Standard name
Human Ortholog
Description Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.09 0.16 0.06 0 0 0 0 0 0 0 0 0 0.09 0.1 0.05 0.08 0.11 0.09 0 0 0 0 0 0
Bud 0 0 0 0 0 0.05 0 0.06 0 0 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.08 0.09 0.05 0.09 0.08 0.07 0 0 0.05 0.05 0.07 0 0 0 0 0.11 0.09 0.13 0 0 0 0 0 0
Endoplasmic Reticulum 0.08 0.22 0.09 0 0 0 0 0.05 0.05 0 0 0 0.06 0.06 0.05 0.06 0 0 0 0 0 0 0 0
Endosome 0.36 0.21 0.51 0.43 0.55 0.14 0.12 0.14 0.05 0 0.06 0.11 0.25 0.24 0.18 0.34 0.21 0.28 0.63 0.52 0.49 0.44 0.34 0.29
Golgi 0.26 0.34 0.34 0.25 0.15 0.07 0.07 0.05 0.05 0 0.05 0 0.53 0.55 0.64 0.38 0.51 0.45 0.27 0.34 0.43 0.42 0.53 0.58
Mitochondria 0.11 0.19 0.14 0.34 0.28 0.71 0.78 0.76 0.85 0.85 0.82 0.81 0 0 0 0.11 0.11 0.12 0 0 0 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.28 0.14 0.06 0.07 0.09 0.08 0.06 0 0.06 0.07 0 0.05 0.18 0.18 0.19 0.13 0.17 0.2 0.05 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 22 66 21 5 4 3 2 10 9 3 6 8 65 73 35 31 30 20 1 11 9 6 14 11
Bud 3 8 7 4 2 20 15 24 13 14 18 21 7 7 5 1 5 3 1 1 0 0 1 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Bud Site 0 0 0 0 0 1 0 0 1 1 1 1 0 0 0 0 0 0
Cell Periphery 0 2 0 1 0 3 1 0 1 1 2 1 0 0 1 1 1 1 0 0 0 0 0 0
Cytoplasm 20 36 17 23 20 27 18 19 14 21 18 13 17 17 25 43 25 28 0 2 0 0 0 0
Endoplasmic Reticulum 21 88 32 3 9 15 15 22 14 7 8 10 44 40 40 22 10 8 1 10 2 4 8 8
Endosome 89 85 175 107 140 52 53 61 16 16 15 41 175 174 136 134 61 62 194 258 203 106 167 116
Golgi 64 136 117 61 38 25 29 22 14 11 12 11 376 399 494 151 145 99 82 170 174 101 255 232
Mitochondria 28 76 47 84 70 272 341 325 263 337 210 303 24 24 26 45 30 26 6 11 6 14 16 13
Nucleus 0 0 0 1 1 1 2 1 2 5 0 1 0 0 0 1 0 1 0 0 0 0 0 0
Nuclear Periphery 4 2 2 4 0 5 3 5 4 3 1 2 3 4 7 7 6 4 1 0 1 0 0 1
Nucleolus 5 2 3 6 7 14 14 17 9 18 6 11 5 5 5 10 3 5 0 0 0 0 0 0
Peroxisomes 0 1 4 4 3 1 1 3 2 1 2 12 3 5 0 1 0 0 1 2 9 4 5 3
SpindlePole 3 0 27 6 4 7 7 14 1 5 6 9 26 26 7 10 7 7 3 1 0 0 0 0
Vac/Vac Membrane 70 56 20 18 22 31 27 14 19 27 10 19 130 134 143 51 49 45 14 19 0 3 8 6
Unique Cell Count 249 405 343 248 253 381 436 430 310 397 256 373 711 725 768 393 284 220 310 494 411 244 486 399
Labelled Cell Count 329 558 472 327 320 477 528 537 382 470 315 463 875 908 924 508 372 309 310 494 411 244 486 399


