Standard name | |
---|---|
Human Ortholog | |
Description | E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 |
Bud | 0 | 1 | 7 | 4 | 5 | 9 | 4 | 11 | 14 | – | 1 | 9 | 0 | 3 | 3 | 0 | 1 | 1 | 6 | 5 | 7 | 5 | 6 | 8 |
Bud Neck | 0 | 1 | 1 | 9 | 9 | 4 | 1 | 10 | 4 | – | 2 | 8 | 3 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 2 |
Bud Site | 1 | 0 | 5 | 9 | 6 | 13 | 21 | 27 | 12 | – | 14 | 19 | 1 | 1 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 10 | 3 | 27 | 6 | 8 | 15 | 14 | 13 | 7 | – | 12 | 12 | 18 | 31 | 36 | 22 | 19 | 30 | 0 | 0 | 3 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 4 | 0 | 0 | 2 | 0 | 1 | 0 |
Endosome | 1 | 1 | 4 | 0 | 10 | 2 | 4 | 3 | 1 | – | 4 | 1 | 6 | 2 | 7 | 5 | 8 | 4 | 9 | 5 | 23 | 5 | 17 | 19 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 1 | 0 | 1 | 3 | 3 | 1 |
Mitochondria | 1 | 22 | 92 | 48 | 39 | 97 | 130 | 141 | 109 | – | 92 | 121 | 17 | 3 | 13 | 24 | 11 | 66 | 1 | 1 | 9 | 2 | 2 | 4 |
Nucleus | 123 | 72 | 115 | 33 | 28 | 39 | 45 | 63 | 34 | – | 37 | 39 | 68 | 39 | 36 | 149 | 114 | 139 | 72 | 60 | 79 | 84 | 60 | 65 |
Nuclear Periphery | 14 | 7 | 8 | 0 | 2 | 3 | 5 | 6 | 2 | – | 2 | 6 | 2 | 0 | 1 | 11 | 10 | 8 | 5 | 0 | 6 | 0 | 2 | 0 |
Nucleolus | 86 | 105 | 148 | 175 | 191 | 201 | 283 | 334 | 181 | – | 165 | 197 | 196 | 170 | 211 | 219 | 179 | 267 | 151 | 95 | 149 | 122 | 178 | 226 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | – | 1 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 2 | 4 | 31 | 74 | 84 | 70 | 93 | 110 | 56 | – | 56 | 56 | 18 | 46 | 50 | 17 | 14 | 13 | 5 | 5 | 24 | 11 | 23 | 33 |
Vac/Vac Membrane | 7 | 4 | 7 | 1 | 2 | 1 | 2 | 4 | 1 | – | 0 | 2 | 4 | 2 | 1 | 11 | 19 | 10 | 8 | 4 | 18 | 15 | 20 | 24 |
Unique Cell Count | 221 | 167 | 315 | 223 | 250 | 299 | 392 | 453 | 266 | 233 | 284 | 260 | 227 | 277 | 390 | 320 | 411 | 266 | 183 | 340 | 257 | 324 | 393 | |
Labelled Cell Count | 247 | 220 | 446 | 360 | 384 | 454 | 607 | 723 | 425 | 386 | 470 | 333 | 302 | 363 | 463 | 382 | 543 | 266 | 183 | 340 | 257 | 324 | 393 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.0 | 7.5 | 5.2 | 5.5 | 5.8 | 5.5 | 5.3 | 5.0 | 4.6 | – | 4.5 | 4.4 | 7.6 | 7.9 | 7.2 | 11.1 | 12.5 | 10.9 | 7.6 | 8.4 | 9.0 |
Std Deviation (1e-4) | 2.4 | 2.6 | 1.4 | 2.3 | 1.5 | 1.9 | 2.1 | 1.5 | 1.4 | – | 1.7 | 2.0 | 2.9 | 2.6 | 2.4 | 3.8 | 4.4 | 3.5 | 2.3 | 2.5 | 2.9 |
Intensity Change (Log2) | – | – | – | 0.08 | 0.17 | 0.09 | 0.04 | -0.05 | -0.17 | – | -0.21 | -0.22 | 0.56 | 0.62 | 0.48 | 1.11 | 1.27 | 1.08 | 0.55 | 0.71 | 0.81 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | -0.2 | 0.6 | 0 | 0.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 1.8 | 0 | 2.0 | 2.6 | 3.0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.8 | -2.6 | -1.7 | -2.8 | -3.5 | -3.0 | – | -1.5 | -2.2 | -0.7 | 1.9 | 1.7 | -1.5 | -1.3 | -0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 2.1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.1 | 0 | 1.1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | -2.0 | -3.8 | 0.9 | 1.1 | 0.6 | 0 | – | 0 | 0 | -6.9 | -8.4 | -7.8 | -8.2 | -8.8 | -4.3 |
