Standard name
Human Ortholog
Description Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.05 0 0.05 0.05 0.06 0 0.06 0 0.08 0.16 0.14 0.06 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.14 0 0.05 0 0.06 0.22 0.34 0.19 0.43 0.32 0.46 0.25 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.93 0.95 0.91 0.93 0.89 0.84 0.86 0.8 0.85 0.8 0.87 0.91 0.81 0.83 0.86 0.87 0.87 0.86 0.85 0.83 0.78 0.66 0.6
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.21 0.1 0 0 0 0 0.07 0 0.08 0.08 0.09 0.09 0 0 0 0 0 0.06 0.05 0.06 0.08 0.11 0.17 0.15
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.05 0.09 0.11
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 3 1 2 2 1 0 0 0 0 0 0 0 0 0 1 1 6
Bud Neck 0 0 0 0 0 0 1 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 3 2 4 5 9 5 6 0 0 0 0 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0
Cytoplasm 3 5 4 21 15 18 26 24 23 13 22 5 30 57 51 16 13 16 0 1 0 0 2 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4 3 1 0 0 0 0 1 3
Endosome 0 0 0 2 0 0 1 0 0 0 0 0 2 0 2 1 2 1 3 0 1 1 6 10
Golgi 0 1 2 0 0 0 0 0 0 0 0 0 1 2 0 2 3 0 1 2 1 0 1 1
Mitochondria 21 4 10 11 18 92 177 99 159 135 177 81 2 1 0 10 8 10 1 1 2 3 4 11
Nucleus 143 142 209 271 275 381 441 451 294 358 308 276 340 282 298 232 217 248 134 140 196 186 218 281
Nuclear Periphery 2 0 0 0 1 2 1 2 3 3 1 2 1 1 0 3 4 0 0 0 0 0 0 4
Nucleolus 32 15 4 5 1 4 36 22 31 34 35 28 8 9 11 1 3 16 7 9 19 25 55 70
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0
SpindlePole 0 0 10 4 3 2 0 9 3 5 4 4 6 4 5 4 2 0 0 1 1 5 6 13
Vac/Vac Membrane 0 0 1 4 4 1 2 0 2 3 4 6 1 4 3 16 8 12 4 6 9 11 28 53
Unique Cell Count 153 152 219 297 297 427 528 522 368 422 383 319 374 350 358 269 249 284 156 165 237 239 330 466
Labelled Cell Count 201 167 240 318 317 504 688 616 522 562 558 409 391 361 373 289 264 306 156 165 237 239 330 466


