Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07
Bud 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.07 0 0 0 0 – 0 0 0 0 0 0.08 0 0 0.07 0.09 0.08 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.09 0.14 0.12 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.07 0.08 0.05 0 0 0 0 0 0
Golgi 0 0.1 0 0 0 0 – 0 0 0 0 0 0.17 0.11 0.21 0.31 0.28 0.21 0.06 0.08 0.06 0.11 0.14 0.11
Mitochondria 0.92 0.88 0.95 0.97 0.95 0.92 – 0.87 0.95 0.93 0.96 0.96 0.8 0.87 0.82 0.12 0.14 0.08 0.8 0.78 0.77 0.67 0.63 0.64
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.06 0.05 0 0 0.07 0.08 – 0.08 0.13 0.09 0.1 0.09 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.1 – 0.1 0.08 0.08 0.05 0.06 0 0 0 0.05 0 0.11 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0.1 0.07 0.05 0 0.11 0.15 0 0 0.09 0.05 0.08 0.08
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0.11 0 0 0.06 0 – 0 0 0 0 0 0.1 0.07 0.09 0.26 0.09 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 3 2 0 1 0 – 2 0 1 2 0 2 6 1 3 1 4 16 13 6 6 18 31
Bud 14 1 20 18 16 5 – 14 22 11 17 19 1 3 4 2 2 3 3 1 0 1 0 3
Bud Neck 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 2 2
Bud Site 3 1 6 2 3 5 – 5 4 3 7 9 0 0 0 0 0 1 – – – – – –
Cell Periphery 28 25 16 12 8 2 – 3 4 6 9 6 32 16 18 6 8 7 2 1 1 1 0 4
Cytoplasm 1 3 6 7 10 11 – 19 7 7 8 2 5 16 10 7 12 11 0 1 1 1 1 0
Endoplasmic Reticulum 5 5 6 2 6 1 – 2 0 4 2 3 2 2 7 2 2 2 1 0 1 1 1 2
Endosome 10 9 2 1 0 11 – 11 2 5 1 0 17 7 18 6 7 5 26 7 19 10 11 13
Golgi 22 33 2 0 0 0 – 2 0 1 0 2 69 52 106 25 24 19 47 29 31 30 46 54
Mitochondria 670 303 480 418 329 501 – 558 609 669 560 529 324 402 420 10 12 7 630 298 394 184 217 307
Nucleus 8 1 1 1 5 3 – 10 1 5 2 2 1 0 1 2 3 2 2 0 0 1 0 0
Nuclear Periphery 42 18 19 15 24 45 – 50 83 67 58 50 16 16 16 3 5 1 3 3 3 3 2 4
Nucleolus 16 6 15 14 11 57 – 65 49 57 32 32 8 6 5 4 3 10 2 0 1 1 1 3
Peroxisomes 6 6 12 12 2 12 – 11 6 10 5 10 41 33 28 3 9 14 21 15 43 14 26 36
SpindlePole 6 1 2 2 3 4 – 5 7 12 1 6 3 1 5 2 2 6 0 0 1 1 0 2
Vac/Vac Membrane 100 37 18 6 21 14 – 15 20 9 14 11 41 30 45 21 8 13 24 4 2 10 11 12
Unique Cell Count 732 343 503 430 348 545 641 641 723 583 551 404 460 513 81 85 91 790 380 513 274 347 483
Labelled Cell Count 936 452 607 510 439 671 772 815 867 718 681 562 590 684 96 98 105 790 380 513 274 347 483


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 10.4 6.2 7.1 4.6 6.7 – 6.8 6.3 6.4 6.5 6.4 10.0 9.3 9.0 10.6 10.4 9.5 9.5 10.3 10.2
Std Deviation (1e-4) 1.7 1.7 1.2 1.4 1.4 1.5 – 1.8 1.7 1.7 1.8 1.7 8.1 1.6 1.5 2.7 2.5 2.2 2.2 2.1 2.1
Intensity Change (Log2) – – – 0.2 -0.43 0.12 – 0.15 0.02 0.04 0.08 0.05 0.69 0.59 0.54 0.78 0.75 0.62 0.61 0.74 0.73


