Standard name | |
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Human Ortholog | |
Description | Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle; HEH2 has a paralog, SRC1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 4 | 3 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 3 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 6 | 8 | 0 | 4 | 8 | 7 | 14 | 15 | 8 | 6 | 15 | 8 | 23 | 56 | 115 | 27 | 12 | 20 | 1 | 1 | 0 | 4 | 2 | 4 |
Endoplasmic Reticulum | 1 | 1 | 0 | 5 | 0 | 1 | 0 | 1 | 3 | 1 | 2 | 7 | 0 | 0 | 3 | 8 | 14 | 9 | 1 | 1 | 0 | 0 | 2 | 4 |
Endosome | 14 | 24 | 3 | 1 | 7 | 11 | 33 | 19 | 6 | 16 | 8 | 4 | 19 | 43 | 48 | 36 | 48 | 34 | 8 | 15 | 4 | 17 | 82 | 124 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 0 | 2 | 6 | 11 |
Mitochondria | 135 | 38 | 0 | 13 | 9 | 36 | 50 | 60 | 100 | 88 | 126 | 106 | 10 | 29 | 7 | 52 | 22 | 18 | 11 | 13 | 7 | 25 | 73 | 115 |
Nucleus | 29 | 18 | 7 | 7 | 12 | 17 | 28 | 46 | 27 | 53 | 29 | 52 | 21 | 38 | 39 | 21 | 17 | 16 | 1 | 6 | 0 | 8 | 9 | 13 |
Nuclear Periphery | 180 | 249 | 27 | 61 | 77 | 94 | 147 | 152 | 103 | 127 | 117 | 165 | 254 | 315 | 374 | 116 | 93 | 62 | 367 | 358 | 83 | 211 | 310 | 276 |
Nucleolus | 15 | 6 | 1 | 3 | 7 | 21 | 32 | 21 | 30 | 24 | 36 | 29 | 24 | 46 | 20 | 35 | 21 | 19 | 0 | 3 | 0 | 4 | 12 | 13 |
Peroxisomes | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 4 |
SpindlePole | 0 | 2 | 2 | 0 | 2 | 3 | 3 | 8 | 2 | 6 | 2 | 5 | 3 | 18 | 21 | 14 | 11 | 5 | 2 | 3 | 0 | 2 | 13 | 11 |
Vac/Vac Membrane | 109 | 208 | 37 | 24 | 63 | 48 | 79 | 57 | 25 | 54 | 31 | 59 | 92 | 131 | 288 | 135 | 129 | 106 | 5 | 13 | 3 | 14 | 47 | 41 |
Unique Cell Count | 346 | 385 | 57 | 90 | 143 | 171 | 291 | 314 | 220 | 292 | 252 | 326 | 351 | 509 | 683 | 282 | 251 | 194 | 409 | 425 | 105 | 296 | 578 | 636 |
Labelled Cell Count | 489 | 555 | 77 | 118 | 186 | 239 | 387 | 384 | 305 | 375 | 368 | 438 | 446 | 679 | 921 | 448 | 372 | 294 | 409 | 425 | 105 | 296 | 578 | 636 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 7.4 | 7.5 | 7.1 | 8.0 | 6.6 | 6.7 | 6.7 | 5.6 | 6.1 | 5.6 | 6.1 | 8.1 | 7.6 | 7.7 | 8.1 | 9.1 | 7.8 | 7.6 | 7.5 | 7.8 |
Std Deviation (1e-4) | 1.0 | 1.1 | 1.2 | 1.6 | 1.7 | 1.4 | 1.5 | 1.4 | 1.3 | 1.4 | 1.5 | 1.7 | 1.4 | 1.6 | 1.8 | 2.0 | 6.4 | 2.0 | 1.3 | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.08 | 0.1 | -0.18 | -0.15 | -0.15 | -0.41 | -0.29 | -0.42 | -0.3 | 0.11 | 0.03 | 0.05 | 0.12 | 0.28 | 0.06 | 0.03 | 0.0 | 0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 3.4 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 1.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.6 | 2.5 | 2.3 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.0 | 0 | 3.8 | 3.4 | 3.6 | 6.4 | 4.8 | 6.9 | 5.1 | 0 | 0 | 0 | 3.5 | 0 | 0 |