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 7.6 6.2 6.0 5.9 4.9 4.7 5.4 4.6 4.9 4.6 5.1 6.7 7.0 6.9 9.5 9.3 8.6 6.7 7.0 7.5
Std Deviation (1e-4) 0.7 1.3 1.4 1.5 1.4 1.3 1.4 1.4 1.4 1.3 1.4 1.2 1.5 1.8 1.4 2.0 2.2 2.0 1.3 1.4 1.5
Intensity Change (Log2) – – – -0.04 -0.07 -0.33 -0.39 -0.21 -0.43 -0.34 -0.44 -0.29 0.11 0.19 0.16 0.62 0.59 0.48 0.12 0.18 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.7 1.9 1.6 2.0 1.8 2.5 0.9 2.3 0 3.1 5.4 4.9 2.3 2.0 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -0.5 0.7 5.6 0 5.0 0 0 0 6.3 0 0 0 -0.5 -0.8 -0.6
Nucleus -1.8 -1.3 -2.7 -4.4 -3.6 -5.2 -4.0 -5.1 -3.4 -2.0 -5.0 -4.4 -3.4 -3.1 -3.1
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1.6 0 0 0 3.3 0 0.6 0.9 0 0 2.2
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -2.2 -2.5 0 0 -2.2 0 0 0 -2.4 -2.1 -2.6 -2.3 -2.0 -2.6 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 3.3 0 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.9297 1.3655 1.3452 1.156 0.7055 0.9969 1.3043 1.5951 1.1674 1.17 1.494 1.4295 2.1565 1.9607 1.6836 1.7749 1.7315 1.9565
Actin 0.0259 0.0004 0.0266 0.0017 0.0392 0.0003 0.0026 0.0002 0 0.0023 0.0001 0.0012 0.0381 0 0.0175 0 0.002 0.0048
Bud 0.0006 0 0.0005 0.0001 0.0002 0 0.0011 0.0004 0 0.0002 0.0001 0.0001 0.001 0 0.0004 0 0.0002 0.0004
Bud Neck 0.0038 0.0001 0.0028 0.0001 0.002 0.0004 0.0018 0.0003 0.0001 0.0008 0.0005 0.0007 0.0036 0.0001 0.0008 0 0.0004 0.0008
Bud Periphery 0.001 0 0.0008 0.0001 0.0004 0.0001 0.0026 0.0005 0 0.0004 0.0002 0.0003 0.0014 0 0.0007 0 0.0005 0.0009
Bud Site 0.0093 0.0002 0.0078 0.0003 0.0004 0 0.0088 0.0026 0 0.0007 0.0001 0.0001 0.0042 0.0001 0.0021 0 0.0002 0.0004
Cell Periphery 0.0003 0 0.0002 0 0.0001 0 0.0005 0.0001 0 0.0002 0 0.0002 0.0004 0 0.0002 0 0 0.0001
Cytoplasm 0.0178 0.0001 0.0016 0.0015 0.0038 0.0001 0.0089 0.0003 0.0014 0.0051 0.0002 0.002 0.004 0.0001 0.0007 0 0.0002 0.0005
Cytoplasmic Foci 0.016 0.001 0.0059 0.0034 0.0009 0.0001 0.0084 0.0006 0.0005 0.0064 0 0.0012 0.0095 0 0.0032 0 0.0004 0.0006
Eisosomes 0.0006 0 0.0002 0 0.0008 0 0.0001 0 0 0.0001 0 0 0.0004 0 0.0002 0 0 0.0001
Endoplasmic Reticulum 0.0057 0 0.0018 0.0011 0.005 0.0005 0.0009 0 0.0001 0.0021 0.0001 0.0019 0.0028 0 0.001 0 0.0012 0.0008
Endosome 0.022 0.0005 0.0199 0.0223 0.0158 0.0025 0.0083 0.0007 0.0056 0.0381 0 0.0112 0.0126 0 0.0084 0 0.0135 0.0044
Golgi 0.0097 0.001 0.0082 0.0019 0.0041 0.0004 0.0024 0.0003 0.0011 0.0105 0 0.0022 0.004 0 0.004 0 0.0022 0.0009
Lipid Particles 0.027 0.001 0.0108 0.0041 0.0051 0.0013 0.0076 0.0001 0.0034 0.0243 0 0.0043 0.0074 0 0.0068 0 0.0024 0.0009
Mitochondria 0.0099 0.0009 0.0093 0.0007 0.0029 0.0004 0.0197 0.0008 0.0005 0.0343 0.0006 0.0044 0.0132 0.0001 0.0031 0.0001 0.0058 0.0049
None 0.0169 0 0.0016 0.0024 0.007 0.0001 0.0011 0 0.0097 0.0023 0.0001 0.0015 0.0068 0 0.0007 0 0.0006 0.0006
Nuclear Periphery 0.0359 0.0008 0.0099 0.0063 0.0633 0.0084 0.0059 0.0006 0.0003 0.0043 0.0045 0.0092 0.0169 0.001 0.0099 0.0009 0.0199 0.0099
Nucleolus 0.0262 0.0152 0.0147 0.0068 0.0535 0.041 0.0229 0.0208 0.0138 0.0103 0.0797 0.0615 0.021 0.0187 0.0111 0.0144 0.0516 0.043
Nucleus 0.7302 0.9655 0.8567 0.939 0.7737 0.9415 0.869 0.9693 0.9625 0.8416 0.8858 0.8748 0.8211 0.9774 0.9138 0.9842 0.8921 0.9175
Peroxisomes 0.0164 0.0019 0.0055 0.0008 0.001 0.0001 0.002 0.0006 0.0001 0.0086 0 0.0004 0.0063 0 0.0025 0 0.0004 0.0004
Punctate Nuclear 0.0195 0.0112 0.0118 0.0064 0.0149 0.0019 0.0187 0.0013 0.0003 0.0008 0.0276 0.0208 0.0194 0.0025 0.0104 0.0003 0.002 0.0058
Vacuole 0.0033 0.0001 0.0022 0.0006 0.0028 0.0003 0.0046 0.0003 0.0005 0.0043 0.0002 0.0013 0.0035 0 0.0015 0 0.0014 0.0012
Vacuole Periphery 0.0021 0 0.0013 0.0005 0.0032 0.0005 0.002 0.0001 0.0002 0.0023 0.0001 0.0008 0.0023 0 0.0011 0 0.0031 0.0013