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0.2 0 -3.2 – -1.8 -0.5 -2.7 -1.0 -0.5 -3.7 -3.4 -3.4 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 -0.4 – 0 0 0 0 0.6 0 0 0 0 0 0
Cell Periphery -0.3 0 -3.5 – -3.6 -3.3 -3.1 -1.8 -2.4 3.2 0.3 0.3 0 0 0
Cytoplasm 0.6 1.8 1.1 – 2.0 -0.2 -0.4 0.3 0 0 2.4 1.0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 0 0 0.2 0 0 0
Endosome 0 0 2.4 – 2.1 0 0 0 0 4.0 0 3.6 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 9.3 7.3 10.5 0 0 0
Mitochondria 1.4 0 -2.3 – -4.8 -0.3 -2.1 0.5 0.5 -7.2 -4.5 -6.8 -18.9 -18.8 -20.0
Nucleus 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.2 0 3.0 – 2.8 5.4 3.7 4.0 3.5 0.1 -0.2 -0.6 0 0 0
Nucleolus 0.2 0 4.8 – 4.7 3.4 3.6 2.0 2.2 -1.0 -1.8 -2.3 0 0 0
Peroxisomes 0.4 0 -0.2 – -0.8 -2.0 -1.3 -2.0 -0.6 5.0 3.5 2.5 0 0 0
SpindlePole 0 0 0 – 0 0 2.0 0 0 0 0 0 0 0 0
Vacuole -2.1 1.7 -0.9 – -1.2 -0.4 -2.7 -1.1 -1.6 4.0 2.1 3.4 7.5 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.0806 8.4138 7.6187 7.4399 7.9118 8.238 6.4149 6.1441 6.1994 5.4878 5.7546 6.1854 8.8442 8.5795 8.1431 7.6075 7.6492 8.1772
Actin 0.01 0.0129 0.0105 0.0025 0.0026 0.0008 0.006 0.0076 0.0023 0.0029 0.0003 0.0029 0.0132 0.0051 0.0081 0.0021 0.0231 0.0004
Bud 0.0006 0.0002 0.0004 0.0002 0.0002 0 0.0003 0.0001 0 0.0001 0 0 0.0008 0.0004 0.0011 0.0001 0.0001 0
Bud Neck 0.0028 0.0002 0.0027 0.0001 0.0003 0 0.0001 0.0001 0.0003 0 0 0 0.0005 0.0001 0.0014 0 0.0001 0
Bud Periphery 0.0012 0.0004 0.0006 0.0006 0.0003 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0.0019 0.0009 0.0019 0.0002 0.0003 0.0001
Bud Site 0.0036 0.0004 0.0045 0.0002 0.0033 0 0.0008 0.0003 0.0001 0.0001 0 0 0.002 0.0007 0.0049 0.0001 0.0004 0
Cell Periphery 0.0004 0.0001 0.0003 0.0002 0.0003 0.0001 0.0001 0.0001 0.0001 0 0 0 0.0002 0.0002 0.0007 0.0001 0.0001 0.0001
Cytoplasm 0.0034 0.0001 0.0003 0 0.0085 0 0 0 0.0001 0 0 0 0.0032 0.0002 0.0005 0 0 0.0001
Cytoplasmic Foci 0.0211 0.004 0.0059 0.0012 0.0128 0.0005 0.0018 0.0019 0.0011 0.0003 0.0012 0.0007 0.0134 0.0036 0.0086 0.0031 0.0037 0.0007
Eisosomes 0.0004 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0.0001 0.0008 0.0001 0.0003 0.0001
Endoplasmic Reticulum 0.0029 0.0001 0.0003 0.0001 0.0048 0.0001 0 0.0001 0.0003 0 0 0.0001 0.0016 0.0001 0.0015 0 0.0001 0
Endosome 0.0321 0.0082 0.0137 0.0114 0.0568 0.0037 0.0057 0.0052 0.0058 0.0003 0.0045 0.0035 0.0412 0.014 0.0076 0.007 0.0087 0.003
Golgi 0.0185 0.0165 0.0104 0.0223 0.0136 0.0182 0.0095 0.0164 0.0166 0.0012 0.0148 0.0165 0.0215 0.0251 0.0158 0.0171 0.0261 0.0085
Lipid Particles 0.0421 0.0148 0.0095 0.0018 0.0269 0.0038 0.0056 0.0097 0.0023 0.0003 0.0024 0.0103 0.0131 0.012 0.0156 0.006 0.0386 0.0023
Mitochondria 0.7824 0.9293 0.9167 0.9402 0.8223 0.9616 0.9593 0.9489 0.9595 0.9913 0.9733 0.957 0.8525 0.9125 0.9032 0.9561 0.886 0.9765
None 0.0025 0 0.0002 0 0.0039 0 0 0 0.0001 0 0 0 0.0015 0.0001 0.0003 0 0.0001 0.0004
Nuclear Periphery 0.0161 0 0.0027 0.0003 0.0029 0.0002 0 0.0001 0.0012 0 0 0.0002 0.0062 0.0002 0.0006 0 0.0002 0.0001
Nucleolus 0.0016 0.0001 0.0003 0.0001 0.0013 0 0.0001 0 0.0001 0 0 0.0001 0.0013 0.0003 0.0022 0 0.0001 0.0001
Nucleus 0.0097 0.0001 0.0004 0.0001 0.0031 0 0 0 0.0007 0 0 0 0.0025 0.0001 0.0005 0 0 0.0002
Peroxisomes 0.0224 0.0065 0.0094 0.0015 0.0075 0.0072 0.0077 0.0079 0.004 0.0032 0.0024 0.0067 0.0089 0.0077 0.0119 0.0065 0.0095 0.0012
Punctate Nuclear 0.0096 0 0.0012 0 0.0017 0 0 0 0.0001 0 0 0 0.0035 0 0.001 0.0001 0.0001 0.0039
Vacuole 0.007 0.0015 0.0038 0.003 0.0083 0.0006 0.0005 0.0003 0.002 0 0.0002 0.0003 0.0043 0.006 0.0061 0.0004 0.0005 0.0005
Vacuole Periphery 0.0097 0.0044 0.0059 0.0141 0.0185 0.003 0.0017 0.001 0.003 0.0001 0.0005 0.0015 0.0062 0.0103 0.0057 0.0009 0.002 0.0016