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 9.9 9.2 8.7 8.1 7.0 6.8 6.7 6.7 6.4 6.8 6.7 11.3 11.3 11.6 9.4 11.1 11.3 9.0 10.3 10.8
Std Deviation (1e-4) 1.5 1.5 1.4 1.8 1.7 1.6 1.4 1.2 1.3 1.3 1.6 1.3 1.6 1.6 1.6 1.8 2.2 2.7 1.3 1.5 1.7
Intensity Change (Log2) -0.07 -0.19 -0.4 -0.44 -0.44 -0.46 -0.52 -0.43 -0.45 0.3 0.3 0.34 0.03 0.28 0.31 -0.02 0.17 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.4 -2.7 -4.0 -4.6 -2.7 -2.0 -4.1 -2.2 -2.7 1.7 2.1 -1.1 0.9 2.0 1.3
Bud 0 0 2.3 1.2 2.5 1.6 1.2 3.0 2.5 0 0 0 0 -0.3 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 1.5 1.2 -0.6 -0.4 -0.3 0.2 1.1 -1.0 -2.2 -2.3 -1.4 3.0 1.9 3.3
Endoplasmic Reticulum -4.1 -2.8 -2.9 -3.4 -2.3 -2.4 -4.6 -3.0 -3.8 -1.8 -2.3 -2.6 -1.9 -2.9 -2.6
Endosome -1.9 1.0 -10.8 -11.8 -11.0 -12.9 -14.6 -11.7 -11.7 -8.5 -8.8 -11.4 -4.6 -7.6 -5.4
Golgi -2.5 -5.3 -9.3 -9.7 -10.4 -9.4 -11.2 -8.7 -10.9 5.7 6.4 9.4 1.2 4.3 2.6
Mitochondria 5.8 4.2 15.6 17.9 17.1 18.2 19.3 16.7 18.1 -6.3 -6.4 -6.4 -0.9 -1.2 -0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 2.5 2.2 2.7 1.9 3.0 0 2.0 0 0 0 1.7 0 0
Peroxisomes 0 0 0 0 0 0 0 0 1.9 0 0 0 0 0 0
SpindlePole -2.8 -3.4 -3.8 -4.2 -2.8 -4.8 -4.4 -2.9 -3.3 -2.9 -3.0 -6.2 -3.3 -3.0 -2.3
Vacuole 0.7 1.4 1.2 0.2 -1.7 0.2 0.5 -1.1 -0.4 5.4 5.5 5.6 3.3 4.5 5.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.0859 6.4829 6.2517 5.678 5.5421 6.0762 8.0263 8.7839 8.3565 7.0546 7.6209 8.2274 8.8583 10.0322 9.2855 9.3886 9.0531 9.6688
Actin 0.0154 0.0008 0.0127 0.0003 0.0023 0.0014 0.0236 0.0008 0.0032 0.0001 0.0003 0.0005 0.0049 0.0017 0.0025 0.0002 0.0004 0.0019
Bud 0.0006 0.0005 0.0003 0.012 0.0009 0.0001 0.0002 0.0001 0.0002 0 0 0 0.0001 0.0001 0.0001 0 0 0
Bud Neck 0.0011 0.0003 0.0008 0.0003 0.0004 0.0003 0.0006 0.0002 0.0006 0.0001 0.0001 0.0002 0.0002 0.0002 0.0004 0.0001 0.0001 0.0002
Bud Periphery 0.0007 0.0004 0.0002 0.0053 0.0014 0.0001 0.0002 0 0.0001 0 0 0 0.0001 0 0 0 0 0
Bud Site 0.0017 0.0006 0.0007 0.0016 0.0047 0.0001 0.0005 0.0002 0.0007 0 0 0.0001 0.0002 0.0003 0.0002 0 0 0.0001
Cell Periphery 0.0002 0.0001 0.0002 0.0002 0.0006 0.0001 0.0001 0 0.0001 0 0 0 0.0001 0.0001 0 0 0 0
Cytoplasm 0.012 0.0201 0.0073 0.0036 0.0041 0.0081 0.0043 0.0126 0.0111 0.0013 0.0023 0.0021 0.0034 0.0064 0.0026 0.0016 0.0016 0.0021
