Standard name | |
---|---|
Human Ortholog | |
Description | Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 1 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 8 | 4 | 9 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 1 | 2 | 12 | 13 | 12 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 5 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 160 | 205 | 158 | 18 | 39 | 68 | 93 | 79 | 59 | 90 | 37 | 52 | 261 | 344 | 289 | 196 | 176 | 148 | 143 | 151 | 140 | 159 | 263 | 349 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 2 | 4 | 3 | 1 | 12 | 8 | 17 | 0 | 0 | 2 | 1 | 2 | 5 |
Endosome | 4 | 0 | 2 | 0 | 3 | 2 | 3 | 8 | 4 | 1 | 0 | 0 | 14 | 0 | 29 | 3 | 3 | 5 | 4 | 3 | 5 | 3 | 9 | 21 |
Golgi | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 25 | 15 | 9 | 1 | 0 | 0 | 0 | 0 | 2 |
Mitochondria | 6 | 5 | 1 | 7 | 17 | 96 | 120 | 135 | 107 | 184 | 110 | 131 | 3 | 0 | 0 | 2 | 1 | 1 | 1 | 2 | 1 | 2 | 3 | 3 |
Nucleus | 9 | 16 | 27 | 44 | 58 | 42 | 56 | 64 | 74 | 80 | 50 | 56 | 54 | 128 | 166 | 24 | 12 | 7 | 72 | 60 | 39 | 142 | 148 | 103 |
Nuclear Periphery | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 7 |
Nucleolus | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 3 | 2 | 0 | 0 | 0 | 1 | 2 | 4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 10 | 19 |
Vac/Vac Membrane | 81 | 29 | 59 | 25 | 95 | 83 | 89 | 79 | 79 | 75 | 38 | 49 | 204 | 278 | 344 | 292 | 220 | 184 | 9 | 7 | 19 | 15 | 33 | 33 |
Unique Cell Count | 218 | 229 | 211 | 87 | 185 | 238 | 290 | 298 | 265 | 353 | 182 | 228 | 442 | 571 | 670 | 442 | 343 | 305 | 246 | 235 | 222 | 349 | 499 | 579 |
Labelled Cell Count | 260 | 257 | 250 | 95 | 213 | 297 | 366 | 378 | 330 | 443 | 241 | 295 | 540 | 754 | 833 | 561 | 441 | 375 | 246 | 235 | 222 | 349 | 499 | 579 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.1 | 8.8 | 8.5 | 8.0 | 7.0 | 5.7 | 5.7 | 5.5 | 5.4 | 5.1 | 4.8 | 4.7 | 10.4 | 10.4 | 9.7 | 17.9 | 17.2 | 16.6 | 11.9 | 11.4 | 11.4 |
Std Deviation (1e-4) | 1.3 | 1.6 | 1.3 | 1.7 | 1.5 | 1.2 | 1.2 | 1.1 | 1.4 | 1.4 | 1.0 | 1.0 | 1.8 | 1.5 | 2.0 | 3.8 | 2.9 | 3.8 | 1.7 | 1.9 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.09 | -0.29 | -0.57 | -0.57 | -0.63 | -0.67 | -0.73 | -0.84 | -0.87 | 0.29 | 0.29 | 0.18 | 1.08 | 1.02 | 0.96 | 0.49 | 0.42 | 0.42 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -8.6 | -10.7 | -9.8 | -9.5 | -10.8 | -11.5 | -11.4 | -10.8 | -10.9 | -3.9 | -3.8 | -8.0 | -7.3 | -5.5 | -6.0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.7 | 0 | 2.3 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.5 | 3.1 | 0 |