Nucleus | -5.6 | -6.9 | -6.7 | -7.9 | -7.3 | -6.5 | – | -5.3 | -6.4 | -2.7 | -4.9 | -6.5 | 0.5 | -0.2 | -0.8 |
Nuclear Periphery | 0 | 0 | -1.4 | -1.2 | -1.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.2 | 0.4 | -0.5 |
Nucleolus | 7.3 | 7.1 | 5.1 | 6.8 | 7.5 | 0 | – | 0 | 0 | 6.9 | 6.5 | 7.3 | 2.4 | 2.3 | 4.9 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 6.7 | 7.0 | 4.5 | 4.8 | 5.1 | 0 | – | 0 | 0 | -1.2 | 3.4 | 2.9 | -2.9 | -2.7 | -3.7 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0.5 | 2.4 | 0.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.0466 | 12.5861 | 10.8619 | 9.9179 | 9.6491 | 11.041 | 4.1655 | 6.9717 | 6.2322 | 4.8317 | 6.1118 | 5.5327 | 11.7122 | 14.1871 | 12.4253 | 11.6614 | 11.7071 | 12.0773 |
Actin | 0.0344 | 0 | 0.0081 | 0 | 0.004 | 0.0002 | 0.0057 | 0 | 0.0004 | 0.0004 | 0.0013 | 0.0023 | 0.0066 | 0 | 0 | 0 | 0 | 0 |
Bud | 0.0002 | 0 | 0.0004 | 0.0016 | 0.0009 | 0 | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0011 | 0.0002 | 0.0007 | 0.0049 | 0.0263 | 0.0003 | 0.0012 | 0.0002 | 0.0008 | 0.0007 | 0.0003 | 0.0002 | 0.0003 | 0.0003 | 0.0005 | 0.0011 | 0.0005 | 0.0002 |
Bud Periphery | 0.0004 | 0.0001 | 0.0009 | 0.0013 | 0.0019 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0002 | 0.0007 | 0.0003 | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0002 | 0 |
Bud Site | 0.0018 | 0.0002 | 0.0045 | 0.0012 | 0.0032 | 0.0001 | 0.0108 | 0.0002 | 0.0034 | 0.0002 | 0.0004 | 0.0002 | 0.0024 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0001 |
Cell Periphery | 0.0005 | 0.0004 | 0.0007 | 0.0019 | 0.0004 | 0.0002 | 0.0007 | 0.0006 | 0.0003 | 0.0003 | 0.0002 | 0.001 | 0.0004 | 0.0003 | 0.0007 | 0.0004 | 0.0003 | 0.0001 |
Cytoplasm | 0.0014 | 0 | 0.0018 | 0 | 0.0016 | 0 | 0.0022 | 0 | 0.0008 | 0.0011 | 0.002 | 0.0004 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0058 | 0.0001 | 0.0015 | 0.0005 | 0.0016 | 0.0028 | 0.0314 | 0.0028 | 0.0085 | 0.0038 | 0.0018 | 0.0049 | 0.0018 | 0.0001 | 0.0002 | 0.003 | 0.0003 | 0.0004 |
Eisosomes | 0.0003 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0008 | 0 | 0.0012 | 0 | 0.0018 | 0.0001 | 0.0019 | 0 | 0.0002 | 0.0003 | 0.0051 | 0.0011 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0.0037 | 0 | 0.0125 | 0.0001 | 0.0242 | 0.0006 | 0.0141 | 0.0004 | 0.0025 | 0.0046 | 0.057 | 0.013 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Golgi | 0.0031 | 0 | 0.0033 | 0 | 0.0034 | 0.0005 | 0.0041 | 0.0001 | 0.0006 | 0.0047 | 0.0033 | 0.0051 | 0.0004 | 0 | 0 | 0.0002 | 0 | 0 |