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.0643 35.5451 24.7275 30.6994 32.221 39.7403 36.5012 35.6272 51.0431 40.2974
Translational Efficiency 2.2537 2.0546 2.8577 2.7284 2.3732 2.098 2.1182 3.5054 2.1143 2.1448

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1749 1791 1680 1496 224 1818 1607 1266 1973 3609 3287 2762

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 855.73 1008.12 1059.71 1272.75 782.67 966.39 1103.98 1205.37 847.44 987.10 1081.35 1241.87
Standard Deviation 134.80 182.56 201.31 218.08 164.00 172.03 173.76 188.79 140.35 178.55 189.64 207.90
Intensity Change Log 2 — 0.236440 0.308442 0.572721 — 0.304201 0.496238 0.623000 — 0.269207 0.401206 0.596958

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.001021 0.004413 0.002148 0.004367 0.002020 0.004096 0.001529 0.002897 0.001134 0.004254 0.001846 0.003693
Bud Neck 0.000541 0.001001 0.004188 0.001087 0.000795 0.000937 0.003180 0.001156 0.000570 0.000969 0.003695 0.001118
Bud Site 0.000780 0.001086 0.008595 0.002626 0.002424 0.001519 0.006296 0.003396 0.000966 0.001304 0.007471 0.002979
Cell Periphery 0.000258 0.000124 0.000361 0.000215 0.000542 0.000233 0.000169 0.000170 0.000290 0.000179 0.000267 0.000194
Cytoplasm 0.000188 0.000306 0.001007 0.000483 0.002811 0.000389 0.001338 0.000502 0.000486 0.000348 0.001169 0.000491
Cytoplasmic Foci 0.012357 0.015104 0.021405 0.009681 0.007826 0.012848 0.014531 0.013182 0.011843 0.013967 0.018044 0.011286
Eisosomes 0.000045 0.000132 0.000126 0.000154 0.000056 0.000111 0.000073 0.000096 0.000046 0.000121 0.000100 0.000128
Endoplasmic Reticulum 0.000213 0.000133 0.000346 0.000489 0.000711 0.000141 0.000150 0.000345 0.000270 0.000137 0.000250 0.000423
Endosome 0.014642 0.009786 0.024067 0.007301 0.012709 0.016429 0.017239 0.013575 0.014423 0.013132 0.020729 0.010177
Golgi 0.018263 0.028498 0.024679 0.042159 0.015924 0.025719 0.015183 0.057777 0.017998 0.027098 0.020037 0.049318
Lipid Particles 0.007845 0.003815 0.011831 0.003061 0.005579 0.004596 0.004986 0.003359 0.007588 0.004209 0.008485 0.003198
Mitochondria 0.919595* 0.907781* 0.843867* 0.886440* 0.911406* 0.911356* 0.890529* 0.866958* 0.918665* 0.909582* 0.866680* 0.877510*
Mitotic Spindle 0.001239 0.001718 0.007271 0.005253 0.000890 0.001562 0.011077 0.005295 0.001199 0.001639 0.009131 0.005272
None 0.000241 0.000294 0.000802 0.001169 0.000351 0.000285 0.000458 0.000809 0.000253 0.000290 0.000634 0.001004
Nuclear Periphery 0.000133 0.000068 0.001132 0.001178 0.000050 0.000104 0.000573 0.000612 0.000123 0.000086 0.000858 0.000919
Nuclear Periphery Foci 0.000247 0.000101 0.001114 0.000484 0.000199 0.000143 0.000594 0.000488 0.000241 0.000122 0.000859 0.000486
Nucleolus 0.000086 0.000052 0.001251 0.000233 0.000075 0.000251 0.000369 0.000100 0.000085 0.000152 0.000820 0.000172
Nucleus 0.000073 0.000036 0.000426 0.000058 0.000577 0.001111 0.000300 0.000048 0.000130 0.000577 0.000364 0.000053
Peroxisomes 0.015747 0.021371 0.023661 0.004811 0.024219 0.012869 0.018191 0.004147 0.016709 0.017088 0.020987 0.004507
Vacuole 0.002289 0.002015 0.012571 0.009273 0.003857 0.002743 0.007635 0.009519 0.002467 0.002382 0.010158 0.009386
Vacuole Periphery 0.004198 0.002166 0.009152 0.019478 0.006981 0.002560 0.005601 0.015570 0.004514 0.002364 0.007416 0.017687

