38.54 | -43.98 | -28.82* | 7.90 | 1.95 | -27.35 | -35.06 | -30.27 |
Eisosomes | 2.19 | 1.74 | 0.13 | -4.81 | -4.07 | 4.87 | -3.37 | 9.16 | 3.75 | 6.75 | 5.41 | 0.37 | 3.60 | -2.41 | 3.13 |
Endoplasmic Reticulum | 1.85 | 0.10 | -0.87 | -3.02 | -1.25 | 1.01 | -5.63 | -2.89 | -3.83 | 5.15 | 0.85 | -7.12 | -4.39 | -4.82 | 3.20 |
Endosome | -4.12 | 0.10 | 2.57 | 13.52 | 7.94 | -24.88 | -0.58 | 28.28 | 54.55* | 23.29* | -25.17 | -4.32 | 13.41 | 41.95 | 19.83 |
Golgi | -3.81 | -6.55 | -5.96 | -3.52 | 1.43 | 23.76* | 15.12 | 5.03 | -14.91 | -9.47 | 22.57* | 15.32 | 11.06 | -12.09 | -4.41 |
Lipid Particles | 0.28 | -7.09 | -0.81 | -1.75 | 10.46 | 3.37 | -3.34 | 5.72 | 2.48 | 8.35 | 3.85 | -6.30 | 5.06 | 1.12 | 13.26 |
Mitochondria | -1.38 | 1.42 | -1.58 | 0.53 | -2.75 | 0.36 | 5.55 | 4.01 | 4.33 | -1.30 | 0.55 | 6.18 | 3.81 | 4.02 | -3.06 |
Mitotic Spindle | 1.16 | 1.57 | -1.79 | -3.98 | -6.07 | -1.27 | -3.54 | -5.23 | -4.59 | -0.30 | -0.88 | -3.78 | -8.21 | -7.32 | -5.52 |
None | 0.54 | 0.42 | -0.88 | -1.40 | -1.75 | 0.36 | -1.19 | -1.38 | -1.39 | -1.20 | 0.52 | -0.70 | -1.41 | -1.46 | -1.32 |
Nuclear Periphery | 1.79 | -1.69 | 0.74 | -1.55 | 2.83 | -3.24 | -2.50 | -2.04 | -0.02 | 1.66 | -2.49 | -5.98 | -5.46 | -2.48 | 3.46 |
Nuclear Periphery Foci | 2.95 | -5.24 | -1.87 | -6.37 | 4.31 | -2.82 | -3.95 | -4.83 | -1.50 | 2.39 | -2.69 | -8.17 | -8.13 | -2.57 | 4.81 |
Nucleolus | 1.41 | 3.01 | 2.76 | 0.96 | -1.87 | -1.94 | -1.57 | -1.58 | -1.51 | -1.53 | -1.84 | -0.94 | -1.61 | -1.45 | -1.57 |
Nucleus | 2.34 | 2.40 | 2.20 | -1.07 | -1.88 | -1.61 | -2.11 | -1.65 | -1.55 | -1.12 | -0.49 | -1.78 | -1.76 | -1.69 | -1.36 |
Peroxisomes | 2.51 | 2.75 | 2.40 | -0.98 | -5.91 | -1.32 | -1.72 | -1.20 | -0.32 | 0.09 | 0.76 | 2.81 | -0.37 | -1.34 | -4.17 |
Vacuole | 6.08 | 3.47 | 3.04 | -10.68 | -1.54 | -8.35 | -12.31 | -4.45 | 2.39 | 9.59 | -3.89 | -19.33 | -15.22 | -12.83 | 3.65 |
Vacuole Periphery | 3.58 | 3.17 | 3.34 | -1.08 | 0.97 | -5.37 | -1.46 | 0.57 | 5.16 | 1.73 | -3.67 | -5.70 | -3.29 | 0.68 | 2.40 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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