Lipid Particles | 0.0147 | 0.0009 | 0.0062 | 0.0008 | 0.0023 | 0.0044 | 0.03 | 0.002 | 0.0061 | 0.0228 | 0.0028 | 0.0342 | 0.0041 | 0.0004 | 0.0007 | 0.0087 | 0.0012 | 0.0012 |
Mitochondria | 0.0045 | 0.0002 | 0.0227 | 0.0002 | 0.0032 | 0.0091 | 0.0035 | 0.0009 | 0.0007 | 0.007 | 0.0034 | 0.0067 | 0.0011 | 0.0001 | 0.0001 | 0.0014 | 0.0007 | 0 |
None | 0.003 | 0 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0011 | 0 | 0.0005 | 0.0002 | 0.0011 | 0.0006 | 0.0004 | 0 | 0 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0019 | 0.0002 | 0.0027 | 0 | 0.0038 | 0.0002 | 0.0042 | 0.0001 | 0.0005 | 0.0046 | 0.0255 | 0.0318 | 0.0002 | 0.0002 | 0.0036 | 0.0002 | 0.0002 | 0 |
Nucleolus | 0.7635 | 0.8261 | 0.8404 | 0.8469 | 0.7701 | 0.8539 | 0.7659 | 0.8412 | 0.8549 | 0.7887 | 0.8011 | 0.7912 | 0.8248 | 0.8468 | 0.8852 | 0.8889 | 0.8573 | 0.8696 |
Nucleus | 0.1313 | 0.1694 | 0.0834 | 0.1315 | 0.1257 | 0.1253 | 0.0997 | 0.1391 | 0.1154 | 0.1529 | 0.0721 | 0.0916 | 0.1517 | 0.1463 | 0.1041 | 0.09 | 0.133 | 0.1269 |
Peroxisomes | 0.017 | 0 | 0.0008 | 0.0002 | 0.0005 | 0.0002 | 0.0032 | 0.0002 | 0.0006 | 0.0033 | 0.0002 | 0.0014 | 0.0009 | 0 | 0 | 0.0006 | 0.0002 | 0 |
Punctate Nuclear | 0.0043 | 0.0004 | 0.0006 | 0.0022 | 0.0069 | 0.0008 | 0.0133 | 0.0003 | 0.001 | 0.0018 | 0.0016 | 0.0051 | 0.0019 | 0.0045 | 0.0006 | 0.0017 | 0.0018 | 0.0012 |
Vacuole | 0.0033 | 0.0011 | 0.0044 | 0.0057 | 0.0138 | 0.0005 | 0.0038 | 0.0081 | 0.0016 | 0.0016 | 0.0088 | 0.0033 | 0.0013 | 0.0007 | 0.0022 | 0.0013 | 0.0018 | 0.0001 |
Vacuole Periphery | 0.0027 | 0.0005 | 0.0025 | 0.0009 | 0.0039 | 0.0006 | 0.0017 | 0.0035 | 0.001 | 0.0006 | 0.0107 | 0.0054 | 0.0006 | 0.0001 | 0.0008 | 0.0018 | 0.0023 | 0 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 47.6488 | 35.2026 | 47.3178 | 63.9698 | 44.4194 | 42.7309 | 50.2628 | 64.0386 | 62.1618 | 65.033 |
Translational Efficiency | 0.4647 | 0.4397 | 0.492 | 0.3728 | 0.4065 | 0.4734 | 0.4122 | 0.2845 | 0.3238 | 0.3333 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1150 | 406 | 331 | 1397 | 1622 | 1467 | 372 | 1312 | 2772 | 1873 | 703 | 2709 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 535.95 | 1034.26 | 1167.25 | 845.90 | 805.24 | 858.38 | 1012.64 | 834.77 | 693.52 | 896.50 | 1085.44 | 840.51 |
Standard Deviation | 69.81 | 200.29 | 141.63 | 114.97 | 148.21 | 146.01 | 135.21 | 105.86 | 180.22 | 175.06 | 158.35 | 110.79 |
Intensity Change Log 2 | — | 0.948429 | 1.122943 | 0.658389 | — | 0.092198 | 0.330631 | 0.051960 | — | 0.496886 | 0.700742 | 0.325523 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000258 | 0.000288 | 0.000421 | 0.000454 | 0.000530 | 0.000833 | 0.000335 | 0.000425 | 0.000417 | 0.000715 | 0.000376 | 0.000440 |
Bud Neck | 0.022768 | 0.003430 | 0.006084 | 0.025666 | 0.004413 | 0.011496 | 0.007112 | 0.014035 | 0.012027 | 0.009748 | 0.006628 | 0.020033 |