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9935 8.1687 14.3962 16.7271 6.6643 12.5577 13.9378 19.8593 23.4608 15.3033
Translational Efficiency 2.3317 4.0557 2.4659 2.2556 3.9756 2.1685 2.2002 1.5088 1.4549 1.6949

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
69 353 161 1138 2031 2158 1996 1293 2100 2511 2157 2431

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 842.28 874.27 1044.73 1017.76 774.02 850.85 962.94 1024.39 776.26 854.14 969.04 1021.29
Standard Deviation 108.19 136.41 127.61 159.14 98.08 112.61 142.34 175.10 99.18 116.53 142.92 167.85
Intensity Change Log 2 — 0.053779 0.310758 0.273026 — 0.136534 0.315075 0.404322 — 0.094002 0.312827 0.337394

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000493 0.000854 0.001399 0.002125 0.000220 0.000466 0.000699 0.001050 0.000229 0.000521 0.000751 0.001553
Bud Neck 0.004722 0.013332 0.009246 0.033087 0.006803 0.014161 0.015603 0.026214 0.006735 0.014045 0.015128 0.029432
Bud Site 0.000331 0.006507 0.001420 0.006720 0.000520 0.002100 0.001085 0.005688 0.000513 0.002720 0.001110 0.006171
Cell Periphery 0.000038 0.000097 0.000072 0.000126 0.000053 0.000054 0.000080 0.000154 0.000052 0.000060 0.000080 0.000141
Cytoplasm 0.000298 0.002613 0.001650 0.001938 0.001376 0.001835 0.001927 0.006476 0.001341 0.001944 0.001906 0.004351
Cytoplasmic Foci 0.000062 0.000458 0.000107 0.001044 0.000270 0.000821 0.000199 0.002732 0.000264 0.000770 0.000192 0.001942
Eisosomes 0.000027 0.000030 0.000055 0.000032 0.000018 0.000028 0.000035 0.000036 0.000019 0.000028 0.000037 0.000035
Endoplasmic Reticulum 0.003415 0.004309 0.004658 0.003707 0.002070 0.003388 0.002719 0.003342 0.002114 0.003518 0.002864 0.003513
Endosome 0.000126 0.000920 0.000182 0.002186 0.000236 0.001028 0.000137 0.004158 0.000233 0.001013 0.000141 0.003235
Golgi 0.000067 0.000823 0.000149 0.001768 0.000107 0.000589 0.000088 0.002384 0.000106 0.000622 0.000092 0.002096
Lipid Particles 0.000147 0.001160 0.000191 0.000147 0.000113 0.000340 0.000076 0.000578 0.000114 0.000455 0.000084 0.000377
Mitochondria 0.000670 0.001394 0.001141 0.004875 0.000580 0.002752 0.000952 0.002826 0.000583 0.002561 0.000966 0.003785
Mitotic Spindle 0.000019 0.001491 0.003987 0.025320 0.001849 0.007078 0.001692 0.019794 0.001789 0.006293 0.001863 0.022381
None 0.001892 0.004358 0.003101 0.003027 0.002291 0.002173 0.006947 0.009422 0.002278 0.002480 0.006660 0.006428
Nuclear Periphery 0.000217 0.000174 0.000215 0.002739 0.000287 0.000883 0.000228 0.001497 0.000285 0.000783 0.000227 0.002078
Nuclear Periphery Foci 0.000073 0.001085 0.000164 0.001312 0.000200 0.000733 0.000110 0.001988 0.000195 0.000782 0.000114 0.001671
Nucleolus 0.029059 0.043789 0.033933 0.010584 0.038559 0.042822 0.027059 0.015778 0.038247 0.042958 0.027573 0.013347
Nucleus 0.957504* 0.913743* 0.935926* 0.891818* 0.943760* 0.915205* 0.939585* 0.886292* 0.944212* 0.915000* 0.939312* 0.888879*
Peroxisomes 0.000546 0.000704 0.000985 0.000318 0.000230 0.001017 0.000254 0.000657 0.000241 0.000973 0.000308 0.000498
Vacuole 0.000206 0.001554 0.001261 0.004861 0.000277 0.001637 0.000433 0.006310 0.000275 0.001626 0.000495 0.005632
Vacuole Periphery 0.000088 0.000606 0.000161 0.002264 0.000181 0.000888 0.000092 0.002625 0.000178 0.000849 0.000097 0.002456

