Standard name
Human Ortholog
Description Subunit of the COMPASS (Set1C) complex; COMPASS methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0.08 0.06 – 0.05 0 0 0.05 0.05 0.11 0.14 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0.09 0 0 0 0.28 0 – 0.18 0.22 0.28 0.23 0 0 0 0 0.06 0 0 0 0 0 0 0
Nucleus 0.84 0.91 0.91 0.93 0.89 0.75 0.85 – 0.75 0.8 0.8 0.72 0.94 0.86 0.8 0.87 0.87 0.88 0.65 0.83 0.78 0.74 0.74 0.61
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.15 0.1 0.06 0.06 0 0.07 0.07 – 0.15 0.15 0.19 0.15 0.08 0.06 0 0.08 0.06 0.08 0.16 0.09 0.11 0.15 0.15 0.19
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 3 0 0 0 1 1
Bud 0 0 0 0 0 2 2 – 2 3 1 2 0 0 0 0 0 1 6 1 1 0 3 4
Bud Neck 0 0 1 0 0 0 0 – 0 0 1 2 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 1 0 0 0 4 2 – 7 9 10 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 12 6 6 6 19 29 27 – 15 13 8 17 11 39 48 10 6 5 1 0 1 0 0 1
Endoplasmic Reticulum 0 0 0 1 0 0 0 – 1 0 0 0 0 0 2 5 2 1 0 0 0 0 0 0
Endosome 7 1 0 2 14 2 11 – 2 2 0 2 0 1 7 2 2 3 9 1 0 1 1 5
Golgi 2 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 0 4 1 0 0 0 0
Mitochondria 13 22 1 5 4 97 11 – 53 86 62 73 2 0 0 11 10 6 4 1 0 0 0 3
Nucleus 241 212 133 199 335 263 389 – 217 314 180 227 211 297 267 219 153 150 211 209 126 61 156 139
Nuclear Periphery 0 0 2 0 6 3 0 – 4 4 0 3 0 3 1 1 2 2 0 0 0 0 0 0
Nucleolus 44 23 9 12 9 25 34 – 44 59 43 49 19 19 13 21 11 14 52 22 18 12 31 43
Peroxisomes 1 0 0 0 1 0 0 – 1 0 0 0 0 1 4 1 0 0 0 0 0 0 0 1
SpindlePole 10 0 3 9 4 5 14 – 12 8 2 7 2 5 3 0 6 1 14 2 5 0 5 5
Vac/Vac Membrane 7 1 2 2 10 8 7 – 8 2 2 7 0 2 4 5 6 7 12 8 5 4 9 15
Unique Cell Count 288 233 146 215 378 351 460 291 392 224 317 224 344 334 253 175 170 325 252 162 83 211 227
Labelled Cell Count 337 266 157 236 402 438 497 366 500 309 398 245 367 349 276 199 190 325 252 162 83 211 227


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.1 5.9 6.5 6.0 7.3 5.0 6.1 – 5.3 5.4 5.1 5.5 6.9 6.9 6.8 8.7 8.8 8.7 6.0 6.3 6.4
Std Deviation (1e-4) 1.4 1.2 1.0 1.4 2.3 1.5 1.7 – 1.9 1.3 1.6 1.4 1.7 1.8 1.9 1.9 2.2 2.1 1.5 1.6 1.5
Intensity Change (Log2) – – – -0.1 0.17 -0.38 -0.1 – -0.28 -0.25 -0.33 -0.24 0.1 0.09 0.08 0.43 0.45 0.43 -0.12 -0.03 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.4 1.6 0.8 – 0.5 -0.4 -0.3 0.6 0.4 2.5 3.3 -0.1 -0.3 -0.6
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 6.9 0 – 5.3 6.0 6.8 6.1 0 0 0 0 2.5 0
Nucleus 0.5 -0.8 -4.1 -2.0 – -4.1 -3.0 -2.8 -4.7 1.1 -1.5 -3.0 -1.4 -1.0 -0.8
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.2 -2.1 0.4 0.5 – 2.7 2.8 3.5 2.8 0.8 -0.3 -1.1 0.8 0 0.7
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 1.1 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8909 1.2511 1.1887 0.9745 0.4957 1.2922 1.9383 2.6627 2.3498 1.99 2.3028 2.431 3.6623 4.4249 3.6726 3.8307 3.7912 3.6299