Nucleus | -0.9 | -0.8 | -0.5 | -0.6 | 0.5 | 0 | 1.1 | -0.2 | 0.7 | 0 | 0 | 0 | 0 | 0 | -0.9 |
Nuclear Periphery | 2.5 | 0.8 | 1.0 | 0.4 | 0.1 | -0.1 | -0.5 | -0.1 | 0.5 | 3.8 | 2.1 | 1.1 | -0.9 | -1.4 | -2.1 |
Nucleolus | 0 | 0 | 2.3 | 2.2 | 0 | 2.5 | 0 | 2.6 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.6 | -2.7 | -5.0 | -5.5 | -7.5 | -8.6 | -7.3 | -8.7 | -7.5 | -5.8 | -6.1 | -3.3 | -2.3 | -1.8 | -1.4 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.6435 | 3.3391 | 3.2198 | 2.8654 | 2.8421 | 3.3394 | 2.7398 | 2.9052 | 2.9544 | 2.6845 | 2.1794 | 2.6052 | 2.465 | 3.2718 | 2.4309 | 2.2671 | 2.2238 | 2.2651 |
Actin | 0.0148 | 0.0004 | 0.0092 | 0.0015 | 0.0151 | 0.0018 | 0.0044 | 0.0005 | 0.008 | 0.0011 | 0.0023 | 0.0005 | 0.0017 | 0.001 | 0.0051 | 0.0018 | 0.0008 | 0.0008 |
Bud | 0.0003 | 0.0001 | 0.0004 | 0.0008 | 0.0003 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0007 | 0.0001 | 0.0003 | 0.0007 | 0.0015 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 |
Bud Periphery | 0.0007 | 0.0002 | 0.0013 | 0.0008 | 0.0005 | 0.0003 | 0.0006 | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0001 |
Bud Site | 0.0009 | 0.0003 | 0.0028 | 0.0296 | 0.0017 | 0.0001 | 0.0017 | 0 | 0.0006 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Cell Periphery | 0.0002 | 0 | 0.0016 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0013 | 0.0038 | 0.0109 | 0.0011 | 0.0007 | 0.0001 | 0.0035 | 0.0002 | 0.0018 | 0.0001 | 0.0003 | 0.0002 | 0.0009 | 0.0003 | 0.0003 | 0.0003 | 0.0001 | 0.0002 |
Cytoplasmic Foci | 0.0091 | 0.0005 | 0.003 | 0.0022 | 0.0099 | 0.0026 | 0.0195 | 0.0005 | 0.0126 | 0.0002 | 0.0013 | 0.0003 | 0.0005 | 0.0003 | 0.0014 | 0.0007 | 0.0001 | 0.0005 |
Eisosomes | 0.0001 | 0 | 0.0009 | 0 | 0.0002 | 0 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0041 | 0.0034 | 0.0072 | 0.002 | 0.0052 | 0.0008 | 0.0024 | 0.004 | 0.0031 | 0.0026 | 0.0025 | 0.0012 | 0.0031 | 0.0059 | 0.0042 | 0.0043 | 0.0016 | 0.0022 |
Endosome | 0.0218 | 0.0083 | 0.0062 | 0.0031 | 0.0481 | 0.0066 | 0.0146 | 0.0016 | 0.0143 | 0.0026 | 0.0081 | 0.0035 | 0.0062 | 0.0028 | 0.0027 | 0.0019 | 0.0018 | 0.0016 |
Golgi | 0.0054 | 0.0011 | 0.0033 | 0.0009 | 0.0126 | 0.0025 | 0.0045 | 0.0008 | 0.0089 | 0.0009 | 0.0055 | 0.0008 | 0.0019 | 0.0007 | 0.0006 | 0.0007 | 0.0006 | 0.0004 |
Lipid Particles | 0.0345 | 0.0069 | 0.0055 | 0.0058 | 0.0268 | 0.0063 | 0.0239 | 0.0106 | 0.0232 | 0.0086 | 0.0402 | 0.0022 | 0.0198 | 0.0053 | 0.0036 | 0.0211 | 0.0042 | 0.0023 |
Mitochondria | 0.0076 | 0.0027 | 0.0087 | 0.0051 | 0.0132 | 0.0057 | 0.0075 | 0.011 | 0.0224 | 0.0057 | 0.0438 | 0.0145 | 0.0089 | 0.0039 | 0.0029 | 0.0034 | 0.0024 | 0.0031 |