Mitochondria | 0 | 4.2 | 10.2 | 10.6 | 11.3 | 10.3 | 12.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 7.0 | 4.5 | 1.4 | 1.9 | 2.5 | 4.0 | 2.9 | 3.7 | 3.1 | -0.2 | 3.0 | 3.7 | -3.3 | -4.2 | -4.7 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0.1 | 4.8 | 1.6 | 0.7 | -0.4 | 0.4 | -1.8 | -1.6 | -1.6 | 4.4 | 5.2 | 5.9 | 9.1 | 8.3 | 7.2 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.7702 | 6.2175 | 6.0582 | 5.5958 | 5.6837 | 5.9261 | 5.4877 | 6.9446 | 6.0432 | 5.53 | 5.1611 | 5.9352 | 4.3565 | 5.0414 | 4.7738 | 5.1994 | 5.1465 | 5.1734 |
Actin | 0.0147 | 0.0006 | 0.0059 | 0.0013 | 0.0128 | 0.0042 | 0.0306 | 0.0051 | 0.0008 | 0.0103 | 0.0071 | 0.0026 | 0.0444 | 0.0004 | 0.0009 | 0.0043 | 0.0175 | 0.0013 |
Bud | 0.0004 | 0.0004 | 0.0003 | 0.0004 | 0.0003 | 0.0002 | 0.0016 | 0.0005 | 0.0013 | 0.0002 | 0.0032 | 0.0002 | 0.0007 | 0.0006 | 0.0002 | 0.0002 | 0.0046 | 0.0002 |
Bud Neck | 0.0028 | 0.0006 | 0.0009 | 0.0015 | 0.0015 | 0.0031 | 0.0017 | 0.0007 | 0.004 | 0.0012 | 0.0023 | 0.0026 | 0.0022 | 0.0018 | 0.0006 | 0.0029 | 0.0023 | 0.0017 |
Bud Periphery | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0011 | 0.0002 | 0.0018 | 0.0001 | 0.0034 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0096 | 0.0001 |
Bud Site | 0.0009 | 0.0012 | 0.0008 | 0.0007 | 0.0004 | 0.0002 | 0.002 | 0.0033 | 0.0059 | 0.001 | 0.0273 | 0.0002 | 0.0045 | 0.0177 | 0.0005 | 0.0003 | 0.021 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0.0011 | 0 | 0.0004 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0004 | 0 |
Cytoplasm | 0.3479 | 0.4733 | 0.4539 | 0.4828 | 0.4034 | 0.4357 | 0.4044 | 0.6694 | 0.4787 | 0.5478 | 0.7008 | 0.5738 | 0.354 | 0.6287 | 0.5355 | 0.4949 | 0.3797 | 0.561 |
Cytoplasmic Foci | 0.0119 | 0.0071 | 0.0035 | 0.0063 | 0.0043 | 0.0047 | 0.0284 | 0.0032 | 0.0036 | 0.0116 | 0.0255 | 0.0029 | 0.0129 | 0.0017 | 0.0141 | 0.0146 | 0.0086 | 0.0018 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0004 | 0 | 0 | 0 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0042 | 0.0023 | 0.0046 | 0.0026 | 0.0076 | 0.0023 | 0.0058 | 0.002 | 0.0021 | 0.001 | 0.0029 | 0.0035 | 0.014 | 0.0021 | 0.0017 | 0.009 | 0.0041 | 0.0034 |
Endosome | 0.0183 | 0.0062 | 0.0048 | 0.0033 | 0.0164 | 0.0053 | 0.027 | 0.0015 | 0.0086 | 0.0072 | 0.0425 | 0.0053 | 0.0142 | 0.002 | 0.0149 | 0.0161 | 0.0248 | 0.0028 |
Golgi | 0.0035 | 0.0003 | 0.0003 | 0.0002 | 0.0008 | 0.0007 | 0.0151 | 0.0003 | 0.0018 | 0.0134 | 0.0269 | 0.0031 | 0.0025 | 0.0001 | 0.0016 | 0.0012 | 0.0032 | 0.0001 |
Lipid Particles | 0.0042 | 0.0001 | 0.0004 | 0.0001 | 0.0005 | 0.0009 | 0.0171 | 0.0001 | 0.004 | 0.0103 | 0.011 | 0.0076 | 0.0096 | 0 | 0.0029 | 0.0014 | 0.0056 | 0.0001 |
Mitochondria | 0.0063 | 0.0008 | 0.0056 | 0.0006 | 0.0012 | 0.0039 | 0.0041 | 0.0007 | 0.001 | 0.0021 | 0.022 | 0.0023 | 0.0035 | 0.0003 | 0.0122 | 0.0006 | 0.037 | 0.0005 |