Actin 0.0043 0 0.0002 0.0001 0.0001 0.0001 0.0006 0 0 0 0 0.0007 0.002 0 0.014 0.001 0.0158 0.0004
Bud 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0003 0.0001
Bud Neck 0.0006 0.0001 0.0001 0.0002 0.0009 0.0006 0.0008 0 0.0001 0.0001 0.0008 0.0009 0.0003 0.0001 0.0003 0.0002 0.0016 0.0018
Bud Periphery 0.0002 0 0 0 0.0001 0 0.0003 0 0 0 0.0001 0.0001 0.0001 0 0.0001 0 0.0008 0.0003
Bud Site 0.0004 0 0 0 0.0002 0 0.0013 0 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0005 0.0001 0.0008 0.0004
Cell Periphery 0.0001 0 0 0 0 0 0.0002 0 0 0 0 0 0.0001 0 0 0 0.0001 0.0001
Cytoplasm 0.0034 0.0002 0.0001 0.0025 0.0001 0 0.0008 0.0001 0.0001 0.0001 0.0002 0 0.0079 0.0003 0.0025 0.0003 0.002 0.0003
Cytoplasmic Foci 0.0037 0 0.0007 0.0011 0 0 0.0013 0 0 0 0 0.0001 0.0169 0 0.0022 0.0264 0.0022 0.0002
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0028 0 0 0.0003 0 0.0001 0.0003 0 0 0 0 0 0.0027 0 0.0001 0 0.002 0.0001
Endosome 0.0094 0 0.0001 0.0011 0.0001 0.0004 0.0009 0 0 0 0 0.0002 0.0171 0.0001 0.0006 0.0033 0.0149 0.0006
Golgi 0.0014 0 0.0001 0.0001 0 0.0001 0.0002 0 0 0 0 0.0002 0.0012 0 0.0016 0.0036 0.0022 0.0001
Lipid Particles 0.0039 0 0.0015 0.0005 0 0.0002 0.0014 0 0 0 0 0.0011 0.0043 0 0.0021 0.0298 0.0043 0.0002
Mitochondria 0.0009 0.0001 0.0001 0.0001 0.0003 0.0002 0.0051 0.0002 0.0001 0.0001 0.0002 0.0011 0.0012 0.0002 0.0007 0.0005 0.0017 0.0107
None 0.0031 0 0.0001 0.0051 0.0001 0 0.0019 0 0 0.0001 0 0 0.0253 0.0001 0.0022 0.0004 0.0023 0.0002
Nuclear Periphery 0.0138 0.002 0.0013 0.0037 0.0026 0.0049 0.001 0.0034 0.0012 0.0006 0.002 0.0006 0.0213 0.0019 0.0012 0.0006 0.0364 0.0014
Nucleolus 0.0339 0.0212 0.0198 0.0181 0.1202 0.0733 0.0351 0.0234 0.0254 0.0181 0.0978 0.0712 0.0259 0.026 0.0327 0.0098 0.1224 0.0782
Nucleus 0.8943 0.9746 0.9661 0.9443 0.8618 0.917 0.9381 0.9705 0.9675 0.9789 0.8399 0.9209 0.8546 0.9646 0.9286 0.905 0.729 0.8914
Peroxisomes 0.0057 0 0.0069 0 0 0 0.001 0 0 0 0 0.0005 0.0011 0 0.0051 0.0175 0.0017 0.0002
Punctate Nuclear 0.0162 0.0015 0.0028 0.0225 0.0133 0.0025 0.0084 0.0022 0.0053 0.0019 0.0586 0.002 0.0155 0.0063 0.0054 0.0013 0.057 0.012
Vacuole 0.001 0.0001 0 0.0002 0.0001 0.0001 0.0005 0 0 0 0.0001 0.0001 0.0012 0.0001 0.0001 0.0001 0.0014 0.0005
Vacuole Periphery 0.0007 0 0 0.0001 0.0001 0.0003 0.0004 0.0001 0 0 0 0.0001 0.0008 0 0 0 0.0011 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 24.0325 22.4176 16.6349 23.7502 12.1927 10.4431 22.6665 23.5849 18.3003 28.5535
Translational Efficiency 1.6896 1.6743 1.6044 1.1677 3.3178 3.6532 1.6109 1.3693 1.5647 1.238

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1910 1171 2686 72 1554 2106 607 779 3464 3277 3293 851