Bud Site | 0.022021 | 0.000674 | 0.000795 | 0.004010 | 0.004212 | 0.013757 | 0.000802 | 0.001584 | 0.011600 | 0.010921 | 0.000799 | 0.002835 |
Cell Periphery | 0.000672 | 0.000034 | 0.000012 | 0.000069 | 0.000223 | 0.000275 | 0.000029 | 0.000058 | 0.000410 | 0.000223 | 0.000021 | 0.000064 |
Cytoplasm | 0.215792* | 0.000227 | 0.000019 | 0.006134 | 0.006228 | 0.021094 | 0.000461 | 0.001993 | 0.093168 | 0.016571 | 0.000253 | 0.004128 |
Cytoplasmic Foci | 0.125529 | 0.001653 | 0.000116 | 0.001101 | 0.003808 | 0.020671 | 0.000171 | 0.000850 | 0.054305 | 0.016549 | 0.000145 | 0.000979 |
Eisosomes | 0.000157 | 0.000097 | 0.000063 | 0.000047 | 0.000178 | 0.000188 | 0.000053 | 0.000044 | 0.000169 | 0.000169 | 0.000058 | 0.000046 |
Endoplasmic Reticulum | 0.002889 | 0.000684 | 0.000908 | 0.002967 | 0.002828 | 0.003847 | 0.001136 | 0.003141 | 0.002854 | 0.003161 | 0.001029 | 0.003051 |
Endosome | 0.024403 | 0.000377 | 0.000088 | 0.002691 | 0.003958 | 0.018991 | 0.000431 | 0.001413 | 0.012440 | 0.014956 | 0.000269 | 0.002072 |
Golgi | 0.017435 | 0.000300 | 0.000287 | 0.001163 | 0.002451 | 0.015421 | 0.000265 | 0.000552 | 0.008667 | 0.012143 | 0.000275 | 0.000867 |
Lipid Particles | 0.019180 | 0.000090 | 0.000051 | 0.000363 | 0.000406 | 0.001617 | 0.000105 | 0.000239 | 0.008194 | 0.001286 | 0.000080 | 0.000303 |
Mitochondria | 0.011498 | 0.000770 | 0.000671 | 0.004707 | 0.002113 | 0.007102 | 0.000700 | 0.002177 | 0.006006 | 0.005730 | 0.000686 | 0.003482 |
Mitotic Spindle | 0.004352 | 0.000343 | 0.002573 | 0.023610 | 0.001042 | 0.006224 | 0.001912 | 0.006275 | 0.002415 | 0.004949 | 0.002223 | 0.015215 |
None | 0.197244* | 0.001005 | 0.000278 | 0.002744 | 0.007065 | 0.008559 | 0.000867 | 0.002000 | 0.085963 | 0.006922 | 0.000589 | 0.002383 |
Nuclear Periphery | 0.002068 | 0.000096 | 0.000107 | 0.003831 | 0.000160 | 0.000443 | 0.000275 | 0.002829 | 0.000952 | 0.000368 | 0.000196 | 0.003346 |
Nuclear Periphery Foci | 0.018970 | 0.000939 | 0.000344 | 0.004139 | 0.005630 | 0.007867 | 0.000723 | 0.003930 | 0.011164 | 0.006365 | 0.000545 | 0.004038 |
Nucleolus | 0.111764 | 0.519599* | 0.615262* | 0.361550* | 0.531704* | 0.430460* | 0.518888* | 0.393589* | 0.357487* | 0.449782* | 0.564265* | 0.377067* |
Nucleus | 0.176773* | 0.460097* | 0.361742* | 0.541984* | 0.408173* | 0.384394* | 0.459883* | 0.557871* | 0.312173* | 0.400804* | 0.413674* | 0.549678* |
Peroxisomes | 0.008630 | 0.007407 | 0.008954 | 0.000925 | 0.009028 | 0.016591 | 0.003576 | 0.000761 | 0.008863 | 0.014600 | 0.006108 | 0.000846 |
Vacuole | 0.013671 | 0.000918 | 0.000783 | 0.007348 | 0.004223 | 0.020852 | 0.001815 | 0.003714 | 0.008142 | 0.016531 | 0.001329 | 0.005588 |
Vacuole Periphery | 0.003928 | 0.000973 | 0.000443 | 0.004498 | 0.001628 | 0.009316 | 0.000461 | 0.002520 | 0.002582 | 0.007508 | 0.000453 | 0.003540 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.62 | -4.71 | -5.67 | -4.71 | -0.74 | -2.88 | 2.03 | 0.80 | 4.70 | -2.34 | -4.02 | -0.20 | -1.69 | 3.25 | -2.11 |