None | 0.0172 | 0.0187 | 0.0185 | 0.0256 | 0.0226 | 0.016 | 0.0092 | 0.003 | 0.0013 | 0.0013 | 0.0014 | 0.0023 | 0.0271 | 0.0039 | 0.0071 | 0.0129 | 0.0033 | 0.0022 |
Nuclear Periphery | 0.0161 | 0.0144 | 0.0152 | 0.0093 | 0.0375 | 0.0113 | 0.0199 | 0.0076 | 0.0093 | 0.0069 | 0.0062 | 0.0133 | 0.0392 | 0.0091 | 0.0082 | 0.0165 | 0.0291 | 0.0169 |
Nucleolus | 0.0011 | 0.0008 | 0.0007 | 0.0006 | 0.0012 | 0.001 | 0.0015 | 0.0003 | 0.001 | 0.0002 | 0.0014 | 0.0006 | 0.002 | 0.0003 | 0.0004 | 0.0003 | 0.002 | 0.0003 |
Nucleus | 0.5221 | 0.456 | 0.4691 | 0.4471 | 0.4691 | 0.491 | 0.3802 | 0.2968 | 0.4606 | 0.37 | 0.0972 | 0.3604 | 0.4305 | 0.3273 | 0.3876 | 0.4073 | 0.4181 | 0.4016 |
Peroxisomes | 0.0055 | 0.0011 | 0.0003 | 0.0004 | 0.0004 | 0.0031 | 0.0124 | 0.0005 | 0.001 | 0.0114 | 0.0112 | 0.0007 | 0.0049 | 0.0001 | 0.0021 | 0.0009 | 0.0028 | 0.0001 |
Punctate Nuclear | 0.0159 | 0.0127 | 0.0123 | 0.0164 | 0.016 | 0.0144 | 0.0329 | 0.0038 | 0.0022 | 0.0032 | 0.0016 | 0.0166 | 0.0288 | 0.0024 | 0.0079 | 0.0145 | 0.0184 | 0.0041 |
Vacuole | 0.0045 | 0.0029 | 0.0019 | 0.0006 | 0.003 | 0.0015 | 0.0037 | 0.001 | 0.0092 | 0.0006 | 0.0048 | 0.0013 | 0.0024 | 0.0013 | 0.0013 | 0.0015 | 0.003 | 0.0011 |
Vacuole Periphery | 0.0014 | 0.0005 | 0.0004 | 0.0002 | 0.001 | 0.0003 | 0.001 | 0.0002 | 0.0006 | 0.0002 | 0.0011 | 0.0005 | 0.0015 | 0.0002 | 0.0002 | 0.0004 | 0.0047 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 30.7243 | 28.6598 | 32.491 | 34.0838 | 33.4849 | 21.3616 | 27.7705 | 27.4357 | 27.6499 | 31.4855 |
Translational Efficiency | 2.111 | 2.1329 | 1.6329 | 1.2582 | 1.1904 | 2.0358 | 1.5532 | 1.1741 | 1.082 | 1.1758 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
338 | 123 | 2565 | 1438 | 1052 | 1898 | 285 | 351 | 1390 | 2021 | 2850 | 1789 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1067.37 | 1140.09 | 1203.85 | 1316.52 | 876.99 | 1016.57 | 1298.64 | 1364.89 | 923.28 | 1024.09 | 1213.33 | 1326.01 |
Standard Deviation | 217.98 | 177.08 | 172.00 | 180.18 | 141.57 | 148.48 | 138.28 | 191.61 | 182.73 | 153.25 | 171.31 | 183.49 |
Intensity Change Log 2 | — | 0.095087 | 0.173595 | 0.302669 | — | 0.213077 | 0.566369 | 0.638152 | — | 0.149503 | 0.364069 | 0.463696 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000482 | 0.000587 | 0.000902 | 0.003298 | 0.000471 | 0.000302 | 0.001464 | 0.001526 | 0.000473 | 0.000319 | 0.000958 | 0.002950 |
Bud Neck | 0.004260 | 0.001667 | 0.009007 | 0.014445 | 0.001498 | 0.004174 | 0.011379 | 0.008493 | 0.002170 | 0.004021 | 0.009245 | 0.013277 |
Bud Site | 0.003049 | 0.002847 | 0.007960 | 0.013019 | 0.002467 | 0.005628 | 0.003924 | 0.026386 | 0.002608 | 0.005458 | 0.007556 | 0.015642 |
Cell Periphery | 0.000200 | 0.000148 | 0.000085 | 0.000245 | 0.000176 | 0.000079 | 0.000222 | 0.000246 | 0.000182 | 0.000084 | 0.000099 | 0.000245 |