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 753.57 814.27 956.34 1039.49 762.22 891.24 996.89 949.51 757.45 863.74 963.81 957.12
Standard Deviation 102.93 163.39 124.89 177.51 105.95 113.56 130.24 147.16 104.38 138.52 126.87 152.04
Intensity Change Log 2 — 0.111766 0.343782 0.464062 — 0.225607 0.387227 0.316976 — 0.170133 0.365792 0.391973

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000351 0.001333 0.000627 0.002802 0.000446 0.000476 0.001270 0.001546 0.000393 0.000782 0.000746 0.001652
Bud Neck 0.005121 0.013778 0.032934 0.017710 0.005809 0.012889 0.010966 0.033364 0.005430 0.013207 0.028885 0.032040
Bud Site 0.000760 0.002774 0.002492 0.012842 0.000844 0.001912 0.002309 0.024178 0.000797 0.002220 0.002458 0.023219
Cell Periphery 0.000142 0.000225 0.000065 0.000118 0.000101 0.000091 0.000084 0.000279 0.000123 0.000139 0.000069 0.000265
Cytoplasm 0.007934 0.017445 0.002421 0.006457 0.006638 0.005340 0.003743 0.043312 0.007353 0.009665 0.002665 0.040194
Cytoplasmic Foci 0.002031 0.004132 0.000199 0.004507 0.001112 0.001623 0.000199 0.006632 0.001619 0.002519 0.000199 0.006452
Eisosomes 0.000022 0.000044 0.000027 0.000040 0.000027 0.000030 0.000047 0.000046 0.000025 0.000035 0.000031 0.000046
Endoplasmic Reticulum 0.002433 0.004975 0.002436 0.004927 0.002330 0.003055 0.005355 0.006405 0.002387 0.003741 0.002974 0.006280
Endosome 0.000373 0.001657 0.000253 0.004106 0.000384 0.000970 0.000519 0.014166 0.000378 0.001215 0.000302 0.013315
Golgi 0.000243 0.000940 0.000157 0.000841 0.000182 0.000565 0.000351 0.007919 0.000216 0.000699 0.000193 0.007320
Lipid Particles 0.000426 0.001644 0.000066 0.000559 0.000703 0.000883 0.000304 0.000586 0.000550 0.001155 0.000110 0.000584
Mitochondria 0.001181 0.003833 0.001784 0.002041 0.001721 0.004429 0.001289 0.011049 0.001423 0.004216 0.001693 0.010287
Mitotic Spindle 0.000860 0.005918 0.004017 0.139870* 0.002377 0.009730 0.007374 0.053775 0.001541 0.008368 0.004636 0.061059
None 0.015226 0.023877 0.002571 0.005339 0.007822 0.004735 0.006826 0.011755 0.011904 0.011576 0.003355 0.011212
Nuclear Periphery 0.000482 0.000756 0.000578 0.000967 0.000586 0.001001 0.000674 0.002329 0.000529 0.000914 0.000595 0.002214
Nuclear Periphery Foci 0.001362 0.001790 0.000159 0.000287 0.000825 0.000695 0.000522 0.001319 0.001121 0.001086 0.000226 0.001232
Nucleolus 0.072525 0.078525 0.043987 0.015202 0.056376 0.062663 0.047222 0.014744 0.065280 0.068331 0.044583 0.014783
Nucleus 0.887421* 0.832531* 0.903850* 0.772971* 0.909986* 0.885208* 0.906602* 0.729675* 0.897544* 0.866384* 0.904358* 0.733338*
Peroxisomes 0.000428 0.001999 0.000270 0.002676 0.001026 0.000951 0.000781 0.001131 0.000696 0.001326 0.000365 0.001261