None | 0.0018 | 0.0003 | 0.0014 | 0.0006 | 0.0013 | 0.0002 | 0.0079 | 0.0002 | 0.0028 | 0.0006 | 0.0004 | 0.0002 | 0.0028 | 0.0004 | 0.0013 | 0.0005 | 0.0004 | 0.0002 |
Nuclear Periphery | 0.8282 | 0.882 | 0.8694 | 0.8997 | 0.8102 | 0.9416 | 0.8319 | 0.9003 | 0.8235 | 0.9184 | 0.8411 | 0.9428 | 0.887 | 0.9082 | 0.9023 | 0.8625 | 0.9502 | 0.9543 |
Nucleolus | 0.0037 | 0.0033 | 0.0006 | 0.0011 | 0.0008 | 0.0007 | 0.0038 | 0.0008 | 0.0005 | 0.0013 | 0.001 | 0.0023 | 0.0013 | 0.0012 | 0.001 | 0.0022 | 0.0018 | 0.0005 |
Nucleus | 0.0129 | 0.0483 | 0.0175 | 0.0093 | 0.0078 | 0.0038 | 0.0138 | 0.0075 | 0.008 | 0.0075 | 0.0216 | 0.0076 | 0.0136 | 0.0146 | 0.0081 | 0.0416 | 0.0064 | 0.0089 |
Peroxisomes | 0.0078 | 0.0002 | 0.0063 | 0.0007 | 0.0188 | 0.0069 | 0.0102 | 0.0003 | 0.0077 | 0.0002 | 0.0016 | 0.0001 | 0.0005 | 0.0002 | 0.0027 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0116 | 0.0016 | 0.0022 | 0.0025 | 0.0048 | 0.0017 | 0.0101 | 0.0084 | 0.0022 | 0.0016 | 0.0026 | 0.0007 | 0.0034 | 0.0024 | 0.0045 | 0.0093 | 0.0034 | 0.004 |
Vacuole | 0.0041 | 0.0035 | 0.0032 | 0.0016 | 0.0025 | 0.0008 | 0.0027 | 0.0013 | 0.0027 | 0.0013 | 0.002 | 0.0009 | 0.0021 | 0.0021 | 0.0014 | 0.0008 | 0.0007 | 0.0003 |
Vacuole Periphery | 0.0285 | 0.0332 | 0.0378 | 0.0307 | 0.018 | 0.0172 | 0.0357 | 0.0519 | 0.0567 | 0.0465 | 0.0249 | 0.0214 | 0.0452 | 0.0503 | 0.0575 | 0.0484 | 0.0252 | 0.0203 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 8.5857 | 11.8841 | 18.3718 | 14.7801 | 11.4909 | 12.1794 | 16.7723 | 18.4164 | 18.168 | 15.9614 |
Translational Efficiency | 1.6272 | 1.3004 | 0.9278 | 1.316 | 1.2168 | 1.3815 | 0.9545 | 1.0337 | 1.0611 | 1.0361 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1007 | 1386 | 839 | 1239 | 1961 | 2359 | 383 | 349 | 2968 | 3745 | 1222 | 1588 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 661.51 | 845.03 | 1012.26 | 992.68 | 815.49 | 819.82 | 1090.57 | 1039.70 | 763.25 | 829.15 | 1036.80 | 1003.01 |
Standard Deviation | 85.51 | 137.15 | 120.95 | 140.68 | 104.47 | 118.37 | 129.84 | 140.15 | 122.50 | 126.24 | 129.03 | 141.91 |
Intensity Change Log 2 | — | 0.353240 | 0.613745 | 0.585566 | — | 0.007640 | 0.419343 | 0.350428 | — | 0.172722 | 0.509662 | 0.460500 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000251 | 0.000140 | 0.000425 | 0.000372 | 0.000079 | 0.000153 | 0.000125 | 0.000209 | 0.000137 | 0.000148 | 0.000331 | 0.000336 |
Bud Neck | 0.000322 | 0.000373 | 0.000922 | 0.006159 | 0.000068 | 0.000444 | 0.000698 | 0.006149 | 0.000154 | 0.000418 | 0.000852 | 0.006157 |
Bud Site | 0.000183 | 0.000155 | 0.000186 | 0.003797 | 0.000022 | 0.000281 | 0.000195 | 0.002691 | 0.000077 | 0.000234 | 0.000189 | 0.003554 |
Cell Periphery | 0.000070 | 0.000025 | 0.000031 | 0.000071 | 0.000021 | 0.000050 | 0.000010 | 0.000061 | 0.000038 | 0.000041 | 0.000025 | 0.000069 |