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -5.22 -3.33 -4.84 0.04 -3.02 -2.33 0.95 -1.02 1.13 -1.79 -6.01 -2.96 -4.94 0.79 -3.49
Bud Neck -1.64 -7.17 -2.77 -0.35 6.07 -0.44 -4.22 -1.23 -0.79 3.64 -1.93 -8.25 -3.20 -0.81 6.84
Bud Site -0.86 -5.97 -3.65 -3.23 4.38 0.71 -2.23 -0.64 -2.17 2.04 -0.93 -7.16 -3.90 -3.52 4.60
Cell Periphery 0.94 -0.69 0.30 -3.90 2.69 1.66 2.06 2.08 1.11 0.03 0.85 0.17 0.75 -0.50 2.34
Cytoplasm -3.52 -3.38 -2.05 -1.34 1.64 1.36 1.12 1.33 -0.79 2.10 1.06 -0.61 0.69 -1.49 2.65
Cytoplasmic Foci -1.70 -4.88 2.00 3.61 6.63 -2.07 -2.71 -2.16 -0.22 0.82 -1.69 -4.56 0.48 2.47 5.59
Eisosomes -7.83 -10.72 -8.75 -1.36 -1.97 -3.65 -1.35 -2.94 1.24 -2.68 -9.81 -10.54 -10.34 -0.60 -3.19
Endoplasmic Reticulum 1.60 -2.28 -2.55 -3.60 -1.38 1.62 1.59 1.04 -4.60 -4.41 2.23 0.31 -1.90 -4.99 -2.90
Endosome 3.02 -4.40 4.68 2.19 9.23 -1.12 -1.34 -0.29 1.46 1.78 0.84 -3.69 2.90 2.65 7.76
Golgi -4.03 -2.56 -6.80 -3.64 -4.68 -2.13 0.15 -7.04 -7.02 -9.72 -4.59 -1.09 -10.64 -7.62 -10.30
Lipid Particles 3.21 -2.34 3.94 0.98 6.31 0.23 0.13 0.52 1.01 1.43 2.79 -0.69 3.69 1.43 5.89
Mitochondria 1.67 9.15 4.40 2.79 -4.74 -0.28 0.98 2.29 5.15 2.52 1.46 8.00 6.26 5.61 -1.68
Mitotic Spindle -0.72 -5.19 -3.56 -3.16 1.33 -1.29 -6.50 -5.01 -3.81 3.27 -0.82 -7.74 -5.08 -4.81 3.32
None -2.76 -12.82 -14.28 -13.41 -4.79 0.63 0.06 -1.08 -10.57 -6.01 -0.56 -7.98 -13.59 -16.90 -7.49
Nuclear Periphery 2.68 -4.94 -3.64 -3.88 -0.14 -2.02 -4.55 -5.29 -4.73 -0.26 1.62 -6.20 -4.86 -5.11 -0.31
Nuclear Periphery Foci 2.13 -5.16 -2.27 -4.38 3.56 1.14 -1.36 -0.92 -3.09 0.60 2.16 -5.09 -2.59 -5.21 3.03
Nucleolus 1.21 -3.49 -1.98 -2.57 3.00 -0.84 -2.06 0.18 1.25 3.98 -0.94 -4.16 -1.77 -0.29 3.65
Nucleus 1.29 -3.11 0.49 -1.60 3.32 0.40 1.00 1.18 1.48 2.36 -0.79 -0.58 1.27 1.45 4.04
Peroxisomes -1.92 -2.73 5.10 7.09 8.09 1.41 0.73 2.52 5.50 7.25 -0.15 -1.78 6.02 8.89 10.80
Vacuole 0.51 -9.39 -5.93 -6.05 2.09 0.73 -1.87 -2.62 -5.55 -1.28 0.23 -10.01 -7.82 -8.17 0.71
Vacuole Periphery 3.58 -5.60 -8.70 -10.13 -5.62 1.60 0.48 -2.83 -9.30 -6.42 3.81 -3.93 -10.68 -13.61 -8.39
Standard name
Human Ortholog
Description Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages mitochondrion (99%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mrpl28

Mrpl28


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrpl28-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available