Vacuole 0.000414 0.001291 0.000861 0.005431 0.000432 0.001759 0.002113 0.027868 0.000422 0.001592 0.001092 0.025970
Vacuole Periphery 0.000265 0.000532 0.000245 0.000309 0.000271 0.000996 0.001450 0.007922 0.000268 0.000830 0.000467 0.007278

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.26 -14.48 -4.22 -2.30 -3.53 -0.44 -9.85 -6.93 -7.17 -2.22 -4.19 -10.83 -8.38 -5.92 -6.01
Bud Neck -8.82 -28.47 -4.10 -1.92 3.34 -6.64 -6.22 -13.18 -9.84 -8.41 -10.77 -28.28 -14.18 -10.06 -0.98
Bud Site -5.69 -5.95 -2.53 -2.05 -2.08 -3.44 -3.10 -6.33 -5.89 -5.47 -6.41 -6.59 -6.68 -6.03 -5.79
Cell Periphery -2.56 4.36 -0.60 1.78 -3.98 0.50 1.26 -5.31 -5.53 -6.69 -0.96 4.95 -4.36 -3.44 -8.32
Cytoplasm -4.22 6.43 -0.45 2.59 -2.77 0.66 2.61 -8.02 -8.35 -9.07 -2.38 7.11 -7.94 -6.94 -9.65
Cytoplasmic Foci -1.81 3.37 0.48 1.88 -1.56 -1.56 4.19 -2.74 -1.27 -5.14 -1.77 4.48 -1.52 0.06 -5.29
Eisosomes -8.46 -5.62 -5.06 -2.59 -4.40 -1.13 -8.65 -9.95 -9.33 -3.56 -6.68 -7.02 -12.28 -8.66 -9.99
Endoplasmic Reticulum -9.62 -2.05 -5.14 -3.36 -4.96 -3.41 -10.97 -11.71 -10.29 -5.68 -8.36 -7.35 -12.59 -9.97 -11.04
Endosome -3.24 2.18 -2.07 -0.70 -2.18 -3.22 -0.69 -4.59 -4.06 -4.50 -4.58 1.89 -4.76 -3.90 -4.84
Golgi -3.63 0.81 -1.26 1.87 -1.65 -1.98 -1.38 -3.03 -2.55 -2.82 -3.39 -0.08 -3.01 -2.39 -3.01
Lipid Particles -2.47 5.21 -2.13 1.21 -3.81 -1.21 2.96 -0.04 1.36 -4.53 -3.00 6.48 -1.93 2.16 -9.62
Mitochondria -3.46 -3.81 -2.59 0.67 -1.64 -3.27 0.39 -3.73 -1.47 -4.44 -5.15 -1.39 -4.48 -1.62 -4.21
Mitotic Spindle -3.57 -4.75 -2.89 -2.68 -2.72 -3.71 -2.65 -7.75 -6.16 -5.24 -5.31 -4.70 -8.46 -6.99 -7.43
None -2.86 10.39 7.67 7.19 -1.72 3.42 1.62 -0.21 -2.77 -1.52 0.43 11.30 3.46 2.66 -4.44
Nuclear Periphery -2.83 -3.13 -3.97 -3.48 -3.65 -2.45 -0.88 -11.15 -8.76 -9.58 -3.61 -2.48 -12.46 -10.12 -11.81
Nuclear Periphery Foci -0.77 2.89 0.22 0.97 -2.31 1.22 1.36 -0.73 -2.90 -3.07 0.73 3.54 -0.60 -1.64 -5.02
Nucleolus -1.23 6.45 13.01 13.14 9.17 -1.74 0.34 14.47 17.04 9.10 -0.86 5.17 21.72 22.27 16.07
Nucleus 7.89 -0.94 2.62 1.03 2.75 4.57 2.58 13.74 11.28 10.90 7.35 1.69 13.12 9.72 12.52
Peroxisomes -4.89 1.89 -1.52 0.79 -1.71 -0.17 0.46 0.89 1.84 1.29 -2.88 1.58 -0.09 3.23 -2.31
Vacuole -4.54 -2.69 -2.89 -2.80 -2.85 -3.96 -4.15 -9.77 -9.41 -9.18 -5.40 -4.59 -10.19 -9.85 -9.98
Vacuole Periphery -3.11 0.36 -0.42 3.48 -1.08 -2.96 -0.90 -3.89 -2.72 -1.89 -3.49 -0.68 -3.90 -2.95 -3.64
Standard name
Human Ortholog
Description Subunit of the COMPASS (Set1C) complex; COMPASS methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30
Localization
Cell Percentages nucleus (97%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Sdc1

Sdc1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sdc1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available