Cytoplasmic Foci 0.1097 0.1276 0.1198 0.0929 0.0664 0.1012 0.1256 0.1638 0.1394 0.0797 0.1319 0.1172 0.1123 0.1343 0.1263 0.104 0.1178 0.1142
Eisosomes 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0094 0.0088 0.0018 0.0011 0.0019 0.0058 0.0045 0.0026 0.0022 0.001 0.0011 0.0031 0.002 0.0039 0.0035 0.0007 0.0009 0.0021
Endosome 0.519 0.5888 0.5159 0.6224 0.5443 0.4488 0.4799 0.4582 0.4049 0.5053 0.4265 0.417 0.4607 0.4903 0.4428 0.4927 0.4595 0.3998
Golgi 0.2106 0.2051 0.271 0.2094 0.2276 0.3486 0.2693 0.2929 0.3814 0.3869 0.382 0.3897 0.3055 0.2901 0.372 0.3736 0.3515 0.4119
Lipid Particles 0.0489 0.0206 0.0286 0.0124 0.0389 0.0574 0.0657 0.0514 0.0278 0.0126 0.0401 0.0434 0.0806 0.0572 0.0286 0.0174 0.0598 0.0518
Mitochondria 0.0142 0.0076 0.0059 0.0053 0.0375 0.0083 0.0061 0.0027 0.0108 0.0036 0.004 0.0083 0.004 0.0023 0.0023 0.002 0.0007 0.0045
None 0.0029 0.0008 0.0002 0.0006 0.0003 0.0016 0.0003 0.0001 0.0024 0 0.0001 0.0001 0.0002 0.0018 0 0 0 0
Nuclear Periphery 0.0158 0.0014 0.0021 0.0007 0.0008 0.0032 0.004 0.0003 0.0012 0.0002 0.0008 0.0005 0.0006 0.0005 0.0024 0.0002 0.0002 0.0003
Nucleolus 0.0005 0.0001 0.0003 0.0015 0.0006 0 0 0.0001 0.0001 0 0 0 0 0 0 0 0 0
Nucleus 0.0037 0.0003 0.0005 0.0087 0.0009 0.0001 0.0003 0.0001 0.0006 0 0.0001 0 0.0001 0.0001 0.0001 0 0 0
Peroxisomes 0.0121 0.0033 0.0073 0.0024 0.0027 0.0049 0.0074 0.0064 0.0068 0.0026 0.0053 0.0122 0.0136 0.0045 0.0108 0.0023 0.0038 0.0057
Punctate Nuclear 0.0027 0.0003 0.0004 0.0006 0.0002 0.0004 0.0007 0.0002 0.0015 0.0001 0.0002 0.0001 0.0003 0.0003 0.0002 0.0001 0.0001 0.0001
Vacuole 0.0116 0.0087 0.0185 0.0112 0.0448 0.0045 0.0041 0.0054 0.0027 0.0031 0.0029 0.0024 0.0076 0.0032 0.0025 0.0024 0.0014 0.0022
Vacuole Periphery 0.007 0.0039 0.0056 0.0076 0.0189 0.0049 0.0025 0.0019 0.0021 0.0033 0.0022 0.003 0.0037 0.0026 0.0027 0.0027 0.002 0.0028

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 39.1587 27.4858 36.943 49.6959 29.3343 30.7298 47.8474 55.0322 50.1556 55.8558
Translational Efficiency 0.5594 0.817 0.7635 0.4815 0.6434 1.084 0.6359 0.498 0.6506 0.5359

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism
Localization
Cell Percentages Golgi (11%), endosome (9%), mixed (54%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Nhx1

Nhx1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Nhx1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available