Cytoplasm | 0.008097 | 0.002141 | 0.001864 | 0.002806 | 0.000455 | 0.004959 | 0.000145 | 0.005465 | 0.003048 | 0.003916 | 0.001325 | 0.003390 |
Cytoplasmic Foci | 0.007159 | 0.001632 | 0.000166 | 0.009227 | 0.000860 | 0.001840 | 0.000191 | 0.013871 | 0.002997 | 0.001763 | 0.000174 | 0.010248 |
Eisosomes | 0.000020 | 0.000007 | 0.000011 | 0.000010 | 0.000004 | 0.000010 | 0.000002 | 0.000010 | 0.000010 | 0.000009 | 0.000008 | 0.000010 |
Endoplasmic Reticulum | 0.006008 | 0.004244 | 0.007191 | 0.002758 | 0.004402 | 0.006000 | 0.004657 | 0.003447 | 0.004947 | 0.005350 | 0.006397 | 0.002909 |
Endosome | 0.006242 | 0.003297 | 0.001053 | 0.014999 | 0.002371 | 0.005488 | 0.000818 | 0.022144 | 0.003684 | 0.004677 | 0.000979 | 0.016569 |
Golgi | 0.002990 | 0.000891 | 0.000081 | 0.006075 | 0.000510 | 0.000923 | 0.002592 | 0.002255 | 0.001351 | 0.000911 | 0.000868 | 0.005236 |
Lipid Particles | 0.100149* | 0.008664 | 0.000965 | 0.005096 | 0.022965 | 0.009768 | 0.000954 | 0.005023 | 0.049153 | 0.009359 | 0.000962 | 0.005080 |
Mitochondria | 0.015195 | 0.003605 | 0.001638 | 0.002034 | 0.003779 | 0.006622 | 0.000333 | 0.003580 | 0.007652 | 0.005506 | 0.001229 | 0.002374 |
Mitotic Spindle | 0.025514 | 0.025467 | 0.032241 | 0.271026* | 0.005688 | 0.011509 | 0.019329 | 0.222518* | 0.012415 | 0.016675 | 0.028194 | 0.260366* |
None | 0.011270 | 0.001304 | 0.002070 | 0.000918 | 0.000552 | 0.004076 | 0.000065 | 0.000514 | 0.004189 | 0.003050 | 0.001442 | 0.000829 |
Nuclear Periphery | 0.080677 | 0.165906* | 0.343728* | 0.154217* | 0.169301* | 0.227210* | 0.320588* | 0.198139* | 0.139232* | 0.204521* | 0.336475* | 0.163870* |
Nuclear Periphery Foci | 0.686007* | 0.739424* | 0.492345* | 0.469212* | 0.774856* | 0.635099* | 0.634323* | 0.473548* | 0.744711* | 0.673709* | 0.536844* | 0.470165* |
Nucleolus | 0.025903 | 0.016955 | 0.039207 | 0.015632 | 0.006077 | 0.026622 | 0.005054 | 0.009552 | 0.012804 | 0.023044 | 0.028503 | 0.014296 |
Nucleus | 0.020061 | 0.022610 | 0.074233 | 0.015616 | 0.005805 | 0.051201 | 0.008773 | 0.006991 | 0.010642 | 0.040620 | 0.053717 | 0.013721 |
Peroxisomes | 0.002291 | 0.000224 | 0.000306 | 0.001226 | 0.000388 | 0.000567 | 0.000138 | 0.000777 | 0.001034 | 0.000440 | 0.000253 | 0.001128 |
Vacuole | 0.000686 | 0.001506 | 0.001030 | 0.014170 | 0.000806 | 0.004096 | 0.000693 | 0.018918 | 0.000765 | 0.003138 | 0.000924 | 0.015214 |
Vacuole Periphery | 0.000904 | 0.001433 | 0.000306 | 0.004578 | 0.000991 | 0.003083 | 0.000317 | 0.004138 | 0.000961 | 0.002472 | 0.000309 | 0.004481 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 2.87 | -4.24 | -1.24 | -2.08 | 0.79 | -4.73 | -1.88 | -3.67 | -1.83 | -2.42 | -0.68 | -6.28 | -2.27 | -2.17 | 0.25 |