Cytoplasm | 0.293822* | 0.426296* | 0.433096* | 0.210909* | 0.409473* | 0.481316* | 0.068539 | 0.336287* | 0.381351* | 0.477968* | 0.396640* | 0.235508* |
Cytoplasmic Foci | 0.013435 | 0.022596 | 0.003679 | 0.018661 | 0.011148 | 0.012887 | 0.001509 | 0.026817 | 0.011704 | 0.013478 | 0.003462 | 0.020261 |
Eisosomes | 0.000035 | 0.000032 | 0.000013 | 0.000030 | 0.000016 | 0.000013 | 0.000066 | 0.000028 | 0.000020 | 0.000014 | 0.000019 | 0.000029 |
Endoplasmic Reticulum | 0.007333 | 0.004837 | 0.001352 | 0.004385 | 0.003605 | 0.002113 | 0.015964 | 0.002690 | 0.004511 | 0.002279 | 0.002813 | 0.004053 |
Endosome | 0.002309 | 0.002338 | 0.002033 | 0.014699 | 0.001136 | 0.002571 | 0.002098 | 0.010384 | 0.001421 | 0.002557 | 0.002039 | 0.013852 |
Golgi | 0.000454 | 0.001559 | 0.000411 | 0.018033 | 0.000149 | 0.001550 | 0.000132 | 0.002941 | 0.000223 | 0.001550 | 0.000383 | 0.015072 |
Lipid Particles | 0.001408 | 0.001304 | 0.000454 | 0.004023 | 0.000512 | 0.000850 | 0.001468 | 0.001177 | 0.000730 | 0.000877 | 0.000555 | 0.003465 |
Mitochondria | 0.003050 | 0.000401 | 0.001590 | 0.018860 | 0.000433 | 0.002189 | 0.001652 | 0.001104 | 0.001069 | 0.002080 | 0.001597 | 0.015377 |
Mitotic Spindle | 0.004726 | 0.010367 | 0.026621 | 0.099106 | 0.000224 | 0.013779 | 0.020299 | 0.069567 | 0.001319 | 0.013571 | 0.025989 | 0.093310 |
None | 0.003175 | 0.005597 | 0.009124 | 0.009879 | 0.010472 | 0.006184 | 0.002015 | 0.021554 | 0.008697 | 0.006148 | 0.008413 | 0.012170 |
Nuclear Periphery | 0.001030 | 0.000647 | 0.001346 | 0.003481 | 0.000471 | 0.001034 | 0.002694 | 0.001350 | 0.000607 | 0.001011 | 0.001481 | 0.003063 |
Nuclear Periphery Foci | 0.002500 | 0.003544 | 0.000555 | 0.002853 | 0.001339 | 0.001104 | 0.002494 | 0.001101 | 0.001621 | 0.001253 | 0.000749 | 0.002510 |
Nucleolus | 0.000522 | 0.000684 | 0.000647 | 0.001376 | 0.000483 | 0.001314 | 0.000671 | 0.000692 | 0.000493 | 0.001276 | 0.000650 | 0.001242 |
Nucleus | 0.615455* | 0.470570* | 0.464283* | 0.486201* | 0.544468* | 0.435006* | 0.781487* | 0.384376* | 0.561729* | 0.437171* | 0.496003* | 0.466223* |
Peroxisomes | 0.000238 | 0.000540 | 0.000564 | 0.001236 | 0.000229 | 0.000794 | 0.000189 | 0.001185 | 0.000231 | 0.000779 | 0.000527 | 0.001226 |
Vacuole | 0.042140 | 0.043226 | 0.034970 | 0.066403 | 0.010936 | 0.026557 | 0.081538 | 0.098564 | 0.018524 | 0.027572 | 0.039626 | 0.072713 |
Vacuole Periphery | 0.000377 | 0.000213 | 0.001307 | 0.008858 | 0.000295 | 0.000555 | 0.000195 | 0.003530 | 0.000315 | 0.000534 | 0.001195 | 0.007812 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -1.36 | 0.07 | -3.00 | -1.42 | -2.12 | 1.75 | -9.73 | -4.47 | -5.80 | 3.42 | 1.97 | -0.90 | -4.17 | -5.28 | -1.95 |