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.47 -7.86 -2.68 -1.91 -0.37 -6.13 -19.92 -19.25 -12.33 -9.33 -7.88 -21.66 -5.75 -4.37 -3.13
Bud Neck -3.70 -3.47 -11.74 -7.14 -10.12 -7.74 -12.91 -14.72 -9.60 -6.89 -8.17 -13.02 -18.51 -12.43 -9.65
Bud Site -2.01 -6.81 -5.94 0.25 -4.10 -4.91 -7.17 -6.35 -3.65 -5.30 -4.46 -7.90 -8.48 -3.33 -7.06
Cell Periphery -2.51 -1.90 -4.29 -0.25 -1.54 -0.31 -2.19 -5.34 -5.66 -3.98 -0.94 -2.31 -5.49 -5.29 -3.94
Cytoplasm -2.18 -1.32 -4.15 0.01 -0.09 -1.16 -1.10 -4.20 -3.38 -3.89 -1.46 -1.29 -4.21 -2.87 -3.68
Cytoplasmic Foci -1.68 -1.04 -1.57 -0.35 -1.37 -1.77 0.74 -2.16 -0.94 -2.47 -1.80 0.76 -2.14 -0.51 -2.64
Eisosomes -0.65 -5.24 -1.81 -2.22 5.62 -7.00 -16.06 -14.34 -6.81 -2.89 -7.60 -17.40 -15.83 -6.56 -0.39
Endoplasmic Reticulum -1.62 -2.60 -1.14 0.92 2.35 -6.32 -6.24 -10.28 -2.92 -6.57 -7.40 -7.25 -11.66 -2.55 -6.45
Endosome -2.02 -1.92 -3.24 -0.74 -2.98 -3.07 1.70 -3.00 -1.63 -3.14 -3.39 1.58 -3.97 -1.66 -4.20
Golgi -1.86 -5.22 -2.04 -0.39 -1.79 -1.92 0.11 -2.29 -1.18 -2.30 -2.29 -0.09 -2.99 -1.28 -3.00
Lipid Particles -0.97 -1.11 -0.05 0.96 1.32 -2.29 1.22 -1.77 -0.13 -2.01 -2.07 0.96 -1.86 1.05 -2.22
Mitochondria -2.44 -4.77 -8.36 -5.58 -7.00 -5.17 -7.49 -9.50 -0.14 -7.42 -5.45 -8.05 -12.30 -2.24 -10.22
Mitotic Spindle -2.07 -1.43 -6.39 -5.39 -3.88 -3.52 -0.46 -6.23 -4.26 -6.18 -3.46 -0.68 -8.23 -5.86 -8.13
None -1.35 -1.17 -2.05 0.65 0.12 0.12 -4.27 -3.92 -3.91 -0.52 -0.47 -4.33 -3.99 -3.39 0.81
Nuclear Periphery -0.22 -0.62 -5.39 -5.56 -5.42 -3.88 0.15 -3.96 -1.76 -4.02 -3.80 0.09 -6.61 -3.91 -6.73
Nuclear Periphery Foci -1.58 -2.21 -3.74 0.67 -3.11 -1.96 1.13 -2.17 -0.87 -2.33 -2.42 1.02 -3.20 -0.55 -3.47
Nucleolus -2.28 -1.06 3.10 7.66 5.28 -2.01 4.16 10.39 11.80 6.04 -2.30 3.94 13.47 15.66 8.97
Nucleus 4.65 3.28 7.60 1.52 3.57 7.36 3.55 9.47 3.88 7.59 8.03 3.93 11.48 4.09 8.93
Peroxisomes -0.95 -2.81 1.52 3.77 5.66 -2.99 -0.66 -3.56 1.91 -3.65 -3.16 -1.69 -3.15 2.44 -2.51
Vacuole -2.58 -4.89 -5.83 -2.84 -3.51 -4.76 -1.40 -5.45 -3.75 -4.80 -5.23 -1.75 -7.63 -4.81 -6.26
Vacuole Periphery -1.97 -5.36 -3.13 -1.61 -2.91 -3.68 1.72 -2.45 -0.96 -2.63 -3.92 1.58 -3.67 -1.47 -3.94
Standard name
Human Ortholog
Description Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Tfb3

Tfb3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tfb3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available