Bud Neck | 10.46 | 7.75 | -4.37 | -19.30 | -15.21 | -11.41 | -3.51 | -10.34 | -3.52 | -5.37 | 2.11 | 3.98 | -10.49 | -14.36 | -14.84 |
Bud Site | 10.25 | 10.05 | 7.25 | -5.49 | -5.05 | -7.96 | 4.71 | 3.02 | 11.09 | -2.75 | -0.04 | 10.94 | 7.20 | 7.85 | -5.70 |
Cell Periphery | 13.47 | 14.05 | 12.60 | -4.07 | -8.53 | -1.71 | 7.31 | 6.25 | 10.42 | -2.81 | 5.94 | 15.36 | 13.49 | 9.66 | -6.87 |
Cytoplasm | 34.10 | 34.13 | 32.18 | -6.49 | -6.68 | -5.50 | 4.34 | 2.64 | 7.77 | -3.59 | 19.49 | 27.60 | 25.49 | 5.79 | -7.48 |
Cytoplasmic Foci | 24.31 | 24.90 | 24.66 | 0.54 | -3.09 | -8.96 | 5.33 | 3.97 | 11.10 | -2.28 | 14.03 | 22.42 | 22.01 | 10.96 | -3.79 |
Eisosomes | 4.93 | 8.45 | 10.05 | 6.72 | 4.47 | -0.93 | 15.97 | 17.95 | 14.99 | 2.40 | 0.12 | 17.15 | 19.61 | 15.84 | 4.65 |
Endoplasmic Reticulum | 6.74 | 5.23 | -1.53 | -13.65 | -10.45 | -3.66 | 6.52 | -1.51 | 2.04 | -8.05 | -1.72 | 8.43 | -2.15 | -0.30 | -12.85 |
Endosome | 13.82 | 14.15 | 12.22 | -5.75 | -7.97 | -8.37 | 3.59 | 2.79 | 10.50 | -1.73 | -1.67 | 13.40 | 11.30 | 9.66 | -5.81 |
Golgi | 11.64 | 11.66 | 10.83 | -2.80 | -2.92 | -11.28 | 6.35 | 4.95 | 13.25 | -1.26 | -3.37 | 12.76 | 11.47 | 12.58 | -3.13 |
Lipid Particles | 11.31 | 11.34 | 11.12 | -3.51 | -5.19 | -4.42 | 2.85 | 1.64 | 5.00 | -1.81 | 9.23 | 11.18 | 10.85 | 4.40 | -4.81 |
Mitochondria | 8.46 | 8.46 | 4.51 | -4.56 | -4.56 | -6.13 | 5.04 | -0.55 | 6.00 | -6.73 | 0.30 | 9.46 | 3.52 | 2.97 | -6.10 |
Mitotic Spindle | 4.11 | 0.97 | -7.94 | -9.54 | -8.40 | -5.24 | -1.25 | -4.05 | -0.43 | -1.89 | -2.91 | -0.63 | -9.09 | -7.37 | -8.07 |
None | 24.76* | 24.86 | 24.49* | -6.91 | -11.46 | -1.27 | 6.74 | 5.50 | 7.63 | -2.97 | 20.62 | 22.63 | 22.09 | 6.53 | -8.36 |
Nuclear Periphery | 5.64 | 5.58 | -2.54 | -5.41 | -5.38 | -4.47 | -1.92 | -4.31 | -3.85 | -4.01 | 3.66 | 4.63 | -5.10 | -6.38 | -6.67 |
Nuclear Periphery Foci | 10.90 | 11.85 | 8.20 | -4.43 | -6.27 | -2.07 | 4.70 | 0.96 | 3.12 | -3.41 | 4.96 | 12.12 | 6.55 | 2.16 | -6.46 |
Nucleolus | -29.40* | -36.66* | -27.86* | 11.40 | 17.78 | 11.43 | 1.07 | 15.50 | 4.01 | 9.00 | -11.00 | -18.99 | -1.97 | 10.01 | 18.48 |
Nucleus | -20.42 | -14.49 | -37.52 | -4.59 | -11.28 | 2.86 | -3.70 | -16.36 | -18.44 | -7.09 | -11.76 | -10.68 | -33.40 | -18.49 | -12.54 |
Peroxisomes | 1.69 | -0.52 | 10.48 | 7.88 | 10.72 | -6.81 | 8.05 | 14.65 | 15.89 | 7.28 | -6.18 | 4.17 | 17.66 | 16.91 | 12.41 |
Vacuole | 12.71 | 13.19 | 2.73 | -6.28 | -6.37 | -12.07 | 0.62 | -0.79 | 10.30 | -0.99 | -7.63 | 5.87 | 1.00 | 7.48 | -4.25 |
Vacuole Periphery | 4.18 | 5.32 | -0.49 | -3.98 | -4.82 | -10.36 | 6.82 | -1.78 | 7.48 | -3.96 | -7.99 | 7.44 | -1.72 | 5.60 | -6.20 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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