Bud Neck | 1.86 | -3.21 | -5.60 | -9.17 | -2.71 | -5.83 | -2.87 | -12.34 | -6.76 | -0.50 | -4.17 | -7.80 | -12.09 | -7.97 | -2.80 |
Bud Site | -0.91 | -2.82 | -5.31 | -3.32 | -1.68 | -2.89 | -3.62 | -5.31 | -3.47 | -3.96 | -2.93 | -3.76 | -7.51 | -2.88 | -2.95 |
Cell Periphery | 0.96 | 5.82 | 1.10 | -0.25 | -10.05 | 6.75 | -3.81 | -2.50 | -7.74 | 0.80 | 7.90 | 6.90 | -0.59 | -10.89 | -10.09 |
Cytoplasm | -3.65 | -3.83 | 6.71 | 8.16 | 24.50 | -3.98 | 29.58 | 18.01 | 24.27 | -8.31 | -6.93 | 6.46 | 22.36 | 34.47 | 22.34 |
Cytoplasmic Foci | -1.28 | 6.82 | 5.97 | 3.62 | -1.72 | -1.01 | 7.99 | 4.17 | 5.78 | -2.57 | -1.01 | 9.67 | 7.62 | 9.53 | -2.50 |
Eisosomes | 0.13 | 6.33 | 2.97 | 1.60 | -9.88 | 1.37 | -12.69 | -10.45 | -11.61 | 5.77 | 4.19 | -0.41 | -6.42 | -13.04 | -7.41 |
Endoplasmic Reticulum | 1.90 | 7.74 | 5.82 | 2.69 | -7.25 | 4.20 | -11.90 | 0.49 | -3.09 | 11.94 | 6.66 | 4.56 | 4.46 | -3.13 | -0.26 |
Endosome | -0.27 | 3.35 | 0.59 | 0.77 | -2.52 | -2.28 | -3.62 | -1.18 | 0.96 | 1.71 | -1.89 | 0.95 | -1.44 | 0.51 | -2.37 |
Golgi | -0.86 | 1.22 | -2.05 | -0.14 | -2.38 | -2.78 | -0.86 | -1.78 | 2.15 | -1.57 | -2.78 | 0.26 | -2.41 | -0.42 | -2.43 |
Lipid Particles | -0.10 | 2.83 | -0.59 | -0.43 | -4.66 | -1.47 | -8.85 | -10.28 | -4.51 | -1.57 | -0.66 | 0.53 | -4.86 | -3.01 | -5.48 |
Mitochondria | 1.06 | 0.50 | -0.67 | -4.09 | -2.80 | -2.95 | -6.52 | -9.14 | 1.10 | 0.27 | -1.38 | -1.27 | -3.03 | -1.89 | -2.76 |
Mitotic Spindle | -1.07 | -6.09 | -9.42 | -4.79 | -5.57 | -6.67 | -2.09 | -6.31 | -4.67 | -5.01 | -6.11 | -10.54 | -13.06 | -8.77 | -6.83 |
None | -2.69 | -15.51 | -9.32 | -4.52 | -1.04 | 3.71 | 6.88 | -2.57 | -5.25 | -7.26 | 2.74 | -0.84 | -3.03 | -6.99 | -3.32 |
Nuclear Periphery | 1.79 | -9.85 | -19.41 | -19.63 | -14.70 | -4.39 | -13.90 | -15.18 | -10.01 | -2.65 | -3.53 | -22.52 | -27.13 | -16.60 | -15.36 |
Nuclear Periphery Foci | -1.46 | 4.51 | 2.57 | 2.91 | -8.04 | -0.04 | -4.26 | -3.04 | -2.87 | 1.37 | 0.67 | 5.23 | 0.01 | -0.74 | -6.76 |
Nucleolus | -1.03 | -0.65 | -2.97 | -0.88 | -2.22 | -2.32 | -1.40 | -3.60 | 1.37 | -1.16 | -2.33 | -1.24 | -4.06 | 1.22 | -2.44 |
Nucleus | 3.96 | 3.94 | 1.49 | -3.53 | -4.66 | 8.04 | -10.64 | 3.50 | -1.86 | 11.65 | 10.08 | 0.31 | -0.10 | -11.42 | -0.48 |
Peroxisomes | -1.11 | 0.09 | 0.36 | 1.18 | 0.12 | -2.23 | 0.56 | -0.73 | 1.96 | -1.38 | -2.32 | 0.01 | -0.06 | 2.31 | -0.05 |
Vacuole | -0.41 | -0.15 | -9.54 | -6.43 | -13.92 | -11.42 | -10.95 | -16.26 | -14.02 | -5.90 | -6.37 | -13.78 | -25.34 | -21.48 | -16.22 |
Vacuole Periphery | 1.11 | -0.43 | -1.45 | -1.71 | -1.03 | -1.49 | 1.30 | 0.01 | 1.03 | -0.57 | -1.39 | -0.56 | -1.55 | -1.08 | -0.97 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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