Bud Neck | -0.78 | -4.91 | -8.84 | -8.76 | -7.21 | -7.46 | -1.33 | -5.82 | -5.48 | -3.92 | -6.31 | -3.89 | -10.61 | -10.17 | -7.99 |
Bud Site | 0.38 | -2.20 | -6.70 | -6.73 | -6.32 | -2.42 | -1.04 | -5.41 | -4.79 | -3.88 | -2.22 | -2.43 | -7.93 | -7.51 | -7.07 |
Cell Periphery | 7.41 | 3.79 | -0.41 | -4.11 | -2.58 | -4.92 | 4.34 | -3.54 | -1.41 | -4.27 | -0.69 | 1.33 | -3.63 | -3.22 | -4.04 |
Cytoplasm | 3.41 | 3.59 | 2.02 | -2.48 | -3.00 | -7.40 | 2.09 | -1.91 | -0.31 | -2.02 | -1.45 | 2.33 | -1.93 | -0.94 | -3.82 |
Cytoplasmic Foci | 4.64 | 6.02 | -1.03 | -6.11 | -7.60 | -3.27 | 2.77 | -3.96 | -3.61 | -4.14 | 2.53 | 6.51 | -5.77 | -7.05 | -8.53 |
Eisosomes | 7.36 | 1.60 | 3.86 | -4.51 | 1.52 | -6.17 | 2.72 | -4.07 | -0.41 | -5.01 | 0.90 | -1.38 | -2.27 | -3.04 | -0.22 |
Endoplasmic Reticulum | 2.91 | -2.63 | 4.54 | 2.19 | 6.50 | -2.84 | -0.41 | 1.12 | 2.94 | 1.30 | -1.02 | -3.76 | 3.95 | 4.74 | 6.48 |
Endosome | 2.36 | 4.52 | -5.26 | -7.96 | -10.08 | -3.88 | 3.87 | -5.24 | -4.34 | -5.67 | -1.52 | 5.48 | -9.26 | -8.39 | -11.49 |
Golgi | 3.82 | 5.71 | -1.89 | -3.64 | -4.31 | -1.87 | -0.82 | -1.81 | -1.35 | 0.12 | 1.84 | 0.61 | -3.40 | -3.85 | -3.14 |
Lipid Particles | 15.01 | 16.54 | 15.76 | 2.44 | -4.80 | 6.07 | 11.28 | 7.99 | 3.46 | -3.87 | 15.31 | 19.24 | 17.06 | 4.47 | -5.92 |
Mitochondria | 5.88 | 7.04 | 6.77 | 2.65 | -1.02 | -2.99 | 5.04 | 0.14 | 1.46 | -1.52 | 2.32 | 7.95 | 5.53 | 4.49 | -2.09 |
Mitotic Spindle | -0.15 | -2.06 | -24.19 | -24.28* | -22.29 | -3.74 | -3.11 | -13.14 | -12.72 | -12.00 | -2.70 | -5.40 | -29.19 | -28.54* | -25.64 |
None | 5.55 | 4.77 | 5.75 | 1.12 | 1.74 | -5.41 | 4.88 | 0.05 | 5.09 | -1.79 | 1.70 | 3.42 | 5.15 | 4.70 | 1.34 |
Nuclear Periphery | -14.01* | -25.16 | -10.07 | 2.85 | 17.57 | -9.09 | -12.72 | -1.63 | 2.78 | 8.02 | -13.90 | -24.04 | -2.78 | 8.09 | 19.85 |
Nuclear Periphery Foci | -4.77 | 13.84 | 16.84 | 24.80* | 2.68 | 17.66* | 11.19 | 17.92 | 9.55 | 8.63 | 10.60 | 22.67 | 30.54 | 22.98* | 7.09 |
Nucleolus | 3.06 | -3.04 | 2.78 | -0.16 | 5.94 | -10.18 | 0.71 | -1.97 | 4.76 | -2.25 | -5.78 | -6.32 | -1.49 | 3.21 | 4.49 |
Nucleus | -0.80 | -9.70 | -0.46 | 0.31 | 9.33 | -13.87 | -1.05 | -1.33 | 12.27 | 0.11 | -12.58 | -11.04 | -3.70 | 7.45 | 8.53 |
Peroxisomes | 4.19 | 3.79 | 1.66 | -3.84 | -3.15 | -1.37 | 1.17 | -2.52 | -1.51 | -3.75 | 2.82 | 3.51 | -0.75 | -3.29 | -3.86 |
Vacuole | -2.12 | -3.01 | -8.47 | -8.01 | -7.74 | -5.18 | 0.33 | -4.90 | -4.08 | -4.91 | -5.56 | -2.61 | -9.74 | -8.39 | -9.23 |
Vacuole Periphery | -1.14 | 4.48 | -5.37 | -3.88 | -6.33 | -4.52 | 3.87 | -2.91 | -0.95 | -3.56 | -4.45 | 5.39 | -6.00 | -3.05 | -7.24 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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